Skin invasion is the initial step in infection of the human host by schistosome blood flukes. Schistosome larvae have the remarkable ability to overcome the physical and biochemical barriers present in skin in the absence of any mechanical trauma. While a serine peptidase with activity against insoluble elastin appears to be essential for this process in one species of schistosomes, Schistosoma mansoni, it is unknown whether other schistosome species use the same peptidase to facilitate entry into their hosts.
Recent genome sequencing projects, together with a number of biochemical studies, identified alternative peptidases that Schistosoma japonicum or Trichobilharzia regenti could use to facilitate migration through skin. In this study, we used comparative proteomic analysis of human skin treated with purified cercarial elastase, the known invasive peptidase of S. mansoni, or S. mansoni cathespin B2, a close homolog of the putative invasive peptidase of S. japonicum, to identify substrates of either peptidase. Select skin proteins were then confirmed as substrates by in vitro digestion assays.
This study demonstrates that an S. mansoni ortholog of the candidate invasive peptidase of S. japonicum and T. regenti, cathepsin B2, is capable of efficiently cleaving many of the same host skin substrates as the invasive serine peptidase of S. mansoni, cercarial elastase. At the same time, identification of unique substrates and the broader species specificity of cathepsin B2 suggest that the cercarial elastase gene family amplified as an adaptation of schistosomes to human hosts.
Schistosome parasites are a major cause of disease in the developing world, but the mechanism by which these parasites first infect their host has been studied at the molecular level only for S. mansoni. In this paper, we have mined recent genome annotations of S. mansoni and S. japonicum, a zoonotic schistosome species, to identify differential expansion of peptidase gene families that may be involved in parasite invasion and subsequent migration through skin. Having identified a serine peptidase gene family in S. mansoni and a cysteine peptidase gene family in S. japonicum, we then used a comparative proteomic approach to identify potential substrates of representative members of both classes of enzymes from S. mansoni in human skin. The results of this study suggest that while these species evolved to use different classes of peptidases in host invasion, both are capable of cleaving components of the epidermis and dermal extracellular matrix, as well as proteins involved in the host immune response against the migrating parasite.
Citation: Ingram J, Knudsen G, Lim KC, Hansell E, Sakanari J, McKerrow J (2011) Proteomic Analysis of Human Skin Treated with Larval Schistosome Peptidases Reveals Distinct Invasion Strategies among Species of Blood Flukes. PLoS Negl Trop Dis 5(9): e1337. https://doi.org/10.1371/journal.pntd.0001337
Editor: Malcolm K. Jones, University of Queensland, Australia
Received: May 16, 2011; Accepted: August 11, 2011; Published: September 27, 2011
Copyright: © 2011 Ingram et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by a National Science Foundation Predoctoral Fellowship (http://www.nsfgrfp.org/) (JI) and the Sandler Foundation (http://sandler.cgl.ucsf.edu/) (JM). Mass spectrometry was provided by the Bio-Organic Biomedical Mass Spectrometry Resource at the University of California San Francisco (UCSF) (A.L. Burlingame, Director), supported by the Biomedical Research Technology Program of the National Institutes of Health National Center for Research Resources, NIH NCRR P41RR001614 (http://www.ncrr.nih.gov/biomedical_technology/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Human skin is a formidable barrier for much of the microbial world. In addition to the mechanical barrier of structural proteins in the epidermis, basement membrane and dermal extracellular matrix, both the epidermis and dermis are bathed in plasma proteins, including early sentinels of the immune system . In order to successfully breach this barrier, an invading pathogen must degrade protein matrices while minimizing the immune response that it elicits. To this end, many invading organisms utilize insect bites or other mechanical trauma to facilitate their entry into skin, but the multi-cellular larvae of the schistosome blood fluke—the causative agent of the disease schistosomiasis—have the remarkable ability to directly penetrate host skin and gain access to dermal blood vessels , .
The invasive larva(e)—termed cercaria(e)—is 300 µm long, 70 µm wide and comprised of roughly 1000 cells . Upon direct contact with the surface of human skin, cercariae begin to secrete vesicles containing a variety of proteins and an adhesive, mucin-like substance . Proteomic studies identified the majority of proteins secreted by S. mansoni cercariae. These include histolytic peptidases , . The most abundant peptidase in S. mansoni secretions is an S1A serine peptidase, termed cercarial elastase (SmCE) (GenBank: AAC46967.1) that has activity against insoluble elastin and other fibrillar macromolecules of skin . Biochemical and immunolocalization studies have confirmed SmCE activity in cercarial secretions , . Moreover, applying an irreversible serine peptidase inhibitor to ex vivo skin before exposure to cercariae blocks the majority of larvae from invading, suggesting that this serine peptidase has an essential role in skin penetration .
While the serine peptidase, cercarial elastase, plays a key role in S. mansoni skin invasion, the zoonotic species S. japonicum has no serine peptidases in its larval secretions. S. japonicum, however, encodes a number of isoforms of cathepsin B2 (SjCB2) (GenBank: CAA50305.1), a cysteine peptidase, which are secreted by the invading parasite . Moreover, orthologs of SjCB2 have been identified in the cercarial secretions of other, non-human schistosome species, including members of the genus Trichobilharzia . This led us to the hypothesis that the primary invasive peptidase differs between schistosome species, with S. mansoni, a human-specific schistosome species, utilizing cercarial elastase, and S. japonicum, a zoonotic schistosome species, utilizing cathepsin B2. Given that T. regenti also appears to utilize cathepsin B2 for skin invasion, these observations suggest that the use of a serine peptidase in invasion is the exception, not the rule, among parasitic schistosomes. The use of cercarial elastase may reflect unique properties required by S. mansoni to preferentially infect human hosts.
