A summary report from a Global HIV Vaccine Enterprise Working Group
Citation: Montefiori D, Sattentau Q, Flores J, Esparza J, Mascola J, on behalf of a Working Group convened by the Global HIV Vaccine Enterprise (2007) Antibody-Based HIV-1 Vaccines: Recent Developments and Future Directions. PLoS Med 4(12): e348. doi:10.1371/journal.pmed.0040348
Published: December 1, 2007
Copyright: © 2007 Montefiori et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Participation in the workshop was funded by the Global HIV Vaccine Enterprise. The authors received no additional funding for this article.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: FcR, Fc receptor; MAb, monoclonal antibody; PBMC, peripheral blood mononuclear cells; SHIV, chimeric simian-human immunodeficiency virus; SIV, simian immunodeficiency virus
The Global HIV Vaccine Enterprise convened a two-day workshop in May of 2007 to discuss humoral immune responses to HIV and approaches to design vaccines that induce viral neutralizing and other potentially protective antibody responses. The goals of this workshop were to identify key scientific issues, gaps, and opportunities that have emerged since the Enterprise Strategic Plan was first published in 2005 , and to make recommendations that Enterprise stakeholders can use to plan new activities.
Most effective viral vaccines work, at least in part, by generating antibodies that inactivate or neutralize the invading virus, and the existing data strongly suggest that an optimally effective HIV-1 vaccine should elicit potent antiviral neutralizing antibodies. However, unlike acute viral pathogens, HIV-1 chronically replicates in the host and evades the antibody response. This immune evasion, along with the large genetic variation among HIV-1 strains worldwide, has posed major obstacles to vaccine development. Current HIV vaccine candidates do not elicit neutralizing antibodies against most circulating virus strains, and thus the induction of a protective antibody response remains a major priority for HIV-1 vaccine development. For an antibody-based HIV-1 vaccine, progress in vaccine design is generally gauged by in vitro assays that measure the ability of vaccine-induced antibodies to neutralize a broad spectrum of viral isolates representing the major genetic subtypes (clades) of HIV-1 . Although it is not known what magnitude and breadth of neutralization will predict protection in vaccine recipients, it is clear that current vaccine immunogens elicit antibodies that neutralize only a minority of circulating isolates. Thus, much progress needs to be made in this area. Also, though virus neutralization is considered a critical benchmark for a vaccine, this may not be the only benchmark for predicting success with antibody-based HIV-1 vaccine immunogens.
The main targets for neutralizing antibodies to HIV-1 are the surface gp120 and trans-membrane gp41 envelope glycoproteins (Env) that mediate receptor and coreceptor binding and the subsequent membrane fusion events that allow the virus to gain entry into cells . Antibodies neutralize the virus by binding these viral spikes and blocking virus entry into susceptible cells, such as CD4+ T cells [4,5]. In order to chronically replicate in the host, the virus exploits several mechanisms to shield itself against antibody recognition, including a dense outer coating of sugar molecules (N-linked glycans) and the strategic positioning of cysteine–cysteine loop structures on the gp120 molecule [6–8]. These shielding mechanisms, although highly effective, have vulnerabilities imposed by fitness constraints. Information on the precise location and molecular structure of these vulnerable regions could be valuable for the rational design of improved vaccine immunogens.
Participants in the workshop identified four areas that, if given proper attention, could provide key information that would bring the field closer to an effective antibody-based HIV-1 vaccine: (1) structure-assisted immunogen design, (2) role of Fc receptors and complement, (3) assay standardization and validation, and (4) immunoregulation of B cell responses.
