Female-Specific Flightless (fsRIDL) Phenotype for Control of Aedes albopictus
A: Map of the OX4358 construct. Promoters are indicated by arrows, exons are shown as boxes, introns as horizontal lines. The engineered start codon is indicated by a bar in the Actin-4 exon 1 (Ex1), whilst stop codons in the male exon are shown by bars below the line. B: Phenotype of wild-type (left) and OX4358F1-Aal (right) pupae under white light (left panel) and blue filter (right panel). C: RT-PCR analysis of transcripts from Ae. albopictus OX4358, from two male and two female pupae (M1, M2, F1 and F2, respectively), gDNA amplification (g) and no-template control (c). L: DNA size marker (Smartladder, Eurogentec). D: RT-PCR analysis of transcripts from Ae. aegypti OX4358, from three male and three female pupae (M1, M2, M3, F1, F2, and F3 respectively) and no template control (c). Sequencing of the PCR products revealed that splicing occurs as in the native gene, except for a second male-specific transcript in which exon 1 has an extra 75 bp in both Ae. aegypti and Ae. albopictus (see text).