In the United States, there is a largely hidden burden of diseases caused by a group of chronic and debilitating parasitic, bacterial, and congenital infections known as the neglected infections of poverty. Like their neglected tropical disease counterparts in developing countries, the neglected infections of poverty in the US disproportionately affect impoverished and under-represented minority populations. The major neglected infections include the helminth infections, toxocariasis, strongyloidiasis, ascariasis, and cysticercosis; the intestinal protozoan infection trichomoniasis; some zoonotic bacterial infections, including leptospirosis; the vector-borne infections Chagas disease, leishmaniasis, trench fever, and dengue fever; and the congenital infections cytomegalovirus (CMV), toxoplasmosis, and syphilis. These diseases occur predominantly in people of color living in the Mississippi Delta and elsewhere in the American South, in disadvantaged urban areas, and in the US–Mexico borderlands, as well as in certain immigrant populations and disadvantaged white populations living in Appalachia. Preliminary disease burden estimates of the neglected infections of poverty indicate that tens of thousands, or in some cases, hundreds of thousands of poor Americans harbor these chronic infections, which represent some of the greatest health disparities in the United States. Specific policy recommendations include active surveillance (including newborn screening) to ascertain accurate population-based estimates of disease burden; epidemiological studies to determine the extent of autochthonous transmission of Chagas disease and other infections; mass or targeted treatments; vector control; and research and development for new control tools including improved diagnostics and accelerated development of a vaccine to prevent congenital CMV infection and congenital toxoplasmosis.
Citation: Hotez PJ (2008) Neglected Infections of Poverty in the United States of America. PLoS Negl Trop Dis 2(6): e256. doi:10.1371/journal.pntd.0000256
Editor: Simon Brooker, London School of Hygiene & Tropical Medicine, United Kingdom
Published: June 25, 2008
Copyright: © 2008 Peter J. Hotez. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The author received no specific funding for this study.
Competing interests: PJH is Executive Director of the Global Network for Neglected Tropical Disease Control (GNNTDC), the Director of the Human Hookworm Vaccine Initiative (HHVI), and President of the Sabin Vaccine Institute. He is an inventor on an international patent for a hookworm vaccine. The author recused himself from all editorial decisions regarding this paper.
In the United States of America, the mortality rate resulting from infectious diseases has declined precipitously over the course of the twentieth century , and major scourges such as typhoid fever and malaria are no longer serious public health threats . However, among the poorest populations living in the US there remains highly prevalent a group of serious parasitic and bacterial diseases such as Chagas disease, cysticercosis, and toxocariasis , which, like the neglected tropical diseases (NTDs), are characterized by their high prevalence, chronic and disabling features, and disproportionate effect on the poor ,. These infections occur outside of tropical regions of Africa, Asia, and Latin America, and I refer to them as neglected infections of poverty, because they not well known to the US public-health community, and they promote poverty because of their impact on child development, pregnancy outcomes, and worker productivity . In this review I highlight the largely underappreciated burden of the neglected infections of poverty in the US and make policy recommendations for addressing such health disparities.
The Distressed Regions of Poverty in the United States
Demographers and other social scientists measure poverty in a number of ways ,, but since the 1960s, the US Census Bureau has used a set of income thresholds that vary by family size and composition ,. In 2006, there were 36.5 million Americans living in poverty, and the official US poverty rate was 12.3% ,. However, among under-represented minorities and children, the poverty rate is much higher, particularly in single-parent households headed by women (Table 1). Poverty in the US is not evenly distributed, but instead it is focally concentrated into several defined geographic regions, each with unique socioeconomic characteristics. Glasmeier has identified six major distressed regions of poverty: Appalachia, the Mississippi Delta, other areas of rural poverty especially in the American South, Native American tribal lands, the borderlands between the United States and Mexico, and highly racially segregated urban areas including mostly black metro areas adjacent to the Great Lakes and in the Northeast . Holt has conducted a spatial analysis of the poverty in the United States at the county level and independently identified similar areas of poverty (Figure 1) .
Top: Counties south of the Continental Divide. Bottom: Counties north of the Continental Divide. From Holt .
A robust dataset links poverty to both lower life expectancies from chronic diseases (especially cancer and heart disease) and increased infant and child mortality –. Partly on this basis, and building on an analysis of mortality by race and ethnicity in 2,077 counties or county clusters, Murray et al. divided the US population into eight groups with different epidemiologic patterns and mortality rates . Among these eight “Americas” were four socioeconomically disadvantaged groups with substantially higher mortality from chronic diseases: America 4 is defined as poor whites living in Appalachia and the Mississippi Valley; America 5, Native Americans living on reservations in the West; America 7, poor blacks living in the rural South; and America 8, blacks living in high-risk urban environments .
Using a hybrid of these classifications it is possible to identify groups of individuals based on race, ethnicity, and socioeconomic status that are at particular risk for specific neglected infections of poverty. In this paper I review the prevalence of the major neglected diseases of poverty in the US This analysis was conducted in January 2008 using the online database PubMed  for 1972–2007 with the Medical Subject Headings (MSHs) “neglected diseases”, “poverty”, the specific geographic regions and racial, ethnic, and socioeconomic groups listed above ,,, and the specific diseases listed as NTDs on the PLoS Neglected Tropical Diseases journal scope page , as well as major congenital infections associated with impaired child development including cytomegalovirus (CMV) infection, toxoplasmosis, and syphilis. I also reviewed reference lists of identified articles and hand-searched reviews. I report here either previously published estimates of the number of cases of each neglected infection, or I provide a range of estimates based on reported prevalence rates among selected communities multiplied by published estimates of the population at risk having similar socioeconomic, racial, and ethnic demographics (Table 2). For some neglected infections, particularly the soil-transmitted helminth infections, no new surveys have been reported since the 1980s. Some of the regional and national prevalence estimates were modified from a chapter in my recently published book on neglected tropical diseases .
The hilly and mountainous region known as Appalachia comprises parts of 13 states (Figure 1) . Poverty and isolation is particularly severe in Central Appalachia, which includes parts of West Virginia, Eastern Kentucky and Tennessee, and the southwestern tip of Virginia . The plight of the poorest people in this region, typically those working in the coal mining industry, was brought to national attention both during the early 1960s when John F. Kennedy made a presidential campaign swing through the region  and with the 1962 publication of Michael Harrington's book, The Other America: Poverty in the United States . In 2000, it was estimated that 169,000 housing units in Appalachia, particularly Central Appalachia, had no indoor plumbing . Almost 3% of the region overall lacks complete plumbing, although in some counties plumbing is incomplete in upwards of 25% of the housing units .
The parasitic worm infection ascariasis is one of the world's most common neglected tropical diseases , and a leading global cause of impaired child development . In very young children, high-intensity Ascaris lumbricoides infections also cause intestinal obstruction ,. During the 1930s, the profound poverty and inadequate sanitation in Appalachia was linked to high rates of ascariasis . For instance, it was noted that among children aged 5–14 y, the prevalence in Breathitt County in Eastern Kentucky was 75%, higher than in many developing countries . During the late 1970s Walzer et al. reported that approximately 14% of schoolchildren in Clay County (Eastern Kentucky) were infected with A. lumbricoides and almost 13% were also infected with the whipworm Trichuris trichiura , while other investigators also reported that ascariasis was still highly endemic in the region –. Warren previously estimated that four million people are infected with A. lumbricoides in the US (Table 2) : however, no surveys for ascariasis have since been conducted.
Strongyloidiasis, caused by the threadworm Strongyloides stercoralis, is another important soil-transmitted helminth infection, associated with chronic enteritis, impaired child development, eosinophilia, and hyperinfection in immunocompromised hosts –. The disease is under-reported partly because of the difficulty of diagnosing the infection by fecal examination ,. A review of several studies conducted during the 1960s, 70s, and 80s and determined that the prevalence in Central Appalachia ranged from 0.4% (Charleston, West Virginia) to 4.0% (Harlan County, Kentucky, and Johnson City, Tennessee) . Based on 3,271 fecal examinations in Kentucky, Walzer et al. estimated that the overall prevalence was approximately 1% . A high percentage of the patients with strongyloidiasis were found to be older white males, most of whom had underlying chronic illnesses including chronic obstructive pulmonary disease ,,,. These infections may have been acquired in coal mines. Murray et al. determined that 11 million people compose the poor white Appalachians in America 4 , while the Centers for Disease Control and Prevention (CDC) reported that the population of rural Appalachia is approximately 6.8 million . Based on Walzer's prevalence determination of 1%, I estimate there are approximately 68,000  to 110,000 Appalachians infected with S. stercoralis (Table 2).
