Although the acute manifestations of Chikungunya virus (CHIKV) illness are well-documented, few data exist about the long-term rheumatic outcomes of CHIKV-infected patients. We undertook between June and September 2006 a retrospective cohort study aimed at assessing the course of late rheumatic manifestations and investigating potential risk factors associated with the persistence of these rheumatic manifestations over 15 months. 147 participants (>16 yrs) with laboratory-confirmed CHIKV disease diagnosed between March 1 and June 30, 2005, were identified through a surveillance database and interviewed by telephone. At the 15-month-period evaluation after diagnosis, 84 of 147 participants (57%) self-reported rheumatic symptoms. Of these 84 patients, 53 (63%) reported permanent trouble while 31 (37%) had recurrent symptoms. Age ≥45 years (OR = 3.9, 95% CI 1.7–9.7), severe initial joint pain (OR = 4.8, 95% CI 1.9–12.1), and presence of underlying osteoarthritis comorbidity (OR = 2.9, 95% CI 1.1–7.4) were predictors of nonrecovery. Our findings suggest that long-term CHIKV rheumatic manifestations seem to be a frequent underlying post-epidemic condition. Three independent risk factors that may aid in early recognition of patients with the highest risk of presenting prolonged CHIKV illness were identified. Such findings may be particularly useful in the development of future prevention and care strategies for this emerging virus infection.
Transmitted by day-biting mosquitoes, Chikungunya virus (CHIKV), causing febrile illness and joint pain, is widespread in Africa and Asia. Recent outbreaks in the Indian Ocean islands, its rapid expansion across continents, and its unusual clinical acute pattern have shed light on and brought awareness of this re-emerging virus. However, late rheumatic manifestations of this infection remain poorly examined. Thus, we have evaluated over a 15-month period the evolution and assessed potential risk factors of CHIKV-related rheumatic manifestations in a cohort of Reunion Island residents infected with CHIKV during the initial phase of the epidemic in 2005. Eligible adult patients were identified through a surveillance database. We found that rheumatic symptoms lasted for at least 15 months in 57% of the participants. Of these, 63% reported permanent trouble while 37% had recurrent symptoms. Factors such as age ≥45 years, severe initial joint pain, and presence of underlying osteoarthritis comorbidity were associated with nonrecovery over the 15-month period. Our findings suggest that long-term CHIKV rheumatic manifestations seem to be a hidden, frequent, post-epidemic condition. These findings should be considered in the development of preventive measures.
Citation: Sissoko D, Malvy D, Ezzedine K, Renault P, Moscetti F, Ledrans M, et al. (2009) Post-Epidemic Chikungunya Disease on Reunion Island: Course of Rheumatic Manifestations and Associated Factors over a 15-Month Period. PLoS Negl Trop Dis 3(3): e389. https://doi.org/10.1371/journal.pntd.0000389
Editor: A. Desiree La Beaud, Case Western Reserve University School of Medicine, United States of America
Received: October 10, 2008; Accepted: February 5, 2009; Published: March 10, 2009
Copyright: © 2009 Sissoko et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study has been supported by the Institut de Veille Saniataire. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Chikungunya virus (CHIKV, family Togaviridae, genus Alphavirus) is a mosquito-borne virus belonging to the Semliki Forest serocomplex, which includes Ross River virus (RRV), O'nyong-nyong (ONN), Mayaro (MAY), and Barmah Forest viruses (BFV). Common clinical manifestations caused by these viruses include abrupt onset of fever, headache, backache, and arthralgia .
Since its initial identification in Newala province (Tanzania) in 1953 , CHIKV has been associated with numerous outbreaks, mainly in Africa , and Asia ,. In early 2005, CHIKV was introduced into the Southwestern Indian Ocean region, probably from infected viraemic travellers arriving from Lamu (Kenya), where an outbreak started in June 2004 . Subsequently, it rapidly spread across the Southwestern Indian Ocean islands (Comoros, Madagascar, Mayotte, Seychelles, Mauritius, Reunion Island), resulting in an extensive regional epidemic in 2005 and 2006 –. Afterward, these outbreaks' viruses expanded into Asian countries ,. Concurrently, numerous imported cases were noticed in nontropical Western countries ,. Moreover, these imported cases raised concerns about the possibility of the emergence of CHIKV infection in Europe, as Aedes albopictus, the mosquito vector of CHIKV in Reunion Island , is present in several European countries , including Italy, where it was first recorded in the 1990s and is particularly common . Indeed, this concern was born out by documentation of an autochthonous CHIKV outbreak in the Ravenna region of Italy during the summer of 2007, which was linked to a viraemic index patient originating in Kerala, India .