Structure-Assisted Immunogen Design
Clinical studies have demonstrated that immunization with the gp120 surface unit of the HIV-1 envelope protein does not lead to the induction of potent or broadly reactive neutralizing antibodies. In order to develop better immunogens, it is likely that we will need a more detailed understanding of the atomic level structure of epitopes on the native envelope glycoprotein. Data on the X-ray crystal structure of liganded and unliganded partial gp120 molecules have provided valuable information about the atomic level interaction of gp120 and neutralizing antibodies [9–12]. The recent atomic level resolution of monoclonal antibody (MAb) b12 bound to the CD4 receptor binding site of the gp120 molecule provides new insights into how successful neutralizing antibodies access functionally conserved regions of the Env glycoprotein . Crystal structures of complete monomeric gp120 and gp120–gp41 trimer complexes in their native unliganded form need to be elucidated, as these are the natural targets for neutralizing antibodies. This information is needed for multiple genetic subtypes of the virus and for transmitted strains of the virus. Coupled with this effort should be a program to make necessary improvements in electron tomography technology to gain a higher resolution of native Env spikes as they exist on virus particles [14–16]. An improved understanding of the structural basis of antibody binding to the HIV-1 Env glycoprotein will likely form the foundation for a rational program of novel vaccine design. Ongoing efforts to stabilize gp120 into more immunogenic forms or to scaffold conserved neutralization epitopes into foreign proteins may lead to more promising antibody responses.
Induction of an effective neutralizing antibody response will require that a vaccine deliver to the naïve B cell repertoire epitopes that are both immunogenic (i.e., possess favorable properties for B cell inductive pathways) and antigenic (i.e., available for high affinity antibody binding on functional Env spikes). Viral epitopes that are conserved among most viral strains are more likely to generate cross-reactive antibodies. In this regard, researchers have focused on a small number of human MAbs, from clade B HIV-1-infected individuals, that possess broadly cross-reactive neutralizing activity [17,18]. The cognate viral epitopes for these MAbs have been well characterized and are being evaluated as vaccine immunogens. However, for reasons that are not completely understood, these conserved viral epitopes have either been poorly immunogenic or have elicited antibodies of restricted reactivity. Improvements are being sought by introducing specific structural alterations [19,20] and by targeting autoreactive B cell pathways . These and other efforts to improve the immunogenicity of conserved neutralization epitopes should remain a high priority. Workshop participants recognized the need to expand efforts to identify and characterize new MAbs, with special attention to MAbs from non-clade B HIV-1 infections. New technologies are now available that might afford an advantage for identifying novel antibody specificities that were previously undetected [22,23]. In addition to this focus on MAbs, sera from selected HIV-1-infected individuals that can broadly neutralize HIV-1 isolates should be studied in detail. New assays allow more precise mapping of the polyclonal antibody response in these sera to better understand the epitopes targeted [5,24–26]. Such studies may reveal novel antibody specificities and their associated viral epitopes that could be useful for immunogen design.
While there has been considerable interest in conserved epitopes, less attention has been paid to more variable epitopes that might be useful if administered in the form of a polyvalent vaccine. Of particular interest are the epitopes that drive the autologous neutralizing antibody response in infected individuals. These epitopes may be quite variable, but recent evidence suggests that there are constraints on the extent of variation the virus can tolerate in these regions [27,28]. Detailed molecular and immunologic studies of the autologous neutralization response would enhance our understanding of viral determinants that are vulnerable to antibody attack. Similarly, it is possible that combinations of antibodies will have desirable additive or synergistic effects on virus neutralization [29–32]. An example is seen in how soluble CD4 binding rearranges the structure of gp120 to expose the highly conserved coreceptor binding domain, which allows antibody binding and virus neutralization to occur [33,34]. Such effects of antibodies might be discovered by applying high throughput screening methods to the plethora of existing MAbs as well as new MAbs that become available in the future.
Role of Fc Receptors and Complement
Recent findings have generated renewed interest in so-called “non-neutralizing” antibodies that are unable to directly inhibit free virus entry into target cells, but nonetheless exhibit antiviral activity mediated by the Fc region of the antibody molecule. These antibody effector mechanisms include complement binding and viral lysis, phagocytosis of antibody-coated virions, and antibody-dependent cellular cytotoxicity [35–38]. Recent studies have suggested examples of Fc-dependent antiviral effects of HIV-1-positive serum in cases where there was little or no detectable activity in conventional neutralization assays [39,40].
In addition, passive transfer studies in a relevant monkey model suggest that Fc receptor (FcR) binding capacity of a protective antibody makes a substantial contribution to the antibody-mediated protection . Antibody effector functions that mediate complement activation and FcR engagement on macrophages, dendritic cells, natural killer cells, and other cell types need to be evaluated to determine their relevance to HIV-1 vaccines. Assays that measure these antiviral antibodies should be standardized and used to assess biologic relevance in passive protection experiments in animal models using antibodies that exhibit the different effector functions in vitro.
