Metarhizium spp. are being used as environmentally friendly alternatives to chemical insecticides, as model systems for studying insect-fungus interactions, and as a resource of genes for biotechnology. We present a comparative analysis of the genome sequences of the broad-spectrum insect pathogen Metarhizium anisopliae and the acridid-specific M. acridum. Whole-genome analyses indicate that the genome structures of these two species are highly syntenic and suggest that the genus Metarhizium evolved from plant endophytes or pathogens. Both M. anisopliae and M. acridum have a strikingly larger proportion of genes encoding secreted proteins than other fungi, while ∼30% of these have no functionally characterized homologs, suggesting hitherto unsuspected interactions between fungal pathogens and insects. The analysis of transposase genes provided evidence of repeat-induced point mutations occurring in M. acridum but not in M. anisopliae. With the help of pathogen-host interaction gene database, ∼16% of Metarhizium genes were identified that are similar to experimentally verified genes involved in pathogenicity in other fungi, particularly plant pathogens. However, relative to M. acridum, M. anisopliae has evolved with many expanded gene families of proteases, chitinases, cytochrome P450s, polyketide synthases, and nonribosomal peptide synthetases for cuticle-degradation, detoxification, and toxin biosynthesis that may facilitate its ability to adapt to heterogenous environments. Transcriptional analysis of both fungi during early infection processes provided further insights into the genes and pathways involved in infectivity and specificity. Of particular note, M. acridum transcribed distinct G-protein coupled receptors on cuticles from locusts (the natural hosts) and cockroaches, whereas M. anisopliae transcribed the same receptor on both hosts. This study will facilitate the identification of virulence genes and the development of improved biocontrol strains with customized properties.
Aside from playing a crucial role in natural ecosystems, entomopathogenic fungi are being developed as environmentally friendly alternatives for the control of insect pests. We conducted the first genomic study of two of the best characterized entomopathogens, Metarhizium anisopliae and M. acridum. M. anisopliae is a ubiquitous pathogen of >200 insect species and a plant growth promoting colonizer of rhizospheres. M. acridum is a specific pathogen of locusts. Important findings of this study included: 1) Both M. anisopliae and M. acridum have a very large number of genes encoding secreted proteins, and many of these play roles in fungus-insect interactions. 2) M. anisopliae has more genes than M. acridum, which may be associated with adaptation to multiple insect hosts. 3) Unlike M. acridum, the M. anisopliae genome contains many more transposase genes and shows no evidence of repeat-induced point mutations. The lack of repeat-induced mutations may have allowed the lineage-specific gene duplications that have contributed to its adaptability. 4) High-throughput transcriptomics identified the strategies by which these fungi overcome their insect hosts and achieve specificity. These genome sequences will provide the basis for a comprehensive understanding of fungal–plant–insect interactions and will contribute to our understanding of fungal evolution and ecology.
Citation: Gao Q, Jin K, Ying S-H, Zhang Y, Xiao G, Shang Y, et al. (2011) Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum. PLoS Genet 7(1): e1001264. https://doi.org/10.1371/journal.pgen.1001264
Editor: Mark Achtman, Environmental Research Institute, University College Cork, Ireland
Received: July 26, 2010; Accepted: December 1, 2010; Published: January 6, 2011
Copyright: © 2011 Gao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: C Wang, Y Pei, and M-G Feng were supported by the Ministry of Science and Technology of China (grant no. 2009CB118904). C Wang was also supported by the Knowledge Innovation Program from Chinese Academy of Sciences (grant no. KSCX2-YW-G-037) and the National Hi-Tech Research and Development Program of China (grant no. 2009AA10Z112). Y Xia was supported by the Natural Science Foundation of China (grant no. 30771446). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Most fungi with sequenced genomes are plants pathogens or saprophytes. However, there are also thousands of entomopathogenic fungal species that play a crucial role in controlling insect populations. The genus Metarhizium includes the best studied entomopathogenic fungi at the molecular and biochemical level. They have a world-wide distribution from the arctic to the tropics and colonize an impressive array of environments including forests, savannahs, swamps, coastal zones and deserts . Metarhizium species are amongst the most abundant fungi isolated from soils with titers reaching 106 conidia per gram in grasslands . The genus contains M. anisopliae, which has a broad host range, as well as specialists, such as the locust-specific pathogen M. acridum. These two species in particular have emerged as excellent model organisms to explore a broad array of questions in ecology and evolution, host preference and host switching, and to investigate the mechanisms of speciation. In addition, both M. anisopliae and M. acridum have been at the forefront of efforts to develop biocontrol alternatives to chemical insecticides in agricultural and disease-vector control programs, and many commercial products are on the market or under development –.
Our knowledge of the ecological impact of M. anisopliae and its potential as a biocontrol agent has recently been enhanced by the discovery that it colonizes plant roots where it may simultaneously act as a biofertilizer and biopesticide to boost plant growth . Consistent with its broad lifestyle options, M. anisopliae exhibits an extremely versatile metabolism, enabling growth under various environmental conditions, with sparse nutrients and in the presence of compounds lethal to other fungi . As the asexual stages (anamorphs) of medicinally valued Cordyceps spp. , Metarhizium spp. are prolific producers of enzymes and diverse secondary metabolites with activities against insects, fungi, bacteria, viruses and cancer cells , , . In addition, the enzymes from Metarhizium spp. are frequently exploited as industrial catalysts , . M. anisopliae has also been used in studies on the immune systems of invertebrate model hosts to provide insights into emerging human pathogens , and it is a developing model for studies on aging , .
In contrast to the versatile M. anisopliae, the specialist M. acridum is specific for certain locusts and grasshoppers . However, like M. anisopliae, it is a producer of diverse cell types (e.g., conidia, hyphae, appressoria, unicellular blastospores, and multi-cellular hyphal bodies) that facilitate the infection of target insects via adhesion and penetration of the host cuticle, proliferation within tissues and the haemolymph, and eventual eruption through the host cadaver (Figure 1). M. acridum is mass produced and used on a large scale for locust control , whereas few other biological control agents have been such a commercial success because of poor efficacy compared to chemicals .
(A) A germinating conidium producing an appressorium. (B) Mycelia attacked by hemocytes after cuticular penetration. (C) Budding yeast-type cells (blastospores) produced by the fungus to facilitate dispersal in insect hemocoel. (D) Cadaver showing emerging hyphae producing conidia (E). CO, conidium; AP, appressorium. Bar, 5 µm.
Although recent advances have identified the functions of several pathogenicity genes – and technical developments improved the virulence of M. anisopliae , , the need to understand these fungi and expand their biotechnological potential requires sequenced genomes of M. anisopliae and M. acridum. Sequencing two related species that have evolved very different lifestyles will increase their utility as models, and provide insights into the evolution of pathogenicity. Such sequences will also allow for more rapid identification of genes encoding biologically active molecules and genes responsible for interactions between fungi, plants and insects. These findings could be further translated into the development of improved strains with customized properties that could potentially function as comprehensive plant symbionts to improve plant establishment and sustainable agriculture, particularly on marginal lands.
Genome sequencing and general features
The genomes were each shotgun sequenced to ∼100× coverage. The M. anisopliae genome (strain ARSEF 23) was assembled into 176 scaffolds (>1 kb; N50, 2.0 Mb) containing 1,271 contigs with a total size of 39.0 Mb (loci tagged as MAA). The M. acridum genome (strain CQMa 102) was assembled into 241 scaffolds (>1 kb; N50, 329.5 kb) containing 1,609 contigs with a genome size of 38.0 Mb (loci tagged as MAC) (Table 1). These assemblies closely correspond to the genome sizes of other Ascomycetes (Table S1). By mapping >6,000 unique expressed sequenced tagged sequences to the scaffolds, each genome was estimated to be >98% complete. M. anisopliae and M. acridum were predicted to have 10,582 and 9,849 protein coding genes, respectively, which is similar to the coding capacity of other Ascomycetes (Table S1). We examined homology relationships between M. anisopliae and M. acridum, and a set of eight other ascomycete genomes (Figure 2A). The results indicated that ∼90% of the genes in both Metarhizium genomes have homologs (E≤1×10−5) in other Ascomycetes. In addition, M. anisopliae has 398 (3.8%) genes with matches restricted to M. acridum (Metarhizium-restricted genes) and 263 (2.5%) orphan sequences. M. acridum has 219 (2.2%) orphan sequences (Figure 2A). Further analysis of the M. anisopliae orphans showed that 21.3% had matches in bacteria, 3.4% in animals and 3.8% in viruses. Similarly, 13.3%, 5.5% and 2.7% of the M. acridum orphans had matches in bacteria, animals and viruses, respectively, consistent with possible horizontal gene transfer events.
