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Live Imaging of Bioluminescent Leptospira interrogans in Mice Reveals Renal Colonization as a Stealth Escape from the Blood Defenses and Antibiotics

Figure 5

Effect of antibiotic treatments at the chronic phase of leptospirosis.

C57BL/6J mice chronically infected for 25 days (D25) with 107 MFlum1 were (A) IP injected daily for 7 days with penicillin G (Pen) (brown) or Ciprofloxacin (Cipr) (blue) from 25 until 31 dpi (T1 to T7), (B) injected daily for 7 days with azithromycin (Azi) from 25 until 31 dpi, and from 112 until 118 dpi (T1 to T7). The arrows indicate the duration of the different treatments. All the bioluminescence analyses were done after IP administration of D-luciferin. Data are expressed as the mean ± SEM of average radiance of light measured in photons/second/cm2 in n = 4 infected treated or untreated mice. p values (+p<0.05, ++p<0.01, +++p<0.001) are indicated in corresponding colors for each group versus the uninfected group. Below are shown images of the tracking of one untreated, infected (MFlum1) mouse and an infected treated (Azi) mouse photographed at different crucial time points. The corresponding p values (*p<0.05) were calculated between infected untreated group and treated group (Azi). Images depict photographs overlaid with color representations of luminescence intensity, measured in photons/second/cm2 and indicated on the scales, where red is most intense and purple is least intense. PT, days post treatment.

Figure 5