To faithfully encode mechanosensory information, auditory/vestibular hair cells utilize graded synaptic vesicle (SV) release at specialized ribbon synapses. The molecular basis of SV release and consequent recycling of membrane in hair cells has not been fully explored. Here, we report that comet, a gene identified in an ENU mutagenesis screen for zebrafish larvae with vestibular defects, encodes the lipid phosphatase Synaptojanin 1 (Synj1). Examination of mutant synj1 hair cells revealed basal blebbing near ribbons that was dependent on Cav1.3 calcium channel activity but not mechanotransduction. Synaptojanin has been previously implicated in SV recycling; therefore, we tested synaptic transmission at hair-cell synapses. Recordings of post-synaptic activity in synj1 mutants showed relatively normal spike rates when hair cells were mechanically stimulated for a short period of time at 20 Hz. In contrast, a sharp decline in the rate of firing occurred during prolonged stimulation at 20 Hz or stimulation at a higher frequency of 60 Hz. The decline in spike rate suggested that fewer vesicles were available for release. Consistent with this result, we observed that stimulated mutant hair cells had decreased numbers of tethered and reserve-pool vesicles in comparison to wild-type hair cells. Furthermore, stimulation at 60 Hz impaired phase locking of the postsynaptic activity to the mechanical stimulus. Following prolonged stimulation at 60 Hz, we also found that mutant synj1 hair cells displayed a striking delay in the recovery of spontaneous activity. Collectively, the data suggest that Synj1 is critical for retrieval of membrane in order to maintain the quantity, timing of fusion, and spontaneous release properties of SVs at hair-cell ribbon synapses.
Ribbon synapses are found in the ear and eye and facilitate the transmission of sensory information to the brain. In hair cells of the ear, the molecules required for ribbon function have not been fully explored. Zebrafish are ideal for investigating molecular components of these specialized synapses because of the ability to study ribbon function using genetic, cellular, and physiological methods. Here, we explore the role of the lipid phosphatase Synaptojanin at the hair cell synapse. Synaptojanin has been previously implicated in synaptic vesicle recycling in conventional synapses, and we also find that the number of synaptic vesicles are reduced in mutant synaptojanin hair cells. Mutant synaptojanin larvae have obvious equilibrium defects, and our electrophysiological recordings revealed that synaptic transmission from hair cells to neurons projecting to the brain is impaired in terms of both rate and accuracy. When stimulated at high frequency or for prolonged periods, mutant synaptojanin hair cells release vesicles out of phase with mechanical stimuli, thus compromising the transfer of sensory information to the brain.
Citation: Trapani JG, Obholzer N, Mo W, Brockerhoff SE, Nicolson T (2009) synaptojanin1 Is Required for Temporal Fidelity of Synaptic Transmission in Hair Cells. PLoS Genet 5(5): e1000480. https://doi.org/10.1371/journal.pgen.1000480
Editor: Tobias Moser, University of Göttingen, Germany
Received: February 24, 2009; Accepted: April 10, 2009; Published: May 8, 2009
Copyright: © 2009 Trapani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was supported in part by NIH R01 DC006880, NIH PA-01-011 funds and NIH RO1 EY015165. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Vertebrate hair cells generate graded receptor potentials in response to mechanical stimuli, resulting in neurotransmitter release with high temporal accuracy and fidelity. Precise timing of release supports the phase locking of post-synaptic activity to stimuli in the kilohertz range . These high rates of transmitter release require the specialized ribbon synapse –. For example, in hair cells of the mouse inner ear, ribbon synapses support remarkable rates of exocytosis approaching 1000 SV/sec ,. Such high rates are probably achieved by ribbon-mediated rapid exocytosis of a large pool of readily releasable vesicles , –.
The role of the ribbon in orchestrating timed SV release is not fully characterized. Perhaps specialized, ribbon-associated proteins are required for accurate timing of release. Additionally, the availability of vesicles at hair-cell ribbons may be necessary and sufficient for both release rate and timing. A recent modeling study of ribbon transmission depicts how increased numbers of docked, releasable vesicles reduces both the latency of release and the associated temporal jitter at ribbon synapses . To maintain a steady supply of SVs at the ribbon, hair cells maintain a large reserve pool of vesicles. Thus, thousands of SVs can be released in response to strong stimulation with membrane turnover approaching a quantity of half the cell surface area within seconds ,. It has been shown that disruption in vesicle recycling results in a reduced transmission at neuromuscular junctions . An effect on temporal precision of release in hair-cell synapses has not been investigated in vivo.
A key player in vesicle recycling is synaptojanin1 (Synj1; reviewed by ). Synj1 is a multidomain lipid-phosphatase that contains a sac1-like phosphatase domain, a 5-phosphatase domain, and a proline-rich domain (PRD). Synj1 regulates phosphoinositide turnover, especially that of phosphatidylinositol (4,5) bisphosphate (PI(4,5)P2), resulting in the shedding of the clathrin coat on internalized vesicles ,. In zebrafish, synj1-deficient nrc (no optokinetic response ‘c’) mutants have visual defects and floating synaptic ribbons in photoreceptors, but not bipolar cells ,. Van Epps et al. noted an affect on balance, but did not examine the expression and role of synj1 in hair cells .
We report here that the comet gene, which was isolated during a large-scale mutagenesis screen, encodes synj1. However, mutant zebrafish larvae display balance defects and a corresponding impaired vestibulo-ocular reflex. Given the previously described role of synj1 in SV recycling, we examined synaptic transmission and vesicle pools in mutant hair cells. We found a decrease in spike rate along with a decrease in number of ribbon-associated SVs. Our recordings also revealed an impairment of phase locking in synj1 mutants and a striking delay in the return of spontaneous release of SVs following periods of prolonged stimulation in mutants. Taken together, these results suggest that active maintenance of vesicle pools is required for robust and accurate timing of release of SVs in hair cells.
Genetic Lesions and Expression of synj1 in Hair Cells
Three comet mutants were isolated in a large-scale chemical mutagenesis screen for larvae with balance defects. To characterize the mutant comet phenotype at the molecular level, we mapped the comet gene and noted that the recessive lesions mapped closely to a previously identified locus of the nrc gene . The nrc mutant has a nonsense mutation in synj1, leading to visual defects. We confirmed the identity of comet as synj1 by complementation analysis with nrc heterozygous fish. Sequencing revealed that two alleles, synj1Q296X and synj1W943X, contain nonsense mutations that lead to truncations of the protein product within the first and after the second phosphatase domain, respectively (Figure 1A). The genomic lesion of the third allele, synj1c.188+2T>A, inactivates the donor splice site of exon 2 that contains the translation start site (ATG), thereby leading to the deletion of exon 2 and presumably no protein product (Figure 1A). The synj1Q296X allele causes a truncation within the SAC1 domain similar to the original nrc allele (R499X), which results in undetectable levels of Synj1 protein in larval brain extracts . Because differences in behavior, cellular or physiological phenotypes could not be detected among larvae carrying the three alleles, we conducted our experiments with synj1Q296X mutants.
