The Fragile X-Related 1 gene (FXR1) is a paralog of the Fragile X Mental Retardation 1 gene (FMR1), whose absence causes the Fragile X syndrome, the most common form of inherited intellectual disability. FXR1P plays an important role in normal muscle development, and its absence causes muscular abnormalities in mice, frog, and zebrafish. Seven alternatively spliced FXR1 transcripts have been identified and two of them are skeletal muscle-specific. A reduction of these isoforms is found in myoblasts from Facio-Scapulo Humeral Dystrophy (FSHD) patients. FXR1P is an RNA–binding protein involved in translational control; however, so far, no mRNA target of FXR1P has been linked to the drastic muscular phenotypes caused by its absence. In this study, gene expression profiling of C2C12 myoblasts reveals that transcripts involved in cell cycle and muscular development pathways are modulated by Fxr1-depletion. We observed an increase of p21—a regulator of cell-cycle progression—in Fxr1-knocked-down mouse C2C12 and FSHD human myoblasts. Rescue of this molecular phenotype is possible by re-expressing human FXR1P in Fxr1-depleted C2C12 cells. FXR1P muscle-specific isoforms bind p21 mRNA via direct interaction with a conserved G-quadruplex located in its 3′ untranslated region. The FXR1P/G-quadruplex complex reduces the half-life of p21 mRNA. In the absence of FXR1P, the upregulation of p21 mRNA determines the elevated level of its protein product that affects cell-cycle progression inducing a premature cell-cycle exit and generating a pool of cells blocked at G0. Our study describes a novel role of FXR1P that has crucial implications for the understanding of its role during myogenesis and muscle development, since we show here that in its absence a reduced number of myoblasts will be available for muscle formation/regeneration, shedding new light into the pathophysiology of FSHD.
Muscle development is a complex process controlled by the timely expression of genes encoding crucial regulators of the muscle cell precursors called myoblasts. We know from previous studies that inactivation of the Fragile X related 1 (FXR1) gene in various animal models (mouse, frog, and zebrafish) causes muscular and cardiac abnormalities. Also, FXR1P is reduced in a human myopathy called Fascio-Scapulo Humeral Dystrophy (FSHD), suggesting its critical role in muscle that findings presented in this study contribute to elucidating. Cell-cycle arrest is a prerequisite to differentiation of myoblasts into mature myotubes, which will form the muscle. One key regulator is the p21/Cdkn1a/Cip1/Waf1 protein, which commands myoblasts to stop proliferating, and this action is particularly important during muscle regeneration. In this study, we have identified FXR1P as a novel regulator of p21 expression. We show that FXR1P absence in mouse myoblasts and FSHD-derived myopathic myoblasts increases abnormally the levels of p21, causing a premature cell cycle exit of myoblasts. Our study predicts that FXR1P absence leads to a reduced number of myoblasts available for muscle formation and regeneration. This explains the drastic effects of FXR1 inactivation on muscle and brings a better understanding of the molecular/cellular bases of FSHD.
Citation: Davidovic L, Durand N, Khalfallah O, Tabet R, Barbry P, Mari B, et al. (2013) A Novel Role for the RNA–Binding Protein FXR1P in Myoblasts Cell-Cycle Progression by Modulating p21/Cdkn1a/Cip1/Waf1 mRNA Stability. PLoS Genet 9(3): e1003367. https://doi.org/10.1371/journal.pgen.1003367
Editor: Gregory A. Cox, The Jackson Laboratory, United States of America
Received: August 21, 2012; Accepted: January 21, 2013; Published: March 21, 2013
Copyright: © 2013 Davidovic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: LD was funded by CNRS, the FRAXA Research Foundation 2010-12, and the Marie Curie European Community Program (FP6 MEIF-CT-2006-41096 and FP7-PEOPLE-ERG-2008-239290). BB was funded by CNRS, LIA “NEOGENEX,” INSERM, Agence Nationale de la Recherche (ANR) grant ANR-09-RARE-02-05, and by two French charities: Fondation Recherche Médicale call TEAM FRM 2009 and AFM (Association Française contre les Myopathies) Call MNMP2010 grant NR 13536. BB and LD were supported by Conseil Général Region PACA. OK was supported by a “Ville de Nice” post-doctoral fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The Fragile X-Related 1 (FXR1) gene belongs to a small gene family that includes the Fragile X Mental Retardation 1 (FMR1) and Fragile X-Related 2 (FXR2) genes (reviewed in ). Human FMR1 is located on chromosome Xq27.3  and inactivation of FMR1 expression leads to the Fragile X syndrome in human, the first cause of inherited mental retardation . FXR1 and FXR2 are autosomal genes, respectively mapping at 3q28 and 17p13.1 , . The FXR1 gene is highly expressed in muscle and its pre-mRNA is known to undergo extensive alternative splicing, which generates distinct FXR1 mRNA variants that produce FXR1P isoforms with divergent C-terminal regions , . Four isoforms ranging from 70 to 80 KDa (Isoa, Isob, Isoc, Isod) are ubiquitously expressed, including in murine ,  and human myoblasts . Myoblasts also express long muscle-specific FXR1 mRNA variants, termed Isoe and Isof, which are massively induced upon muscular differentiation , , , . Importantly, these muscle-specific mRNA variants of FXR1 are the only expressed in adult muscle , , , , . Defects in FXR1 gene muscular pattern of expression have been observed in patients affected by Facio-Scapulo Humeral Distrophy (FSHD), the most prevalent muscular dystrophy affecting adults and children . Similar defects were observed in a mouse model of myotonic dystrophy (DM1, ). As a result, the long isoforms FXR1P Isoe and Isof of 82–84 kDa are depleted in myopathic muscle. Consistent with these altered expression pattern of FXR1 in myopathic patients, Fxr1-knockout mouse die shortly after birth most likely due to an abnormal development of cardiac and respiratory muscles . A mouse model with reduced levels of Fxr1 expression has also been generated, and displays reduced limb musculature and a shorter life span of about 18 weeks . Moreover, during Xenopus embryogenesis, complete or partial inactivation of xFxr1 disrupts somitic myotomal cell rotation and segmentation, impeding normal myogenesis . Finally, depletion of zFxr1p during early development of the zebrafish leads to cardiomyopathy and muscular distrophy . All these data point out an evolutionarily conserved role for FXR1P in myogenesis.
FXR1P contains two KH domains and one RGG box that are characteristic motifs in RNA-binding proteins , . In addition, FXR1P harbours nuclear localization and export signals (NLS and NES) enabling nucleocytoplasmic shuttling , . In most cell types and tissues studied, FXR1P isoforms are associated to messenger ribonucleoparticles (mRNPs) present on polyribosomes, suggesting a consensus role in translation regulation for FXR1P . However, it was reported that, in undifferentiated myoblasts, FXR1P long isoforms Isoe and Isof are not detected on polyribosomes, suggesting a role other than translation regulation for these isoforms at this stage , . Very few specific target mRNAs for FXR1P have been identified so far, and even more scarcely in the context of myogenesis. First, two independent studies reported that the shortest isoform of FXR1P, Isoa, binds the AU-rich element (ARE) present in the 3′UTR of proinflammatory cytokine tumor necrosis factor (TNFα) mRNA , . In this context, FXR1P associates with AGO2 on TNFα−ARE to modulate its translation . Second, we have previously shown the ability of FXR1P Isoe, its long muscle-specific isoform, to interact specifically and with high affinity with the G-quadruplex RNA structure in vitro . However, no mRNA target of FXR1P bearing a G-quadruplex has been identified yet in vivo. Finally, one study reports the presence of Desmoplakin and Talin2 mRNAs in FXR1P-mRNP complexes and subsequent disturbance of the expression of the encoded proteins in Fxr1-KO heart extracts . However, neither the binding motif/sequence recognized by FXR1P on these mRNAs nor the exact functional significance of these interactions have been explored.
To gain further insights into the muscular roles of FXR1P and the pathways perturbed in its absence, we performed a large-scale microarray analysis of the C2C12 myoblastic cell line inactivated for Fxr1. This analysis revealed that Fxr1-depletion lead to premature cell cycle exit of myoblasts. We link this to a robust increase in the levels of the cyclin-dependant inhibitor p21/Cdkn1a/Cip1/Waf1, that is also observed in FSHD-derived myoblasts. In this study, we further explore the role played by the direct interaction of FXR1P with p21 mRNA in the post-transcriptional control of p21 levels.