Mississippi Delta and the American South
Throughout the twentieth century and continuing today, the Mississippi Delta (“the Delta,” composed predominantly of the Delta regions of Mississippi, Louisiana, Arkansas, and Tennessee, but also including the adjacent “boot-heel” of Missouri) and the areas of the former Cotton Belt in the American South, have remained among the poorest regions in the nation (Figure 1) . High rural poverty rates, inadequate housing, and poor health are the hallmarks of poverty in the Delta and adjacent regions ,. More than one-half of the population is black , and over one-third of the total Delta black population lives in poverty, as does almost one-half of the rural black Delta population . Overall, 5.8 million people live in America 7, the poor blacks of the rural American South .
In the first half of the twentieth century as many as 42% of black schoolchildren in the Delta had splenomegaly indicative of active malaria infection, and almost twice as many blacks died from malaria as whites . The high rates of malaria among blacks were attributed to exposure to Anopheles mosquitoes as a result of crowding and inadequate housing located next to swampy land, and diminished host resistance because of malnourishment and overwork . Throughout the American South during the early twentieth century, malaria combined with hookworm infection and pellagra to produce a generation of anemic, weak, and unproductive children and adults ,,,. By the 1960s these infections were no longer endemic in the Delta, but the health status (as measured by cancer and heart disease mortality rates and infant mortality rates) of the eight states that make up the this region still consistently ranks at the bottom among all the United States . Tuberculosis rates among southern blacks are also considerably higher than whites –. Poverty is a major determinant but not the only one , as incarceration and other involuntary social forces also account for high rates of tuberculosis and some sexually transmitted infections , –. For the blacks living in the Delta and elsewhere in the American South, several parasitic and congenital infections rank among the most important neglected infections of poverty, especially in post-Katrina Louisiana.
Neglected infections in pre- and post-Katrina Louisiana.
Despite the apparent eradication of malaria and hookworm infection from the American South ,,,, other important parasitic infections remain, particularly in Louisiana. Even before Hurricane Katrina, the Delta region of Louisiana exhibited some of the highest poverty rates in the nation—in 2000, approximately 36% of blacks lived below the poverty level in this area . It was previously determined that, outside of Appalachia, Louisiana exhibited some of the highest rates of ascariasis in the US , and during the 1970s and 1980s considerable numbers of the rural residents of Louisiana and elsewhere in the American South were infected , –. Some children exhibited parasite intensities high enough to produce acute intestinal obstruction ,. Although A. lumbricoides infections were highest in rural Louisiana, they were also prevalent among kindergarten children living in New Orleans . In addition, during the 1970s and 1980s Louisiana children were at risk for infection with the dog roundworm, Toxocara canis , and up to 30% of rural black children, mostly in the South, were seropositive for this infection (toxocariasis will be discussed in the section on inner cities) . Unfortunately, no surveys for either ascariasis or toxocariasis in Louisiana have been published since the 1980s.
Following the devastation of Hurricane Katrina in 2005, prolonged flooding combined with poverty to create conditions that could promote the emergence of additional neglected infections, including vector-borne viral diseases such as dengue fever – and Chagas disease ,. Chagas disease is of particular concern, because of the noted rise in domestic triatomines, especially Triatoma sanguisuga, which transmits the causative American trypanosome Trypanosoma cruzi ,. In Louisiana, almost 30% of the armadillos and 38% of the opossums are infected with T. cruzi, and a case of Chagas disease was recently reported in post-Katrina New Orleans . Therefore, many of the requirements for autochthonous Chagas disease transmission are in place in Louisiana , with an established case already present. In the coming decade, global warming and increased flooding in the region could combine to promote dengue and Chagas disease epidemics among the poor in Louisiana .
The feminization of poverty.
The term “feminization of poverty” refers to the observation that in the US and elsewhere women often have fewer economic resources than do men and are more likely to be heads of single-parent families . Poverty is particularly feminized among black women . As shown in Table 1, almost one-half of black female heads of single-parent households live below the poverty level, and black mothers are twice as likely to have premature or low birth weight infants or to have infants that die in infancy than white mothers . Congenital infections, typically the result of primary cytomegalovirus (CMV) infection, toxoplasmosis, or syphilis during pregnancy, are important factors underlying these high rates of poor birth outcome. These congenital infections cause devastating long-term neurological dysfunction including cognitive impairments, intellectual retardation, and hearing and vision loss –. In this way, the major congenital infections are also important poverty-promoting factors causing billions of dollars in economic losses . In the US, black children and their mothers bear a disproportionate congenital disease burden . With respect to congenital CMV, black women exhibit a 4-fold increase in primary infection during pregnancy compared to white women, and when stratified for women between the ages of 12 and 19 there is almost a 50-fold increase . Of the estimated 27,002 primary CMV infections in pregnancy in the US estimated to occur annually, 6,652 of them occur in black women (Table 2) . Similarly, almost 55% of the cases of congenital syphilis occur among blacks , and blacks suffer from higher rates of toxoplasmosis than do whites (Table 2) . In addition to primary infections during pregnancy and congenital infections, black women also exhibit an approximately 10-fold higher prevalence of trichomoniasis (13.3%) than white (1.3%) women . Based on Murray's estimate that 13.3 million blacks live either in America 7 (rural South) and in America 8 (high-risk urban environments ), I estimate that approximately 880,000 black women in the US are infected with the protozoan parasite Trichomonas vaginalis (Table 2).
Disadvantaged Urban Enclaves (Inner Cities)
High-poverty areas in American inner cities are sometimes defined as neighborhoods where more than 40% of the population is poor . Jargowsky described such neighborhoods as ones that “tend to have a threatening appearance marked by dilapidated housing, vacant units with broken or boarded up windows, abandoned or burned out cars, and men ‘hanging out’ on street corners” . One measure of inner city poverty used by sociologists and economists is a dissimilarity index, which measures the degree of segregation by race and income, with blacks living in the poorest neighborhoods . The cities with the highest dissimilarity index are the Northeastern cities and the Midwestern cities near the Great Lakes (Figure 1) . Several neglected infections are present in these and other disadvantaged urban enclaves.
Rat-borne and louse-borne bacterial infections.
Over the last two decades, outbreaks of leptospirosis, a bacterial infection transmitted through rat urine and responsible for a serious hemorrhagic complication known as Weil's disease, have been reported among the poor living in Baltimore  and Detroit ,. Similarly, bartonellosis, caused by the gram-negative bacterium Bartonella quintana, has emerged among the homeless –. B. quintana is the cause of louse-borne trench fever, so named because it was common among soldiers living under extreme conditions in the trenches during World War I ,. Beginning in the 1990s, small outbreaks of B. quintana bacteremia and endocarditis was noted among the homeless living in Seattle, Washington, and elsewhere –. With global warming and increased flooding such rat- and louse-borne infections may increase among the homeless .
Toxocariasis is an important neglected infection of poverty among socioeconomically disadvantaged black children ,,. Playgrounds and sandboxes in poor urban neighborhoods are often contaminated with eggs of the dog roundworm, Toxocara canis ,. When children accidentally ingest these roundworms eggs the released larvae migrate through tissues to cause visceral larval migrans and eosinophilic granuloma of the liver ,, or ocular larva migrans ,. Another form of the disease, covert toxocariasis, has been associated with asthma ,,, and may possibly be linked to the rise in asthma observed in inner city children , as well as impaired cognitive development and lower intelligence ,,. Based on serologic studies that measure antibody to T. canis antigens, the prevalence rate of toxocariasis among inner city blacks living in Connecticut cities was found to be 10% and even higher among inner city Hispanics . As noted previously, the prevalence among socioeconomically disadvantaged blacks in the American South was as high as 30% . In an unpublished study from the CDC it was recently estimated that approximately 21% of blacks are seropositive ( and Peter Schantz, personal communication), indicating exposure to the parasite. I previously estimated that approximately 500,000 blacks are seropositive for T. canis antibody . However, based on the estimate that 13.3 million impoverished blacks live in America 7 and 8  and prevalence estimates between 10% and 21%, as many as 1.3 million to 2.8 million individuals may be exposed or infected (Table 2).
