The majority of the genome in animals and plants is transcribed in a developmentally regulated manner to produce large numbers of non–protein-coding RNAs (ncRNAs), whose incidence increases with developmental complexity. There is growing evidence that these transcripts are functional, particularly in the regulation of epigenetic processes, leading to the suggestion that they compose a hitherto hidden layer of genomic programming in humans and other complex organisms. However, to date, very few have been identified in genetic screens. Here I show that this is explicable by an historic emphasis, both phenotypically and technically, on mutations in protein-coding sequences, and by presumptions about the nature of regulatory mutations. Most variations in regulatory sequences produce relatively subtle phenotypic changes, in contrast to mutations in protein-coding sequences that frequently cause catastrophic component failure. Until recently, most mapping projects have focused on protein-coding sequences, and the limited number of identified regulatory mutations have been interpreted as affecting conventional cis-acting promoter and enhancer elements, although these regions are often themselves transcribed. Moreover, ncRNA-directed regulatory circuits underpin most, if not all, complex genetic phenomena in eukaryotes, including RNA interference-related processes such as transcriptional and post-transcriptional gene silencing, position effect variegation, hybrid dysgenesis, chromosome dosage compensation, parental imprinting and allelic exclusion, paramutation, and possibly transvection and transinduction. The next frontier is the identification and functional characterization of the myriad sequence variations that influence quantitative traits, disease susceptibility, and other complex characteristics, which are being shown by genome-wide association studies to lie mostly in noncoding, presumably regulatory, regions. There is every possibility that many of these variations will alter the interactions between regulatory RNAs and their targets, a prospect that should be borne in mind in future functional analyses.
Citation: Mattick JS (2009) The Genetic Signatures of Noncoding RNAs. PLoS Genet 5(4): e1000459. https://doi.org/10.1371/journal.pgen.1000459
Editor: Jeannie T. Lee, Massachusetts General Hospital, Howard Hughes Medical Institute, United States of America
Published: April 24, 2009
Copyright: © 2009 John S. Mattick. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The work was supported by the Australian Research Council (Fellowship Grant FF0561986), the University of Queensland, and the Queensland State Government. These agencies played no role in the preparation, review, or approval of the manuscript.
Competing interests: The author has declared that no competing interests exist.
Genome sequencing projects have shown that the numbers of protein-coding genes and the extent of protein-coding sequences do not change appreciably across the vertebrates nor indeed across the metazoa as a whole, despite large differences in developmental complexity . On the other hand, the extent of non–protein-coding intronic and intergenic sequences in genomes does increase with developmental complexity, suggesting that these sequences may contain increasingly elaborate regulatory information .
In recent years it has also become evident that the vast majority of the mammalian genome, and that of other complex organisms, is transcribed, apparently in a developmentally regulated manner, to produce large numbers of ncRNAs that are antisense, intergenic, interleaved, or overlapping with protein-coding genes –. In addition, there are increasing reports (Figure 1) of the functionality of individual ncRNAs in mammals (Table 1), other animals (see, e.g., –), plants (e.g., ,), and fungi (e.g., ), particularly in relation to developmental processes . These include the involvement of ncRNAs in the regulation of the expression of homeotic genes ,, oncogenes , and metabolic genes , as well as in the regulation of skeletal development , eye development , epithelial-to-mesenchymal transition , and subcellular structures –, among many others (for reviews and additional examples, see , , –).
The number of indexed Medline entries with the words “non-coding RNA”, “noncoding RNA”, “non-protein-coding RNA” or “ncRNA” in the title, abstract or keywords is plotted per year. Data courtesy of Ryan J. Taft.
There is also compelling genome-wide evidence that the large numbers of identified but as yet unstudied intronic, intergenic, and antisense ncRNAs have intrinsic indices of functionality (Table 2), as indicated by the following: (i) the conservation of their promoters, splice junctions, exons, predicted structures, genomic position, and expression patterns , –; (ii) their dynamic expression and alternative splicing during differentiation ,,; (iii) their altered expression or splicing patterns in cancer and other diseases –; (iv) their association with particular chromatin signatures that are indicative of actively transcribed genes ,; (v) their regulation by key morphogens and transcription factors ,,,; and (vi) their tissue- and cell-specific expression patterns and subcellular localization , , –, , –.
Indeed, a recent study of over 1,300 mouse ncRNAs showed that almost half of them exhibit precise expression patterns in different parts of the brain, including different subregions of the hippocampus, olfactory bulb, neocortex, and cerebellum . While many ncRNAs, like other regulatory sequences (see below), appear to be evolving quickly, including those with well-validated functions , most retain conserved patches within them ,, and some may be under positive selection, especially in the brain . At least some of these RNAs, including intronic RNAs, are precursors for small regulatory RNAs like microRNAs (miRNAs), small interfering RNAs (siRNAs), piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs) –. Many miRNAs are highly conserved from nematodes to humans, whereas others are primate-specific, and their full repertoire remains to be determined ,,.
It is widely accepted that animals have a relatively common set of protein-coding genes and that, notwithstanding lineage-specific innovations and splice variants, the primary basis of phenotypic, especially morphological, radiation and higher complexity has been the variation and expansion of the regulatory architecture that controls the deployment of these protein components and their isoforms during differentiation and development . This regulatory architecture is generally more plastic than protein-coding sequences that are highly constrained by relatively strict structure-function relationships, which is reflected by the fact that regulatory sequences evolve at widely different rates –. These sequences range from promoter regions that have no recognizable sequence similarity yet direct orthologous patterns of gene expression between fishes and mammals  to highly conserved non-genic elements ,, and “ultraconserved” sequences that have remained essentially unchanged over hundreds of millions of years of vertebrate evolution and appear to act as tissue-specific enhancers that regulate gene expression during development –.
