Figure 1.
Gel electrophoresis of the stool PCR for water buffaloes targeting the Schistosoma japonicum NAD 1 gene.
M, marker. Lane 1, negative control (stool DNA from non-endemic cattle). Lane 2, positive control (S. japonicum adult DNA template). Lane 3–6, water buffalo stool samples done in triplicates. Positive control, lanes 3 and 4 show positive results with bands at 82 bp while none is seen on negative control, lanes 5 and 6.
Figure 2.
Difference in OD values among stool PCR negative and positive in ELISA using SEA and the recombinant proteins.
The graph shows that SjTPx-1 and Sj1TR have the highest number of positives among the PCR positive samples. PCR negative samples that are positive for ELISA using the recombinant proteins show OD values minimally higher than the cut-off values. Mean OD values for each set were also shown.
Table 1.
Results tally of the samples for Schistosoma japonicum using serological tests and stool PCR.
Table 2.
Statistical analysis of the ELISA results of SEA and the recombinant proteins.