African Refugees and Other Special Immigrant Groups
Since the 1980s, the US has relocated and successfully treated populations of refugees from Southeast Asia and other developing regions with high prevalence rates of helminth infections—especially hookworm infection, filarial infections, and strongyloidiasis –—tuberculosis, and hepatitis B . Beginning in 2000, the immigration of refugees from sub-Saharan Africa markedly increased , and today the US settles an estimated 70,000 refugees annually, including 25,000 refugees from Africa . Notable among the refugees are the “Lost Boys and Girls of Sudan,” raised initially in poor Ethiopian refugee camps before relocating to Kenya . Since 2000, almost 4,000 Lost Boys and Girls have been settled in the US. By serologic testing it was determined that almost one-half of these special immigrants are seropositive for both schistosomiasis (mostly Schistosoma mansoni infection) and strongyloidiasis . In addition, an estimated 8,000 Somali Bantu have been relocated to the US, with up to three-fourths of them seropositive for schistosomiasis (most likely Schistosoma haematobium infection) and one-fourth positive for strongyloidiasis . It is generally accepted that seropositivity for these two parasitic infections is a result of chronic and persistent untreated infections . Therefore, of the roughly 4,000 Sudanese immigrants and 8,000 Somali immigrants there are approximately 8,000 cases of schistosomiasis and 3,000 cases of strongyloidiasis (Table 2). Accordingly, the CDC now recommends presumptive treatment for these special immigrant populations with anthelminthics –.
The Borderlands of Mexico
An estimated 10 million people live in the border region between the US and Mexico, many of whom are of Hispanic heritage (the majority American citizens) (Figure 1) . These border communities are among the poorest in the US, and substandard or inadequate housing is common to the region . Several important neglected infections of poverty occur in this setting, including vector-borne diseases, helminth infections, and other zoonoses. A related at-risk population is the estimated 750,000 to 12 million migrant farm laborers from Mexico and Central America .
Vector-borne diseases: Dengue, Chagas disease, and leishmaniasis.
Poor housing without plumbing, air conditioning, or window screens is a key factor in promoting vector-borne diseases . It has been estimated that this situation describes more than 30,000 border households, in addition to large numbers of mobile homes in the region . Over the 20-y period between 1980 and 1999 there were 65,514 cases of dengue fever reported from the Mexico side of the border, compared to only 64 cases in the US ,,. An earlier assessment suggested that the higher-quality dwellings on the US side accounted for this disparity ; however, more recent studies indicate that dengue is under-reported in the US near the Mexican border . A cross-sectional survey in Brownsville, Texas and Matamoros Tamaulipas, Mexico detected 2% and 7.3% recent infections, respectively, with evidence of past infection in 40% of Brownsville residents . Risk factors and predictors of dengue among the Brownsville residents include low weekly family income, absence of air conditioning, and inadequate street drainage . Assuming that 10 million people live in the US–Mexico borderlands, a 2% prevalence of recent infections  translates to approximately 200,000 people with recent dengue fever (Table 2). Alternatively, the Pew Hispanic Center estimates that there are 26,784,268 Mexican Americans living in the US . At an overall poverty rate of 20.6% for Hispanics in the US (Table 1), there are almost six million impoverished Mexican Americans in the US. If 2% of this population suffers from a recent dengue infection, I estimate there are 110,000 recent dengue infections in the US (Table 2).
In addition to evidence for Chagas disease in post-Katrina Louisiana as described above, the US borderlands with Mexico have also emerged as an endemic region –. Because of concerns about the risk of new contamination of the national blood supply with T. cruzi ,,,, with a recent estimate that between 1 in 4,655 and 1 in 25,000 US blood donors are seropositive for T. cruzi antibodies and presumed infected ,, there is great interest in expanding current blood screening efforts . In 2006, the US Food and Drug Administration approved a new commercial ELISA test for blood donation screening that utilizes parasite lysate antigens for detection of antibodies ,. Estimates of the prevalence of Chagas disease along the Mexico border and in the US vary widely. Previously, it was estimated that 50,000 to 100,000 Latin American immigrants in the US are infected , but more recently it was found that of 10,192 blood specimens from El Paso, Texas, of which 73% were from donors of Hispanic origin, three donors were positive . With an overall prevalence of 0.03%  and 10 million people living in the US–Mexico borderlands , I estimate that approximately 3,000 people have Chagas disease in the region. Other estimates are considerably higher. Milei et al. argued that there are 370,000 T. cruzi–infected individuals in the US during the 1990s , while Hanford et al. revised these estimates to suggest that over one million Hispanics in the US have Chagas disease (with almost 270,000 in Texas alone) and that at least 150,000 Latin America–born immigrants are expected to develop clinically apparent chronic Chagas disease . Congenital Chagas disease may also occur ,. Of particular concern is the possibility that T. cruzi transmission to humans today occurs in the US–Mexico borderlands. In South Texas and elsewhere along the US–Mexico borderlands, dogs and coyotes are seropositive and there is a domestic canine transmission cycle . In addition, wood rats are common hosts, and the infection occurs among domestic cattle, horses, and sheep . Infected vectors or hosts are present in 64 of the 254 counties in Texas , so people living in the estimated 30,000 poor-quality dwellings in the borderlands region are at high risk for transmission.
Another vector-borne neglected disease, cutaneous leishmaniasis, is transmitted by sandflies and is endemic in Mexico and Central America. Infection with Leishmania mexicana has been reported from South Texas, including among individuals with no travel history ,; wood rats or other rodents may also serve as reservoir hosts.
Cysticercosis and other zoonoses.
Cysticercosis results when humans accidentally ingest eggs of the pork tapeworm, Taenia solium, which are shed or excreted by close household or family contacts. This condition is now a leading cause of epilepsy, seizures, and other neurological sequelae in the US–Mexico borderlands –, accounting for approximately 10% of seizures presenting to emergency rooms in Los Angeles and, presumably, other border cities as well . With an incidence rate of 8 to 10 per 100,000 per year among Hispanic populations ,, I previously estimated that up to 3,500 new cases of cysticercosis occur annually . In a seroprevalence study of rural Ventura County, California, it was found that 1.8% of that population have cysticercosis ,. I previously reported that there are 41,400 Hispanics in the US with cysticercosis , but based on the observation that 9.4 million Hispanics live in poverty in the US , the number of people with cysticercosis may be substantially higher. If 1.8% of this population is also infected, there may be as many as 169,000 cases of cysticercosis among Hispanics in the US (Table 2).
There are two other zoonoses of medical importance in the US–Mexico borderlands. Brucellosis is one of the most common zoonosis worldwide and a leading cause of disability . Goat and cow dairy products are an important source of infection from Mexico , with 1,056 cases of brucellosis reported between 1993 and 2002 (although Mead et al. estimated that 1,554 cases occur annually ), of which almost 80% of the cases occur among individuals of Hispanic origin . Between 1994 and 2000, 129 cases of bovine tuberculosis (Mycobacterium bovis) were reported, nearly all among patients of Hispanic origin, particularly children .
Neglected infections among migrant farm workers.
Approximately 95% of the several million migrant agricultural workers in the US were born in Mexico, and almost all of them live below the poverty line . They have significant health disparities, with case fatality rates more than five times the US average. In addition to very high rates of HIV, tuberculosis, and chronic diseases , –, the Mexican-born migrant workers living in the US often suffer from high rates of parasitic infection, including ascariasis and hookworm infection , – (for which there is evidence of autochthonous transmission on US farms ), cysticercosis and Chagas disease ,, and other neglected infections ,.
