RNA Interference in
Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening
The possible emergence of resistance to the only available drug for schistosomiasis spurs drug discovery that has been recently incentivized by the availability of improved transcriptome and genome sequence information. Transient RNAi has emerged as a straightforward and important technique to interrogate that information through decreased or loss of gene function and identify potential drug targets. To date, RNAi studies in schistosome stages infecting humans have focused on single (or up to 3) genes of interest. Therefore, in the context of standardizing larger RNAi screens, data are limited on the extent of possible off-targeting effects, gene-to-gene variability in RNAi efficiency and the operational capabilities and limits of RNAi.
We investigated in vitro the sensitivity and selectivity of RNAi using double-stranded (ds)RNA (approximately 500 bp) designed to target 11 Schistosoma mansoni genes that are expressed in different tissues; the gut, tegument and otherwise. Among the genes investigated were 5 that had been previously predicted to be essential for parasite survival. We employed mechanically transformed schistosomula that are relevant to parasitism in humans, amenable to screen automation and easier to obtain in greater numbers than adult parasites. The operational parameters investigated included defined culture media for optimal parasite maintenance, transfection strategy, time- and dose- dependency of RNAi, and dosing limits. Of 7 defined culture media tested, Basch Medium 169 was optimal for parasite maintenance. RNAi was best achieved by co-incubating parasites and dsRNA (standardized to 30 µg/ml for 6 days); electroporation provided no added benefit. RNAi, including interference of more than one transcript, was selective to the gene target(s) within the pools of transcripts representative of each tissue. Concentrations of dsRNA above 90 µg/ml were directly toxic. RNAi efficiency was transcript-dependent (from 40 to >75% knockdown relative to controls) and this may have contributed to the lack of obvious phenotypes observed, even after prolonged incubations of 3 weeks. Within minutes of their mechanical preparation from cercariae, schistosomula accumulated fluorescent macromolecules in the gut indicating that the gut is an important route through which RNAi is expedited in the developing parasite.
Transient RNAi operates gene-selectively in S. mansoni newly transformed schistosomula yet the sensitivity of individual gene targets varies. These findings and the operational parameters defined will facilitate larger RNAi screens.
RNA interference (RNAi) is a technique to selectively suppress mRNA of individual genes and, consequently, their cognate proteins. RNAi using double-stranded (ds) RNA has been used to interrogate the function of mainly single genes in the flatworm, Schistosoma mansoni, one of a number of schistosome species causing schistosomiasis. In consideration of large-scale screens to identify candidate drug targets, we examined the selectivity and sensitivity (the degree of suppression) of RNAi for 11 genes produced in different tissues of the parasite: the gut, tegument (surface) and otherwise. We used the schistosomulum stage prepared from infective cercariae larvae which are accessible in large numbers and adaptable to automated screening platforms. We found that RNAi suppresses transcripts selectively, however, the sensitivity of suppression varies (40%–>75%). No obvious changes in the parasite occurred post-RNAi, including after targeting the mRNA of genes that had been computationally predicted to be essential for survival. Additionally, we defined operational parameters to facilitate large-scale RNAi, including choice of culture medium, transfection strategy to deliver dsRNA, dose- and time-dependency, and dosing limits. Finally, using fluorescent probes, we show that the developing gut allows rapid entrance of dsRNA into the parasite to initiate RNAi.
Citation: Štefanić S, Dvořák J, Horn M, Braschi S, Sojka D, Ruelas DS, et al. (2010) RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening. PLoS Negl Trop Dis 4(10): e850. doi:10.1371/journal.pntd.0000850
Editor: Malcolm K. Jones, University of Queensland, Australia
Received: June 25, 2010; Accepted: September 16, 2010; Published: October 19, 2010
Copyright: © 2010 Štefanić et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The research was supported by the Sandler Foundation and, in part, by a Collaborative Research Project grant to CRC (WHO T24/181/101, ID # A50567) from the United Nations Children's Fund/United Nations Development Programme/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases (TDR). JD was supported by a FP7Marie Curie - International Re-integration Grant (248642) and by a Czech Ministry of Education, Youth and Sports (MÅ MT) grant (ME1001) awarded under the auspices of the American Science Information Center (AMVIS). SS was supported by a Prospective Scientists Fellowship (PBZHA-121000) from the Swiss National Science Foundation. MH was supported by a grant (203/09/1585) from the Grant Agency of the Czech Republic (GACR), a research project grant Z40550506 and by the Academy of Science of the Czech Republic. DS was supported by a Visiting Scholar Fulbright award (2008-21-06), a grant from the GACR (KJB600960911) and by the Research Center No. LC06009 from the MÅ MT. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Schistosomiasis is one of a number of ‘neglected tropical diseases’ (NTDs) associated with extreme poverty in the world's ‘bottom billion’ people . Caused by a parasitic flatworm, the disease can be fatal, but is better known for its chronicity of infection (e.g., ). Clinical manifestations of chronic infection include malaise, anemia and wasting, and poor cognitive performance in children , , , , . Infection with Schistosoma haematobium is associated with an increased risk of bladder cancer ,  and due to the compromised integrity of the lower female genital tract, with HIV infection , , .
Because treatment and control of schistosomiasis has come to rely on a single drug, praziquantel (PZQ; , , ), concern remains over the possible emergence and establishment of clinically relevant drug resistance . There are reports of decreased, if sometimes, transient , susceptibility by field-isolates of parasite to PZQ . The risk of resistance to PZQ ,  is all the more relevant given the impetus to increase access to this and other anthelmintics via integrated mass administration programs , , , , . Thus, it remains a priority to identify and develop alternative chemo- and immuno-therapeutic interventions for schistosomiasis, and progress is being made in both these areas , , , , .
Key initial tasks for drug development are the identification and validation of gene products for which modulation by chemical and/or genetic means translates to impaired cell/organism survival. For schistosomes, comprehensive transcriptome , , , , , , , ,  and genome sequence information , ,  is now accessible through interrogable databases , , , . These databases have facilitated new strategies, e.g., , , , with which potential gene targets have been identified, e.g., . Coincident with these developments is the continued focus to improve a number of reverse genetic tools to define gene function , , , . Since the first reports in 2003 , , transient RNA interference (RNAi) with either long or short-interfering (si) double-stranded (ds)RNA has proven a straightforward and important tool to study loss of or decreased function for selected genes of interest, including proteases , , , ,kinases , TGF-beta receptor , aquaporin , glucose transporters , tetraspanins  and redox-associated enzymes , . In some of these studies obvious phenotypes that affected parasite vitality and/or survival were obtained. This evidence for RNAi in schistosomes is underpinned by in silico and experimental data to suggest that the necessary RNAi molecular machinery is present , , , . Accordingly, larger scale RNAi screening campaigns of schistosomes can be now envisaged.
To date, RNAi studies with schistosomes relevant to infection in humans have focused on single (or up to 3; , ) genes of interest using different developmental stages and experimental conditions. Thus, the studies only partially contribute to standardizing a set of conditions that are applicable to larger scale investigations of gene function. Furthermore, the degree to which complicating factors such as individual gene susceptibility to RNAi and ‘off-targeting’ (whereby dsRNA can direct the RNAi machinery to target transcripts other than that intended) occur is unknown. The latter phenomenon can confound interpretations of loss-of-function , , , , , , especially when phenotypes are generated . Both issues were highlighted in a recent RNAi phenotypic screen of 32 genes in the mother sporocyst stage of Schistosoma mansoni that parasitizes the snail vector , .
As a pre-requisite to larger RNAi-based screens of schistosome stages infecting humans, we investigated the issues of target selectivity and sensitivity of RNAi for 11 gene transcripts expressed in different tissues of S. mansoni, including genes predicted in silico as being essential to parasite survival . We focused on mechanically transformed schistosomula, which, compared to adult schistosomes, are easier to procure in their hundreds of thousands from snails and adaptable to automated higher throughput screening technologies . Schistosomula also offer the opportunity to identify genes of potential value as chemo- or immuno-therapeutic targets prior to the onset of disease pathology and morbidity associated with egg-laying adults. At the same time, we examined a number of operational parameters (optimal medium for parasite maintenance, transfection strategy and time-, and dose-dependencies of RNAi) for which refinement would better facilitate larger-scale loss-of-function screens.
Materials and Methods
Maintenance of and experiments with vertebrate animals were carried out in accordance with protocols approved by the Institutional Animal Care and Use Committee (IACUC) at UCSF.