(A) Predicted proteins in M. anisopliae (MAA) and M. acridum (MAC) were compared with the genome encoding proteins of Aspergillus nidulans (AN), A. fumigatus (AF), Neurospora crassa (NC), Magnaporthe oryzae (MO), Fusarium graminearum (FG), Epichloë festucae (EF), Botrytis cinerea (BC) and Sclerotinia sclerotiorum (SS). The diagram was constructed with a cut off E-value <1×10−5. (B) Dot blot of M. anisopliae and M. acridum using ordered scaffold data. (C) Phylogenetic tree constructed using the Dayhoff amino acid substitution model showing the evolutionary relationships of 16 fungal species. MY = million years.
The proportion of genes encoding secreted proteins is remarkably large, being 17.6% (1,865 proteins) in M. anisopliae and 15.1% (1,490 proteins) in M. acridum as compared to 7–10% in plant pathogens  and ∼5% in N. crassa  or A. nidulans . As expected, many of the secreted proteins are in families which could have roles in colonization of insect tissues, such as proteases (Table S2). However, 32.2% of M. anisopliae and 28.7% of M. acridum secreted proteins had no conserved domains or functionally characterized homologs. Of these, ∼22% were Metarhizium-restricted genes and ∼4% were orphan genes in either genome.
Syntenic and phylogenetic relationships
Pairwise comparison indicated that the two Metarhizium genome structures have large areas of synteny (Figure 2B, Figure S1A). The lineage specific regions of M. anisopliae and M. acridum contain high densities of transposases, species-specific genes, genes encoding proteins with unknown functions and pseudogenes (Figure S1B). Similar lineage-specific regions were found in Fusarium spp. . Ninety nine percent of the M. anisopliae genome comprises non-repetitive sequences, and the orthologs shared with the M. acridum genome display an average 89.8% amino acid identity. The two Metarhizium species are therefore more closely related than the three Aspergillus species A. nidulans, A. fumigatus and A. oryzae which share only 68% average sequence identity . A phylogenomic analysis revealed that M. anisopliae and M. acridum lineages diverged about 33–43 million years (MY) ago and are most closely related to the mutualistic plant endophyte Epichloe festucae (divergence time 88–114 MY) and to the wheat head blight fungus Fusarium graminearum (divergence time 144–187 MY) (Figure 2C).
Transposases and repeat-induced point mutation
The specialist M. acridum harbors more repetitive elements than M. anisopliae but the latter has many more transposases (Table S2). Most of these are DNA transposases (97/148 in MAA; 12/20 in MAC), with subclasses hAT (45/97) and Helitron (26/97) being particularly abundant in M. anisopliae. The Copia (17) and LINE (26) retrotransposons are also abundant in the genome of M. anisopliae, while M. acridum has only three LINE elements and does not contain Copia (Figure 3A). Transcriptome analysis (see below) showed that most (>65%) of the transposase genes were transcribed by the Metarhizium hyphae during the infection process (Table S3).
Families of transposase genes (A) and estimation of RIP (B) in M. anisopliae and M. acridum.
The number of putative transposases in the M. acridum genome is lower by at least a factor of five than in most Ascomycetes, including M. anisopliae (Table S2). This could be explained by repeat induced point mutations (RIP) introducing CpG to TpA transitions in duplicated sequences during the sexual cycle . This mutational bias is observed in M. acridum (RIP index, 2.17) but not in M. anisopliae (RIP index, 1.09) (Figure 3B). Consistent with Neurospora crassa which has efficient RIP , the genome of M. acridum contained twice as many duplicated pseudogenes (254 versus129) as did that of M. anisopliae. The M. anisopliae genome contains more processed and fragmented pseudogenes caused by mobile elements (234 versus 186), consistent with transposons making a greater contribution to genetic instability in M. anisopliae (Table S4). The production of stable biocontrol agents for commercialization might therefore benefit from disabling transposable elements.
Virulence associated genes
An InterproScan analysis identified 2,710 protein families (containing 7,178 proteins) in M. anisopliae and 2,658 families (containing 6,615 proteins) in M. acridum. A stochastic birth and death model  showed that relative to M. acridum, 42 families including transporters, transcription factors, cytochrome P450s, proteases and lipases were expanded and three families (protein kinase, aminotransferase and transpeptidase) were contracted in M. anisopliae (Table S5). This resulted in M. anisopliae having more genes in most functional categories except for those involved in signal transduction (Figure 4, Table 2).
Each circle represents the relative fraction of genes represented in each of the categories for each genome. The gene numbers are also shown.
To find potential virulence-associated genes, a whole genome blast analysis was conducted against the pathogen-host interaction (PHI) gene database, a collection of experimentally verified pathogenicity, virulence and effector genes from fungi, oomycetes and bacteria . We identified 1,828 putative PHI genes in M. anisopliae (17.3% of its genes, belonging to 383 protein families) and 1,629 putative PHI genes in M. acridum (16.5%, 371 families), of which 1,331 genes were orthologous. Although there are no entries from entomopathogenic fungi in the PHI-base, we proceeded on the assumption that the proof of pathogenicity/virulence of a gene in one fungus also suggests a pathogenicity/virulence function in other fungi . In accordance with this assumption, the search of the PHI database yielded several already characterized M. anisopliae pathogenicity determinants, including subtilisins (see below) and hydrophobins (small cell wall proteins) that have pleiotropic functions in M. anisopliae including attachment of spores to hydrophobic surfaces . The class 2 (MAA_01182 and MAC_09507) and class 1 (MAA_10298 and MAC_04376) hydrophobins had significant similarity with PHI sequences from plant pathogenic fungi. The previously characterized adhesin, MAD1 (MAA_03775) required for specific binding to insect host surfaces , resembled EAP1 (PHI acc: 517) from the human pathogen Candida albicans. However, the adhesin MAD2 (MAA_03807) required for binding to plant surfaces , had no significantly similar sequence in the PHI database. Orthologs to both MAD1 (MAC_00987) and MAD2 (MAC_00953) were found in the M. acridum genome.
Using the PHI-base content with a focus on ascomycetes, Sexton and Howlett found many parallels in the infection mechanisms used by plant and animal pathogens . To determine how many plant pathogen PHI genes are also found in Metarhizium, we screened the F. graminearum and M. oryzae genomes against the PHI-base and identified 2,053 genes (in 398 families) and 1,713 genes (in 427 families), respectively, representing about 16% of gene contents in these two fungi (Table S6). Approximately, 70% of these genes are orthologous to PHI sequences in M. anisopliae and M. acridum. Fewer Metarhizium orthologs were found in animal pathogenic fungi such as C. albicans, which could be explained by Metarhizium being more closely related to plant pathogens (Figure 2C) as well as the animal pathogens lacking appressoria (infection structures) during host penetration .
