Carbohydrate Recognition Specificity of Trans-sialidase Lectin Domain from Trypanosoma congolense
A: TconTS2-LD concentration dependent binding to immobilised huS2-Fc (5μg/mL). B: Competitive inhibition of TconTS2-LD binding to huS2-Fc in the presents of serially diluted high-mannose N-glycans. Undiluted inhibitor solution was set to 1.0. The maximum increase in relative fluorescence units (RFU) over time was determined as described under Methods. Data points are means ± standard deviation of triplicates.