In Reunion Island, CHIKV outbreak evolved in two-wave phenomena with a first wave between March to June 2005 and the second one from December 2005 to June 2006. The overall attack rate in Reunion Island was estimated to be 35% in mid-2006. Subsequently, CHIKV presented with an endemo-sporadic pattern until the beginning of 2007, when it disappeared. However, in spite of this, chronic rheumatic symptoms have persisted in many previously infected individuals.
Rheumatic manifestations of CHIKV infection typically consist of a febrile arthritis principally affecting the extremities (ankles, wrists, phalanges), although many others joints may be affected . Patients adopt a characteristic stooped walking position which is the hallmark of the disease and from which it derives its name Chikungunya, meaning “he who walks bent over”, in the Kimakonde language of Mozambique. Although the acute rheumatic involvement is well-documented, less is known about the potential long-term clinical and functional outcome –. To our knowledge, the most detailed description is a cohort of 107 patients by Brighton et al. , which indicated that nearly 12% of patients had not fully recovered three years after initial infection. In that study, determinants of the course of rheumatic manifestations of CHIKV infections were not investigated.
In order to address these shortcomings, we have evaluated rheumatic complications in a cohort of Reunion Island residents infected with CHIKV during the initial phase of the epidemic in 2005. The study had two objectives, firstly to assess the course of rheumatic manifestations over the 15 months following the acute illness, and secondly to investigate potential risk factors associated with long-term outcome.
Materials and Methods
This study was conducted on Reunion Island, a French territory in the Southwest Indian Ocean. We used a community-based, retrospective cohort design involving 3,539 presumptive or laboratory-confirmed cases of CHIKV illness that were reported to the local health authority during the first wave of this outbreak (March–June 2005). Recruited persons were interviewed by telephone between June 1 and September 15, 2006.
In order to identify potentially eligible participants, we used a case notification surveillance database which has previously been described elsewhere. Patients aged more than 16 years old with serologically confirmed CHIKV infection were invited to participate. Seropositivity was defined as the presence of CHIKV-specific immunoglobulin [Ig] M antibody by IgM-capture enzyme-linked immunosorbent assay or detection of CHIKV in body fluids by amplification of CHIKV RNA using reverse transcriptase–polymerase chain reaction (RT–PCR). Other inclusion criteria included disease onset between March 1 and June 30, 2005, a telephone number accessible through the database, and provision of oral informed consent for all participants or from parents or guardians for those less than 18 years of age.
Data were collected by a trained physician using a standardised structured questionnaire in French, which was developed for the purposes of the study and administered by telephone interview.
Collected information included: demographic characteristics (age, gender, educational level, employment status, marital status), major comorbidities, acute rheumatic manifestations (anatomical location, date of onset, duration of symptoms, and pain intensity), hospitalisation, treatment received (medications, physiotherapy, alternative and complementary therapies), subjective treatment satisfaction, and impact of illness on professional or household or daily activities. Moreover, chronic rheumatic manifestations (in particular, the duration of morning stiffness of ≥45 minutes as a typical sign of inflammatory pattern) at month 15 following infection were assessed. For both acute and chronic manifestations, pain intensity was evaluated using a declarative numerical rating scale (NRS) . This scale could be scored between 0 and 10, with higher values indicating more severe pain. Scores were categorised into three classes (mild: 1–4; moderate: 5–6; or severe: 7–10).
