SIRT1 is a metabolic sensor and regulator in various mammalian tissues and functions to counteract metabolic and age-related diseases. Here we generated and analyzed mice that express SIRT1 at high levels specifically in skeletal muscle. We show that SIRT1 transgenic muscle exhibits a fiber shift from fast-to-slow twitch, increased levels of PGC-1α, markers of oxidative metabolism and mitochondrial biogenesis, and decreased expression of the atrophy gene program. To examine whether increased activity of SIRT1 protects from muscular dystrophy, a muscle degenerative disease, we crossed SIRT1 muscle transgenic mice to mdx mice, a genetic model of Duchenne muscular dystrophy. SIRT1 overexpression in muscle reverses the phenotype of mdx mice, as determined by histology, creatine kinase release into the blood, and endurance in treadmill exercise. In addition, SIRT1 overexpression also results in increased levels of utrophin, a functional analogue of dystrophin, as well as increased expression of PGC-1α targets and neuromuscular junction genes. Based on these findings, we suggest that pharmacological interventions that activate SIRT1 in skeletal muscle might offer a new approach for treating muscle diseases.
Skeletal muscle has a central role in body posture, mobility and whole-body metabolism. SIRT1 is an enzyme expressed in skeletal muscle, as well as in most mammalian tissues, and has been shown to sense metabolic cues from the environment and mediate changes in these tissues, counteracting age and metabolic diseases. Here we generated and studied mice that express high levels of SIRT1 in skeletal muscle. We found that increased levels of SIRT1 in skeletal muscle led to gene expression changes similar to those that normally occur with endurance exercise. We also observed that SIRT1 overexpression counteracts muscle atrophy, a hallmark of aging muscle, and the muscle degenerative disease Duchenne muscular dystrophy (DMD). DMD is a debilitating disease caused by a mutation in the structural protein dystrophin. SIRT1 overexpression ameliorated the pathophysiology of DMD disease in a mouse model. Our results offer the hope that drugs that constitutively activate the enzymatic activity of SIRT1 might be used to cure muscle degenerative diseases.
Citation: Chalkiadaki A, Igarashi M, Nasamu AS, Knezevic J, Guarente L (2014) Muscle-Specific SIRT1 Gain-of-Function Increases Slow-Twitch Fibers and Ameliorates Pathophysiology in a Mouse Model of Duchenne Muscular Dystrophy. PLoS Genet 10(7): e1004490. https://doi.org/10.1371/journal.pgen.1004490
Editor: Johan Auwerx, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland
Received: May 8, 2013; Accepted: May 21, 2014; Published: July 17, 2014
Copyright: © 2014 Chalkiadaki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: AC was supported by a postdoctoral fellowship from American Heart Association. MI was supported by Manpei Suzuki Diabetes Foundation. LG is supported by the NIH and the Glenn Foundation for Medical Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Skeletal muscle has a central function in body stature and motility, as well as in energy storage, energy consumption, and whole-body metabolism. The various skeletal muscle groups consist of heterogeneous and specialized myofibers and are responsive and highly adaptable to contractile activity, nutrient availability, and hormones . The different fiber types are characterized by specific biochemical, physiological, and metabolic parameters, which determine the function, size, metabolism, and fatigue resistance of each muscle group . The myofibers are classified in two major types–the slow- and fast-twitch– with distinct contractile and metabolic properties. The slow-twitch myofibers contain mainly the type I myosin heavy chain isoform, are rich in mitochondria, and exhibit oxidative metabolism. The fast-twitch myofibers contain type IIa, IId/x, and IIb myosin heavy chain isoforms, are mainly glycolytic, perform quick contractions, and are required for movements involving strength and speed, but they are easily fatigued   . Most muscles consist of a mixture of fiber types, and the ratio is altered by exercise, or various systemic conditions, such as diabetes, cancer, and aging . Calorie restriction, fasting, and exercise induce changes in skeletal muscle by transforming the myofibers from glycolytic to more oxidative forms rendering them more resistant to fatigue and atrophy  , . Conversely, aging is associated with skeletal muscle atrophy, characterized by a progressive loss of oxidative fibers .
SIRT1, the mammalian orthologue of the yeast NAD+-dependent protein deacetylase Sir2 (silent information regulator 2), is expressed in various mammalian tissues, including skeletal muscle, and serves as a sensor and regulator of the energetic status of the cell, counteracting metabolic and age-related diseases , . Under conditions of low glucose availability and increased energy demands, such as fasting, calorie restriction, and exercise, SIRT1 is induced in skeletal muscle and mediates mitochondrial biogenesis and fatty acid oxidation by deacetylating and regulating the activity of the transcriptional coactivator peroxisome-proliferator-activated receptor-gamma coactivator-1 α (PGC-1α) or forkhead box O (FOXO) transcription factors , , . PGC-1α is a master regulator of mitochondrial gene expression , and transgenic overexpression has been shown to activate switching from fast-twitch to slow-twitch oxidative fibers in skeletal muscle, provide resistance to electrical stimulated fatigue  and protect from atrophy and metabolic disease during aging ,  . In aged muscle, NAD+ levels and SIRT1 activity decline, with a subsequent decrease in the expression of mitochondrial-encoded genes and mitochondrial homeostasis .