Assay Standardization and Validation
In order to adequately monitor neutralization breadth and potency and to compare and prioritize immunogens, assays are needed that are sensitive, quantitative, high throughput, and have correlative value. Substantial improvements have been made in the past several years in assay technology and in available reference reagents. Thus, cumbersome and expensive assays using peripheral blood mononuclear cells (PBMC) and uncloned viruses are being replaced with assays that utilize molecularly cloned Envpseudotyped viruses and genetically engineered target cells lines [2,42–45]. This new technology affords greater sensitivity, reproducibility, high throughput, and cost-effectiveness compared to PBMC assays, and as a result, it has been responsible for an explosion of new data. Steps are being taken by the Collaboration for AIDS Vaccine Discovery to transfer this new technology to multiple laboratories around the world and to implement a validated proficiency testing program to assure inter-laboratory equivalency in assay performance.
Recently, several cases were identified where neutralization was considerably more potent or only detected in the older PBMC assay compared to the newer assay technology [43,46,47]. This raises important questions about current plans to employ a single assay for routine use, and it points to the need for a better understanding of the mechanisms of neutralization. Thus, it may be necessary to use more than one assay to assure that all neutralizing antibodies are detected. There is a need to standardize and compare neutralizing antibody assays and to decide which assay or combination of assays should be used for standardized assessments of vaccine-elicited neutralizing antibody responses. A major priority is to strengthen the standardization of the PBMC assay, given that it is the only assay that has been at least partially validated in passive antibody experiments in animal challenge models.
Important decisions need to be made about the types of antibodies and assays that have greatest relevance to HIV-1 vaccines. Validation experiments in animals models are needed to determine the potential correlative value of new assay technologies that rely on the use of genetically engineered cells lines and Envpseudotyped viruses. Ideally, this would be done by employing several different assays to study the antibody response in a clinical efficacy trial in which the vaccine was at least partially protective. Because no such vaccine is currently available for HIV-1, studies in animal models are the next best choice. In this regard, two animal models are widely used for HIV vaccine development: simian immunodeficiency virus (SIV) and chimeric simian-human immunodeficiency virus (SHIV) infection in monkeys . Quantitative passive transfer experiments in either model with antibodies that exhibit different effector functions could be used to address the biological relevance of in vitro assays. Unfortunately, very few SHIVs are currently available and, among these, most are derived from a single genetic subtype (clade B) and exhibit properties that may not be well suited to assay validation . The creation of new and better SHIVs from non-clade B viruses would facilitate assay standardization as well as vaccine challenge models.
Immunoregulation of B Cell Responses
This workshop identified several critical gaps in the current understanding of B cell regulatory pathways that impede a more rational development of an effective antibody-based HIV-1 vaccine. For example, broadly neutralizing antibodies in patient serum bind epitopes that are present on monomeric gp120 , yet this is a poor immunogen for neutralizing antibody induction in vaccine recipients. Moreover, as mentioned above, viral epitopes for the known broadly neutralizing MAbs appear to be poorly immunogenic in infected individuals and as vaccine candidates. Insights into the immunoregulation of some of these latter epitopes (e.g., epitopes defined by MAbs 2F5 and 4E10) was provided by recent studies in which the MAbs were discovered to bind one or more self antigens [50,51], raising the possibility that these antibody specificities are subjected to negative regulation mechanisms, such as receptor editing or deletion. Thus, Env as an immunogen may bypass key steps in the B cell inductive pathway, or may actively induce negative production or downregulation of production of some broadly neutralizing antibodies [52–54].
The receptor–ligand interactions and intracellular signaling pathways that govern the production of antibody-producing plasma cells and the persistence of plasma and memory B cells are poorly understood. Additional information on the mechanisms responsible for B cell migration, selection, and differentiation within and between specialized anatomical sites, particularly within lymphoid follicles, might be used to target suitable Env epitopes to appropriate B cell inductive pathways. An example would be to provide necessary signals to generate long-lived and high affinity memory in the marginal zone B cell compartment. Another example would be to discover ways to modify germinal center formation, positive and negative selection, and B cell differentiation to drive long-lived high affinity antibody responses against key epitopes that tend to be poorly immunogenic.
