Guidelines for Figure and Table Preparation
- Select Color Mode
- Choose Figure Dimensions
- Choose Figure Text
- Choose File Format/Resolution/Size
- Upload Audio, Video, and Motion Graphics
- Format Tables
All figures will be published under a Creative Commons Attribution License, which allows them to be freely used, distributed, and built upon as long as proper attribution is given. Please do not submit any figures that have been previously copyrighted unless you have express written permission from the copyright holder to publish under the CCAL license.
As part of the process of making scientific and medical literature freely available on the Web, PLoS uses a streamlined production process that takes authors' figures straight to layout. We appreciate every effort authors put toward ensuring that their figures meet our specifications.
The figures that appear with the online version of articles are provided at the highest quality possible, so authors should submit their figures properly sized, with fonts and labels at an appropriate size, and produced in a high-resolution format. The following steps will help you meet these criteria; please read them carefully. A summary of figure requirements follows:
- Color: RGB or Grayscale
- Dimensions: Width 9 - 18 cm (or ~3.5 - 7 in), Height 1 - 24 cm (or ~0.5 - 9.5 in)
- Text: Arial font, between 8 and 12 points
- Format: TIFF or EPS (TIFF preferred)
- Resolution (for TIFF): 600 dpi
- Size: 10MB or less
PLEASE REMEMBER
Simply because a figure looks good on screen does not mean it is at an optimal resolution. For reassurance, size figure to intended dimensions, and double-check your specifications or print the image on your personal printer. The online version should look relatively similar to the personal-printer copy.
IMAGE MANIPULATION
Image files should not be manipulated or adjusted in any way that could lead to misinterpretation of the information present in the original image. Inappropriate manipulation includes but is not limited to:
- The introduction, enhancement, movement or removal of specific feature(s) within an image;
- Unmarked grouping of images which should otherwise have been presented separately (for example, from different parts of the same gel, or from different gels, fields, or exposures);
- Adjustments of brightness, contrast, or color balance which obscure, eliminate, or misrepresent any information.
Digital images in manuscripts that are close to being accepted for publication may be scrutinized for any indication of improper manipulation. If any evidence is found of inappropriate manipulation we reserve the right to ask for original data and if that is not satisfactory may decide not to accept the manuscript.
We are grateful to staff at the Journal of Cell Biology (Rockefeller University Press), for their help in establishing these guidelines and procedures (http://www.jcb.org/misc/ifora.shtml#image_aquisition).
Step 1: Select Color Mode
Your images should be in either RGB or grayscale. Please adjust your color settings before beginning your work.
Step 2: Choose Figure Dimensions
After removing all excess white space, figures should have a width between 9 cm and 18 cm. Figures cannot have a height of more than 24 cm.
- Width: 9 - 18 cm (or ~3.5 - 7 in)
- Height: 1 - 24 cm (or ~0.5 - 9.5 in)
All figures will be left-aligned on the page or column, so please design accordingly.
Left-Aligned Figure
Step 3: Choose Figure Text
If your figure has multiple parts, please label in capital letters as A, B, C, etc., using Arial bold font, 12 points. Do NOT use any punctuation (no periods or brackets)! Any other figure text must be in Arial font, between 8 and 10 points.
Always embed fonts or create outlines when using vector file types (EPS format). In addition to special symbols and Greek characters not reproducing properly, we cannot guarantee that your figure will be published using your intended font, unless you embed or create outlines.
Embedding Fonts: To embed your fonts in Adobe Illustrator: When saving an EPS file, make sure that the Embed Fonts option is selected in the Save or Save As dialogue box.
Creating Outlines: To create outlines of your text in Adobe Illustrator: Using the Selection tool, select the text you want to outline. Then choose Type > Create Outlines (Shift + Control + O on PC, Shift + Apple + O on Mac).
Step 4. Choose File Format/Resolution/Size
We accept figures in either TIFF or EPS format; however, authors should note that EPS files will be automatically converted to TIFF prior to publication.
TIFF files must have a minimum resolution of 300 dpi, and any images placed within EPS files must also have an original resolution of at least 300 dpi.
Please remember that the quality of your figures will only be as good as the lowest-resolution element placed in them. In other words, if you use a 72 dpi line graph and make it a 600 dpi TIFF, the image will still print out as a 72 dpi image. The figure should not look fuzzy, jagged, pixilated, or grainy at intended print size.
Each figure (including all supporting figures) should be under 10 MB to optimize online accessibility.
If your figure is assembled from multiple images, please embed images, group objects or flatten layers, and flatten transparencies.
When saving as TIFF files, please choose compression option LZW (this will greatly reduce file size while retaining quality). Some programs may downsample your images to low resolution. Do not use the "optimize for web" wizard for any figures.
If your source files are not TIFF or EPS, they need to be converted.
- JPG files: Open your JPG file in Photoshop. Do a Save As TIFF using LZW compression.
- PDF files: Open the PDF file in Photoshop as a high resolution file (600dpi or higher). Re-save the file as a TIFF using LZW compression.
- PPT files: Convert your PPT file to a PDF using the Adobe PDF option in PowerPoint. On a PC, this option is in the topmost bar of your program; select the first item on the drop-down menu called Convert to Adobe PDF. On a Mac, select the Adobe PDF icon in your toolbar. Once the PDF has been created, follow the steps above for converting a PDF.
Please contact figures [at] plos.org if you need help converting your files.
Step 5: Upload Audio, Video, and Motion Graphics
We encourage authors to submit multimedia files that are crucial to the conclusions of the paper. Multimedia files should be smaller than 10 MB in size because of the difficulties that some users will experience in loading or downloading files. Preferred formats are:
- Audio: MP3
- Video: MOV, progressive download, 320x240px frame size
- Flash: SWF
Step 6: Format Tables
Tables submitted for production should be included at the end of the article DOC or RTF file. For LaTeX submissions, table files should be uploaded individually into the online submission system. Tables that will be Supporting Information files can be submitted in any allowed format: Word, Excel, PDF, PPT, JPG, EPS, or TIFF.
Title and footnotes
Each table needs a concise title of no more than one sentence. The legend and footnotes should be placed below the table. Footnotes can be used to explain abbreviations.
Specifications
Tables that do not conform to the following requirements may give unintended results when published. Problems may include movements of data (rows or columns), loss of spacing, or disorganization of headings. Note: Multi-part tables with varying numbers of columns or multiple footnote sections should be divided and renumbered as separate tables.
Table requirements:
- Cell-based (e.g., created in Word with Tables tool or in Excel).
- Editable (i.e., not graphic object).
- Heading/subheading levels in separate columns.
- Size no larger than one printed page (7 in x 9.5 in). Larger tables can be published as online supporting information.
- No returns, tabs, or merged cells or rows.
- No color, shading, lines, or rules.
- No inserted text boxes or pictures.
- No tables within tables.