Validation of N-myristoyltransferase as Potential Chemotherapeutic Target in Mammal-Dwelling Stages of Trypanosoma cruzi
Fig 5
DDD compounds inhibit intracellular proliferation of T. cruzi.
(A) Representative images of cells treated with the vehicle control (DMSO), untreated, treated with 800 μM benznidazole (BZ) or 10 μM compound 8, stained with Draq5 (left panel), and analyzed by HCI. Artificial images created after segmentation on the fluorescence bioimager (right panel). Host cells are shown in blue, extracellular parasites in red and intracellular parasites in pink. (B) The multiparametric data obtained on a cell-by-cell basis by HCI was analyzed to determine several parameters associated to infection of host cells by T. cruzi including the percentage of cells infected with at least five parasites (percentage of cells in which the parasite proliferated), treated or not with DDD compounds 1–8. C, controls: 40, 400, and 800 μM BZ, DMSO, and Untreated.