African Americans have the highest rate of mortality due to coronary heart disease (CHD). Although multiple loci have been identified influencing CHD risk in European-Americans using a genome-wide association (GWAS) approach, no GWAS of incident CHD has been reported for African Americans. We performed a GWAS for incident CHD events collected during 19 years of follow-up in 2,905 African Americans from the Atherosclerosis Risk in Communities (ARIC) study. We identified a genome-wide significant SNP (rs1859023, MAF = 31%) located at 7q21 near the PFTK1 gene (HR = 0.57, 95% CI 0.46 to 0.69, p = 1.86×10−08), which replicated in an independent sample of over 8,000 African American women from the Women's Health Initiative (WHI) (HR = 0.81, 95% CI 0.70 to 0.93, p = 0.005). PFTK1 encodes a serine/threonine-protein kinase, PFTAIRE-1, that acts as a cyclin-dependent kinase regulating cell cycle progression and cell proliferation. This is the first finding of incident CHD locus identified by GWAS in African Americans.
In the United States, African Americans are at high risk for coronary heart disease (CHD). Although environmental and social factors have a role, genetic factors also contribute to CHD risk and mortality. Research to identify genetic factors for CHD susceptibility has been carried out mostly in Europeans and European Americans and little has been done in African Americans. Genome wide association studies (GWAS) provide a means to identify susceptibility loci without any a priori assumptions about the functional importance of a gene. In this study, we used GWAS to identify a novel genomic region associated with incident CHD events in African Americans from the ARIC study and replicated this finding in a large sample of African American women. This region contains several genes, including PFTK1, that regulate cell cycle progression and cell proliferation. This is the first report of a susceptibility locus for incident CHD identified by GWAS in African Americans.
Citation: Barbalic M, Reiner AP, Wu C, Hixson JE, Franceschini N, Eaton CB, et al. (2011) Genome-Wide Association Analysis of Incident Coronary Heart Disease (CHD) in African Americans: A Short Report. PLoS Genet 7(8): e1002199. doi:10.1371/journal.pgen.1002199
Editor: Greg Gibson, Georgia Institute of Technology, United States of America
Received: March 1, 2011; Accepted: June 7, 2011; Published: August 4, 2011
Copyright: © 2011 Barbalic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The Atherosclerosis Risk in Communities Study is carried out as a collaborative study supported by National Heart, Lung, and Blood Institute contracts (HHSN268201100005C, HHSN268201100006C, HHSN268201100007C, HHSN268201100008C, HHSN268201100009C, HHSN268201100010C, HHSN268201100011C, and HHSN268201100012C), R01HL087641, R01HL59367, and R01HL086694; National Human Genome Research Institute contract U01HG004402; and National Institutes of Health contract HHSN268200625226C. Infrastructure was partly supported by Grant Number UL1RR025005, a component of the National Institutes of Health and NIH Roadmap for Medical Research. The WHI program is funded by the National Heart, Lung, and Blood Institute, National Institutes of Health, U.S. Department of Health and Human Services, through contracts N01WH22110, 24152, 32100-2, 32105-6, 32108-9, 32111-13, 32115, 32118-32119, 32122, 42107-26, 42129-32, and 44221. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Coronary heart disease (CHD) is the leading cause of death worldwide . In the United States, African Americans are the most vulnerable population with regard to CHD risk factors and mortality. A recent American Heart Association report showed that African Americans are twice as likely to die from a heart-related disease compared to other ethnicities . The presence of multiple CHD risk factors is 50% more likely in African Americans than in the population of European ancestry. Hypertension and diabetes are more prevalent and the highest rate of obesity is found in African American women . The factors underlying these disparities are not well understood. Socioeconomic status and health care accessibility play an important role . However, genetic factors are known to influence the risk of CHD  and population differences in the frequency and effects of these genetic factors also likely have a role.
