Towards Delineating Functions within the Fasciola Secreted Cathepsin L Protease Family by Integrating In Vivo Based Sub-Proteomics and Phylogenetics
Figure 5
Novel CL1D protease sequences.
Boxed and shaded in light grey are the predicted signal peptides using SignalP 3.0. Boxed and shaded in dark grey is the conserved GXNXFXD motif for autoactivation. Arrowed are the five amino acid substitutions varying between both contigs with the associated nucleotide substitutions above each. Individual boxed amino acids correspond to the active site residues with the exception of one, labelled with a *. This corresponds to the leucine at position 69 (papain numbering) dictating substrate specificity [61], [62]. The dashed line indicates the start of the N-terminus of the mature enzyme. A primary consensus sequence (Prim.cons.) is also included. Alignment was performed using ClustalW [63].