A) Splice diagram of the zebrafish synj1 gene indicating alternate splicing of exons 13, 14 and 15, which can lead to the inclusion or exclusion of other indicated sequences (brackets). The diagram was assembled from collective synj1 sequencing data. Three new mutations of synj1 include deletion of the translation start site (donor splice site inactivation), and nonsense mutations, resulting in truncations at amino acid 296, or amino acid 943. A color-coded asterisk above the splice diagram indicates the position of the respective mutation in the transcript, and the expected protein product is depicted after the allele identifier, followed by the mutated nucleotide sequence. B,C) Expression of synj1 in inner ear and neuromast hair cells. B) synj1 expression pattern in the developing larva as shown by in situ hybridization. At 120 hpf, synj1 is expressed at high levels in the brain, eye and inner ear (arrow; upper panel). Hair-cell specific expression (asterisk) was observed in transverse thick sections of the ear at 100× magnification; both anti-sense synj1 and sense control label are shown (left and right lower panels, respectively). C) RT-PCR for synj1 from isolated neuromast hair-cells amplified the short (synj1-145) isoform. The panel depicts, from left to right, four control reactions (no template) and four hair-cell mRNA template reactions. gapdh mRNA was used as a positive control (band at 151 bp). Specific primer pairs were used to selectively amplify the long and short isoforms sj170 (101 bp expected band size) and sj145 (200 bp), respectively. The sj1 indicates total synj1 mRNA (140 bp).
Next we investigated synj1 expression in the larval auditory/vestibular system. In 96–120 hours post fertilization (hpf) zebrafish larvae, we detected synj1 mRNA expression in the central nervous system: foremost in the brain, retina, and in the ear (Figure 1B, upper panel). Sections of the developing ear showed expression of synj1 mRNA in hair cells (Figure 1B, lower left panel). In addition to visualizing synj1 expression by in situ hybridization, we injected a plasmid with a 6.5 kb fragment of the synj1 promoter driving EGFP and observed transient EGFP expression in the nervous system and in hair cells of both the ear and lateral line (data not shown). Our results confirm a previous report of expression of synj1 in zebrafish hair cells using microarray analysis . Using RT-PCR on tissue isolated from neuromasts, we detected the synj1-145 but not the synj1-170 isoform, suggesting that the short splice variant is specific to hair cells (Figure 1C). As positive controls, PCR reactions using random decamer mRNA from whole larvae showed robust amplification with each primer set (data not shown). In addition to in situ hybridization and RT-PCR of isolated neuromasts, we attempted to label Synj1 with antibodies, but were not successful with either published  or newly generated antibodies.
The characteristic tilting posture displayed by synj1 mutant larvae is indicative of vestibular dysfunction. When mutant larvae were continuously swirled in a Petri dish of water they adopted more severely affected postures, including floating upside down. To determine the extent to which vestibular function was affected in synj1Q296X mutants, we tested the vestibulo-ocular reflex (VOR) in 120 hpf larvae. The VOR is an involuntary compensatory eye movement in response to stimulation of the vestibular system. The VOR in response to rotation of the head was significantly reduced in synj1Q296X mutants (Figure 2A). We observed a 48% reduction of mean relative eye movement (quantified by the total power at 0.2 Hz) in mutants compared to wild-type siblings (Figure 2B). Although the response is clearly present, these data suggest that transmission of vestibular information in synj1 mutants is impaired.
Shown in (A) are average relative eye movements of both eyes in wild type (WT; black circles) and synj1Q296X mutant (grey circles) larvae in response to 0.2 Hz stimulation (n>9). B) Plot of power at 0.2 Hz for both eyes for each individual WT (black circles) and mutant (grey circles) larva shown in (A). All values were normalized to the largest WT value. The mean power is represented by the black horizontal lines.
Basal Blebbing in Mutant synj1 Hair Cells Is Dependent on Cav1.3 Channels
Unlike mutant synj1 photoreceptor ribbons, the ribbons in synj1Q296X hair cells are localized to the plasma membrane (Figure S1 and Figure 3H). However, we detected the presence of large basal membrane protrusions or blebs in approximately a third of mutant hair cells (34.5±0.9%, n = 388 cells; Figure 3). Large blebs emanating from mutant hair cells could be visualized in live, intact fish using the vital dye, FM1-43 (Figure 3B, arrow). Blebbing was also observed in inner ear or neuromast mutant hair cells transiently expressing soluble GFP (plasmid myo6b∶GFP; data not shown), and in mutant hair cells stably expressing a membrane-targeted form of GFP (Tg(brn3c∶mGFP); Figure 3F). When counterstained with anti-Ribeye b antibody, we observed that the extrusions of membrane occurred mainly near ribbons (Figure 3H). In addition, we examined the synaptic vesicle marker Vglut3 using immunofluorescence and saw that Vglut3 was present but not noticeably enriched in blebbing membranes (Figure S2). We also transiently expressed a GFP-tagged pleckstrin homology domain of phospholipase Cδ1 protein that binds to PI(4,5)P2  and did not note any obvious elevation in PI(4,5)P2 in mutant hair cells (data not shown).
FM 1–43 labeling of wild-type (A) and synj1Q296X (B) neuromast hair cells. Note the bleb or membrane protrusion at the base of a mutant cell (arrow). Single optical sections of membrane-targeted EGFP (Tg(brn3c∶mgfp)) expressed in wild-type (C) and mutant (F) hair cells. D, G) Ribeye-b immunolabel (red staining) of the same neuromasts, indicating ribbons. E, H) Overlays of membrane-targeted EGFP and Ribeye-b label. Arrows in panel H indicate blebs near the ribbon in mutant hair cells. I) Basal blebbing was Ca2+-dependent. Blebbing occurred minimally in neuromast hair-cells of wild-type larvae (black bar). In contrast, blebbing was significantly increased in synj1Q296X mutant hair cells (grey bar). Blebbing was minimal in cav1.3R1250X mutant hair cells (blue bar), as well as cav1.3R1250X/synj1Q296X double mutant hair cells (blue stripe bar). Blebbing still occurred in synj1Q296X mutants that were transduction-deficient by morpholino-mediated cadherin23 knockdown (grey stripe bar). Numbers in parentheses above bars indicate number of hair cells examined. Scale bars, 3 µm.
To determine if the basal blebbing was dependent on synaptic transmission, we generated double mutant larvae that were homozygous for synj1Q296X and cav1.3R1250X. Cav1.3 L-type calcium channels mediate influx of calcium near hair-cell ribbons, causing the fusion of SVs ,. In cav1.3aR1250X single mutants, the amount of blebbing was comparable to wild-type levels (Figure 3I; cav1.3R1250X: 5.2±0.2%, n = 358 cells, 15 larvae; wild-type: 7.3±0.2%, n = 200 cells, 8 larvae). In contrast to synj1Q296X single mutants, we observed low levels of blebbing in cav1.3R1250X/synj1Q296X double mutants (Figure 3I; 6.0±0.3%, n = 150 cells, 6 larvae). Thus, the presence of Cav1.3a calcium channels was required for the blebbing phenotype observed in mutant synj1 hair cells. In contrast to cav1.3R1250X/synj1Q296X double mutants, blebbing occurred independently of the presence of Cadherin 23, which is required for mechanotransduction (29.6±2.4%, n = 81 cells, 4 larvae; ,; Figure 3I). These results suggested that an imbalance of exo- and endocytosis led to blebbing in synj1 mutant hair cells.
Mutant synj1 Hair Cells Produce Fewer Postsynaptic Spikes during High-Frequency Stimulation
We investigated the impact of synj1 mutations on hair-cell transmission by stimulating neuromast hair cells and recording the evoked postsynaptic spikes from posterior lateral line ganglion (PLLg) cells . Initially, we quantified the spontaneous activity of neuromast hair cells by recording action currents in the absence of water-jet stimulation. Wild-type afferent neurons displayed robust spontaneous activity (6.5±1.0 spikes/sec, n = 7). There was no significant difference in spontaneous activity in synj1Q296X neurons (7.0±0.9 spikes/sec, n = 7).
