Signature-Based Small Molecule Screening Identifies Cytosine Arabinoside as an EWS/FLI Modulator in Ewing Sarcoma
Figure 4
ARA-C Inhibits Cell Viability and Induces Cell Death
(A) Four different Ewing sarcoma cell lines expressing the EWS/FLI translocation were treated with ARA-C in a dose-response series for 6 d. Cell viability was evaluated with an ATP-based assay and plotted relative to control cells. Error bars show standard deviation across four replicates.
(B) A673 cells were treated with vehicle (negative control) or puromycin (positive control) or ARA-C at 170, 340, and 680 nM for 3 d, stained with annexin V-FITC and propidium iodine, and evaluated by flow cytometry. Increasing doses of ARA-C induced increased annexin V-positive cells consistent with apoptosis.
(C) We treated four different carcinoma cell lines with ARA-C in a dose–response series for 6 d. Cell viability was evaluated with an ATP-based assay and plotted relative to control cells. Error bars show standard deviation across four replicates.