The humoral response to fungal and Gram-positive infections is regulated by the serpin-family inhibitor, Necrotic. Following immune-challenge, a proteolytic cascade is activated which signals through the Toll receptor. Toll activation results in a range of antibiotic peptides being synthesised in the fat-body and exported to the haemolymph. As with mammalian serpins, Necrotic turnover in Drosophila is rapid. This serpin is synthesised in the fat-body, but its site of degradation has been unclear. By “freezing” endocytosis with a temperature sensitive Dynamin mutation, we demonstrate that Necrotic is removed from the haemolymph in two groups of giant cells: the garland and pericardial athrocytes. Necrotic uptake responds rapidly to infection, being visibly increased after 30 mins and peaking at 6–8 hours. Co-localisation of anti-Nec with anti-AP50, Rab5, and Rab7 antibodies establishes that the serpin is processed through multi-vesicular bodies and delivered to the lysosome, where it co-localises with the ubiquitin-binding protein, HRS. Nec does not co-localise with Rab11, indicating that the serpin is not re-exported from athrocytes. Instead, mutations which block late endosome/lysosome fusion (dor, hk, and car) cause accumulation of Necrotic-positive endosomes, even in the absence of infection. Knockdown of the 6 Drosophila orthologues of the mammalian LDL receptor family with dsRNA identifies LpR1 as an enhancer of the immune response. Uptake of Necrotic from the haemolymph is blocked by a chromosomal deletion of LpR1. In conclusion, we identify the cells and the receptor molecule responsible for the uptake and degradation of the Necrotic serpin in Drosophila melanogaster. The scavenging of serpin/proteinase complexes may be a critical step in the regulation of proteolytic cascades.
Serpin inhibitors control a wide range of rapid physiological responses that are activated by proteolytic cascades, such as blood coagulation, inflammation, the complement pathway, and angiogenesis. They interact with their target proteinases by a “suicide inhibition” mechanism, which generates an inert, denatured, serpin/proteinase complex. In mammals, humoral serpins are secreted from the liver into the blood plasma. The denatured complex is later endocytosed back into the liver and degraded. In Drosophila, the Necrotic serpin is secreted from the fat-body into the haemolymph, where it controls the humoral immune response. We show here, however, that Necrotic is not endocytosed in the fat-body, but in the garland and pericardial athrocytes. These cells clear serpins from the haemolymph extremely rapidly. The Necrotic-binding receptor for this process is LpR1, a member of the LDLR family. The endocytosed serpin is targeted for lysosomal degradation, with none being recycled to the haemolymph. More importantly, we show that mutations in LpR1 cause a profound effect on the immune response. Thus, our results indicate that the scavenging of serpin/proteinase complexes might be a critical step in the regulation of proteolytic cascades.
Citation: Soukup SF, Culi J, Gubb D (2009) Uptake of the Necrotic Serpin in Drosophila melanogaster via the Lipophorin Receptor-1. PLoS Genet5(6): e1000532. https://doi.org/10.1371/journal.pgen.1000532
Editor: Eric Rulifson, University of California San Francisco, United States of America
Received: February 26, 2009; Accepted: May 22, 2009; Published: June 26, 2009
Copyright: © 2009 Soukup et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Spanish Ministry of Education and Science Grants to DG and JC. Additional support was provided from The Department of Industry, Tourism, and Trade of the Government of the Autonomous Community of the Basque Country (Etortek Research Programs) and from the Innovation Technology Department of Bizkaia County. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The immune response to pathogen challenge in humans consists of an immediate “innate” response (mediated via cellular responses and the action of antimicrobial peptides), followed by a delayed “acquired” response (mediated by antibodies). In insects, the antibody response is absent, but these organisms synthesise antibiotic peptides, activate macrophage-like cells and mount a melanization response . The necrotic (nec) gene encodes a proteinase inhibitor of the serpin family (serine proteinase inhibitor). Nec controls a proteolytic cascade which activates the innate immune response to fungal and Gram+ bacterial infections . The Nec serpin carries an N-terminal extension which modifies its substrate specificity and is cleaved following immune-challenge . In nec null mutants, the Toll-mediated immune response is constitutively activated, even in the absence of infection, implying that Nec continually restrains this immune response. The serpins have been extensively studied in mammals, where they regulate many extracellular proteolytic cascades. The coagulation, inflammatory and complement pathways are controlled by α1-Antithrombin, α1-Antitrypsin and C1-Inhibitor, respectively –; while Plasminogen Activator Inhibitor-1 modulates angiogenesis, affecting both wound-healing and tumour growth . Disorders in serpin metabolism underlie a major group of human genetic diseases, the serpinopathies, which are associated with failure to clear inert serpin polymers ,, and homologous mutations in Necrotic similarly form inactive polymers ,.
Serpins interact with their target proteinase via a “suicide-inhibition” mechanism, which destroys both serpin and proteinase and generates a covalently-linked complex . Inert serpin/proteinase complexes are removed from circulation by endocytosis in the liver , via receptors of the low-density lipoprotein (LDLR) family ,. The LDLR family consists of a diverse group of cell surface receptors  that is evolutionarily conserved . LDLR/ligand binding is pH-dependent, so that the complex dissociates in the low pH environment of the endosomal compartment, allowing LDLR to be recycled to the cell surface –.
During endocytosis, the internalization of receptor-bound proteins requires Dynamin function for the pinching-off of clathrin-coated vesicles . Endocytosed proteins are transported to various intracellular compartments, with the Rab family of Ras-related GTPases being critical for coordinating vesicle formation, transport and fusion with the target membrane . In particular, maturation of the early endosome coincides with the replacement of Rab5 by Rab7 and the accumulation of lumenal vesicles to form multivesicular bodies (MVB) . MVB correspond to a class of late endosome, which requires Hook and Fab1 for maturation . Following LDLR/ligand dissociation, transport of the free LDLR back to the plasma membrane is mediated via Rab11-positive, recycling endosomes. The contents of MVBs are delivered by direct fusion, either to lysosomes, or to the plasma membrane . A key component in protein sorting from late endosomes to lysosomes is ubiquitination. In this process, HRS (hepatocyte growth factor-regulated kinase substrate) binds ubiquitin and interacts with ubiquitinated cargos in the early endosome , while Fab1 encodes a phosphatidylinositol(3)-phosphate 5-kinase which acts downstream of HRS ,. In Drosophila, MVB fusion with the lysosome requires the function of the deep orange (dor) and carnation (car) subunits of the HOPS (homeotypic vacuole fusion and protein sorting) trafficking complex .
The garland cells represent an attractive model system to study endocytotic processes, since they are giant cells that are highly active in clathrin-mediated endocytosis. They form two rows of loosely-connected cells surrounding the oesophagus at the junction with the proventriculus, but little is known about their biological function –. Garland cells are bi-nucleated with a highly vacuolated structure featuring deep invaginations of the plasma membrane . The pericardial cells, on the other hand, are arranged in two rows of approximately 20 cells, on either side of the heart. Both cell types have a similar morphology and express the WD40 domain protein, Rudhira , and the dot-Gal4 enhancer trap , while their positioning around the proventriculus and dorsal vessel ensures good contact with flowing haemolymph. In contrast to the garland cells, pericardial cells are mono-nucleate; although both cell types are bi-nucleate in Hyalophora cecropia and Calliphora erythrocephala ,. These two cell types are homologous to athrocytes in other insects  and share a nephrin/podocin based filtration mechanism with the vertebrate kidney glomerulus . Instead of being passed to a nephric tubule, however, the ultrafiltrate is endocytosed from lacunae in the garland and pericardial cells ,,. There is no connection between these athrocytes and the Malpighian tubules, which regulate the concentration of plasma ions and metabolites in Insects. In this respect, the garland and pericardial cell clusters resemble more the vertebrate reticulo-endothelial cells , than the kidney glomerulus. The reticulo-endothelial system consists of groups of phagocytic cells that take up denatured proteins, bacterial and viral components –.