Tribal Lands and Arctic Native Americans
Approximately 4 million Native Americans are distributed among 500 tribes in the United States, with approximately one-fourth living on tribal lands or lands specifically designated as Native American lands (Figure 1) . Almost 30% of those living on tribal lands live in poverty, where the child poverty rates are more than 40% .
Neglected infections in continental US tribal lands.
Across the US, Native Americans are highly susceptible to diabetes mellitus and obesity, and almost one-third of Native Americans die before the age of 45 . Up to 40% of Native Americans also live in overcrowded conditions , and because of this and for additional reasons of genetic susceptibility and low vaccine coverage, high rates of invasive bacterial and viral respiratory infections occur among Native Americans, especially the Navajo and Apache –. On some reservations up to one in five homes lack complete in-house plumbing, a rate that is 20 times the national average . In this setting, certain neglected infections of poverty are common. Over the last twenty years in the American Southwest, trachoma has been common among the Navajo ,, while cystic echinococcosis has been endemic among the Navajo, Zuni, and Santo Domingo Indians because of an enzootic dog–sheep cycle on tribal lands and elsewhere in the region –.
Neglected infections among the Inuit.
Because of their dietary reliance on meat from sea mammals and polar bear the Inuit living in Alaska and the Canadian Arctic are at risk of food-borne parasitic diseases, including echinococcosis, toxoplasmosis and congenital toxoplasmosis, and trichinellosis . Cystic echinococcosis in the Arctic is due to an enzootic cycle involving moose, reindeer, and elk , while trichinellosis caused by Trichinella spiralis nativa is prevalent because of high rates of infection among walruses and polar bear . Toxoplasmosis and congenital toxoplasmosis are also extremely common among the Inuit, and are due to consumption of infected seal and caribou meat .
The most diagnosed parasitic in the infection in the US is giardiasis ,, with as many as 2.0–2.5 million cases occurring annually ,. The greatest number of cases occurs between June and October and among children aged 1–4 and 5–9 y and adults aged 35–39 y . An estimated 300,000 cases of cryptosporidiosis also occur annually , and this infection has emerged as a leading cause of recreational water outbreaks of diarrhea in the US and among patients with HIV/AIDS . A 10-fold increase in cryptosporidiosis transmission occurs during the summer and early fall . Although both giardiasis and cryptosporidiosis are common, there is no evidence to suggest that they disproportionately affect poor and under-represented minority populations. In contrast, the intestinal protozoan disease amebiasis does disproportionately affect the poor, but no US prevalence data are available for this disease. Among the notifiable neglected infections of poverty there were 166 cases of leprosy (with most of the cases in Texas, California, New York and Louisiana ), 16 cases of trichinellosis, and two cases of human rabies reported in 2005 (Table 2) .
Based on my estimates of prevalence (Table 2) and other health and socioeconomic impacts, the most important neglected helminth infections of poverty in the US are the helminth diseases toxocariasis (inner cities and the American South), ascariasis (Appalachia and the American South), strongyloidiasis (Appalachia), and cysticercosis (US–Mexico borderlands). Among the important vector-borne neglected infections are dengue and Chagas disease in the US–Mexico borderlands and in post-Katrina Louisiana. Congenital infections such as congenital CMV and congenital syphilis stand out as health disparities in inner cities and the American South. Trench fever and leptospirosis are important among the homeless and other disadvantaged urban populations.
Among the common features of these neglected infections are (1) their highly disproportionate health impact on people of color and people living in poverty; (2) their chronic, largely insidious, and disabling features; and (3) their ability to promote poverty because of their impact on child development, pregnancy outcome, and productive capacity. It is important to note that, while some of these neglected infections occur exclusively among recent immigrant populations, most do not. Instead, poverty is the single most important determinant. Control of these neglected infections needs to be prioritized by policy makers and public-health experts because it is both a highly cost-effective mechanism for lifting disadvantaged populations out of poverty and consistent with our shared American values of equity and equality . The World Health Organization also recognizes that control of neglected diseases represents a fundamental human right .
An important obstacle to the control or elimination of the neglected infections of poverty in the US is the absence of reliable population-based estimates of prevalence and disease burden data about these conditions ,. These neglected infections are underdiagnosed and most are not reportable to the CDC. The estimates I provide here are preliminary and based on very few active surveillance studies, including some obtained by analyses of sera collected from National Health and Examination Surveys. For some of the neglected infections of poverty, seropositivity may be equated with active infection ,,,,, whereas for others it may reflect both current and past infections ,. For infections such as Chagas disease estimates reported here vary widely. We also lack a system for the national collection of fecal samples for intestinal parasitic infections. Expanded measures are urgently needed to implement active surveillance and obtain population-based estimates of the neglected infections (Table 3). An added measure would be to expand newborn screening for toxoplasmosis ,, and possibly congenital Chagas disease. Screening for congenital toxoplamosis would also likely benefit persons of all socioeconomic circumstances . Such efforts would create opportunities to determine the extent and true disease burden of these neglected infections.
There is also an urgent need to better define the transmission dynamics of some of the neglected diseases (Table 3). For Chagas disease, and to some extent, dengue and leishmaniasis, the full extent of authochthonous transmission in Louisiana and the US–Mexico borderlands is poorly understood. A full appreciation of Chagas disease transmission mechanisms would include molecular genotyping of the parasite to determine whether different strains or demes are endemic, and a complete characterization of the different vectors and animal reservoir hosts. Similarly, the extent of autochthonous cysticercosis transmission in the US is largely unstudied, as it is for many of the bacterial zoonoses including urban foci of leptospirosis and trench fever. For toxocariasis, the contribution of feral versus domesticated animal reservoirs to transmission is also not well understood.
Following enhanced surveillance and improved understanding of transmission dynamics, there are several opportunities to treat or prevent neglected infections of poverty in the US using existing drugs or other control tools (Table 3). Through either population-based drug administration or case identification and treatment, the soil-transmitted helminths could be controlled by administration of albendazole and ivermectin , while expanded use of praziquantel would treat schistosomiasis among selected immigrant populations  and prevent transmission of T. solium eggs and possibly reduce the incidence of cysticercosis . Metronidazole and tinidazole are available for the treatment of trichomoniasis and giardiasis ,, and nitazoxanide is available for cryptosporidiosis and giardiasis ,. Pyrimethamine plus sulfadiazine is used for the treatment of toxoplasmosis, and the optimal length of treatment and its impact on child development and neurological sequelae need to be determined . Antibiotics are available for the treatment of leptospirosis and other bacterial zoonoses . An important role also exists for veterinary public health interventions to prevent zoonotic transmission to humans, possibly including the mass treatment of Toxocara-infected dogs, Toxoplasma-infected cats, and other measures . The control of almost all of the neglected infections of poverty would also benefit from improvements in environmental sanitation, piped clean water, and improvements in housing in some of the poorest endemic areas. For Chagas disease, dengue, and leishmaniasis, consideration of expanded vector control approaches is warranted ,.
Development of new control and prevention tools is needed (Table 3). Currently, the serologic-based diagnostic tests for most of the parasitic infections rely on extracts or crude preparations of parasite antigens and would benefit from the development of improved and widely available diagnostic kits that utilize standardized and purified recombinant antigens. For Chagas disease there is a particularly urgent need for rapid diagnostic tests and polymerase chain reaction-based assays for detection of acute and congenital infections. Furthermore, no drugs adequately and reliably treat Chagas disease , dengue , or congenital CMV infection . Although vaccines for dengue  and CMV infection  are under development, progress has been slow because of inadequate resources and commercial incentives . A pediatric dengue vaccine initiative was recently established through support by the Gates Foundation . For CMV infection, both a live attenuated vaccine and a recombinant vaccine have been developed , but clinical testing in pregnant women to determine the impact of these vaccines on vertical transmission has been severely lagging because of inadequate support—a tragedy, given that more than 10,000 congenital CMV infections occur among infants of color annually .