Regulatory sequences are also generally assumed to operate through their interactions with sequence-specific transcription factors and other regulatory proteins, but this assumption has been made in ignorance, until recently, of the extent of developmentally regulated transcription of ncRNAs from the genome, including many regions spanning enhancers and promoters (see, e.g., , –). The possibility is therefore that the genomes of mammals and other complex organisms encode a large repertoire of regulatory RNAs . Indeed, the case has been made that a much higher degree of regulatory sophistication, aided by the co-option of the considerable powers of RNA to transmit sequence-specific information, was a prerequisite for the evolution of developmentally complex organisms ,, and that many of these RNAs may be involved in the regulation of developmental processes, including the epigenetic trajectories that underpin them, for which there is increasing evidence ,.
However, if these ncRNAs are functional and important in developmental and physiological processes, why have so few been identified in genetic screens to date? Here I outline the emerging evidence for ncRNA involvement in key molecular genetic phenomena and in specific functions and phenotypes. I also outline the expectational, perceptual, and practical factors that may collectively account for the low genetic visibility of individual ncRNAs. Awareness of these factors and the possibility that the structure of the genomic programming of complex organisms is different from our current understanding may lead to the increased recognition of ncRNAs in genetic analyses, assisted by the emerging fusion of genetics, genomics, and systems biology.
The ability to detect a relevant mutation or variation is dependent on the sensitivity of the phenotypic screen. Mutations in protein-coding sequences usually give severely compromised (i.e., obvious) phenotypes, whereas those in regulatory sequences often do not. Proteins are the key structural and functional analogue components of cells, and the loss of their function is often disastrous, leading in many cases to obvious defects, and in some cases to embryonic lethality. Mutations in generic transcription factors and other “regulatory” proteins are included, and their loss causes pleiotropic effects on gene expression at many loci and plays an important role in the molecular etiology of cancer ,,. This is in contrast to regulatory sequences, which, when damaged, may only affect a small part of the network, with more restricted and subtle consequences, often referred to as quantitative trait variations. Indeed the use of the word “mutation”, as opposed to “variation”, reflects an inherent bias in the identification of genetic factors that influence phenotype in animals, with those exhibiting strong effects understandably having taken precedence over those that do not, both perceptually and practically. Consistent with this, until recently, there were relatively few regulatory mutations identified among the catalogue of known human mutations that are associated with overt genetic disease.
There is, of course, a wide spectrum of effects of both coding and noncoding mutations (Figure 2), and there are exceptions to the rule in both directions. Loss-of-function mutations in some protein-coding genes have mild effects , as exemplified by as knockouts of the mammalian genes encoding calbindin D9k  and C/EBPdelta ,, and the significant number of yeast genes that show no observable phenotype. Reciprocally, knockouts of some highly conserved miRNAs, many of which have multiple targets, give strong phenotypes –, even though very few such genes have been identified in genetic screens in Caenorhabditis elegans and Drosophila, and none have been identified in mice, despite the intensity of such screens (see below). Moreover, to date, no naturally arising mutations have been discovered in the Xist gene in either humans or mice, despite the central role that this ncRNA plays in embryogenesis and in X-chromosome dosage control in females , possibly because such mutations are lethal.
A conceptual diagram of the spectrum of phenotypic effects of mutations in sequences encoding proteins and other analogue components of cells (continuous line) versus variations in non-coding sequences that specify regulatory interactions (dashed line).
On the other hand, the potential subtlety of mutations in ncRNAs, even when they are targeted by reverse genetics, is illustrated by the case of BC1, which is expressed in synaptodendritic domains of neurons in rodents. Knockout of this transcript produces no obvious physical or neurological abnormality, and the mutant mice were initially indistinguishable from wild type, but were subsequently found to have reduced exploratory behaviour and consequently a higher mortality in field experiments . Thus this ncRNA causes a subtle behavioural phenotype that is invisible in the cage, and would escape detection in superficial forward genetic screens, but is almost certainly strongly disadvantageous in the wild. Similarly, deletions or insertions in some ultraconserved enhancers yield no discernable abnormality ,, despite the fact that these sequences are clearly under intense selection , suggesting insensitivity of phenotypic screens in captivity or redundancy in the regulatory architecture (perhaps associated with developmental robustness) that we have yet to understand.
Monogenic Diseases and High Penetrance Mutations
The generally stronger effects of protein-coding mutations leads to a sampling bias, in that more severe phenotypes are not only more easily discerned but have also been more likely to attract further study, both in medical contexts and in model organisms. In medicine, mutations involving catastrophic component damage have been traditionally referred to as “monogenic diseases” and were the primary targets of study in the pioneering days of human genomics, just a decade or two ago, when the protein-coding genes underpinning cystic fibrosis, Huntington disease, and Duchenne muscular dystrophy, among others, were identified by positional mapping and cloning approaches. Thus, in humans, mutation mapping is still both a young science and extraordinarily difficult due to the sheer complexity of the genome, which naturally led to an initial focus on severe loss-of-function diseases that exhibit simple inheritance patterns with high penetrance (“single gene–large effect”) making them amenable to identification. This is now changing with the increasing availability of human genome sequences, and the application of genome-wide association (GWA) studies to the mapping of the genetic components of complex traits, wherein lies the majority of the health burden and the majority of the interesting phenotypic differences between individuals and species.
Complex Traits and GWA Studies
Indeed, functional classification of sequence variations that have been identified by GWA studies to be associated with complex traits, albeit still strongly focused on those of medical importance, shows that the vast majority of variations are located in noncoding regions ,. In most cases, the causative mutations have yet to be defined, and their mechanistic basis is unknown—especially whether they affect cis-acting binding sites for regulatory proteins or the function or expression of regulatory RNAs. One good candidate for the latter is the ncRNA ANRIL, which lies antisense to CDKN2A and traverses a noncoding region centromeric to CDKN2A, a region implicated in a range of complex diseases including cancer, type 2 diabetes, periodontitis, and coronary heart disease –. Perplexingly, however, those variants mapped by GWA studies to date account for only a small proportion of genetic variation in disease or quantitative traits . These traits are clearly multifactorial and may be affected by rare variants with strong effects that have yet to be recognized.