Inactivation of Fxr1 in C2C12 myoblasts selectively affects the expression of a range of genes associated with cell-cycle regulation during muscle development
To understand the functional role of FXR1P in myoblasts, we used as a cellular model the C2C12 myoblastic cell line. This murine cell line enables to reproduce myogenesis in vitro  and expresses all the myogenic factors as well as FXR1P , . In this model, we inactivated the expression of all FXR1P isoforms by transient transfection of siRNAs targeting exon 14, a constitutive exon present in all Fxr1 mRNAs . As shown in Figure 1A, quantitative RT-PCR performed on C2C12 cells transfected with siFxr1 siRNAs reveals a significant reduction in Fxr1 mRNA as compared to siControl-transfected cells (13.45%±3.4% residual expression, Figure 1A). Knockdown of all isoforms of FXR1P was obtained by siFxr1 transfection, as shown by western-blot analysis using the 3FX antibody (Figure 1B, ). Note that the levels of FXR2P, the close homologue of FXR1P, also recognized by 3FX antibody, remain unaffected, confirming the specificity of the knockdown strategy (asterisk, Figure 1B). In siFxr1-transfected myoblasts, the decrease in epifluorescence signal after FXR1P-immunolabeling as compared to siControl-transfected cells confirms the efficiency of the knockdown (Figure 1C). The knockdown appears to homogenously affect all the cells since the signal is uniformly decreased. Note that in C2C12 cells, FXR1P immunoreactivity is mainly cytoplasmic, however, signal is also detected in the nucleus (Figure 1C). Indeed, we confirmed the partial nuclear localization of FXR1P in myoblasts by confocal microscopy (Figure 1D), as described previously for the long isoforms of FXR1P in C2C12 myoblasts  and in human myoblasts .
(A) Quantitative RT-PCR reveals a strong reduction of Fxr1 mRNA in C2C12 cells transfected with siRNA against Fxr1 compared to siControl-transfected cells. (B) Western-blot analysis of untransfected (UT) and siFxr1-transfected cells (siFxr1) revealed with the antibody #3FX recognizing all isoforms of FXR1P reveals a strong depletion of all isoforms of FXR1P (short, medium and long) compared to control (siCtl), while the levels of FXR2P protein (asterisk, *) remain unchanged. β-tubulin (β-tub) signal is used to verify equal loading of lanes. (C) Immunofluorescence analysis of FXR1P (red) subcellular distribution in siControl and siFxr1-transfected cells, using polyclonal #830 anti-FXR1P antibodies. Nuclei were counterstained with DAPI (blue) and merge images are shown in the right panel. The same exposure time was used for both image captures and reveal a strong depletion in FXR1P signal in siFxr1-transfected cells compared to control (siControl). Scale bar: 15 µm. (D) Confocal micrographs of C2C12 cells immunostained for FXR1P reveal a nucleocytoplasmic distribution of FXR1P. Please note the nuclear dot-like structures containing FXR1P. Slice depth: 1 µm, scale bar: 15 µm. (E) Volcano plot showing the distribution of differentially expressed transcripts between C2C12 cells transfected with siRNA against Fxr1 versus siControl-transfected cells. Log of the fold of change (LogFC) is plotted against the B-statistic value for each transcript. A subset of 9 transcripts selected for further validation by Quantitative-RT PCR (Fxr1, Cdk15, Sema7a, Mctp2, Asgr1, Hgf, p21, Dusp6 and Lbh) are highlighted. Significantly down- and up-regulated genes are shown in green and red, respectively. (F) Quantitative-RT PCR analysis of a subset of mRNAs confirm that Sema7a, Mctp2, Asgr1, p21, Hgf, Dusp6, Lbh, MyoD and Myog are significantly upregulated in Fxr1-depleted C2C12 myoblast, while Cdk15 is downregulated, confirming the microarray analysis. Data are presented as means ± SEM of n = 4 experiments.
To determine the impact of the inactivation of Fxr1 on gene expression in myoblasts, total RNA was extracted from siControl and siFxr1-transfected C2C12 myoblasts and simultaneously analysed using whole genome mouse microarrays. Among the genes showing measurable differential levels of expression, a significant change was observed for 105 transcripts (32 down- and 73 up-regulated) of which 79 were annotated in the RefSeq database (Figure 1E and Table S1). As expected, Fxr1 mRNA appears among the most significantly down-regulated in siFxr1-transfected cells (Figure 1E and Table S1). To confirm the observed dysregulation of a subset of mRNAs in Fxr1-knockdown C2C12 myoblasts, we performed quantitative RT-PCR analysis (Figure 1F). Interestingly, in Fxr1-depleted myoblasts, we were able to confirm by quantitative RT-PCR a significant upregulation of mRNAs encoding: Semaphorin 7a (Sema7a), the Ca2+-binding multiple C2 domains transmembrane protein 2 (Mctp2), asialoglycoprotein receptor 1 (Asgr1), the cyclin-dependant kinase inhibitor p21 (p21/Cdkn1a/Waf1/Cip1), Hepatocyte growth factor (Hgf), Dual specific phosphatase (Dusp6) and finally Limb-bud and heart protein (Lbh, Figure 1E). Conversely, we confirmed a significant down-regulation of Cdk15 mRNA encoding the cyclin-dependent kinase 15. Finally, the mRNAs encoding the myoregulatory factors MyoD and Myogenin for which no mRNA variations were detected by microarray analysis remained unaffected (Figure 1F). These analyses were further repeated on C2C12 cells inactivated for Fxr1 by transfection of a different siRNA (siFxr1#2) targeting Fxr1 exon 6, another constitutive exon of Fxr1 present in all its variants . This second siRNA leads to a 37% residual expression of Fxr1 mRNA (Figure S1A) and reduces all FXR1P isoforms (Figure S1B) as compared to siControl. In addition, siFxr1#2-mediated knockdown of Fxr1 efficiently modulated the previously studied subset of mRNAs to induce variations similar to the one observed with the first siRNA against Fxr1 (Figure S1C). Importantly, this cross-analysis using two siRNAs targeting distinct regions of Fxr1 mRNA exclude the fact that the observed variations could derive from off-target effects of the siRNAs.
To gain insights into the pathways perturbed by Fxr1 depletion, we performed an analysis of the biological functions or processes selectively enriched among the altered transcripts, using the Ingenuity Pathway Analysis (IPA) software (Table S2). Interestingly, Fxr1 knockdown in C2C12 myoblasts significantly affected the functional categories ‘cell cycle’ (Table S2), ‘skeletal and muscular system development and function’ and ‘skeletal and muscular disorders’ (Table S2). Importantly enough, a subset of mRNAs perturbed in siFxr1-knockdown myoblasts compared to control repeatedly appeared determinant for the definition of the affected functional categories: the cyclin-dependent kinase (Cdk15), the cyclin-dependent kinase inhibitor (p21/Cdkn1a/Cip1/Waf1) and the Hepatocyte growth factor (Hgf).
Fxr1-depletion in myoblasts leads to premature exit of cell cycle
One of the most recurrent terms in IPA analysis of dysregulated mRNA upon Fxr1 depletion were ‘cell cycle progression’, ‘arrest in G0/G1’, ‘proliferation’ and also ‘cell viability’ (Table S2). This prompted us to analyse myoblasts' viability and proliferation abilities upon Fxr1-depletion. Fluorescence-Activated Cell Sorting (FACS) analysis of the DNA intercalant Propidium Iodide (PI) incorporation on living cells allowed us to detect no changes in the overall viability of Fxr1-knockdown (92.5% viability) compared to control (90.53% viability) C2C12 cells (Figure 2A). To assess the proliferation ability of Fxr1-depleted myoblasts, we conducted tetrazole MTT proliferation assays. Interestingly, after 48 hours in culture, siFxr1-transfected C2C12 cells exhibit a significant 15% decrease in MTT reductase activity as compared to control (Figure 2B). This suggests that Fxr1 depletion may induce alterations of myoblasts cell cycle. We therefore further analysed the distribution in the various cell cycle phases of siFxr1- or siControl transfected myoblasts. The DNA content of the cells was assessed by FACS-measurement of the amount of PI incorporated in cells. Surprisingly, in a normal asynchronous cell population, we did not observe any significant change in the cell cycle phases distribution of the C2C12 cells transfected with siFxr1 or siControl, in normal growth conditions (Figure 2C).