To confirm that cathepsin B2 is also capable of facilitating skin invasion, we used a proteomic approach to identify potential substrates in host skin, for both S. mansoni cercarial elastase and S. mansoni cathepsin B2 (SmCB2) (GenBank: CAC85211.2), a close homolog of S. japonicum CB2. Although RNAi has been developed as a tool in juvenile and adult schistosome worms, it is currently unavailable for the intramolluscan and cercarial stages of development . We therefore chose to use a proteomic approach to validate the roles of these peptidases in skin invasion.
We found that the vast majority of cleaved proteins resulting from human skin exposure to either purified SmCE or SmCB2 overlap, suggesting that both enzymes are capable of facilitating parasite migration through skin. However, we also identified several potential substrates in skin that appear to be cleaved by only one of the two enzymes. Candidate substrates were further validated by in vitro cleavage of purified human skin proteins with either peptidase. Together, these observations suggest that more than one mechanism of skin penetration may have evolved as an adaptation specific to the schistosome-host relationship.
Phylogenetic analysis of schistosome cercarial elastase and cathepsin B2 proteins
To determine the number of cercarial elastase and cathepsin B2 protein isoforms in schistosome species, all full-length protein sequences (i.e., those possessing the full catalytic core of the peptidase) were collected from both GenBank (NCBI) and S. japonicum and S. mansoni genome annotation websites (Sanger Institute GeneDB). ClustalW (DNA Databank of Japan), was then used to perform multiple sequence alignments and to construct phylogenetic trees. A Blosum protein weight matrix was used to score the alignment, with a gap open penalty of 10, a gap extension penalty of 0.20, and gap distance penalty of 5. Bootstrapping values were calculated using the p-distance method, with a count of 100. The resulting phylogenetic tree was visualized with the program Dendroscope.
Purification of S. mansoni CB2 and CE
S. mansoni cercariae were shed from Biomphalaria glabrata using a light induction method as previously described . SmCE activity was purified from lysate as previously described with the following modifications . Cercariae shed from approximately 50 snails were pelleted by centrifugation at 100 rcf for 1 minute and stored at −20°C. One milliliter of pelleted cercariae was resuspended in 5 ml 300 mM sodium acetate, pH 6.5, 0.1% Triton X-100, 0.1% Tween-20, 0.05% NP40, and sonicated for 1 minute at 40% output. Soluble protein was harvested by centrifugation for 15 minutes at 7, 500 rcf, followed by 0.2 µ filtration. Fractions were again measured for SmCE activity against AAPF-pNA (Ala-Ala-Pro-Phe-p-nitroanilide), and active fractions were run on 10% bis-TRIS polyacrylamide gels (Invitrogen, Carlsbad, CA) according to the manufacturer's specifications, and silver stained . For confirmation of protein identification, bands corresponding to the correct molecular weight of SmCE were excised from the gel, and subjected to in-gel trypsin digestion, followed by LC-MS/MS peptide sequencing, described below. Active site titration was performed using the synthetic peptide inhibitor AAPF-CMK (Ala-Ala-Pro-Phe-chloromethylketone).
Recombinant SmCB2 was expressed in Pichia pastoris as previously described .
Media containing secreted protein underwent 0.2 µ filtration and lyophilization. SmCB2 activity was purified as previously described . Fractions were monitored for SmCB2 activity against 5 µM ZFR-AMC (Z-Phe-Arg-7-amino-4-carbamoylmethylcoumarin) in citrate-phosphate buffer, pH 5.3 supplemented with 4 mM DTT. Enzyme concentration was measured by active site titration using the cysteine peptidase inhibitor CAO74 (N-(L-3-trans-propylcarbamoyloxirane-2- carbonyl)-L-isoleucyl-L-proline).
The human skin sample was taken in compliance with protocols approved by the Committee on Human Research at the University of California, San Francisco. Written informed consent was obtained for the operation and use of tissues removed.
Excised human skin was stored at −80°C. For digestion experiments, skin was thawed, dissected into eight 150–170 mg sections, and placed in 1.5 ml microfuge tubes. To each of these skin sections 100 µl of digestion solution containing either peptidase or inhibited peptidase at 1.8 µM was added, along with corresponding controls. SmCE reaction buffer consisted of 100 mM TRIS-HCl, pH 8; SmCB2 reaction buffer consisted of 100 mM sodium acetate, pH 5.5, 4 mM DTT. Inhibited SmCE was prepared by incubating 1.8 µM SmCE with 2 µM AAPF-CMK for one hour at room temperature; inhibition was monitored against AAPF-pNA, prior to its addition to skin. Similarly, inhibited SmCB2 digestion solution was prepared by incubating 1.8 µM SmCB2 with 2 µM CAO74 for one hour at room temperature, with full inhibition monitored by activity against ZFR-AMC, prior to its addition to skin. Inhibitor alone digestion solutions were prepared to control for human skin peptidase activity using either 2 µM AAPF-CMK in 100 mM Tris, pH 8.0, or 2 µM CAO74 in 100 mM sodium acetate, pH 5.5, 4 mM DTT. After addition of digestion solution to skin samples, the reaction mix was vortexed briefly, and then incubated for 5 hours at 37°C. Following incubation, reactions were centrifuged for 20 minutes at 16,000 rcf at 4°C, and the resulting supernatant was saved as the soluble fraction. Fifteen microliters were removed for analysis on a bis-TRIS 4-20% acrylamide gel. Gels were silver-stained and stored at 4°C.
Proteomic/mass spectrometry analysis
Proteomic analysis of skin digestion samples was performed by LC-MS/MS on two independent preparations as follows. Representative preparative gels are shown in Figures S1 and S2, and contain replicate lanes of approximately 20 µg total protein for each of the skin digestion solutions. Each pair of sample lanes was cut into ten protein bands, and diced into 1–2 mm cubes, then subjected to in-gel trypsin digestion, following a previously published protocol . The resulting peptides were extracted and analyzed by on-line liquid chromatography/mass spectrometry using an Eksigent nanoflow pump and a Famos autosampler that were coupled to a quadrupole-orthogonal-acceleration-time-of-flight hybrid mass spectrometer (QStar Pulsar or QStar Elite, Applied Biosystems, Foster City, CA). Peptides were fractionated on a reversed-phase column (C18, 0.75×150 mm) and a 5–50% B gradient was developed in 35 min at a 350 nl/min flow rate. Solvent A was 0.1% formic acid in water, solvent B was 0.1% formic acid in acetonitrile. Data were acquired in information-dependent acquisition mode: 1 sec MS surveys were followed by 3 sec CID experiments on computer-selected multiply charged precursor ions. Peak lists were generated using Analyst 2.0 software (Applied Biosystems) with the Mascot script 1.6b20 (Matrix Science, London, UK).
