RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening
Figure 5
RNAi of more than one gut-associated gene transcript is robust and selective.
Newly transformed schistosomula were incubated for 6 days with either 30 or 60 µg/ml of the indicated dsRNA preparations depending on whether single or double transcript suppression of SmCB1 (A) or SmCD (B) was being attempted. RNAi was measured by qRT-PCR and data expressed relative to those following parasite exposure to schistosome-unspecific mCherry dsRNA at 60 µg/ml. Included as controls were parasites that had not been exposed to dsRNA (−ve) or that had been co-incubated with dsRNA to the cysteine protease inhibitor cystatin (Cys). Each sample was tested in duplicate and representative data from two experiments are shown.