Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex
Figure 6
Comparison of electrophoretic profile (A) and Bothrops antivenom antigenic reactivity (B) of venoms from snakes classified in different genera.
Samples containing 10 µg Bothropoides jararaca (JAR), Bothropoides neuwiedi (NEU), Bothrops atrox (ATR), Bothrops jararacussu(JSU), Rhinocerophis alternatus (ALT) and Rhinocerophis cotiara (COT) venoms were fractionated by SDS-PAGE (12.5% acrylamide gels) under non-reducing conditions and were either stained with Coomassie blue (A) or transferred to nitrocellulose membranes, which were then incubated with SAB (1∶1,000) as the primary antibody and peroxidase-labeled goat anti-horse IgG (1∶1,000). The reactive bands were detected by incubation with 4-chloro-α-naphthol and H2O2 (B). The numbers at the left indicate the mobility of the molecular mass markers in kDa. These results represent three independent runs.