In this study, we investigate the role of LDL-family receptors in the Toll-mediated immune response of Drosophila by dsRNA-knockout. We have tested the role of the Lipophorin Receptors, LpR1 and LpR2, in Nec processing. We show that Nec is taken up in the garland and pericardial cells by LpR1, probably as a serpin/proteinase complex. Antibody staining against different proteins involved in clathrin-mediated endocytosis and intracellular trafficking establishes that Nec is sorted and degraded in the garland and pericardial athrocytes, but not recycled to the haemolymph. In general, the paucity of Rab11-positive vesicles indicates that these cells do not recycle endocytotic components, but are the site of protein and peptide degradation.
Necrotic expression pattern and activation by infection
Northern-blot analysis showed that nec is expressed at low levels in larvae and moderate/high levels in pupae and adults, in unchallenged flies . Here we use quantitative RT-PCR to quantify nec transcript levels with and without infection. We found no nec expression in embryos, moderate levels in larvae and adults, and higher levels in pupal stages. Six hours after infection with M. luteus, nec transcript was up-regulated 10 fold in larvae and 7 fold in adults, to reach similar levels (Figure S1). Nec expression was induced 4–6 fold after septic injury with Micrococcus luteus and 3.4 fold after fungal infection with Beauvaria bassiana in adult flies . Given these results, we analysed Nec uptake after infection in both larvae and adults.
In situ hybridization showed strong nec expression in the fat-body of infected wild-type larvae, which is lost in nec transcript null larvae (Figure S2). In addition, nec transcript was detected in mid- and hind-gut. We did not detect nec transcript in Malpighian tubules, imaginal discs, brain tissue, pericardial or garland cells (Figure S2) of wild-type larvae. These results are in broad agreement with expression array data from the FlyAtlas (http://flyatlas.org/atlas). The anti-Nec antibody detects protein staining in the fat-body of wild-type larvae, which is lost in protein null larvae (data not shown).
Nec is taken up from the haemolymph by the garland cells
We were initially unable to detect Nec protein in any larval or adult tissues, apart from the fat-body, using a rabbit polyclonal antibody that works on Western blots. Similar experiments using an antibody to Serpin27A also failed to detect antibody staining outside the fat-body. These preliminary findings are consistent with the hypothesis that serpin turnover in the haemolymph is rapid and that tissue-fixation might be slower than serpin degradation. To test this hypothesis, we blocked the formation of clathrin-coated vesicles, using the temperature-sensitive Dynamin mutation, shits1 . Third instar shits1 larvae were grown, infected and dissected at the permissive temperature of shits1 and shifted to the restrictive temperature during fixation. This procedure combines rapid fixation with a block in endocytosis.
Under these conditions, Nec antibody staining was absent, or barely detectable, in the garland cells of infected wild-type larvae, but strong staining was seen in the garland cells of infected shits1 larvae (Figure 1). The basal level of Nec expression was detected, at reduced intensity, in uninfected shits1 larvae (Figure 1D). Similar results were seen in pericardial cells and in adult stages. The specificity of the Nec antibody was confirmed by the loss of protein staining in a shits; nec null background (Figure S2D). Given that in situ hybridization failed to detect nec transcript (Figure S2C), the Nec protein in garland cells is presumably taken-up from the haemolymph. RNAi knockdown of nec transcript in the garland cells (of shits; UAS-necdsRNAi; dot-Gal4 UAS-GFP larvae) has no effect on Nec antibody staining, which remained strong (Figure S3). Note that garland cells bud-off endocytotic vesicles from labyrinthine channels, within a cortical region of several microns depth, rather than from the outer cell surface ,. As a result, blocking endocytosis leaves the walls of the labyrinthine channels coated with bound Nec protein.
(A) Wild-type garland cells (white arrow) 6 h post infection. (B) shits mutant 6 h post infection showing strong Nec localisation (red) to garland cells (white arrow). (C) Wild-type garland cells (white arrow) without infection. (D) shits mutant garland cells (white arrow) without infection show faint, basal Nec levels. The ring of garland cells is loosely inter-connected with fine Actin fibres, which frequently break during preparation. All preparations were fixed at 37°C. Nec (red), Actin (green), and DAPI (DNA) (blue).
To confirm that the Nec visualised in garland cells derives from protein synthesis in the fat-body we knocked down nec transcription in the fat-body, in shits; UAS-necdsRNAi/c564-Gal4 larvae. Garland cells from these larvae show no detectable Nec protein (Figure S4).
Taken together, these results indicate that garland and pericardial cells take-up Nec from the haemolymph and confirm that this protein is synthesised in the fat-body.
Time-course of Nec uptake in response to infection
The intensity of Nec-antibody staining in garland cells showed a rapid increase after infection with M. luteus, which is visible by 30 mins post-infection, peaks between 6 and 8 h and returns to basal levels within 24 h (Figure S5).
The Nec endocytotic pathway
Nec is endocytosed in clathrin-coated vesicles.
Clathrin-coated vesicles co-localised with Nec in garland and pericardial cells, in both larval and adult stages (Figure 2). Many vesicles stained AP50-positive (clathrin-coated) and Nec-positive; but some AP50− Nec+ and AP50+ Nec− vesicles were also seen. These results indicate that Nec is internalized in clathrin-coated vesicles and presumably re-sorted to other, clathrin-negative, compartments.
(A) larval garland cells, (A′) adult garland cells, (A″) larval pericardial cells and (A′″) adult pericardial cells merge of single channels showing co-localization of Nec and AP50 (yellow), Nec+ AP50− vesicles (red) and Nec− AP50+ vesicles (green). (B, B″) larval stage and (B′, B′″) adult stage Nec channel. (C, C″) larval stage and (C′, C′″) adult stage AP50 channel. (D, D″) larval stage and (D′,D′″) adult stage DAPI channel. Immunostainings were 6 hours post infection in a shits1 background. Nec (red), AP-50 (green), and DAPI (blue).
Nec is sorted through Rab-positive endosomes for degradation.
Rab5 antibody staining partially co-localizes with Nec-positive vesicles in early endosomes (Figure 3A). Driving mutant UAS-Rab5S43N expression in garland cells, under the control of dot-Gal4, resulted in strong accumulation of Nec-positive vesicles (Figure 3J) even in the absence of infection. Similarly, Nec partially co-localized with Rab7-positive late endosomes (Figure 3E) and expressing UAS-Rab7Q67 mutant protein lead to accumulation of Nec-positive vesicles in the absence of infection (Figure 3K). The UAS-Rab5S43N and UAS-Rab7Q67L transgenic constructs caused a similar block in Nec processing. Taken together, these results suggest that Rab5 and Rab7 are critical for Nec sorting and degradation in garland cells.
(A) Merge of B–D shows co-localization of Nec and Rab5 in yellow. (B) Nec channel. (C) Rab5 channel. (D) DNA channel. (E) Merge of B–D shows co-localization of Nec and Rab7 in yellow (F) Nec channel. (G) Rab7 channel. (H) DNA channel. (I) shits1 flies without infection. (J) UAS-Rab5S43N mutant flies without infection. (K) UAS-Rab7Q67L mutant flies without infection. Nec (red), Rab5 (green in A and B) Rab7 (green in E and G), and DAPI (blue).