To test whether mutant synapses displayed altered behavior following direct hair-cell activation, we mechanically stimulated neuromast hair cells at 20 Hz. We chose this rate or higher (see below) because lateral line hair cells are sensitive to frequencies of 1–150 Hz . Stimulation at 20 Hz resulted in no significant difference in spike rate in the first 60 seconds of stimulation (mutant: 11.4±2.4 spikes/sec, n = 6; wild-type: 11.9±3.4 spikes/sec, n = 4; Figure 4A,B). Based on the previously described role of synj1 in neurons, we hypothesized that sustained stimulation would fatigue the hair cell synapse. Indeed, stimulation of synj1Q296X hair cells at 20 Hz for 15 minutes resulted in a significant decrease in the number of spikes in synj1Q296X larvae compared to wild-type larvae (mutant: 6.6±1.5 spikes/sec, n = 6; wild-type: 11.7±3.0 spikes/sec, n = 4; Figure 4B)
A) Spikes recorded from a wild-type (black traces) and synj1Q296X (grey traces) larva in response to 20 Hz stimulation. Shown is an overlay of 60 consecutive sweeps from an individual larva. B\) Box plot summarizing the spike rate (spikes per second) for 60 seconds of 20 Hz stimulation (black bars, wild-type; grey bars, synj1Q296X; includes larvae in A. C) Spikes recorded from a wild-type (black traces) and synj1Q296X (grey traces) larva in response to 60 Hz hair-cell stimulation. Shown are 60 consecutive sweeps overlaid from a single larva. D) Box plot summarizing the reduction in spike rate of synj1Q296X (grey bars) during 60 seconds of 60 Hz stimulation compared to wild-type neurons (black bars). Following 15 minutes of sustained stimulation the synj1Q296X mutant spike rate was reduced compared to both the initial rate and the wild-type rate.
To determine whether the reduction in activity seen in synj1Q296X afferent neurons was exacerbated at higher frequency, we increased the stimulation frequency to 60 Hz. Within the first minute of 60 Hz stimulation, mutant spike rate was largely reduced compared to wild-type (mutant: 10.3±2.3 spikes/sec, n = 8; wild-type: 19.4±3.2 spikes/sec, n = 7; Figure 4C,D). Next, we attempted to fatigue the synapse with 15 minutes of sustained 60 Hz stimulation. Following 15 minutes of continuous stimulation, the mutant spike rate (5.1±1.4 spikes/sec, n = 8) was significantly reduced compared to both the wild-type rate (15.7±1.7 spikes/sec, n = 7) and its own initial release rate (Figure 4D). To rule out the possibility that the sustained stimulation affected mechanotransduction, we obtained microphonic potentials before and after 15 minutes of 60-Hz stimulation. We saw no differences between wild-type and mutants in the recordings pre-and post-stimulation (Figure S3).
SV Recycling Is Perturbed in Mutant synj1 Hair Cells
Given the previously described role for Synj1 in vesicle recycling and the decline of spiking in synj1 mutants, we examined the various membrane components in stimulated wild-type and mutant hair cells using transmission electron microscopy. For these experiments, we stimulated whole larvae for 15 minutes at 60 Hz before fixation (see Methods). To quantify changes, we used single thin sections that featured the center of ribbon and counted structures within a 450 nm radius of the ribbon in neuromast hair cells (Figure 5). In mutant hair cells, we observed an increase in the number of large coated vesicles approximately 70 nm in diameter, which is larger than a typical SV with a diameter of 40 nm (synj1Q296X :1.6±0.2 large vesicles, n = 20 ribbons; wild-type: 0.7±0.2 large vesicles, n = 14 ribbons; Figure 5C). Large coated vesicles were also reported by Lenzi et al. upon maximal stimulation of frog hair cells (2002). Small clathrin-coated vesicles (CCVs; ≥50 nm) with a diameter similar to SVs were infrequently seen near the ribbon.
A) TEM micrographs of neuromast ribbon synapses (upper panel: wild-type ribbon; lower panel: synj1Q296X mutant ribbon). B) Same micrographs as in (A) with white circles denoting tethered vesicles. Blue and orange circles highlight the reserve vesicles in the wild type and mutant synapses, respectively. Note the reduction of reserve pool vesicles in the mutant synapse. Arrow heads indicate examples of large coated vesicles. Scale bar, 200 nm. Number of large coated vesicles (C), tethered vesicles per 100 nm of ribbon perimeter (D), and reserve pool vesicles (E) within 450 nm of ribbon centers in neuromast hair cells (black bars: wild type; grey bars: synj1Q296X).
Previous synj1 mutant studies in the worm and fly neuromuscular junction have suggested that the rundown of synaptic events in these mutants is the result of SV depletion ,. We counted the SV pools surrounding hair-cell ribbons on single sections as above. We define the first shell of SVs that decorate the ribbons as ribbon-tethered. With respect to this releasable pool of SVs, we observed a reduction of approximately 30% of ribbon-tethered SVs in mutant neuromast hair cells (mutant: 1.6±0.1/100 nm of ribbon perimeter, n = 30 ribbons; wild-type: 2.2±0.1/100 nm of ribbon perimeter, n = 18 ribbons; Figure 5D). We also observed far fewer reserve pool or non-tethered vesicles within 450 nm of ribbons in mutant neuromast hair cells (mutant: 14.5±1.6, n = 13 ribbons; wild-type: 29.3±2.5, n = 11 ribbons; Figure 5E).
With the exception of a two-fold increase of large coated vesicles, we noted only minor differences in membrane compartments of inner ear hair cells (data not shown). The relatively weak effect on inner ear hair cells is likely due to the 60 Hz stimulus being suboptimal for the inner ear maculae. In zebrafish larvae, an acoustic startle reflex can be elicited with frequencies ranging from 100–1200 Hz or potentially higher, with a higher sensitivity at ≥300 Hz . The difference in sensitivity thus predicts the differential effect of the 60 Hz stimulus on inner ear and neuromast hair cells that we observed in our micrographs.
We also noted blebbing structures near ribbons in mutant hair cells in our micrographs. Three examples are shown in Figure S2. Protrusions or the appearance of destabilized membrane can be seen directly adjacent to the ribbon.
Mutant synj1 Hair Cell Output Is Delayed and Phase Locking Is Impaired
In order to address whether the disruption in SV recycling affects the timing of hair-cell transmission to PLLg neurons, we examined spike timing in response to stimulation. First, for both wild-type and synj1Q296X larvae, we determined the timing of spikes relative to the start of each 60 Hz cycle for all spikes in the first and fifteenth minute of stimulation. The spike times from all larvae are represented in a histogram in Figure 6A–B. We then calculated the mean spike time for each PLLg recording (Fig 6C). In wild-type larvae, similar to the results for spike rate, there was no change in mean spike time from the first (4.5±0.3 ms, n = 7) to the fifteenth minute (4.7±0.2 ms, n = 7) of stimulation (Fig 6C). In contrast, the mean spike time was significantly delayed following 15 minutes of sustained stimulation of synj1Q296X hair cells (5.9±0.6 ms, n = 8) compared to the first minute (4.9±0.4 ms, n = 8; Figure 6C).
