Improved Quantification, Propagation, Purification and Storage of the Obligate Intracellular Human Pathogen Orientia tsutsugamushi
Fig 7
Optimising freezing conditions for preserving bacterial viability.
Panel A. Comparing the growth of O. tsutsugamushi after being frozen in lysed or intact host cells, using SPG media. Panel B. Growth of bacteria after freezing in intact host cells in different buffers. Panel C. A comparison of different freezing buffers on the subsequent growth of purified bacteria and Panel D. Growth of O. tsutsugamushi after being frozen in SPG media at different freezing and thawing speeds. FF = fast freeze, SF = slow freeze, FT = fast thaw and ST = slow thaw. Panel E. Growth of O. tsutsugamushi after being frozen and stored for 40 mins or 1 week at-80°C. Bacteria were purified and stored in SPG buffer.