(A) PI incorporation in living siFxr1- or siControl-transfected cultures and subsequent FACS analysis was performed to show that viability of the culture is not affected by Fxr1-depletion. (B) MTT colorimetric assay show that the proliferation abilities of C2C12 cells are significantly impaired by Fxr1-depletion. (C) FACS analysis of the Propidium Iodure-stained DNA content of C2C12 cells transfected with siControl or siFxr1. Cells were analysed in asynchronous conditions or following synchronisation treatment for 8 hrs with the cell cycle blocker mimosine (late G1) followed by 16 hrs release in normal growth medium (D). In asynchronous conditions, cell cycle distribution is similar in siControl or siFxr1 transfected cells. Synchronisation of cells allows detecting significant differences in the distribution of the cells in the various cell cycle absence of FXR1P: increase in the G0/G1 proportion and decrease in the G2/M. Data are presented as means ± SEM of n = 4 experiments, with FACS analysis of a minimal cell population of 15,000 for each condition and each experiment. The asterisk (*) indicates p<0.05 of a Mann & Whitney test.
To highlight specific defects in cell cycle, we synchronized siFxr1- and siControl-transfected myoblasts by treatment with the cell cycle blocker mimosine, that arrests cell cycle progression at the G1/S phase border . Since the effects of this cell cycle blocker are fully reversible, we then allowed the synchronized cells to reenter cell cycle by incubating them in normal growth medium for 16 hrs before FACS analysis. In these conditions, we did observe a significant 27.6% increase in the number of cells in the G0/G1 phase in Fxr1-knockdown myoblasts, as compared to control. This increase in the G0/G1 population is accompanied by a 51.9% decrease in the number of cells in the G2/M phase. Importantly, no differences were observed in the proportion of cells in the Sub-G1 phase - corresponding to cellular debris with a lower DNA content liberated by apoptotic cells - in asynchronous cells (Figure 2A) and after release from cell cycle blocker (Figure 2D). These data indicate that FXR1P depletion in myoblasts does not lead to cell viability defects but rather causes a blockade and accumulation of cells in the G0/G1 phase to the detriment of mitosis.
Thus, to determine whether the cells were blocked in G0 or G1, we performed immunolabeling of C2C12 cells in normal growth conditions and quantified the number of DAPI-positive nuclei and the amount of cells positive for the proliferation marker Ki67 (Figure 3). We observed that the number of nuclei in cultures of siFxr1-transfected myoblasts is decreased by 26%, suggesting that Fxr1 depletion limits the proliferating abilities of myoblasts (Figure 3B). Quantification of cells expressing Ki67 enabled us to detect that siRNA-meditated depletion in Fxr1 leads to a subtle, but significant 10% decrease in the number of Ki67-positive cells compared to control (Figure 3C). Since Ki67 is expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent from quiescent cells (G0) , the unlabeled cells most likely represent resting cells blocked in G0.
(A) Immunofluorescence analysis of C2C12 cells transfected with siControl or siFxr1. Nuclei are stained with DAPI (blue) and cells expressing the proliferation marker Ki67 are labelled with FITC antibody (green). Scale bar: 75 µm. (B) Quantification of the number of DAPI-stained nuclei. (C) Quantification of the number of Ki67-positive cells over total number of nuclei quantified in (B). Quantification was performed using a macro developed with the ImageJ software. Data presented are mean of n = 4 experiments with analysis of 10 optical fields for each condition and each experiment. The asterisk (*) indicates p<0.05 for the Student T-test.
The absence of FXR1P in C2C12 cells and in FSHD patients-derived myoblasts affects the levels of endogenous p21 mRNA and protein
The premature cell cycle arrest we observed in Fxr1-depleted myoblasts prompted us to examine the subset of deregulated mRNAs identified by microarray analysis in order to identify candidates for regulation by FXR1P that could contribute to explain this phenotype. The most promising mRNA candidate appeared to encode the ubiquitous cyclin-dependent kinase inhibitor (CDKI) p21 –also known as Cdkn1a/Cip1/Waf1- that belongs to the Cip/Kip family of CDKI. In myoblasts, p21 is known to block cell cycle progression to trigger cell-cycle exit, a prerequisite to muscular differentiation , , .
In Fxr1-depleted myoblasts, we found that p21 mRNA level is significantly increased by microarray analysis (Figure 1E, Table S1) and confirmed a 1.76-fold upregulation of the transcript by quantitative-RT PCR in these Fxr1 loss-of-function experiments (cf Figure 1F). This upregulation of p21 mRNA level in Fxr1-depleted myobasts was further confirmed using a second siRNA targeting Fxr1 (Figure S1). We had previously shown that the muscle-specific long isoforms of FXR1P, notably Isoe, are depleted in myoblasts derived from Fascio-ScapuloHumeral Distrophy (FSHD) patients and had hypothesized that this could induce deregulation of mRNA targets specific to this isoform FXR1P Isoe . To test this hypothesis on this new potential mRNA target of FXR1P, we assessed the status of human P21 in the same samples used in our previous study. Interestingly enough, P21 mRNA levels are significantly increased in FSHD patients by a 1.8 factor (Figure 4A).
(A) Quantitative RT-PCR reveals a significant increase of P21 mRNA in FSHD myoblats relative to control individuals. Data are presented as means ± SEM of n = 3 individuals/group. (B) Representative western-blot of p21 protein levels in siControl (siC) or siFxr1 (siFx)-transfected C2C12 cells. Densitometric quantification of western-blots reveal that depletion of FXR1P by siRNA transfection (siFxr1) leads to a significant increase of p21 protein levels relative to siControl-transfected cells. Data are presented as means ± SEM of n = 4 experiments. (C) Representative western-blot of P21 protein levels in FSHD patients and control individuals. Densitometric quantification of western-blots reveals that muscle biopsies of FSHD patients display a significant increase of P21 protein relative to controls. Data are presented as means ± SEM of n = 3 individuals/group. The asterisks * and ** indicate respectively p<0.05 and p<0.01 of the Mann & Whitney test.
We then sought to verify whether this increase in p21 at the mRNA level was translated at the protein level by western-blotting (WB) analysis. Quantification of WB of siFxr1-transfected C2C12 using the ImageJ software revealed a 1.92 fold increase in p21 protein levels (Figure 4B). Concomitantly, we observed by western-blotting that the levels of P21 protein are increased in FSHD myoblasts compared to control by a 1.66 factor (Figure 4C). These data indicate that depletion of FXR1P and particularly of its long muscle-specific isoforms increases p21 mRNA and correlatively increase the levels of p21 protein both in murine and human myoblasts.
To assess the specificity and the direct nature of the effects we observed on p21 mRNA levels by FXR1P loss of function experiments, we first used a gain-of-function approach. For these experiments, we used FXR1P long isoform Isoe since its depletion in FSHD myoblasts recapitulates the effects on p21 mRNA levels of a knockdown of all FXR1P isoforms in C2C12 cells (cf Figure 4). Interestingly, in contrast to Fxr1 loss-of-function in C2C12 myoblasts, over expression of FXR1P Isoe lead to a 19,1% significant decrease in endogenous p21 mRNA levels as compared to transfection with empty vector (Figure 5A). This ascertains the fact that the effects we observe on p21 mRNA levels are directly related to the levels of FXR1P present in the cell. Secondly, we performed rescue experiments using a pTL1 plasmid bearing FXR1 Isoe cDNA in which we generated by site-directed mutagenesis 4 mismatches to avoid recognition of the transgene by siFxr1 (Figure 5B). This strategy enabled to efficiently re-express FXR1P Isoe in Fxr1-knocked down myoblasts (Figure 5C). Rescue of the expression of FXR1P Isoe lead to a significant reduction in p21 mRNA levels as compared to unrescued myoblasts. The rescue with FXR1P Isoe is total since the levels of p21 mRNA in rescued cells are restored to control levels. Of notice, similar results were obtained using another mutant plasmid of pTL1.Isoe (data not shown), confirming the efficiency of the rescue strategy.
(A) Western-blot analysis (upper panel) of C2C12 cells transfected with empty pTL1 vector or pTL1.FXR1 Isoe (pTL1.Isoe) construct indicate a strong expression of FXR1P long isoform Isoe in transfected myoblasts. Quantitative RT-PCR (lower panel) reveals a significant decrease of p21 mRNA levels in C2C12 myoblasts overexpressing FXR1 Isoe, as compared to control. Data are presented as means ± SEM of n = 3 independent experiments. (B) Western-blot analysis (upper panel) of C2C12 cells transfected with control siRNA (siC) or siFxr1 (siFx) and empty pTL1 vector or a mutated version of pTL1.FXR1 Isoe (pTL1.Isoe*) bearing 4 mismatches in siFxr1 recognition sequence indicate a reexpression of FXR1P long isoform Isoe in Fxr1-depleted transfected myoblasts. In the western blot FXR2P is indicated by (*) Quantitative RT-PCR (lower panel) reveals a significant increase of p21 mRNA levels in C2C12 myoblasts transfected with siFxr1 (siFx) and the empty vector (pTL1), as compared to control. This increase is restored to normal levels when FXR1P Isoe expression is rescued by transfection of pTL1.FXR1 Isoe. Data are presented as means ± SEM of n = 3 independent experiments. The asterisks * indicate p<0.05 of the Wilcoxon paired test, ns indicates non significance.