PGC-1α overexpression in skeletal muscle also ameliorates the phenotype of the X-linked recessive, muscle wasting disease Duchenne muscular dystrophy (DMD) . DMD arises from a frameshift mutation in the gene dystrophin and leads to rapid degeneration of heart and skeletal muscle, causing disability and death by adolescence or young adulthood . The dystrophic muscle is characterized by massive degeneration and necrosis of the damaged myofibers. It was observed that in DMD patients the fast-twitch fibers are more prone to damage, whereas the slow-twitch are relatively spared . Active muscle regeneration–manifested by centrally localized nuclei– initially compensates for the degeneration but progressively the damaged muscle is replaced by connective and adipose tissues . In normal muscle, dystrophin and the dystrophin-associated protein complex form a link between the intracellular actin based cytoskeleton and the extracellular matrix. Dystrophin is enriched at the junctions of muscle fibers and tendons and at the junctions of motor neurons with the muscle fibers (neuromuscular junction) , . Lack of dystrophin protein leads to membrane destabilization and increased fragility, especially during intense contractile activity. PGC-1α stabilizes the weak cell membrane of the dystrophic myofiber by activating the neuromuscular junction (NMJ) gene program . A strategy proposed to alleviate DMD involves the upregulation of utrophin , an autosomal ortholog of dystrophin, which partially compensates for dystrophin absence , , . Because utrophin is expressed at higher levels in slow-twitch, oxidative fibers, it has been proposed that some of the effect of PGC-1α gain-of-function may be because it triggers conversion of fast-twitch to slow-twitch fibers .
In the current study, we explored the role of the metabolic regulator SIRT1 in skeletal muscle physiology under normal conditions and in the DMD model. We show that increased levels of SIRT1 in skeletal muscle drive a switch to slow-twitch fibers, reduce the muscle atrophy gene expression program, and ameliorate the DMD phenotype. In contrast, deletion of muscle SIRT1 exerts relatively minor phenotypes, suggestive of the presence of redundant mechanisms.
Transgenic expression of SIRT1 in skeletal muscle induces a switch to slow-twitch oxidative fibers
To study the role of SIRT1 in skeletal muscle physiology and in disease conditions, we generated skeletal muscle-specific SIRT1 overexpressing mice. The transgenic (Tg) mice were generated by injecting oocytes with a construct containing the cDNA of mouse SIRT1 under the control of muscle creatine kinase promoter (MCK) . We obtained three lines that all express higher than wild-type (WT) levels of SIRT1 in skeletal muscle: Tg-4140 (Figure 1A and Figure S1A), Tg-4145, and Tg-4311 (Figure S1B and Figure S1C). In most of the studies below we used the Tg-4140 line, unless otherwise indicated. The Tg mice were born to Mendelian ratios and their gross phenotype appeared normal. Whole-body weight of Tg-4140 mice was comparable to WT sibling controls, but muscle weight and muscle/body weight ratio were approximately 40% reduced compared to WT controls (Figure 1B). Histological analysis of WT and Tg-4140 gastrocnemius muscle by hematoxylin and eosin staining showed that Tg-4140 muscle had normal appearance (Figure 1C). We measured the cross-sectional area of WT and Tg-4140 fibers of gastrocnemius muscle and observed that Tg-4140 fibers were significantly smaller than WT (Figure 1C), which could explain why their muscles weigh less.
(A) Western blot in tissue protein homogenates prepared from WT and transgenic gastrocnemius muscle (line 4140). All following experiments were performed using line Tg-4140, unless otherwise indicated. (B) Gastrocnemius muscle weight (of both hindlimbs) and muscle/body weight ratio of WT and Tg-4140 mice at 10 weeks of age (n = 7–10). (C) Representative H&E staining of gastrocnemius muscle from WT and transgenic mice and quantification of fiber size (arbitrary units) (700–1000 fibers/genotype, n = 3). Data are expressed as mean +/− s.e.m. ***p<0.001 by two-tailed unpaired Student's t test.
To examine whether SIRT1 overexpression in skeletal muscle activates the muscle wasting gene program, resulting in increased proteasomal degradation and therefore smaller muscles, we measured the expression levels of atrophy genes by quantitative RT-PCRs, under basal and atrophy-inducing conditions. We observed that under basal conditions the expression levels of the two E3 ubiquitin ligases, hallmarks of skeletal muscle atrophy, MAFBx and MuRF1, were actually reduced in Tg-4140 muscles compared to WT (Figure 2A). To induce muscle atrophy we subjected WT and Tg-4140 mice to either 24 hr fasting (Figure 2A) or 3 days of disuse induced by denervation after sectioning the sciatic nerve (Figure 2B). We verified that MAFBx and MuRF1 genes were strongly induced in WT muscles under both conditions of atrophy (Figure 2A and 2B), as previously described , . However, the induction of the atrophy markers in Tg-4140 was significantly less compared to WT (Figure 2A and 2B). It was previously shown that the FOXO transcription factors are induced in fasting atrophy and are necessary for the atrophy gene program in skeletal muscle , . So we tested whether SIRT1 overexpression affects the induction of FOXO transcription factors after fasting, and we observed that FOXO1 was not induced in Tg-4140 muscle compared to WT after 24 hr fasting, whereas the induction of FOXO3 mRNA was only weakly affected (Figure 2C). In conclusion, SIRT1 overexpression does not induce muscle wasting; on the contrary, it counteracts the atrophy gene program.
(A) Relative mRNA levels of MAFBx and MuRF-1 atrophy genes in gastrocnemius muscle of WT and Tg-4140 mice fed or fasted for 24 hrs (n = 3–5). (B) Relative mRNA levels of MAFBx and MuRF-1 atrophy genes in gastrocnemius muscle of WT and Tg-4140 mice, which underwent mock surgery (control) or were denervated for 3 days (n = 3–5). (C) Relative mRNA levels of FOXO1 and FOXO3 transcription factors in gastrocnemius muscle of WT and Tg-4140 mice fed or fasted for 24 hrs (n = 3–5). Data are expressed as mean +/− s.e.m. *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.