Nec requires sorting through multi-vesicular bodies for degradation.
From the early endosome, the endocytotic cargo is sorted into multivesicular bodies and can be delivered to the lysosome for degradation. HRS antibody staining was used to identify ubiquitinated endosomes, in the sorting pathway from the early endosome  to multivesicular bodies. As shown in Figure 4A, Nec staining co-localizes with HRS staining in early endosomes.
(A) Merge shows co-localization of HRS and Nec in yellow. (B) Nec channel. (C) HRS channel. (D) DNA channel. Nec (red), HRS (green), and DAPI (blue).
To investigate lysosomal delivery, we used the dor, hook and car mutants of the HOPS complex to block late endosome/lysosome fusion ,,. In all three mutants, Nec-positive endosomes/MVB accumulate even in the absence of infection (Figure 5). We conclude therefore that Nec sorting through MVBs via the HOPS complex is required for lysosomal delivery.
(A) shits16h post infection. (B) shits1 without infection. (C) Wild-type 6 h post infection. (D) dor1/dor8 mutant without infection. (E) car1 mutant without infection. (F) hk1 mutant without infection. Nec (red), Actin (green), and DAPI (blue).
Nec co-localises with Fab1.
Fab1 acts downstream of HRS , and is required for vacuolar membrane trafficking and delivery to the lysosome. Antibody staining shows partial co-localization of Fab1 and Nec, indicating that Nec is destined for lysosomal degradation by Fab1 (Figure 6).
(A–D) Fab1 co-localizes with Nec in garland cells. (A) Merge shows co-localization of Lamp1 and Nec in yellow. (B) Nec channel. (C) Lamp1 channel. (D) DAPI channel. (A′–D′) Fab1 co-localizes with Nec in garland cells. (A′) Merge shows co-localization of Fab1 and Nec in yellow. (B′) Nec channel. (C′) Fab1 channel. (D′) DNA channel. Nec (red), Lamp1 (green in A and C), Fab1 (green in A′ and C′), and DAPI (blue).
Internalised Nec is targeted for lysosomal degradation and is not re-exported to the haemolymph.
An antibody to a lysosomal membrane protein, Lamp1, was used to confirm that Nec is trafficked to the lysosomal compartment. Co-localisation of Nec and Lamp1 antibody staining confirms that Nec is sorted from MVB to lysosomes, where it is degraded (Figure 6).
An alternative trafficking pathway for proteins within MVBs is that they can be re-exported to the haemolymph via Rab11-positive recycling vesicles . We found that garland cells contain few Rab11-positive vesicles in general, and that the Nec protein staining did not co-localize with those Rab11-positive vesicles that were present (Figure 7). Therefore, the Nec protein taken-up by the garland cells is targeted exclusively for lysosomal degradation.
(A) Merge shows distinct populations of Rab11-positive vesicles (green) and Nec-positive vesicles (red). (B) Nec channel. (C) Rab11 channel. (D) DAPI channel. Nec (red), Rab11 (green) and DAPI (blue) (E) larval wing discs with Rab11 and DAPI staining. (F) magnification of E.
Identification of the Nec trafficking receptor dsRNA knockdown assays.
The 6 Drosophila orthologues of the mammalian LDLR family were tested for an immune response function by dsRNA knockdown. Adult flies were injected with LDLR dsRNAs and 4 days later infected with M. luteus . Toll pathway activity was assayed by quantifying transcript levels of the antibiotic peptide Drosomycin (Drs), (Figure S6). Knockdown efficiencies for the D-LDLR orthologues were: LpR2 (92%), LpR1 (86%), CG33087 (87%), CG12139 (91%), arrow (56%) and CG8909 (88%), as estimated by qRT-PCR. Under these conditions, silencing of CG33087, CG12139, arrow and CG8909 does not affect Drs transcript levels, with a barely significant reduction with silencing of LpR2. Strikingly, LpR1-silencing doubles Drs transcript levels. This result implies that partial knockdown of LpR1 decreases Nec activity in the haemolymph. To confirm this result we measured nec and Drs transcript levels in Df(3R)lpr1, Df(3R)lpr2 and Df(3R)lpr1/2 in infected flies (Figure S7). Deficiency of LpR1 reduces nec (58%) and increases Drs transcript levels (206%), while deficiency of LpR2 has a weaker effect in the opposite direction. In the absence of immune challenge, Drs transcript levels are (1030%) higher in Df(3R)lpr1 than in wild-type flies (Figure S7).
Chromosomal deletion of LpR1 blocks Nec uptake into garland cells
The LpR1 and LpR2 genes are adjacent transcripts in Drosophila. Flies carrying either a chromosomal deletion for LpR1, (Df(3R)lpr1), the pBac(LpR1) mutation, or a deletion of both LpR transcripts (Df(3R)lpr1/2), fail to take up Nec into garland cells. Conversely, deletion of the LpR2 transcript only (in Df(3R)lpr2 flies) leaves Nec uptake unaffected (Figure 8).
(A–F) larval garland cells 6 h post infection (A) shits1 mutant. (B) Wild-type. (C) shits1; Df(3R)lpr1. (D) shits1; pBac(LpR1). (E) shits1; Df(3R)lpr2 (F) shits1; Df(3R)lpr1/2. All pictures captured with same setting and laser intensity. Nec (red), Actin (green), and DAPI (blue).
LpR1, LpR2, and Nec co-localize in garland and pericardial cells
Antibody staining of LpR1 and Nec in larval and adult garland and pericardial cells showed co-localisation (Figure 9). The equivalent experiments with LpR2 antibody showed that this receptor also co-localises with Nec (Figure S8). Taken together with the blocking of Nec uptake in Df(3R)lpr1 flies, these results confirm that LpR1 is the Nec trafficking receptor and that LpR2 is present, but does not traffic Nec, under these conditions.
(A–D) larval, (A′–D′) adult garland cells, (A″–D″) larval and (A′″–D′″) adult pericardial cells, 6 h post infection. (A, A′, A″, and A″′) Merge shows LpR1 and Nec co-localizing (yellow) in endosomes. (B, B′, B″, and B′″) Nec channel. (C, C′, C″, and C′″) LpR1 channel. (D, D′, D″, and D′″) DNA channel. Nec (red), LpR1 (green), and DAPI (blue).
The LpR1 receptor is required for efficient uptake of a Nec-proteinase complex
Digestion of the NecΔN core serpin (lacking the N-terminal peptide) with porcine pancreatic elastase (PPE) produces a covalently linked NecΔN-PPE complex with reduced levels of native NecΔN (Figure 10A). This native serpin+serpin/protease complex mixture was taken up more readily by garland cells than the pure NecΔN sample, provided that the LpR1 transcript was present. NecΔN-PPE complex uptake was blocked by the Df(3R)lpr1 and Df(3R)lpr1/2 chromosomal deletions. In addition, uptake of the undigested, native NecΔN, serpin was decreased by the Df(3R)lpr2 and Df(3R)lpr1/2 deletions (Figure 10G and 10J). These results are consistent with LpR1 being the receptor for the inert Nec-proteinase complex and LpR2 being the main receptor for native Nec, although other receptors may well be responsible for some of the native Nec binding.