The identification of the sequence changes that directly underpin quantitative trait variations has thus far been possible, or at least achieved, only in well-structured pedigrees in plants and animals. In the few cases where such quantitative trait loci (QTLs) have been mapped to completion, most have been found to be located in noncoding sequences, specifically: (i) regulatory sequences in promoters and distal enhancers (e.g., the “teosinte branched1” mutation affecting branching and inflorescence in maize ); (ii) 3′ untranslated regions (UTRs) (e.g., those underlying Tourette's syndrome , and muscular hypertrophy in sheep ; see also below); (iii) introns (e.g., a QTL affecting muscle growth in domestic pigs ); or (iv) intergenic sequences of unknown transcriptional status (e.g., the “callipyge” mutation causing posterior muscular hypertrophy in sheep ). The latter occurs in an imprinted locus and affects the expression of a number of protein-coding and ncRNA genes  associated with an unusual genetic phenomena termed “polar overdominance” , which may also occur in humans . While these mutations are reasonably assumed to be regulatory in nature, their mechanistic basis has not been determined, although in the latter case, there is some evidence for the involvement of trans-acting miRNAs ,. In addition, linkage studies in a large family have recently identified the ncRNA AK023948 as a candidate susceptibility gene for papillary thyroid carcinoma .
Type of Mutation and Sensitivity of the Model System
The nature of the organism under study and the type of mutations also affect the outcome of genetic screens—relevant single-base mutations are not only harder to identify than insertions/deletions, especially in mammalian genomes and even in inbred mice, but also have milder effects on regulatory sequences.
Most mutations induced by ENU mutagenesis involve single-base changes, which are also, along with small indels and copy number variations, the most common type of natural variation in humans and other mammals, where few regulatory mutations have yet been identified. Whereas a nonsynonymous mutation in a protein-coding sequence can have severe effects on the structure and function of the protein, many regulatory sequences have loose consensus sequences, and variations in them, as noted already, may have subtle effects and go unnoticed, especially in superficial phenotypic screens.
On the other hand, insertions and deletions dominate mutational screens in Drosophila, and a large number map to noncoding intergenic and intronic regions. This is exemplified by the intensively studied bithorax complex, where there are not only mutations known in the coding sequences for the homeotic protein Ultrabithorax (Ubx), but also in upstream (bithoraxoid or bxd) and intronic (Contrabithorax or Cbx and anterobithorax or abx) sequences , which contain conserved blocks within them –. Such noncoding mutations are interpreted as affecting orthodox cis-acting enhancer sequences (i.e., those that bind cis-acting regulatory proteins), despite the fact that (for example) bxd mutations fall within a region that is transcribed during early embryogenesis into a complex set of short polyadenylated RNAs with no coding potential. These RNAs arise by alternative splicing of at least 11 exons derived from a 26-kb primary transcript . Moreover, these regulatory regions involve interaction with Polycomb-group and Trithorax-group proteins, which are increasingly implicated as being directed to their sites of action by ncRNAs ,, (see below).
Similarly the iab regulatory elements of the bithorax complex that control the expression of abdominal-A and Abdominal-B, and consequently the identities of the 2nd–9th abdominal segments, are transcribed into ncRNAs in a spatially ordered pattern . It has also recently been shown that 231 ncRNAs are expressed from the four human HOX loci in a spatially and temporally ordered progression along developmental axes, one of which (termed HOTAIR) from the HOXC locus controls expression of the HOXD locus in trans, as shown by siRNA-mediated knockdown experiments . None of these ncRNAs has yet been specifically associated with a genetic variant in mammals, although in Drosophila there are many homeotic mutations that lie in regions encompassed by ncRNAs ,, and Drosophila geneticists were clearly intrigued by them . Moreover, as pointed out by Rinn et al. , the existence of such transacting regulatory ncRNAs may explain the observation that the deletion of the entire HOXC locus exhibits a milder phenotype than the deletion of individual HOXC genes .
Despite the now known importance of miRNAs in the control of gene expression ,, only four miRNA loci have been identified in intense genetic screens in C. elegans and Drosophila, and none in mammals. This may be partly due to the fact that C. elegans is hermaphroditic and naturally driven to homozygosity in individual isolates, without the need for laborious back-crossing, and flies are routinely bred to homozygosity in mutational screens, making screening of recessive mutations far more efficient than in mice. There was also an element of serendipity, in that the first identified miRNA locus, lin-4, expressed a small RNA whose complementary sequence was present in multiple copies in the 3′ UTR of its target gene, lin-14, and thus was relatively easy to pinpoint ,, which also applied in the subsequent case of let-7 .
Following the discovery of miRNAs, Drosophila mutants of uncertain provenance that mapped in gain-of-function screens to noncoding regions were re-analysed, and one that regulates growth , termed bantam, was identified to encode a miRNA . The miRNAs let-7  and lsy-6  were also identified genetically in C. elegans, not in other organisms, despite the former being not only highly conserved in sequence and expression pattern throughout metazoan evolution , but also fundamental to normal and abnormal developmental processes –, as are many other miRNAs . On the other hand, lsy-6 is expressed in only a few cells , and has only rarely turned up in deep sequencing libraries. Subsequently most known miRNAs, of which there are hundreds in mammals, and later piRNAs, have been identified by biochemical not genetic means. In view of the lsy-6 example and the clearly incomplete sampling of the small RNA transcriptome, even using deep sequencing , there are likely to be many more.
Expectations and Interpretations
There has also been a strong expectation that mutations that have phenotypic effects will map to protein-coding genes or cis-regulatory elements that interact with regulatory proteins. The former has influenced the practical strategies for mutation searching, in terms of a focus on exon scanning of candidate genes (see below), and the latter has influenced the interpretation of regulatory variations, although in only a few cases has the mechanistic basis been determined ,. Some mutations map to gene “deserts” (see, e.g., ), and while it is conceivable that they affect distal enhancers (see, e.g., ), it is interesting and relevant to note that there is good evidence that enhancers and other regulatory sequences are transcribed into ncRNAs in the cells in which they are active , –, and hence may act in part via ncRNAs.
