Whole Organism High-Content Screening by Label-Free, Image-Based Bayesian Classification for Parasitic Diseases
(A) Combined phenotype and motility analysis of wells treated with 10 µM of the compounds. Visually assessed hits are shown in red, non-hits in blue and non-hits with compound crystals in the well in yellow. The hit region for automated image analysis as determined above using the anti-schistosome compounds is delineated by the dashed lines. (B) Performance of the Automated image analysis categorization compared to the visual assessments. (C) Z factor analysis of phenotype (open) and motility (closed) per plate of the Dundee compound library.