LAMPhimerus: A novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples
Fig 2
PCR verification, detection limit and specificity using outer primers F3 and B3 for DNA amplification of Amphimerus sp.
(A) Detection limit of PCR F3-B3. Lane Amp, DNA of Amphimerus sp. (10 ng); lanes 10−1–10−9, 10-fold serial dilutions of Amphimerus sp. DNA. (B) Specificity PCR F3-B3. Lanes Amp, Cs, Ovi, Sm, Sh, Sj, Si, Fh, Dd, Ov, Sv, Ts, Tt, Eg, Cp, Gd, Eh: DNA samples of Amphimerus sp., Clonorchis sinensis, Opisthorchis viverrini, Schistosoma mansoni, S. haematobium, S. japonicum, S. intercalatum, Fasciola hepatica, Dicrocoelium dendriticum, Onchocerca volvulus, Strongyloides venezuelensis, Trichinella spiralis, Taenia truncata, Echinococcus granulosus, Cryptosporidium parvum, Giardia duodenalis and Entamoeba histolytica, respectively. In all panels: lane M, molecular weight marker (100 bp Plus Blue DNA Ladder) and lane N, negative controls (ultrapure water, no DNA template).