Database searches were performed using ProteinProspector v. 5.7.1 (http://prospector2.ucsf.edu) . Searches were performed using the SwissProt databank (August 10, 2010, 519,348 entries). For false discovery rate estimation, this database was concatenated with randomized sequences generated from the original database . Search parameters included selecting trypsin as the digestion enzyme, allowing one missed cleavage but no non-specific cleavages. Peptide modifications that were searched included carbamidomethyl (Cys) as the only fixed modification, and up to two variable modifications from among the following: oxidation (Met), acetyl (N-term), oxidized acetyl (N-term), pyroglutamate (Gln), Met-loss (N-term), and Met-loss+acetyl (N-term). Mass accuracy settings were 200 ppm for precursor and 300 ppm for fragment masses. Data reported in Table S3 has a Protein Prospector minimum score cutoff of 22 (protein), 15 (peptide) and maximum expectation values of 0.01 (protein) and 0.05 (peptide), resulting in a 2% false discovery rate.
Human collagen I and complement C3 cleavage and N-terminal sequencing
Lyophilized type I human skin collagen (Calbiochem) was resuspended in 17.5 mM acetic acid for a final concentration of 1 mg/ml. Human complement C3 (Calbiochem) was purchased as a 1.2 mg/ml stock. For SmCB2 digestion, 180 nM enzyme was added to 50 µl collagen I or 25 µl complement C3 in 50 mM sodium acetate, pH 5.5, 4 mM DTT and incubated at 37°C for 1–22 hours. For SmCE digestion, 180 nM enzyme was added to 50 µl collagen I or 25 µl Complement C3 in 50 mM Tris, pH 8.0 and incubated at 37°C for 1–22 hours. Both enzymes were also pre-incubated with 1 mM CAO74 (SmCB2) or 1 mM AAPF-CMK (SmCE) for one hour at room temperature prior to their addition to collagen. As a control, collagen was incubated in 50 mM sodium acetate, pH 5.5, 4 mM DTT or 50 mM Tris, pH 8.0 for 22 hours at 37°C. To stop the reaction, 15 µl reduced SDS-PAGE loading dye (Invitrogen) was added, and a sample of each reaction was run on a 4–20% Tris-Glycine SDS PAGE gel (Invitrogen). Bands were then electroblotted onto PVDF membrane (Biorad, Foster City, CA) and visualized by Coomassie Blue staining. N-terminal sequence of selected bands was determined using Edman chemistry on an Applied Biosystems Procise liquid-pulse protein sequenator at the Protein and Nucleotide Facility, Stanford University.
Identification and phylogeny of cercarial elastase and cathepsin B2 isoforms
To outline the molecular evolution of larval peptidases in schistosomes, all previously reported orthologs were re-examined (Figure 1). In addition to the previously identified full-length cercarial elastase isoforms in S. mansoni--SmCE1a (GenBank: AAM43939.1), SmCE1b (GenBank: CAA94312.1), SmCE1c (GenBank: AAC46968.1), SmCE2a (AAM43941.1) and SmCE2b (GenBank: AAM43942.1) and Schistosoma haematobium cercarial elastase (GenBank: AAM4394)--sequencing and annotation of the full S. mansoni genome revealed three additional full-length genes ,  (Figure 1A). In marked contrast, the S. japonicum genome contains only a single cercarial elastase isoform (Sjp_0028090). No cercarial elastase genes have been detected in any Trichobilharzia species.
Phylogenetic analysis of cercarial elastase (A) and cathepsin B2 (B) protein sequences reveals expansion of each gene family in different lineages of schistosomes. Boxes indicate proteins previously determined to be present in cercarial secretions, as determined by LC MS/MS.
Both S. mansoni and S. japonicum encode a number of cathepsin B genes (Figure S3). We chose to focus on the cathepsin B2 isotype, since a proteomic analysis of S. japonicum cercarial secretions identified a peptide sequence common to this subset  (Figure 1B). Notably, while the S. mansoni genome encodes only a single cathepsin B2 isoform, S. japonicum encodes four CB2 isoforms. In one of these isoforms, SjCB(Y)2d (GenBank: CAX71091.1), the nucleophilic cysteine of the active site is mutated to tyrosine, which may diminish, if not eliminate, its catalytic activity. Three of the four SjCB2 isoforms (SjCB2b (GenBank: CAX71088.1), SjCB2c (GenBank: CAX71090.1) and SjCB(Y)2d correspond to the peptide sequence identified in proteomic analysis of S. japonicum cercarial secretions . A full list of schistosome cercarial elastase and cathepsin B isoforms is provided as supplementary material (Tables S1 and S2).
Comparative proteomic analysis of human skin treated with SmCE and SmCB2
A previous proteomic study generated a list of proteins that were released as soluble peptides from ex vivo human skin upon treatment with live S. mansoni cercariae, indicating that they are actively degraded during cercarial migration through skin . These included many of the structural components of skin, including extracellular matrix proteins, proteins involved in cell-cell adhesion and multiple serum proteins. To identify specific substrates of CE in skin, and to compare these to potential substrates of cathepsin B2, we treated ex vivo skin with either peptidase. Since active, recombinant S. japonicum cathepsin B2 is not currently available, and purifying sufficient amounts of native peptidase from S. japonicum was not feasible, we used S. mansoni cathepsin B2 as model peptidase in our analysis. S. mansoni cathepsin B2 has high homology to the S. japonicum cathepsin B2 (90% sequence identity and 94% sequence similarity for the mature peptidase, see Figure S4), including the active site and substrate binding pocket, and therefore is likely to display highly similar biochemical properties and substrate specificity . SmCE was purified directly from S. mansoni cercariae, and the protein composition of proteolytically active fractions was determined by mass spectrometric analysis as a mixture of SmCE1a, 1b and 2a isoforms, but not SmCE2b. This is consistent with the isoform composition of previous proteomic analysis of S. mansoni cercarial secretions , . Active SmCB2 was expressed in recombinant form in P. pastoris and purified as previously described . To ensure that equimolar amounts of active enzyme were added to skin samples, an active site titration was first performed for both SmCE and SmCB2 with respective covalent inhibitors.