S. mansoni life-cycle and preparation of schistosomula
A Puerto Rican isolate of S. mansoni is maintained in the laboratory using the intermediate snail host, Biomphalaria glabrata and the Golden Syrian hamster, Mesocricetus auratus, (infected at 5–6 weeks old; Simonsen) as the definitive host . Methods for the preparation of newly transformed schistosomula (hereafter termed schistosomula) are described previously . Essentially, the procedure involves cleaning cercariae from snail water and mechanically shearing cercarial tails from heads  (the nascent schistosomula) in ice-chilled Basch Incomplete Medium 169  (custom synthesized at the UCSF Cell Culture Facility; containing 100 U/ml penicillin and 100 µg/ml streptomycin, but without FBS). Schistosomula are collected and washed three times in the same medium and kept on ice for a maximum of 1 h prior to dsRNA treatment.
Media evaluation test
To compare the effects of different defined culture media on parasite vitality, 200–300 schistosomula were incubated in 96-well flat-bottom plates (Corning) containing 200 µL medium, 5% FBS and 100 U/ml penicillin and 100 µg/ml streptomycin for up to 7 days at 5% CO2 and 37°C. Media tested were: Basch Medium 169; DMEM (high glucose; UCSF cell culture facility code CCFAA005); F-12 (CCFAB002); Liebovitz's L-15 Medium without NaHCO3 (CCFDY003); M199 with Earle's Salts (CCFAX001); RPMI 1640 (containing 2mM glutamine (CCFAE001) and supplemented with 10 mM HEPES), and Schneider's Drosophila Medium (INVZR512). Phenotypic alterations in translucency, shape and motility  were assessed every second day. Still and time-lapse image recordings were made on the seventh day.
We selected 11 genes encoding diverse proteins and putatively expressed in different tissues (gut, tegument and otherwise) in order to assess any variability in the ability to suppress gene transcription. For gut genes, we chose 3 well-characterized S. mansoni proteases; cathepsin B1 (using a dsRNA targeting both isoforms - CB1.1 (AJ506157) and CB1.2, (AJ506158), cathepsin C (CC, Z32531) and cathepsin D (CD, U60995) , . For the tegument, 3 genes were chosen; CB2 (AJ312106), annexin (XP_002578945.1) and Sm29 (XP_002578407.1). The respective proteins have been bio/immuno-chemically localized to the parasite surface , ,  although CB2 is also localized in the parenchyma . Annexin is also a protein common to most eukaryote plasma membranes  and may, therefore, be more generally distributed in the parasite. The remaining 5 targets are considered to be more generally distributed and have been predicted to be essential in S. mansoni in a recent comparative genomics analysis : glycogen synthase kinase-3 (GSK3; XP_002572305.1), methionine aminopeptidase (MetAP; XP_002572573.1), protein phosphatase-2a (PP-2a; XP_002574514.1), neuroendocrine convertase (NEC; XP_002569775.1) and n-myristoyl transferase (nMT; XP_002571399.1). Table S1 describes the primers used for dsRNA synthesis (the T7 DNA polymerase binding motif is shown in bold characters) and the quantitative real-time (qRT)-PCR primers used to measure RNAi. For dsRNA design, the nucleotide sequence encoding the open reading frame of each gene target was examined using the nucleotide BLAST service at NCBI (constrained to the Schistosoma taxid ID of 6181). Sequence areas displaying homology (especially for identical stretches of 10 nucleotides or more) with other Schistosoma genes were avoided in order to decrease the risk of off-targeting.
Synthesis of dsRNA and Cy5-labeled dsRNA
PCR products of approximately 500 bp in size were amplified from cDNA prepared from 6 day-old in vitro cultivated S. mansoni schistosomula cDNA using gene-specific primers for the targeted genes (Table S1). The expected size of the amplicon was measured by electrophoresis in 1% agarose gels and identity confirmed by DNA sequencing. Amplicons were then used as templates for two more PCR reactions using either a sense or antisense primer containing a 5′-positioned T7 polymerase promoter sequence. After gel purification, each of the two PCR products was separately reverse transcribed in vitro using the T7 RiboMAX express RNAi system (Promega) and the resulting single-stranded RNA pooled to generate dsRNA that was then treated with RNase A and DNase. The manufacturer's instructions were applied apart from scaling the reverse transcription reaction to 100 µl and allowing it to proceed overnight at 37°C with a final hour at 42°C. The dsRNA was precipitated using 1 volume of 10% 3 M sodium acetate (pH 5.2) in 100% isopropanol, and pelleted at 12 000 g for 15 min at 4°C. The pellet was washed in 70% ethanol, air-dried and resuspended in water. The concentration of dsRNA was measured in an ND-1000 Spectrophotometer (Nanodrop Technologies) and adjusted to 3.0 mg/ml. The size and integrity of the resulting dsRNA was confirmed by electrophoresis in 1% agarose gels and stored at −80°C until use.
For synthesis of Cy5-labeled dsRNA to CC, CB1 and the Discosoma sp. fluorescent protein, mCherry (; GenBank AY678264), an aliquot of dsRNA (40 µg) was labeled using the Label IT Nucleic Acid Labeling Reagent (Mirus) according to the manufacturer's instructions. Labeling efficiency was monitored using an ND-1000 Spectrophotometer.
Treatment of schistosomula with dsRNA
Schistosomula were divided into groups of approximately 400 and incubated in 24-well plates containing 1 ml Complete Medium 169 (plus 5% FBS) and dsRNA (30 µg unless otherwise stated) targeting either schistosome transcripts or mCherry that was employed as a schistosome-unspecific control. Incubations were continued for 6 days at 37°C and 5% CO2. To isolate for the effect of electroporation on RNAi efficiency, the following additional incubations were set up. Groups of 400 schistosomula were transferred to 4 mm electroporation cuvets (Bio-Rad Laboratories) in Basch Incomplete Medium 169. DsRNA (30 µg) was then added (usually as a 10 µL aliquot) in Incomplete Medium 169 to give a final volume of 100 µl. Electroporation was carried at 125 V for 20 ms  using a Bio-Rad GenePulser Xcell. Worms were then suspended in 1 ml Complete Medium 169 and incubations continued for 6 days. A second group of worms was electroporated as above, but following electroporation, worms were washed 10 times in Incomplete Medium 169 to remove as much dsRNA as possible and then incubated at 37°C. A third group was electroporated in the absence of dsRNA, and immediately thereafter dsRNA was added for incubation at 37°C.
RNA isolation from schistosomula after dsRNA treatment and conversion to cDNA
After 6 days of cultivation, schistosomula were collected, washed 3 times in 1.5 ml PBS and resuspended in 50 µl of Trizol reagent (Invitrogen). Samples were flash frozen in liquid nitrogen and stored at −80°C. During thawing, samples were homogenized with Kontes disposable pellet pestles and microtubes (VWR). Sample volumes were adjusted to 500 µl with Trizol reagent and the temperature adjusted to 65°C for 5 min to improve tissue lysis. To separate nucleoprotein complexes, samples were further incubated at room temperature for 15–20 minutes. Steps to continue the isolation of total RNA with Trizol were performed up until phase separation in chloroform. At this point, the aqueous phase was transferred to a new 1.5 ml microfuge tube containing an equal volume of 75% ethanol. RNA was further purified using the RNeasy Mini Kit (Qiagen) according to the supplied instructions. The concentration of RNA was determined using an ND-1000 Spectrophotometer. Single-stranded cDNA was synthesized from total RNA using SuperScript III reverse transcriptase (Invitrogen) and an oligo d(T)18 reverse primer according to the manufacturer's protocol. The resulting cDNA was purified using a DNA Clean & Concentrator-5 (Zymo Research Corporation) and stored in water at −80°C.
Primer design and optimization, and qRT-PCR analyses
Primers were designed using the Primer 3 software (http://frodo.wi.mit.edu/, ) employing a target product size of 150–200 bp and a primer melting temperature of 55°C. Primer design excluded outputs containing stretches of four or more of identical nucleotides that might interfere with binding specificity. Primers were designed to bind outside of the region targeted by the dsRNA to avoid the possibility of amplifying carryover PCR product used as a template in dsRNA synthesis reaction. The specificity of each primer sequence was confirmed by BLAST analysis. Primers were purchased from Integrated DNA Technologies at the 25 nM scale and without additional purification procedures. The size of PCR amplicon generated by each primer pair was visually inspected by 1% agarose gel electrophoresis to ensure that the intended sized product was in fact amplified. The amplification efficiency of each qRT-PCR primer set was evaluated in triplicate over at least three and five dilutions of primers and cDNA template, respectively. The cycle threshold (CT) values arising were plotted as a function of the dilution of cDNA . Optimal efficiencies generated curves with slopes of between −3.5 and −3.2 (100% efficiency is represented by a gradient of −3.32). R2 coefficients of ≥0.98 indicated high reproducibility for the triplicate reactions. Primer sets were also checked for their dissociation (melting) curves using the appropriate setting of the MxPro QPCR software package (see below). Only those primers displaying optimal efficiencies and generating single dissociation peaks (the latter demonstrative of a specific reaction without, for example, primer dimerization)  were considered further. Our experience has been that, for each transcript target, two to four primer sets needed to be tested in order to identify an optimal pair.