A, B) Histograms of 60 seconds of activity for (A) wild-type and (B) synj1Q296X afferent neurons in the first minute (yellow bars) and fifteenth minute (blue bars) of 60 Hz stimulation. The x-axis represents the length of one 60 Hz cycle in milliseconds. Curves were fit to Gaussian distributions (yellow and blue solid lines). C) Mean spike times of activity during 60 seconds of stimulation for individual wild-type and synj1Q296X mutants represented in (A). Lines connect the 1st (yellow circles) and 15th (blue circles) minute of stimulation for each larval recording. Following 15 minutes of sustained 60 Hz stimulation there was a significant delay in spike time compared to the first minute of stimulation in synj1Q296X as well as compared to wild-type after 15 minutes of stimulation.
In addition to the overall delay in synj1 mutant hair-cell response to stimulation, inspection of both the traces in Figure 4C and the histogram of mutant activity from Figure 6B revealed an impairment in the timing of release in response to the stimulus. Proper phase locking of hair-cell output to mechanical stimuli is a reflection of precisely timed transmission. In order to determine the fidelity of timing, we quantified the quality of phase locking of afferent activity by calculating its vector strength (see Methods). A vector strength (r) value of 0 implies essentially random activity, whereas a value of 1 describes perfect synchrony between stimulus and response. We determined the vector strength of 60 seconds worth of spikes in the first and fifteenth minute of stimulation in both wild-type and mutant afferent neurons. As expected, stimulus and response were tightly phase-locked in wild-type larvae at 60 Hz (1st min: r = 0.88±0.03; 15th min: r = 0.89±0.01; n = 7; Figure 7B). In contrast, synj1Q296X mutants, similar to the reduction in spike rate seen at 60 Hz, had significantly reduced vector strength in the first minute and a further reduction after fifteen minutes of sustained stimulation (1st min: 0.75±0.05; 15th min: 0.61±0.08; n = 8; Figure 7B). In addition, mutants had reduced vector strength at 20 Hz, but only after 15 minutes of stimulation (data not shown), further supporting the relationship between the reduction in spike rate and the impairment of spike timing (Figure 4B).
A) Box plot representing phase angles from the unit vectors described by all spikes in the 1st and 15th minute of 60 Hz stimulation from wild-type (black bars) and synj1Q296X (grey bars) larvae. Above the box plots is the corresponding 60 Hz stimulus cycle with phase scale in radians. B) Individual wild-type and synj1Q296X mutant vector strength (r) values for the 60 seconds of activity described in (A). Lines connect the 1st (yellow circles) and 15th (blue circles) minute of stimulation for each larval recording. Mutant vector strength was not only reduced at both time points compared to wild-type, but also from the 1st to the 15th minute of stimulation.
Delay in Return of Spontaneous Activity Following Stimulation
We define spontaneous activity as random spikes seen in the absence of mechanical stimulation of hair cells. Typically, after a period of direct stimulation, hair cells resume spontaneous output within seconds. We noted in our recordings after ceasing prolonged stimulation that mutants displayed a delay in return of spontaneous activity. To illustrate the phenomenon, we stimulated wild-type and mutant larvae at 60 Hz for 15 minutes, removed the stimulus and continued recording until greater than 300 spontaneous spikes were collected (Figure 8A). Wild-type larvae resumed spontaneous activity within 5±2 seconds (average of first spike times; n = 4; Figure 8A). In contrast, the average time for recovery of first spikes in synj1Q296X mutants was six-fold slower (31±17 seconds, n = 4; Figure 8A). Comparison of the time elapsed for the first 300 spontaneous spikes to occur in both wild-type and mutant larvae gave an approximate rate of recovery (Figure 8B). Mutants took roughly three-fold longer to generate 300 spikes, with the majority of output delayed greater than two minutes post-stimulation. To further describe the delay in recovery of spontaneous activity, we determined the spike rate in the first 60 seconds post-stimulation in both wild-type and synj1Q296X larvae (Figure 8C). While the wild-type post-stimulation rate had nearly resumed normal levels, the firing rate in synj1Q296X mutants remained near zero (mutant: 0.4±0.2 spikes/sec, n = 4; wild-type: 4.0±1.3 spikes/sec. n = 4).
A) Single continuous trace from wild-type (top) and synj1Q296X (bottom) showing the last 10 seconds of a 15-minute 60 Hz continuous stimulus (thicker portion of black scale bar) followed by the first 30 seconds of recording in the absence of stimulation (thin portion of black scale bar). Note the greater number of wild-type spikes during the 60 Hz stimulation portion of the trace. For the wild-type trace, the initial 30 seconds post-stimulation contains 200 spikes, while there are just two spikes in the mutant trace. B) Average cumulative frequency histogram of the first 300 spontaneous spikes recorded post stimulation in wild type (black line, dark shading represents error) and synj1Q296X (grey line, light shading represents error). C) Bar graph of spontaneous spike rate for wild type and synj1Q296X larvae for the 1st (blue bars) and the 15th (green bars) minute at 0 Hz and for the 1st minute following 15 minutes of stimulation at 60 Hz (white bars). Note that the wild-type spontaneous spike rate approached that of unstimulated larvae while the mutant rate was near zero.
In this article, we report three new synaptojanin1 mutant alleles in zebrafish: synj1Q296X, synj1W943X, and synj1c.188+2T>A. The first two alleles generate truncated products; the third allele is a splice-site mutation that eliminates the translation start site. We found that the zebrafish synj1 gene is highly expressed in the nervous system and retina as previously described by Van Epps , but we also observed expression in hair cells of the larval inner ear and lateral line organ. Synj1 has been implicated in both early and later steps in classical clathrin-mediated endocytosis (CME) , and one might expect that CME would occur near ribbons where the greater part of exocytosis takes place . Indeed, CME was observed in goldfish retinal bipolar neurons ,, but molecular analysis of this type of membrane retrieval has not been examined in hair cells. Alternatively, there is evidence that bulk endocytosis occurs within the vicinity of the hair-cell ribbon. Following sustained depolarizations, large endocytic profiles and cisternae, which are hallmarks of bulk endocytosis, have been observed near hair-cell ribbons . This type of membrane retrieval is especially apparent during prolonged stimulation of hair cells. We find that Synj1 function was most critical during high demands on synaptic transmission as the synj1 mutant phenotype was more severe during sustained stimulation at higher frequencies (see below). At mutant ribbon synapses there was an increase in large coated vesicles and decreased numbers of reserve pool vesicles. Apparently, the disrupted step in membrane recycling in synj1 mutant hair cells was the breakdown of endocytic structures into small synaptic vesicles. Therefore, it remains a possibility that Synj1 is involved in one or several steps of bulk endocytosis.
Lesions in synj1 also cause similar defects in central or NMJ synapses of other species. Caenorhabditis elegans (C. elegans), Drosophila melanogaster (Drosophila), and mouse Synj1 mutants have been reported to show slowed endocytosis ,,, depletion of SVs, accumulation of clathrin-coated vesicles (CCVs), and aggregation of cortical actin ,. A unique feature of the mutant zebrafish phenotype was the presence of basal blebs in hair cells, suggestive of an imbalance of exo- and endocytosis. Indeed, the dependence of the hair-cell phenotype on the presence of Cav1.3 channels suggests that exocytosis leads to blebbing near ribbons. Another unique feature was the increase in large coated vesicles, as opposed to small coated vesicles. However, an increase of endosome-like structures is also noted in C. elegans unc-26 synapses . These authors suggest that UNC-26/SYNJ may be involved in converting endosomal compartments into synaptic vesicles. Moreover, a subpopulation of large vesicles is observed among the remaining SVs in Drosophila synj1 mutant NMJ terminals, suggesting a defect in vesicle formation . Collectively, these phenotypes support the notion that Synj1 participates in multiple steps of membrane recycling.
