Integrated Dataset of Screening Hits against Multiple Neglected Disease Pathogens
Figure 2
Screening logistics and framework.
The formatting and distribution of the library to various screening centers on dry ice was handled by 2 screening partners - Laboratory for Microbiology, Parasitology and Hygiene at the University of Antwerp (LMPH) and the London School of hygiene and Tropical Medicine (LSHTM). Two shipments were lost (indicated by dark grey boxes) due to logistics problems. Considering the high variability of screening throughput (P. falciparum = 100,000 compounds/month, T. cruzi, T. brucei & L. infantum = 10,000 compounds/month, while S. mansoni (adult worms), B. malayi (adult worms and microfilaria) and O. lienalis/O. ochengi (microfilaria) = 1,000 compounds/month), shipments were handled in 3 batches from increasing size. For batch 2 and 3 and for diseases having 2 different screening sites (Schistosomiasis with London School of Hygiene and Tropical Medicine (LSHTM) and Theodor Bilharz Institute (TBRI) in Cairo or Onchocerciasis with Northwick Park Institute for Medical Research (NPIMR) using O. lienalis and the University of Buea (UB) using O. ochengi) it was decided to have molecules individually tested on one single site.