Quantitative RT-PCR was carried out in an MX 3005P Real-Time PCR cycler (Stratagene, Agilent Technologies, Inc.) using the LightCycler 480 SYBR Green I Master mix (Roche Diagnostics). S. mansoni cytochrome C oxidase I (GenBank AF216698, ) served as the sample normalizing gene transcript . This transcript is highly and constitutively expressed in a number of S. mansoni life-cycle stages . Controls for genomic DNA contamination (minus reverse transcriptase) and reagent purity (water control) were included for each sample. Reactions were carried out in a final volume of 25 µl in 96 well plates (Stratagene). The amplification profile consisted of an initial hot start (95°C for 10 min), followed by 40 cycles comprising 95°C for 30 s, 55°C for 60 s and 72°C for 60 s, and ended with a single cycle of 95°C for 60 s, 55°C for 30 s and 95°C for 30 s. PCR reactions were performed in duplicate for each cDNA sample. Also, at least one additional biological replicate, i.e., with cDNA arising from a separate dsRNA application, was performed. To ensure precision, the entire qPCR experiment was repeated when CT values of duplicate reactions differed by 0.5 or more (indicative of pipeting error). Using the MxPro QPCR Software version 4.01 (Stratagene), the 2−ΔΔCT method  was applied to measure transcript levels following dsRNA treatment. Transcript levels were expressed as a percentage of those following exposure to schistosome-unspecific mCherry dsRNA.
Irreversible active site labeling of SmCC
To verify RNAi of SmCC at the protein level, the fluorescent and selective cathepsin C affinity probe bodipy-labeled FY01  was incubated with protein extract from schistosomula exposed to SmCC-dsRNA. Extracts from worms incubated with mCherry-dsRNA served as a control. Soluble protein extracts were prepared by sonication in 50 mM citrate, 100 mM phosphate, pH 5.0 over an ice bath. After centrifugation at 8 000 g and 4°C for 5 min, supernatants were collected. An aliquot (2 µg protein) was incubated for 1 h at 37°C with 1 µM bodipy-labeled FY01 in 50 mM sodium acetate pH 5.5 containing 2.5 mM DTT, 1 mM EDTA and 1 µM each of the respective aspartic and cathepsin B (cysteine protease) inhibitors, pepstatin A and CA-074 (N-[L-3-trans-propylcarbamoyloxirane- 2-carbonyl]-Ile-Pro-OH ; Bachem). The labeling reaction was stopped by heating to 70°C for 15 min in reducing Laemmli sample buffer. The reaction mixture was resolved by SDS-PAGE through 15% polyacrylamide gels and visualized in a Typhoon 8600 Imager (GE Healthcare) using excitation at 532 nm (green laser) and a 580 nm (BP30 nm) emission filter.
SmCC activity was also detected by hydrolysis of the cathepsin C fluorogenic substrate, Gly-Arg-AMC, as described previously , in the presence and absence of 10 µM of the following protease inhibitors (Bachem): the general Clan CA cysteine protease inhibitor, K11777 (N-methyl-piperazine-Phe-homoPhe-vinyl sulfone phenyl ); the cathepsin B-selective inhibitor, CA-074; and the cathepsin L-preferential inhibitor, Z-Phe-Phe-DMK (benzyloxy carbonyl-Phe-Phe-diazomethane ). Assays were performed in triplicate.
Preparation of recombinant mCherry fluorescent protein
The mushroom anemone (Discosoma sp.) red fluorescent protein, mCherry , was recombinantly expressed in TOP10 chemically competent E. coli (Invitrogen). Bacteria that had been transformed with the mCherry open reading frame as part of a pUC18 plasmid backbone were grown overnight at 37°C in 500 ml LB medium containing 100 µg/ml of ampicillin and 20 mM glucose. After centrifugation at 3 000 g for 10 min, bacteria were incubated for 4 h in fresh LB medium containing 1 mM IPTG (isopropyl-β-D-thio-galactoside) and harvested by centrifugation for 10 min at 4 000 g. The pellet was re-suspended in 50 ml 100 mM phosphate buffer, pH 8.0, and sonicated using a Branson Sonifier 250 adjusted to a 60% duty cycle and an output control of 6. The material was centrifuged at 10 000 g for 15 min and the supernatant, containing recombinant mCherry, was collected. Heat denaturation in a water bath at 80°C for 40 min followed by centrifugation at 10 000 g for 15 min was employed to separate bacterial proteins from the heat-stable mCherry protein in the supernatant. The supernatant was decanted and concentrated over an Amicon Ultra-50 10K (Millipore) filter to a final volume of 5 ml. The sample was size fractionated over a Superdex 200 column (GE- Healthcare) pre-equilibrated with 100 mM phosphate buffer, pH 8.0. Eluted fractions containing visible red mCherry protein (4 mg/ml) were pooled and stored at −20°C.
Uptake of exogenous material by schistosomula in vitro
Schistosomula were co-incubated in 1 ml Complete Medium 169 with either 30 µg Cy5-labeled SmCB1- or mCherry- dsRNA, 200 µg recombinant mCherry protein or 2 µL washed and packed human erythrocytes. Uptake was monitored as a function of time, usually every half hour out to 4 h, at 20 h and thereafter, every day out to 4 days. For Cy5-labeled dsRNA, worms were washed 4 times in Incomplete Medium 169 before imaging with a laser scanning microscope (LSM 510 META; Carl Zeiss MicroImaging, Inc.). For mCherry protein, fluorescence was visualized without washing using an Axiovert 40CFL inverted microscope. Bright field images of parasites in the presence of erythrocytes were acquired using an Axiovert 40C inverted microscope.
Of seven defined culture media tested, Basch Medium 169 optimally maintains schistosomula
Prior to RNAi experiments with newly transformed schistosomula, we wished to evaluate the effects of different defined culture media (containing 100 U/ml penicillin and 100 µg/ml streptomycin and 5% FBS) on parasite vitality and viability. These effects were visually determined as phenotypic alterations in parasite motility, shape and translucence . Time-lapse image recordings, taken at day 7 of the incubation, are provided as Videos S1, S2, S3, S4, S5, S6, S7 and provide the best visual medium to compare and contrast the worm responses to the incubation conditions. Still images from these experiments are also presented in Figure 1. By the seventh day of incubation, relative to Basch medium 169, DMEM (high glucose), F-12, Liebowitz and M199 media had generated varying degrees of deformed, rounded and/or darkened parasites, often accompanied by slowed and less dynamic motility. In RPMI and Schneider's media, additional and more severe effects (degenerating parasites and widespread death) were noted, which appeared as early as day 2 of the incubation and progressed to approximately >80% parasite mortality by day 7. For RPMI, this was also case when the medium was exchanged every 2 days. In contrast, in Basch medium 169, parasites were more uniform in translucency, shape and motility with less than 10% mortality at day 7. Based on these results, therefore, we employed Basch medium 169 for all further experiments.
The issue of carry-over contaminating DNA in accurately measuring RNAi
In spite of the mandatory DNase treatment used to remove PCR-derived DNA from dsRNA preparations, the possibility existed that residual DNA would nevertheless serve as template for qPCR should the qPCR primers be designed to amplify within the sequence used to synthesize dsRNA , . This would result in an apparent lack of, or less efficient, transcript knockdown. That this is a legitimate concern (and even with best practice to purify dsRNA according to the appropriate protocol) is shown in Figure S1 using ‘purified’ dsRNA to the tegument-associated SmCB2 transcript as an example. A concentration-dependent amplification resulted (Figure S1A) when qPCR primers (Primer set B) were positioned within the sequence employed as template for reverse transcription (Figure S1B). In contrast, primer set A, that targeted an area 5′ of the dsRNA template sequence, produced no such amplification.