The apparent defect in membrane recycling in mutant synj1 hair cells had the highest impact on the reserve pool—the number of vesicles was reduced by 50%. A smaller reserve pool is consistent with an impairment of bulk retrieval . We speculate that the reduction of the reserve pool at neuromast ribbons in turn resulted in fewer tethered SVs. For our TEM experiments, we used a 60 Hz whole-larval stimulation to simulate the conditions of our physiological recordings. Whether this form of stimulation replicates the direct sinusoidal waterjet stimulation remains to be determined. Nevertheless, this rate of stimulation falls into the physiological range of lateral line hair cells . Our experiments measuring the onset of spontaneous activity after prolonged stimulation suggest that spontaneous transmission in synj1Q296X mutants is fatigable and requires several minutes to fully recover. This lengthy delay in the return of spontaneous release may reflect a preference to restock the reserve pool before un-evoked SV release is allowed to resume.
Our recordings of evoked action currents in post-synaptic neurons revealed that the mutant hair-cell synaptic properties were relatively normal during short periods of low frequency (20 Hz) stimulation. The requirement for Synj1 became apparent only when high demands on transmission were made. A decrease in the rate of transmission emerged after tens of minutes of stimulation at 20 Hz and occurred without delay at a three-fold higher frequency (60 Hz). Under these conditions, we saw a 50% or greater reduction in the number of spikes. Furthermore, at the higher frequency of 60 Hz, we observed two additional phenotypes - a delay in the spike timing and an impairment of phase locking to the stimulus. Following prolonged periods of stimulation, wild-type hair cells displayed both tight phase locking and no change in the average spike response time to stimulation. In contrast, a clear delay of spiking occurred in mutant synapses with some spikes delayed by as much as 8 ms within a 60 Hz cycle. When quantified for the degree of synchrony between the response and stimulus, mutants also displayed a significant reduction in the quality of phase locking.
The defect in phase locking, combined with the decrease in tethered vesicles and reserve pool vesicles, suggests that refilling of the ribbon or formation of SVs from endocytic compartments was rate limiting in mutant hair cells. Our results support the idea that vesicle numbers are critical for temporal fidelity of SV release at ribbon synapses . Other possibilities may also explain the loss of phase locking, such as an indirect effect on the exocytic machinery. For example, prolonged stimulation could lead to diffusion or mislocalization of active zone proteins such as Cav1.3a channels, which mediate the calcium influx required for SV fusion. In our hands, labeling of hair cells stimulated for 15 minutes at 60 Hz with antibodies against Cav1.3a and Ribeye did not reveal any differences between wild type and mutant hair-cells (Sheets, L. and Nicolson, T., unpublished observations). Another possibility is a more subtle disruption of the attachment of the ribbon to the plasma membrane. While a slight displacement of the ribbon would likely delay vesicle fusion, it isn't clear how it would disrupt phase-locking fidelity. Furthermore, ribbon mislocalization was not apparent in our immunofluorescence images or TEM micrographs. Another possibility is that the third or so hair cells with basal blebs near ribbons may account for the loss of phase locking. Perhaps the destabilization of the plasma membrane in these cells leads to abnormal fusion of SVs. A further possibility is the presence of an endocytic defect on the post-synaptic side of mutant ribbon synapses. A recent study describes the failure to endocytose pHluorin-tagged α-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid (AMPA) receptors in cultured synaptojanin-KO hippocampal neurons . In this study, an increase of miniature excitatory postsynaptic currents (mEPSC) is attributed to an increase in surface exposure of AMPA receptors. An increase in exposed AMPA receptors would likely lead to an increase in spike rates. A recycling defect of AMPA receptors in synj1Q296X PLLg neurons is possible, however, rather than observing increased spontaneous or stimulus-evoked spiking, we saw a decrease in post-synaptic activity.
In actively swimming larvae, synj1 mutants suffer from an easily observable balance defect. If swirled continuously in a stream of water, the severity of the vestibular defect increases, suggesting fatigue of synaptic transmission. Such an effect on behavior is consistent with our recordings of afferent neuron activity where a decrease in synaptic ribbon function was seen during sustained stimulation. Similarly, the reduction of a robust response in the VOR test presumably resulted from fewer synaptic transmission events during stimulation of vestibular hair cells. Light tapping on a Petri dish of synj1 mutants will elicit a response, which suggests that their acoustic startle reflex is intact (data not shown). Perhaps continuous stimulation of the auditory system in synj1 mutants would also affect this acoustic startle reflex. Whether hearing is impaired in terms of sensitivity or frequency range in mutant synj1 zebrafish awaits further study.
Our results suggest that maintenance of SV pools is critical for reliable and temporally precise transmission at the hair-cell ribbon synapse. Transduction in hair cells induces the release of thousands of vesicles during strong stimulation, which poses a particular challenge to endocytic retrieval mechanisms. Based on our data, we propose that Synj1 plays a critical role in facilitating vesicle recycling and if impaired, vesicle recycling not only affects the number of vesicles released at ribbon synapses, but also the timing of release.
Materials and Methods
Mutant alleles were maintained in Tübingen or Top Long Fin wild-type backgrounds. The transgenic lines Tg(brn3c∶mGFP) and Tg(neurod∶GFP) were previously described ,. In addition, the gemini/cav1.3R1250X (tc123d allele) mutant was previously described . The cdh23-MO (targeted to the ATG start site) was also previously described . For all experiments, the genotype of larvae was confirmed.
VOR Experiments and Analyses
Larvae were tested as previously described with some modifications . Zebrafish larvae placed in 2% low-melting agarose dorsal side up on a cover glass were mounted facedown on a vertical sample platform. The platform was driven by a motor (BE2310J-NPSN, Parker Hann. Inc.), controlled by a servo controller (GV6K-U3E, Parker Hann. Inc.), at a frequency of 30°/s and amplitude of ±60°. Movement was controlled by Motion Planner software (Parker Hann. Inc). Eye movements were recorded by digital camera (DCM130; Hangzhou Scopetek Opto-Electric, Zhejiang, China) through a 10× objective lens (Mitutoyo, Neuss, Germany), illuminated by infrared (800 nm), at 7–9 frames per second and at a resolution of 1024×768 dpi (ScopePhoto). In order to read platform position directly from video, a servo controller generated 100 ms pulse, timed 100 ms after the motor reached an edge, briefly shut down the illumination. Video was processed in MATLAB (Mathworks, Natick, MA), using functions in the Image Processing Toolbox. The ratio (R) of the long and short axes of the fish eye was extracted from each frame. For quantifying eye movement, the relative ratio change was calculated as (R – mean(R))/mean(R). Since the eye movement represented a response to sinusoidal stimulation, the magnitude of eye movement was the power at the stimulation frequency (determined by discrete Fourier transformation).
Genomic DNA and mRNA were extracted from day 5 zebrafish larvae by standard methods. First-strand cDNA was generated with the SuperScript III First-Strand Synthesis System (Invitrogen) and an oligo d(T) primer, and the alleles are designated as follows: synj1c188+2T>A (formerly called IG459), donor splice site, exon2; synj1Q296X (formerly called JV039), post sac1-domain truncation; synj1W943X (formerly called synj1HT039) post IPPc domain truncation. Primers used for sequencing were previously described .