In parallel to these efforts, genetic studies at the population level could provide critical information on the most promising paths to follow. In particular, the recent completion of the International HapMap Project now permits whole genome associated studies to be conducted with a minimum number of single nucleotide polymorphism tags [55,56]. This powerful new technology could be used to identify genes that are associated with the wide variation in neutralizing antibody responses in HIV-1-infected individuals and in vaccine recipients. A critical question to ask is whether the potent neutralizing antibody response in a small subset of infected individuals is due to unique viral epitopes or to host genetic polymorphisms. Current evidence suggests that both might make a substantial contribution in the context of combined epitope and allelic representations [28,47,57].
To date most studies of the humoral responses in HIV infections have investigated immunoglobulins, the final product of B cell responses. Relatively few studies have examined B cell immunopathogenesis. A number of basic questions are still unanswered (e.g., extent and reason for perturbation of B cell subset changes, including memory B cells and plasma cells, in peripheral blood and tissues). Questions also remain about other potential functional contributions of B cells to HIV infections (e.g., role as antigen-presenting cells). In vivo studies should be performed in the nonhuman primate animal model to determine the emergence of pathologic events in the B cell compartment, in particular in lymphatic and gastrointestinal tissues of naïve and vaccinated animals that are challenged with pathogenic SIV or SHIV. These investigations should be done in parallel to detailed analyses of the magnitude and function of HIV-specific immunoglobulin responses determined in plasma and tissue secretions, and of HIV-specific B cells on a single cell basis.
The establishment of a research consortium to study fundamental B cell biology as it relates to HIV-1 vaccines is recommended. This program should be structured in a way that asks key scientific questions about B cell regulatory pathways that modulate Env immunogenicity. Studies could address B cell receptor–ligand interactions and intracellular signaling pathways that govern the production of antibody-producing plasma cells, the persistence of plasma and memory B cells, the mechanism of action of adjuvants, and host genetic associations with immune responses.
DM, QS, and JM organized the Enterprise workshop with the collaboration of JF and JE, and all of them contributed to writing the paper. Participants in the workshop contributed with formal presentations, discussions, and editing of the paper. The other members of the Enterprise Working Group were: James Bradac, Donald S. Burke, Emily Carrow, Robert Carter, Andrea Cerutti, Raphaelle El Habib, Donald N. Forthal, Barton F. Haynes, Gunilla Karlsson Hedestam, Peter Kwong, Christiane Moog, Victoria R. Polonis, Helen Quill, Gabriella Scarlatti, Jörn Schmitz, George M. Shaw, Sriram Subramaniam, Gerald Voss, Drew Weisman, and Richard Wyatt.
- 1. Coordinating Committee of the Global HIV/AIDS Vaccine Enterprise (2005) The Global HIV/AIDS Vaccine Enterprise: Scientific strategic plan. PLoS Med 2: e25. doi:10.1371/journal.pmed.0020025.
- 2. Mascola JR, D'Souza P, Gilbert P, Hahn B, Haigwood NL, et al. (2005) Recommendations for the design and use of standard virus panels to assess neutralizing antibody responses elicited by candidate human immunodeficiency virus type 1 vaccines. J Virol 79: 10103–10107.
- 3. Wyatt R, Sodroski J (1998) The HIV-1 envelope glycoproteins: Fusogens, antigens, and immunogens. Science 280: 1884–1888.
- 4. Crooks ET, Moore PL, Richman D, Robinson J, Crooks JA, et al. (2005) Characterizing anti-HIV monoclonal antibodies and immune sera by defining the mechanism of neutralization. Hum Antibodies 14: 101–113.
- 5. Moore PL, Crooks ET, Porter L, Porter L, Zhu P, et al. (2006) The nature of non-functional envelope proteins on the surface of human immunodeficiency virus type 1. J Virol 80: 2515–2528.
- 6. Wei X, Decker JM, Wang S, Hui H, Kappes JC, et al. (2003) Antibody neutralization and escape by HIV-1. Nature 422: 307–312.
- 7. Bou-Habib DC, Roderiquez G, Oravecz , T , Berman , PW , Lusso P, et al. (1994) Cryptic nature of envelope V3 region epitopes protects primary monocytotropic human immunodeficiency virus type 1 from antibody neutralization. J Virol 68: 6006–6013.