Direct toxicity of dsRNA to schistosomula, and dose- and time-dependency of RNAi
Direct toxicity to schistosomula by dsRNA was evident as greater proportions of parasites that displayed abnormal phenotypes  such as rounding, darkening, slowed motility and outright death, or a combination thereof (Figure 2). Using dsRNA to mCherry (or CB1, not shown) and at the highest concentration tested of 210 µg/ml, more than 35% of the parasites appeared abnormal by days 3 and 6 of the incubation (Figure 2A). At a concentration of 150 µg/ml, a marked increase in the percentage of distressed parasites was measured between days 3 (12%) and 6 (35%; Figure 2A and 2B, lower panel). Concentrations below this (30 and 90 µg/ml) did not increase the percentage of abnormal parasites above the 12% background for the duration of the experiment. Thus, we interpret the data to suggest that the upper concentration limit for dsRNA, without incurring obvious parasite distress, is approximately 90 µg/ml. Operationally, we chose 30 µg/ml dsRNA to provide an increased margin of safety and based on the dsRNA dose-dependency tests for CB1 (Figure 3A), MetAP, NEC and PP2a (not shown) indicating that 30 µg/ml was sufficient for maximal RNAi. We chose a period of 6 days for co-incubating dsRNA and parasites as it was sufficient to register maximal transcriptional suppression of CB1, MetAP and PP2a (Figure 3B, C and D, respectively).
RNAi, including more than one transcript target, is selective, however, its efficiency is gene-dependent
Transcripts for the gut-associated proteins CB1, CC and CD are sensitive to RNAi and are robustly suppressed by >75% (Figure 4A, B and C, respectively). Importantly, the effect is selective for the intended mRNA within the pool of gut transcripts examined. Attachment of the fluorescent Cy5 label to CB1-(Figure 4A) or CC-(not shown) specific dsRNA does not interfere with suppression, or the degree of suppression, of the cognate mRNA. With non-labeled dsRNA targeting CB1, CC and CD, the RNAi effect was long-term, remaining constant out to 3 weeks of incubation without exchanging either culture medium or dsRNA (not shown). For CB1, long-term suppression (>30 days of incubation) of transcript has been previously noted for schistosomula exposed at 3 h  and 7 days post-transformation .
As exemplified by CB1 and CD (Figure 5A and B, respectively), RNAi of more than one transcript at the same time is possible in schistosomula (30 µg/ml dsRNA per transcript target), in this case with >75% suppression. As found for RNAi of single transcripts, the effect is again selective within the pool of transcripts examined. For these experiments, the transcript for cystatin (Cys), a cysteine protease inhibitor (AY334553.1) , was included as a non-targeted transcript control.
Confirmation of RNAi at the protein level was sought for CC (Figure 6). A 6-day co-incubation of schistosomula and dsRNA targeting CC realized a knockdown of the translation product, measured either as an 80% decrease in the peptidolytic cleavage of the cathepsin C-selective substrate, GR-AMC (Figure 6A) or as a loss of the proteolytic signal (at approximately 24 kDa) using the specific active site-directed probe, bodipy-labeled FY01 (Figure 6B). Activity in the presence of GR-AMC was not inhibited by the general cysteine protease inhibitor, K11777, or by CA-074 and Z-Phe-Phe-DMK that target cathepsins B and L, respectively. This indicates that only cathepsin C activity was being measured. For CB1, a similar absence of protein was recorded after dsRNA application using immunoblotting with monospecific antibodies (not shown).
Robust and selective single and double knockdowns (>75%) were also possible for the tegument transcript targets CB2, annexin and Sm29 using 30 µg/ml dsRNA per transcript (Figure 7A, B and C, respectively). This was the case within the pool of tegument transcripts examined and as compared to the gut-associated CB1 employed as a ‘tissue control’.
For the 5 remaining genes that are considered to be more generally distributed (MetAP, nMT, PP2a, GSK3, and NEC), selective single and double (for MetAP, nMT and PP2a) knockdowns were generated with 30 µg/ml dsRNA per transcript (Figure 8). However, compared to the gut and tegument targets, the efficiency of knockdown was more variable. Thus, a decrease of approximately 75% was recorded for MetAP (Figure 8A); whereas for nMT and PP2a, decreases of between 40 and 60% were obtained (Figure 8B and C). GSK3 and NEC transcripts were more resistant to RNAi with maximum decreases of approximately 40% (Figure 8D and E). RNAi of each target did not affect levels of CB1 transcript used as a ‘tissue control’. Likewise, RNAi of CB1 did not affect transcript levels of the other gene targets (Figure 8F). Doubling or tripling the concentration to 90 µg/ml dsRNA did not improve RNAi (not shown), nor was it the case that transcript suppression was greater earlier in the 6-day incubation period (as shown when processing samples for transcript levels of MetAP and PP2a at days 2 and 4 of the incubation; Figure 3C). Thus, in the present experimental system, the RNAi recorded seems to be the maximal achievable.
No striking or obvious phenotypes were observed upon RNAi of any of the 11 gene transcripts over the 6-day incubation period. For the 3 gut-associated and 5 in silico-predicted essential genes, this was also the case after 3 week co-incubations and whether or not the culture medium and dsRNA were exchanged weekly (not shown). For CB1 and CD, the same was also true in the presence of washed erythrocytes and was independent of whether single or double RNAi was employed (not shown).
The contribution by electroporation to RNAi is minor and may depend on the tissue localization of the target
Electroporation has been employed as part of a protocol to deliver nucleic acids to both adult schistosomes and schistosomula e.g., , , , . Our experience with electroporation has entailed some risk of contamination of parasite culture consequent on the extra handling required to perform this technique. These issues are of logistical importance when streamlining larger scale RNAi experiments. We, therefore, devised a series of experimental conditions for selected gene targets to determine the contribution to RNAi by electroporation as compared to simple co-incubation.
Co-incubation of dsRNA with schistosomula or electroporation in the presence or absence of dsRNA followed by co-incubation of dsRNA with parasites led to robust transcript suppression (>75%) for each the tissue-representative targets examined, namely CB1 (gut), CB2 (tegument) and MetAP (otherwise; Figure 9). In contrast, when worms that had been electroporated in the presence of dsRNA were then immediately washed 10 times prior to incubation in the absence of dsRNA, the RNAi effect was more limited. Thus, for the gut proteases CB1 (Figure 9A), CC and CD (not shown), transcript levels were decreased by between 0 and 15% relative to mCherry. Similarly, only an approximate 20% decrease in message was recorded for MetAP when the contribution of electroporation to RNAi was isolated (Figure 9C). However, for the tegument targets, CB2 (Figure 9B), annexin and Sm29 (not shown), electroporation alone generated a more apparent RNAi of approximately 50%. This suggests that tegument targets may be more amenable to electroporation, perhaps facilitated by their greater proximity to the transducing electrical field generated by electroporation. Yet, overall, the contribution of electroporation, when isolated from the other experimental parameters, to the RNAi effect is relatively minor, and consequently could be omitted as an operational parameter when streamlining larger-scale screens with dsRNA. We note, however, that what may apply for optimal delivery of long dsRNA may not be the case for siRNA.
The gut as a mediator of RNAi in newly transformed schistosomula
Given the above data suggesting that simple co-incubation of newly transformed parasites and dsRNA is sufficient to mediate RNAi, we then asked the question whether the gut serves as a route of entry for dsRNA into the schistosome. The question is relevant in view of previous discussions to the effect that the parasite mouth is not open until about day 7 of the incubation (i.e., indicative of a non-functioning gut) and, therefore, cannot facilitate RNAi in younger schistosomula . Using Cy5-labeled dsRNA to CB1, CC or mCherry, we demonstrate that the gut of schistosomula takes up and concentrates exogenous material within minutes of their mechanical preparation (Figure 10; results with CB1 dsRNA shown). Accumulation of the dye was evident along the gut and in the two terminal cecal chambers by 90 min post-transformation (Figure 10A). At the same time-point, a fluorescent signal from accumulated mCherry protein could be detected (Figure 10B). For both probes, the signal remained visible during the incubation period of 6 days illuminating the gut ceca as they elongated posteriorly over time. Consistent with this rapid onset of gut activity, we could already measure RNAi of CB1 at between 2 and 4 h post-incubation with dsRNA, an effect that increased as a function of time (Figure 3B). Finally, we could detect hemoglobin-derived pigment in the gut of 2 day-old schistosomula co-incubated with erythrocytes (Figure 10C). Taken together, the results suggest that the schistosomulum gut is functionally active within minutes of their preparation from cercariae and seems a likely route through which RNAi is mediated.
Large-scale RNA-dependent gene silencing is an informative reverse genetic platform to interrogate decreased or loss of gene function at the protein, pathway, cell and organism levels. RNAi can also aid the identification of drug targets for the treatment of a variety of disease states , , , including those caused by pathogens , . For schistosomes, a number of key components (comprehensive genome and transcriptome data, interrogable databases, and both in silico and experimental evidence of the RNAi phenomenon) are now in place to facilitate the implementation of larger scale RNAi-based screening campaigns.