Transvection and Locally Acting ncRNAs
Many loci, such as the bithorax complex referred to earlier, exhibit a genetic phenomenon called “transvection,” whereby a wild-type regulatory region upstream of a defective protein-coding sequence on one chromosome can rescue a relatively normal phenotype, when it is combined with a mutant regulatory region linked to a wild-type protein-coding sequence on the homologous chromosome (both of which give mutant phenotypes when homozygous) . This phenomenon is well documented in Drosophila but appears to occur in most animals and has been interpreted as a physical cross-talk between functional cis-acting promoters or enhancers on one chromosome to engender transcription of adjacent protein-coding genes on the other, since the effect is usually pairing-dependent and lost when the regulatory and protein-coding sequences are separated to nonsyntenic positions in the genome ,. However, this is not always the case—at some loci, transvection between regulatory elements and protein-coding sequences can operate over large distances (even between different chromosomes) –, suggesting the involvement of a trans-acting signal. Moreover, many promoter elements that exhibit transvection are transcribed into ncRNAs, and transvection is altered in Polycomb and zeste mutants –,, indicating that epigenetic processes (which may be regulated by ncRNAs, see below) are involved. Taken together, these observations raise the possibility that transvection is mediated by trans-acting RNAs , in which case the observed cross-complementation may occur simply as a result of a compound heterozygosity between a regulatory ncRNA locus and a nearby protein-coding locus whose expression is controlled by it.
In support of this proposition, there is now rapidly emerging evidence that many ncRNAs derived from either same or opposite strands act locally to regulate the epigenetic status and expression of nearby protein-coding genes, often involving the recruitment of chromatin-activator or repressor complexes , –, , –, with sense-antisense pairs in some cases being the substrate for the generation of siRNAs –,. Moreover the many deletion studies of gene promoter regions to define regulatory sequences have almost always assumed, physically and mechanistically, that resultant changes to expression patterns are due to the loss of cis-acting protein binding sites rather than deletions in the same or opposite strand ncRNAs that frequently traverse and are expressed from the same region. The complexity of these relationships is illustrated by the examples of the ncRNA DLeu2 (deleted in lymphocytic leukemia 2), which has multiple splice variants and lies antisense to genes in a region deleted in various malignancies , and the ncRNA ANRIL referred to earlier. Thus the interpretation of the mechanisms by which such mutations operate remains not only an open question but a difficult problem to disentangle, given the complex interlacing and overlapping coding and noncoding transcripts, and splice variants thereof, that are expressed from many loci in different cells and tissues ,.
Transinduction, Ectopic Expression and Gene Knockouts
RNA is also implicated in a curious genetic phenomenon called “transinduction,” whereby transient transfection of a β-globin gene induces transcription of the “locus control region” and intergenic regions at the chromosomal β-globin locus in non-erythroid cell lines. This effect is dependent on transcription of the globin gene from the transfected plasmid and its association with the endogenous β-globin locus, but not on protein expression, and therefore is RNA-mediated , although the responsible sequences have not been mapped. Indeed, the general assumption that mRNA is simply an intermediate between gene and protein, albeit with cis-acting regulatory elements, may be incorrect, and there may be a false dichotomy between coding and noncoding RNA . This is indicated by the complexity of overlapping sense and antisense coding and noncoding transcripts from most genomic loci – and evidence that protein-coding sequences are under constraint not only at the amino acid sequence level, but also within their RNA sequence . Moreover, given that many gene knockout studies concomitantly delete potential sources of regulatory ncRNAs such as introns (given that many miRNAs and all snoRNAs are sourced from introns) and antisense transcribed sequences, all aspects of the observed phenotypes cannot be unequivocally or solely ascribed to the loss of the protein without complementation studies or more precise deletions that are rarely done for reasons of technical difficulty.
The focus on protein-coding sequences has been reinforced by a practical problem, especially in mammals. Mutation mapping studies using whole-genome scanning techniques usually have not pinpointed the causative mutation or variation, and the affected region may encompass one megabase or more. Until recently, comparative sequencing of such regions was not feasible, and in any case it can be very difficult to sort the relevant polymorphism from the considerable background variation in huge intergenic and intronic regions, especially in outbred human populations. In most circumstances, understandably, investigators have resorted to analysing the most plausible candidate genes (recently including those expressing noncoding transcripts or ESTs ,) usually involving scanning of known exons (and sometimes the immediate 5′ flanking promoter sequences and UTRs) in the region by PCR amplification, in the hope that they can identify the causative mutation in these locations, which in turn are the ones that are then reported in the literature. However, there are many informal reports of mapping studies that have not identified such exonic mutations, and which consequently lie in abeyance, including (as noted already) the large number of GWA studies that have mapped disease-associated variations to noncoding, presumably regulatory, regions . A reasonable strategy for searching for the relevant variations in these regions may be to focus on sequences that exhibit evolutionary conservation and/or whose expression is altered . Conversely, reverse genetic screens looking for phenotypes associated with ncRNAs might target conserved blocks within them and focus on tissues where they are known to be expressed.
Mutations Affecting trans-Acting Functions of mRNAs
As noted above, regulatory mutations have been identified in the 3′ UTRs of mRNAs, such as those underlying Tourette's syndrome  and muscular hypertrophy in sheep , which appear to involve gain- or loss-of-function of miRNA binding sites. Interestingly, however, a number of other reported 3′ UTR mutations do not appear to act in cis to regulate the expression of the associated mRNA, as is normally assumed, but rather in trans as ncRNAs. For example the 3′ UTR of prohibitin (in the absence of the associated protein-coding sequences) can inhibit cell cycle progression in one complementation group of breast cancer–derived cells that is characterized by naturally occurring mutations in the 3′ UTR, indicating that these sequences are in fact functioning, in part, as trans-acting ncRNAs ,. Similarly, the oogenesis defect observed in Drosophila oskar null mutants is rescued by the oskar 3′ UTR alone .