Progress in the discovery of susceptibility genes for multiple chronic diseases and their risk factors has been made possible in recent years through genome-wide association studies (GWAS); African Americans were noticeably absent from most of these studies. GWAS have the advantage of discovering new genetic variants underlying a disease without a priori knowledge of gene location or function. More than 20 variants for CHD have been discovered in samples of European-descent so far –.
In this study, we took advantage of rich longitudinal data on CHD incident events in the Atherosclerosis Risk in Communities (ARIC) study and performed a GWAS of incident CHD events in African Americans. Results that reached pre-specified genome-wide significance were investigated in African Americans from the Women's Health Initiative (WHI) .
Genome-wide association analysis for incident CHD was carried out in African Americans from the ARIC study. After 19 years of follow-up, 362 individuals developed CHD and 2,543 individuals were free of CHD. Descriptive statistics for multiple cardiovascular disease risk factors at the baseline examination are provided in Table 1. Three loci reached the pre-specified genome-wide significance threshold (p<5×10−8) (Table 2) and were considered for replication in a sample of African American women from WHI. Results are shown in Table 2. For all variants, the direction of effect was consistent in both studies, but only the variant rs1859023 reached statistical significance in WHI (Table 2). The rs1859023 minor allele A (frequency = 0.31) had a protective effect on CHD risk with a hazard ratio (HR) of 0.57 (95% CI 0.46 to 0.69, p = 1.86×10−08) in ARIC and 0.81 (95% CI 0.70 to 0.93, p = 0.005) in WHI. After adjustment for CHD risk factors (smoking, diabetes, LDL, BMI and hypertension), the result was only slightly less significant (HR = 0.59, 95% CI 0.48 to 0.73, p = 8.53×10−07 in the ARIC study). After exclusions of coronary revascularization procedures from the ARIC case definition, the association became stronger (HR 0.53, 95% CI 0.43 to 0.65, p = 2.95×10−09), possibly due to a more homogenous definition of CHD. The pooled hazard ratio of incident CHD for combined ARIC and WHI data was 0.72 (95% CI 0.64 to 0.81, p = 1.29×10−08).
rs1859023 was not significantly associated with any of the traditional CHD risk factors at the baseline ARIC examination (LDL, HDL, SBP, DBP and BMI, data not shown). However, average thickness of the carotid artery, a common measure of subclinical atherosclerosis, was significantly different among rs1859023 genotypes (mean difference in carotid artery thickness per copy of the minor allele β = −0.0168, SE = 0.0047, p = 3.39×10−04) which suggest a role for this variant in the atherosclerotic process. When including carotid artery wall thickness as a covariate in the analysis of incident CHD attenuated but did not abolish the association with rs1859023 (HR = 0.59, 95% CI 0.48 to 0.73, p = 1.58×10−06). We attepted to replicate this observation by testing the assocation of rs1859023 and right coronary fatty streak area (% of intimal surface area) in the PDAY study consisting of 1,452 African Americans and 1,342 European Americans but the results did not reach statistical significance using a one-sided test. In the ARIC European Americans, rs1859023 was not significantly associated with incident CHD. The smallest p-value in the region surrounding rs1859023 (±50 kb) was at rs17869240 (p = 0.01, MAF = 0.07).
rs1859023 is located in an intergenic region in proximity to the PFTK1, CLDN12 and GTPBP10 genes (Figure 1). PFTK1, also known as CDK14, encodes a serine/threonine-protein kinase PFTAIRE-1 that acts as a cyclin-dependent kinase regulating cell cycle progression and cell proliferation . It is highly expressed in heart tissue . In the ARIC study, rs1859023 is in loose linkage disequilibrium (LD) with other SNPs in the region (r2< = 0.4). In the Yoruba HapMap data, there are only 3 SNPs with LD greater than r2 = 0.8, all located within a very short distance of rs1859023 (Figure 1) which implies that the tagging region of rs1859023 is very narrow in African-derived populations. Given the location of the rs1859023 5′ to the PFTK1 gene, these data imply that rs1859023 may affect gene expression. To test this hypothesis, we undertook expression QTL analyses using the resources provided at the SCAN – SNP and CNV Annotation Database (http://www.scandb.org/newinterface/about.html) . rs1859023 predicts the expression of 6 genes with p-values less than 10−4 (Table 3). Interestingly, the evidence of rs1859023 predicting the gene expression is found only in the Yoruba population.