In comparison to control samples treated with inhibited peptidase, multiple skin proteins migrated through an SDS-PAGE gel as smaller fragments, i.e. fragments less than the predicted molecular weight of the full-length protein, upon addition of active SmCE or SmCB2. These were thus identified as substrates of the specific enzyme and included multiple extracellular matrix proteins (Table 1). Addition of both SmCE and SmCB2 to skin led to the cleavage of collagen VI, which is found in interstitial tissue, and collagen XII, a collagen located in the basement membrane of the epidermis . Only samples incubated with active SmCB2 showed cleavage of collagens I, III and XVIII. In addition to collagen, several other components of the extracellular matrix were degraded upon treatment with either peptidase, including vitronectin, fibronectin, and galectin. Both vimentin and talin-1, cytoskeletal proteins that are associated with desmosomes, were cleaved upon addition of either peptidase. Two additional extracellular matrix components, tenascin-X and thrombospondin-1, were uniquely cleaved upon addition of SmCB2.
Another subset of extracellular proteins identified as substrates of SmCE and SmCB2 were derived from blood plasma that bathes the dermis. These included components of the coagulation cascade, e. g. fibrinogen, antithrombin-III, as well as proteins involved in the host immune response, e. g. complement C3, complement factor D. Addition of either active SmCE or SmCB2 led to the digestion of gelsolin, an actin assembly protein that exists intracellularly and in plasma. Addition of active SmCB2 also led to the digestion of both kininogen-1 and fibrinogen, both of which are members of the coagulation cascade. Complement C3, an integral component of both the classical and alternative complement activation pathways was cleaved upon addition of either SmCE and SmCB2; complement C4A and complement D proteins, respective members of the classical and alternative complement activation pathways, were cleaved by SmCB2 alone.
In addition to the extracellular proteins identified, many cytosolic proteins were also cleaved by either SmCE or SmCB2. A complete list of peptides identified is provided as a supplementary table (Table S3).
In vitro digestion of human collagen I and complement C3
To corroborate proteomic identification of substrates in skin, candidate substrates were selected for in vitro digestion with either SmCE or SmCB2. Type I collagen was of particular interest, given that lower molecular weight peptides of the protein were only found in skin samples treated with SmCB2, suggesting it is cleaved by SmCB2 but not SmCE. To test this with purified protein, type I human collagen was treated with either SmCB2 or SmCE for up to 22 hours at 37°C, and cleavage of the protein was determined by SDS-PAGE analysis (Figure 2). While the majority of collagen I was degraded after 5 hours with SmCB2 (Figure 2A), SmCE treatment resulted in the appearance of discrete lower molecular weight bands only after 22 hours of enzyme treatment (Figure 2B). This confirms that SmCE shows reduced activity against type I collagen relative to SmCB2, even in vitro. To confirm that the two peptidases cleaved collagen at unique sites, candidate lower molecular weight bands resulting from peptidase treatment were submitted for N-terminal sequencing, and the resulting amino acid sequence was mapped onto the full protein to determine cleavage sites (Figure 2C). Consistent with previous analysis of SmCE substrate specificity, in vitro digestion of collagen I revealed that peptide bond cleavage only occurred following a leucine residue (VRGL/TGPI) , . In comparison, SmCB2 cleavage occurred following an arginine residue (GER/GGP), which is consistent with its reported activity, including a level of “promiscuity” in its amino acid preference in the P2 substrate binding pocket, relative to other types of cathepsins , .
In vitro cleavage of human collagen I confirms ex vivo analysis, and reveals differential cleavage by CE and CB2. (A) SmCB2 digestion of collagen I (B) SmCE digestion of collagen I. Digestion reactions were performed for 1–22 hours at 37°C. * indicates pre-incubation with (A) 1 mM CAO74 or (B) 1 mM AAPF-CMK. ** indicates no peptidase control. Arrows indicate bands submitted for Edman degradation. (C) Schematic of human collagen I, indicating cleavage sites as determined by Edman degradation (VWC-Von Willenbrand Factor Type C domain; COL- collagen triple helix repeat).
Complement C3 was also of particular interest as a potential substrate of both SmCE and SmCB2, given its role in the host immune response against the parasite . Purified complement C3 was treated with SmCB2 or SmCE. Discrete lower molecular weight bands were visible within 1 hour of treatment with either peptidase, in comparison to inhibited peptidase controls (Figure 3A, B). N-terminal sequencing of selected fragments again revealed that both SmCE and SmCB2 digested the protein in a manner consistent with their known specificities, with an arginine in the P1 position (RR/SVQ) for SmCB2 and and a tyrosine in the P1 position (TMY/HAK) for SmCE (Figure 3C).
In vitro cleavage of human complement C3 protein confirms ex vivo analysis, and reveals differential cleavage by CE and CB2. (A) SmCB2 digestion of complement C3. (B) SmCE digestion of complement C3. Digestion reactions were performed for 1–22 hours (SmCB2) or 1–5 hours (SmCE) at 37°C. * indicates pre-incubation with (A) 1 mM CAO74 or (B) 1 mM AAPF-CMK. ** indicates no peptidase control. Arrows indicate bands submitted for Edman degradation. (C) Schematic of human complement C3, indicating cleavage sites as determined by Edman degradation (MGI-macroglobulin-1; A2M-alpha-2-macroglobulin; ANA-.Anaphylatoxin homologous domain; NTR-netrin-like domain).