A role of SIRT1 in skeletal muscle physiology was suggested by the induction of its activity during exercise and calorie restriction , , . Both conditions drive fiber type switch, mitochondrial biogenesis, and more oxidative metabolism . So overexpression of SIRT1 could alter the composition of fibers, inducing the formation of more oxidative, slow-twitch fibers, which are smaller in size. To test this hypothesis, we measured the expression levels of various fiber type markers by quantitative RT-PCRs in gastrocnemius muscle of WT and Tg-4140 mice. We observed a switch towards more oxidative slow-twitch type in Tg-4140 muscle as manifested by an increase in markers of slow-twitch and more oxidative fibers (troponin slow) and a concomitant decrease in fast-twitch and more glycolytic myofibers (troponin fast) (Figure 3A). In addition, we measured the relative gene expression levels of myosin heavy chain isoforms, and we observed an increase in MHC-I and in the isoforms of MHC-2 (2x and 2A) associated with slow-twitch oxidative fibers  and a concomitant reduction in MHC-2B isoform associated with fast-twitch fibers  (Figure 3B). Histological analyses of gastrocnemius/soleus muscle enzymatically stained for the mitochondrial enzyme succinate dehydrogenase (SDH) (Figure 3C and Figure S2A) and cytochrome oxidase (COX) (Figure 3D and Figure S2B), as well as measurements of mitochondrial DNA content (Figure 3E), verified that transgenic muscle contains more oxidative fibers and increased mitochondrial activity. In addition, myosin ATPase activity staining showed a 50% increase in type I fibers in transgenic gastrocnemius muscle (Figure S2C). Consistent with the fiber type switch and increased mitochondrial content and activity, genes encoding transcription factors associated with increased mitochondrial gene expression, such as PGC-1α, TFAM, and PPARα, mitochondrial proteins such as cytochrome c and some of the electron transport chain proteins were upregulated in Tg-4140 muscle (Figure 3F, 3G, and 3H), as well as in muscle of Tg-4311 and Tg-4145 mice (Figure S1D, S1E, S1F and S1G). PGC-1α is central in the regulation of mitochondrial biogenesis in skeletal muscle, and SIRT1 is known to activate its transcriptional activity by deacetylation , . We examined whether PGC-1α protein is differentially acetylated in Tg-4140 muscle by immunoprecipitation followed by western blot (Figure 3I). First, we observed that PGC-1α protein is induced in Tg-4140 muscle (Figure 3I), consistent with the increased RNA levels we observed, and a previously reported positive autoregulation of PGC-1α promoter by PGC-1α protein . Immunoprecipitation of PGC-1α from WT and transgenic muscle followed by western blot with anti-acetyl lysine antibodies showed that the levels of acetylation of PGC-1α in transgenic muscle are reduced, in agreement with the known role of SIRT1 in PGC-1α acetylation status and activity (Figure 3I). From these data we concluded that SIRT1 overexpression results in fiber type switch towards more oxidative metabolism and increased mitochondrial activity.
(A) Relative mRNA levels of troponin slow and troponin fast genes in gastrocnemius muscle of WT and Tg-4140 mice (8–10 weeks old, n = 3–5). (B) Relative mRNA levels of myosin heavy chain 2B, 2x, 2A, and I in gastrocnemius muscle of WT and Tg-4140 mice (n = 3–5). (C) Representative SDH activity staining of cross-sections of gastrocnemius/soleus (upper panels) and gastrocnemius (lower panel) muscle of WT and Tg-4140 mice. Quantitation is shown in Figure S2A. (D) Representative COX activity staining of cross-sections of gastrocnemius muscle of WT and Tg-4140 mice. Quantitation is shown in Figure S2B. (E) Relative mitochondrial DNA of indicated genes normalized to actin (10–12 weeks old, n = 4). (F) Relative mRNA levels of TFAM, PPARα, PPARδ, and PGC-1α in gastrocnemius muscle of WT and Tg-4140 mice (n = 3–5). (G) Relative mRNA levels of mitochondrial-expressed electron transport chain (ETC) genes in gastrocnemius muscle of WT and Tg-4140 mice (10–12 weeks old, n = 3–5). (H) Relative mRNA levels of nuclear-expressed electron transport chain (ETC) genes in gastrocnemius muscle of WT and Tg-4140 mice (10–12 weeks old, n = 3–5). (I) Lysine acetylation levels of PGC-1α (IB: ac-Lys) in protein extracts prepared from gastrocnemius muscle of WT and Tg-4140 and immunoprecipitated by PGC-1α specific antibodies (IP: PGC-1α). Data are expressed as mean +/− s.e.m. *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.
