Allicin Induces Calcium and Mitochondrial Dysregulation Causing Necrotic Death in Leishmania
Fig 8
Time-course effect of allicin on ROS levels, intracellular Ca2+, mitochondrial transmembrane potential and ATP production.
Leishmania promastigotes were exposed to allicin (30 μM and 60 μM). Intracellular Ca2+ levels (A), transmembrane mitochondrial potential (B), ATP production (C), cytosolic ROS (D) and mitochondrial ROS levels (E) were determined at 0, 5, 15, 30, 45, 60, 120 and 180 minutes post-treatment. Methods employed for each determination were those given in Material and Methods. Results are the mean ± S.D. of three determinations. Asterisks represent statistically significant differences with untreated controls (*: p<0.05; ** p<0.001).