(A) Formation of Nec-PPE complex. Coomassie blue stained PAGE gel showing PPE digest of NecΔN. The digestion reduces the intensity of NecΔN band to 20% and 8% of Nec is found in the complex band (arrow). The uptake of NecΔN-PPE complex uptake is blocked by the LpR1 and LpR1-2 deletions, but not the LpR2 deletion. Native Nec uptake is decreased by the LpR2 and LpR1-2 deletions. (B) shits1+PBS control; (C) shits1+NecΔN; (D) shits1+NecΔN/PPE complex. (E) shits1; Df(3R)lpr1+PBS control; (F) shits1; Df(3R)lpr1+NecΔN; (G) shits1; Df(3R)lpr1+NecΔN/PPE complex. (H) shits1; Df(3R)lpr2+PBS control; (I) shits1; Df(3R)lpr2+NecΔN; (J) shits1; Df(3R)lpr2+NecΔN/PPE complex. (K) shits1; Df(3R)lpr1/2+PBS control; (L) shits1; Df(3R)lpr1/2+NecΔN; (M) shits1; Df(3R)lpr1/2+NecΔN/PPE complex. All samples were prepared together and pictures captured with same confocal settings and laser intensity. Nec (red), HRP (cell membrane) (green), DAPI (blue).
The Necrotic serpin controls activation of the Toll-mediated immune-response in Drosophila, which represents the best-studied example of serpin-regulated proteolytic cascade in insects ,,. The Drosophila melanogaster genome encodes 15 putative inhibitory serpin transcripts that carry secretion-signal peptides . In addition to nec, the Spn27A serpin controls Toll-mediated morphogenesis in the embryo and the phenol-oxidase cascade in adults ,. Spn28D (CG7219) also regulates the phenol-oxidase cascade , while Spn77Ba regulates tracheal melanization, which also can trigger systemic expression of Drosomycin via the Toll pathway . In addition, the Spn42Da transcript inhibits furin, which is involved in the maturation of secreted proteins .
In mammals, serpins are removed from circulation by endocytosis and degraded in the liver as inactive serpin/proteinase complexes. This aspect of serpin metabolism, however, has not been studied previously in Drosophila. In this study, we identify the mechanism of serpin clearance by endocytosis in the garland and pericardial cells. These cells are known to take up ferritin  and GFP  and have been suggested to be homologous to mammalian reticulo-endothelial cells , or nephrocytes ,,. As in mammals, serpin turnover in Drosophila is extremely rapid, so that we were unable to detect the immune-response serpins Nec and Spn27A under normal conditions. However, freezing the pinching-off of endocytotic vesicles, using the shits1 mutation, allows us to detect serpin uptake. Endocytosed Nec is sorted, first to Rab5-positive early-endosomes and then to Rab7-positive late-endosomes. Disrupting these steps by expression of dominant negative UAS-Rab5S43 or UAS-Rab7Q67L, leads to accumulation of Nec-positive vesicles. Similarly, co-localisation of anti-Nec and anti-HRS antibody staining indicates that the serpin is present in early endosomes in the ubiquitin-dependent sorting pathway; while anti-Nec and anti-Fab1 confirms that the serpin is destined for lysosomal degradation. Blocking late-endosome/lysosome fusion using HOPS-complex mutants causes accumulation of Nec-positive endosomes/MVB, indicating that Nec sorting to MVB is required for lysosomal delivery. Co-localization of anti-Nec and anti-Lamp1 antibody staining confirms that Nec is delivered to lysosomes for degradation, while the absence of Nec staining in Rab11-positive vesicles indicates that none of the serpin is recycled from MVB to the haemolymph. In summary, Nec is cleared from the haemolymph and sorted through MVB, via the ubiquitin-dependent pathway, to lysosomes for degradation.
In vitro studies in mammals have shown that different members of LDLR family have different binding specificities to different native serpins and serpin/proteinase complexes ,. In this study, we have shown that LpR1 is the Nec trafficking receptor in vivo, but that neither LpR1 nor LpR2 traffics Spn27A. By analogy to mammalian systems, Nec is probably taken up by LpR1 as a complex with its target proteinase. In addition, pre-digestion of Nec with PPE increases Nec uptake in garland cells that are not deficient for the LpR1 receptor. Our results establish that active trafficking of Nec from the haemolymph can modulate the immune response. Nec clearance is extremely rapid, but deletion of the LpR1 gene sensitises the immune response: nec transcript levels decrease and Drs transcript levels increase. These results imply a regulatory feedback loop at the transcriptional level. In this context, it is significant that LpR1 appears to bind the non-inhibitory serpin/proteinase complex, in preference to the native Nec serpin. Clearance of the serpin/protease complex through the athrocytes appears to compete with a regulatory feedback loop affecting nec transcription.
In summary, we establish that the Nec serpin is taken-up via LpR1 from the haemolymph and degraded in the garland and pericardial athrocytes.
Materials and Methods
Fly strains and crosses
The following Drosophila stocks were obtained from the Bloomington Stock Centre and described in FlyBase: shits1, PBac(PB)LpR1c04916, hk1, dor8, dor1, car1, Df(2R)STI and nec2. The nec19 stock was described in  and Df(2R)pk-sple-51 in . The chromosomal deletion for nec was a trans heterozygous combination of Df(2R)pk-sple-51/Df(2R)STI, which completely lacks the nec and pk transcripts. UAS-Rab5S43 and UAS-Rab7Q67L are described in . We used two homozygous viable deletions that remove the individual LpR1 transcripts, Df(3R)lpr1 and Df(3R)lpr2, and a third homozygous viable deletion, Df(3R)lpr1/2, which removes both transcripts together, without including other genes (J. Culi unpublished data). The dot-Gal4 UAS-GFP stock is described in  and the fat-body Gal4 driver, c564-Gal4 was from Bloomington (GawB-c564). Stocks were grown on standard cornmeal/agar medium at 25°C, except those containing the shits1 mutation, which were grown at 18°C.
M. luteus infection
The Gram-positive bacterium Micrococcus luteus, strain CIP A270, was used to activate the Toll-mediated immune response. Adult flies were challenged by septic injury with a thin tungsten needle dipped into a concentrated M. luteus culture. Flies were collected after 6 h (for Nec assays) or 24 h (for Drs response). Larvae were infected by incubation on a Petri dish carrying an overnight culture of M. luteus.
Third instar larvae and adults were dissected in PBS at 20°C and fixed in 4% PFA solution at 37°C or at 4°C. Immunohistochemistry was performed according to . Non-specific background staining was reduced by adding BSA (1%) to the wash solutions. The following polyclonal guinea pig antibodies were used in this study: HRS at 1∶100 dilution , anti-Fab1 at 1∶200 dilution and anti-Lamp1 at 1∶500 dilution , LpR1 at 1∶100 dilution and LpR2 at 1∶100 dilution (J. Culi unpublished data). Polyclonal rabbit antibodies used were: anti-Nec at 1∶500 dilution , anti-Spn27 at 1∶500 dilution ; anti-Rab7 at 1∶2000 dilution , anti-Rab5 at 1∶50 dilution . Monoclonal mouse antibodies were purchased from BD Transduction laboratories∶anti-AP50 at 1∶25 dilution (to mark clathrin-coated vesicles)  and anti-Rab11 at 1∶100 dilution . Anti-HRP-Cy2 at 1∶100 dilution, to detect garland cell membranes (Jackson ImmunoResearch Laboratories). Alexa Fluor 488, Alexa Fluor 568 and Alexa Fluor 633, conjugated secondary antibodies at 1∶200 dilution (Jackson ImmunoResearch Laboratories). Phalloidin at 1∶800 dilution was used to detect Actin (Sigma-Aldrich) and DAPI at 1∶5000 dilution (Sigma-Aldrich) to stain DNA. For double staining the first antibody (followed by its specific secondary antibody) was eluted, blocked and rinsed before addition of the second antibody.
A Leica confocal microscope and software were used. Images were processed using Adobe Photoshop.