A trans-acting function for mRNA sequences, both coding and noncoding, may be more general that expected. For example, the introduction of cancer-associated silent point-mutations in p53 mRNA alters its binding to the protein Mdm2, which in turn alters p53 expression and function . The 3′ UTRs of troponin I, tropomyosin, and α-cardiac actin have been shown to reactivate muscle-specific promoters in a differentiation-defective myoblast mutant, enhance the differentiation of wild-type muscle cells, and suppress the proliferation of fibroblasts independently of their normally associated protein-coding sequences . Similarly, the 3′ UTRs of tropomysin  and ribonucleotide reductase  can suppress tumour formation, and the 3′ UTR of the DM protein kinase gene, which is involved in myotonic dystrophy, inhibits the differentiation of C2C12 myoblasts . Moreover, many 3′ UTRs in mouse appear to be expressed separately from their mRNAs in a developmentally regulated manner .
There other examples of mutations in sequences encoding 3′ UTRs that do not act via the UTR. A single nucleotide polymorphism that determines susceptibility to an autoimmune thyroid disease occurs both within the 3′ UTR of the ZFAT gene (zinc-finger gene in AITD susceptibility region) and also within the promoter of an antisense transcript (SAS-ZFAT), and increases the expression of ZFAT not through increasing mRNA stability, but by repressing the expression of the antisense transcript .
Regulation of Complex Genetic Processes by ncRNAs
Apart from the general presumption that most ncRNAs will be involved in regulation, variations in which will often have, individually, subtle effects on phenotype, there is, in fact, general evidence of their positive genetic signatures, as ncRNAs underpin most, if not all, complex genetic processes in the higher organisms. These include RNA interference-related phenomena such as co-suppression and transcriptional gene silencing , –, as well as position effect variegation ,, hybrid dysgenesis , parental imprinting, X-chromosome dosage compensation and allelic exclusion , germ cell reprogramming , and paramutation ,, all of which involve epigenetic processes. Indeed, as noted already, there is increasing evidence that a major function of ncRNAs, both small and large, is the regulation of epigenetic memory through modifications to DNA and chromatin structure, involving the recruitment of DNA methyltransferases, histone-modifying enzymes, and chromatin remodelling complexes to their appropriate sites of action (including ncRNA genes themselves) in particular cells at particular stages of differentiation (for reviews, see ,,; also Table 1).
Examples of Mutations in ncRNAs
There are some known examples of mutations in ncRNAs, aside from those mentioned already, that give recognizable phenotypes or that are strongly implicated in altered phenotypic states. These include a triplet repeat expansion in the ncRNA SCA8, which causes the human neurodegenerative disease Spinocerebellar Ataxia 8 (which as a transgene can induce progressive retinal neurodegeneration in Drosophila)  and other examples of deleterious gain-of-function mutations in noncoding RNAs associated with diseases such as myotonic dystrophy , deletions encompassing ncRNA loci and alterations to ncRNA splicing patterns in various cancers ,,,,,, and a SNP variant in an ncRNA MIAT that confers risk of myocardial infarction . They also include many ncRNAs, including small nucleolar RNAs, that appear to be important in the mechanism of imprinting  and the molecular etiology of associated pathologies such as Prader-Willi syndrome ,, some that are implicated as tumour suppressors ,, or that are located at chromosomal translocation breakpoints associated with B-cell lymphoma  and schizophrenia . It has also been shown that the translocation and induced expression of an antisense, long ncRNA can cause the epigenetic silencing of the adjacent α-globin gene, resulting in α-thalassemia .
There is not (yet) a huge catalogue of mutations in ncRNAs that have been shown to affect phenotype, compared to those in protein-coding sequences. However, on the assumption that most ncRNAs are regulatory and that most regulatory regions have yet to be assigned genetic signatures, it is no surprise that this may be the case. On the other hand, as screening for variations affecting complex traits becomes more sophisticated, it is reasonable to anticipate that many will map to, and affect the function of, ncRNAs. Certainly this possibility should be borne in mind in the interpretation of such variations and the consequent studies to define their mechanism of action. The functional analysis of ncRNAs is in its infancy, but in situ hybridization, genomic, and structural characteristics, and the perturbation of their expression by overexpression and siRNA-mediated knockdown are emerging as major tools (Tables 1 and 2). There seems little doubt that there is a hidden world of regulatory architecture underpinning the development of complex organisms that we have yet to explore, both genetically and functionally.
I thank Paulo Amaral, Grant Montgomery, and Manolis Dermitzakis for helpful comments on the manuscript; Paulo Amaral for assistance in the construction of Table 1; Ryan Taft for providing the analysis shown in Figure 1; and Tim Mercer for drawing the figures.