Gene families involved in degrading insect cuticles
Insect pathogens such as Metarhizium spp. need to penetrate the protein-chitin rich insect cuticle and solubilize host tissues for nutrition. Therefore, they would be expected to secrete large numbers of degradative enzymes. Indeed, M. anisopliae has more genes encoding secreted proteases (132) than other sequenced fungi (Table S2). The trypsin family has the highest relative expansion among the proteases with 32 genes in M. anisopliae, almost twice as many as M. acridum and 6 to 10 times as many as the other taxa evaluated (Figure 5A, Table S2). A chymotrypsin (MAA_07484) that might have been imported from bacteria through horizontal gene transfer  and two trypsins that were recently duplicated in M. anisopliae (MAA_05135 and MAA_05136) are missing from the M. acridum genome (Table S7). Subtilisins (55 in MAA and 43 in MAC; 7 to 31 in other fungi) (Figure 5B, Table S8) and aspartyl proteases (33 in MAA and 25 in MAC; 9 to 21 in other fungi) (Table S9) are also expanded in M. anisopliae due to lineage-specific duplications (Figure S1C). Most of the Metarhizium subtilisins (48 in MAA and 37 in MAC) and aspartyl proteases (27 in MAA and 23 in MAC) had significant matches in the PHI-base. Subtilisins assist in the infection processes of M. anisopliae by degrading host cuticles, providing nutrition and disabling antimicrobial peptides . The importance of Metarhizium aspartyl proteinases has not been demonstrated but they resemble the aspartyl proteases that assist the human pathogen C. albicans by degrading cell surface molecules .
(A) Trypsins; (B) Subtilisins; (C) GH18 chitinases; (D) Cytochrome P450s. Black branches identify orthologous loci in M. anisopliae and M. acridum. Red and green branches identify genes that are only present in M. anisopliae or M. acridum, respectively. Lineage specific genes expressed by these species on cockroach cuticle are marked with a blue asterisk. Lineage specific genes expressed on locust cuticle are marked with a purple circle. Protease family classification refers to Table S7 for trypsins and Table S8 for subtilisins.
Many plant pathogens need glycoside hydrolases, pectate lyases and cutinases to degrade the plant cuticle (waxy layer) and cell wall. The number of glycoside hydrolases (GH) possessed by M. anisopliae (156) and M. acridum (140) is close to the average for plant pathogenic fungi (150) (Table S10). However, only ∼20% of the Metarhizium GH genes (36 in MAA and 29 in MAC) were similar to PHI catalogued sequences as compared to 44% (70 genes) in F. graminearum and 29% (57 genes) in M. oryzae (Table S6). The plant pathogens in particular have additional GH3 cellulases while Metarhizium spp. lack the GH11 family of xylanases. GH3 and GH11 family genes are catalogued in the PHI-base. Overall, fewer genes were associated with plant utilization in Metarhizium than in plant pathogens. This included fewer putative cutinases (2 in Metarhizium spp. 8 to 18 in plant pathogens) and pectate lyases (7 in Metarhizium spp.; 9 to 25 in plant pathogens). However, the GH16 family of xyloglucan/xyloglucosyl transferases involved in decomposition of plant cell walls is well represented in the Metarhizium genomes (18 in MAA and 16 in MAC; 6 to 16 in plant pathogens) (Table S10). More predictably, GH18 chitinases involved in the digestion of insect cuticle chitin , are over represented in Metarhizium (30 in MAA and 21 in MAC; 5 to 14 in plant pathogens) (Figure 5C, Table S6). The few chitinases included in the PHI database are involved in fungal developmental processes, as chitin is not a substrate found in animal and plant hosts.
The number of genes for secreted lipases (12 in MAA, 5 in MAC) is well above the average found in other fungi, and 9 M. anisopliae and 5 M. acridum lipases showed significant similarity to genes in the PHI-base, as compared to 3 lipases each in F. graminearum and M. oryzae (Table S6). The role of individual Metarhizium lipases in pathogenicity has not been demonstrated, although a lipase activity inhibitor blocks infection processes in M. anisopliae . Lipases MAA_03127 and MAC_09232 showed best-hit relationships with an extracellular lipase FGL1 (PHI acc: 432) that is a virulence factor in F. graminearum .
Gene families for transportation and detoxification of compounds
Metarhizium genomes encode a large number of transporters (484 in MAA and 441 in MAC) (Table S11). Most transporters belong to the major facilitator superfamily (MFS) (269 in MAA; 236 in MAC) but the ATP-binding cassette (ABC) is also well represented (56 in MAA; 51 in MAC) (Table S11). Most of the ABC transporters (52/56 in MAA and 46/51 in MAC) and many of the MFS transporters (124/269 in MAA and 96/236 in MAC) were similar to genes catalogued in the PHI database (Table S6). The ABC transporters are usually implicated in defending the pathogen from host-produced secondary metabolites, whereas MFS proteins are typically involved in the transport of a wide range of substrates and may function as nutrient sensors . Interestingly, both Metarhizium species have more amino acid and peptide transporters than do other fungi (60 in MAA and 57 in MAC; 29 to 38 in other fungi), consistent with their being able to access a range of protein degradation products from insect sources. Homologs of these genes are absent from the PHI database. The only Metarhizium transporter with an experimentally determined function is the sucrose and galactoside transporter MRT (belonging to the MFS superfamily), which is required by M. anisopliae for rhizosphere competence but not for virulence . There are 6 MRT homologs in M. anisopliae and 5 in M. acridum but 12 in F. graminearum and 26 in M. oryzae, suggesting these genes could be generally important for establishing plant-fungus relationships.
Additional evidence about lifestyle could be found in the relatively large number of genes involved in detoxification in both Metarhizium genomes (Table 2, Table S2) as these potentially contribute to interactions with insect hosts (Table S6). However, families of dehydrogenases, acyl-CoA N-acetyltransferases, monooxygenases and cytochrome P450s (CYP) were preferentially expanded in M. anisopliae relative to M. acridum (Table 2, Table S5). One third of the dehydrogenases (92/271 in MAA and 80/236 in MAC) were putative PHI genes (Table S6). M. anisopliae was particularly enriched in zinc-containing alcohol dehydrogenases (17 in MAA; 7 in MAC) required for the biosynthesis of mannitol, a crucial factor for stress tolerance and virulence in the animal pathogen Cryptococcus neoformans . The monooxygenases in particular might be involved in rapid elimination of insect polyphenolics by ortho-hydroxylation of phenols to catechols .
The genome of M. anisopliae encodes 123 highly divergent CYP genes verses 100 CYPs in M. acridum (Figure 5D, Table S12). Ninety of the M. anisopliae CYPs and 69 of the M. acridum CYPs are similar to sequences in the PHI-base (Table S6). CYP genes are involved in oxygenation steps during alkane assimilation and the biosynthesis of secondary metabolites as well as with detoxification . M. anisopliae efficiently metabolizes the alkanes that are a major component of the surface layer of the insect cuticle (epicuticle) . Although the CYP52 subfamily is particularly important for alkane oxidation , M. anisopliae has only a single CYP52 (MAA_06634) compared to four in M. acridum (Table S12). However, MAA_06634 and its ortholog in M. acridum (MAC_09267) were highly expressed (see below) by M. anisopliae and M. acridum when infecting either cockroach or locust cuticles (Figure S5A). The other CYP genes up-regulated on cuticles were mostly involved in detoxification. M. anisopliae and M. acridum are predicted to contain four and two CYP504s, respectively. CYP504s are used by fungi to degrade phenylacetate , an antimicrobial compound found in plants and insects . The subfamily CYP53 is also represented in the PHI database as it is responsible for detoxification of benzoate and its derivatives . M. anisopliae and M. acridum have two and one CYP53 genes, respectively.
The subfamily CYP5081 involved in biosynthesis of helvolic acid, an antibiotic toxin , consists of four closely localized CYP loci (PHI genes) in M. anisopliae (MAA_06585, MAA_06586, MAA_06589 and MAA_06593) that are absent in M. acridum. All four CYP5081 genes were expressed by M. anisopliae infecting cuticles (Figure 5D). Both M. anisopliae and M. acridum have three CYP genes putatively encoding lipid dioxygenases (CYP6001: MAA_04954 and MAC_00208; CYP6002: MAC_05834; CYP6003: MAA_03481 and MAC_00918; CYP6004: MAA_0003) and two lipoxygenases (MAA_06278 and MAA_01260; MAC_01254 and MAC_9416). Oxylipins, the end products of these genes, allow Aspergillus nidulans to colonize plant seeds , and seeds are also a habitat for M. anisopliae , implying that a similar strategy is employed by Metarhizium to establish plant-fungus relationships.