In S. mansoni, the most abundant peptidase in cercarial secretions is a serine peptidase, termed cercarial elastase (SmCE) for its ability to degrade insoluble elastin , . In addition to proteomic analysis, biochemical and immunolocalization studies have detected SmCE activity in cercarial secretions and confirmed that the enzyme is able to cleave such substrates as type IV collagen (basement membrane collagen), fibronectin, laminin and immunoglobulin in vitro , , . Here, we have shown that SmCE cleaves additional substrates in skin, including several types of collagen, other extracellular matrix proteins, and components of the complement cascade.
Recent sequencing and annotation of the S. mansoni genome suggests a unique role for cercarial elastase. An expanded gene family was identified with ten individual genes that encode multiple isoforms of the peptidase. Even without a complete genome, multiple orthologs of SmCE have been also been found in S. haematobium, a related human-specific species of schistosome common throughout North Africa and the Middle East . This is not the case for the zoonotic S. japonicum, a schistosome species that infects humans and other mammals throughout southeast Asia. The S. japonicum genome contains only a single gene encoding cercarial elastase. This gene corresponds to the cercarial elastase “2b” isoform in S. mansoni, for which minimal transcript is made relative to other CE isoforms (Ingram and McKerrow, unpublished). While one report suggested that CE was detected by immunofluorescence in S. japonicum secretions, no cercarial elastase protein was detected in a high resolution mass spectrometric proteomic analysis of S. japonicum acetabular secretions, and no cercarial elastase-like activity was identified by direct biochemical assays , . Trichobilharzia regenti, an avian schistosome that is capable of invading human skin, but not establishing a successful infection in humans, encodes a cysteine peptidase, cathepsin B2 (TrCB2 (GenBank: ABS57370.1)), which has elastinolytic properties and localizes to the acetabular glands of the parasite . S. japonicum also encodes a cathepsin B2 ortholog, and transcript is expressed in the developing larval stage of the parasite. Moreover, proteomic analysis has identified cathepsin B2 as being present in S. japonicum cercarial secretions . Notably, S. japonicum has 40- fold higher cathepsin B activity in its acetabular secretions, relative to S. mansoni secretions . It is therefore likely that in S. japonicum cercariae, cathepsin B2, not cercarial elastase, is the predominant invasive enzyme.
The differential use of these two classes of peptidases raises the question of how their respective pH optima are achieved in schistosome secretions. SmCB2 is maximally active under acidic, reducing conditions . Since the influence of S. japonicum cercarial secretions on the local environment of skin is unknown, SmCB2 incubations were performed under acidic conditions to ensure optimal peptidase activity. SmCE activity is optimal in a slightly alkaline environment, and S. mansoni secretions are also alkaline; therefore all SmCE incubations were performed at pH 8 . Certainly, for S. mansoni, the evolutionary selection is most likely coordination of the pH of the acetabular gland secretions and the pH optimum of the peptidase. The pH optimum of the cercarial elastase is 8, and the pH of the secretions is also alkaline . As S. mansoni cercariae migrate through skin, a microenvironment is created by the secreted material, which allows for optimal activity of the peptidase. The situation is less clear for S. japonicum and the Trichobilharzia cercariae. While some activity of the cathepsin B2 is likely to continue at neutral, or even alkaline pH, the pH optimum is slightly acidic . The situation is reminiscent of the secretion of cathepsin B by macrophages into tissue compartments of vertebrates. Secreted human cathepsin B is known to degrade extracellular matrix proteins in human tissue, where it has been reported to facilitate tumor invasion and metastasis . The pH optimum of mammalian cathepsin B is also slightly acidic . It is not known if the microenvironment around migrating macrophages is acidic or when that enzyme is released; however, it appears that there is sufficient cathepsin B activity to cause tissue degradation.
Given the unavailability of active, recombinant SjCB2 or sufficient amounts of S. japonicum cercariae from which to purify the native enzyme, we chose to perform our proteomic study with SmCB2, which displays high sequence homology (90% amino acid sequence identity for the mature peptidase) to its S. japonicum ortholog. We therefore hypothesized that it is likely to display similar biochemical characteristics, including similar substrate specificity. While we cannot say conclusively that SjCB2 is the protease facilitating S. japonicum cercarial invasion, we believe that our study, along with previous work from other groups, supports the proposed role for cathepsin B2 in host skin protein degradation , .
This conclusion, that S. japonicum uses a cathepsin B2 peptidase for skin invasion, while S. mansoni uses a serine peptidase (SmCE), has implications for the evolution of the human host-parasite relationship in schistosomiasis. A plausible model is that the cathepsin B2 family first emerged as the functional cercarial peptidase during trematode evolution. In contrast, the “humanized” parasites such as S. mansoni appear to have switched to a serine peptidase for cercarial invasion. This model is supported by the notable expansion of the serine peptidase gene family from the single 2b gene found in S. japonicum to the multiple isoforms expressed in S. mansoni , . While the genome of the other “humanized” parasite, S. haematobium, has not been completed, it is already clear from EST analysis that more abundant serine peptidase isoforms are present in that genome .
What is the advantage of a larval serine peptidase for the “humanized” schistosomes? It is interesting to note that by BLAST analysis, some of the proteins with highest homology to cercarial elastase are mammalian mast cell peptidases, which are present in skin . It is therefore possible that cercarial elastase evolved by convergence to resemble a human peptidase, in order to evade detection by the host immune system. Previous work shows that S. mansoni cercariae migrate through skin at a much slower rate than their S. japonicum counterparts . Despite this, an inflammatory response to S. japonicum cercariae occurs more frequently than to S. mansoni cercariae , . Cathepsin B2 is a likely target of the inflammatory response, given that many cysteine peptidases are allergenic . Perhaps the rapid transit of non-humanized cercariae through skin precludes the need for an invasive enzyme that mimics a host peptidase. Other aspects of immune evasion, such as the elimination of complement factors and immunoglobulin, may be common to both species. C3 and C4 components bind to the tegument of schistosomes, but are degraded by both SmCE and SmCB2 , .