- 1. Taft RJ, Pheasant M, Mattick JS (2007) The relationship between non-protein-coding DNA and eukaryotic complexity. Bioessays 29: 288–299.RJ TaftM. PheasantJS Mattick2007The relationship between non-protein-coding DNA and eukaryotic complexity.Bioessays29288299
- 2. Carninci P, Kasukawa T, Katayama S, Gough J, Frith MC, et al. (2005) The transcriptional landscape of the mammalian genome. Science 309: 1559–1563.P. CarninciT. KasukawaS. KatayamaJ. GoughMC Frith2005The transcriptional landscape of the mammalian genome.Science30915591563
- 3. Kapranov P, Drenkow J, Cheng J, Long J, Helt G, et al. (2005) Examples of the complex architecture of the human transcriptome revealed by RACE and high-density tiling arrays. Genome Res 15: 987–997.P. KapranovJ. DrenkowJ. ChengJ. LongG. Helt2005Examples of the complex architecture of the human transcriptome revealed by RACE and high-density tiling arrays.Genome Res15987997
- 4. Mattick JS, Makunin IV (2006) Non-coding RNA. Hum Mol Genet 15: R17–29.JS MattickIV Makunin2006Non-coding RNA.Hum Mol Genet15R1729
- 5. Kapranov P, Willingham AT, Gingeras TR (2007) Genome-wide transcription and the implications for genomic organization. Nat Rev Genet 8: 413–423.P. KapranovAT WillinghamTR Gingeras2007Genome-wide transcription and the implications for genomic organization.Nat Rev Genet8413423
- 6. Rajendra TK, Prasanth KV, Lakhotia SC (2001) Male sterility associated with overexpression of the noncoding hsromega gene in cyst cells of testis of Drosophila melanogaster. J Genet 80: 97–110.TK RajendraKV PrasanthSC Lakhotia2001Male sterility associated with overexpression of the noncoding hsromega gene in cyst cells of testis of Drosophila melanogaster.J Genet8097110
- 7. Sanchez-Elsner T, Gou D, Kremmer E, Sauer F (2006) Noncoding RNAs of trithorax response elements recruit Drosophila Ash1 to Ultrabithorax. Science 311: 1118–1123.T. Sanchez-ElsnerD. GouE. KremmerF. Sauer2006Noncoding RNAs of trithorax response elements recruit Drosophila Ash1 to Ultrabithorax.Science31111181123
- 8. Hellwig S, Bass BL (2008) A starvation-induced noncoding RNA modulates expression of Dicer-regulated genes. Proc Natl Acad Sci U S A 105: 12897–12902.S. HellwigBL Bass2008A starvation-induced noncoding RNA modulates expression of Dicer-regulated genes.Proc Natl Acad Sci U S A1051289712902
- 9. Ma J, Yan B, Qu Y, Qin F, Yang Y, et al. (2008) Zm401, a short-open reading-frame mRNA or noncoding RNA, is essential for tapetum and microspore development and can regulate the floret formation in maize. J Cell Biochem 105: 136–146.J. MaB. YanY. QuF. QinY. Yang2008Zm401, a short-open reading-frame mRNA or noncoding RNA, is essential for tapetum and microspore development and can regulate the floret formation in maize.J Cell Biochem105136146
- 10. Ben Amor B, Wirth S, Merchan F, Laporte P, d'Aubenton-Carafa Y, et al. (2009) Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses. Genome Res 19: 57–69.B. Ben AmorS. WirthF. MerchanP. LaporteY. d'Aubenton-Carafa2009Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses.Genome Res195769
- 11. Camblong J, Iglesias N, Fickentscher C, Dieppois G, Stutz F (2007) Antisense RNA stabilization induces transcriptional gene silencing via histone deacetylation in S. cerevisiae. Cell 131: 706–717.J. CamblongN. IglesiasC. FickentscherG. DieppoisF. Stutz2007Antisense RNA stabilization induces transcriptional gene silencing via histone deacetylation in S. cerevisiae.Cell131706717
- 12. Amaral PP, Mattick JS (2008) Noncoding RNA in development. Mamm Genome 19: 454–492.PP AmaralJS Mattick2008Noncoding RNA in development.Mamm Genome19454492
- 13. Rinn JL, Kertesz M, Wang JK, Squazzo SL, Xu X, et al. (2007) Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 129: 1311–1323.JL RinnM. KerteszJK WangSL SquazzoX. Xu2007Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs.Cell12913111323
- 14. Yu W, Gius D, Onyango P, Muldoon-Jacobs K, Karp J, et al. (2008) Epigenetic silencing of tumour suppressor gene p15 by its antisense RNA. Nature 451: 202–206.W. YuD. GiusP. OnyangoK. Muldoon-JacobsJ. Karp2008Epigenetic silencing of tumour suppressor gene p15 by its antisense RNA.Nature451202206
- 15. Martianov I, Ramadass A, Serra Barros A, Chow N, Akoulitchev A (2007) Repression of the human dihydrofolate reductase gene by a non-coding interfering transcript. Nature 445: 666–670.I. MartianovA. RamadassA. Serra BarrosN. ChowA. Akoulitchev2007Repression of the human dihydrofolate reductase gene by a non-coding interfering transcript.Nature445666670
- 16. Blin-Wakkach C, Lezot F, Ghoul-Mazgar S, Hotton D, Monteiro S, et al. (2001) Endogenous Msx1 antisense transcript: in vivo and in vitro evidences, structure, and potential involvement in skeleton development in mammals. Proc Natl Acad Sci U S A 98: 7336–7341.C. Blin-WakkachF. LezotS. Ghoul-MazgarD. HottonS. Monteiro2001Endogenous Msx1 antisense transcript: in vivo and in vitro evidences, structure, and potential involvement in skeleton development in mammals.Proc Natl Acad Sci U S A9873367341
- 17. Young TL, Matsuda T, Cepko CL (2005) The noncoding RNA taurine upregulated gene 1 is required for differentiation of the murine retina. Curr Biol 15: 501–512.TL YoungT. MatsudaCL Cepko2005The noncoding RNA taurine upregulated gene 1 is required for differentiation of the murine retina.Curr Biol15501512
- 18. Cano A, Nieto MA (2008) Non-coding RNAs take centre stage in epithelial-to-mesenchymal transition. Trends Cell Biol 18: 357–359.A. CanoMA Nieto2008Non-coding RNAs take centre stage in epithelial-to-mesenchymal transition.Trends Cell Biol18357359
- 19. Sunwoo H, Dinger ME, Wilusz JE, Amaral PP, Mattick JS, et al. (2009) MEN ε/β nuclear-retained non-coding RNAs are up-regulated upon muscle differentiation and are essential components of paraspeckles. Genome Res 19: 347–359.H. SunwooME DingerJE WiluszPP AmaralJS Mattick2009MEN ε/β nuclear-retained non-coding RNAs are up-regulated upon muscle differentiation and are essential components of paraspeckles.Genome Res19347359
- 20. Sasaki YT, Ideue T, Sano M, Mituyama T, Hirose T (2009) MENepsilon/beta noncoding RNAs are essential for structural integrity of nuclear paraspeckles. Proc Natl Acad Sci U S A 106: 2525–2530.YT SasakiT. IdeueM. SanoT. MituyamaT. Hirose2009MENepsilon/beta noncoding RNAs are essential for structural integrity of nuclear paraspeckles.Proc Natl Acad Sci U S A10625252530
- 21. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, et al. (2009) An architectural role for a nuclear noncoding RNA: NEAT1 RNA Is essential for the structure of paraspeckles. Mol Cell. CM ClemsonJN HutchinsonSA SaraAW EnsmingerAH Fox2009An architectural role for a nuclear noncoding RNA: NEAT1 RNA Is essential for the structure of paraspeckles.Mol Cellepub ahead of print.