In 2006, the annual budget of the National Institute of Allergy and Infectious Diseases (NIAID) was $4.4 billion, with approximately $1.6 billion of this amount spent on biodefense . Of the selected disease-specific areas targeted for funding by the NIAID in their published annual report, none specifically mentions a neglected infection of poverty . A consequence of this lack of targeted funding for neglected diseases is that the development of critically needed new tools for these conditions has lagged behind those for biodefense. The Global Forum on Health Research has coined the term “the 10/90 gap” to describe how only 10% of resources are devoted to 90% of the global burden of disease, i.e., that represented by disease disproportionately occurring in developing countries . The absence of development of new tools for neglected infections of poverty, such as those outlined above, highlights a unique American 10/90 gap for poor people and people of color in the US.
I wish to thank Drs. Mark Eberhard and Peter Schantz from the US Centers for Disease Control and Prevention for their helpful discussions and insights.
- 1. Armstrong GL, Conn LA, Pinner RW (1999) Trends in infectious disease mortality in the United States during the 20th century. JAMA 281: 61–66.
- 2. Humphreys M (2001) Malaria: Poverty, Race, and Public Health in the United States. Baltimore and London: Johns Hopkins University Press. pp. 49–68.
- 3. Hotez PJ (2007) Neglected diseases and poverty in “The Other America”: The greatest health disparity in the United States? PLoS Negl Trop Dis 1: e159. doi:10.1371/journal.pntd.0000149.
- 4. Hotez PJ, Molyneux DH, Fenwick A, Kumaresan J, Ehrlich Sachs S, et al. (2007) Control of neglected tropical diseases. N Engl J Med 357: 1018–27.
- 5. Hotez PJ, Ferris MT (2006) The antipoverty vaccines. Vaccine 24: 5787–99.
- 6. Iceland J (2006) Poverty in America: A Handbook. Second Edition. Berkeley, Los Angeles, London: University of California Press. pp. 20–37.
- 7. Rector RE, Johnson KA (2004) Understanding poverty in America, Backgrounder #1713. Washington (D. C.): Heritage Foundation. Available: http://www.heritage.org/Research/Welfare/bg1713.cfm. Accessed 16 February, 2008.
- 8. (2007) Poverty. Definitions. US Census Bureau. Available: http://www.census.gov/hhes/www/poverty/definitions.html. Accessed 16 February 2008.
- 9. (2007) Poverty: 2006 highlights. US Census Bureau. Available: http://www.census.gov/hhes/www/poverty/poverty06/pov06hi.html. Accessed 16 February 2008.
- 10. (2007) Historical poverty tables. Table 4. Available: http://www.census.gov/hhes/www/poverty/histpov/hstpov4.html. Accessed 16 February 2008.
- 11. Glasmeier AK (2006) An Atlas of Poverty in America: One Nation, Pulling Apart, 1960–2003. Distressed Regions Section. New York and London: Routledge Taylor & Francis Group. pp. 51–80.
- 12. Holt JB (2007) The topography of poverty in the United States: A spatial analysis using county-level data from the community health status indicators project. Preventing Chronic Disease: Public Health Research, Practice, and Policy. Available: http://cdc.gov/pcd/issues/2007/oct/07_0091.htm. Accessed 12 January 2008.
- 13. Bloche G (2007) Opening presentation. Health Status Disparities in the United States [conference paper]. Woodrow Wilson International Center for Scholars Conference. 4 April 2007. Available: http://www.wilsoncenter.org/topics/docs/Bloche.pdf. Accessed 12 January 2008.
- 14. Murray CJL, Kulkarni S, Ezzati M (2005) Eight Americas. New perspectives on U.S. health disparities. Am J Prev Med 29: 4–10.
- 15. Kaplan G (2007) Socioeconomic Inequalities in Health: What they look like and what can be done about them [conference paper]. Health Status Disparities in the United States. Woodrow Wilson International Center for Scholars Conference. 4 April 2007. Available: http://www.wilsoncenter.org/topics/docs/Kaplan.pdf. Accessed 12 January 2008.
- 16. Braveman P (2007) The unsolved mystery of racial disparities in infant health: Do we know enough to act? [conference paper]. Health Status Disparities in the United States. Woodrow Wilson International Center for Scholars Conference. 4 April 2007. Available: http://www.wilsoncenter.org/topics/docs/Braveman.pdf. Accessed 12 January 2008.
- 17. US National Library of Medicine, National Institutes of Health (2008) PubMed. Available: http://www.ncbi.nlm.nih.gov/sites/entrez/. Accessed 12 January 2008.
- 18. (2008) PLoS Neglected Tropical Diseases journal scope. Public Library of Science. Available: http://www.plosntds.org/static/scope.action. Accessed 12 January 2008.
- 19. Hotez PJ (2008) Forgotten People and Forgotten Diseases. American Society of Microbiology Press.
- 20. Mangum GL, Mangum SL, Sum AM (2003) The Persistence of Poverty in the United States. Baltimore and London: Johns Hopkins University Press. pp. 1–11.
- 21. Harrington M (1962) The Other America: Poverty in the United States. New York: Macmillan.
- 22. Bethony J, Brooker S, Albonico M, Geiger SM, Loukas A, et al. (2006) Soil-transmitted helminth infections: Ascariasis, trichuriasis, and hookworm. Lancet 367: 1521–32.
- 23. Blumenthal DS, Schultz MG (1975) Incidence of intestinal obstruction in children infected with Ascaris lumbricoides. Am J Trop Med Hyg 24: 801–805.
- 24. Otto GF, Cort WW (1934) The distribution and epidemiology of human ascariasis in the United States. Am J Epidemiol 19: 657–712.
- 25. Walzer PD, Milder JE, Banwell JG, Kilgore G, Klein M, et al. (1982) Epidemiologic features of Strongyloides stercoralis infection in an endemic area of the United States. Am J Trop Med Hyg 31: 313–319.
- 26. Jones JE (1983) Parasites in Kentucky: the past seven decades. J Kentucky Med Assoc 81: 621–626.
- 27. Dauer CC, Korns RF, Schulman LM (1968) Infectious Diseases. Cambridge (Massachusetts): Harward University Press. pp. 113–118.
- 28. Blumenthal DS (1977) Intestinal nematodes in the United States. N Engl J Med 297: 1437–1439.
- 29. Warren KS (1974) Helminthic diseases endemic in the United States. Am J Trop Med Hyg 23: 723–730.
- 30. Milder JE, Walzer PD, Kilgore G, Rutherford I, Klein M (1981) Clinical features of Strongyloides stercoralis infection in an endemic area of United States. Gastroenterology 80: 1481–1488.
- 31. Dada-Adegbola HO, Bakare RA (2004) Strongyloidiasis in children five years and below. West Afr J Med 23: 194–197.
- 32. Siddiqui AA, Berk SL (2001) Diagnosis of Strongyloides stercoralis infection. Clinical Infectious Diseases 33: 1040–7.
- 33. Kitchen LW, Tu KK, Kerns FT (2000) Strongyloides-infected patients at charleston area medical center, West Virginia, 1997–1998. Clin Infect Dis 31: E5–6.
- 34. Berk SL, Verghese A, Alvarez S, Hall K, Smith B (1987) Clinical and epidemiologic features of Strongyloidiasis. A prospective study in rural Tennessee. Arch Intern Med 147(7): 1257–1261.
- 35. Centers for Disease Control and Prevention (2002) Lung cancer death rates in Appalachia, 1994–1998. MMWR Morb Mortal Wkly Rep 51: 527–529.
- 36. Felix H, Stewart MK (2005) Health status in the Mississippi River delta region. South Med J 98: 149–154.
- 37. McKinnon J (2001) The black population: 2000. Census 2000 brief. US Census Bureau. Available: http://www.census.gov/prod/2001pubs/c2kbr01-5.pdf. Accessed 16 February 2008.
- 38. Martin MG, Humphreys ME (2006) Social consequence of disease in the American South, 1900–world war II. South Med J 99: 862–864.
- 39. Bleakley H (2007) Disease and development: evidence from hookworm eradication in the American South. Q J Econ 122: 73–112.
- 40. Centers for Disease Control and Prevention (2004) Racial disparities in tuberculosis - selected Southeastern States, 1991–2002. MMWR Morb Mortal Wkly Rep 53: 556–559.
