In Vitro Silencing of Brugia malayi Trehalose-6-Phosphate Phosphatase Impairs Embryogenesis and In Vivo Development of Infective Larvae in Jirds
Figure 5
Soaking B. malayi adult female worms in siRNA results in Bm-tpp gene-specific inhibition of expression.
Quantative Real time PCR. Two female worms at different time interval (12, 24, 36, 48, 60 h) and 2 day after treatment were removed and analyzed for difference in tpp and gpd gene-specific transcript levels by qRT-PCR. The relative amounts of tpp and gpd amplicon were determined by using the comparative ΔCT method and normalizing against the endogenous control gene (Bm-tub-1). The median value of the control group was set to 100% and the percent relative mRNA level was shown. (A) Soaking B. malayi adult female worms in siRNA results in tpp gene-specific inhibition of expression. (B) siRNA treatment of adult worms non significantly effects the expression level of gpd mRNA. The criterion for statistical significance between the results was p value<0.05 was considered significant marked as *, <0.01 as highly significant**, <0.001 *** as very highly significant.