A Four-Point Screening Method for Assessing Molecular Mechanism of Action (MMOA) Identifies Tideglusib as a Time-Dependent Inhibitor of Trypanosoma brucei GSK3β
Tideglusib [100 nM, GW8510 [100 nM] and DMSO were preincubated with 50 nM TbGSK3 for 30 min. After a 100 fold dilution activity was measured and compared to DMSO containing reaction. Data expressed percent inhibition of DMSO activity. Data represent the average of two separate experiments for GW8510 and three for tideglusib. All points in each experiment were run in duplicate.