Core genes for biosynthesis of secondary metabolites
M. anisopliae is a prolific producer of secondary metabolites including insecticidal destruxins , but with the exception of the serinocyclins  and NG-391 , the genes involved in their biosynthesis are unknown. However, diagnostic genes for secondary metabolite production include those encoding polyketides and non-ribosomal peptides (the most prominent classes of fungal secondary metabolites), as well as those responsible for modifications of the core moiety (a peptide or polyketide) such as genes encoding dehydrogenases, methyltransferases, acetyl transferases, prenyltransferases, oxireductases and CYPs . Consistent with expressed sequence tag studies , M. anisopliae appears to possess a much greater potential for the production of secondary metabolites than M. acridum or most other fungi (Tables S2 and S13). The M. anisopliae genome encodes 14 putative non-ribosomal peptide synthases (NRPS), 24 polyketide synthases (PKS) and 5 NRPS-PKS hybrid genes, which is more than M. acridum (13 NRPS genes, 13 PKS genes and 1 NRPS-PKS hybrid) and the average in other Ascomycetes (7 NRPS, 12 PKS genes and 1 NRPS-PKS) (Table S13). NRPSs and PKSs are strongly associated with pathogenicity in many plant pathogenic fungi and are well represented in the PHI database. As in other fungi, Metarhizium NRPS and PKS genes were often clustered together with genes that modify their products. One cluster suggests that Metarhizium might produce prenylated alkaloids (Figure S2). M. anisopliae possesses putative NRPS-like antibiotic synthetases (MAA_08272) consistent with defending the cadaver against microbial competitors. It also possesses a putative bassianolide synthetase (MAA_07513), a virulence factor of the insect pathogen Beauveria bassiana . The NRPS-like proteins MAA_07148 and MAC_06316 are most similar to ACE1, a PKS/NRPS hybrid that confers avirulence to M. grisea during rice infection . M. anisopliae NRPS MAA_00969 is similar (43% identity) to HTS1, the key enzyme responsible for the biosynthesis of the host-selective HC-toxin that confers the specificity of Cochliobolus carbonum to maize . Sixteen out of 24 PKS and 5/14 NRPS genes in M. anisopliae are species specific versus 4/13 PKS and 5/13 NRPS in M. acridum, suggesting lineage specific expansion of these families in both Metarhizium species. However, it is reassuring for present and future commercialization of these fungi that we found no orthologs of genes for the biosynthesis of the human mycotoxins gliotoxin and aflatoxin.
To recognize and adapt to invertebrate environments such as the insect cuticle, hemolymph and cadaver, Metarhizium spp. need to rapidly respond to changes in nutrient availability, osmolarity and the host immune system , . In Magnaporthe, the Pth11-like G-protein coupled receptor (GPCR) is a PHI gene (PHI-base acc: 404) because it mediates cell responses to inductive cues . M. anisopliae and M. acridum have 54 and 40 putative PTH11-like GPCRs, respectively compared to an average of 32 in other fungi (Table S2, Table S14). The Metarhizium sequences could be grouped into six subfamilies (Figure S3). G protein alpha subunits have been extensively studied in fungi and many are required for pathogenicity because they transduce extracellular signals leading to infection-specific development . Distinct roles for three G protein alpha subunit genes have been revealed in M. grisea, A. nidulans and N. crassa. A fourth G-alpha protein has been identified in the plant pathogens Stagonospora nodorum (SNOG_06158) , Ustilago maydis (UM05385) , and the saprophyte A. oryzae (BAE63877) . Each of the Metarhizium genomes also contain four G-alpha genes. The genes MAA_03488 and MAC_04984 show best hits (>30% similarity) with SNOG_06158, UM05383 and BAE63877, suggesting they may be orthologous. SNOG_06158 is the most highly up-regulated S. nodorum G-alpha gene in planta . Likewise, MAA_03488 and MAC_04984 are the most highly expressed G-alpha genes during infection of either cockroach or locust cuticles (see below, Table S20).
The chief mechanism used by bacteria for sensing their environment is based on two conserved proteins: a sensor histidine kinase (HK) and an effector response regulator (RR) that functions as a molecular switch controlling diverse activities. In fungi, two component pathways mediate environmental stress responses and hyphal development . M. anisopliae and M. acridum have 10 and 9 histidine kinases, respectively compared to 3 to 20 in other fungi (Table S2).
To regulate cell function, M. acridum has 192 protein kinases as compared to 161 in M. anisopliae which is still above the average (143) found in other fungi (Tables S5 and S15). Much of the M. acridum expansion involves cyclin dependent and cell division control kinases, suggesting that M. acridum has a particularly complex signal transduction cascade controlling cell division. As signal transduction is a critical part of fungal development and infection processes, and accordingly most of the kinases had orthologs in the PHI database (124/161 in MAA and 137/192 in MAC). The high frequency of pseudogenes among kinases (M. acridum, 1:6; M. anisopliae, 1:8), compared to transporters (M. anisopliae, 1:82; M. acridum, 1:33) and other gene families suggests that protein kinases have a particularly high rate of turnover (Table S16). Differentially lost genes tend to function in accessory roles so these kinases might have had redundant functions in signal transduction that changed rapidly under strong selective constraints.
Following signaling transduction, physiological responses are regulated by activation of different transcription factors. M. anisopliae has 510 putative transcription factors compared to 439 in M. acridum, the difference being largely due to M. anisopliae having more C2H2 zinc finger and Zn2/Cys6 transcription factors (Tables S5 and S17). These families are also expanded in some Aspergilli, where the characterized examples are involved in regulating diverse aspects of primary and secondary metabolism, including protein and polysaccharide degradation . The cAMP response element-binding (CREB) protein, a basic leucine zipper transcription factor (bZIP), is a major downstream transcription factor for cAMP/PKA pathways in mammals . CREB has not been characterized in fungi; however, our transcriptome data shows that a putative bZIP transcription factor (MAA_02048 or MAC_02758) is highly expressed by each Metarhizium species coincident with up-regulation of protein kinase A (see below). The physiological role(s) of MAA_02048 are currently under investigation.
Comparative transcriptome analysis
Insect bioassays confirmed that M. acridum killed locusts but not cockroaches, while M. anisopliae killed both insects (Figure S4). In order to identify the putative signal transduction and metabolic pathways involved in formation of infection structures, we used RNA-Seq to compare transcriptional responses of M. anisopliae and M. acridum to infection of the optically clear hind wings of adult locusts and cockroaches, respectively. A time period of 24 hours was chosen to focus on the crucial processes involved in prepenetration growth e.g., adhesion to epicuticle, germination and production of appressoria .
After sequencing >2.5 million tags for each treatment, it was calculated that >82% of predicted M. anisopliae genes and >88% of predicted M. acridum genes were expressed during pre-penetration growth. This included more than 80% of the M. anisopliae and M. acridum genes with sequences similar to those in the PHI database (Table S18). Germination and growth by M. anisopliae and M. acridum on either insect triggered high level expression of genes associated with translation (e.g., ribosomal proteins) and post-translational modifications (e.g., heat shock proteins) (Figure S5, Table S19). However, otherwise, the two fungi differed greatly from each other in their transcriptional responses to each cuticle, and to a lesser extent the two cuticles elicited different responses from each fungus (Figure 6, Figure S6). The orthologs of many differentially expressed genes are involved in appressorial formation and function in plant pathogens (Table S19), including Cas1 from Glomerella cingulata and Mas1 from M. oryzae . Three of these genes were among the five most highly expressed M. acridum genes on locust cuticle. Their expression levels were ∼2-fold lower on cockroach cuticle, similar to a previously characterized cuticle binding adhesin, Mad1 . This is also consistent with a previous study which showed that M. acridum up-regulated (∼3-fold) a single Mas1-like gene (MAC_03649) in the extracts of locust cuticular lipids but this gene was down-regulated in extracts from beetles (∼4-fold) or cicadas (∼2-fold) .