Skeletal muscle consumes large amounts of energy in the body by glucose uptake. Because SIRT1 has a central role in energy sensing and metabolic regulation, we tested whether SIRT1 overexpression in the muscle affects whole-body glucose homeostasis and response to fasting. We found that fed and fasting glucose and insulin levels in the blood of Tg-4140 mice were comparable to WT controls (Figure 4A). In addition, we measured the levels of genes that are known to respond to fasting and we found that Tg-4140 muscle adapts to fasting as efficiently as WT (Figure 4B, 4C, 4D, 4E, 4F, and 4G). Previous evidence showed that there is an interdependence of the energy sensor AMP-activated protein kinase (AMPK) and SIRT1 in skeletal muscle, with AMPK upregulating the NAD+ levels and SIRT1 activity upon fasting or exercise , , . We examined whether overexpression of SIRT1 alters AMPK activity under basal fed conditions by western blot analysis using phospho-specific antibodies, and we observed that the phosphorylation status of the catalytic subunit AMPKα at Thr172, which is required for AMPK activation, remains unaltered in Tg-4140. The mammalian target of rapamycin (mTOR) is also a central energy sensor and functions to balance nutrient availability and cell growth . In Tg-4140 muscle, the phosphorylation of the downstream target of mTOR pathway p70 S6 kinase (p70-S6K) at Thr389 is increased, suggesting that mTOR activity is induced (Figure 4H). Future studies will further investigate the mechanism by which SIRT1 overexpression leads to activation of mTOR pathway. In conclusion, overexpression of SIRT1 in skeletal muscle drives the formation of more oxidative fibers but cannot alter basal whole-body glucose homeostasis and the response to fasting.
(A) Blood glucose and plasma insulin levels in fed or after overnight fasting of WT and Tg-4140 mice (8–10 weeks old, n = 6–10). (B–G) Relative mRNA levels of GLUT4, PDK4, mitofusin, mCPT1, ERRα, and MCAD genes in gastrocnemius muscle of fed and fasted WT and Tg-4140 (10–12 weeks old, n = 4). (H) Western blot analyses in gastrocnemius muscle protein extracts prepared from gastrocnemius muscle of WT and Tg-4140 (10–12 weeks old). Data are expressed as mean +/− s.e.m. *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.
Loss of SIRT1 from skeletal muscle does not affect oxidative metabolism or myofiber composition
To examine whether SIRT1 activity is necessary for oxidative metabolism and fiber type composition in skeletal muscle, we generated muscle-specific SIRT1 knockout (MckKO) mice, by crossing mice expressing the cre recombinase under the control of MCK promoter  to mice carrying the floxed SIRT1 allele . The loxP sites flank the exon 4 of SIRT1 gene, which corresponds to the catalytic domain of the enzyme, so cre-mediated excision results in a smaller SIRT1 protein that lacks its enzymatic activity (Figure 5A). The appearance and weight of MckKO muscle were comparable to WT controls (Figure 5B). Blood glucose levels were slightly elevated in MckKO mice, but insulin levels were normal (Figure 5C). Expression levels of troponin isoforms (Figure 5D), myosin heavy chain isoforms (Figure 5E), and mitochondrial transcription factors (Figure 5F) did not differ between WT and MckKO muscles by quantitative RT-PCRs. Thus SIRT1 loss from skeletal muscle did not affect the expression levels of mitochondrial transcription factors or myofiber type composition, which is in agreement with recently published papers , . We challenged MckKO and WT sibling controls by forced treadmill exercise to exhaustion. We employed a mild running protocol and we observed no difference between WT and MckKO mice. However, when we used a more intense exercise protocol we observed that MckKO mice were exhausted faster and ran shorter distance compared to WT mice (Figure 5G). A possible explanation for intolerance in exercise is defective mitochondrial function. To further investigate this possibility, we measured the expression levels of mitochondrial- and nuclear-expressed electron transport chain (ETC) genes, and we found that the mitochondrial- but not the nuclear- expressed ETC genes are slightly but significantly reduced in MckKO muscle (Figure 5H and 5I). Our observations are consistent with recent findings showing that SIRT1 regulates mitochondrial-encoded ETC genes  and suggest that MckKO mice are more sensitive to fatigue after acute exercise, possibly because of reduced expression of mitochondrial-expressed ETC genes.
(A) Western blot in tissue protein homogenates prepared from WT and muscle-specific SIRT1 knockout (MckKO) gastrocnemius muscle. The arrow shows the faster migrating SIRT1 protein lacking the catalytic domain (ΔExon4). Relative RNA levels of SIRT1 transcript using SIRT1 exon 4 specific primers. (B) Gastrocnemius muscle weight (of one hindlimb) of WT and MckKO mice at 10 weeks of age (n = 7–10). (C) Blood glucose and plasma insulin levels in WT and MckKO mice after overnight fasting (8–10 weeks old, n = 6–10). (D) Relative mRNA levels of troponin slow and troponin fast genes in gastrocnemius muscle of WT and MckKO mice (8–10 weeks old, n = 3–5). (E) Relative mRNA levels of myosin heavy chain 2B, 2x, 2A, and I in gastrocnemius muscle of WT and MckKO mice (n = 3–5). (F) Relative mRNA levels of TFAM, PPARα, and PGC-1α in gastrocnemius muscle of WT and MckKO mice (n = 3–5). (G) Distance run in treadmill exercise by WT and MckKO mice (12–14 weeks old, n = 10–12). (H) Relative expression levels of mitochondrial-expressed electron transport chain (ETC) genes (10–12 weeks old, n = 4). (I) Relative expression levels of nuclear-expressed ETC genes (10–12 weeks old, n = 4). Data are expressed as mean +/− s.e.m. *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.
Prompted by our observations that SIRT1 overexpression results in lower expression levels of atrophy genes, under basal and atrophy inducing conditions, we examined the levels of these genes in gastrocnemius muscle from MckKO mice. Quantitative RT-PCR analyses showed that absence of SIRT1 did not affect the levels of MAFBx and MuRF1 under basal or atrophy-inducing conditions (Figure S3A and S3B). In summary, deletion of SIRT1 in muscle exerts only a subtle phenotype under the conditions tested.