The Nec core serpin, ΔN-Nec was incubated with PPE in PBS as previously described . Porcine pancreatic elastase (PPE) was purchased from Sigma. ΔN-Nec was purified with the IMPACT-NT protein purification system .
Garland cells were dissected and transferred to PBS solution on ice. Garland cell serpin-uptake studies were performed by incubating cells in PBS solution containing 0.2 mg/ml purified ΔN-Nec solution or 0.2 mg/ml ⊗N-Nec-PPE complex. After 20 minutes incubation at room temperature, cells were fixed and stained as described above.
Whole-mount in-situ hybridisation
The Nec antisense DIG-labelled RNA probe (cDNA nucleotides 586–1450) was prepared according to the manufacturer's instructions (Roche Biochemical).
Templates for dsRNA preparation were obtained by PCR amplification between two T7 promoter sequences. Fragments for each gene were as follows: CG8909 (nucleotides 842–1537), CG33087 (12870–13136), CG12139 (1758–2346), LpR2 (3133–3748), LpR1 (2521–3033) and arrow (1572–2144). Single-stranded RNAs were synthesized and precipitated with the MEGAscript T7 transcription kit (Ambion). dsRNA was dissolved in injection buffer (0,1 mM sodium phosphate, pH 6.8; 5 mM KCL).
cDNA preparation and quantitative Real Time–PCR
Total RNA was extracted using RNAeasy Mini Kit (Quiagen) according to the manufacturer's instructions. For qRT–PCR, 1 µg of RNA was reverse-transcribed using ThermoScript RT-PCR (Invitrogen). cDNA was quantified by real-time PCR using iQ SYBERGreen Supermix (Biorad), with the iCycler (Bio-Rad) thermocycler. Probes were normalized against a ribosomal protein 49 (rp49) control. Samples were run three times and the relative levels of a given mRNA were normalized by cycling threshold analysis (ΔCT).
qRT-PCR shows low levels of nec expression during embryonic and moderate levels during larval development. Expression levels are somewhat higher in unchallenged adults than third instar larvae, but reach very similar levels in both stages 6 h post infection. Samples: embryonic (mixed stages, 0–24 h), larval instars L1, L2, and L3, pupae P1 (12–48 h), P2 (48–96 h), Adult (1 day post hatch).
(0.07 MB TIF)
nec tissue in situs in infected larvae. (A) In situ hybridisation shows cytoplasmic nec transcript (arrow) in fat-body cells of wild-type larvae. (B) Df(2R)nec− (transcript null) fat-body cells show weak background staining, but lack strong cytoplasmic staining. (C) No nec transcript is detected in wild-type larval garland cells (white arrows). Bar is 100 micrometers.
(1.44 MB TIF)
Nec antibody staining in the garland cells with necdsRNAi knockdown, 6 h post-infection. (A) Merge shows that Nec staining remains strong despite dot-Gal4 driven knockdown, monitored by UAS-GFP (in shits; UAS-NecdsRNAi; dot-Gal4 UAS-GFP larvae). (B) Nec channel. (C) GFP channel. (D) DAPI channel. Nec (red), GFP (green) and DAPI (blue).
(1.54 MB TIF)
RNAi knockdown of nec in the fat-body eliminates detectable Nec protein uptake in garland cells (white arrow). Nec (red), Actin (green) and DAPI (blue).
(1.03 MB TIF)
Time course of Nec uptake in garland cells following infection in shits1 larvae. Strongest Nec staining was detected 6–8 h post infection. Nec (red), Actin (green) and DAPI (blue). All pictures are captured with the same settings and the same laser intensity.
(3.87 MB TIF)
Effect of silencing Drosophila LDLR-family homologues on drosomycin transcript levels, 24 hr post infection. Silencing of CG8909, LRP1-like, Megalin-like and arrow do not affect Drs transcript levels significantly compared to control wild-type flies. LpR1 silencing increases Drs transcript (200%), while LpR2 silencing causes a decrease in Drs transcript (68%).
(0.10 MB TIF)
Effect of homozygous deletions of LpR genes on Drs and nec transcript levels, either 24 hr post infection or without infection. A) Levels of nec transcript in infected Df(3R)lpr1, Df(3R)lpr2 and Df(3R)lpr1/2 adults, compared to wild-type control flies. B) Levels of Drs transcript in infected Df(3R)lpr1, Df(3R)lpr2 and Df(3R)lpr1/2 adults, compared to wild-type control flies. C) Levels of Drs transcript in uninfected Df(3R)lpr1 adults, compared to infected and uninfected wild-type control flies. Deletion of the LpR1 transcript increases Drs transcript levels 1030% in compared to wild-type, in uninfected adults.
(0.82 MB TIF)
LpR2 and Nec co-localize in garland and pericardial cells. (A–D) larval, (A′–D′) adult garland cells, (A″–D″) larval and (A‴–D‴) adult pericardial cells, 6 h post infection. (A, A′, A″ and A‴) Merge shows LpR2 and Nec co-localizing (yellow) in endosomes. (B, B′, B″ and B‴) Nec channel. (C, C′, C″ and C‴) LpR2 channel. (D, D′, D″ and D‴) DAPI channel. Nec (red), LpR2 (green), and DAPI (blue).
(4.61 MB TIF)
The authors wish to thank the following for gifts of antibodies: M. Gonzalez-Gaitan (anti-Rab5), H. Bellen (anti-HRS), H. Stenmark (anti-Fab1 and anti-Lamp1), J-M Reichhart (anti-Nec and anti-Spn27A), and T. Nakamura (anti-Rab7). We also thank the Bloomington Drosophila Stock Centre for providing fly strains, D. Kimbrell for dot-Gal4, UAS-GFP, and M. Gonzalez-Gaitan for UAS-Rab5S43 and UAS-Rab7Q67L. We thank T. Dafforn and Jean-Marc Reichhart for critical discussions.
Conceived and designed the experiments: SFS DG. Performed the experiments: SFS. Analyzed the data: SFS. Contributed reagents/materials/analysis tools: JC. Wrote the paper: SFS DG.