- 41. Acevedo-Garcia D (2000) Residential segregation and the epidemiology of infectious diseases. Soc Sci Med 51: 1143–1161.
- 42. Richardus JH, Kunst AE (2001) Black-white differences in infectious disease mortality in the United States. Am J Public Health 91: 1251–1253.
- 43. Thomas JC (2006) From slavery to incarceration: Social forces affecting the epidemiology of sexually transmitted diseases in the rural south. Sex Transm Dis 33: S6–10.
- 44. Thomas JC, Torrone E (2006) Incarceration as forced migration: effects on selected community health outcomes. Am J Public Health 96: 1762–1765.
- 45. Martin LK (1972) Hookworm in Georgia. I. Survey of intestinal helminth infections and anemia in rural school children. Am J Trop Med Hyg 21: 919–929.
- 46. Adams RT, Perkin JE (1985) The prevalence of intestinal parasites in children living in an unincorporated area in rural Northern Florida. J Sch Health 55: 76–78.
- 47. Morgan PM, Hubbard DW, Willis RA, Unglaub WG, Langham RA, et al. (1972) Intestinal parasitism and nutritional status in Louisiana. J La State Med Soc 124: 197–203.
- 48. Blumenthal DS, Schultz MG (1976) Effects of Ascaris infection of nutritional status in children. Am J Trop Med Hyg 25: 682–690.
- 49. Miller MJ, Farahmandian I, Arfaa F, Katz N, Winsor E, et al. (1978) An evaluation of levamisole for treatment of ascariasis. South Med J 71: 137–140.
- 50. Schultz MG (1982) Ascariasis: Nutritional implications. Rev Infect Dis 4: 815–819.
- 51. Hubbard DW, Morgan PM, Yaeger RG, Unglaub WG, Hood MW, et al. (1974) Intestinal parasite survey of kindergarten children in New Orleans. Pediatr Res 8: 652–658.
- 52. Smith RE, Hagstad HV, Beard GB (1984) Visceral larva migrans: a risk assessment in Baton Rouge, Louisiana. Int J Zoonoses 11: 189–194.
- 53. Herrmann N, Glickman LT, Schantz PM, Weston MG, Domanski LM (1985) Seroprevalence of zoonotic toxocariasis in the United States: 1971–1973. Am J Epidemiol 122: 890–896.
- 54. Moore CG, Francy DB, Eliason DA, Monath TP (1988) Aedes albopictus in the United States: rapid spread of a potential disease vector. J Am Mosq Control Assoc 4: 356–361.
- 55. Gubler DJ, Reiter P, Ebi KL, Yap W, Nasci R, et al. (2001) Climate variability and change in the United States: potential impacts on vector- and rodent-borne diseases. Environ Health Perspect 109: Suppl 2223–233.
- 56. Morens DM, Fauci AS (2008) Dengue and hemorrhagic fever: A potential threat to public health in the United States. JAMA 299: 214–216.
- 57. Dorn PL, Perniciaro L, Yabsley MJ, Roellig DM, Balsamo G, et al. (2007) Autochthonous transmission of trypanosoma cruzi, Louisiana. Emerg Infect Dis 13: 605–607.
- 58. Diaz JH (2007) Chagas disease in the United States: A cause for concern in Louisiana? J La State Med Soc 159: 21–3, 25–9.
- 59. Starrels M, Bould S, Nicholas LJ (1994) The feminization of poverty in the United States. J Family Issues 15: 590–607.
- 60. Prober CG, Enright AM (2003) Congenital cytomegalovirus (CMV) infections: Hats off to Alabama. J Pediatr 143: 4–6.
- 61. McLeod R, Boyer K, Karrison T, Kasza K, Swisher C, et al. (2006) Outcome of treatment for congenital toxoplasmosis, 1981–2004: The National Collaborative Chicago-based, Congenital Ttoxoplasmosis Study. Clin Infect Dis 42: 1383–1394.
- 62. Centers for Disease Control and Prevention (2002) Congenital syphilis - United States. MMWR Morb Mortal Wkly Rep 53: 716–719.
- 63. Staras SA, Dollard SC, Radford KW, Flanders WD, Pass RF, et al. (2006) Seroprevalence of cytomegalovirus infection in the United States, 1988–1994. Clin Infect Dis 43: 1143–1151.
- 64. Colugnati FA, Staras SA, Dollard SC, Cannon MJ (2007) Incidence of cytomegalovirus infection among the general population and pregnant women in the United States. BMC Infect Dis 7: 71.
- 65. Jones JL, Kruszon-Moran D, Sanders-Lewis K, Wilson M (2007) Toxoplasma gondii infection in the United States, 1999–2004, decline from the prior decade. Am J Trop Med Hyg 77: 405–410.
- 66. Sutton M, Sternberg M, Koumans EH, McQuillan G, Berman S, et al. (2007) The prevalence of Trichomonas vaginalis infection among reproductive-age women in the United States, 2001–2004. Clin Infect Dis 45: 1319–1326.
- 67. Jargowsky PA (1997) Poverty and place: Ghettos, barrios, and the American city. New York: Russell Sage Foundation.
- 68. Vinetz JM, Glass GE, Flexner CE, Mueller P, Kaslow DC (1996) Sporadic urban leptospirosis. Ann Intern Med 125: 794–798.
- 69. Thiermann AB, Frank RR (1980) Human leptospirosis in Detroit and the role of rats as chronic carriers. Int J Zoonoses 7: 62–72.
- 70. Demers RY, Frank R, Demers P, Clay M (1985) Leptospiral exposure in Detroit rodent control workers. Am J Public Health 75: 1090–1091.
- 71. Spach DH, Kanter AS, Dougherty MJ, Larson AM, Coyle MB, et al. (1995) Bartonella (rochalimaea) quintana bacteremia in inner-city patients with chronic alcoholism. N Engl J Med 332: 424–428.
- 72. Jackson LA, Spach DH, Kippen DA, Sugg NK, Regnery RL, et al. (1996) Seroprevalence to Bartonella quintana among patients at a community clinic in downtown Seattle. J Infect Dis 173: 1023–1026.
- 73. Brouqui P, Raoult D (2006) Arthropod-borne diseases in homeless. Ann N Y Acad Sci 1078: 223–235.
- 74. Despommier D (2003) Toxocariasis: Clinical aspects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev 16: 265–272.
- 75. Sharghi N, Schantz P, Hotez PJ (2000) Toxocariasis: An occult cause of childhood neuropsychological deficits and asthma? Semin Pediatr Infect Dis 11: 257–260.
- 76. Chorazy ML, Richardson DJ (2005) A survey of environmental contamination with ascarid ova, Wallingford, Connecticut. Vector Borne Zoonotic Dis 5: 33–39.
- 77. Kaplan KJ, Goodman ZD, Ishak KG (2001) Eosinophilic granuloma of the liver: A characteristic lesion with relationship to visceral larva migrans. Am J Surg Pathol 25: 1316–1321.
- 78. Stewart JM, Cubillan LD, Cunningham ET Jr (2005) Prevalence, clinical features, and causes of vision loss among patients with ocular toxocariasis. Retina 25: 1005–1013.
- 79. Sharghi N, Schantz P, Caramico L, Ballas K, Teague BA, et al. (2001) Environmental exposure to toxocara as a possible risk factor for asthema: A clinic-based case-control study. Clin Infect Dis 32: e111–6.
- 80. Buijs J, Borsboom G, Renting M, Hilgersom WJ, van Wieringen JC, et al. (1997) Relationship between allergic manifestations and toxocara seropositivity: A cross-sectional study among elementary school children. Eur Respir J 10: 1467–1475.
- 81. Busse WW, Mitchell H (2007) Addressing issues of asthma in inner-city children. J Allergy Clin Immunol 119: 43–49.
- 82. Nelson S, Greene T, Ernhart CB (1996) Toxocara canis infection in preschool age children: Risk factors and the cognitive development of preschool children. Neurotoxicol Teratol 18: 167–174.