SIRT1 transgenic mice express high levels of utrophin and neuromuscular junction genes in skeletal muscle
Several lines of evidence led us to hypothesize that SIRT1 overexpression in muscle could counteract the muscular degenerative disease DMD. For example, it is known that slow-twitch, oxidative fibers are less prone to degeneration compared to fast-twitch glycolytic fibers . As SIRT1 transgenic muscles contain more slow-twitch fibers (Figure 3), transgenic mice might be protected from muscle degeneration. In addition, slow-twitch fibers express higher levels of utrophin, the functional analogue of dystrophin that could compensate to some extent for dystrophin's loss in DMD. In addition, SIRT1 overexpression leads to increased levels of PGC-1α, known to protect against DMD . We thus crossed SIRT1 Tg-4140 and Tg-4145 mice to mdx mouse model  (Figure 6A), which is a severe model of Duchenne muscular dystrophy. We measured body and muscle weights of all four groups (WT, Tg, mdx, and mdx;Tg). Our results showed that SIRT1 overexpression in mdx muscle reverses the characteristic muscle hypertrophy of dystrophic muscle caused by vigorous regeneration following muscle damage and necrosis (Figure 6B). We tested whether overexpression of SIRT1 in the mdx mouse also leads to increased levels of PGC-1α, as in the WT genetic background, and we observed that both RNA (Figure 6C) and protein levels (Figure 6A) are increased. To verify that SIRT1 overexpression affects the expression levels of utrophin, we performed quantitative RT-PCR analysis and we observed that utrophin levels are increased more than 2-fold in gastrocnemius muscle of Tg-4140, 1.5 fold in mdx;Tg-4140 (Figure 6D), and 1.5 fold in Tg-4145 (Figure S4A) mice.
(A) Western blot in tissue protein homogenates prepared from gastrocnemius muscles of mdx and mdx mice crossed to Tg-4140 (mdx;TG) (B) Body weight, gastrocnemius muscle weight (of both hindlimbs) and muscle/body weight ratio of WT, Tg-4140, mdx, and mdx;Tg-4140 mice at 10–14 weeks of age (n = 5–10). (C–J) Relative mRNA levels of PGC-1α, utrophin, acetylcholine receptor (AchR) subunits α, δ, ε, and Musk, Erbb1, and Raspn in gastrocnemius muscle of WT, Tg-4140, mdx, and mdx;Tg-4140 mice (n = 3–5). Data are expressed as mean +/− s.e.m. *p<0.05, ** p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.
PGC-1α, a bona-fide target of SIRT1, stimulates the neuromuscular gene program (NMJ), as a coactivator of GABP transcription factor, and counteracts the abnormalities of NMJ morphology in a muscular dystrophy model . Thus, we examined whether SIRT1 overexpression in skeletal muscle also leads to increased levels of NMJ genes, and we observed that transcripts of the acetylcholine receptor subunits, and components of the acetylcholine signaling, were all expressed at dramatically higher levels in Tg-4140 and mdx;Tg-4140 muscles compared to WT and mdx muscles, respectively (Figure 6E, 6F, 6G, 6H, 6I, and 6J). Intriguingly, mdx mice themselves showed a modest upregulation of the three AchR genes and Erbb1 gene– but not the other NMJ genes– compared to WT, in agreement to previous observations , possibly representing a compensatory response of acetylcholine receptors in mdx mice.
SIRT1 overexpression in skeletal muscle alleviates the phenotype of muscular dystrophy
Our observations that SIRT1 transgenic muscle consists of higher percentage of slow-twitch oxidative myofibers and express high levels of utrophin and NMJ genes prompted us to test whether SIRT1 overexpression protects from muscular dystrophy. We first measured the levels of serum creatine kinase (CK), a hallmark of damaged muscles, in young adult WT, Tg, mdx, and mdx/Tg mice. As expected, we observed a large increase in CK activity in mdx mice compared to WT mice. Critically, we found that overexpression of SIRT1 in Tg-4140 and Tg-4145 mice resulted in a ∼50% reduction of CK enzymatic activity in the mdx genetic background (Figure 7A and Figure S4B). Another hallmark of dystrophic muscles is the large percentage of myofibers with centrally localized nuclei, indicative of regenerating tissue after damage, and large areas of infiltrating macrophages and fibroblasts. We compared the percentage of non-muscle cells infiltrating the gastrocnemius muscle in mdx;Tg-4140 muscle with mdx muscle, by hematoxylin and eosin staining. The mdx muscle contains approximately 8% of fibrotic tissue, whereas SIRT1 overexpression in mdx;Tg-4140 mice resulted in a reduction of fibrosis to about 1% (Figure 7B). To further assess the extent of damaged myofibers, we injected intraperitoneally Evans Blue dye, which only penetrates and stains damaged cells. Evans Blue dye stained ∼25% of myofibers of mdx mice, whereas overexpression of SIRT1 in mdx;Tg-4140 mice reduced the stained myofibers to ∼8% (Figure 7C).