These data confirm the specificity of our approach and suggests that p21 mRNA may be a target of FXR1P in C2C12 murine myoblasts and in human myoblasts, either directly by RNA-protein physical interaction, or indirectly by modulating a pathway involved in p21 levels controls.
p21 mRNA is a novel mRNA target of FXR1P, both in vitro and in vivo
Murine p21 mRNA is 1910 nts long (GenBank Accession number: GI 161760647), with a very short 5′UTR of less than 100 nts, a 480 nts coding sequence and a 1329 nts long 3′UTR where lie most of the regulatory elements for the stability of this mRNA (Figure 6A). Notably, the ARE located at position 86–103 nts on the 3′UTR is bound by the RNA-binding protein HuR to regulate the stability of the mRNA during muscle differentiation . Given the ability of FXR1P Isoa to bind ARE sequences , , we hypothesized that the ARE present in p21 mRNA could be the binding site of FXR1P.
(A) Scheme of the various portions of p21 mRNA 3′ UTR (α, β and γ) used for in vitro binding assays. Note that the α fragment contains a characterized ARE motif. (B) Nitrocellulose filter binding assays to determine the portion of p21 mRNA bound by FXR1P. Radiolabeled mRNA probes were incubated with increasing concentrations of recombinant FXR1P Isoe protein, the amount of radioactive probes recovered on filters after binding reaction is then plotted against the concentration of proteins. The portion of FMR1 mRNA called N19 (known to be bound by FXR1P) and its truncated version (N19Δ35) were used as controls. This reveals that the distal portion of p21 3′UTR (γ fragment) and N19 are selectively bound by FXR1P. Both the α and β fragments from the 3′UTR of p21 remain at background levels comparable to N19Δ35 binding to FXR1P. (C) Western-blot analysis of UV-crosslinking and immunoprecipitation (CLIP) assay performed on C2C12 lysates using polyclonal antibodies raised against the C-terminus of FXR1P (#830) and control rabbit IgG (R). Input lysates (lanes 1 & 2, Input, 1/50th), immunoprecipitates (lanes 3 & 4, IP, 1/5th) and post-immunoprecipitation supernatants (lanes 5 & 6, post, 1/50th) were probed for FXR1P using the 3FX antibody. A selective enrichment in FXR1P medium and long isoforms is observed in #830 immunoprecipitate (lane 3), concomitant with a depletion in these isoforms in the post-immunoprecipitation supernatant (lane 4) as compared to corresponding controls (lane 3 & 5). (D) RT-PCR analysis of mRNAs associated with FXR1P complexes. RNA was extracted from input and immunoprecipitate fractions described in (C), and used as template for RT-PCR. RT-PCR products obtained from inputs and immunoprecipitations respectively from control with rabbit IgG (Lanes 1, 3) and immunoprecipitation of FXR1P using #830 (Lanes 2, 4) were separated and visualized by agarose gel electrophoresis. This reveals that p21 mRNA is selectively enriched in the #830 immunoprecipitates, while the mRNAs encoding the myogenic determination factors Myogenin and MyoD or the unrelated mRNA encoding β-tubulin are not recovered in any immunoprecipitates. The symbol # indicates aspecific PCR products corresponding to β-tub primers dimers. DNA molecular weight markers presented on the gels are respectively 100, 200, 300, 400, 500, 600, 800 and 1000 bp.
To test the physical interaction between FXR1P and p21 mRNA and determine the portion of the mRNA involved in the interaction, we performed in vitro filter-binding assays  using recombinant FXR1P and radiolabeled fragments of p21 mRNA 3′UTR described in Figure 5A. We chose to use FXR1P Isoe, the longest muscle-specific isoform of FXR1P for binding experiments since i) it was described to have RNA-binding properties , ii) its depletion in FSHD myoblasts recapitulates the effect on p21 mRNA levels of a complete knockdown of all FXR1P isoforms in C2C12 cells (cf Figure 4) and iii) Isoe is able to restore p21 mRNA levels to normal in Fxr1-knockdown myoblasts (cf Figure 5). As controls for interaction, we used the N19 fragment of FMR1 mRNA containing a G-quadruplex RNA structure , known to be specifically bound by FXR1P Isoe, and its truncated version N19Δ35 unable to be bound by FXR1P . As expected, FXR1P was able to recognize the G-quadruplex containing N19 fragment (Figure 6B). Surprisingly, the binding activity of FXR1P towards p21 3′UTR-α fragment (nts 1–345) that contains a well characterized ARE sequence was null, being equal to the binding activity of the negative control N19Δ35. Also, p21 3′UTR-β fragment (nts 324–868) was not recognized by FXR1P. Interestingly, the most distal portion of p21 3′UTR, termed γ fragment (nts 851–1321), was specifically bound by FXR1P. These data indicate that FXR1P Isoe does not recognize p21 mRNA via the ARE motif present in the proximal portion of the 3′UTR (α fragment), but most likely via an uncharacterized motif or sequence present in the distal portion of its 3′UTR-γ fragment.
Knowing that FXR1P interacted, at least in vitro, with p21 mRNA, we further sought to validate that this interaction occurs in vivo. To test this hypothesis, we isolated immunocomplexes containing FXR1P by performing UV-crosslinking and immunoprecipitation assays (CLIP, ). Immunoprecipitation of FXR1P mRNA complexes was carried out using the polyclonal antibody #830 against exon 16 of FXR1P present in all isoforms except the short ones ,  on C2C12 cell extracts (Figure 6C). Control CLIP was performed using non-immune rabbit IgGs. As expected, using the #3FX monoclonal antibody  against the constitutive exon 14 present in all isoforms of FXR1P, all the isoforms of FXR1P were detected in both inputs (Figure 6C, lane 1 and 2). Medium and long isoforms of FXR1P were selectively enriched in #830 immunoprecipitates (Figure 6C, Lane 4) and concomitantly depleted in #830 post-immunoprecipitation supernatant (Figure 6C, lane 6). The low amount of FXR1P small isoforms detected in the #830 immunoprecipitates most likely corresponds to the fraction of small isoforms interacting with FXR1P medium and long isoforms, since FXR1P is known to homodimerize . In contrast, FXR1P is not recovered in immunoprecipitates obtained with control rabbit IgGs (Figure 6C, lane 3) and still present in the corresponding post-immunoprecipitation supernatant (Figure 6C, lane 5), confirming the specificity of the CLIP assay performed with #830 antibodies.
RT-PCR analysis of mRNAs extracted from both inputs and immunoprecipitates was then carried out (Figure 6D). The mRNA encoding p21, β-tubulin and the myogenic factors Myogenin and MyoD are detected in the input fractions (Figure 6D, lanes 1 and 2). Interestingly, only p21 mRNA was found selectively enriched in #830 immunoprecipitates (Figure 6D, lane 4) as compared to control immunoprecipitates (Figure 6D, lane 3), while Myogenin, MyoD and β-tubulin mRNAs were undetectable. This confirms the specificity of the approach and suggests that, in the C2C12 myoblastic cell line, endogenous p21 mRNA is present in mRNA complexes containing FXR1P.
The γ fragment of p21 3′UTR recognized by FXR1P has intrinsic stabilization properties
To elucidate the functional significance of FXR1P interaction with p21 3′UTR-γ fragment, we conducted luciferase assays on C2C12 cells expressing FXR1P normally (siControl-transfected) and inactivated for Fxr1 (siFxr1-transfected). The various portions of p21 3′UTR used for binding assays were cloned in the 3′ of Renilla luciferase cDNA in a reporter system (Figure 7A). The influence of the 3′ regulatory elements on Renilla mRNA and protein levels was then assessed, in the presence and in the absence of FXR1P, and compared to control vector without regulatory elements in the 3′UTR (Figure 7B, 7C). In the presence of FXR1P or when FXR1P is knocked-down, no significant difference to control is observed in the Renilla mRNA levels, when its cDNA is fused either to the proximal α or central β fragments of p21 mRNA 3′UTR. However, the distal γfragment bound by FXR1P significantly increases Renilla mRNA levels in the presence of FXR1P (1.33-fold, Figure 7B). Intriguingly, removal of FXR1P by siRNA-mediated knockdown potentiated the mRNA stabilizing effect of the p21 3′UTR-γ fragment (1.76-fold; Figure 7B) compared to control. To assess whether variations of Renilla mRNA correlated to protein variations, we performed classical luminescence luciferase assays (Figure 7C). Interestingly, Fxr1-depletion lead to a significant increase in Renilla luciferase activity when its cDNA was either fused to the central β or distal γ fragment of p21 3′UTR (Figure 7C). However, the amplitude of variation was, again, higher when considering the γ fragment in siControl conditions (2.2-fold) or Fxr1 knockdown conditions (3.4-fold), compared to control empty vector. These data support the hypothesis that FXR1P normally destabilizes p21 mRNA via binding to a motif present in the distal γ portion of its 3′UTR.