- 8. Cao J, Sullivan N, Desjardin E, Parolin C, Robinson J, et al. (1997) Replication and neutralization of human immunodeficiency virus type 1 lacking the V1 and V2 variable loops of the gp120 envelope glycoprotein. J Virol 71: 9808–9812.
- 9. Kwong PD, Wyatt R, Robinson J, Sweet RW, Sodroski J, et al. (1998) Structure of an HIVgp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody. Nature 393: 648–659.
- 10. Kwong PD, Wyatt R, Majeed S, Robinson J, Sweet RW, et al. (2000) Structures of HIV-1 gp120 envelope glycoproteins from laboratory-adapted and primary isolates. Struct Fold Des 8: 1329–1339.
- 11. Chen B, Vogan EM, Gong H, Skehel JJ, Wiley DC, et al. (2005) Structure of an unliganded simian immunodeficiency virus gp120 core. Nature 433: 834–841.
- 12. Huang C-C, Tang M, Zhang MY, Majeed S, Montabana E, et al. (2005) Structure of a V3-containing HIV-1 gp120 core. Science 310: 1025–1028.
- 13. Zhou T, Xu L, Dey B, Hessell AJ, Van Ryk D, et al. (2007) Structural definition of a conserved neutralization epitope on HIV-1 gp120. Nature 445: 732–737.
- 14. Zhu P, Liu J, Bess J Jr, Chertova E, Lifson JD, et al. (2006) Distribution and three-dimensional structure of AIDS virus envelope spikes. Nature 441: 847–852.
- 15. Zanetti G, Briggs JAG, Grünewald K, Sattentau QJ, Fuller SD (2006) Cryo-electron tomographic structure of an immunodeficiency virus envelope complex in situ. PLoS Pathog 2: e83. doi:10.1371/journal.ppat.0020083.
- 16. Sougrat R, Bartesaghi A, Lifson JD, Bennett AE, Bess JW, et al. (2007) Electron tomography of the contact between T cells and SIV/HIV–1: Implications for viral entry. PLoS Pathog 3: e63. doi:10.1371/journal.ppat.0030063.
- 17. Pantophlet R, Burton DR (2006) GP120: Target for neutralizing HIV-1 antibodies. Annu Rev Immunol 24: 739–769.
- 18. Haynes BF, Montefiori DC (2006) Aiming to induce broadly reactive neutralizing antibody responses with HIV-1 vaccine candidates. Expert Rev Vaccines 5: 347–363.
- 19. Phogat S, Wyatt R (2007) Rational modifications of HIV-1 envelope glycoproteins for immunogen design. Cur Pharm Design 13: 213–227.
- 20. Burton DR, Desrosiers RC, Doms RW, Koff WC, Kwong PD, et al. (2004) HIV vaccine design and the neutralizing antibody problem. Nat Med 5: 233–236.
- 21. Haynes BF, Moody MA, Verkoczy L, Kelsoe G, Munir SM (2005) Antibody polyspecificity and neutralization of HIV-1: A hypothesis. Hum Antibodies 14: 59–67.
- 22. Bowley DR, Labrijn AF, Zwick MB, Burton DR (2007) Antigen selection from an HIV-1 immune antibody library displayed on yeast yields many novel antibodies compared to selection from the same library displayed on phage. Protein Eng Design Selection 20: 81090.
- 23. Traggiai E, Becker S, Subbarao , K , Kolesnikova L, Uematsu Y, et al. (2004) An efficient method to make human monoclonal antibodies from memory B cells: Potent neutralization of SARS coronavirus. Nat Med 10: 871–875.
- 24. Yuste E, Sanford HB, Carmody J, Bixby J, Little S, et al. (2006) Simian immunodeficiency virus engrafted with human immunodeficiency virus type 1 (HIV-1)-specific epitopes: Replication, neutralization, and survey of HIV-1-positive sera. J Virol 80: 3030–3041.
- 25. Li Y, Migueles S, Welcher B, Svehla K, Phogart A, et al. (2007) Broad HIV-1 neutralization mediated by CD4 binding site antibodies. Nat Med 13: 1032–1034.