In advance, we investigated the sensitivity and selectivity of RNAi for a set of 11 genes, which contained representatives from different tissue types – gut, tegument and otherwise. We employed long (approximately 500 bp) dsRNA rather than siRNA as the former can be produced at scales sufficient for performing multiple experiments and based on the assumption that the variety of siRNAs generated from the parent dsRNA would provide for as much silencing potential as possible. We focused on mechanically transformed schistosomula that are both relevant to parasitism in humans and, compared to adult parasites, available in greater numbers for automated high throughput screening . In addition, issues of operational efficiency and simplicity were also considered, including, choice of culture medium, transfection strategy, dsRNA time- and dose-dependency and dosing limits. It was during these studies that the gut was identified as a likely route for entry of dsRNA into the newly transformed parasite.
The question of which culture medium to use for optimal maintenance of schistosomula has not been directly evaluated since the pioneering culture work of Cheever and Weller, and Clegg in the 1950s and 60s , , and Basch in the early 1980's . The issue is also relevant to chemical drug discovery programs that involve co-incubation of schistosomula with compounds and the recording of phenotypes . Of the seven media tested here (Basch Medium 169, DMEM, F-12, Liebowitz's, M199, RPMI 1640 and Schneider's), all except Basch Medium 169 induced abnormal phenotypes amounting to darkened and/or distorted parasites often accompanied by slowed and less dynamic motility. In the case of Schneider's and RPMI 1640 media, widespread death occurred. Of note was the poor survival of schistosomula in RPMI 1640, a medium that has been previously employed for a number of RNAi studies with schistosomula , , , . Our concern was that the high background mortality observed here would make determinations of an RNAi effect, either at the mRNA level or, certainly, at the phenotypic level, difficult. Yet, despite the poor survival in RPMI, RNAi of CB1 transcript could be decreased to levels similar to those achieved in Basch Medium 169 (data not shown) - a finding consistent with the effect observed previously. Nevertheless, based on the present media tests, we employ Basch Medium 169 as the medium of choice for maintenance of S. mansoni schistosomula as mortality remained at approximately 10% out to 4 weeks of incubation. Indeed, consistent with the earlier report , we have been able to maintain parasites long enough (about 8 months) in Basch Complete Medium 169 to the point of pairing of males and females (unpublished data).
Key concerns in any large scale screening campaign are the occurrence and extent of off-targeting. The possibility of such phenomena operating in schistosomes has been raised in a recent report involving RNAi of 32 genes expressed in S. mansoni mother sporocysts , . For the smaller set of 11 genes studied here in schistosomula, we did not observe off-targeting which is encouraging for larger scale studies. RNAi, including suppression of more than one transcript, was selective to the intended target(s) among and between the different pools of gut, tegument and generally distributed transcripts investigated. The finding is also important as it expands upon previous data for smaller numbers (1 to 3) of genes of interest ,  that demonstrated RNAi specificity relative to schistosome-unspecific dsRNA preparations used as controls. The apparent lack of off-targeting in newly transformed schistosomula is consistent with data from similarly designed experiments for a pool of gut-associated proteases in 3-week old S. mansoni . That the contemporaneous and selective suppression of more than one gene is possible in schistosomula could prove useful in interrogating functional redundancy within protein systems. An example of such is the network of gut proteases that digest host proteins , . Multiple RNAi of component proteases may help reveal the extent of redundancy and highlight cooperative relationships in how host proteins are processed.
For both the gut and tegument genes studied, knockdown efficiency, including after double-targeting, was at least 75%. For the gut proteases, CC and CB1, transcript knockdown was mirrored by a substantial decrease or loss of translation product as determined biochemically with a specific substrate and active site-directed probe for CC, and a monospecific anti-serum to CB1. The robust suppression of CB1 and CD recorded here is consistent with previous studies using schistosomula , ,  and 3 week-old sub-adult worms . Though our observations did not include measurements of worm size to statistically discriminate dsRNA-induced phenotypic alterations , , we noted no striking abnormalities in parasites co-incubated for 3 weeks with dsRNA to CB1 and/or CD in the presence or absence of erythrocytes. This included a previous observation of red-colored, rather than the normally black, gut contents in a population of schistosomula exposed to CD-dsRNA in the presence of erythrocytes– a finding that prompted the suggestion that diminished CD activity was responsible for the phenotype . At this time, we cannot explain the difference in phenotypic outcomes, however, we observe that the color of gut contents of individual schistosomula normally varies from black to brown to red when cultured in the presence of erythrocytes.
The robust RNAi of gut and tegument targets contrasts with the more variable efficiencies recorded for the 5 gene targets considered to be more generally distributed in the parasite and that had been predicted in silico to be essential . Thus, the efficiency of RNAi ranged from approximately 75% (MetAP) to approximately 40% for GSK3 and NEC. We speculate that the localization of gene expression is in part responsible for this variation whereby genes expressed in the gut and tegument are more likely to be efficiently suppressed given their proximity to the external environment containing dsRNA.
Such gene-to-gene variability in sensitivity to RNAi is likely inherent when, under a standardized set of conditions such as those under investigation here, not all gene transcripts will respond to the same degree. Variable gene responses to RNAi have also been recently described for S. mansoni mother sporocysts ,  and schistosomula  exposed to dsRNA. Transcripts not decreased below certain critical thresholds will contribute to the lack of obvious phenotypes arising. This could also explain why targeting the 5 genes that had been predicted in silico as being essential to S. mansoni  produced no gross phenotypic alterations, even at near toxic dsRNA concentrations or over an extended 3-week co-incubation period. Of course, this is aside from the more direct possibility that the genes are not, after all, essential to survival of the parasite or the particular developmental stage in question. In addition, and as discussed previously , even for genes that are sensitive to RNAi, issues such as developmental plasticity of the schistosome and functional redundancy in protein function, pathways or networks will limit the number of readily observable phenotypes. Yet, given these considerations, literature reports of striking phenotypes post-RNAi in schistosomula (e.g., , ) would encourage continued loss-of-function investigations, especially in view of the imperative to identify new drug targets.
The finding that co-incubation of newly transformed parasites and dsRNA, without the need for electroporation (i.e., trans-tegumental delivery of dsRNA), is sufficient to induce robust RNAi focused our attention on the parasite gut in dsRNA uptake. By 90 min post-production from cercariae we could observe a concentration of fluorescent macromolecules above background in the gut that was indicative of rapid and active ingestion of exogenous material by the parasite. This gut activity during transformation from the cercarial state is consistent with electron microscopy studies demonstrating rearrangements of the esophageal environment  and earlier observations that transforming parasites engulf culture fluid . In parallel with the ingestion of fluorescent materials, we could measure decreased CB1 transcript 2 to 4 hours after co-incubation of worms with specific dsRNA suggesting that dsRNA, upon ingestion, is both rapidly absorbed and processed to initiate mRNA suppression. It is possible that the tegument or other organ systems such as the acetabular glands  or nephridiopore  also act as entry points for dsRNA in nascent schistosomula, however, the present results suggest that the gut should also be considered as a key contributor. Finally, the observation of blood products in 2 day-old schistosomula reinforces the evidence that the gut is quickly put to work by the parasite in the acquisition of nutrients.
The data reported herein that newly transformed schistosomula are amenable to RNAi is promising in view of previous reports observing less efficient or inconsistent suppression (of CB1) in parasites <7days old , . The underlying reasons for these apparent differences in RNAi kinetics are unclear and could be reviewed as part of a broader discussion on assay development and refinement. From the perspective of screen design, however, it is logistically much simpler to move directly from ‘snails to screens’ employing newly transformed schistosomula without factoring for both the extra time and handling required for older parasites, particularly as they seem to be more susceptible to handling damage than newly transformed schistosomula (unpublished data).
The influence of qPCR primer positioning on the amplification of residual PCR product carried over from dsRNA synthesis. There is no amplification of residual PCR product (Panel A) when primer pairs (Primer set A) are positioned upstream from that part of the open reading frame of SmCB2 used to synthesize dsRNA (Panel B). In contrast, Primer set B (positioned within the sequence used to generate dsRNA) amplifies residual PCR product in a concentration-dependent manner that would negatively impact the apparent efficiency of RNAi as measured by qRT-PCR. Each sample was tested in duplicate and representative data from two experiments are shown.
(0.35 MB TIF)
List of primer sequences employed for dsRNA synthesis (the T7 DNA polymerase binding motif is shown in bold characters) and quantitative real-time (qRT)-PCR.