(A) Scheme of the constructs bearing various portions of p21 mRNA 3′UTR (α, β and γ) used for luciferase assays. (B) Effect of p21 3′UTR−α, −β and −γ fragments on Renilla luciferase (Ren) mRNA levels in C2C12 cells transfected with control siRNAs (siControl) or siRNAs targeting Fxr1 (siFxr1). Quantitative RT-PCR analysis of the levels of Ren mRNA normalised to Firefly (Luc) mRNA relative to the empty construct are presented. In siControl cells, only the γ fragment significantly increased Ren mRNA levels, this effect is potentiated by Fxr1 depletion with siFxr1. In contrast, the α and β fragment have no effect on Ren mRNA levels, in the presence or absence of FXR1P. The results are presented as the means ±SEM of 4 experiments. (C) Effect of p21 3′UTR and its α, β and γ fragments on Renilla Luciferase activity in C2C12 cells transfected with control siRNAs (siControl) or siRNAs targeting Fxr1 (siFxr1). Results presented here represent the mean of the ratio of Luc-FL, Luc-α, Luc-β and Luc-γ to Luc-empty signal. In siControl cells, only the γ fragment significantly increased luciferase activity. In siFxr1 transfected cells compared to controls, the β and γ fragments increased luciferase activity, while the α fragment has no effect. However, the amplitude of variation is greater with the γ fragment and this effect is potentiated by Fxr1 depletion. Six independent experiments in triplicate for each transfection were quantified. For each transfection, Renilla was normalized to Firefly luciferase activity. RLU, relative luciferase units. (D) Fxr1-depletion increases the stability of endogenous p21 mRNA. C2C12 transfected with siControl (empty squares) or siFxr1 (black squares) were treated with the transcription inhibitor actinomycin D for 8 hrs. p21 mRNA levels were determined by quantitative RT-PCR at several time points and normalised to levels before treatment (t0). Percentage of remaining mRNA is plotted using a semi-log scale. Data presented represent the mean of n = 3 experiments. The asterisks * indicate p<0.05 of the Mann & Whitney test, while # and ## indicate respectively p<0.05 and p<0.01 of the Wilcoxon test.
To test in vivo the hypothesis of FXR1P involvement in the control of endogenous p21 mRNA stability, we treated siControl- or siFxr1-transfected C2C12 cells with the transcription inhibitor actinomycin D (ActD), and measured the decay rate of p21 mRNA by quantitative RT-PCR. Interestingly, p21 mRNA appears to cycle rapidly in control myoblasts. Linear regression on semi-log values of p21 mRNA decay rate in siControl-transfected cells, provides an estimated half-life of 2.57±0.14 hrs (Figure 7D), with only 16% mRNA remaining after 8 hrs. Conversely, upon Fxr1-depletion, p21 mRNA decay rate is strongly affected and its half-life is significantly increased, reaching 5.98±0.42 hrs (p-val<0.05). As a consequence, even after 8 hrs of ActD treatment, 43% of p21 mRNA is still present (Figure 7D). The slowing down of p21 mRNA decay rate following Fxr1-knockdown was further confirmed using 5,6-Dichlorobenzimidazole riboside (DRB), an adenosine analogue inhibiting mRNA synthesis (Figure S2). These data suggest that Fxr1-depletion increases endogenous p21 mRNA stability.
The γ fragment of p21 3′UTR contains a highly evolutionarily conserved G-quadruplex motif regulating its stability
The previous data support a negative role for FXR1P in the control of p21 mRNA stability via binding to the 561 nts long p21 3′UTR-γ portion. The next step was to determine the RNA motif responsible for FXR1P recognition. So far, two mRNA motifs have been described to be recognized by FXR1P: the ARE motif of TNFα mRNA  and the G-quadruplex present in FMR1 mRNA . Our in vitro data clearly indicate that the ARE present in the 3′UTR of p21 mRNA does not mediate the binding of FXR1P Isoe to p21 mRNA, we therefore looked for the presence of putative G-quadruplex motifs in the γ fragment of p21 3′UTR. For this purpose, we used the QGRS webtool  that indicated three putative G-quadruplexes spread along the sequence of the γ fragment (Figure S3), and notably a high-score central G-quadruplex motif (nts 931–955). It is worth noticing that this high-score putative G-quadruplex is located within a 51 nts G-rich region (position 918–955, 54% of G). To confirm the predicted G-quadruplex, we used the property of G-quadruplex forming regions to be detected by comparing reverse transcriptase elongation on RNA templates in the presence of either K+, Li+ or Na+ . Indeed, stabilization of G-quadruplex structures by K+, but not by Li+ or Na+, results in cation-dependent pauses detectable on a sequence gel. The experiments were performed on the full-length 3′UTR and on the γ fragment alone and allowed us to identify two strong (position 939 and 940) and two weak G-quadruplex pauses (position 955 and 969) in the 3′UTR of p21 mRNA (Figure 8A). Both the full-length and the γ fragment exhibited the same pauses, indicating that the γ fragment retains the ability to form the G-quadruplex structure in a comparable manner to the full-length native 3′UTR (Figure 8A). Alignment of sequences corresponding to G-rich regions of p21 distal 3′UTR in mouse and human indicate high evolutionary conservation of this portion of non-coding sequences (Figure 8B) and argues in favour of its functional importance.
(A) Cation-dependent termination of reverse transcription in the 3′-UTR full-length (FL) or γ fragment of p21 mRNA. Strong and weak pauses of reverse transcriptase (RT) are, respectively, indicated by large and thin arrows. Numbers correspond to positions of RT pauses, position +1 being the first nucleotide following the stop codon. (B) Localization and conservation of the G-quadruplex structure detected in (A) on the sequences of p21 3′UTR from Mus musculus (Mmu) and Homo sapiens (Hsa). (C) Scheme of the constructs used for luciferase assays bearing the conserved G-quadruplex of γ p21 mRNA (boxed) and two versions where the G-quadruplex has been deleted partially (Δ9) and fully (Δ38). (D) Effect of p21 3′UTR G-quadruplex and its deletions on Renilla luciferase (Ren) mRNA stability in C2C12 cells. Quantitative RT-PCR analysis of the levels of Ren mRNA normalised to Firefly (Luc) mRNA relative to the levels of the empty construct. The γ fragment bearing the G-quadruplex significantly increases Ren mRNA levels relative to empty vector. Partial or full deletion of the G-quadruplex sequence strongly increases Ren mRNA levels, both relative to empty vector and to the G-quadruplex bearing fragment. The results are presented as the mean of 4 experiments (±SEM). The asterisks * and # indicate p<0.05 respectively of the Wilcoxon test or of the Mann & Whitney test.
To explore the functional role of the G-quadruplex present in the 3′UTR of p21 mRNA, we constructed γ fragments mutants with partial (γΔ9) or full (γΔ38) deletion of the G-rich region containing the G-quadruplex (Figure 8C) that were cloned downstream of Renilla luciferase mRNA. Then, the levels of Renilla mRNA of the resulting constructs were assessed for each mutant in C2C12 cells. As previously shown in Figure 6B, the presence of the γ fragment did increase significantly the levels of Renilla mRNA, but partial or full deletion of the G-quadruplex potentiated the increase in the cognate mRNA levels (Figure 8D), mimicking the effect of Fxr1 knockdown in C2C12 cells (cf Figure 7B). These data argue in favour of a role of the G-quadruplex in mRNA stabilization that is potentiated by deletion of the binding site of FXR1P.
Over the last decade, studies in Fxr1-knockout models have inferred that FXR1P plays a critical role in myogenesis , , . However, even though FXR1P muscle-specific isoforms have unique RNA-binding properties , no specific mRNA targets and function have been identified so far for FXR1P in muscle. In this study, we have explored the functional consequences of the depletion of the FXR1P in myoblasts, with the purpose to understand its role in the early stage of myogenesis and in the cellular pathophysiology of FSHD, a human myopathy.