The primary outcome measure was self-perceived recovery from rheumatic manifestations of CHIKV infections at 15 months following disease onset. This was assessed by asking two questions with bimodal (yes/no) response modes. The first question was “Do you feel that you have made a complete recovery from joint manifestations since being diagnosed as having a CHIKV infection?” For those who replied affirmatively, a subsidiary question was asked: “Over the past eight days, did symptoms of CHIKV illness subside and subsequently recur?”. This allowed permanently asymptomatic (remitted) patients to be differentiated from those who had experienced a relapse within the past eight days. Patients replying negatively to the first question and affirmatively to the second were combined in a “persistence” group. The analysis compared this “persistence” group to a “remission” group who replied negatively to the second question.
To identify potential risk factors for persistence of rheumatic manifestations, the two outcome groups were compared by univariate and multivariate unconditional logistic regression. All variables were firstly assessed individually in a univariate model, and odds ratios (OR) estimated with their corresponding 95% confidence intervals (CIs) and P-values determined with the χ2 likelihood test. Parameters whose distribution varied between groups at a probability level of P<0.25 were retained and entered into a multivariate logistic regression analysis using a forward stepwise selection procedure. Multivariate analysis was conducted with adjustment for gender. Possible interactions and multi-colinearity were examined. If two or more potential factors risk were highly correlated, the predictor that was considered to be more clinically important was selected for entry. Finally, the goodness-of-fit of the final model was assessed using the logistic regression diagnostics procedure. A probability value of ≤.05 (two-tailed) was taken to be statistically significant. Statistical analyses were performed with STATA software, version 9 (STATA Corp., College Station, Texas, United States).
In total, 3,539 presumptive or laboratory-confirmed CHIKV infection cases were reported between March 1 and June 30, 2005. CHIKV infection was confirmed serologically for 873 cases. Of these, 713 persons were excluded for the following reasons: no phone contact (n = 465); unknown date of CHIKV infection (n = 248). Of the remaining 160 patients who were invited to participate, 147 provided oral informed consent (92%) and constituted the study population.
This study population included 69% women and 31% men. The mean age of the patients was 52 years (SD: 15). Nearly 31% of the patients were age less than 45 years old, and 21% were more than 65 years old. Only 67 patients (45%) were working or studying at the time of the onset of their CHIKV illness. The median time elapsed between the onset of CHIKV illness and the interview was 439 days (range, 370 to 508 days). The most frequently encountered medical comorbidities were hypertension in 48 patients (33%), osteoarthritis in 38 (26%), and diabetes mellitus in 32 (22%) (Table 1).
Disease Characteristics and Health Care Utilisation
Table 2 presents the frequency and course of rheumatic symptoms. All 147 patients reported joint pain during the initial phase of the disease. Joint pain was reported as symmetrical in 96% of patients. The principal locations of rheumatic symptoms were the ankles in 112 (76%), the wrists in 91 (62%), the knees in 65 (44%), the fingers in 79 (54%), and the toes in 84 (57%) of the patients. More than four joints were affected (polyarthritis) in 76% of the patients.
Overall, 137 (93%) declared having been prescribed paracetamol. Among these, 106 (77%) reported a combination of paracetamol with a nonsteroid anti-inflammatory drug (NSAID). Nearly half of the patients (46%) were taking medicinal plants as complementary therapy. Corticosteroids were prescribed to 23% of patients at some stage of the disease course. Approximately 35% of patients reported being satisfied with their medication, with the exception of those taking corticosteroids, who were more satisfied (76%).
During the acute phase, 22 (15%) patients were hospitalised with a median length of stay of five days (range: 1 to 22 days).
Disease Course, Impact, and Risk Factors for Persistence
Overall, 63 (43%) declared being completely recovered at the time of the interview, while 31 (21%) reported experiencing at least one episode of recurrence, and 53 (36%) stated having permanent symptoms (Table 3). Thus, the “remission” group consisted of 63 patients and the “persistence” group included 84 individuals.
In the “persistence” group, all subjects reported the presence of joint pain, the latter symptom being associated with morning stiffness for ≥45 minutes in 73% of that group. Pain intensity was rated as mild to moderate by the majority of patients (98.8%). In this group, a reduction of daily activities as a result of their illness was reported for >90 days in 40% of subjects.
