Pentoxifylline Reverses Chronic Experimental Chagasic Cardiomyopathy in Association with Repositioning of Abnormal CD8+ T-Cell Response
Fig 2
PTX influenced the balance of inflammatory and cytotoxic profiles systemically and in the heart tissue.
(A) ELISpot assay identifying the functional capacity of IFNγ-producing CD8+ T-cells recognizing T. cruzi crude extracts and the H-2Kb-restricted ASP2 (VNHRFTLV) immunodominant peptide. (B) Frequencies of IFNγ+, IFNγ+CD107a+ and CD107a+ CD8+T-cells in spleen. (C) Frequencies of IFNγ+, IFNγ+PFn+ and PFn+ CD8+T-cells in spleen. Dotted lines show mean frequencies in noninfected (NI) controls. (D) Immunohistochemistry for detection of IFNγ+ and Pfn+ cells in the heart tissue. The results represent three to five mice per experimental group in three independent experiments. * p<0.05, ** p<0.01 and *** p<0.001, saline-injected T. cruzi-infected mice compared with NI controls. # p<0.05 and ## p<0.01, saline-injected compared with PTX-treated T. cruzi-infected mice.