Cellular pathways affected by Fxr1-depletion
Microarray analysis of our myoblastic model inactivated for Fxr1 enabled to show that FXR1P depletion affects the expression of a wide range of mRNA species that control several cellular pathways. One of the most represented functional categories correspond to ‘skeletal and muscular system development’ and ‘skeletal and muscular disorders’, in line with the evoked role of FXR1P in myogenesis and its altered pattern of expression in two human myopathies: FSHD  and DM1 . Interestingly, the functional category ‘cell cycle’ appears also overrepresented in the affected functions, in particular, terms corresponding to ‘arrested in G0/G1 phase transition’ (related to the genes p21/Cdkn1a, HGF, IGF, IL6) actually reflect what we observed at the physiological level for Fxr1 inactivated myoblasts which remain blocked in the G0 phase, without undergoing further differentiation. Apart from p21, several mRNAs with altered levels in the absence of FXR1P seem to influence the functional categories affected and appear iteratively in our Ingenuity pathway analysis. These candidates for interaction with FXR1P in the context of myogenesis now deserve further investigation. Notably, Hepatocyte growth factor (Hgf) mRNA is significantly upregulated in the absence of FXR1P (Table S1, Figure 1E and 1F, Figure S1) and is known to play an essential role in the migration and proliferation of myogenic cells . Similarly, the Insulin-like growth factor 1 (Igf1) would be a relevant target of FXR1P in the muscle context, since Igf1 plays a key regulatory role in skeletal muscle development, as well as muscle fiber regeneration and hypertrophy . Finally, Cyclin-dependent kinase 15 (Cdk15) mRNA which, contrary to p21 mRNA, is downregulated in Fxr1-deficient myoblasts (Table S1, Figure 1E and 1F, Figure S1) would be an interesting candidate for regulation of cell-cycle progression by FXR1P. In this case, FXR1P would stabilize Cdk15 mRNA via recognition of a yet unknown specific motif. Murine and human Cdk15 mRNA are not annotated in the AREsite database  and therefore do not seem to bear a canonical AU-rich element sequence in their 3′UTR. However, analysis of the 3672 nts long human Cdk15 mRNA using QGRS G-quadruplex mapping webtool reveals the presence of 8 putative G-quadruplex sequences (Table S3), with 2 putative G-quadruplex in the 3′UTR that represent binding sites for FXR1P. To ascertain the importance of FXR1P in the regulation of its putative mRNA targets newly identified in this study, it would be worth investigating the presence of ARE sequences, G-quadruplexes RNA structures in their 3′ untranslated region.
Role of FXR1P/G-quadruplex mRNA complex in the destabilization of p21 mRNA
Adequate regulation of the balance between proliferation and cell cycle arrest of myoblasts is a crucial step during myogenesis. The decision to progress through a new division cycle appears primarily regulated before the G1 to S phase transition, with p21 upregulation playing an important role in this process by blocking the formation of proliferation-inducing Cyclin A/Cdk2-E2F complexes . In this context, p21 gene undergoes extensive regulation, both at the transcriptional and posttranscriptional level. Our data do not support a transcriptional mechanism for the maintenance of elevated p21 mRNA levels in Fxr1-depleted muscle cells. Indeed, in myoblasts, p21 is under the sole transcriptional control of the myogenic transcription factor MyoD that activates its promoter . Our microarray and quantitative RT-PCR analyses reveal that MyoD levels remain normal in Fxr1-deficient myoblasts (Figure 1E). Finally, in luciferase assays, Ren mRNA levels are increased when p21 mRNA G-quadruplex region is fused to its 3′UTR, even though this mRNA does not contain the endogenous promoter of p21/Cdkn1a gene (Figure 7B, Figure 8D). These evidences privilege an FXR1P-mediated posttranscriptional mechanism of regulation of p21 mRNA levels involving the binding of FXR1P.
In myoblasts, FXR1P long isoforms Isoe and Isof are most likely not playing a role in translational regulation, since they are detected in the nucleus and faintly in the cytoplasm but do not associate to polyribosomes , , , . On the other hand, we cannot exclude a mechanism involving translational inhibition via binding of small or medium isoforms of FXR1P to p21 mRNA to another motif, which may be located in the central part of p21 mRNA 3′UTR (β fragment) that activates translation in the absence of FXR1P (Figure 7A, 7B). This would be consistent with the previously described role of FXR1P small isoform Isoa in translational control . However, our data strongly support the fact that the FXR1P-dependant translational control of p21 mRNA is mainly regulated by FXR1P long isoforms, notably Isoe, via binding to a 3′UTR-located G-quadruplex motif (Figure 8).
To date, the G-quadruplex has been described to be a negative ,  or positive  regulator of translation, and a zip-code for dendritic transport and synaptic localization  depending on its location on the mRNA (e.g. 5′UTR or 3′UTR) (for review see ). We report here an evolutionary conserved G-quadruplex motif as a novel RNA-binding motif present in a G-rich region of the distal portion of p21 mRNA 3′UTR. This motif, distinct from the classical ARE present in the proximal portion of the 3′UTR , appears nevertheless to control the stability of p21 mRNA. Indeed, when fused to the 3′UTR of Renilla luciferase, the G-quadruplex induces an increase in Renilla mRNA levels, (Figure 7B, Figure 8D) and this effect is potentiated by deletion of the G-quadruplex (Figure 8D). Collectively, these data argue that the G-quadruplex of p21 mRNA 3′UTR participates in the control of mRNA stability via a mechanism involving FXR1P. A few reports describe the involvement of 3′UTR-located G-rich stretches as downstream sequence elements (DSE) promoting polyadenylation and leading to increased stability of mRNA when located downstream the polyadenylation site , . However, in the context of p21 mRNA, the G-quadruplex (position 918–955 nts) located upstream of p21 mRNA polyadenylation site (AAUAAA sequence in position 1309–1314 nts) could act as an upstream sequence elements (USE) promoting polyadenylation, as described for a U-rich sequence in Prothrombin mRNA 3′UTR . An alternate mechanism would involve that FXR1P long isoforms drive degradation of p21 mRNA via recruitment of microRNAs and the RISC complex. RNA interference is well described to occur in the cytoplasm, but it was recently shown that small non-coding RNAs can associate with complementary pre-mRNA target both in the nucleus and in the cytoplasm, by binding to Ago2 . The lattest is a key component of the RNA-Induced Silencing Complex (RISC)  and a well-known interactor of FXR1P in human cells , Xenopus oocytes , and in Drosophila , . Interestingly, p21 mRNA 3′UTR contains an evolutionarily conserved binding site for miR-22 100 nts upstream of the G-quadruplex motif (Figure S3). This microRNA was recently shown to regulate p21 mRNA levels  and is bound in vivo by Ago2 . In this context, Fxr1-depletion or p21 3′UTR G-quadruplex deletion could prevent recruitment of the RISC complex on p21 mRNA and contribute to increase its stability, ultimately leading to an accumulation of p21 mRNA and of the cognate protein.
In myoblasts, FXR1P is not the sole RNA-binding protein playing a key role in the regulation of p21 mRNA. Several reports demonstrate the importance of the proximal ARE of p21 mRNA 3′UTR- present in the α fragment- to control the stability of this mRNA. In myoblasts, the ARE-mediated stabilization of p21 mRNA is mediated by cooperative binding of HuR and hnRNPC1 , , while its decay is controled by KSRP . Members of the hnRNPE family of proteins, PCBP1 and 2, control the central part of p21 3′UTR -the β fragment- . Finally, another hnRNPE, PCBP4, binds and stabilizes the γ fragment , while we show in this study that binding of FXR1P to the G-quadruplex motif of p21 3′UTR-γ fragment destabilizes the mRNA. Here, we wish to propose a double system of regulation in which FXR1P and PCBP4 cooperate to regulate the levels of p21 using the distal 3′UTR while HuR, RNPC1 and KSRP use the ARE in the proximal part. These complex regulatory systems enable a fine-tuning of p21 mRNA levels, and our data indicate a prominent role for FXR1P as a modulator of p21 levels.