Neuromast hair-cell cores were obtained using the same glass pipet used for hair-cell stimulation filled with 5 µl of lysis buffer. The pipet was attached via tubing to a hand-held 60 mL syringe. Upon positioning the pipet against a rosette of hair cells, gentle suction was applied while at the same time raising the pipet away from the larva. The entire hair-cell group was usually collected within seconds. On average 5 neuromasts (∼50 hair cells) were pooled, transferred to a PCR tube and then cDNA extraction and amplification was performed.
In Situ Hybridization
Digoxygenin-labeled riboprobes were generated with the DIG Labeling Kit (Roche) from synj1 (nt 1–692) cDNA that had been cloned into pCR-II (Invitrogen). In situ hybridization was conducted as previously described with 200 ng of probe . Larvae at 72 hours post fertilization were imaged on a Zeiss Axio bright-field microscope using a 10× or 20× dry lens objective. Images were acquired via an AxioCam MRc5 color digital camera using Axiovision software and exported as TIFF files to Adobe Photoshop for analysis.
Hair cells were labeled with either anti-Ribeye b or anti-Vglut3 antibodies as previously described .
Electron Microscopy and Vesicle/Endosome Counting
Mutant synj1 and wild-type larvae were stimulated on a mini-shaker (Bruel and Kjaer) at 0.5 V at 60 Hz for 15 minutes . Larvae were fixed immediately in 3% glutaraldehyde and 1.5% paraformaldehyde in 0.1 M phosphate buffer for ≥12 h, stained with 1% osmium, dehydrated in ethanol and embedded in araldite. Transverse thin sections (60–80 nm) through the ear and neuromasts were imaged on a Phillips CM100 Electron Microscope. Images of ear and neuromast hair-cell ribbons were collected at 25,000×–64,000× magnification. Images were acquired on an analog camera and negatives were scanned into Adobe Photoshop at 1200 dpi. Sections of stimulated larvae were collected from a total of 5 wild-type and 5 mutant specimens. We generated ≥5 sections per specimen, and each section contained ≥3 hair cells. Our analysis was confined to those ribbons that were directly adjacent to a plasma membrane connected to an afferent bouton. Tethered synaptic vesicles (30–50 nm diameter) were counted as described previously . Same-sized vesicles that were not tethered were counted as reserve pool vesicles. To examine the same cross-sectional area in each section (cut through the center of ribbons; off-center sections were not counted), a circle with a radius of 450 nm was created using ImageJ (NIH) and centered on the ribbon body in images at the same magnification (64,000×). We chose 450 nm because longer distances often included large mitochondria or nuclei within the cross-sectional area.
Electrophysiology and Postsynaptic Recordings
The recording setup was similar to that described previously . Briefly, day 5 larvae were anesthetized, mounted and microinjected in the heart with 125 µM α-bungarotoxin to suppress muscle activity. Larvae were then rinsed and maintained in normal extracellular solution (in mM: 120 NaCl, 2 KCl, 2 CaCl2, 1 MgCl2 and 10 HEPES, pH 7.3). The recording micropipette was also filled with normal extracellular solution. Pipettes were fabricated (P-97, Sutter Instruments) from borosilicate glass with tip diameters of approximately 1 µm and resistances between 15 to 20 MΩ. Signals were collected with an Axopatch 200B, a Digidata 1440A and pClamp 10 software (Molecular Devices). Extracellular currents were collected in voltage clamp mode, filtered at 1 kHz and sampled at 100 µs/pt. The recording electrode and waterjet pipet were positioned with stepper-motor micromanipulators (Sutter Instruments). The waterjet pipet was positioned ∼100 µm from a given neuromast and displacement of the cupula was verified by eye. The recording electrode was positioned within the posterior lateral line ganglion against a cell body of choice. Following observation of spontaneous spiking, the corresponding neuromast was located by detection of phase-locked spiking upon waterjet stimulation. The microphonic recordings were obtained as in .
Neuromast hair cell stimulation was performed as described previously . In short, we stimulated hair cells with a pressure clamp (HSPC-1, ALA Scientific, New York) attached to a glass micropipette (tip diameter ∼30 µM) filled with normal extracellular solution. In order to elicit activation of hair-cell transduction channels, the waterjet was oriented along the long body axis in order to deflect the cupula laterally. The pressure clamp was driven by a sinusoidal voltage command, which resulted in bidirectional deflection of the cupula. For a given experiment, the voltage command was driven concurrently by pClamp via a second analog output from the Digidata. During each experiment the quality and timing of the waterjet pressure was monitored via a feedback sensor located on the HSPC-1 headstage. For each experiment, this feedback pressure signal was collected in Clampex alongside the extracellular current recording and used for alignment of the apparent stimulus phase to recorded postsynaptic currents. Stimulus trains of 0, 20 and 60 Hz were delivered in 1-second episodes with minimum time between episodes.
Data were analyzed and plotted with Axograph X (Axograph Scientific) and Prism 5 (Graphpad) software. The number of spikes per second was averaged from the total number of spikes in 60 consecutive episodes. Individual spike time was calculated to correspond with each stimulus sine-wave cycle (i.e. t = 0 at the start of each sine-wave period). This resulted in every spike time occurring within 0 and either 50 (20 Hz) or 16.66 (60 Hz) milliseconds. To quantify the synchronization of the hair cell response to stimulus phase, we converted the individual spike times to unit vectors with specific phase angles (see Figure 7A). The equation for vector strength (r) was then used as a measure of the degree of phase locking between stimulus and response . Values in the text are expressed as mean±SEM. Statistical significance was determined by using either paired or unpaired Student's t tests, as appropriate, and are indicated in figures as * p<0.05, ** p<0.01, and *** p<0.001.
Synaptic ribbons in synj1Q296X hair cells are localized to basolateral regions adjacent to lateral line nerve fibers. Ribbons in wild-type (A) and synj1Q296X (B) neuromasts were labeled with anti-Ribeye b antibody (red staining). Nerve fibers were visualized using GFP fluorescence stably expressed by a neurod promoter (green staining). Shown are maximum projections of confocal sections from a top down view. Scale bar, 3 µm.
(1.65 MB TIF)
Examples of loss of integrity of the plasma membrane near mutant synj1Q296X ribbons. (A) Confocal optical sections of inner ear hair cells labeled with anti-Vglut3 antibody in wild type and synj1Q296X larvae. (B) Left panel, blebbing seen in an synj1Q296X inner ear hair cell at the TEM level. Right panels, two ribbons in synj1Q296X neuromast hair cells. Arrows indicate membrane protrusions or areas that appear to the disintegrating. Scale bar, 125 nm in left panel; 100 nm in top right panel; 200 nm in lower right panel.
(0.19 MB JPG)
Microphonic potentials are normal in synj1Q296X mutants. (A) Upper panel shows a representative wild-type microphonic recording in response to 20 Hz stimulation. The trace is an average of 200 consecutive sweeps. Lower panel shows a representative average trace from mutant following 15 minutes of sustained 60 Hz stimulation of the same neuromast. (B) The peak to peak magnitude of the microphonics were obtained from individual larvae identified in the parentheses above each condition. Mutants and mutants following stimulation were not significantly different than wild type.
(0.60 MB TIF)
We thank Stephanie Kaech-Petri and Jacqueline DeGagne for their technical assistance with the confocal and electron microscopy, and Peter Mayinger for his comments on the manuscript. We also thank the Tübingen 2000 Screen Consortium for making this work possible.