Genes differentially expressed by M. anisopliae (A) and M. acridum (B) infecting cockroach versus locust cuticles. Genes differentially expressed by M. anisopliae versus M. acridum on cockroach (C) and locust (D) cuticles. The figures in parentheses are the number of genes significantly up- or down-regulated by each fungus.
Formation of appressoria would be expected to involve significant modifications of the germ tube cell wall. Between 6 to 10% of the genes highly expressed by M. anisopliae and M. acridum on host cuticles encoded cell wall proteins. However, cell wall remodeling may be a greater feature of post penetration development because a microarray study showed that ∼20% of insect hemolymph-induced genes were involved in cell wall formation . Evidently, different subsets of genes are required before and after penetration of the cuticle. Suppression-subtractive hybridization identified 200 genes expressed by M. acridum in the hemolymph of locusts , and only eight of these genes involved in translation were among the 100 genes that were most highly expressed by pre-penetration germlings.
About 60% of the transcripts expressed by M. anisopliae in liquid cultures containing insect cuticles encoded secreted products, including many proteases , as compared to ∼20% of the transcripts in pre-penetration germlings (Table S19), indicating that growth in culture does not mimic the environment experienced on the insect surfaces. Despite the lineage-specific expansion of protease gene families in Metarhizium spp., only a few proteases were abundantly expressed by either species on insect epicuticles. Two trypsins were highly expressed by M. anisopliae on cuticle surfaces, but similar to most subtilisins, they were not expressed by M. acridum. Early expression of proteases triggered by nitrogen starvation may allow M. anisopliae to sample the cuticle, resulting in further induction of proteases that could digest the proteinaceous procuticular layer . Consistent with this hypothesis, both Metarhizium species expressed several genes involved in recognition of nitrogen starvation signals, including MAA_03429 and MAC_02501, which resemble the STM1-like GPCR responsible for triggering adaptation to nitrogen starvation in fission yeast Schizosaccharomyces pombe  (Table S20).
The profile of dehydrogenases produced on insect cuticles was used to highlight metabolic pathways that participate in pre-penetration growth. The expression profile of dehydrogenases produced on locust and cockroach cuticles was highly correlated (r = 0.96) in M. anisopliae, but much less so in M. acridum (r = 0.69). The most abundant dehydrogenase transcripts expressed by M. anisopliae on both cuticles included enzymes involved in glycolysis, the citric acid cycle and the oxidative branch of the pentose phosphate pathway. Genes involved in metabolizing intracellular lipids, proteins and amino acids were also highly expressed, showing that lipids are an important nutrient reserve, and that there is a high turnover of proteins during the formation of appressoria as previously suggested for M. oryzae .
Similar to previous observations , M. acridum germlings only produce appressoria on locust cuticle, and these visually resemble the appressoria produced by M. anisopliae on both insect cuticles (Figure 7). Consistent with early host recognition events being key to establishing specificity, M. acridum but not M. anisopliae transcribed different Pth11-like GPCR genes on locust and cockroach cuticles (Figure S3A). The up-regulation of G-protein alpha subunit, phosphatidyl inositol-specific phospholipase C, protein kinase C, Ca/calmodulin-dependent kinase and extracellular signal-regulated protein kinases indicate that the mitogen-activated protein kinase pathway was strongly activated by M. anisopliae during infection of both insects and by M. acridum infecting locust cuticle. Unexpectedly, M. acridum expressed adenylate cyclase and protein kinase A at higher levels on cockroach cuticle even though appressoria formation was not induced (Figure 7, Table S20). Most of the up-regulated signal-tranduction genes were similar to known PHI genes that regulate infection processes in other fungi (Table S6). Overall, our results suggest that M. anisopliae and M. acridum are able to differentiate diverse host-related stimuli on locust and cockroach cuticles using distinct or shared signaling pathways involving PTH11-like GPCRs, calcium-dependant pathways and MAP kinases that are probably under subtle and sophisticated cross-pathway controls.
Both the MAP kinase and cAMP dependent protein kinase A (PKA) pathways were activated by M. anisopliae and M. acridum infecting cockroach and locust cuticles. PLC, phosphatidyl inositol-specific phospholipase C; PIP2, phosphatidylinositol 4,5-bisphosphate; IP3, inositol 1,4,5-triphosphate; DAG, diacylglycerol; PKC, protein kinase C; CaMK, calcium/calmodulin regulated kinase; ERK, extracellular signal-regulated protein kinase; CREB, a basic leucine zipper transcription factor that is a potential cAMP response element-binding protein; CO, conidium; AP, appressorium. Thicker arrows indicate pathways that are more highly expressed by M. acridum on either locust or cockroach cuticles.
Recent improvements in next generation sequencing technology and bioinformatics now allows the de novo assembly of high quality eukaryotic genomes , . We used such an approach to provide the first draft sequences of insect fungal pathogens M. anisopliae and M. acridum. Metarhizium species are the best studied insect pathogenic fungi and thus serve as an excellent starting point for gaining a broad perspective of issues in insect pathology. Sequencing two related species that evolved very different life styles provides a powerful method to derive lists of candidate genes controlling pathogenicity, host specificity and alternative saprophytic life styles. By using the experimentally verified pathogen-host interaction (PHI) gene reference database , we found that >16% of the genes encoded by each genome have significant similarities with genes involved in pathogenicity in other fungi, oomycetes or bacteria. Our study also highlighted secreted proteins which are markedly more numerous in Metarhizium spp. than in plant pathogens and non-pathogens, pointing to a greater complexity and subtlety in the interactions between insect pathogens and their environments. High resolution RNA-Seq transcriptomic analyses found that the two Metarhizium spp. have highly complicated finely-tuned molecular mechanisms for regulating cell differentiations in response to different insect hosts. These were the first large scale transcriptome studies done with insect pathogenic fungi grown under simulated insect parasitism rather than in liquid cultures.
Whole genome analyses indicated that Metarhizium spp. are closer to plant endophytes and plant pathogens than they are to animal pathogens like A. fumigatus and C. albicans. The finding suggested that Metarhizum may have evolved from fungi adapted to grow on plants even though they now infect insects. This inference is supported by the consistent existence of genes for plant degrading enzymes within Metarhizium genomes (Table S2). In contrast, fungal pathogens of humans that are seldom recovered from soil, such as Coccidioides, exhibit few of these enzymes or none . Even necrophytes such as Trichoderma reesei lack many families of plant cell wall degrading enzymes , and the existence of such families in Metarhizium spp. implies that these species are able to utilize living plant tissues. Potentially, these enzymes could also facilitate colonization of root surfaces but this must remain speculative because the genetic basis for rhizosphere competence is largely obscure in fungi , . Our identification of the full repertoire of Metarhizium genes will help to identify genes responsible for life on the plant root.
M. acridum contains fewer transposase genes than M. anisopliae which might be due to differences in repeat-induced point mutation (RIP). Both M. anisopliae and M. acridum have orthologs (MAA_03836 and MAC_00922) of the N. crassa RIP defective gene (E≤10−80), the only gene known to be required for RIP . The retention of this gene suggests that M. anisopliae might have undergone RIP at some stage in its evolution, even though its genome currently shows no bias towards C:G to T:A mutations. Creating new genes through duplication is almost impossible when RIP is very efficient , so the apparent loss of RIP in M. anisopliae may have been a compromise for the massive expansion of some gene families, though at the cost to M. anisopliae of increased transposition. M. acridum has a strong RIP bias, but RIP is only functional when meiosis is frequent. Cordyceps taii has been described as the sexual type (teleomorph) of Metarhizium taii ,  but the sexual stages of M. acridum (and M. anisopliae) are unknown. However, both Metarhizium species have a complement of apparently functional genes whose orthologs in N. crassa and A. nidulans are known to be required for meiosis and sexual development (Table S21). These include putative α-mating type genes and genes with similarity to a high mobility group (HMG) mating type gene, suggesting that they may have the potential to be either self (homothallic) or non-self (heterothallic) fertile under favorable conditions . More studies are required to understand the importance of the RIP mechanisms in the evolution of Metarhizium genomes and to determine the frequency of meiosis. Discovering whether M. anisopliae and M. acridum undergo sexual reproduction also has important implications for understanding the evolution of new strains of these pathogens.