FXR1P control of p21 mRNA stability regulates myoblast cell-cycle exit
We report that, when FXR1P is depleted in the C2C12 cell line and in FSHD myoblasts, p21 levels increase (Figure 1, Figure 4). As a consequence, a subset of myoblasts becomes more permissive to cell cycle arrest, resulting in a reduced yield of myoblasts at each cycle of division (Figure 2, Figure 3). We also observed that the Cyclin-dependent kinase 15 (Cdk15) mRNA levels are decreased (Table S1; Figure 1E and 1F; Figure S1) it would be worth investigating whether its decreased levels also have an impact in this premature cell-cycle exit we observe in Fxr1-depleted myoblasts. Our data are in line with other studies in which overexpression of p21 in myoblasts is sufficient to trigger cell cycle exit, even in mitogenic medium , , . In our study, p21 upregulation upon Fxr1-depletion causes cell cycle exit without onset of differentiation. Indeed, the levels of the myogenic factors MyoD and Myogenin remain normal, as assessed by microarray (Table S1) and quantitative RT-PCR (Figure 1F). Moreover, we did not observe spontaneous myoblasts fusion into myotubes in Fxr1-knockdown cultures in normal growth conditions, which would be indicative of premature differentiation (Davidovic & Bardoni, unpublished data). Nevertheless, it would be worth investigating in details the impact of Fxr1-knockdown on the differentiation of C2C12 myoblasts. Indeed, our data predict that premature cell cycle exit of myoblasts in the absence of FXR1P decreases the pool of myoblasts available for differentiation. This would directly contribute to explain the reduced musculature detected in Fxr1-KO mice  and in xfxr1-knockdown Xenopus  at early stages of embryogenesis and development.
The fact that p21 mRNA is an mRNA target for FXR1P Isoe has also crucial implications for the understanding of the pathophysiology of myopathies. Indeed, splicing defects of the FXR1 gene in FSHD myoblasts leads to reduced expression of the long FXR1P Isoe, the one that specifically binds p21 3′UTR. We and others have shown that FSHD myoblasts exhibited higher levels of p21 than controls, under normal growth conditions (this study and , ). It is now tempting to speculate that depletion in FXR1P Isoe directly participates to the physiopathology of FSHD, by causing p21-mediated premature arrest of the cell cycle in FSHD myoblasts. Ultimately, this may limit the pool of myoblasts available for regeneration of muscle fibers, inducing progressive muscle wasting in FSHD patients. This hypothesis is supported by a study which demonstrates that p21 is essential for normal myogenic progenitor cell function in regenerating skeletal muscle . A similar scenario may be envisioned in the case of the mouse model of DM1 in which reduced expression of FXR1P Isoe was determined .
In conclusion, our study highlights for the first time the direct involvement of an RNA-binding protein, FXR1P, in a new pathway that regulates p21 levels to control myoblasts cell cycle exit. Perturbations of this pathway will have a strong impact in muscle development and implicates a new signal dependant on a 3′-UTR located G-quadruplex-RNA structure. In the future it will be important to explore the implication of FXR1P in pathophysiology of muscle disorders and the pleiotropic functions of FXR1P during myogenesis. Furthermore, our study opens new perspectives on the role of the other Fragile X related proteins in the control of cell cycle. Noteworthy, FMRP is known to recognize G-quadruplex mRNA structures and it would be tempting to speculate that FMRP could control p21-dependant cell cycle exit of neuronal progenitors during neurogenesis.
Materials and Methods
The C2C12 cell line, a subclone of the C2C4 murine myoblastic cell line , , was cultivated under confluence state in the conditions described by ATCC. C2C12 cells were transfected with siRNA targeting exon 14 or exon 6 of Fxr1 mRNA (see Table S1) and/or constructs using the Lipofectamine 2000 reagent (Invitrogen), according to the manufacturer's protocole. Control experiments were performed using commercially available control random siRNA of matching GC content (Invitrogen). Transfected cells were always analysed 48 hrs post transfection. mRNA decay experiments were performed by adding actinomycin D (Act D, 5 µg/mL) or 5,6-Dichlorobenzimidazole riboside (DRB, 50 µM) to culture medium for 0 to 8 hrs.
Human myoblasts derived from muscle biopsies of n = 3 FSHD patients and n = 3 controls of matching age and gender were described in . The procedures to generate myoblasts derived from human muscle biopsies were agreed by the French Health Authorities (AFSSAPS). Myoblasts cultures were established as previously described .
Gene expression profiling
Total RNA of C2C12 cells transfected with siFxr1 or siControl siRNAs was extracted using the RNeasy kit (Qiagen, Hilden, Germany). Integrity of RNA was assessed by using an Agilent BioAnalyser 2100 (Agilent Technologies) (RIN above 8). RNA samples were then labeled with Cy3 dye using the low RNA input QuickAmp kit (Agilent) as recommended by the supplier. 825 ng of labeled cRNA probe were hybridized on 8×60K high density SurePrint G3 gene expression mouse Agilent microarrays. Two biological replicates were performed for each experimental condition. The experimental data are deposited in the NCBI Gene Expression Omnibus (GEO) (http://www.ncbi.nlm.nih.gov/geo/) under the series record number GSE40577. Normalization of microarray data was performed using the Limma package available from Bioconductor (http://www.bioconductor.org). Inter slide normalization was performed using the quantile methods. Means of ratios from all comparisons were calculated and B test analysis was performed. Differentially expressed genes were selected based on a B-value above 0. Data from expression microarrays were analyzed for enrichment in biological themes (Gene Ontology molecular function and canonical pathways) and build biological networks using Ingenuity Pathway Analysis software (http://www.ingenuity.com/) and Mediante (http://www.microarray.fr:8080/merge/index), an information system providing information about probes and data sets.
Total RNA was extracted from myoblasts using the RNeasy kit (Qiagen, Hilden, Germany) and a reverse transcription (RT) reaction was performed using the Superscript II RT-PCR system (Invitrogen, Carlsbad, California, USA) according to the manufacturers' protocol. RT products were subjected to polymerase chain reaction (PCR). All primers were designed using the Primer 3 software (Table S4). Standard RT-PCR was performed using the Promega PCR Master Kit (Promega, Madison, Wisconsin, USA). Real-time PCR reactions were carried out using the Syber Green I qPCR core Kit (Eurogentec, Liège, Belgium) in a LightCycler system (Roche, USA). The comparative threshold cycle (Ct) for the amplicons of each sample was determined by the LightCycler software and normalised to the corresponding Ct of TATA Box Binding Protein (TBP) mRNA for endogenous p21 mRNA levels, and to the Ct of Firefly luciferase in the case of Renilla luciferase mRNA assessment. Finally, the 2-ΔΔCt method  was used to analyse the relative changes in the various studied mRNAs between C2C12 myoblasts transfected with control siRNA (Invitrogen) or anti-Fxr1 siRNA (Invitrogen), or between FSHD myoblasts and controls (n = 3). Data were expressed as means ±SEM. Each assay was performed in triplicate with n = 3–4 independent replicates.
Immunoblot and immunofluorescence
Cell extracts were analysed by western blotting as described previously , . Previously described primary antibodies against FXR1P were polyclonal rabbit antibody #830 (1∶5,000) and monoclonal 3FX (1∶500), the latter also cross-reacting with FXR2P . Anti-β-actin monoclonal antibody (Sigma) and anti-p21 polyclonal rabbit antibodies (Santa Cruz) were used respectively at 1∶10,000 and 1∶200. Digital acquisition of chemiluminescent signal was performed using the Las-3000 Imager system (Fujifilm). Quantitation of western-blot was performed using the ImageJ software and normalized to the β-actin signal.
Immunofluorescence was performed as described , using anti-FXR1P #830 polyclonal antibodies (1∶5,000; ) and anti-Ki67 monoclonal antibody (1∶100; Millipore). Secondary Alexa 594-coupled antibodies (Invitrogen, Carlsbad, California, USA) were used at 1∶250. After counterstaining with DAPI, coverslips were mounted on slides with anti-fading reagent and observed using a Zeiss Axioplan2 epifluorescence microscope equipped with a CoolSNAP HQ CCD cooled camera (Roper Scientific) or an Olympus FV10i confocal digital microscope. Micrographs were then analysed with ImageJ software.
Cell viability and FACS analysis
For viability assessment 48 hrs post transfection with anti-Fxr1 and control siRNAs, both attached cells and culture supernatant were collected and then incubated in the presence of propidium iodide (PI, 50 µg/mL). The incorporation of PI in dead cells was then analysed with a FACScan instrument (Becton, Dickinson). MTT proliferation assay was used to determine the proliferation ability of the cells as recommended by the manufacturer (Sigma). For cell cycle distribution assessment, cells were fixed in 70% ethanol, treated with RNAseA (50 µg/mL), stained with PI (50 µg/mL) and their DNA content was assessed using FACS analysis. For synchronisation experiments, cells were treated with 500 nM of the cell blocker mimosine for 8 hrs. Release from cell cycle blockade was performed for 16 hrs in growth medium before FACS analysis.