(A) Serum creatine kinase activity in WT, Tg-4140, mdx, mdx;Tg-4140 mice (8–10 weeks old, n = 10). (B) Representative H&E staining of gastrocnemius muscle from mdx and mdx;Tg-4140 mice and quantification of damaged area (10 sections/genotype, n = 3). (C) Representative Evans Blue staining of gastrocnemius muscle from mdx and mdx;Tg-4140 mice and quantification of staining area (10 sections/genotype, n = 3). (D) Distance run in treadmill exercise by WT and Tg-4140 mice, and mdx and mdx;Tg-4140 mice (12–14 weeks old, n = 10–12). (E) Relative mRNA levels of SIRT1 in gastrocnemius muscle of WT and mdx mice (10–12 weeks old, n = 4). Western blot analysis of protein extracts prepared from gastrocnemius muscle of WT and mdx mice. (F–I) Relative mRNA levels of NAMPT, NMNAT1, NMNAT2, and NMNAT3 in gastrocnemius muscle from WT, Tg-4140, mdx and mdx;Tg-4140 mice (n = 3–5). (J) NAD+ levels in gastrocnemius muscle from WT, Tg-4140, mdx and mdx;Tg-4140 mice (n = 3–5). Data are expressed as mean +/− s.e.m. *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.
Individuals with DMD have weak muscles, which are quickly exhausted. Similarly, the mdx mice when forced to run are fatigued much faster compared to WT controls, as the mdx fibers are more vulnerable . To assess the effects of SIRT1 overexpression on physical performance of mdx mice, we challenged mdx and mdx;Tg-4140 mice with treadmill exercise to exhaustion. While the mdx mice ran on average 100 m, the mdx;Tg-4140 mice ran significantly more, ∼250 m, a distance comparable to WT mice (Figure 7D). Overall, these results suggest that SIRT1 overexpression in skeletal muscle dramatically improves the pathophysiology of the muscular dystrophic phenotype.
Next, we asked whether SIRT1 levels decline in mdx muscle. We assessed SIRT1 RNA and protein levels, and we observed that SIRT1 is expressed in WT and mdx muscle at comparable levels (Figure 7E). Since NAD+ is a rate limiting co-substrate for SIRT1, it is possible that NAD+ levels are altered in mdx muscle affecting SIRT1 activity. We assessed the expression levels of the enzymes in the NAD+ biosynthetic pathway and we observed that NAMPT (mitochondrial), NMNAT1 (nuclear), and NMNAT3 (mitochondrial), are reduced in mdx muscle, suggesting that NAD+ levels are also reduced (Figure 7F, 7G, 7I). We did not observe any significant difference in NMNAT2 (Golgi) (Figure 7H). We directly measured NAD+ levels in WT and mdx muscle and we observed that the dystrophic muscle has lower levels of NAD+, in agreement to the reduced expression levels of NAD+ biosynthetic enzymes (Figure 7J). Interestingly, we observed that SIRT1 overexpression induces the expression of NAMPT, NMNAT1, and NMNAT3 in WT and mdx genetic background. Conversely, overexpression of SIRT1 results in moderate reduction of cellular NAD+ levels in WT genetic background, probably caused by increased consumption. The increase in NAD+ synthetic enzymes may be a compensatory mechanism for increased NAD+ consumption in transgenic mice. Overall our results show that the expression levels of NAD+ biosynthetic enzymes and NAD+ concentration are reduced in dystrophic muscle, suggesting that the activity of NAD+-dependent enzymes, such as SIRT1, is downregulated. In SIRT1 transgenic mice, the reduced NAD+ likely corresponds to increased SIRT1 activity and protein levels.
Finally, we examined whether absence of SIRT1 worsened the dystrophic phenotype of mdx mice by crossing them to mice lacking SIRT1 from muscle (MckKO). We found that CK activity in mdx;MckKO mice was similar to mdx sibling controls (Figure S4C), as was the endurance of these mice when subjected to treadmill exercise to exhaustion (Figure S4D). In agreement with these results, SIRT1 loss does not affect the expression levels of neuromuscular junction genes in WT or mdx mice (Figure S4E and S4F). These results are consistent with the observations that knocking out SIRT1 in WT genetic background exerts only a subtle phenotype on mice fed normal chow diet , , , .
SIRT1 is an important metabolic regulator in mammals; it is induced under energy-limiting conditions in various tissues, including skeletal muscle, and deacetylates and regulates the activity of target proteins , . Here we generated SIRT1 muscle overexpressing and knockout mice to study the role of SIRT1 in skeletal muscle in vivo. Our data show that SIRT1 overexpression results in a fast-to-slow fiber type switch that translates into an increase in oxidative fibers. These observations correlate with higher expression of PGC-1α in SIRT1 Tg muscle and are in agreement with previous observations made with PGC-1α transgenic mice. A number of studies have shown that SIRT1 deacetylates and positively regulates PGC-1α in cell culture systems and under fasting or exercise conditions in vivo , , . Overexpression of PGC-1α in skeletal muscle regulates mitochondrial biogenesis, activates oxidative metabolism, drives fast-to-slow fiber switch  and protects from muscular atrophy and muscular dystrophy , . Moreover, muscle specific PGC-1α KO mice showed a shift from oxidative type I and IIa toward type IIx and IIb muscle fibers, reduced endurance capacity in treadmill exercise and increased muscle damage , .
However, recent papers showed that muscle PGC-1α is dispensable for voluntary exercise-induced mitochondrial biogenesis , , pointing that there are other unidentified factors and pathways that regulate mitochondrial biogenesis during exercise. Similarly, our data regarding SIRT1 loss-of-function from skeletal muscle show that SIRT1 is dispensable for the expression of mitochondrial genes, fiber type composition in sedentary animals, changes in fiber composition after voluntary exercise, and atrophy, suggesting that redundant mechanisms regulate these pathways in skeletal muscle. However, we did find that loss of muscle SIRT1 resulted in a modest increase in blood glucose levels and less endurance of mice to treadmill exercise.