A variant close to PFTK1, rs10499903, located ∼60 kb from rs1859023 was associated with ankle brachial index (ABI) in European Americans from the Framingham Heart Study (FHS) . Given the different ethnic backgrounds of the two studies, and expected difference of LD patterns within the region between two samples, the Framingham result strengthens our finding and further suggests a role for the PFTK1 gene region in the atherosclerotic process.
Some of European-discovered CHD genes have been reported to also influence CHD in African Americans . However, this is the first reported GWAS finding of a CHD risk locus in African Americans. Although the sample size is less than contemporary GWAS publications in European-Americans (i.e. often exceeding 100,000 individuals (e.g. ), we have combined all of the available well-powered incident CHD data in African Americans with genotype data and are able to present results based on 133,415 person-years of follow-up. In addition, supporting evidence is provided by the association with subclinical atherosclerosis and expression QTL analyses. In conclusion, we have identified a region near the PFTK1 gene as being associated with incident CHD and subclinical atherosclerosis in African Americans. Further studies are needed to examine the cellular or metabolic mechanisms underlying this association, and large population-based studies of minority populations are necessary to more fully understand the impact of genetic factors on multiple phenotypes in those that bear a disproportionate burden of disease.
Materials and Methods
The ARIC (Atherosclerosis Risk in Communities) study is a population-based prospective cohort study of cardiovascular disease and its risk factors . ARIC includes 15,792 persons aged 45–64 years at baseline (1987–89), randomly chosen from four US communities. Of these individuals, 4,266 are self-reported African Americans. Cohort members completed four clinic examinations, conducted approximately three years apart between 1987 and 1998, and followed with annual phone interviews since 1987.
Incident CHD in ARIC was ascertained by contacting participants annually, identifying hospitalizations and deaths during the prior year, and by surveying discharge lists from local hospitals and death certificates from state vital statistics offices for potential cardiovascular events . A CHD event was defined as a validated definite or probable hospitalized MI, a definite CHD death, an unrecognized MI defined by ARIC ECG readings, or coronary revascularization. Participants were excluded from analyses if they had a positive or unknown history of prevalent stroke, transient ischemic attack/stroke symptoms, or CHD at the initial visit and/or being of non–African American ethnicity. Real-time, B-mode ultrasound was used to evaluate the carotid arterial intima-media wall thickness as an indicator of atherosclerosis in the ARIC study and the detailed description of its measurement is described elsewhere .
Genotyping was done in 15,020 ARIC participants using the Affymetrix Genome-Wide Human SNP Array 6.0. A total of 3,182 individuals remained after excluding individuals of non African American ethnicity, subjects who did not consent DNA use, unintentional duplicates with higher missing genotype rates, suspected mixed/contaminated samples, scans from one problem plate, samples with a mismatch between called and phenotypic sex, samples with genotype mismatch with 39 previously genotyped SNPs, suspected first-degree relative of an included individual, and genetic outliers based on average IBS statistics and principal components analysis using EIGENSTRAT. SNPs were excluded due to having no chromosome location, being monomorphic, having a call rate <95% and HWE-p<10−5. In this analysis, we considered only variants with a MAF greater than 10%.
Cox proportional hazards models with adjustment for age, gender and the first three principal components derived from EIGENSTRAT were used to estimate CHD hazard rate ratios (HRs) over a 19-year period (362 cases) under an additive genetic model. These analyses were done using PLINK and an R application for survival regression analyses. We define as “genome-wide significant” all associations with p<5×10−8. We define replication to be a significant (p<0.05) and directionally consistent association in an independent sample.