Alternatively to an undiscovered sexual stage, the conservation of sex genes in an asexual species could be due to a recent loss of sexuality, pleiotropy or parasexual recombination following heterokaryon formation . The well known parasexual cycle that occurs in some fungi including Metarhizium provides another mechanism for hybridization . As with sexual hybridization there are numerous barriers between vegetative fusions of different fungal species with the major one being vegetative incompatability, which results from heterokaryon incompatability proteins that block exchange of DNA . M. acridum has fewer (25 genes) heterokaryon incompatibility proteins than M. anisopliae (35 genes), which suggests that M. acridum may be less reproductively isolated than M. anisopliae. However, it is likely that M. acridum with its more specialized lifestyle and narrow environmental range encounters fewer genetically distinct individuals than the more opportunistic M. anisopliae (Table S2).
The evolutionary transition of Metarhizium spp. to insect pathogenicity must have involved adaptations to insect-based nutrition, as indicated by the large number of proteases, lipases and chitinases that can digest insect cuticles and the host body (Table S6). Except for the lipid outer epicuticle, most of the barriers and nutritional resources in insects are proteinaceous, and Metarhizium has a full set of proteases including many different subtilisins, trypsins, chymotrypsins, metalloproteases, aspartyl proteases, cysteine proteases and exo-acting peptidases. The chymotrypsins are M. anisopliae specific, and may have been acquired by a horizontal gene transfer event . Otherwise, the ∼2–10-fold expanded repertoire of various families of secreted proteases has been the result of preservation by natural selection of duplicated genes. These may have facilitated the adaptation to heterogenous environments. Thus, the abundance of aspartyl proteases and carboxypeptidases (active at low pH) and subtilisins and trypsins (active at high pH), reflects the ability of M. anisopliae to grow in media with a wide range of pH values . The ability to produce large quantities of secreted proteases will obviously assist in the rapid degradation of insect host barriers, but the diversity of different proteases might also have been selected because insects frequently exploit anti-fungal protease inhibitors .
With the exception of the trypsins, most of the proteases with orthologs in the PHI-base (Table S6) are reported to have a major function in degrading host barriers. Fungal trypsins are regarded as markers of pathogenicity as they are almost exclusively found in pathogens of plants, animals or fungi . M. anisopliae has more trypsins than any other sequenced fungus, including M. acridum, which indicates a recent evolution of this gene family by gene duplication in M. anisopliae (Figure 1C). We also infer that differential gene loss has occurred due to the existence of six trypsin pseudogenes in M. anisopliae (Table S16). At least two active trypsins are expressed during insect infection , but the role of these trypsins in disease has not been demonstrated. The only sequences similar (E<1×10−10) to Metarhizium trypsins in the PHI database are from the oomycete plant pathogen Phytophthora sojae (PHI acc.: 652 and 653). Plants produce diverse glucanases to degrade pathogen cell walls, and the P. sojae trypsins quench this by degrading the glucanases . It is feasible that a similar strategy could occur in insect-fungus interactions since the β-glucan recognition proteins, β-1,3-glucanases and β-1,4-glucanases involved in insect immune responses are similar to the anti-fungal glucanases produced by plants .
To date ∼20 Metarhizium genes that contribute to infectious capacity have been described . These have provided important new insights into the novel mechanisms by which pathogens evade host immunity by masking cell wall components with a collagen , differentially attach to insects or plants with different adhesins  and regulate intracellular lipid stores with a perilipin . Some of these genes, like the collagen MCL1 (MAA_01665), seem to be specifically associated with pathogenicity in M. anisopliae, showing that analysis of orphan (species-specific) genes will be crucial for a full understanding of pathogenicity. Other genes and gene families are generally associated with pathogenicity and can be predicted with the help of the PHI database. The >370 families of genes categorized as containing PHI genes in Metarhizium therefore represent good leads for dissecting the molecular genetics of pathogenicity. Many families like the crotonases involved in fatty acid metabolism , the PacC transcription factor that mediates the environmental pH signal, and the suppressers of defense responses such as catalases and superoxide dismutases have been well documented as virulence factors in diverse pathogens of plants and animals . It would be surprising if they were not involved in Metarhizium infection processes.
Other sequences identified from comparisons with the PHI database may be less generic in their impact on pathogenesis. As well as secreted proteins, the interaction between a pathogen and its host is to a large extent orchestrated by the proteins that are localized to the cell wall or cell membrane, and these categories are well represented in the PHI database. Plant and animal pathogens frequently have a subset of extracellular membrane proteins containing an eight-cysteine domain referred to as CFEM. In plant pathogens, CFEM-containing proteins function as cell-surface receptors or signal transducers, or as adhesion molecules in host-pathogen interactions . Deletion of CFEM-containing proteins produces a cascade of pleiotropic effects in C. albicans, most effecting cell-surface-related properties including the ability to form biofilms . The genomic sequences reveal that Metarhizium species also have CFEM-containing proteins (MAA_03310, MAA_04981 and MAA_07591 in M. anisopliae; MAC_09015 and MAC_09359 in M. acridum), and functional analysis is underway to investigate the role they play in M. anisopliae development and pathogenesis. There are many other putative PHI protein families that need to be verified as virulence or pathogenicity determinants in Metarhizium. For example, CheY-like domain proteins are response regulators in some bacterial two-component signaling systems , but their roles in fungi remain to be determined. Metarhizium spp. have an average number of histidine kinases compared to other filamentous fungi, and yet M. anisopliae, M. acridum, F. graminearum and M. oryzae have 4, 3, 2 and 0 CheY-like proteins, respectively (Table S6), indicating that M. anisopliae is comparatively well supplied with putative effector proteins that promote responses to stimuli. Much more unexpectedly, M. anisopliae has 6 (M. acridum has 1) homologs of heat-labile enterotoxins that play important roles in bacterial pathogenesis . The HMG proteins involved in fungal sexuality are also required for fungal pathogenicity . Both Metarhizium species have four HMG proteins that are predicted to be PHI genes (Table S6).
M. anisopliae produces large numbers of proteins and secondary metabolites that might be dedicated to host interaction and countering insect defenses . The identity and molecular functions of most secondary metabolite encoding genes remain to be determined in Metarhizium, and it will be intriguing to investigate which of their products are required for pathogenicity and or host specificity. However, with respect to the number of PKS and NRPS genes, M. anisopliae appears to possess a greater potential for the production of secondary metabolites than M. acridum and other sequenced Ascomycetes. M. anisopliae kills hosts quickly via toxins and grows saprophytically in the cadaver. In contrast, M. acridum causes a systemic infection of host tissues before the host dies which suggests limited production of toxins, or none . The presence of NRPS MAA_00969 in M. anisopliae is remarkable as almost all similar genes encoding host selective toxins were found in the Dothideomycetes . It is unlikely that MAA_00969 and HTST1 (encoding the HC-toxin) evolved independently, and one possibility is that MAA_00969 was acquired by an interspecific horizontal gene transfer event. There is no evidence to date that M. anisopliae has a relationship with any plant species that would require a specific toxin, and there are no reports of host-specific toxins in fungal pathogenesis of animals or insects.
Specialization in host range in various Metarhizium lineages is associated with a reduction in the range of molecules the fungi can utilize for nutrition or are able to detoxify . Consistent with this is the deficit of dehydrogenases (DHGs) in M. acridum relative to M. anisopliae or saprophytic fungi (Table 2). M. anisopliae also has more cytochrome P450s (CYPs), which are used by fungi to detoxify diverse substrates . Thus, the additional CYPs and DHGs encoded by M. anisopliae may enable it to detoxify the toxic repertoires of multiple insect hosts, as compared to M. acridum that infects only locusts. CYPS and DHGs also contribute to production of different secondary metabolites by oxidation (CYPs) and dehydroxylation (DHGs) of the backbone structures produced by the PKSs and NRPSs . M. anisopliae's PKS and NRPS clusters contain 18 CYPs and 21 DHGs, while M. acridum's PKS and NRPS clusters contain 3 CYPs and 12 DHGs. The insecticidal destruxin A–F subclasses produced by M. anisopliae have the same backbone structure, but more than 30 different analogues . These analogs presumably derive principally from the action of CYPs or DHGs, but the molecular mechanisms have not been determined.
