Female-Specific Flightless (fsRIDL) Phenotype for Control of Aedes albopictus
A: Gene structure of Aedes albopictus and Aedes aegypti Actin-4. Putative promoters are indicated by arrows, exons are shown as boxes, introns as lines. Non-coding 5′ and 3′UTR are shaded pale grey; the male-specific exons are shaded dark grey. B: RT-PCR confirming differential splicing in male (M) and female (F) Ae. albopictus pupae; genomic (g) and no template control (c) are also shown. L: DNA size marker (Smartladder, Eurogentec). Sizes of the major bands are consistent with the predicted gene structure (panel A and Table S1); sequence of the cDNA bands confirmed splice sites (not shown).