Vaccination with DNA Plasmids Expressing Gn Coupled to C3d or Alphavirus Replicons Expressing Gn Protects Mice against Rift Valley Fever Virus
Figure 6
Weight loss curves and survival against virulent RVFV challenge of mice vaccinated with indicated vaccine regimens.
Mice vaccinated with indicated vaccines or appropriate controls, DNA plasmid with no insert (DNA control) and replicon expressing influenza HA (replicon control) were challenged with 1000 PFU of RVFV ZH501 and monitored for loss in body weight (A) to (C) and mortality (D) daily post-challenge. Dead and moribund mice were included in the weight loss curves on the day of death, but not after. The daily weight of each mouse was compared to her weight on the day of challenge, and data are shown as the average percentage of initial weight for each cohort. Error bars represent the standard error for all samples available at that time point. A two-way ANOVA with Bonferroni's post tests was used to determine the significance of the body weight data between groups, which is denoted by asterisks; *P<0.05, **P<0.01, *** P<0.001. All vaccinated mice showed statistically significant protection (P<0.05, log rank test) compared to unvaccinated mice.