Carbohydrate Recognition Specificity of Trans-sialidase Lectin Domain from Trypanosoma congolense
Size exclusion chromatography on Superdex200 column and detection at E280nm was used, MWs of different peaks were determined and assigned to oligomeric (1), dimeric (2) and monomeric (3) TconTS1 as described under Methods. A: Oligomerisation pattern of TconTS1 (solid line) or TconTS2 (dashed line), 300 μg protein was loaded. B: Effect of TconTS1 deglycosylation on enzyme oligomerisation. 100 μg TconTS1 were deglycosylated using 4000 units EndoHf glycosidase in phosphate buffer pH 7.4 for 4 hours and directly applied to the column (dashed line). As control 100 μg TconTS1 was treated correspondingly without the addition of enzyme was loaded (solid line).