An Atlas for Schistosoma mansoni Organs and Life-Cycle Stages Using Cell Type-Specific Markers and Confocal Microscopy
Figure 6
Labeling of non-reproductive tissues in adult S. mansoni.
(A) Lectin PNA staining of the esophageal gland cells. Lectin sWGA labeling of cephalic ganglia and protonephridial ducts in a female. Image is a maximum intensity projection. (B) Lectin sWGA labeling of cephalic ganglia and non-ciliated protonephridial ducts of a male. Also shown is anti-acetylated α-tubulin staining the ciliated regions of the protonephridial system. Image is a maximum intensity projection. (C) Maximum intensity projection depicting anti-acetylated α-tubulin staining of the ciliated regions of the male protonephridial system, including the flame cells and the collecting ducts. Note that many secondary and tertiary ciliated ducts funnel into one of the two main collecting ducts that run along the longitudinal axis. (D) Maximum intensity projection of a protonephridial unit labeled with anti-acetylated α-tubulin and lectin sWGA. Anti-acetylated α-tubulin marks the ciliated flame cells and ducts, whereas sWGA labels non-ciliated tubules. Scale bars, 10 µm. Anterior faces to the upper left in panels A and B.