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Correction: Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in Arabidopsis thaliana

  • Qi Li,
  • Xiaokang Wang,
  • Han Sun,
  • Jun Zeng,
  • Zhendong Cao,
  • Yan Li,
  • Weiqiang Qian

Correction: Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in Arabidopsis thaliana

  • Qi Li, 
  • Xiaokang Wang, 
  • Han Sun, 
  • Jun Zeng, 
  • Zhendong Cao, 
  • Yan Li, 
  • Weiqiang Qian
PLOS
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The second panel from the bottom of the left side of Fig 1D, is missing. The authors have provided a corrected version here.

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Fig 1. Protein-protein interactions between IDM1, IDM2, IDL1 and MBD7.

(A) Yeast two-hybrid assays. Yeast cells carrying different fusion protein combinations are listed on the left. Yeast cells expressing the indicated proteins from the pGBK-T7 (BD) and pGAD-T7 (AD) vectors were plated onto medium lacking Leu and Trp (SD-LT) (left) or medium lacking Leu, Trp, Ade and His (SD-LTHA) (right). (B) Pull-down assays showing that IDM2, IDL1 and MBD7 interact with each other. (C) Split-luc assays showing that IDL1 and MBD7 can interact with IDM2 in N. benthamianaleaves. Three biological replicates were performed, and similar results were obtained. (D) Co-immunoprecipitation of MBD7 with IDM2 or IDL1 in tobacco leaves. MYC-tagged IDM2 and GFP-tagged IDM1 were transiently expressed in N. benthamiana leaves. Anti-GFP was used for immunoprecipitation (IP); anti-MYC and anti-GFP were used for immunoblotting; Input, total protein before immunoprecipitation. Transgenic plants expressing MBD7-Myc or IDM1-HA-YFP under their native promoters and their F1 offspring were used for co-IP.

https://doi.org/10.1371/journal.pgen.1005380.g001

Reference

  1. 1. Li Q, Wang X, Sun H, Zeng J, Cao Z, Li Y, et al. (2015) Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in Arabidopsis thaliana. PLoS Genet 11(5): e1005210. pmid:25933434