The univariate analysis comparing the “persistence “and “remission” groups is presented in Table 4. Subjects aged ≥45 years were significantly more likely to belong to the “persistence” group than those aged <45 years (OR = 4.2, 95% CI 1.9–9.3). The presence of an underlying illness was also found to be more frequent in this group (OR = 3.0, 95% CI 1.5–5.9), in particular the presence of hypertension or osteoarthritis. On the other hand, no such association was observed for other comorbidities. Finally, the severity of pain at disease onset was strongly associated with persistence (OR = 3.6, 95% CI 1.6–8.1).
In the multivariate model, three variables remained independently associated with persistence, namely age ≥45 years, initial severity of joint pain, and comorbid osteoarthritis (Table 5).
Following the massive epidemic of CHIKV infection in the Southwest Indian Ocean region in 2005–2006, local and international awareness of this condition increased markedly ,,. In previous outbreaks, acute CHIKV illness was well-documented , but chronic post-infectious conditions related to CHIKV disease had received little attention. Our community-based study reports original data on the course of chronic rheumatic manifestations among 147 patients infected during an early phase of Reunion Island's epidemic event. In this study, only 43% of patients reported full remission 15 months after acute infection. Nearly half of the patients with persistent rheumatic pain were impaired in carrying out daily or household activities for more than three months. Furthermore, our findings indicate that chronic rheumatic manifestations of CHIKV infection were independently associated with older age at the time of infection, severe initial pain, and the presence of comorbid osteoarthritis. The identification of such risk factors could be relevant for early recognition and management of patients at risk for developing persistent rheumatic symptoms.
Few previous studies have addressed the long-term clinical outcome of CHIKV illness. In a small case series of 28 residents of Pretoria with confirmed CHIKV infection, Fourie and Morrison  reported that 73% of the subjects experienced severe arthralgia in the acute phase of the illness whereas 18% reported longstanding rheumatic symptoms as long as 20 months after infection. In another South African study, Brighton et al.  reported that 12% of 107 subjects continued to experience fluctuating or persistent rheumatic manifestations three to five years after acute CHIKV illness. Compared to the present study, the lower proportion rate of persistent rheumatic manifestations may be in part related to the interval of three to five years between illness onset and the evaluation. Indeed, the prevalence of rheumatic symptoms associated with Semliki Forest serocomplex viruses, namely Ross River virus, is known to decline over time . Accordingly, it appears speculative to impute the lower prevalence principally to the population age distribution in the Brighton study. Despite this discrepancy, Brighton et al.  suggested that symptom persistence seemed more frequent in patients aged more than 40 years, consistent with the present findings. In addition, a recent study conducted on Reunion Island in the setting of a hospital-based recruitment had evaluated at month 18, 88 of 202 (44%) CHIKV-infected patients who fulfill the inclusion criteria . Among these, 56 patients (63.6%) reported persistent arthralgia, with 29 (51.8% of the 56) ascertaining a history of arthralgia before CHIKV illness. As noted by the authors, these results are of interest for the influence of a previous history of rheumatic manifestations in the course of persistent arthralgia in patients with CHIKV infection. However, with regard to the very low rate of evaluated patients among those recruited, a meaningful selection bias had probably induced an overestimation of the proportion of persistent arthralgia. Moreover, it can be hypothesized that the patients who had totally recovered from CHIKV illness are less likely to comply and participate in such a followup study.
Our observation of symptom persistence in 57% of subjects is reminiscent of findings from longitudinal studies of rheumatic manifestations of other alphaviral infections reported from Australia (RRV) , and Finland (Sindbis virus, SINV) ,, although others have pointed out that other confounding factors may influence the duration of rheumatic symptoms in such diseases . Hence, we recognize it can be very challenging to differentiate between osteoarthritis and Chikungunya-related arthralgia. This fact has been highlighted by evaluations conducted through clinical series of CHIKV-infected patients which clearly identified a wide range of rheumatologic manifestations including tenosynovitis and lesions involving previously injured bones or joints . Nonetheless, the presence of osteoarthritis conditions before CHIKV infection appears to be an independent risk factor for developing late rheumatism manifestations. Thus, further investigations should elucidate the pathophysiologic patterns underlying this relationship.