- 1. Lemaitre B, Hoffmann J (2007) The host defense of Drosophila melanogaster. Annu Rev Immunol 25: 697–743.B. LemaitreJ. Hoffmann2007The host defense of Drosophila melanogaster.Annu Rev Immunol25697743
- 2. Levashina EA, Langley E, Green C, Gubb D, Ashburner M, et al. (1999) Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila. Science 285: 1917–1919.EA LevashinaE. LangleyC. GreenD. GubbM. Ashburner1999Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila.Science28519171919
- 3. Pelte N, Robertson AS, Zou Z, Belorgey D, Dafforn TR, et al. (2006) Immune challenge induces N-terminal cleavage of the Drosophila serpin Necrotic. Insect Biochem Mol Biol 36: 37–46.N. PelteAS RobertsonZ. ZouD. BelorgeyTR Dafforn2006Immune challenge induces N-terminal cleavage of the Drosophila serpin Necrotic.Insect Biochem Mol Biol363746
- 4. Bruce D, Perry DJ, Borg JY, Carrell RW, Wardell MR (1994) Thromboembolic disease due to thermolabile conformational changes of antithrombin Rouen-VI (187 Asn→Asp). J Clin Invest 94: 2265–2274.D. BruceDJ PerryJY BorgRW CarrellMR Wardell1994Thromboembolic disease due to thermolabile conformational changes of antithrombin Rouen-VI (187 Asn→Asp).J Clin Invest9422652274
- 5. Lomas DA, Evans DL, Finch JT, Carrell RW (1992) The mechanism of Z alpha 1-antitrypsin accumulation in the liver. Nature 357: 605–607.DA LomasDL EvansJT FinchRW Carrell1992The mechanism of Z alpha 1-antitrypsin accumulation in the liver.Nature357605607
- 6. Cicardi M, Bergamaschini L, Cugno M, Beretta A, Zingale LC, et al. (1998) Pathogenetic and clinical aspects of C1 inhibitor deficiency. Immunobiology 199: 366–376.M. CicardiL. BergamaschiniM. CugnoA. BerettaLC Zingale1998Pathogenetic and clinical aspects of C1 inhibitor deficiency.Immunobiology199366376
- 7. Providence KM, Higgins PJ (2004) PAI-1 expression is required for epithelial cell migration in two distinct phases of in vitro wound repair. J Cell Physiol 200: 297–308.KM ProvidencePJ Higgins2004PAI-1 expression is required for epithelial cell migration in two distinct phases of in vitro wound repair.J Cell Physiol200297308
- 8. Kinghorn KJ, Crowther DC, Sharp LK, Nerelius C, Davis RL, et al. (2006) Neuroserpin binds Abeta and is a neuroprotective component of amyloid plaques in Alzheimer disease. J Biol Chem 281: 29268–29277.KJ KinghornDC CrowtherLK SharpC. NereliusRL Davis2006Neuroserpin binds Abeta and is a neuroprotective component of amyloid plaques in Alzheimer disease.J Biol Chem2812926829277
- 9. Lomas DA, Belorgey D, Mallya M, Miranda E, Kinghorn KJ, et al. (2005) Molecular mousetraps and the serpinopathies. Biochem Soc Trans 33: 321–330.DA LomasD. BelorgeyM. MallyaE. MirandaKJ Kinghorn2005Molecular mousetraps and the serpinopathies.Biochem Soc Trans33321330
- 10. Green C, Levashina E, McKimmie C, Dafforn T, Reichhart JM, et al. (2000) The necrotic gene in Drosophila corresponds to one of a cluster of three serpin transcripts mapping at 43A1.2. Genetics 156: 1117–1127.C. GreenE. LevashinaC. McKimmieT. DaffornJM Reichhart2000The necrotic gene in Drosophila corresponds to one of a cluster of three serpin transcripts mapping at 43A1.2.Genetics15611171127
- 11. Carrell R, Corral J (2004) What can Drosophila tell us about serpins, thrombosis and dementia? Bioessays 26: 1–5.R. CarrellJ. Corral2004What can Drosophila tell us about serpins, thrombosis and dementia?Bioessays2615
- 12. Huntington JA, Read RJ, Carrell RW (2000) Structure of a serpin-protease complex shows inhibition by deformation. Nature 407: 923–926.JA HuntingtonRJ ReadRW Carrell2000Structure of a serpin-protease complex shows inhibition by deformation.Nature407923926
- 13. Shifman MA, Pizzo SV (1982) The in vivo metabolism of antithrombin III and antithrombin III complexes. J Biol Chem 257: 3243–3248.MA ShifmanSV Pizzo1982The in vivo metabolism of antithrombin III and antithrombin III complexes.J Biol Chem25732433248
- 14. Kounnas MZ, Church FC, Argraves WS, Strickland DK (1996) Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha 1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein. J Biol Chem 271: 6523–6529.MZ KounnasFC ChurchWS ArgravesDK Strickland1996Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha 1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein.J Biol Chem27165236529
- 15. Kasza A, Petersen HH, Heegaard CW, Oka K, Christensen A, et al. (1997) Specificity of serine proteinase/serpin complex binding to very-low-density lipoprotein receptor and alpha2-macroglobulin receptor/low-density-lipoprotein-receptor-related protein. Eur J Biochem 248: 270–281.A. KaszaHH PetersenCW HeegaardK. OkaA. Christensen1997Specificity of serine proteinase/serpin complex binding to very-low-density lipoprotein receptor and alpha2-macroglobulin receptor/low-density-lipoprotein-receptor-related protein.Eur J Biochem248270281
- 16. Strickland DK, Gonias SL, Argraves WS (2002) Diverse roles for the LDL receptor family. Trends Endocrinol Metab 13: 66–74.DK StricklandSL GoniasWS Argraves2002Diverse roles for the LDL receptor family.Trends Endocrinol Metab136674
- 17. Hussaini IM, Brown MD, Karns LR, Carpenter J, Redpath GT, et al. (1999) Epidermal growth factor differentially regulates low density lipoprotein receptor-related protein gene expression in neoplastic and fetal human astrocytes. Glia 25: 71–84.IM HussainiMD BrownLR KarnsJ. CarpenterGT Redpath1999Epidermal growth factor differentially regulates low density lipoprotein receptor-related protein gene expression in neoplastic and fetal human astrocytes.Glia257184
- 18. Gent J, Braakman I (2004) Low-density lipoprotein receptor structure and folding. Cell Mol Life Sci 61: 2461–2470.J. GentI. Braakman2004Low-density lipoprotein receptor structure and folding.Cell Mol Life Sci6124612470
- 19. Guimond S, Turnbull JE (2004) Proteoglycans make the grade-ient. Mol Cell 16: 159–160.S. GuimondJE Turnbull2004Proteoglycans make the grade-ient.Mol Cell16159160
- 20. Beglova N, Jeon H, Fisher C, Blacklow SC (2004) Structural features of the low-density lipoprotein receptor facilitating ligand binding and release. Biochem Soc Trans 32: 721–723.N. BeglovaH. JeonC. FisherSC Blacklow2004Structural features of the low-density lipoprotein receptor facilitating ligand binding and release.Biochem Soc Trans32721723
- 21. Praefcke GJ, McMahon HT (2004) The dynamin superfamily: universal membrane tubulation and fission molecules? Nat Rev Mol Cell Biol 5: 133–147.GJ PraefckeHT McMahon2004The dynamin superfamily: universal membrane tubulation and fission molecules?Nat Rev Mol Cell Biol5133147
- 22. Zerial M, McBride H (2001) Rab proteins as membrane organizers. Nat Rev Mol Cell Biol 2: 107–117.M. ZerialH. McBride2001Rab proteins as membrane organizers.Nat Rev Mol Cell Biol2107117
- 23. Lakadamyali M, Rust MJ, Zhuang X (2006) Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes. Cell 124: 997–1009.M. LakadamyaliMJ RustX. Zhuang2006Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes.Cell1249971009