- 83. Marmor M, Glickman L, Shofer F, Faich LA, Rosenberg C, et al. (1987) Toxocara canis infection of children: Epidemiologic and neuropsychologic findings. Am J Public Health 77: 554–559.
- 84. Won K, Kruzon-Moran D, Schantz PM, Jones JL (2007) National seroprevalence and risk factors for Toxocara spp. infection. Am J Trop Med Hyg 77: 68.
- 85. Nutman TB, Ottesen EA, Ieng S, Samuels J, Kimball E, et al. (1987) Eosinophilia in Southeast Asian refugees: Evaluation at a referral center. J Infect Dis 155: 309–313.
- 86. Garg PK, Perry S, Dorn M, Hardcastle L, Parsonnet J (2005) Risk of intestinal helminth and protozoan infection in a refugee population. Am J Trop Med Hyg 73: 386–391.
- 87. Seybolt LM, Christiansen D, Barnett ED (2006) Diagnostic evaluation of newly arrived asymptomatic refugees with eosinophilia. Clin Infect Dis 42: 363–367.
- 88. Barnett ED (2004) Infectious disease screening for refugees resettled in the United States. Clin Infect Dis 39: 833–41.
- 89. Franco-Paredes C, Dismukes R, Nicolls D, Hidron A, Workowski K, et al. (2007) Persistent and untreated tropical infectious diseases among Sudanese refugees in the United States. Am J Trop Med Hyg 77: 633–5.
- 90. Posey DL, Blackburn BG, Weinberg M, Flagg EW, Ortega L, et al. (2007) High prevalence and presumptive treatment of Schistosomiasis and Strongyloidiasis among African refugees. Clin Infect Dis 45: 1310–5.
- 91. Miller JM, Boyd HA, Ostrowski SR, Cookson ST, Parise ME, et al. (2000) Malaria, intestinal parasites, and schistosomiasis among barawan somali refugees resettling to the United States: A strategy to reduce morbidity and decrease the risk of imported infections. Am J Trop Med Hyg 62: 115–21.
- 92. Holmes SM (2006) An ethnographic study of the social context of migrant health in the United States. PLoS Med 3: e448. doi: 10.1371/journal.pmed.0030448.
- 93. Reiter P, Lathrop S, Bunning M, Biggerstaff B, Singer D, et al. (2003) Texas lifestyle limits transmission of dengue virus. Emerg Infect Dis 9: 86–9.
- 94. Reiter P (2001) Climate change and mosquito-borne disease. Environ Health Perspect 109: (Suppl 1)141–61.
- 95. Brunkard JM, Robles López JL, Ramirez J, Cifuentes E, Rothenberg SJ, et al. (2007) Dengue fever seroprevalence and risk factors, Texas-Mexico border, 2004. Emerg Infect Dis 13: 1477–83.
- 96. Pew Hispanic Center (2008) A statistical portrait of Hispanics at mid-decade. Available: http://pewhispanic.org/reports/middecade/. Accessed 1 February 2008.
- 97. Beard CB, Pye G, Steurer FJ, Rodriguez R, Campman R, et al. (2003) Chagas disease in a domestic transmission cycle, Southern Texas, USA. Emerg Infect Dis 9: 103–5.
- 98. Bern C, Montgomery SP, Herwaldt BL, Rassi A Jr, Marin-Neto JA, et al. (2007) Evaluation and treatment of Chagas disease in the United States: A systematic review. JAMA 298: 2171–81.
- 99. Centers for Disease Control and Prevention (2006) Chagas disease after organ transplantation - Los Angeles, California. MMWR Morb Mortal Wkly Rep 55: 798–800.
- 100. Centers for Disease Control and Prevention (2007) Blood donor screening for Chagas disease - United States 2006–2007. MMWR Morb Mortal Wkly Rep 56: 141–3.
- 101. Dodd RY, Leiby DA (2004) Emerging infectious threats to the blood supply. Annu Rev Med 55: 191–207.
- 102. Hanford EJ, Zhan FB, Lu Y, Giordano A (2007) Chagas disease in Texas: Recognizing the significance and implications of evidence in the literature. Soc Sci Med 65: 60–79.
- 103. Leiby DA, Herron RM Jr, Read EJ, Lenes BA, Stumpf RJ (2002) Trypanosoma cruzi in Los Angeles and Miami blood donors: impact of evolving donor demographics on seroprevalence and implications for transfusion transmission. Transfusion 42: 549–55.
- 104. Leiby DA, Rentas FJ, Nelson KE, Stambolis VA, Ness PM, et al. (2000) Evidence of Trypanosoma cruzi infection (Chagas' disease) among patients undergoing cardiac surgery. Circulation 102: 2978–82.
- 105. Milei J, Mautner B, Storino R, Sanchez JA, Ferrans VJ (1992) Does Chagas' disease exist as an undiagnosed form of cardiomyopathy in the United States? Am Heart J 123: 1732–5.
- 106. Navin TR, Roberto RR, Juranek DD, Limpakarnjanarat K, Mortenson EW, et al. (1985) Human and sylvatic Trypanosoma cruzi infection in California. Am J Public Health 75: 366–9.
- 107. Rassi A Jr, Rassi A, Little WC (2000) Chagas' heart disease. Clin Cardiol 23: 883–9.
- 108. Tarleton RL, Reithinger R, Urbina JA, Kitron U, Gürtler RE (2007) The challenges of Chagas disease–grim outlook or glimmer of hope. PLoS Med 4: e332. doi:10.1371/journal.pmed.0040332.
- 109. Tobler LH, Contestable P, Pitina L, Groth H, Shaffer S, et al. (2007) Evaluation of a new enzyme-linked immunosorbent assay for detection of Chagas antibody in US blood donors. Transfusion 47: 90–6.
- 110. Muñoz J, Portús M, Corachan M, Fumadó V, Gascon J (2007) Congenital trypanosoma cruzi infection in a non-endemic area. Trans R Soc Trop Med Hyg 101: 1161–2.
- 111. Enserink M (2000) Infectious diseases. Has leishmaniasis become endemic in the U.S.? Science 290: 1881–3.
- 112. Maloney DM, Maloney JE, Dotson D, Popov VL, Sanchez RL (2002) Cutaneous leishmaniasis: Texas case diagnosed by electron microscopy. J Am Acad Dermatol 47: 614–6.
- 113. DeGiorgio C, Pietsch-Escueta S, Tsang V, Corral-Leyva G, Ng L, et al. (2005) Sero-prevalence of Taenia solium cysticercosis and Taenia solium taeniasis in California, USA. Acta Neurol Scand 111: 84–8.
- 114. DeGiorgio CM, Sorvillo F, Escueta SP (2005) Neurocysticercosis in the United States: Review of an important emerging infection. Neurology 64: 1486.
- 115. del la Garza Y, Graviss EA, Daver NG, Gambarin KJ, Shandera WX, et al. (2005) Epidemiology of neurocysticercosis in Houston, Texas. Am J Trop Med Hyg 73: 766–70.
- 116. Ong S, Talan DA, Moran GJ, Mower W, Newdow M, et al. (2002) Neurocysticercosis in radiographically imaged seizure patients in U.S. emergency departments. Emerg Infect Dis 8: 608–13.
- 117. Shandera WX, White AC Jr, Chen JC, Diaz P, Armstrong R (1994) Neurocysticercosis in Houston, Texas. A report of 112 cases. Medicine (Baltimore) 73: 37–52.
- 118. Sorvillo FJ, DeGiorgio C, Waterman SH (2007) Deaths from cysticercosis, United States. Emerg Infect Dis 13: 230–235.
- 119. Wallin MT, Kurtzke JF (2004) Neurocysticercosis in the United States: Review of an important emerging infection. Neurology 63: 1559–1564.
- 120. White AC Jr, Atmar RL (2002) Infections in Hispanic immigrants. Clin Infect Dis 34: 1627–1632.
- 121. Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV (2006) The new global map of human brucellosis. Lancet Infect Dis 6: 91–99.
- 122. Troy SB, Rickman LS, Davis CE (2005) Brucellosis in San Diego: Epidemiology and species-related differences in acute clinical presentations. Medicine (Baltimore) 84: 174–187.