Conceived and designed the experiments: JGT NO WM TN. Performed the experiments: JGT NO WM. Analyzed the data: JGT NO WM TN. Contributed reagents/materials/analysis tools: JGT NO WM SEB. Wrote the paper: JGT NO TN.
- 1. Nouvian R, Beutner D, Parsons TD, Moser T (2006) Structure and function of the hair cell ribbon synapse. J Membr Biol 209: 153–165.R. NouvianD. BeutnerTD ParsonsT. Moser2006Structure and function of the hair cell ribbon synapse.J Membr Biol209153165
- 2. Beutner D, Moser T (2001) The presynaptic function of mouse cochlear inner hair cells during development of hearing. J Neurosci 21: 4593–4599.D. BeutnerT. Moser2001The presynaptic function of mouse cochlear inner hair cells during development of hearing.J Neurosci2145934599
- 3. Khimich D, Nouvian R, Pujol R, Tom Dieck S, Egner A, et al. (2005) Hair cell synaptic ribbons are essential for synchronous auditory signalling. Nature 434: 889–894.D. KhimichR. NouvianR. PujolS. Tom DieckA. Egner2005Hair cell synaptic ribbons are essential for synchronous auditory signalling.Nature434889894
- 4. Johnson SL, Forge A, Knipper M, Münkner S, Marcotti W (2008) Tonotopic variation in the calcium dependence of neurotransmitter release and vesicle pool replenishment at mammalian auditory ribbon synapses. J Neurosci 28: 7670–7678.SL JohnsonA. ForgeM. KnipperS. MünknerW. Marcotti2008Tonotopic variation in the calcium dependence of neurotransmitter release and vesicle pool replenishment at mammalian auditory ribbon synapses.J Neurosci2876707678
- 5. Moser T, Beutner D (2000) Kinetics of exocytosis and endocytosis at the cochlear inner hair cell afferent synapse of the mouse. Proc Natl Acad Sci U S A 97: 883–888.T. MoserD. Beutner2000Kinetics of exocytosis and endocytosis at the cochlear inner hair cell afferent synapse of the mouse.Proc Natl Acad Sci U S A97883888
- 6. Griesinger CB, Richards CD, Ashmore JF (2005) Fast vesicle replenishment allows indefatigable signalling at the first auditory synapse. Nature 435: 212–215.CB GriesingerCD RichardsJF Ashmore2005Fast vesicle replenishment allows indefatigable signalling at the first auditory synapse.Nature435212215
- 7. Lenzi D, Runyeon JW, Crum J, Ellisman MH, Roberts WM (1999) Synaptic vesicle populations in saccular hair cells reconstructed by electron tomography. J Neurosci 19: 119–132.D. LenziJW RunyeonJ. CrumMH EllismanWM Roberts1999Synaptic vesicle populations in saccular hair cells reconstructed by electron tomography.J Neurosci19119132
- 8. Fuchs PA (2005) Time and intensity coding at the hair cell's ribbon synapse. J Physiol 566: 7–12.PA Fuchs2005Time and intensity coding at the hair cell's ribbon synapse.J Physiol566712
- 9. Moser T, Neef A, Khimich D (2006) Mechanisms underlying the temporal precision of sound coding at the inner hair cell ribbon synapse. J Physiol 576: 55–62.T. MoserA. NeefD. Khimich2006Mechanisms underlying the temporal precision of sound coding at the inner hair cell ribbon synapse.J Physiol5765562
- 10. Goutman JD, Glowatzki E (2007) Time course and calcium dependence of transmitter release at a single ribbon synapse. Proc Natl Acad Sci U S A 104: 16341–16346.JD GoutmanE. Glowatzki2007Time course and calcium dependence of transmitter release at a single ribbon synapse.Proc Natl Acad Sci U S A1041634116346
- 11. Wittig JH Jr, Parsons TD (2008) Synaptic ribbon enables temporal precision of hair cell afferent synapse by increasing the number of readily releasable vesicles: a modeling study. J Neurophysiol 100: 1724–1739.JH Wittig JrTD Parsons2008Synaptic ribbon enables temporal precision of hair cell afferent synapse by increasing the number of readily releasable vesicles: a modeling study.J Neurophysiol10017241739
- 12. Lenzi D, Crum J, Ellisman MH, Roberts WM (2002) Depolarization redistributes synaptic membrane and creates a gradient of vesicles on the synaptic body at a ribbon synapse. Neuron 36: 649–659.D. LenziJ. CrumMH EllismanWM Roberts2002Depolarization redistributes synaptic membrane and creates a gradient of vesicles on the synaptic body at a ribbon synapse.Neuron36649659
- 13. Schnee ME, Lawton DM, Furness DN, Benke TA, Ricci AJ (2005) Auditory hair cell-afferent fiber synapses are specialized to operate at their best frequencies. Neuron 47: 243–254.ME SchneeDM LawtonDN FurnessTA BenkeAJ Ricci2005Auditory hair cell-afferent fiber synapses are specialized to operate at their best frequencies.Neuron47243254
- 14. Dickman DK, Horne JA, Meinertzhagen IA, Schwarz TL (2005) A slowed classical pathway rather than kiss-and-run mediates endocytosis at synapses lacking synaptojanin and endophilin. Cell 123: 521–533.DK DickmanJA HorneIA MeinertzhagenTL Schwarz2005A slowed classical pathway rather than kiss-and-run mediates endocytosis at synapses lacking synaptojanin and endophilin.Cell123521533
- 15. Cremona O, De Camilli P (2001) Phosphoinositides in membrane traffic at the synapse. J Cell Sci 114: 1041–1052.O. CremonaP. De Camilli2001Phosphoinositides in membrane traffic at the synapse.J Cell Sci11410411052
- 16. Cremona O, Di Paolo G, Wenk MR, Lüthi A, Kim WT, et al. (1999) Essential role of phosphoinositide metabolism in synaptic vesicle recycling. Cell 99: 179–188.O. CremonaG. Di PaoloMR WenkA. LüthiWT Kim1999Essential role of phosphoinositide metabolism in synaptic vesicle recycling.Cell99179188
- 17. Kim CH, Hwang DY, Park JJ, Kim KS (2002) A proximal promoter domain containing a homeodomain-binding core motif interacts with multiple transcription factors, including HoxA5 and Phox2 proteins, and critically regulates cell type-specific transcription of the human norepinephrine transporter gene. J Neurosci 22: 2579–2589.CH KimDY HwangJJ ParkKS Kim2002A proximal promoter domain containing a homeodomain-binding core motif interacts with multiple transcription factors, including HoxA5 and Phox2 proteins, and critically regulates cell type-specific transcription of the human norepinephrine transporter gene.J Neurosci2225792589
- 18. Allwardt BA, Lall AB, Brockerhoff SE, Dowling JE (2001) Synapse formation is arrested in retinal photoreceptors of the zebrafish nrc mutant. J Neurosci 21: 2330–2342.BA AllwardtAB LallSE BrockerhoffJE Dowling2001Synapse formation is arrested in retinal photoreceptors of the zebrafish nrc mutant.J Neurosci2123302342
- 19. Van Epps HA, Hayashi M, Lucast L, Stearns GW, Hurley JB, et al. (2004) The zebrafish nrc mutant reveals a role for the polyphosphoinositide phosphatase synaptojanin 1 in cone photoreceptor ribbon anchoring. J Neurosci 24: 8641–8650.HA Van EppsM. HayashiL. LucastGW StearnsJB Hurley2004The zebrafish nrc mutant reveals a role for the polyphosphoinositide phosphatase synaptojanin 1 in cone photoreceptor ribbon anchoring.J Neurosci2486418650