Importantly, knocking out SIRT1 in muscle was shown to affect the response to calorie restriction , a condition known to increase sirtuin protein levels and activity in muscle , . Thus the properties of SIRT1 transgenic mice may mimic calorie-restricted mice, and are consistent with the fact that they display an increase in mitochondrial biogenesis and of slow-twitch oxidative fibers. Most dramatically, our transgenic mice are protected against tissue degeneration in a model of DMD, as detailed below.
DMD is a debilitating disease affecting 1 in 3,500 boys worldwide resulting in muscle degeneration and death. To date, effective pharmacological treatment for DMD is not available. In this study we showed that increased levels of SIRT1 in skeletal muscle of the mouse model of DMD, mdx, ameliorates the disease phenotype and improves muscle physiology and function. It was previously shown that the transcriptional coactivator PGC-1α improves the disease phenotype by regulating the NMJ genes, inducing utrophin expression, and increasing the percentage of oxidative fibers , , which are more resistant to degeneration. SIRT1 muscle-specific overexpressing mice phenocopy the PGC-1α transgenic mouse: they express high levels of markers of slow-twitch myofibers, high levels of utrophin, and high levels of NMJ genes. Importantly, SIRT1 reverses the phenotype of the mdx mouse as evidenced by intact muscles, less creatine kinase activity in the blood, and better performance in treadmill exercise. Interestingly, we observed that the expression levels of NAD+ biosynthetic enzymes and NAD+ concentration are reduced in dystrophic muscle, suggesting that the activity of NAD+-dependent enzymes, such as SIRT1, is downregulated. The reduction in NAD+ levels in SIRT1 Tg mice likely reflects increased SIRT1 activity (which degrades NAD+) in these mice, and is consistent with the suppression of phenotypes in mdx SIRT1 overexpressing mice.
Since SIRT1 can be activated by small molecules , our results offer promise for pharmacological interventions that can activate SIRT1 and alleviate the dystrophic phenotype in patients. In support of this notion, a recently published paper showed that resveratrol, a first generation SIRT1 activator , improved some of the dystrophic phenotypes such as myofiber loss and fibrosis but did not have any effects on the high levels of CK activity and therefore muscle injuries . It will be important to test newer, more potent SIRT1 activators in mdx mice. Interestingly, another study in a zebrafish model of muscular dystrophy showed that treatment with NAD+ or precursors improved the dystrophic phenotype . Our data, along with these observations, suggest that treatment with potent and specific activators of SIRT1 can offer new therapeutic approaches for treating muscular dystrophies, and perhaps sarcopenia.
Materials and Methods
All animal procedures were performed according to Massachusetts Institute of Technology Committee on Animal Care. Mice were fed standard rodent chow diet and housed in a facility with 12 hr light and dark cycles. To generate muscle-specific transgenic mice (TG), the mouse SIRT1 cDNA was cloned between the 4.8 kb promoter of muscle creatine kinase (MCK)  and the human growth hormone (hGH) polyadenyltion sequence. Mouse oocytes of the C57BL/6J genetic background were injected with this construct by the MIT transgenic facility. The SIRT1 muscle-specific knockout mice (MckKO) were generated by crossing mice being homozygous for the floxed SIRT1 allele  with mice expressing the cre recombinase under the control of MCK promoter . Both strains were in C57BL/6J genetic background. The mdx mice (C57BL/10ScSn-Dmdmdx/J)  were purchased from the Jackson Laboratories, and were crossed to SIRT1 Tg or MckKO mice to generate mdx;TG and mdx;MckKO mice. To control for variations in the genetic backgrounds all experiments were performed comparing siblings of same gender (either mdx vs. mdx;Tg or mdx;F/F vs. mdx;cre;F/F).
The treadmill exercise was performed using a motor-driven treadmill (Columbus Instruments). The running protocol used for Tg and MckKO mice, following 3 days of acclimatization at 15 m/min, was 5 min at 13 m/min (warm up) and then the speed was increased 1 m/min every minute up to 18 m/min and kept constant for 30 min. After 30 min, the speed was increased 1 m/min. The mice were considered exhausted and removed when they sat on the shocker for more than 20 sec. The running protocol we used for mdx mice was milder to allow the mice to run. Following 3 days of acclimatization at 8 m/min for 10 min, the mice ran at 4 m/min for 5 min (warm up) and the speed was increased 1 m/min every min up to 9 m/min. The mice were considered exhausted when they sat for more than 20 sec.
Plasma insulin levels were measured after overnight fasting using ELISA kit (Millipore). Blood glucose levels were measured using OneTouch strips and glucometer. To measure serum creatine kinase (CK) levels, the blood was collected in heparinized tubes, serum was isolated, and CK activity was assayed using the DiscretPak Creatinine Kinase Reagent Kit (Catachem) according to manufacturer's protocol.
Histological analyses and Evans Blue staining
Gastrocnemius muscle was fixed with formaldehyde, paraffin-embedded, cross-sectioned and stained with hematoxylin and eosin following standard procedures. The slides were analyzed using standard light microscopy. The fibers' sizes (25–30/image) were counted in 10 randomly chosen images/mouse (250–300 fibers/mouse) using ImageJ software (NIH). For SDH, COX, and ATPase stainings, gastrocnemius/soleus muscles were snap frozen in isopentane/liquid N2, cryo-sectioned, and stained for enzymatic activities using standard procedures. The stained fibers were counted and their percentage of total number of fibers was calculated (150–200 total fibers/image, 5 images/mouse, 3 mice/genotype). Evans Blue dye (1% solution) was injected intraperitoneally (1% volume/gr of body weight), and the mice were euthanized 16 hr later. Gastrocnemius muscle was dissected and embedded in OCT compound, frozen, and cross-sectioned. The slides were also stained with DAPI to visualize nuclei. Evans Blue and DAPI staining were analyzed by fluorescence microscopy. The area of Evans Blue stained fibers was counted in 10 randomly chosen images/mouse by ImageJ and the percentage of total area was calculated.
