Monitoring of the Parasite Load in the Digestive Tract of Rhodnius prolixus by Combined qPCR Analysis and Imaging Techniques Provides New Insights into the Trypanosome Life Cycle
Fig 1
Representative standard qPCR calibration curves generated from 10-fold serial DNA dilutions of blood spiked with T. cruzi Dm28c (A) or CL Brener (B).
The results are expressed as parasite equivalents/50 ng DNA. The slope and regression coefficient of the curves as well as the amplification efficiency for each parasite lineage are indicated.