The WHI has two major components: (1) a clinical trial that enrolled and randomized 68,132 women ages 50–79 into at least one of three clinical trials; and (2) an observational study that enrolled 93,676 women ages 50–79 into a parallel prospective cohort study . WHI participants were recruited from 1993–1998 at 40 clinical centers across the U.S. During follow-up, incident CHD events were adjudicated locally and centrally from medical records including hospital discharge summaries, ICD-9 codes, diagnostic, laboratory, surgical, and pathology reports by trained physicians blinded to randomized intervention and exposure status . In the WHI replication sample, CHD was defined as MI, coronary revascularization, hospitalized angina, or CHD death. Definite and probable nonfatal MI required overnight hospitalization and was defined according to an algorithm based on standardized criteria using cardiac pain, cardiac enzymes and troponin levels, and ECG findings. CHD death was defined as death consistent with underlying cause of CHD plus one or more of the following: hospitalization for MI within 28 days prior to death, previous angina or myocardial infarction, death due to a procedure related to CHD, or a death certificate consistent with underlying cause of atherosclerotic CHD.
Of a total of 26,045 (17%) women from minority groups, 8,515 self identified African American women who had consented to genetic research were eligible for the WHI GWAS project. Genotyping was performed on the Affymetrix 6.0 array. After excluding samples due to genotyping failure, cryptic relatedness, and discrepancy between genetic ancestry and self-reported race, there were 8,421 WHI African Americans. Participants were further excluded from analyses if they had a positive or unknown history of prevalent stroke, transient ischemic attack/stroke symptoms, or CHD at the initial visit. A total of 862 incident first CHD events occurred among 8,155 eligible African American women without baseline CHD (Table 4). The mean age at study entry was 61.6+/−7.0 years (range 50–79). The mean baseline age of the cases was 64.2+/−7.2, and the mean baseline age of the non-cases was 61.3+/−6.9. The mean time to CHD event was 5.29+/−3.19 years. The mean age at CHD event was 69.5+/−7.5 years.
This study was approved by the participating institutional IRBs, and all ARIC and WHI participants provided written informed consent, involving the sharing of data with the scientific community.
The Pathobiolobical Determinants of Atherosclerosis in Youth (PDAY) study is composed of subjects who were 15 to 34 years of age when they died of non-CVD related causes (accidents, homicides or suicides). The purpose of PDAY was to evaluate early development of atherosclerosis . For this replication analysis, we genotyped rs1859023 in 2,794 individuals from PDAY - 1,452 African Americans and 1,342 European Americans and tested the association with fatty streak area in the right coronary artery.
The authors thank the staff and participants of the ARIC study for their important contributions. The authors thank the WHI investigators and staff for their dedication and the study participants for making the program possible. A listing of WHI investigators can be found at http://www.whiscience.org/collaborators/investigators.php.
Conceived and designed the experiments: MB APR EB. Analyzed the data: MB APR CW. Contributed reagents/materials/analysis tools: APR JEH CBE GH DC TM EB. Wrote the paper: MB APR EB NF.
- 1. WHO (2010) Global burden of coronary heart disease. http://wwwwhoint/cardiovascular_diseases/en/cvd_atlas_13_coronaryHDpdf.
- 2. Lloyd-Jones D, Adams RJ, Brown TM, Carnethon M, Dai S, et al. Heart disease and stroke statistics–2010 update: a report from the American Heart Association. Circulation 121: e46–e215.
- 3. Clark LT (2005) Issues in minority health: atherosclerosis and coronary heart disease in African Americans. Med Clin North Am 89: 977–1001, 1994.
- 4. Lusis AJ, Mar R, Pajukanta P (2004) Genetics of atherosclerosis. Annu Rev Genomics Hum Genet 5: 189–218.
- 5. Kathiresan S, Voight BF, Purcell S, Musunuru K, Ardissino D, et al. (2009) Genome-wide association of early-onset myocardial infarction with single nucleotide polymorphisms and copy number variants. Nat Genet 41: 334–341.