- 24. Kramer H, Phistry M (1999) Genetic analysis of hook, a gene required for endocytic trafficking in drosophila. Genetics 151: 675–684.H. KramerM. Phistry1999Genetic analysis of hook, a gene required for endocytic trafficking in drosophila.Genetics151675684
- 25. Lakkaraju A, Rodriguez-Boulan E (2008) Itinerant exosomes: emerging roles in cell and tissue polarity. Trends Cell Biol 18: 199–209.A. LakkarajuE. Rodriguez-Boulan2008Itinerant exosomes: emerging roles in cell and tissue polarity.Trends Cell Biol18199209
- 26. Lloyd TE, Atkinson R, Wu MN, Zhou Y, Pennetta G, et al. (2002) Hrs regulates endosome membrane invagination and tyrosine kinase receptor signaling in Drosophila. Cell 108: 261–269.TE LloydR. AtkinsonMN WuY. ZhouG. Pennetta2002Hrs regulates endosome membrane invagination and tyrosine kinase receptor signaling in Drosophila.Cell108261269
- 27. Rusten TE, Stenmark H (2006) Analyzing phosphoinositides and their interacting proteins. Nat Methods 3: 251–258.TE RustenH. Stenmark2006Analyzing phosphoinositides and their interacting proteins.Nat Methods3251258
- 28. Rusten TE, Vaccari T, Lindmo K, Rodahl LM, Nezis IP, et al. (2007) ESCRTs and Fab1 regulate distinct steps of autophagy. Curr Biol 17: 1817–1825.TE RustenT. VaccariK. LindmoLM RodahlIP Nezis2007ESCRTs and Fab1 regulate distinct steps of autophagy.Curr Biol1718171825
- 29. Simonsen A, Cumming RC, Lindmo K, Galaviz V, Cheng S, et al. (2007) Genetic modifiers of the Drosophila blue cheese gene link defects in lysosomal transport with decreased life span and altered ubiquitinated-protein profiles. Genetics 176: 1283–1297.A. SimonsenRC CummingK. LindmoV. GalavizS. Cheng2007Genetic modifiers of the Drosophila blue cheese gene link defects in lysosomal transport with decreased life span and altered ubiquitinated-protein profiles.Genetics17612831297
- 30. Kosaka T, Ikeda K (1983) Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature-sensitive mutant of Drosophila melanogaster, shibirets1. J Cell Biol 97: 499–507.T. KosakaK. Ikeda1983Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature-sensitive mutant of Drosophila melanogaster, shibirets1.J Cell Biol97499507
- 31. Narita K, Tsuruhara T, Koenig JH, Ikeda K (1989) Membrane pinch-off and reinsertion observed in living cells of Drosophila. J Cell Physiol 141: 383–391.K. NaritaT. TsuruharaJH KoenigK. Ikeda1989Membrane pinch-off and reinsertion observed in living cells of Drosophila.J Cell Physiol141383391
- 32. Chang HC, Newmyer SL, Hull MJ, Ebersold M, Schmid SL, et al. (2002) Hsc70 is required for endocytosis and clathrin function in Drosophila. J Cell Biol 159: 477–487.HC ChangSL NewmyerMJ HullM. EbersoldSL Schmid2002Hsc70 is required for endocytosis and clathrin function in Drosophila.J Cell Biol159477487
- 33. Chang HC, Hull M, Mellman I (2004) The J-domain protein Rme-8 interacts with Hsc70 to control clathrin-dependent endocytosis in Drosophila. J Cell Biol 164: 1055–1064.HC ChangM. HullI. Mellman2004The J-domain protein Rme-8 interacts with Hsc70 to control clathrin-dependent endocytosis in Drosophila.J Cell Biol16410551064
- 34. Aggarwal SK, King RC (1967) The ultrastructure of the wreath cells of Drosophila melanogaster larvae. Protoplasma 63: 343–352.SK AggarwalRC King1967The ultrastructure of the wreath cells of Drosophila melanogaster larvae.Protoplasma63343352
- 35. Das D, Ashoka D, Aradhya R, Inamdar M (2008) Gene expression analysis in post-embryonic pericardial cells of Drosophila. Gene Expr Patterns 8: 199–205.D. DasD. AshokaR. AradhyaM. Inamdar2008Gene expression analysis in post-embryonic pericardial cells of Drosophila.Gene Expr Patterns8199205
- 36. Kimbrell DA, Hice C, Bolduc C, Kleinhesselink K, Beckingham K (2002) The Dorothy enhancer has Tinman binding sites and drives hopscotch-induced tumor formation. Genesis 34: 23–28.DA KimbrellC. HiceC. BolducK. KleinhesselinkK. Beckingham2002The Dorothy enhancer has Tinman binding sites and drives hopscotch-induced tumor formation.Genesis342328
- 37. Sanger JW, McCann FV (1968) Ultrastructure of the myocardium of the moth, Hyalophora cecropia. J Insect Physiol 14: 1105–1111.JW SangerFV McCann1968Ultrastructure of the myocardium of the moth, Hyalophora cecropia.J Insect Physiol1411051111
- 38. Thomsen M (1951) Weismann's ring and related organs in larvae of diptera. . Danske Selsk Biol Skr 6: 1–32.M. Thomsen1951Weismann's ring and related organs in larvae of diptera. .Danske Selsk Biol Skr6132
- 39. Locke R (1998) pericardial cells or athrocytes. Wiley-Liss. pp. 687–709.R. Locke1998pericardial cells or athrocytesWiley-Liss687709
- 40. Weavers H, Prieto-Sanchez S, Grawe F, Garcia-Lopez A, Artero R, et al. (2009) The insect nephrocyte is a podocyte-like cell with a filtration slit diaphragm. Nature 457: 322–6.H. WeaversS. Prieto-SanchezF. GraweA. Garcia-LopezR. Artero2009The insect nephrocyte is a podocyte-like cell with a filtration slit diaphragm.Nature4573226
- 41. Mills RP, King RC (1965) The pericardial cells of Drosophila melanogaster. Q J Microsc Sci 106: 261–268.RP MillsRC King1965The pericardial cells of Drosophila melanogaster.Q J Microsc Sci106261268
- 42. Das D, Aradhya R, Ashoka D, Inamdar M (2008) Macromolecular uptake in Drosophila pericardial cells requires rudhira function. Exp Cell Res 314: 1804–1810.D. DasR. AradhyaD. AshokaM. Inamdar2008Macromolecular uptake in Drosophila pericardial cells requires rudhira function.Exp Cell Res31418041810
- 43. Wigglesworth VB (1970) The pericardial cells of insects: analogue of the reticuloendothelial system. J Reticuloendothel Soc 7: 208–216.VB Wigglesworth1970The pericardial cells of insects: analogue of the reticuloendothelial system.J Reticuloendothel Soc7208216
- 44. Amrite AC, Edelhauser HF, Singh SR, Kompella UB (2008) Effect of circulation on the disposition and ocular tissue distribution of 20 nm nanoparticles after periocular administration. Mol Vis 14: 150–160.AC AmriteHF EdelhauserSR SinghUB Kompella2008Effect of circulation on the disposition and ocular tissue distribution of 20 nm nanoparticles after periocular administration.Mol Vis14150160
- 45. Martin-Armas M, Zykova S, Smedsrod B (2008) Effects of CpG-oligonucleotides, poly I∶C and LPS on Atlantic cod scavenger endothelial cells (SEC). Dev Comp Immunol 32: 100–107.M. Martin-ArmasS. ZykovaB. Smedsrod2008Effects of CpG-oligonucleotides, poly I∶C and LPS on Atlantic cod scavenger endothelial cells (SEC).Dev Comp Immunol32100107
- 46. Wagner HN Jr, Iio M (1964) Studies of the Reticuloendothelial System (Res). 3. Blockade of the Res in Man. J Clin Invest 43: 1525–1532.HN Wagner JrM. Iio1964Studies of the Reticuloendothelial System (Res). 3. Blockade of the Res in Man.J Clin Invest4315251532
- 47. De Gregorio E, Spellman PT, Rubin GM, Lemaitre B (2001) Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays. Proc Natl Acad Sci U S A 98: 12590–12595.E. De GregorioPT SpellmanGM RubinB. Lemaitre2001Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays.Proc Natl Acad Sci U S A981259012595