With respect to symptom management, an important finding was that only one-third of patients reported being satisfied with the most prescribed class of treatment (i.e., NSAIDs). On the other hand, treatment satisfaction with corticosteroids at any stage of the disease was considerably higher. However, it should be emphasised that caution should be exercised when using corticosteroids in such patients (infected with a virus). Nonetheless, the study demonstrates that in practice, treatment of rheumatic symptoms of CHIKV illness was generally inadequate. However, the role of inadequate treatment as a potential risk factor for persistence could not be evaluated in the present study. This issue would be worth addressing in future studies.
This study has certain limitations that should be taken into consideration when interpreting the results. Firstly, even if the participation rate was high, only one-fifth of the eligible subjects were invited to participate since telephone contact was not available for the other subjects. Additionally, subjects under 16 years old were not eligible for the study for the reason that they are generally less likely to answer a phone-standardized questionnaire. Therefore, these findings may not be generalizable to all cases of CHIKV infection that occurred on Reunion Island. Secondly, data were collected by self-report, which precluded symptom ascertainment by objective clinical examination. Moreover, with subjective self-report, recall bias leading to underestimation of either current or past symptoms cannot be excluded. Finally, this evaluation was conducted while the second wave of the outbreak was ongoing in 2006 ; in this context, the constitution of a CHIKV antibody negative controls group for the setting of a case-control study design appeared irrelevant. This restriction is a break for the estimation of long-term absolute rheumatic-morbidity attributable to CHIK-illness. These limitations, however, should be tempered, as retrospective studies allow giving a rapid and prompt response to health authorities by providing consistent and relevant information and tools that will help to adapt delivered messages to the population and the medical staff in the continuing context of Reunion Island's longstanding outbreak.
The results of this study highlight the fact that, following the CHIKV outbreak in 2005 on Reunion Island, a substantial proportion of persistent and disabling residual rheumatic symptoms could be identified for at least 15 months after infection onset, especially in individuals ≥45 years of age. We also identified two other independent risk factors associated with persistence of rheumatic symptoms that may be particularly useful in the development and improvement of future prevention and care strategies for this emerging viral infection. Our findings also draw attention to the importance of assessing management issues such as strategies for supportive treatment of CHIK illness in further studies. Finally, these studies should be designed in order to estimate the magnitude of chronic rheumatic illness directly attributable to CHIKV infection and its potential effect on quality of life over a prolonged period.
We are grateful to the patients who agreed to participate in this study. We thank Elsa Balleydier and Agnes Cadivel for preparing the surveillance and survey databases. We are also indebted to Laurent Filleul (Cire Aquitaine) and Henriette Devalk (InVS, Saint Maurice) for their skillful help during study conception.
Conceived and designed the experiments: DS PR FM ML VP. Analyzed the data: DS DM KE VP. Wrote the paper: DS DM KE PR FM ML VP.