Human FXR1P Isoe recombinant protein His-tagged in the C–terminus was produced in bacteria using the pET21a/FXR1 Isoe construct , as described . The control RNA fragments used in this study: N19 (RNA sequence derived from FMR1 mRNA and containing a G-quadruplex forming structure) and N19Δ35 (N19 sequence in which the G-quadruplex is deleted) were cloned in pTL1 plasmid . The various fragments from p21 cDNA were amplified by RT-PCR of C2C12 cDNAs and cloned in the pGemTEasy system (Promega) using the primers described in Table S1, as advised by the manufacturer. For filter binding assay, N19 or p21 constructs were in vitro transcribed using T7 RNA polymerase (Promega), the RNA products being labeled by cotranscriptional incorporation of [γ−32 P]-ATP. Labeled RNAs were purified on a 1% low-melting agarose gel (Ambion). Labeled RNAs (50,000 c.p.m., 4 fmol) were renatured for 10 min at 40°C in binding buffer (50 mM Tris–HCl (pH 7.4), 1 mM MgCl2, 1 mM EDTA, 150 mM KCl, 1 mM DTT). In the presence of 2 U/mL of RNase inhibitors (RNasin, Invitrogen), 0,1 mg/mL of Escherichia coli total tRNA and 0.01% BSA, radiolabeled RNA were incubated to increasing amounts of FXR1P protein. RNA–protein complexes were allowed to form for 10 min on ice, filtered through MF-membranes (0.45 HA, Millipore) and washed with 2 mL binding buffer. Filters were air-dried and Cerenkov counting was used to assess the levels of remaining radioactivity on filters. Data were plotted as percentage of total RNA bound versus the protein concentration and one-site binding curve was drawn using the Prism 4 software.
UV-crosslinking and immunoprecipitation (CLIP)
To isolate mRNAs associated with FXR1P in vivo, UV-crosslinking and immunoprecipitations (CLIP) were performed with extracts of C2C12 cells using a protocol adapted from  and the #830 polyclonal antibody directed against the C-terminus of FXR1P . For each assay, 10 µg of polyclonal antiserum was used to immunoprecipitate 25×106 cells. An equivalent amount of unrelated rabbit IgGs (Sigma) were used as negative control. Approximately 1/20th of the homogenate and 1/4th of the immunoprecipitate were loaded on a 11% SDS–PAGE gel. Proteins transferred onto a 0.45 µm nitro-cellulose membrane were revealed using the 3FX antibody recognizing both FXR1P and FXR2P . mRNAs were extracted from C2C12 input lysate and immunoprecipitates using Trizol reagent (Invitrogen) according to the manufacturer's protocole and subjected to reverse transcription (RT) using the SuperscriptScript III RT-PCR system (Invitrogen). RT products were subjected to polymerase chain reaction (PCR), using a PCR Master Kit (Promega) and primers detailed in Table S4 specific for p21, Myogenin, MyoD and β-Tubulin mouse cDNAs. The PCR program consisted in 10 min. of initial denaturation at 95°C followed by 35 cycles −30 s. at 95°C, 30 s. at 58°C, 30 s. at 72°C- and a final elongation step of 10 min at 72°C. PCR products were visualised on a 2% TAE agarose gel and amplicon size was verified using the 1 Kb+ DNA ladder (Invitrogen).
Luciferase assays were performed using the pSiCheck2 system (Promega) according to the manufacturer's protocole. Briefly, the various fragments from p21-3′UTR cDNA (α, β and γ) were excised from the pGemTEasy vectors using the NotI site and inserted downstream of the Renilla luciferase cDNA using the NotI site of the pSiCheck2 vector. C2C12 cells were co-transfected in 96-well plates with the siRNA control or against Fxr1 and pSiCheck2 constructs. Luciferase assays were performed 48 hrs post transfection using the DualGlow Luciferase Kit (Promega) according to the manufacturer's protocole.
pTL1/FXR1Isoe plasmid was cloned as described in . The mutated version of this plasmid bearing 4 silent mutations in human FXR1 cDNA that impede recognition by siFxr1#1 was produced by site-directed mutagenesis using primers described in Table S4 and the QuickChange kit (Stratagene).
To compare numerical data, non-parametric Mann & Whitney test was used for small sample size (n<30) and a Student T-test was used when n>30. Wilcoxon non-parametric tests were used to assess significance of Renilla luciferase mRNA or activity levels variations between each fragment relative to the empty vector (arbitrarily set to 1). All statistical analysis and data graphs were performed with the Prism 4 software. Only significant differences are displayed on the graphs.
Confirmation of microarray mRNA candidates using a second siRNA targeting another constitutive exon of Fxr1 mRNA (exon 6). (A) Quantitative RT-PCR reveals a strong reduction of Fxr1 mRNA in C2C12 cells transfected with siRNA against Fxr1 (siFxr1#2) compared to siControl-transfected cells. (B) Western-blot analysis of untransfected (UT) and siFxr1-transfected cells (siFxr1#2) revealed with the antibody #3FX recognizing all isoforms of FXR1P reveals a strong depletion of all isoforms of FXR1P (short, medium and long) compared to control (siCtl), while the levels of FXR2P protein (asterisk, *) remain unchanged. β-tubulin (β-tub) signal is used to verify equal loading of lanes. (C) Quantitative-RT PCR analysis of a subset of mRNAs confirm that Sema7a, Mctp2, Asrg1, Cdkn1a/p21, Hgf, Dusp6 and Lbh mRNAs are significantly upregulated while Cdk15 mRNA is downregulated in Fxr1-depleted C2C12 myoblasts, confirming the microarray analysis and quantitative-RT PCR analysis using the first siFxr1 siRNA. Data are presented as means ± SEM of n = 4 experiments.
Confirmation that Fxr1-depletion increases the stability of endogenous p21 mRNA using a second transcription inhibitor. C2C12 transfected with siControl (empty squares) or siFxr1 (black squares) were treated with the transcription inhibitor 5,6-Dichlorobenzimidazole riboside (DRB) for 8 hrs. Percentages of remaining p21 mRNA at the various time points were determined by quantitative RT-PCR and normalised to levels before treatment (t0). During the first 5 hrs of treatment, p21 mRNA stability is clearly increased when FXR1P is knocked-down by siFxr1 transfection, as compared to siControl-transfected cells. At the dose used, DRB effect is reversible and transcription resumes after 5 hrs of treatment resulting in a progressive increase in p21 mRNA levels in both conditions.
Sequence analysis of p21 3′UTR γ fragment bound by FXR1P in search for G-quadruplexes and microRNA binding sites. The position and scores of the three putative G-quadruplexes structures predicted by the webtool QGRS  in the γ portion of murine p21 mRNA 3′UTR are boxed. The putative G-quadruplex located between nts 931–955 displays a high score of 38 and lies within a G-rich region (nts 918–968) in which G are highlighted by empty circles (°). A conserved binding site for miR-22/22-3p conserved among species is located in position 837–843 nts, as predicted by TargetScan webtool.
List of the 79 RefSeq annotated transcripts significantly modulated after Fxr1-depletion in myoblasts. NCBI RefSeq IDs give access to transcripts annotations. Logarithm (base 2) of the average intensity (AveExp) and logarithm (base 2) of the ratio siFxr1/siControl are represented. The subset of mRNAs further validated in quantitative RT-PCR are highlighted in bold.
Ingenuity Pathway Analysis of microarray data to highlight selectively affected pathways in Fxr1-depleted myoblasts. All the affected pathways ordered by p-value are presented in (A), while pathways specifically related to ‘skeletal muscle’ or ‘cell cycle’ are respectively presented in (B) and (C).
Prediction of G-quadruplexes present in human Cdk15 mRNA using QGRS webtool. G-quadruplexes displaying the highest scores are localized in the 5′UTR or coding sequence of the mRNA.
The authors gratefully acknowledge Ms. Julie Cazareth and Dr. Frederic Brau (IPMC, CNRS UMR7275, Valbonne, France) for excellent technical support concerning FACS analysis and quantification of microscopic images. The authors are also grateful to the GDR G-quadruplex Network.
Conceived and designed the experiments: LD PB BM SS HM BB. Performed the experiments: LD ND OK RT BM. Analyzed the data: LD OK BM HM PB BB. Contributed reagents/materials/analysis tools: SS. Wrote the paper: LD BB.
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