RNA, mitochondrial DNA, protein analyses, NAD+ measurements
RNA was isolated from gastrocnemius muscle using Trizol (Invitrogen) and further purified using RNeasy mini columns (Qiagen). Quantitative PCR analysis was performed on a LightCycler 480II (Roche) using iQ SYBR Green Supermix (Biorad). For mitochondrial DNA quantitation, mitochondrial and genomic DNA was isolated from gastrocnemius muscle after Proteinase K and RNAse A digestion followed by phenol-chloroform extraction. Quantitative PCR analyses were performed using mitochondrial and genomic specific primers.
Skeletal muscle protein homogenates were prepared following standard procedures. The antibodies used were against SIRT1 N-term (Millipore, #07-131), actin (Chemicon, MAB1501), AMPKα (Cell Signaling, #2603), phospho-AMPKα (Cell Signaling, #2531), p70 S6 Kinase (Cell Signaling, #2708), phospho-p70 S6 Kinase (Cell Signaling, #9205), Gapdh (Sigma, G9545), PGC1 (Santa Cruz, sc-13067), acetylated lysine (ImmuneChem, ICP0380). The immunoprecipitation was performed using the Pierce Direct-IP Kit (Thermo Scientific) according to manufacturer's instructions.
NAD+ was measured in freshly isolated gastrocnemius muscle using EnzyChrom kit from BioAssay Systems following the manufacturer's protocol.
SIRT1 transgenic lines. (A) Relative protein levels of SIRT1 in gastrocnemius muscle of WT and Tg-4140 line quantified by Image J (n = 4). SIRT1 protein is expressed at approximately ∼100 fold in Tg-4140 muscle compared to WT. (B) Western blot in tissue protein homogenates prepared from WT and SIRT1 transgenic lines 4311 and 4145. (He: heart, Qu: Quadriceps, Ga: gastrocnemius, So: soleus, L: liver.) (C) Gastrocnemius muscle weight (from one hindlimb) and muscle/body weight ratio of Tg-4311 and Tg-4145 mice at 8–10 weeks of age (n = 4–6). (D–G) Relative mRNA levels of cytochrome C, TFAM, PGC-1α, and UCP3 in gastrocnemius muscle of WT, SIRT1 Tg-4311, and Tg-4145. Data are expressed as mean +/− s.e.m. *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.
SIRT1 overexpression drives fast-to-slow fiber type switch. (A) Quantitation of fibers stained for SDH activity in WT and Tg-4140 muscle shown in Figure 3C (n = 3 animals, 500–1000 fibers counted/animal). (B) Quantitation of fibers stained for COX activity in WT and Tg-4140 muscle shown in Figure 3D (n = 3 animals, 500–1000 total fibers counted/animal). (C) Representative myosin ATPase activity staining at indicated pH of cross-sections of gastrocnemius muscle of WT and Tg-4140 mice (10× magnification). Type I fibers are stained light in pH 10.2, and dark in pH 4.31. Type II fibers are stained dark in pH 10.2 and light in pH 4.31 (n = 3 animals, 500–1000 fibers counted/animal).
Loss of SIRT1 from skeletal muscle does not affect the expression of atrophy genes under basal or atrophy-inducing conditions. (A) Relative mRNA levels of MAFBx and MuRF-1 atrophy genes in gastrocnemius muscle of WT and MckKO mice fed or fasted for 24 hrs (n = 3–5). (B) Relative mRNA levels of MAFBx and MuRF-1 atrophy genes in gastrocnemius muscle of WT and MckKO mice, which underwent mock surgery (control) or were denervated for 3 days (n = 3–5). Data are expressed as mean +/− s.e.m.
SIRT1 Tg-4145 exhibits protective signs against DMD, and SIRT1 loss from skeletal muscle does not affect the muscular dystrophic phenotype of mdx mouse. (A) Relative mRNA levels of utrophin in gastrocnemius muscle of Tg-4145 line (8–10 weeks old, n = 3) (B) Serum creatine kinase activity in mdx, mdx Tg-4145 mice (14 weeks old, n = 6–10). (C) Serum creatine kinase activity in mdx and mdx;MckKO mice (8–10 weeks old, n = 10). (D) Distance run in treadmill exercise by mdx and mdx;MckKO mice (12–14 weeks old, n = 10–12). (E) Relative mRNA levels of acetylcholine receptor (AchR) subunits α, δ, ε, Musk, Erbb, and Raspn in gastrocnemius muscle from WT and MckKO mice (n = 3–5), and (F) mdx and mdx;MckKO mice (n = 3–5). Data are expressed as mean +/− s.e.m. *p<0.05, n.s: non-significant.
We thank Guarente lab members for helpful discussions, and K. Boulias for comments on the manuscript. We also thank MIT Transgenic Facility for assistance with the generation of transgenic mice and the Histology Facility at the Koch Institute at MIT for H&E stainings and cryo-sections.
Conceived and designed the experiments: AC LG. Performed the experiments: AC MI ASN JK. Analyzed the data: AC LG. Wrote the paper: AC LG.
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