- 1. Tesh RB (1982) Arthritides caused by mosquito-borne viruses. Annu Rev Med 33: 31–40.RB Tesh1982Arthritides caused by mosquito-borne viruses.Annu Rev Med333140
- 2. Robinson MC (1955) An epidemic of virus disease in Southern Province, Tanganyika Territory, in 1952–53. I. Clinical features. Trans R Soc Trop Med Hyg 49: 28–32.MC Robinson1955An epidemic of virus disease in Southern Province, Tanganyika Territory, in 1952–53. I. Clinical features.Trans R Soc Trop Med Hyg492832
- 3. Thonnon J, Spiegel A, Diallo M, Diallo A, Fontenille D (1999) [Chikungunya virus outbreak in Senegal in 1996 and 1997]. Bull Soc Pathol Exot 92: 79–82.J. ThonnonA. SpiegelM. DialloA. DialloD. Fontenille1999[Chikungunya virus outbreak in Senegal in 1996 and 1997].Bull Soc Pathol Exot927982
- 4. Pastorino B, Muyembe-Tamfum JJ, Bessaud M, Tock F, Tolou H, et al. (2004) Epidemic resurgence of Chikungunya virus in democratic Republic of the Congo: Identification of a new central African strain. J Med Virol 74: 277–282.B. PastorinoJJ Muyembe-TamfumM. BessaudF. TockH. Tolou2004Epidemic resurgence of Chikungunya virus in democratic Republic of the Congo: Identification of a new central African strain.J Med Virol74277282
- 5. Lam SK, Chua KB, Hooi PS, Rahimah MA, Kumari S, et al. (2001) Chikungunya infection—An emerging disease in Malaysia. Southeast Asian J Trop Med Public Health 32: 447–451.SK LamKB ChuaPS HooiMA RahimahS. Kumari2001Chikungunya infection—An emerging disease in Malaysia.Southeast Asian J Trop Med Public Health32447451
- 6. Mackenzie JS, Chua KB, Daniels PW, Eaton BT, Field HE, et al. (2001) Emerging viral diseases of Southeast Asia and the Western Pacific. Emerg Infect Dis 7: 497–504.JS MackenzieKB ChuaPW DanielsBT EatonHE Field2001Emerging viral diseases of Southeast Asia and the Western Pacific.Emerg Infect Dis7497504
- 7. Sergon K, Njuguna C, Kalani R, Ofula V, Onyango C, et al. (2008) Seroprevalence of Chikungunya Virus (CHIKV) Infection on Lamu Island, Kenya, October 2004. Am J Trop Med Hyg 78: 333–337.K. SergonC. NjugunaR. KalaniV. OfulaC. Onyango2008Seroprevalence of Chikungunya Virus (CHIKV) Infection on Lamu Island, Kenya, October 2004.Am J Trop Med Hyg78333337
- 8. Sergon K, Yahaya AA, Brown J, Bedja SA, Mlindasse M, et al. (2007) Seroprevalence of Chikungunya virus infection on Grande Comore Island, union of the Comoros, 2005. Am J Trop Med Hyg 76: 1189–1193.K. SergonAA YahayaJ. BrownSA BedjaM. Mlindasse2007Seroprevalence of Chikungunya virus infection on Grande Comore Island, union of the Comoros, 2005.Am J Trop Med Hyg7611891193
- 9. Renault P, Solet JL, Sissoko D, Balleydier E, Larrieu S, et al. (2007) A major epidemic of chikungunya virus infection on Reunion Island, France, 2005–2006. Am J Trop Med Hyg 77: 727–731.P. RenaultJL SoletD. SissokoE. BalleydierS. Larrieu2007A major epidemic of chikungunya virus infection on Reunion Island, France, 2005–2006.Am J Trop Med Hyg77727731
- 10. Sissoko D, Moendandze A, Malvy D, Giry C, Ezzedine K, et al. (2008) Seroprevalence and risk factors of chikungunya virus infection in Mayotte, Indian Ocean, 2005–2006: A population-based survey. PLoS ONE 3: e3066.D. SissokoA. MoendandzeD. MalvyC. GiryK. Ezzedine2008Seroprevalence and risk factors of chikungunya virus infection in Mayotte, Indian Ocean, 2005–2006: A population-based survey.PLoS ONE3e3066
- 11. Beesoon S, Funkhouser E, Kotea N, Spielman A, Robich RM (2008) Chikungunya Fever, Mauritius, 2006. Emerg Infect Dis 14: 337–338.S. BeesoonE. FunkhouserN. KoteaA. SpielmanRM Robich2008Chikungunya Fever, Mauritius, 2006.Emerg Infect Dis14337338