- 123. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, et al. (1999) Food-related illness and death in the United States. Emerg Infect Dis 5: 607–625.
- 124. LoBue PA, Betacourt W, Peter C, Moser KS (2003) Epidemiology of Mycobacterium bovis disease in San Diego County, 1994–2000. Int J Tuberc Lung Dis 7: 180–185.
- 125. Centers for Disease Control and Prevention (1992) Prevention and control of tuberculosis in migrant farm workers. Recommendations of the Advisory Council for the Elimination of Tuberculosis. MMWR Recomm Rep 41(RR-10): 1–15.
- 126. Poss JE (1998) The meanings of tuberculosis for Mexican migrant farm workers in the United States. Soc Sci Med 47: 195–202.
- 127. Villarejo D (2003) The health of U.S. hired farm workers. Annu Rev Public Health 24: 175–193.
- 128. Bechtel GA (1998) Parasitic infections among migrant farm families. J Community Health Nurs 15: 1–7.
- 129. Ciesielski SD, Seed JR, Ortiz JC, Metts J (1992) Intestinal parasites among North Carolina migrant farm workers. Am J Public Health 82: 1258–1262.
- 130. Ortiz JS (1980) The prevalence of intestinal parasites in Puerto Rican farm workers in Western Massachusetts. Am J Public Health 70: 1103–1105.
- 131. Ciesielski S, Seed JR, Estrada J, Wrenn E (1993) The seroprevalence of cysticercosis, malaria, and Trypanosoma cruzi among North Carolina migrant farm workers. Public Health Rep 108: 736–741.
- 132. Watt JP, O'Brien KL, Benin AL, McCoy SI, Donaldson CM, et al. (2007) Risk factors for invasive pneumococcal disease among Navajo adults. Am J Epidemiol 166: 1080–1087.
- 133. Benin AL, Watt JP, O'Brien KL, Reid R, Zell ER, et al. (2005) Delivering pneumococcal vaccine to a high risk population: The Navajo experience. Hum Vaccin 1: 66–69.
- 134. Millar EV, O'Brien KL, Watt JP, Lingappa J, Pallipamu R, et al. (2005) Epidemiology of invasive Haemophilus influenzae type A disease among Navajo and White Mountain Apache children, 1988–2003. Clin Infect Dis 40: 823–830.
- 135. Bockova J, O'Brien KL, Oski J, Croll J, Reid R, et al. (2002) Respiratory syncytial virus infection in Navajo and White Mountain Apache children. Pediatrics 110 (2 Pt 1): e20.
- 136. Rearwin DT, Tang JH, Hughes JW (1997) Causes of blindness among Navajo Indians: An update. J Am Optom Assoc 68: 511–517.
- 137. Ludlam JA (1978) Prevalence of trachoma among Navajo Indian children. Am J Optom Physiol Opt 55: 116–118.
- 138. Pappaioanou M, Schwabe CW, Sard DM (1977) An evolving pattern of human hydatid disease transmission in the United States. Am J Trop Med Hyg 26: 732–742.
- 139. Schantz PM, von Reyn CF, Welty T, Andersen FL, Schultz MG, et al. (1977) Epidemiologic investigation of echinococcosis in American Indians living in Arizona and New Mexico. Am J Trop Med Hyg 26: 121–126.
- 140. Katz R, Murphy S, Kosloske A (1980) Pulmonary echinococcosis: A pediatric disease of the Southwestern United States. Pediatrics 65: 1003–1006.
- 141. Rausch RL (2003) Cystic echinococcosis in the Arctic and Sub-Arctic. Parasitology 127: SupplS73–85.
- 142. Proulx JF, MacLean JD, Gyorkos TW, Leclair D, Richter AK, et al. (2002) Novel prevention program for trichinellosis in Inuit communities. Clin Infect Dis 34: 1508–1514.
- 143. McDonald JC, Gyorkos TW, Alberton B, MacLean JD, Richer G, et al. (1990) An outbreak of toxoplasmosis in pregnant women in Northern Quebec. J Infect Dis 161: 769–774.
- 144. Yoder JS, Beach MJ, Centers for Disease Control and Prevention (CDC) (2007) Giardiasis surveillance–United States, 2003–2005. MMWR Surveill Summ 56: 11–18.
- 145. Kappus KD, Lundgren RG Jr, Juranek DD, Roberts JM, Spencer HC (1994) Intestinal parasitism in the United States: Update on a continuing problem. Am J Trop Med Hyg 50: 705–713.
- 146. Furness BW, Beach MJ, Roberts JM (2000) Giardiasis surveillance–United States, 1992–1997. MMWR CDC Surveill Summ 49: 1–13.
- 147. Yoder JS, Beach MJ, Centers for Disease Control and Prevention (2007) Cryptosporidiosis surveillance–United States, 2003–2005. MMWR Surveill Summ 56: 1–10.
- 148. Truman R, Kearney MT, Lea JW (2005) A summary of Hansen's disease in the United States. US Department of Health and Human Services. Available: http://www.hrsa.gov/hansens/data/summary05.htm. Accessed 10 May 2008.
- 149. Prevention , Centers for Disease and Control (2007) Summary of notifiable diseases - United States. MMWR Morb Mortal Wkly Rep 54: 1–93.
- 150. Putsch RW, Pololi L (2004) Distributive justice in American healthcare: Institutions, power, and the equitable care of patients. Am J Manag Care 10 Spec No: SP45–53.
- 151. Hunt P (2006) The human right to the highest attainable standard of health: New opportunities and challenges. Trans R Soc Trop Med Hyg 100: 603–607.
- 152. Kim K (2006) Time to screen for congenital toxoplasmosis? Clin Infect Dis 42: 1395–1397.
- 153. Garcia HH, Gonzalez AE, Del Brutto OH, Tsang VC, Llanos-Zavalaga F, et al. (2007) Strategies for the elimination of taeniasis/cysticercosis. J Neurol Sci 262: 153–157.
- 154. Nailor MD, Sobel JD (2007) Tinidazole for the treatment of vaginal infections. Expert Opin Investig Drugs 16: 743–751.
- 155. (2004) Tinidazole (Tindamax)–a new anti-protozoal drug. Med Lett Drugs Ther 46: 70–72.
- 156. (2003) Nitazoxanide (Alinia)–a new anti-protozoal agent. Med Lett Drugs Ther 45: 29–31.
- 157. Griffith ME, Hospenthal DR, Murray CK (2006) Antimicrobial therapy of leptospirosis. Curr Opin Infect Dis 19: 533–537.
- 158. Jones JL, Kruszon-Moran D, Won K, Wilson M, Schantz PM (2008) Toxoplasma gondii and Toxocara spp. co-infection. Am J Trop Med Hyg 78: 35–39.
- 159. Yamagata Y, Nakagawa J (2006) Control of Chagas disease. Adv Parasitol 61: 129–165.
- 160. Rocha MO, Teixeira MM, Ribeiro AL (2007) An update on the management of Chagas cardiomyopathy. Expert Rev Anti Infect Ther 5: 727–743.
- 161. Keller TH, Chen YL, Knox JE, Lim SP, Ma NL, et al. (2006) Finding new medicines for flaviviral targets. Novartis Found Symp 277: 102–14. discussion 114–9, 251–3.
- 162. DeVries J (2007) The ABCs of CMV. Adv Neonatal Care 75: 248–55; quiz 256–7.
- 163. (2008) Pediatric Dengue Vaccine Initiative. International Vaccine Institute. Available: http://www.pdvi.org/. Accessed 4 February 2008.
- 164. Schleiss MR, Heineman TC (2005) Progress toward an elusive goal: current status of cytomegalovirus vaccines. Expert Rev Vaccines 4: 381–406.
- 165. U.S. Department of Health and Human Services, National Institutes of Health. National institute of Allergy and Infectious Diseases (2008) FY 2006 fact book. Available: http://www3.niaid.nih.gov/. Accessed 4 February 2008.
- 166. Bell S (2005) From practice research to public policy–the Ministerial Summit on Health Research. Ann Pharmacother 39: 1331–1335.