Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
Fig 6
Amastigote nests detection by fluorescence microscopy of nasal cavity from mice infected with Dm28c-GFP.
Male BALB/c mice were infected with 1x106 trypomastigotes forms of T. cruzi expressing GFP reporter gene (Dm28c-GFP). At 6 dpi, the nasal cavity tissues were removed, frozen and sections were prepared for fluorescence microscopy analysis. (A) Representative fluorescence image of the nasal cavity from uninfected mice. (B) Representative fluorescence image of the nasal cavity from infected mice. Several amastigote nests (green) can be observed in the nasal cavity. Data represent analysis from an experiment with n = 2.