- 22. Redrup L, Branco MR, Perdeaux ER, Krueger C, Lewis A, et al. (2009) The long noncoding RNA Kcnq1ot1 organises a lineage-specific nuclear domain for epigenetic gene silencing. Development 136: 525–530.L. RedrupMR BrancoER PerdeauxC. KruegerA. Lewis2009The long noncoding RNA Kcnq1ot1 organises a lineage-specific nuclear domain for epigenetic gene silencing.Development136525530
- 23. Prasanth KV, Spector DL (2007) Eukaryotic regulatory RNAs: an answer to the ‘genome complexity’ conundrum. Genes Dev 21: 11–42.KV PrasanthDL Spector2007Eukaryotic regulatory RNAs: an answer to the ‘genome complexity’ conundrum.Genes Dev211142
- 24. Louro R, Smirnova AS, Verjovski-Almeida S (2009) Long intronic noncoding RNA transcription: expression noise or expression choice? Genomics 93: 291–298.R. LouroAS SmirnovaS. Verjovski-Almeida2009Long intronic noncoding RNA transcription: expression noise or expression choice?Genomics93291298
- 25. Mercer TR, Dinger ME, Mattick JS (2009) Long non-coding RNAs: insights into functions. Nat Rev Genet 10: 155–159.TR MercerME DingerJS Mattick2009Long non-coding RNAs: insights into functions.Nat Rev Genet10155159
- 26. Pang KC, Frith MC, Mattick JS (2006) Rapid evolution of noncoding RNAs: lack of conservation does not mean lack of function. Trends Genet 22: 1–5.KC PangMC FrithJS Mattick2006Rapid evolution of noncoding RNAs: lack of conservation does not mean lack of function.Trends Genet2215
- 27. Ponjavic J, Ponting CP, Lunter G (2007) Functionality or transcriptional noise? Evidence for selection within long noncoding RNAs. Genome Res 17: 556–565.J. PonjavicCP PontingG. Lunter2007Functionality or transcriptional noise? Evidence for selection within long noncoding RNAs.Genome Res17556565
- 28. Washietl S, Hofacker IL, Lukasser M, Huttenhofer A, Stadler PF (2005) Mapping of conserved RNA secondary structures predicts thousands of functional noncoding RNAs in the human genome. Nat Biotechnol 23: 1383–1390.S. WashietlIL HofackerM. LukasserA. HuttenhoferPF Stadler2005Mapping of conserved RNA secondary structures predicts thousands of functional noncoding RNAs in the human genome.Nat Biotechnol2313831390
- 29. Torarinsson E, Sawera M, Havgaard JH, Fredholm M, Gorodkin J (2006) Thousands of corresponding human and mouse genomic regions unalignable in primary sequence contain common RNA structure. Genome Res 16: 885–889.E. TorarinssonM. SaweraJH HavgaardM. FredholmJ. Gorodkin2006Thousands of corresponding human and mouse genomic regions unalignable in primary sequence contain common RNA structure.Genome Res16885889
- 30. Torarinsson E, Yao Z, Wiklund ED, Bramsen JB, Hansen C, et al. (2008) Comparative genomics beyond sequence-based alignments: RNA structures in the ENCODE regions. Genome Res 18: 242–251.E. TorarinssonZ. YaoED WiklundJB BramsenC. Hansen2008Comparative genomics beyond sequence-based alignments: RNA structures in the ENCODE regions.Genome Res18242251
- 31. Dinger ME, Amaral PP, Mercer TR, Pang KC, Bruce SJ, et al. (2008) Long noncoding RNAs in mouse embryonic stem cell pluripotency and differentiation. Genome Res 18: 1433–1445.ME DingerPP AmaralTR MercerKC PangSJ Bruce2008Long noncoding RNAs in mouse embryonic stem cell pluripotency and differentiation.Genome Res1814331445
- 32. Guttman M, Amit I, Garber M, French C, Lin MF, et al. (2009) Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature 458: 223–227.M. GuttmanI. AmitM. GarberC. FrenchMF Lin2009Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals.Nature458223227
- 33. Trinklein ND, Aldred SF, Hartman SJ, Schroeder DI, Otillar RP, et al. (2004) An abundance of bidirectional promoters in the human genome. Genome Res 14: 62–66.ND TrinkleinSF AldredSJ HartmanDI SchroederRP Otillar2004An abundance of bidirectional promoters in the human genome.Genome Res146266
- 34. Engstrom PG, Suzuki H, Ninomiya N, Akalin A, Sessa L, et al. (2006) Complex loci in human and mouse genomes. PLoS Genet 2: e47.PG EngstromH. SuzukiN. NinomiyaA. AkalinL. Sessa2006Complex loci in human and mouse genomes.PLoS Genet2e47
- 35. Tupy JL, Bailey AM, Dailey G, Evans-Holm M, Siebel CW, et al. (2005) Identification of putative noncoding polyadenylated transcripts in Drosophila melanogaster. Proc Natl Acad Sci U S A 102: 5495–5500.JL TupyAM BaileyG. DaileyM. Evans-HolmCW Siebel2005Identification of putative noncoding polyadenylated transcripts in Drosophila melanogaster.Proc Natl Acad Sci U S A10254955500