(0.03 MB XLS)
Phenotypic responses of S. mansoni schistosomula upon incubation in Basch Medium 169. Parasites were maintained for 7 days in medium containing 5% FBS, and 100 U/ml penicillin and 100 µg/ml streptomycin. Time-lapse recordings were captured with a Zeiss Axiovert 40 C inverted microscope (10× magnification) connected to a Zeiss AxioCam MRc digital camera that was under the control of AxioVision 40 version 220.127.116.11 software. Note that parasites in this medium appear most uniform in translucency, shape and motion with the least background mortality.
(3.66 MB MOV)
Phenotypic responses of S. mansoni schistosomula upon incubation in DMEM (high glucose) medium. Parasites were maintained for 7 days in medium containing 5% FBS, and 100 U/ml penicillin and 100 µg/ml streptomycin. Time-lapse recordings were captured with a Zeiss Axiovert 40 C inverted microscope (10× magnification) connected to a Zeiss AxioCam MRc digital camera that was under the control of AxioVision 40 version 18.104.22.168 software.
(3.53 MB MOV)
Phenotypic responses of S. mansoni schistosomula upon incubation in F-12 medium. Parasites were maintained for 7 days in medium containing 5% FBS, and 100 U/ml penicillin and 100 µg/ml streptomycin. Time-lapse recordings were captured with a Zeiss Axiovert 40 C inverted microscope (10× magnification) connected to a Zeiss AxioCam MRc digital camera that was under the control of AxioVision 40 version 22.214.171.124 software.
(3.47 MB MOV)
Phenotypic responses of S. mansoni schistosomula upon incubation in Liebowitz's medium. Parasites were maintained for 7 days in medium containing 5% FBS, and 100 U/ml penicillin and 100 µg/ml streptomycin. Time-lapse recordings were captured with a Zeiss Axiovert 40 C inverted microscope (10× magnification) connected to a Zeiss AxioCam MRc digital camera that was under the control of AxioVision 40 version 126.96.36.199 software.
(3.44 MB MOV)
Phenotypic responses of S. mansoni schistosomula upon incubation in M199 medium with Earle's Salts. Parasites were maintained for 7 days in medium containing 5% FBS, and 100 U/ml penicillin and 100 µg/ml streptomycin. Time-lapse recordings were captured with a Zeiss Axiovert 40 C inverted microscope (10× magnification) connected to a Zeiss AxioCam MRc digital camera that was under the control of AxioVision 40 version 188.8.131.52 software.
(4.30 MB MOV)
Phenotypic responses of S. mansoni schistosomula upon incubation in RPMI 1640 medium. Parasites were maintained for 7 days in medium containing 10 mM HEPES, 2 mM glutamine, 5% FBS, and 100 U/ml penicillin and 100 µg/ml streptomycin. Time-lapse recordings were captured with a Zeiss Axiovert 40 C inverted microscope (10× magnification) connected to a Zeiss AxioCam MRc digital camera that was under the control of AxioVision 40 version 184.108.40.206 software.
(2.81 MB MOV)
Phenotypic responses of S. mansoni schistosomula upon incubation in Schneider's medium. Parasites were maintained for 7 days in medium containing 5% FBS, and 100 U/ml penicillin and 100 µg/ml streptomycin. Time-lapse recordings were captured with a Zeiss Axiovert 40 C inverted microscope (10× magnification) connected to a Zeiss AxioCam MRc digital camera that was under the control of AxioVision 40 version 220.127.116.11 software.
(2.63 MB MOV)
Conceived and designed the experiments: SŠ JD MH SB DS CRC. Performed the experiments: SŠ JD MH SB DS DSR BS K-CL SDH CRC. Analyzed the data: SŠ JD MH SB DS DSR BS CRC. Wrote the paper: SŠ JD MH JHM CRC.
- 1. Hotez PJ,Fenwick A,Savioli L,Molyneux DH (2009) Rescuing the bottom billion through control of neglected tropical diseases. Lancet 373: 1570–1575.
- 2. Vieira P,Miranda HP,Cerqueira M,Delgado Mde L,Coelho H,et al. (2007) Latent schistosomiasis in Portuguese soldiers. Mil Med 172: 144–146.
- 3. Bell RM,Daly J,Kanengoni E,Jones JJ (1973) The effects of endemic schistosomiasis and of hycanthone on the mental ability of African school children. Trans R Soc Trop Med Hyg 67: 694–701.
- 4. Gryseels B,Polman K,Clerinx J,Kestens L (2006) Human schistosomiasis. Lancet 368: 1106–1118.
- 5. Hotez PJ,Fenwick A,Kjetland EF (2009) Africa's 32 Cents Solution for HIV/AIDS. PLoS Negl Trop Dis 3: e430.
- 6. Jukes MC,Nokes CA,Alcock KJ,Lambo JK,Kihamia C,et al. (2002) Heavy schistosomiasis associated with poor short-term memory and slower reaction times in Tanzanian schoolchildren. Trop Med Int Health 7: 104–117.
- 7. Wu XH,Wang TP,Lu DB,Hu HT,Gao ZB,et al. (2002) Studies of impact on physical fitness and working capacity of patients with advanced Schistosomiasis japonica in Susong County, Anhui Province. Acta Trop 82: 247–252.
- 8. Badawi AF,Mostafa MH,Probert A,O'Connor PJ (1995) Role of schistosomiasis in human bladder cancer: evidence of association, aetiological factors, and basic mechanisms of carcinogenesis. Eur J Cancer Prev 4: 45–59.
- 9. Rollinson D (2009) A wake up call for urinary schistosomiasis: reconciling research effort with public health importance. Parasitology 136: 1593–1610.
- 10. Kjetland EF,Ndhlovu PD,Gomo E,Mduluza T,Midzi N,et al. (2006) Association between genital schistosomiasis and HIV in rural Zimbabwean women. AIDS 20: 593–600.
- 11. Caffrey CR (2007) Chemotherapy of schistosomiasis: present and future. Current Opinion in Chemical Biology 11: 433–439.
- 12. Cioli D,Pica-Mattoccia L (2003) Praziquantel. Parasitol Res 90: Supp 1S3–9.
- 13. Doenhoff MJ,Pica-Mattoccia L (2006) Praziquantel for the treatment of schistosomiasis: its use for control in areas with endemic disease and prospects for drug resistance. Expert Rev Anti Infect Ther 4: 199–210.
- 14. Doenhoff MJ,Hagan P,Cioli D,Southgate V,Pica-Mattoccia L,et al. (2009) Praziquantel: its use in control of schistosomiasis in sub-Saharan Africa and current research needs. Parasitology 136: 1825–1835.
- 15. Botros S,Sayed H,Amer N,El-Ghannam M,Bennett JL,et al. (2005) Current status of sensitivity to praziquantel in a focus of potential drug resistance in Egypt. Int J Parasitol 35: 787–791.
- 16. Melman SD,Steinauer ML,Cunningham C,Kubatko LS,Mwangi IN,et al. (2009) Reduced susceptibility to praziquantel among naturally occurring Kenyan isolates of Schistosoma mansoni. PLoS Negl Trop Dis 3: e504.
- 17. Smits HL (2009) Prospects for the control of neglected tropical diseases by mass drug administration. Expert Review of Anti-infective Therapy 7: 37–56.
- 18. Brady MA,Hooper PJ,Ottesen EA (2006) Projected benefits from integrating NTD programs in sub-Saharan Africa. Trends Parasitol 22: 285–291.
- 19. Hotez PJ,Molyneux DH,Fenwick A,Ottesen E,Ehrlich Sachs S,et al. (2006) Incorporating a rapid-impact package for neglected tropical diseases with programs for HIV/AIDS, tuberculosis, and malaria. PLoS Med 3: e102.
- 20. Lammie PJ,Fenwick A,Utzinger J (2006) A blueprint for success: integration of neglected tropical disease control programmes. Trends Parasitol 22: 313–321.
- 21. Molyneux DH,Hotez PJ,Fenwick A (2005) “Rapid-impact interventions”: how a policy of integrated control for Africa's neglected tropical diseases could benefit the poor. PLoS Med 2: e336.
- 22. Utzinger J,Raso G,Brooker S,De Savigny D,Tanner M,et al. (2009) Schistosomiasis and neglected tropical diseases: towards integrated and sustainable control and a word of caution. Parasitology 136: 1859–1874.
- 23. Abdul-Ghani RA,Loutfy N,Hassan A (2009) Experimentally promising antischistosomal drugs: a review of some drug candidates not reaching the clinical use. Parasitol Res 105: 899–906.