Comparative global transcriptional studies of M. anisopliae and M. acridum provided a broad-based analysis of gene expression during early colonization processes, particularly in terms of the genes involved in host recognition, metabolic pathways and pathogen differentiation (Figure 7, Figure S6). About 20% of the genes most highly expressed by both Metarhizium species are putative PHI genes (Table S19). In spite of the abundance of protease genes in the Metarhizium genomes only a few proteases, mostly the trypins were expressed in the early stages of infection. As mentioned above, the trypsins could possibly serve as suppressors of host defenses. Studies in a range of plant pathogens suggest that early infection is characterized by the catabolism of internal lipid stores and that polymerized substrates are used after the readily available substrates are exhausted , . The transcriptome of M. anisopliae shows that it also uses internal lipid stores early in infection, which is consistent with a previous study . Proteases and chitinases are secreted later at very high levels to digest the protein-chitin procuticles . The occurrence of a stress condition during the early phase of the interaction with the insect host was indicated by the massive up-regulation of heat shock proteins (HSPs). MAA_04726 and MAC_01954 are similar (E<1×10−160) to an HSP90 from C. albicans that is a crucial virulence factor governing cell drug resistance and morphogenetic transition . The other highly expressed Metarhizium HSPs (e.g., HSP30s and HSP70s) are considered to be part of a general defense response and did not resemble sequences in the PHI database.
In spite of differences in infection procedures, we were able to identify some concordance between up-regulated Metarhizium genes and metabolic networks up-regulated by M. oryzae  and the mycoparasite Trichoderma harzianum . In particular, during early host colonization, they all up-regulated pathways associated with translation, post-translational modification, and amino acid and lipid metabolism. Metarhizium spp. also resemble M. oryzae and T. harzianum in that pathogenicity is associated with nitrogen deprivation and related stresses, indicating that at least some of the physiological conditions on insects, plants and fungal hosts might be similar. For example, the S. pombe STM1 gene links environmental nitrogen with cell differentiation . The up-regulation of similar STM1-like receptors by the three pathogenic fungi could be a common mechanism for linking low levels of nitrogen on the host surfaces with differentiation of infection structures.
In spite of their different host ranges, developmental processes within M. anisopliae and M. acridum are very similar, e.g. formation of appressoria and blastospores. However, comparatively analyzing their host-invading transcriptomes suggested that recognition might be determined in part by regulatory controls that exclusively limit expression of genes for pathogenicity-related developmental processes to individual hosts. Functional characterization should elucidate whether the expansion in M. anisopliae of several families of signal receptors and response elements is indicative of functional redundancy and/or reflective of a need for fine-tuned sensing of the host environments. The differentially regulated Pth11 GPCR genes are clear early candidates for further functional analysis to confirm their role as regulators of pathogenicity, and to investigate how their function varies between strains with different host ranges. Such studies could define the core set of host-specific transcripts and identify targets for effectively altering host range.
In conclusion, we have identified significant differences in gene contents and transcriptional regulations between M. acridum and M. anisopliae, that have led to the latter having a wider biochemical repertoire available for infecting multiple hosts. The genomic sequences will facilitate identifying candidate genes for manipulation to increase the benefits of applying Metarhizium not just as an insecticide but also potentially as a biofertilizer. The range of exploitable fungal virulence genes is enormous as besides the putative PHI genes, other virulence factors such as the systems for evading host immunity are of particular interests.
Materials and Methods
M. anisopliae strain ARSEF 23 has been studied in the laboratory for more than 40 years . It is a generalist insect pathogen that successfully infects locusts, caterpillars, flies, crickets and beetles, amongst others, and is classified as a Group A strain (good germination in many media and production of appressoria against a hard hydrophobic surface in yeast extract medium) . M. acridum CQMa 102 can only infect acridids and is being mass produced for large-scale locust control in China . It is classified as a Group D strain (little or no germination in yeast extract or glucose media). A recent taxonomic revision assigns M. anisopliae ARSEF 23 to a new species, viz., M. robertsii .
Genome sequencing and assembly
The genomes of M. anisopliae and M. acridum were sequenced with the next generation sequencing technology Illumina. DNA libraries with 200 bp, 2 kb and 6 kb inserts were constructed and sequenced with the Illumina Genome Analyzer sequencing technique at the Beijing Genomics Institute at Shenzhen with protocols described previously for the giant panda genome . The genome sequences were assembled using SOAPdenovo . For syntenic relationship analysis, the scaffolds of both genomes were oriented by MEGABLAST for dot plotting and a pair-wise comparison with an Argo Genome Browser .
Annotations of the genomic sequences of M. anisopliae and M. acridum were performed with Augustus , specifically trained with >6000 unique sequenced Metarhizium ESTs, and the annotated information of F. graminearum was incorporated as a reference. An ab initio predictor, GeneMark  was additionally used for ORF prediction with both Metarhizium genomes. Thorough manual checks were conducted on parallel comparisons of the results from two prediction methods. All questionable ORFs were individually subjected to Blast searches against the NCBI curated refseq_protein database and the individual prediction with the best hit was selected for each ORF. Pseudogene identification was conducted with the pipeline of PseudoPipe . Transfer RNAs (tRNAs) were predicted with tRNAscan-SE  and ARAGORN . Secreted proteins were identified by SignaIP 3.0 analysis (http://www.cbs.dtu.dk/services/SignalP/).
Orthology and phylogenomic analysis
Ortholog conservation in fungi was characterized with Inparanoid 7.0 . In total, 946 orthologous proteins were acquired and aligned with Clustal X 2.0 . A maximum likelihood phylogenomic tree was created using the concatenated amino acid sequences with the program TREE-PUZZLE using the Dayhoff model . The divergence time between species was estimated with the Langley-Fitch method with r8s  by calibrating against the reassessed origin of the Ascomycota at 500–650 million years ago .
Protein family classification and evolution analysis
Whole genome protein families were classified by InterproScan analysis (http://www.ebi.ac.uk/interpro/) in combination with the Treefam methodology that defines a protein family as a group of genes descended from a common ancestor . To identify potential pathogenicity and virulence genes, whole genome blast searches were conducted against protein sequences in the pathogen-host interaction database (version 3.2, http://www.phi-base.org/) (E<1×10−5). The families of proteases were additionally classified by Blastp against the MEROPS peptidase database (http://merops.sanger.ac.uk/). Transporters were classified based on the Transport Classification Database (http://www.tcdb.org/tcdb/). The cytochrome P450s were named according to Dr. Nelson's P450 database (http://drnelson.utmem.edu/CytochromeP450.html). G-protein coupled receptors, protein kinases, transcription factors and GH families were classified by Blastp against GPCRDB (http://www.gpcr.org/7tm/), KinBase (http://kinase.com/), Fungal Transcription Factor Database (http://ftfd.snu.ac.kr/) and CAZy database (http://www.cazy.org/), respectively. All Metarhizium genes with significant hits (E value ≤ 10−5) to GPCRDB sequences and that contained 7 transmemebrane helices (analyzed with http://www.cbs.dtu.dk/services/TMHMM/) were included as putative GPCRs. To analyze fungal secondary metabolite pathways, the genome annotation data from both species were coordinated and analyzed with the program SMURF (http://www.jcvi.org/smurf/index.php). The evolution of protein family size variation (expansion or contraction) was analyzed using CAFE .
Repeat and repeat-induced point mutation (RIP) analysis
Genome repetitive elements were analyzed by Blast against the RepeatMasker library (open 3.2.8) (http://www.repeatmasker.org/) and with the Tandem Repeat Finder . RIP index was determined with the software RIPCAL by reference against the non-repetitive control families . The transposons/retrotransposons encoding transposases/retrotransposases were classified by Blastp analysis against the Repbase (http://girinst.org/).
