Cytokine Production but Lack of Proliferation in Peripheral Blood Mononuclear Cells from Chronic Chagas' Disease Cardiomyopathy Patients in Response to T. cruzi Ribosomal P Proteins
Figure 5
Cytokine production profile by CD4+ and CD8+ T cells derived from chagasic patients.
CD4+ and CD8+ T cells from three chronic Chagas' disease Cardiomyopathy patients, called RM11, RM12 and RM14, were isolated as described under Methods. Enriched CD4+ or CD8+ T cells were co-cultured with the autologous CD3(neg) fraction as antigen presenting cells and the indicated stimulus. Supernatants were collected on day 6 and cytokines quantified by multiplex technology. For comparison, results from whole PBMC from each of the patients are represented in the left panel. Symbol (+) indicates a fold induction between 3 to 5 and (++) a fold induction>5. The fold induction was calculated as: [(cytokine in stimulated culture) - (cytokine in NS culture)]/(cytokine in NS culture), where NS denotes non-stimulated cells.