Plasmodium vivax VIR Proteins Are Targets of Naturally-Acquired Antibody and T Cell Immune Responses to Malaria in Pregnant Women
Five VIR proteins were expressed using the cell-free wheat germ system. Soluble extracts of each were analyzed by western blot using an anti-GST antibody. Purified soluble extracts were analyzed by SDS-PAGE and stained with Blue Coomassie Blue. Molecular weights (MW) markers in kilo-Daltons are shown in the third column, and soluble VIR-GST for the five fusion proteins of predicted sizes are marked with an arrow in columns 1,2,4,5 and 6. GST alone also expressed as a control, is shown in column 7.