The results reported here show that S. mansoni cathepsin B2 (a model for S. japonicum cathepsin B2) and S. mansoni cercarial elastase are both capable of degrading proteins in skin that act as a barrier to cercarial invasion. Many skin proteins are substrates for both enzymes, but cathepsin B2 appears to cleave a broader range of substrates, and therefore may be a more effective invasive enzyme than cercarial elastase.
Preparative and analytical SmCE SDS-PAGE gels. (A) Preparative SDS-PAGE gel with duplicate lanes loaded with skin lysate, treated with (1) 180 nM SmCE, (2) 1.8 µM SmCE, (3) 2 µM AAPF-CMK followed by 2 µM SmCE, or (4) no enzyme. (B) The same samples loaded at the analytical scale, at 1/10 concentration compared to (A).
Preparative and analytical SmCB2 SDS-PAGE gels. (A) Preparative SDS-PAGE gel with duplicate lanes loaded with skin lysate, treated with (1) 180 nM SmCB2, (2) 1.8 µM SmCB2, (3) 1.8 µM CA074+1.8 µM SmCB2, or (4) 1.8 uM CA074. (B) The same samples loaded at the analytical scale, at 1/10 concentration compared to (A).
Expanded phylogenetic analysis of all known schistosome cathepsin B proteins.
SmCB2 and SjCB2 protein alignment. ClustalW alignment of SmCB2 (GenBank: CAZ31207.1) and SjCB2 (GenBank: AAO59414.2) protein sequences shows high sequence identity.
Complete list of schistosome cathepsin B sequences. All available schistosome cathepsin B isoforms from S. mansoni and S. japonicum were compiled from GenBank and their respective annotation websites. For each, the name used for this study, previous identifiers, and the full amino acid sequence are listed. Active site residues are highlighted in red. S. mansoni sequences are highlighted in pink, and S. japonicum sequences are highlighted in blue.
Complete list of schistosome cercarial elastase sequences. All available full-length sequences (i. e. those possessing the full catalytic core) cercarial elastase sequence from S. mansoni, S. haematobium and S. japonicum were compiled from GenBank and their respective annotation websites. For each, the name used for this study, previous identifiers, and the full amino acid sequence are listed. Active site residues are highlighted in red. S. mansoni sequences are highlighted in pink, S. haematobium seqeuences are highlighted in purple and S. japonicum sequences are highlighted in blue.
Complete list of proteins identified in proteomic analysis of skin treated with schistosome peptidases. Two replicate experiments are shown. For each protein, the accession number, number of unique peptides, percent coverage, and respective scores are listed (detail of how scores were calculated is described in Methods), along with species name and protein name. Samples were compared based on the number of unique peptides for each experimental condition.
The authors wish to thank Dr. Fred Lewis of the NIH-NIAID Schistosomiasis Resource center for providing infected Biomphalaria; Stephanie Hopkins, for providing the scanning EM image of an S. mansoni cercaria; Conor Caffrey and Debbie Ruelas, for providing recombinant SmCB2; and the members of the McKerrow and Craik laboratories at UCSF for providing helpful discussion.
Conceived and designed the experiments: JI GK JS JM. Performed the experiments: JI GK. Analyzed the data: JI GK JS. Contributed reagents/materials/analysis tools: KCL EH. Wrote the paper: JI JM.
- 1. Elias PM (1996) Stratum corneum architecture, metabolic activity and interactivity with subjacent cell layers. Exp Dermatol 5: 191–201.PM Elias1996Stratum corneum architecture, metabolic activity and interactivity with subjacent cell layers.Exp Dermatol5191201
- 2. Fukuyama K, Tzeng S, McKerrow J, Epstein WL (1983) The epidermal barrier to Schistosoma mansoni infection. Curr Probl Dermatol 11: 185–193.K. FukuyamaS. TzengJ. McKerrowWL Epstein1983The epidermal barrier to Schistosoma mansoni infection.Curr Probl Dermatol11185193
- 3. Stirewalt MA, Dorsey CH (1974) Schistosoma mansoni: cercarial penetration of host epidermis at the ultrastructural level. Exp Parasitol 35: 1–15.MA StirewaltCH Dorsey1974Schistosoma mansoni: cercarial penetration of host epidermis at the ultrastructural level.Exp Parasitol35115
- 4. Mair GR, Maule AG, Fried B, Day TA, Halton DW (2003) Organization of the musculature of schistosome cercariae. J Parasitol 89: 623–625.GR MairAG MauleB. FriedTA DayDW Halton2003Organization of the musculature of schistosome cercariae.J Parasitol89623625
- 5. Haas W, Grabe K, Geis C, Pach T, Stoll K, et al. (2002) Recognition and invasion of human skin by Schistosoma mansoni cercariae: the key-role of L-arginine. Parasitology 124: 153–167.W. HaasK. GrabeC. GeisT. PachK. Stoll2002Recognition and invasion of human skin by Schistosoma mansoni cercariae: the key-role of L-arginine.Parasitology124153167
- 6. Knudsen GM, Medzihradszky KF, Lim KC, Hansell E, McKerrow JH (2005) Proteomic analysis of Schistosoma mansoni cercarial secretions. Mol Cell Proteomics 4: 1862–1875.GM KnudsenKF MedzihradszkyKC LimE. HansellJH McKerrow2005Proteomic analysis of Schistosoma mansoni cercarial secretions.Mol Cell Proteomics418621875