- 12. Ratsitorahina M, Harisoa J, Ratovonjato J, Biacabe S, Reynes JM, et al. (2008) Outbreak of dengue and Chikungunya fevers, Toamasina, Madagascar, 2006. Emerg Infect Dis 14: 1135–1137.M. RatsitorahinaJ. HarisoaJ. RatovonjatoS. BiacabeJM Reynes2008Outbreak of dengue and Chikungunya fevers, Toamasina, Madagascar, 2006.Emerg Infect Dis1411351137
- 13. Yergolkar PN, Tandale BV, Arankalle VA, Sathe PS, Sudeep AB, et al. (2006) Chikungunya outbreaks caused by African genotype, India. Emerg Infect Dis 12: 1580–1583.PN YergolkarBV TandaleVA ArankallePS SatheAB Sudeep2006Chikungunya outbreaks caused by African genotype, India.Emerg Infect Dis1215801583
- 14. Powers AM, Logue CH (2007) Changing patterns of chikungunya virus: Re-emergence of a zoonotic arbovirus. J Gen Virol 88: 2363–2377.AM PowersCH Logue2007Changing patterns of chikungunya virus: Re-emergence of a zoonotic arbovirus.J Gen Virol8823632377
- 15. Krastinova E, Quatresous I, Tarantola A (2006) Imported cases of chikungunya in metropolitan France: Update to June 2006. Euro Surveill 11: E060824.E. KrastinovaI. QuatresousA. Tarantola2006Imported cases of chikungunya in metropolitan France: Update to June 2006.Euro Surveill11E060824
- 16. Lanciotti RS (2007) Chikungunya Virus in US Travelers Returning from India, 2006. Emerg Infect Dis 13: 764–767.RS Lanciotti2007Chikungunya Virus in US Travelers Returning from India, 2006.Emerg Infect Dis13764767
- 17. Reiter P, Fontenille D, Paupy C (2006) Aedes albopictus as an epidemic vector of chikungunya virus: Another emerging problem? Lancet Infect Dis 6: 463–464.P. ReiterD. FontenilleC. Paupy2006Aedes albopictus as an epidemic vector of chikungunya virus: Another emerging problem?Lancet Infect Dis6463464
- 18. Gratz NG (2004) Critical review of the vector status of Aedes albopictus. Med Vet Entomol 18: 215–227.NG Gratz2004Critical review of the vector status of Aedes albopictus.Med Vet Entomol18215227
- 19. Romi R, Di Luca M, Majori G (1999) Current status of Aedes albopictus and Aedes atropalpus in Italy. J Am Mosq Control Assoc 15: 425–427.R. RomiM. Di LucaG. Majori1999Current status of Aedes albopictus and Aedes atropalpus in Italy.J Am Mosq Control Assoc15425427
- 20. Rezza G, Nicoletti L, Angelini R, Romi R, Finarelli AC, et al. (2007) Infection with chikungunya virus in Italy: An outbreak in a temperate region. Lancet 370: 1840–1846.G. RezzaL. NicolettiR. AngeliniR. RomiAC Finarelli2007Infection with chikungunya virus in Italy: An outbreak in a temperate region.Lancet37018401846
- 21. Kennedy AC, Fleming J, Solomon L (1980) Chikungunya viral arthropathy: A clinical description. J Rheumatol 7: 231–236.AC KennedyJ. FlemingL. Solomon1980Chikungunya viral arthropathy: A clinical description.J Rheumatol7231236
- 22. Fourie ED, Morrison JG (1979) Rheumatoid arthritic syndrome after chikungunya fever. S Afr Med J 56: 130–132.ED FourieJG Morrison1979Rheumatoid arthritic syndrome after chikungunya fever.S Afr Med J56130132
- 23. Jeandel P, Josse R, Durand JP (2004) [Exotic viral arthritis: Role of alphavirus]. Med Trop (Mars) 64: 81–88.P. JeandelR. JosseJP Durand2004[Exotic viral arthritis: Role of alphavirus].Med Trop (Mars)648188
- 24. Brighton SW, Prozesky OW, de la Harpe AL (1983) Chikungunya virus infection. A retrospective study of 107 cases. S Afr Med J 63: 313–315.SW BrightonOW ProzeskyAL de la Harpe1983Chikungunya virus infection. A retrospective study of 107 cases.S Afr Med J63313315
