Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis
Fig 5
Fungal burden as assessed by CFU from mice lungs treated with AmB, MFLB-AmB, and MFLB.
Lung fragments were weighed, macerated and homogenized in 1 mL cold, sterile PBS. Part of homogenized pulmonary tissue was plated on Petri dishes containing BHI (Brain Heart Infusion) medium, supplemented with 4% (v/v) horse serum, 5% (v/v) supernatant isolated from the infiltrated culture Pb192, and 40 mg/L gentamicin. CFU per gram of lung were determined seven days after plating with incubation at 37°C. Negative Control Group = animals uninfected; Positive Control, MFLB, AmB and MFLB-AmB Groups = animals infected with Paracoccidioides brasiliensis; (AmB = Amphotericin B; MFLB-AmB = nanosized magnetite surface-functionalized with a bilayer of lauric acid conjugated with AmB; MFLB = nanosized magnetite surface-functionalized with a bilayer of lauric acid).