- 24. Abdulla MH,Lim KC,Sajid M,McKerrow JH,Caffrey CR (2007) Schistosomiasis mansoni: novel chemotherapy using a cysteine protease inhibitor. PLoS Med 4: e14.
- 25. Corrêa Soares JB,Menezes D,Vannier-Santos MA,Ferreira-Pereira A,Almeida GT,et al. (2009) Interference with hemozoin formation represents an important mechanism of schistosomicidal action of antimalarial quinoline methanols. PLoS Negl Trop Dis 3: e477.
- 26. Keiser J,Utzinger J (2007) Advances in the discovery and development of trematocidal drugs. Expert Opin Drug Discov 2: Suppl. 1S9–S23.
- 27. McManus DP,Loukas A (2008) Current status of vaccines for schistosomiasis. Clin Microbiol Rev 21: 225–242.
- 28. Fitzpatrick JM,Peak E,Perally S,Chalmers IW,Barrett J,et al. (2009) Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. PLoS Negl Trop Dis 3: e543.
- 29. Gobert GN,Moertel L,Brindley PJ,McManus DP (2009) Developmental gene expression profiles of the human pathogen Schistosoma japonicum. BMC Genomics 10: 128.
- 30. Jolly ER,Chin CS,Miller S,Baghat MM,Lim KC,et al. (2007) Gene expression patterns during adaptation of a parasite helminth to different environmental niches. Genome Biol 8: R65.
- 31. Liu F,Lu J,Hu W,Wang SY,Cui SJ,et al. (2006) New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. PLoS Pathog 2: e29.
- 32. Oliveira G,Franco G,Verjovski-Almeida S (2008) The Brazilian contribution to the study of the Schistosoma mansoni transcriptome. Acta Trop 108: 179–182.
- 33. Verjovski-Almeida S,DeMarco R,Martins EA,Guimarães PE,Ojopi EP,et al. (2003) Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni. Nat Genet 35: 148–157.
- 34. Verjovski-Almeida S,Venancio TM,Oliveira KC,Almeida GT,Demarco R (2007) Use of a 44k oligoarray to explore the transcriptome of Schistosoma mansoni adult worms. Exp Parasitol 117: 236–245.
- 35. Williams DL,Sayed AA,Bernier J,Birkeland SR,Cipriano MJ,et al. (2007) Profiling Schistosoma mansoni development using serial analysis of gene expression (SAGE). Exp Parasitol 117: 246–258.
- 36. Hu W,Yan Q,Shen DK,Liu F,Zhu ZD,et al. (2003) Evolutionary and biomedical implications of a Schistosoma japonicum complementary DNA resource. Nat Genet 35: 139–147.
- 37. Berriman M,Haas BJ,LoVerde PT,Wilson RA,Dillon GP,et al. (2009) The genome of the blood fluke Schistosoma mansoni. Nature 460: 352–358.
- 38. Liu F,Zhou Y,Wang ZQ,Lu G,Zheng H,et al. (2009) The Schistosoma japonicum genome reveals features of host-parasite interplay. Nature 460: 345–351.
- 39. Tsai I,Otto TD,Berriman M (2010) Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps. Genome Biology 11: R41.
- 40. Liu F,Chen P,Cui SJ,Wang ZQ,Han ZG (2008) SjTPdb: integrated transcriptome and proteome database and analysis platform for Schistosoma japonicum. BMC Genomics 9: 304.
- 41. Zerlotini A,Heiges M,Wang H,Moraes RL,Dominitini AJ,et al. (2009) SchistoDB: a Schistosoma mansoni genome resource. Nucleic Acids Res 37: D579–582.
- 42. Agüero F,Al-Lazikani B,Aslett M,Berriman M,Buckner FS,et al. (2008) Genomic-scale prioritization of drug targets: the TDR Targets database. Nat Rev Drug Discov 7: 900–907.
- 43. Beckstette M,Mailänder JT,Marhöfer RJ,Sczyrba A,Ohlebusch E,Giegerich R,Selzer PM (2004) Genlight: interactive high-throughput sequence analysis and comparative genomics. Journal of Integrative Bioinformatics 1:
- 44. Margulies EH,Birney E (2008) Approaches to comparative sequence analysis: towards a functional view of vertebrate genomes. Nat Rev Genet 9: 303–313.
- 45. Ptacek T,Sell SM (2005) A tiered approach to comparative genomics. Brief Funct Genomic Proteomic 4: 178–185.
- 46. Caffrey CR,Rohwer A,Oellien F,Marhöfer RJ,Braschi S,et al. (2009) A comparative chemogenomics strategy to predict potential drug targets in the metazoan pathogen, Schistosoma mansoni. PLoS ONE 4: e4413.
- 47. Brindley PJ,Pearce EJ (2007) Genetic manipulation of schistosomes. Int J Parasitol 37: 465–473.
- 48. Dvořák J,Beckmann S,Lim KC,Engel JC,Grevelding CG,et al. (2010) Biolistic transformation of Schistosoma mansoni: Studies with modified reporter-gene constructs containing regulatory regions of protease genes. Mol Biochem Parasitol 170: 37–40.
- 49. Grevelding CG (2006) Transgenic flatworms. In: Maule AGMNJ, editor. Parasitic flatworms: molecular biology, biochemistry, immunology and physiology. Wallingford: CAB International. pp. 149–173.
- 50. Mann VH,Morales ME,Kines KJ,Brindley PJ (2008) Transgenesis of schistosomes: approaches employing mobile genetic elements. Parasitology 135: 141–153.
- 51. Boyle JP,Wu XJ,Shoemaker CB,Yoshino TP (2003) Using RNA interference to manipulate endogenous gene expression in Schistosoma mansoni sporocysts. Mol Biochem Parasitol 128: 205–215.
- 52. Skelly PJ,Da'dara A,Harn DA (2003) Suppression of cathepsin B expression in Schistosoma mansoni by RNA interference. Int J Parasitol 33: 363–369.
- 53. Correnti JM,Brindley PJ,Pearce EJ (2005) Long-term suppression of cathepsin B levels by RNA interference retards schistosome growth. Mol Biochem Parasitol 143: 209–215.
- 54. Delcroix M,Sajid M,Caffrey CR,Lim KC,Dvořák J,et al. (2006) A multienzyme network functions in intestinal protein digestion by a platyhelminth parasite. J Biol Chem 281: 39316–39329.
- 55. Morales ME,Rinaldi G,Gobert GN,Kines KJ,Tort JF,et al. (2008) RNA interference of Schistosoma mansoni cathepsin D, the apical enzyme of the hemoglobin proteolysis cascade. Mol Biochem Parasitol 157: 160–168.
- 56. Rinaldi G,Morales ME,Alrefaei YN,Cancela M,Castillo E,et al. (2009) RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs. Mol Biochem Parasitol 167: 118–126.
- 57. Swierczewski BE,Davies SJ (2009) A schistosome cAMP-dependent protein kinase catalytic subunit is essential for parasite viability. PLoS Negl Trop Dis 3: e505.
- 58. Osman A,Niles EG,Verjovski-Almeida S,LoVerde PT (2006) Schistosoma mansoni TGF-beta receptor II: role in host ligand-induced regulation of a schistosome target gene. PLoS Pathog 2: e54.
- 59. Faghiri Z,Skelly PJ (2009) The role of tegumental aquaporin from the human parasitic worm, Schistosoma mansoni, in osmoregulation and drug uptake. FASEB J 23: 2780–2789.
- 60. Krautz-Peterson G,Simoes M,Faghiri Z,Ndegwa D,Oliveira G,et al. (2010) Suppressing glucose transporter gene expression in schistosomes impairs parasite feeding and decreases survival in the mammalian host. PLoS Pathog 6: e1000932.
- 61. Tran MH,Freitas TC,Cooper L,Gaze S,Gatton ML,et al. (2010) Suppression of mRNAs encoding tegument tetraspanins from Schistosoma mansoni results in impaired tegument turnover. PLoS Pathog 6: e1000840.
- 62. Kumagai T,Osada Y,Ohta N,Kanazawa T (2009) Peroxiredoxin-1 from Schistosoma japonicum functions as a scavenger against hydrogen peroxide but not nitric oxide. Mol Biochem Parasitol 164: 26–31.
- 63. Kuntz AN,Davioud-Charvet E,Sayed AA,Califf LL,Dessolin J,et al. (2007) Thioredoxin glutathione reductase from Schistosoma mansoni: an essential parasite enzyme and a key drug target. PLoS Med 4: e206.