- 7. Curwen RS, Ashton PD, Johnston DA, Wilson RA (2004) The Schistosoma mansoni soluble proteome: a comparison across four life-cycle stages. Mol Biochem Parasitol 138: 57–66.RS CurwenPD AshtonDA JohnstonRA Wilson2004The Schistosoma mansoni soluble proteome: a comparison across four life-cycle stages.Mol Biochem Parasitol1385766
- 8. McKerrow JH, Keene WE, Jeong KH, Werb Z (1983) Degradation of extracellular matrix by larvae of Schistosoma mansoni. I. Degradation by cercariae as a model for initial parasite invasion of host. Lab Invest 49: 195–200.JH McKerrowWE KeeneKH JeongZ. Werb1983Degradation of extracellular matrix by larvae of Schistosoma mansoni. I. Degradation by cercariae as a model for initial parasite invasion of host.Lab Invest49195200
- 9. McKerrow JH, Pino-Heiss S, Lindquist R, Werb Z (1985) Purification and characterization of an elastinolytic proteinase secreted by cercariae of Schistosoma mansoni. J Biol Chem 260: 3703–3707.JH McKerrowS. Pino-HeissR. LindquistZ. Werb1985Purification and characterization of an elastinolytic proteinase secreted by cercariae of Schistosoma mansoni.J Biol Chem26037033707
- 10. Salter JP, Lim KC, Hansell E, Hsieh I, McKerrow JH (2000) Schistosome invasion of human skin and degradation of dermal elastin are mediated by a single serine protease. J Biol Chem 275: 38667–38673.JP SalterKC LimE. HansellI. HsiehJH McKerrow2000Schistosome invasion of human skin and degradation of dermal elastin are mediated by a single serine protease.J Biol Chem2753866738673
- 11. Lim KC, Sun E, Bahgat M, Bucks D, Guy R, et al. (1999) Blockage of skin invasion by schistosome cercariae by serine protease inhibitors. Am J Trop Med Hyg 60: 487–492.KC LimE. SunM. BahgatD. BucksR. Guy1999Blockage of skin invasion by schistosome cercariae by serine protease inhibitors.Am J Trop Med Hyg60487492
- 12. Dvorak J, Mashiyama ST, Braschi S, Sajid M, Knudsen GM, et al. (2008) Differential use of protease families for invasion by schistosome cercariae. Biochimie 90: 345–358.J. DvorakST MashiyamaS. BraschiM. SajidGM Knudsen2008Differential use of protease families for invasion by schistosome cercariae.Biochimie90345358
- 13. Doleckova K, Kasny M, Mikes L, Cartwright J, Jedelsky P, et al. (2009) The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti. Int J Parasitol 39: 201–211.K. DoleckovaM. KasnyL. MikesJ. CartwrightP. Jedelsky2009The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti.Int J Parasitol39201211
- 14. Stefanic S, Dvorak J, Horn M, Braschi S, Sojka D, et al. (2010) RNA interference in Schistosoma mansoni schistosomula: selectivity, sensitivity and operation for larger-scale screening. PLoS Negl Trop Dis 4: e850.S. StefanicJ. DvorakM. HornS. BraschiD. Sojka2010RNA interference in Schistosoma mansoni schistosomula: selectivity, sensitivity and operation for larger-scale screening.PLoS Negl Trop Dis4e850
- 15. Salter JP, Choe Y, Albrecht H, Franklin C, Lim KC, et al. (2002) Cercarial elastase is encoded by a functionally conserved gene family across multiple species of schistosomes. J Biol Chem 277: 24618–24624.JP SalterY. ChoeH. AlbrechtC. FranklinKC Lim2002Cercarial elastase is encoded by a functionally conserved gene family across multiple species of schistosomes.J Biol Chem2772461824624
- 16. Mortz E, Krogh TN, Vorum H, Gorg A (2001) Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis. Proteomics 1: 1359–1363.E. MortzTN KroghH. VorumA. Gorg2001Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis.Proteomics113591363
- 17. Caffrey CR, Salter JP, Lucas KD, Khiem D, Hsieh I, et al. (2002) SmCB2, a novel tegumental cathepsin B from adult Schistosoma mansoni. Mol Biochem Parasitol 121: 49–61.CR CaffreyJP SalterKD LucasD. KhiemI. Hsieh2002SmCB2, a novel tegumental cathepsin B from adult Schistosoma mansoni.Mol Biochem Parasitol1214961
- 18. Chalkley RJ, Baker PR, Hansen KC, Medzihradszky KF, Allen NP, et al. (2005) Comprehensive analysis of a multidimensional liquid chromatography mass spectrometry dataset acquired on a quadrupole selecting, quadrupole collision cell, time-of-flight mass spectrometer - I. How much of the data is theoretically interpretable by search engines? Molecular & Cellular Proteomics 4: 1189–1193.RJ ChalkleyPR BakerKC HansenKF MedzihradszkyNP Allen2005Comprehensive analysis of a multidimensional liquid chromatography mass spectrometry dataset acquired on a quadrupole selecting, quadrupole collision cell, time-of-flight mass spectrometer - I. How much of the data is theoretically interpretable by search engines?Molecular & Cellular Proteomics411891193
- 19. Elias JE, Gygi SP (2007) Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry. Nature Methods 4: 207–214.JE EliasSP Gygi2007Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry.Nature Methods4207214
- 20. Consortium (2009) The Schistosoma japonicum genome reveals features of host-parasite interplay. Nature 460: 345–351.Consortium2009The Schistosoma japonicum genome reveals features of host-parasite interplay.Nature460345351
- 21. Hansell E, Braschi S, Medzihradszky KF, Sajid M, Debnath M, et al. (2008) Proteomic analysis of skin invasion by blood fluke larvae. PLoS Negl Trop Dis 2: e262.E. HansellS. BraschiKF MedzihradszkyM. SajidM. Debnath2008Proteomic analysis of skin invasion by blood fluke larvae.PLoS Negl Trop Dis2e262
