Fitness Impact and Stability of a Transgene Conferring Resistance to Dengue-2 Virus following Introgression into a Genetically Diverse Aedes aegypti Strain
Figure 10
Comparison of observed transgenic allele frequencies over five generations (F1–F5) of Carb109F/GDLS.BC5 and Carb109M/GDLS.BC5.
Three replicate crosses (F.1, F.2, F.3) between Carb109F/GDLS.BC5 heterozygotes (200 individuals in each cross) and three replicate crosses (M.1, M.2, M.3) between Carb109M/GDLS.BC5 heterozygotes (200 individuals/cross) were performed in separate cages. All resulting wild-type larvae were culled. Remaining transgenic individuals were allowed to inter-mate. This procedure was followed for five generations (F1–F5). Results were compared to values expected under Fisher's Selection Model. (A) Observed frequencies of EGFP- expressing Carb109F/GDLS.BC5 larvae of replicates F.1, F.2, F.3 compared to values predicted under Fisher's Selection model (redline). (B) Observed frequencies of EGFP-expressing Carb109M/GDLS.BC5 larvae of replicates M.1, M.2, M.3 in comparison to values predicted under Fisher's Selection model (redline). Proportions were compared by estimating the 95% HDI (error bars) with WinBUGS and the Credible Intervals for Proportions script [44].