The hind wings from locusts (Locusta migratoria) and cockroaches (Periplaneta americana) were collected and surface sterilized in 37% H2O2 (5 min), washed in sterile water twice and dipped in conidial suspensions (2×107 spores per ml) of M. anisopliae ARSEF 23 or M. acridum CQMa 102 for 20 seconds. The inoculated wings were placed on 1% water agar and incubated at 25°C for 24 hrs. The wings with fungal cells were homogenized in liquid nitrogen and the total RNA was extracted with a Qiagen RNeasy mini kit plus on-column treatment with DNase I. Messenger RNA was purified from 6 µg total RNA. After reverse transcription into double strand cDNA for tag preparation according to the massively parallel signature sequencing protocol , it was sequenced with an Illumina technique. We omitted tags from further analysis if only one copy was detected or it could be mapped to different transcripts . Other tags were mapped to the genome or annotated genes by allowing if they possessed no more than one nucleotide mismatch. The abundance of each tag was converted to transcripts per million for quantitative comparison between samples. We used the test of false discovery rate (FDR≤0.001) to estimate the level of differential gene expression by each species under different induction conditions .
Metarhizium anisoliae and M. acridum were tested for their ability to kill adult locusts Locusta migratoria and cockroaches Periplaneta americana. For these experiments, conidia from each species were applied topically by immersion of cold-immobilized insects into aqueous suspensions of 5×108 spores per ml. Each treatment was replicated three times with 15 insects per replicate and the experiments were repeated twice. Mortality was recorded every 12 hours and the lethal time values for 50% mortality (LT50) were estimated .
The Whole Genome Shotgun projects have been deposited at DDBJ/EMBL/GenBank under the accession ANDI00000000 for Metarhizium acridum and ANDJ00000000 for Metarhizium anisopliae, respectively. The RNA-seq data have been deposited at NCBI GEO repository with accession numbers GSM612996, GSM612997, GSM612998 and GSM612999 for the samples of M. anisopliae infection of locust, M. anisopliae infection of cockroach, M. acridum infection of locust and M. acridum infection of cockroach, respectively.
Comparative structural mapping of selected regions from Metarhizium anisopliae (MAA) and M. acridum (MAC) genomes. (A) An example of highly syntenic relationships between selected regions from MAA and MAC; (B) Examples of lineage specific regions from MAA and MAC containing high densities of species-specific genes, proteins with unknown functions and transposases; (C) Selected syntenic regions showing occurrence of subtilisin duplications in MAA with no counterpoints in MAC.
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Schematic comparison of a conserved gene cluster in Metarhizium anisopliae and M. acridum putatively involved in synthesis of secondary metabolites, including ergot alkaloids. The core genes dimethylallyl tryptophan synthase (DMAT, an aromatic prenyltransferase that carries out the first committed step of ergot alkaloid biosynthesis) and nonribosomal peptide synthetases (NRPS) were predicted by SMURF (http://www.jcvi.org/smurf/index.php). Ergot alkaloid proteins A and B are involved in forming the D ring of the ergot alkaloid structural framework. Dynamin GTPases are involved in endocytosis and secretion. Ergot alkaloids are toxins and important pharmaceuticals that are produced biotechnologically on an industrial scale.
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Characterization of predicted Pth11-like G-protein coupled receptors (GPCR) encoded in the Metarhizium anisopliae (MAA) and M. acridum (MAC) genomes. (A) Neighbor-joining phylogeny of GPCRs from MAA and MAC showing that they are clustered into six subfamilies. The arrows indicate that the GPCR gene most highly transcribed by MAA on either locust or cockroach cuticles is MAA_06268, whereas the GPCR genes most highly expressed by MAC on locust and cockroach cuticles are MAC_00494 and MAC_03366, respectively; (B) The number of GPCR genes in each subfamily in M. anisopliae and M. acridum.
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Bioassays of Metarhizium anisopliae and M. acridum against adult locusts (A) and cockroaches (B). The median lethal times (LT50) vs. locusts (Locusta migratoria) were 8.60±0.15 and 6.54±0.37 days for M. anisopliae and M. acridum, respectively. For cockroaches (Periplaneta americana), the LT50 for M. anisopliae was 10.10±0.37 days. M. acridum was non-pathogenic to cockroaches.
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Differential gene expression profiling for selected orthologous genes from Metarhizium anisopliae and M. acridum infecting locust and cockroach cuticles, respectively. (A) Cytochrome P450 genes; (B) Serine protease subtilisin genes; (C) Trypsin genes; (D) GH18 family of chitinase genes. The Heat Map figures were generated using the log2 ratio of corresponding MAA gene transcription per million (TPM) data against MAC TPM data, i.e. Log2(MAA_TPM/MAC_TPM). The figures show M. anisopliae genes that are up-regulated (red) and down-regulated (green) relative to M. acridum.
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Functional classification of differentially expressed genes. Number of genes expressed by M. anisopliae (A) and M. acridum (B) infecting cockroach (blue bars) and locust (red bars) cuticles. The number of genes differentially expressed by M. anisopliae (blue bars) and M. acridum (red bars) on locust (C) and cockroach (D) cuticles.
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The number of protein-coding genes and genome size of Metarhizium species and other Ascomycetes.
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Sizes of selected gene families in Metarhizium spp. and other Ascomycetes.
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Expression of putative transposase coding genes by Metarhizium anisopliae and M. acridum during infection of cockroach (CO) and locust (LO) cuticles.
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The number of pseudogenes (by class) in the Metarhizium anisopliae and M. acridum genomes.
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Expanded (highlighted in green) and contracted (highlighted in yellow) protein families in Metarhizium anisopliae and M. acridum.
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Major protein families of Metarhizium spp. and plant pathogenic fungi implicated in the pathogen-host interactions.
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Trypsin proteases encoded in Metarhizium anisopliae and M. acridum genomes, arranged by MEROPS family.
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Subtilisin proteases encoded in Metarhizium anisopliae and M. acridum genomes, arranged by MEROPS family.
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Aspartyl proteases encoded in Metarhizium anisopliae and M. acridum genomes, arranged by MEROPS family.
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Carbohydrate-degrading enzymes in Metarhizium species and other fungi, arranged by GH family.
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Identification of genes coding for membrane transporters in Metarhizium anisopliae and M. acridum.
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Cytochrome P450 (CYP) genes encoded in Metarhizium anisopliae and M. acridum genomes, arranged by CYP family.
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The number of core genes involved in the biosynthesis of secondary metabolites in different fungi.
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The number of G-protein coupled receptors encoded in Metarhizium anisopliae and M. acridum genomes, arranged by class.
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The number of genes coding for protein kinases in Metarhizium anisopliae and M. acridum, arranged by class.
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Classification of conserved domains retained in the pseudogenes of Metarhizium anisopliae and M. acridum.
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The number of genes coding for transcription factors in Metarhizium anisopliae and M. acridum, arranged by family.
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General features of the high throughput transcriptome analysis.
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The 100 most highly expressed genes in Metarhizium anisopliae and M. acridum infecting locust and cockroach cuticles.
(0.21 MB DOC)
Differential expression of genes involved in signal transduction.
(0.10 MB DOC)
The authors appreciate the help of Prof. David Nelson for cytochrome P450 classification and Dr. Jongsun Park for transcription factor analysis. We also acknowledge the sequencing and assembly help from Beijing Genomics Institute at Shenzhen, China.
Conceived and designed the experiments: RJ St. Leger, C Wang. Performed the experiments: Q Gao, SH Ying, Y Shang, Z Duan, W Huang, B Wang. Analyzed the data: Q Gao, K Jin, SH Ying, Y Zhang, G Xiao, Y Shang, Z Duan, X Hu, XQ Xie, G Zhou, G Peng, Z Luo, W Huang, B Wang, W Fang, S Wang, Y Zhong, LJ Ma, GP Zhao, Y Pei, MG Feng, Y Xia, C Wang. Contributed reagents/materials/analysis tools: Y Pei, MG Feng, Y Xia. Wrote the paper: RJ St. Leger, C Wang.
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