- 22. Curwen RS, Ashton PD, Sundaralingam S, Wilson RA (2006) Identification of novel proteases and immunomodulators in the secretions of schistosome cercariae that facilitate host entry. Mol Cell Proteomics 5: 835–844.RS CurwenPD AshtonS. SundaralingamRA Wilson2006Identification of novel proteases and immunomodulators in the secretions of schistosome cercariae that facilitate host entry.Mol Cell Proteomics5835844
- 23. Lunstrum GP, Morris NP, McDonough AM, Keene DR, Burgeson RE (1991) Identification and partial characterization of two type XII-like collagen molecules. J Cell Biol 113: 963–969.GP LunstrumNP MorrisAM McDonoughDR KeeneRE Burgeson1991Identification and partial characterization of two type XII-like collagen molecules.J Cell Biol113963969
- 24. Cohen FE, Gregoret LM, Amiri P, Aldape K, Railey J, et al. (1991) Arresting tissue invasion of a parasite by protease inhibitors chosen with the aid of computer modeling. Biochemistry 30: 11221–11229.FE CohenLM GregoretP. AmiriK. AldapeJ. Railey1991Arresting tissue invasion of a parasite by protease inhibitors chosen with the aid of computer modeling.Biochemistry301122111229
- 25. Choe Y, Leonetti F, Greenbaum DC, Lecaille F, Bogyo M, et al. (2006) Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities. J Biol Chem 281: 12824–12832.Y. ChoeF. LeonettiDC GreenbaumF. LecailleM. Bogyo2006Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities.J Biol Chem2811282412832
- 26. Castro-Borges W, Dowle A, Curwen RS, Thomas-Oates J, Wilson RA (2011) Enzymatic shaving of the tegument surface of live schistosomes for proteomic analysis: a rational approach to select vaccine candidates. PLoS Negl Trop Dis 5: e993.W. Castro-BorgesA. DowleRS CurwenJ. Thomas-OatesRA Wilson2011Enzymatic shaving of the tegument surface of live schistosomes for proteomic analysis: a rational approach to select vaccine candidates.PLoS Negl Trop Dis5e993
- 27. Aslam A, Quinn P, McIntosh RS, Shi J, Ghumra A, et al. (2008) Proteases from Schistosoma mansoni cercariae cleave IgE at solvent exposed interdomain regions. Mol Immunol 45: 567–574.A. AslamP. QuinnRS McIntoshJ. ShiA. Ghumra2008Proteases from Schistosoma mansoni cercariae cleave IgE at solvent exposed interdomain regions.Mol Immunol45567574
- 28. Sloane BF, Honn KV (1984) Cysteine proteinases and metastasis. Cancer Metastasis Rev 3: 249–263.BF SloaneKV Honn1984Cysteine proteinases and metastasis.Cancer Metastasis Rev3249263
- 29. Fishelson Z, Amiri P, Friend DS, Marikovsky M, Petitt M, et al. (1992) Schistosoma mansoni: cell-specific expression and secretion of a serine protease during development of cercariae. Exp Parasitol 75: 87–98.Z. FishelsonP. AmiriDS FriendM. MarikovskyM. Petitt1992Schistosoma mansoni: cell-specific expression and secretion of a serine protease during development of cercariae.Exp Parasitol758798
- 30. McKerrow JH, Salter J (2002) Invasion of skin by Schistosoma cercariae. Trends Parasitol 18: 193–195.JH McKerrowJ. Salter2002Invasion of skin by Schistosoma cercariae.Trends Parasitol18193195
- 31. Cavallo-Medved D, Rudy D, Blum G, Bogyo M, Caglic D, et al. (2009) Live-cell imaging demonstrates extracellular matrix degradation in association with active cathepsin B in caveolae of endothelial cells during tube formation. Exp Cell Res 315: 1234–1246.D. Cavallo-MedvedD. RudyG. BlumM. BogyoD. Caglic2009Live-cell imaging demonstrates extracellular matrix degradation in association with active cathepsin B in caveolae of endothelial cells during tube formation.Exp Cell Res31512341246
- 32. Werle B, Ebert W, Klein W, Spiess E (1994) Cathepsin B in tumors, normal tissue and isolated cells from the human lung. Anticancer Res 14: 1169–1176.B. WerleW. EbertW. KleinE. Spiess1994Cathepsin B in tumors, normal tissue and isolated cells from the human lung.Anticancer Res1411691176
- 33. Berriman M, Haas BJ, LoVerde PT, Wilson RA, Dillon GP, et al. (2009) The genome of the blood fluke Schistosoma mansoni. Nature 460: 352–358.M. BerrimanBJ HaasPT LoVerdeRA WilsonGP Dillon2009The genome of the blood fluke Schistosoma mansoni.Nature460352358
- 34. He YX, Salafsky B, Ramaswamy K (2005) Comparison of skin invasion among three major species of Schistosoma. Trends Parasitol 21: 201–203.YX HeB. SalafskyK. Ramaswamy2005Comparison of skin invasion among three major species of Schistosoma.Trends Parasitol21201203
- 35. Jenkins SJ, Hewitson JP, Jenkins GR, Mountford AP (2005) Modulation of the host's immune response by schistosome larvae. Parasite Immunol 27: 385–393.SJ JenkinsJP HewitsonGR JenkinsAP Mountford2005Modulation of the host's immune response by schistosome larvae.Parasite Immunol27385393
- 36. Kikuchi Y, Takai T, Kuhara T, Ota M, Kato T, et al. (2006) Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p1 to sensitization toward IgE and IgG responses. J Immunol 177: 1609–1617.Y. KikuchiT. TakaiT. KuharaM. OtaT. Kato2006Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p1 to sensitization toward IgE and IgG responses.J Immunol17716091617
- 37. Ruppel A, McLaren DJ, Diesfeld HJ, Rother U (1984) Schistosoma mansoni: escape from complement-mediated parasiticidal mechanisms following percutaneous primary infection. Eur J Immunol 14: 702–708.A. RuppelDJ McLarenHJ DiesfeldU. Rother1984Schistosoma mansoni: escape from complement-mediated parasiticidal mechanisms following percutaneous primary infection.Eur J Immunol14702708