- 26. Dhillon AK, Donners H, Pantophlet R, Johnson WE, Decker JM, et al. (2007) Dissecting the neutralizing antibody specificities of broadly neutralizing sera from human immunodeficiency virus type 1-infected donors. J Virol 81: 6548–6562.
- 27. Deeks SG, Schweighardt B, Wrin T, Galovich J, Hoh R, et al. (2006) Neutralizing antibody responses against autologous and heterologous viruses in acute versus chronic human immunodeficiency virus (HIV) infection: Evidence for a constraint on the ability of HIV to completely evade neutralizing antibody responses. J Virol 80: 6155–6164.
- 28. Draenert R, Allen TM, Liu Y, Wrin T, Chappey C, et al. (2006) Constraints on HIV-1 evolution and immunodominance revealed in monozygotic adult twins infected with the same virus. J Exp Med 203: 529–539.
- 29. Montefiori DC, Graham BS, Zhou JT, Zhou JY, Bucco R, et al. (1993) V3-specific neutralizing antibodies in sera from HIV-1 gp160-immunized volunteers block virus fusion and act synergistically with human monoclonal antibody to the conformation-dependent CD4 binding region of gp120. J Clin Invest 92: 840–847.
- 30. Thali M, Furman C, Wahren B, Posner M, Ho DD, et al. (1992) Cooperativity of neutralizing antibodies directed against the V3 and CD4 binding regions of the human immunodeficiency virus gp120 envelope glycoproteins. J Acquired Immune Defic Syndr 5: 591–599.
- 31. Tilley SA, Honnen WJ, Racho ME, Chou T-C, Pinter A (1992) Synergistic neutralization of HIV-1 by human monoclonal antibodies against the V3 loop and the CD4-binding site of gp120. AIDS Res Hum Retroviruses 8: 461–467.
- 32. Li A, Katinger H, Posner MR, Cavacini L, Zolla-Pazner S, et al. (1998) Synergistic neutralization of simian-human immunodeficiency virus SHIV-vpu+ by triple and quaduple combinations of human monoclonal antibodies and high-titer anti-human immunodeficiency virus type 1 immunoglobulins. J Virol 72: 3235–3240.
- 33. Sullivan N, Sun Y, Sattentau Q, Thali M, Wu D, et al. (1998) CD4-induced conformational changes in the human immunodeficiency virus type 1 gp120 glycoprotein: consequences for virus entry and neutralization. J Virol 72: 4694–4703.
- 34. Decker J, Bibollet-Ruche F, Wei X, Wang S, Levy DN, et al. (2005) Antigenic conservation and immunogenicity of the HIV coreceptor binding site. J Exp Med 201: 1407–1419.
- 35. Forthal DN, Landucci G, Stefano-Cole K, Marthas M, Becerra JC, et al. (2006) Rhesus macaque polyclonal and monoclonal antibodies inhibit simian immunodeficiency virus in the presence of human or autologous rhesus effector cells. J Virol 80: 9217–9225.
- 36. Gomez-Roman VR, Patterson LJ, Venson D, Liewehr D, Aldrich K, et al. (2005) Vaccine-elicited antibodies mediate antibody-dependent cellular cytotoxicity correlated with significantly reduced acute viremia in rhesus macaques challenged with SIV. J Immunol 174: 2185–2189.
- 37. Aasa-Chapman MMI, Holuigue S, Aubin K, Wong M, Jones NA, et al. (2005) Detection of antibody-dependent complement-mediated inactivation of both autologous and heterologous virus in primary human immunodeficiency virus type 1 infection. J Virol 79: 2823–2830.
- 38. Huber M, Fischer M, Misselwitz B, Manrique A, Kuster H, et al. (2006) Complement lysis activity in autologous plasma is associated with lower viral loads during the acute phase of HIV-1 infection. PLoS Med 3: e441. doi:10.1371/journal.pmed.0030441.
- 39. Holl V, Peressin M, Schmidt S, Decoville T, Zolla-Pasner S, et al. (2006) Efficient inhibition of HIV-1 replication in human immature monocyte-derived dendritic cells by purified anti-HIV-1 IgG without induction of maturation. Blood 107: 4466–4474.