- 6. Tregouet DA, Konig IR, Erdmann J, Munteanu A, Braund PS, et al. (2009) Genome-wide haplotype association study identifies the SLC22A3-LPAL2-LPA gene cluster as a risk locus for coronary artery disease. Nat Genet 41: 283–285.
- 7. Erdmann J, Grosshennig A, Braund PS, Konig IR, Hengstenberg C, et al. (2009) New susceptibility locus for coronary artery disease on chromosome 3q22.3. Nat Genet 41: 280–282.
- 8. Ozaki K, Sato H, Inoue K, Tsunoda T, Sakata Y, et al. (2009) SNPs in BRAP associated with risk of myocardial infarction in Asian populations. Nat Genet 41: 329–333.
- 9. Samani NJ, Erdmann J, Hall AS, Hengstenberg C, Mangino M, et al. (2007) Genomewide association analysis of coronary artery disease. N Engl J Med 357: 443–453.
- 10. Samani NJ, Deloukas P, Erdmann J, Hengstenberg C, Kuulasmaa K, et al. (2009) Large scale association analysis of novel genetic loci for coronary artery disease. Arterioscler Thromb Vasc Biol 29: 774–780.
- 11. Schunkert H, Konig IR, Kathiresan S, Reilly MP, Assimes TL, et al. Large-scale association analysis identifies 13 new susceptibility loci for coronary artery disease. Nat Genet 43: 333–338.
- 12. (1998) Design of the Women's Health Initiative clinical trial and observational study. The Women's Health Initiative Study Group. Control Clin Trials 19: 61–109.
- 13. Shu F, Lv S, Qin Y, Ma X, Wang X, et al. (2007) Functional characterization of human PFTK1 as a cyclin-dependent kinase. Proc Natl Acad Sci U S A 104: 9248–9253.
- 14. Yang T, Chen JY (2001) Identification and cellular localization of human PFTAIRE1. Gene 267: 165–172.
- 15. Gamazon ER, Zhang W, Konkashbaev A, Duan S, Kistner EO, et al. SCAN: SNP and copy number annotation. Bioinformatics 26: 259–262.
- 16. O'Donnell CJ, Cupples LA, D'Agostino RB, Fox CS, Hoffmann U, et al. (2007) Genome-wide association study for subclinical atherosclerosis in major arterial territories in the NHLBI's Framingham Heart Study. BMC Med Genet 8: Suppl 1S4.
- 17. Kral BG, Mathias RA, Suktitipat B, Ruczinski I, Vaidya D, et al. A common variant in the CDKN2B gene on chromosome 9p21 protects against coronary artery disease in Americans of African ancestry. J Hum Genet 56: 224–229.
- 18. Teslovich TM, Musunuru K, Smith AV, Edmondson AC, Stylianou IM, et al. Biological, clinical and population relevance of 95 loci for blood lipids. Nature 466: 707–713.
- 19. ARIC (1989) The Atherosclerosis Risk in Communities (ARIC) Study: design and objectives. The ARIC investigators. Am J Epidemiol 129: 687–702.
- 20. Curb JD, McTiernan A, Heckbert SR, Kooperberg C, Stanford J, et al. (2003) Outcomes ascertainment and adjudication methods in the Women's Health Initiative. Ann Epidemiol 13: S122–128.
- 21. Hixson JE (1991) Apolipoprotein E polymorphisms affect atherosclerosis in young males. Pathobiological Determinants of Atherosclerosis in Youth (PDAY) Research Group. Arterioscler Thromb 11: 1237–1244.
- 22. Pruim RJ, Welch RP, Sanna S, Teslovich TM, Chines PS, et al. LocusZoom: regional visualization of genome-wide association scan results. Bioinformatics 26: 2336–2337.
- 23. Johnson AD, Handsaker RE, Pulit SL, Nizzari MM, O'Donnell CJ, et al. (2008) SNAP: a web-based tool for identification and annotation of proxy SNPs using HapMap. Bioinformatics 24: 2938–2939.