- 48. Sriram V, Krishnan KS, Mayor S (2003) deep-orange and carnation define distinct stages in late endosomal biogenesis in Drosophila melanogaster. J Cell Biol 161: 593–607.V. SriramKS KrishnanS. Mayor2003deep-orange and carnation define distinct stages in late endosomal biogenesis in Drosophila melanogaster.J Cell Biol161593607
- 49. Lindmo K, Simonsen A, Brech A, Finley K, Rusten TE, et al. (2006) A dual function for Deep orange in programmed autophagy in the Drosophila melanogaster fat body. Exp Cell Res 312: 2018–2027.K. LindmoA. SimonsenA. BrechK. FinleyTE Rusten2006A dual function for Deep orange in programmed autophagy in the Drosophila melanogaster fat body.Exp Cell Res31220182027
- 50. Kramer H (2002) Sorting out signals in fly endosomes. Traffic 3: 87–91.H. Kramer2002Sorting out signals in fly endosomes.Traffic38791
- 51. Goto A, Blandin S, Royet J, Reichhart JM, Levashina EA (2003) Silencing of Toll pathway components by direct injection of double-stranded RNA into Drosophila adult flies. Nucleic Acids Res 31: 6619–6623.A. GotoS. BlandinJ. RoyetJM ReichhartEA Levashina2003Silencing of Toll pathway components by direct injection of double-stranded RNA into Drosophila adult flies.Nucleic Acids Res3166196623
- 52. Robertson AS, Belorgey D, Lilley KS, Lomas DA, Gubb D, et al. (2003) Characterization of the necrotic protein that regulates the Toll-mediated immune response in Drosophila. J Biol Chem 278: 6175–6180.AS RobertsonD. BelorgeyKS LilleyDA LomasD. Gubb2003Characterization of the necrotic protein that regulates the Toll-mediated immune response in Drosophila.J Biol Chem27861756180
- 53. Reichhart JM (2005) Tip of another iceberg: Drosophila serpins. Trends Cell Biol 15: 659–665.JM Reichhart2005Tip of another iceberg: Drosophila serpins.Trends Cell Biol15659665
- 54. Ligoxygakis P, Roth S, Reichhart JM (2003) A serpin regulates dorsal-ventral axis formation in the Drosophila embryo. Curr Biol 13: 2097–2102.P. LigoxygakisS. RothJM Reichhart2003A serpin regulates dorsal-ventral axis formation in the Drosophila embryo.Curr Biol1320972102
- 55. Ligoxygakis P, Pelte N, Ji C, Leclerc V, Duvic B, et al. (2002) A serpin mutant links Toll activation to melanization in the host defence of Drosophila. Embo J 21: 6330–6337.P. LigoxygakisN. PelteC. JiV. LeclercB. Duvic2002A serpin mutant links Toll activation to melanization in the host defence of Drosophila.Embo J2163306337
- 56. Scherfer C, Tang H, Kambris Z, Lhocine N, Hashimoto C, et al. (2008) Drosophila Serpin-28D regulates hemolymph phenoloxidase activity and adult pigmentation. Dev Biol 323: 189–196.C. ScherferH. TangZ. KambrisN. LhocineC. Hashimoto2008Drosophila Serpin-28D regulates hemolymph phenoloxidase activity and adult pigmentation.Dev Biol323189196
- 57. Tang H, Kambris Z, Lemaitre B, Hashimoto C (2008) A serpin that regulates immune melanization in the respiratory system of Drosophila. Dev Cell 15: 617–626.H. TangZ. KambrisB. LemaitreC. Hashimoto2008A serpin that regulates immune melanization in the respiratory system of Drosophila.Dev Cell15617626
- 58. Oley M, Letzel MC, Ragg H (2004) Inhibition of furin by serpin Spn4A from Drosophila melanogaster. FEBS Lett 577: 165–169.M. OleyMC LetzelH. Ragg2004Inhibition of furin by serpin Spn4A from Drosophila melanogaster.FEBS Lett577165169
- 59. Das D, Aradhya R, Ashoka D, Inamdar M (2008) Post-embryonic pericardial cells of Drosophila are required for overcoming toxic stress but not for cardiac function or adult development. Cell Tissue Res 331: 565–570.D. DasR. AradhyaD. AshokaM. Inamdar2008Post-embryonic pericardial cells of Drosophila are required for overcoming toxic stress but not for cardiac function or adult development.Cell Tissue Res331565570
- 60. Ferrandon D, Jung AC, Criqui M, Lemaitre B, Uttenweiler-Joseph S, et al. (1998) A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway. Embo J 17: 1217–1227.D. FerrandonAC JungM. CriquiB. LemaitreS. Uttenweiler-Joseph1998A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway.Embo J1712171227
- 61. Hollande C (1921) La cellule pericardiale des insectes. Arch Anat Microsc 18: 85–307.C. Hollande1921La cellule pericardiale des insectes.Arch Anat Microsc1885307
- 62. Perlmutter DH, Glover GI, Rivetna M, Schasteen CS, Fallon RJ (1990) Identification of a serpin-enzyme complex receptor on human hepatoma cells and human monocytes. Proc Natl Acad Sci U S A 87: 3753–3757.DH PerlmutterGI GloverM. RivetnaCS SchasteenRJ Fallon1990Identification of a serpin-enzyme complex receptor on human hepatoma cells and human monocytes.Proc Natl Acad Sci U S A8737533757
- 63. Green C, Brown G, Dafforn TR, Reichhart JM, Morley T, et al. (2003) Drosophila necrotic mutations mirror disease-associated variants of human serpins. Development 130: 1473–1478.C. GreenG. BrownTR DaffornJM ReichhartT. Morley2003Drosophila necrotic mutations mirror disease-associated variants of human serpins.Development13014731478
- 64. Gubb D, Green C, Huen D, Coulson D, Johnson G, et al. (1999) The balance between isoforms of the prickle LIM domain protein is critical for planar polarity in Drosophila imaginal discs. Genes Dev 13: 2315–2327.D. GubbC. GreenD. HuenD. CoulsonG. Johnson1999The balance between isoforms of the prickle LIM domain protein is critical for planar polarity in Drosophila imaginal discs.Genes Dev1323152327
- 65. Entchev EV, Schwabedissen A, Gonzalez-Gaitan M (2000) Gradient formation of the TGF-beta homolog Dpp. Cell 103: 981–991.EV EntchevA. SchwabedissenM. Gonzalez-Gaitan2000Gradient formation of the TGF-beta homolog Dpp.Cell103981991
- 66. Wolff T (2000) Histological Techniques for Drosophila Eye Part1 and 2. In: Sullivan W. AM, editor. Drosophila Protocols. New York: Cold Spring Habor Laboratory Press. T. Wolff2000Histological Techniques for Drosophila Eye Part1 and 2.AM Sullivan W.Drosophila ProtocolsNew YorkCold Spring Habor Laboratory Press728
- 67. Tanaka T, Nakamura A (2008) The endocytic pathway acts downstream of Oskar in Drosophila germ plasm assembly. Development 135: 1107–1117.T. TanakaA. Nakamura2008The endocytic pathway acts downstream of Oskar in Drosophila germ plasm assembly.Development13511071117
- 68. Wucherpfennig T, Wilsch-Brauninger M, Gonzalez-Gaitan M (2003) Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release. J Cell Biol 161: 609–624.T. WucherpfennigM. Wilsch-BrauningerM. Gonzalez-Gaitan2003Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release.J Cell Biol161609624
- 69. Ohno H, Stewart J, Fournier MC, Bosshart H, Rhee I, et al. (1995) Interaction of tyrosine-based sorting signals with clathrin-associated proteins. Science 269: 1872–1875.H. OhnoJ. StewartMC FournierH. BosshartI. Rhee1995Interaction of tyrosine-based sorting signals with clathrin-associated proteins.Science26918721875
- 70. Steiner P, Sarria JC, Glauser L, Magnin S, Catsicas S, et al. (2002) Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD. J Cell Biol 157: 1197–1209.P. SteinerJC SarriaL. GlauserS. MagninS. Catsicas2002Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD.J Cell Biol15711971209