- 40. Holl V, Peressin M, Decoville T, Schmidt S, Zolla-Pazner S, et al. (2006) Nonneutralizing antibodies are able to inhibit human immunodeficiency virus type l replication in macrophages and immature dendritic cells. J Virol 80: 6177–6181.
- 41. Hessell AJ, Hangartner L, Hunter M, Havenith CEG, Beurskens FJ, et al. (2007) Fc receptor but not complement binding is important in antibody protection against HIV. Nature 449: 101–105.
- 42. Li M, Salazar-Gonzalez JF, Derdeyn CA, Morris L, Williamson C, et al. (2006) Genetic and neutralization properties of subtype C human immunodeficiency virus type 1 molecular env clones from acute and early heterosexually acquired infections in Southern Africa. J Virol 80: 11776–11790.
- 43. Binley J, Wrin T, Korber B, Zwick M, Wang M, et al. (2004) Comprehensive cross-subtype neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodies. J Virol 78: 13232–13252.
- 44. Li M, Gao F, Mascola JR, Stamatatos L, Polonis VR, et al. (2005) Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies. J Virol 79: 10108–10125.
- 45. Richman DD, Wrin T, Little SJ, Petropoulos CJ (2003) Rapid evolution of the neutralizing antibody response to HIV type 1 infection. Proc Natl Acad Sci U S A 100: 4144–4149.
- 46. Brown BK, Karasavvas N, Beck Z, Matyas GR, Birx DL, et al. (2007) Monoclonal antibodies to phosphatidylinositol phosphate neutralize human immunodeficiency virus type 1: Role of phosphate-binding substrates. J Virol 81: 2087–2091.
- 47. Choudhry V, Zhang M-Y, Sidrov IA, Bouma P, Cham F, et al. (2007) Cross-reactive HIV-1 neutralizing monoclonal antibodies selected by screening of an immune human phage library against an envelope glycoprotein (gp140) isolate from a patient (R2) with broadly HIV-1 neutralizing antibodies. Virology 363: 79–90.
- 48. Hu S-L (2005) Non-human primate models for AIDS vaccine research. Curr Drug Targets 5: 193–201.
- 49. Feinberg MB, Moore JP (2002) AIDS vaccine models: Challenging challenge viruses. Nat Med 8: 1–4.
- 50. Haynes B, Fleming J, St. Clair E, Katinger H, Steigler G, et al. (2005) 0 Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies. Science 308: 1906–1908.
- 51. Alam SM, McAdams M, Boren D, Rak M, Scearce RM, et al. (2007) The role of antibody polyspecificity and lipid reactivity in binding of broadly neutralizing anti-HIV-1 envelope human monoclonal antibodies 2F5 and 4E10 to glycoprotein 41 membrane proximal envelope epitopes. J Immunol 178: 4424–4435.
- 52. Fernando K, Hu H, Ni H, Hoxie JA, Weissman D (2007) Vaccine-delivered HIV envelope inhibits CD4+ T–cell activation, a mechanism for poor HIV vaccine responses. Blood 109: 2538–2544.
- 53. He B, Qiao X, Kalsse PJ, Chiu A, Chadburn A, et al. (2006) HIV-1 envelope triggers polyclonal Ig class switch recombination through CD4-independent mechanism involving BAFF and C-type lectin receptors. J Immunol 176: 3931–3941.
- 54. Binley JM, Klasse PJ, Cao Y, Jones I, Markowitz M, et al. (1997) Differential regulation of the antibody response to gag and Env proteins of human immunodeficiency virus type 1. J Virol 71: 2799–2809.
- 55. Conrad DF, Jakobsson M, Coop G, Wen X, Wall JD, et al. (2006) A worldwide survey of haplotype variation and linkage disequilibrium in the human genome. Nat Genet 38: 1251–1260.
- 56. de Bakker PIW, Burtt NP, Graham RR, Guiducci C, Yelensky R, et al. (2006) Transferability of tag SNPs in genetic association studies in multiple populations. Nat Genet 38: 1298–1303.
- 57. Zhang PF, Cham F, Dong M, Choudhary A, Bouma P, et al. (2007) Extensively cross-reactive anti-HIV-1 neutralizing antibodies induced by gp140 immunization. Proc Natl Acad Sci U S A 104: 10193–10198.