- 25. Jones KR, Vojir CP, Hutt E, Fink R (2007) Determining mild, moderate, and severe pain equivalency across pain-intensity tools in nursing home residents. J Rehabil Res Dev 44: 305–314.KR JonesCP VojirE. HuttR. Fink2007Determining mild, moderate, and severe pain equivalency across pain-intensity tools in nursing home residents.J Rehabil Res Dev44305314
- 26. Mavalankar D, Shastri P, Raman P (2007) Chikungunya epidemic in India: A major public-health disaster. Lancet Infect Dis 7: 306–307.D. MavalankarP. ShastriP. Raman2007Chikungunya epidemic in India: A major public-health disaster.Lancet Infect Dis7306307
- 27. Pialoux G, Gauzere BA, Jaureguiberry S, Strobel M (2007) Chikungunya, an epidemic arbovirosis. Lancet Infect Dis 7: 319–327.G. PialouxBA GauzereS. JaureguiberryM. Strobel2007Chikungunya, an epidemic arbovirosis.Lancet Infect Dis7319327
- 28. Harley D, Bossingham D, Purdie DM, Pandeya N, Sleigh AC (2002) Ross River virus disease in tropical Queensland: Evolution of rheumatic manifestations in an inception cohort followed for six months. Med J Aust 177: 352–355.D. HarleyD. BossinghamDM PurdieN. PandeyaAC Sleigh2002Ross River virus disease in tropical Queensland: Evolution of rheumatic manifestations in an inception cohort followed for six months.Med J Aust177352355
- 29. Borgherini G, Poubeau P, Jossaume A, Gouix A, Cotte L, et al. (2008) Persistent arthralgia associated with chikungunya virus: A study of 88 adult patients on Reunion Island. Clin Infect Dis 47: 469–475.G. BorgheriniP. PoubeauA. JossaumeA. GouixL. Cotte2008Persistent arthralgia associated with chikungunya virus: A study of 88 adult patients on Reunion Island.Clin Infect Dis47469475
- 30. Westley-Wise VJ, Beard JR, Sladden TJ, Dunn TM, Simpson J (1996) Ross River virus infection on the North Coast of New South Wales. Aust N Z J Public Health 20: 87–92.VJ Westley-WiseJR BeardTJ SladdenTM DunnJ. Simpson1996Ross River virus infection on the North Coast of New South Wales.Aust N Z J Public Health208792
- 31. Condon RJ, Rouse IL (1995) Acute symptoms and sequelae of Ross River virus infection in South-Western Australia: A follow-up study. Clin Diagn Virol 3: 273–284.RJ CondonIL Rouse1995Acute symptoms and sequelae of Ross River virus infection in South-Western Australia: A follow-up study.Clin Diagn Virol3273284
- 32. Laine M, Luukkainen R, Jalava J, Ilonen J, Kuusisto P, et al. (2000) Prolonged arthritis associated with sindbis-related (Pogosta) virus infection. Rheumatology (Oxford) 39: 1272–1274.M. LaineR. LuukkainenJ. JalavaJ. IlonenP. Kuusisto2000Prolonged arthritis associated with sindbis-related (Pogosta) virus infection.Rheumatology (Oxford)3912721274
- 33. Kurkela S, Manni T, Myllynen J, Vaheri A, Vapalahti O (2005) Clinical and laboratory manifestations of Sindbis virus infection: Prospective study, Finland, 2002–2003. J Infect Dis 191: 1820–1829.S. KurkelaT. ManniJ. MyllynenA. VaheriO. Vapalahti2005Clinical and laboratory manifestations of Sindbis virus infection: Prospective study, Finland, 2002–2003.J Infect Dis19118201829
- 34. Mylonas AD, Brown AM, Carthew TL, McGrath B, Purdie DM, et al. (2002) Natural history of Ross River virus-induced epidemic polyarthritis. Med J Aust 177: 356–360.AD MylonasAM BrownTL CarthewB. McGrathDM Purdie2002Natural history of Ross River virus-induced epidemic polyarthritis.Med J Aust177356360
- 35. Simon F, Parola P, Grandadam M, Fourcade S, Oliver M, et al. (2007) Chikungunya infection: An emerging rheumatism among travelers returned from Indian Ocean islands. Report of 47 cases. Medicine (Baltimore) 86: 123–137.F. SimonP. ParolaM. GrandadamS. FourcadeM. Oliver2007Chikungunya infection: An emerging rheumatism among travelers returned from Indian Ocean islands. Report of 47 cases.Medicine (Baltimore)86123137