- 20. McDermott BM, Baucom JM, Hudspeth AJ (2007) Analysis and functional evaluation of the hair-cell transcriptome. Proc Natl Acad Sci U S A 104: 11820–11825.BM McDermottJM BaucomAJ Hudspeth2007Analysis and functional evaluation of the hair-cell transcriptome.Proc Natl Acad Sci U S A1041182011825
- 21. Wang C, Du XN, Jia QZ, Zhang HL (2005) Binding of PLCdelta1PH-GFP to PtdIns(4,5)P2 prevents inhibition of phospholipase C-mediated hydrolysis of PtdIns(4,5)P2 by neomycin. Acta Pharmacol Sin 26: 1485–1491.C. WangXN DuQZ JiaHL Zhang2005Binding of PLCdelta1PH-GFP to PtdIns(4,5)P2 prevents inhibition of phospholipase C-mediated hydrolysis of PtdIns(4,5)P2 by neomycin.Acta Pharmacol Sin2614851491
- 22. Sidi S, Busch-Nentwich E, Friedrich R, Schoenberger U, Nicolson T (2004) gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapses. J Neurosci 24: 4213–4223.S. SidiE. Busch-NentwichR. FriedrichU. SchoenbergerT. Nicolson2004gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapses.J Neurosci2442134223
- 23. Brandt A, Khimich D, Moser T (2005) Few CaV1.3 channels regulate the exocytosis of a synaptic vesicle at the hair cell ribbon synapse. J Neurosci 25: 11577–11585.A. BrandtD. KhimichT. Moser2005Few CaV1.3 channels regulate the exocytosis of a synaptic vesicle at the hair cell ribbon synapse.J Neurosci251157711585
- 24. Nicolson T, Rüsch A, Friedrich RW, Granato M, Ruppersberg JP, Nüsslein-Volhard C (1998) Genetic analysis of vertebrate sensory hair cell mechanosensation: the zebrafish circler mutants. Neuron 20: 271–283.T. NicolsonA. RüschRW FriedrichM. GranatoJP RuppersbergC. Nüsslein-Volhard1998Genetic analysis of vertebrate sensory hair cell mechanosensation: the zebrafish circler mutants.Neuron20271283
- 25. Söllner C, Rauch GJ, Siemens J, Geisler R, Schuster SC, et al. (2004) Mutations in cadherin 23 affect tip links in zebrafish sensory hair cells. Nature 428: 955–959.C. SöllnerGJ RauchJ. SiemensR. GeislerSC Schuster2004Mutations in cadherin 23 affect tip links in zebrafish sensory hair cells.Nature428955959
- 26. Obholzer N, Wolfson S, Trapani JG, Mo W, Nechiporuk A, et al. (2008) Vesicular glutamate transporter 3 is required for synaptic transmission in zebrafish hair cells. J Neurosci 28: 2110–2118.N. ObholzerS. WolfsonJG TrapaniW. MoA. Nechiporuk2008Vesicular glutamate transporter 3 is required for synaptic transmission in zebrafish hair cells.J Neurosci2821102118
- 27. Bleckmann H (2008) Peripheral and central processing of lateral line information. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 194: 145–158.H. Bleckmann2008Peripheral and central processing of lateral line information.J Comp Physiol A Neuroethol Sens Neural Behav Physiol194145158
- 28. Harris TW, Hartwieg E, Horvitz HR, Jorgensen EM (2000) Mutations in synaptojanin disrupt synaptic vesicle recycling. J Cell Biol 150: 589–600.TW HarrisE. HartwiegHR HorvitzEM Jorgensen2000Mutations in synaptojanin disrupt synaptic vesicle recycling.J Cell Biol150589600
- 29. Zeddies DG, Fay RR (2005) Development of the acoustically evoked behavioral response in zebrafish to pure tones. J Exp Biol 208: 1363–1372.DG ZeddiesRR Fay2005Development of the acoustically evoked behavioral response in zebrafish to pure tones.J Exp Biol20813631372
- 30. Perera RM, Zoncu R, Lucast L, De Camilli P, Toomre D (2006) Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Proc Natl Acad Sci U S A 103: 19332–19337.RM PereraR. ZoncuL. LucastP. De CamilliD. Toomre2006Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages.Proc Natl Acad Sci U S A1031933219337
- 31. Zenisek D (2008) Vesicle association and exocytosis at ribbon and extraribbon sites in retinal bipolar cell presynaptic terminals. Proc Natl Acad Sci U S A 105: 4922–4927.D. Zenisek2008Vesicle association and exocytosis at ribbon and extraribbon sites in retinal bipolar cell presynaptic terminals.Proc Natl Acad Sci U S A10549224927
- 32. Jockusch WJ, Praefcke GJ, McMahon HT, Lagnado L (2005) Clathrin-dependent and clathrin-independent retrieval of synaptic vesicles in retinal bipolar cells. Neuron 46: 869–878.WJ JockuschGJ PraefckeHT McMahonL. Lagnado2005Clathrin-dependent and clathrin-independent retrieval of synaptic vesicles in retinal bipolar cells.Neuron46869878
- 33. Park SJ, Lee DS, Lim EJ, Choi SH, Kang WS, et al. (2008) The absence of the clathrin-dependent endocytosis in rod bipolar cells of the FVB/N mouse retina. Neurosci Lett 439: 165–169.SJ ParkDS LeeEJ LimSH ChoiWS Kang2008The absence of the clathrin-dependent endocytosis in rod bipolar cells of the FVB/N mouse retina.Neurosci Lett439165169
- 34. Verstreken P, Koh TW, Schulze KL, Zhai RG, Hiesinger PR, et al. (2003) Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating. Neuron 40: 733–748.P. VerstrekenTW KohKL SchulzeRG ZhaiPR Hiesinger2003Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating.Neuron40733748
- 35. Mani M, Lee SY, Lucast L, Cremona O, Di Paolo G, et al. (2007) The dual phosphatase activity of synaptojanin1 is required for both efficient synaptic vesicle endocytosis and reavailability at nerve terminals. Neuron 56: 1004–1018.M. ManiSY LeeL. LucastO. CremonaG. Di Paolo2007The dual phosphatase activity of synaptojanin1 is required for both efficient synaptic vesicle endocytosis and reavailability at nerve terminals.Neuron5610041018
- 36. Matthews G (2004) Cycling the synapse: scenic versus direct routes for vesicles. Neuron 44: 223–226.G. Matthews2004Cycling the synapse: scenic versus direct routes for vesicles.Neuron44223226
- 37. Gong LW, De Camilli P (2008) Regulation of postsynaptic AMPA responses by synaptojanin 1. Proc Natl Acad Sci U S A 105: 17561–17566.LW GongP. De Camilli2008Regulation of postsynaptic AMPA responses by synaptojanin 1.Proc Natl Acad Sci U S A1051756117566
- 38. Xiao T, Roeser T, Staub W, Baier H (2005) A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection. Development 132: 2955–2967.T. XiaoT. RoeserW. StaubH. Baier2005A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection.Development13229552967
- 39. Goldberg JM, Brown PB (1969) Response of binaural neurons of dog superior olivary complex to dichotic tonal stimuli: some physiological mechanisms of sound localization. J Neurophysiol 32: 613–636.JM GoldbergPB Brown1969Response of binaural neurons of dog superior olivary complex to dichotic tonal stimuli: some physiological mechanisms of sound localization.J Neurophysiol32613636