Catalase protects Aedes aegypti from oxidative stress and increases midgut infection prevalence of Dengue but not Zika
Fig 3
Catalase knockdown affected both resistance to H2O2 and oviposition.
Catalase was silenced as described, and two days after dsRNA injection, SF (A) or BF (immediately after feeding) (B) mosquitoes were transferred to cages containing 5% sucrose supplemented with 1 M H2O2 ad libitum (day 0). A fresh H2O2 solution was provided daily. Survival was scored every 24 h. *** p < 0.0001 for the comparison between dsLacZ–H2O2 vs dsCat–H2O2 (log-rank test). (C) Catalase-silenced mosquitoes were blood-fed and allowed to lay eggs. Each dot represents an individual mosquito. LacZ–n = 21. Catalase–n = 25. * p = 0.37 (t-test).