- 64. Gomes MS,Cabral FJ,Jannotti-Passos LK,Carvalho O,Rodrigues V,et al. (2009) Preliminary analysis of miRNA pathway in Schistosoma mansoni. Parasitol Int 58: 61–68.
- 65. Krautz-Peterson G,Bhardwaj R,Faghiri Z,Tararam CA,Skelly PJ (2009) RNA interference in schistosomes: machinery and methodology. Parasitology 1–11.
- 66. Krautz-Peterson G,Skelly PJ (2008) Schistosoma mansoni: the dicer gene and its expression. Exp Parasitol 118: 122–128.
- 67. Hannon GJ,Rossi JJ (2004) Unlocking the potential of the human genome with RNA interference. Nature 431: 371–378.
- 68. Jackson AL,Bartz SR,Schelter J,Kobayashi SV,Burchard J,et al. (2003) Expression profiling reveals off-target gene regulation by RNAi. Nat Biotechnol 21: 635–637.
- 69. Kulkarni MM,Booker M,Silver SJ,Friedman A,Hong P,et al. (2006) Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. Nat Methods 3: 833–838.
- 70. Ma Y,Creanga A,Lum L,Beachy PA (2006) Prevalence of off-target effects in Drosophila RNA interference screens. Nature 443: 359–363.
- 71. Sarov M,Stewart AF (2005) The best control for the specificity of RNAi. Trends Biotechnol 23: 446–448.
- 72. Sudbery I,Enright AJ,Fraser AG,Dunham I (2010) Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis. BMC Genomics 11: 175.
- 73. Fedorov Y,Anderson EM,Birmingham A,Reynolds A,Karpilow J,et al. (2006) Off-target effects by siRNA can induce toxic phenotype. RNA 12: 1188–1196.
- 74. de Moraes Mourão M,Dinguirard N,Franco GR,Yoshino TP (2009) Phenotypic screen of early-developing larvae of the blood fluke, Schistosoma mansoni, using RNA interference. PLoS Negl Trop Dis 3: e502.
- 75. Mourão MM,Dinguirard N,Franco GR,Yoshino TP (2009) Correction: phenotypic screen of early-developing larvae of the blood fluke, Schistosoma mansoni, using RNA interference. PLoS Negl Trop Dis 3:
- 76. Abdulla MH,Ruelas DS,Wolff B,Snedecor J,Lim KC,et al. (2009) Drug discovery for schistosomiasis: hit and lead compounds identified in a library of known drugs by medium-throughput phenotypic screening. PLoS Negl Trop Dis 3: e478.
- 77. Colley DG,Wikel SK (1974) Schistosoma mansoni: simplified method for the production of schistosomules. Exp Parasitol 35: 44–51.
- 78. Basch PF (1981) Cultivation of Schistosoma mansoni in vitro. I. Establishment of cultures from cercariae and development until pairing. J Parasitol 67: 179–185.
- 79. Caffrey CR,McKerrow JH,Salter JP,Sajid M (2004) Blood ‘n’ guts: an update on schistosome digestive peptidases. Trends Parasitol 20: 241–248.
- 80. Braschi S,Wilson RA (2006) Proteins exposed at the adult schistosome surface revealed by biotinylation. Mol Cell Proteomics 5: 347–356.
- 81. Tararam CA,Farias LP,Wilson RA,Leite LC (2010) Schistosoma mansoni Annexin 2: Molecular characterization and immunolocalization. Exp Parasitol 126: 146–155.
- 82. Caffrey CR,Salter JP,Lucas KD,Khiem D,Hsieh I,et al. (2002) SmCB2, a novel tegumental cathepsin B from adult Schistosoma mansoni. Mol Biochem Parasitol 121: 49–61.
- 83. Rescher U,Gerke V (2004) Annexins-unique membrane binding proteins with diverse functions. J Cell Sci 117: 2631–2639.
- 84. Shaner NC,Campbell RE,Steinbach PA,Giepmans BN,Palmer AE,et al. (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22: 1567–1572.
- 85. Rozen S,Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132: 365–386.
- 86. Nolan T,Hands RE,Bustin SA (2006) Quantification of mRNA using real-time RT-PCR. Nat Protoc 1: 1559–1582.
- 87. Bookout AL,Mangelsdorf DJ (2003) Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways. Nucl Recept Signal 1: e012.
- 88. Le TH,Blair D,Agatsuma T,Humair PF,Campbell NJ,et al. (2000) Phylogenies inferred from mitochondrial gene orders-a cautionary tale from the parasitic flatworms. Mol Biol Evol 17: 1123–1125.
- 89. Dvořák J,Mashiyama ST,Sajid M,Braschi S,Delcroix M,et al. (2009) SmCL3, a gastrodermal cysteine protease of the human blood fluke Schistosoma mansoni. PLoS Negl Trop Dis 3: e449.
- 90. Livak KJ,Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods 25: 402–408.
- 91. Yuan F,Verhelst SH,Blum G,Coussens LM,Bogyo M (2006) A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C. J Am Chem Soc 128: 5616–5617.
- 92. Murata M,Miyashita S,Yokoo C,Tamai M,Hanada K,et al. (1991) Novel epoxysuccinyl peptides. Selective inhibitors of cathepsin B, in vitro. FEBS Lett 280: 307–310.
- 93. Sojka D,Franta Z,Horn M,Hajdusek O,Caffrey CR,et al. (2008) Profiling of proteolytic enzymes in the gut of the tick Ixodes ricinus reveals an evolutionarily conserved network of aspartic and cysteine peptidases. Parasit Vectors 1: 7.
- 94. Brömme D,Klaus JL,Okamoto K,Rasnick D,Palmer JT (1996) Peptidyl vinyl sulphones: a new class of potent and selective cysteine protease inhibitors: S2P2 specificity of human cathepsin O2 in comparison with cathepsins S and L. Biochem J 315 ( Pt 1): 85–89.
- 95. Kirschke H,Shaw E (1981) Rapid interaction of cathepsin L by Z-Phe-PheCHN12 and Z-Phe-AlaCHN2. Biochem Biophys Res Commun 101: 454–458.
- 96. Krautz-Peterson G,Radwanska M,Ndegwa D,Shoemaker CB,Skelly PJ (2007) Optimizing gene suppression in schistosomes using RNA interference. Mol Biochem Parasitol 153: 194–202.
- 97. Morales FC,Furtado DR,Rumjanek FD (2004) The N-terminus moiety of the cystatin SmCys from Schistosoma mansoni regulates its inhibitory activity in vitro and in vivo. Mol Biochem Parasitol 134: 65–73.
- 98. Ndegwa D,Krautz-Peterson G,Skelly PJ (2007) Protocols for gene silencing in schistosomes. Exp Parasitol 117: 284–291.
- 99. Kiefer J,Yin HH,Que QQ,Mousses S (2009) High-throughput siRNA screening as a method of perturbation of biological systems and identification of targeted pathways coupled with compound screening. Methods Mol Biol 563: 275–287.
- 100. MacKeigan JP,Murphy LO,Blenis J (2005) Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance. Nat Cell Biol 7: 591–600.
- 101. Perrimon N,Friedman A,Mathey-Prevot B,Eggert US (2007) Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens. Drug Discov Today 12: 28–33.
- 102. Cherry S (2008) Genomic RNAi screening in Drosophila S2 cells: what have we learned about host-pathogen interactions? Curr Opin Microbiol 11: 262–270.
- 103. Prudencio M,Rodrigues CD,Hannus M,Martin C,Real E,et al. (2008) Kinome-wide RNAi screen implicates at least 5 host hepatocyte kinases in Plasmodium sporozoite infection. PLoS Pathog 4: e1000201.
- 104. Cheever AW,Weller TH (1958) Observations on the growth and nutritional requirements of Schistosoma mansoni in vitro. Am J Hyg 68: 322–339.
- 105. Clegg JA (1965) In vitro cultivation of Schistosoma mansoni. Exp Parasitol 16: 133–147.
- 106. Cousin CE,Stirewalt MA,Dorsey CH,Watson LP (1986) Schistosoma mansoni: comparative development of schistosomules produced by artificial techniques. J Parasitol 72: 606–609.
- 107. Jensen DV,Stirewalt MA,Walters M (1965) Growth of Schistosoma mansoni cercariae under dialysis membranes in Rose multipurpose chambers. Exp Parasitol 17: 15–23.
- 108. Thornhill J,Coelho PM,McVeigh P,Maule A,Jurberg AD,et al. (2009) Schistosoma mansoni cercariae experience influx of macromolecules during skin penetration. Parasitology 136: 1257–1267.