- 36. Louro R, El-Jundi T, Nakaya HI, Reis EM, Verjovski-Almeida S (2008) Conserved tissue expression signatures of intronic noncoding RNAs transcribed from human and mouse loci. Genomics 92: 18–25.R. LouroT. El-JundiHI NakayaEM ReisS. Verjovski-Almeida2008Conserved tissue expression signatures of intronic noncoding RNAs transcribed from human and mouse loci.Genomics921825
- 37. Thrash-Bingham CA, Tartof KD (1999) aHIF: a natural antisense transcript overexpressed in human renal cancer and during hypoxia. J Natl Cancer Inst 91: 143–151.CA Thrash-BinghamKD Tartof1999aHIF: a natural antisense transcript overexpressed in human renal cancer and during hypoxia.J Natl Cancer Inst91143151
- 38. Ji P, Diederichs S, Wang W, Boing S, Metzger R, et al. (2003) MALAT-1, a novel noncoding RNA, and thymosin beta4 predict metastasis and survival in early-stage non-small cell lung cancer. Oncogene 22: 6087–6097.P. JiS. DiederichsW. WangS. BoingR. Metzger2003MALAT-1, a novel noncoding RNA, and thymosin beta4 predict metastasis and survival in early-stage non-small cell lung cancer.Oncogene2260876097
- 39. Mutsuddi M, Marshall CM, Benzow KA, Koob MD, Rebay I (2004) The spinocerebellar ataxia 8 noncoding RNA causes neurodegeneration and associates with staufen in Drosophila. Curr Biol 14: 302–308.M. MutsuddiCM MarshallKA BenzowMD KoobI. Rebay2004The spinocerebellar ataxia 8 noncoding RNA causes neurodegeneration and associates with staufen in Drosophila.Curr Biol14302308
- 40. Reis EM, Nakaya HI, Louro R, Canavez FC, Flatschart AV, et al. (2004) Antisense intronic non-coding RNA levels correlate to the degree of tumor differentiation in prostate cancer. Oncogene 23: 6684–6692.EM ReisHI NakayaR. LouroFC CanavezAV Flatschart2004Antisense intronic non-coding RNA levels correlate to the degree of tumor differentiation in prostate cancer.Oncogene2366846692
- 41. Reis EM, Ojopi EP, Alberto FL, Rahal P, Tsukumo F, et al. (2005) Large-scale transcriptome analyses reveal new genetic marker candidates of head, neck, and thyroid cancer. Cancer Res 65: 1693–1699.EM ReisEP OjopiFL AlbertoP. RahalF. Tsukumo2005Large-scale transcriptome analyses reveal new genetic marker candidates of head, neck, and thyroid cancer.Cancer Res6516931699
- 42. Sonkoly E, Bata-Csorgo Z, Pivarcsi A, Polyanka H, Kenderessy-Szabo A, et al. (2005) Identification and characterization of a novel, psoriasis susceptibility-related noncoding RNA gene, PRINS. J Biol Chem 280: 24159–24167.E. SonkolyZ. Bata-CsorgoA. PivarcsiH. PolyankaA. Kenderessy-Szabo2005Identification and characterization of a novel, psoriasis susceptibility-related noncoding RNA gene, PRINS.J Biol Chem2802415924167
- 43. Szymanski M, Barciszewska MZ, Erdmann VA, Barciszewski J (2005) A new frontier for molecular medicine: noncoding RNAs. Biochim Biophys Acta 1756: 65–75.M. SzymanskiMZ BarciszewskaVA ErdmannJ. Barciszewski2005A new frontier for molecular medicine: noncoding RNAs.Biochim Biophys Acta17566575
- 44. Angeloni D, ter Elst A, Wei MH, van der Veen AY, Braga EA, et al. (2006) Analysis of a new homozygous deletion in the tumor suppressor region at 3p12.3 reveals two novel intronic noncoding RNA genes. Genes Chromosomes Cancer 45: 676–691.D. AngeloniA. ter ElstMH WeiAY van der VeenEA Braga2006Analysis of a new homozygous deletion in the tumor suppressor region at 3p12.3 reveals two novel intronic noncoding RNA genes.Genes Chromosomes Cancer45676691
- 45. Calin GA, Liu CG, Ferracin M, Hyslop T, Spizzo R, et al. (2007) Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas. Cancer Cell 12: 215–229.GA CalinCG LiuM. FerracinT. HyslopR. Spizzo2007Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas.Cancer Cell12215229
- 46. Pang KC, Stephen S, Dinger ME, Engstrom PG, Lenhard B, et al. (2007) RNAdb 2.0–an expanded database of mammalian non-coding RNAs. Nucleic Acids Res 35: D178–182.KC PangS. StephenME DingerPG EngstromB. Lenhard2007RNAdb 2.0–an expanded database of mammalian non-coding RNAs.Nucleic Acids Res35D178182
- 47. Christov CP, Trivier E, Krude T (2008) Noncoding human Y RNAs are overexpressed in tumours and required for cell proliferation. Br J Cancer 98: 981–988.CP ChristovE. TrivierT. Krude2008Noncoding human Y RNAs are overexpressed in tumours and required for cell proliferation.Br J Cancer98981988
- 48. Perez DS, Hoage TR, Pritchett JR, Ducharme-Smith AL, Halling ML, et al. (2008) Long, abundantly expressed non-coding transcripts are altered in cancer. Hum Mol Genet 17: 642–655.DS PerezTR HoageJR PritchettAL Ducharme-SmithML Halling2008Long, abundantly expressed non-coding transcripts are altered in cancer.Hum Mol Genet17642655
- 49. Zhang X, Lian Z, Padden C, Gerstein MB, Rozowsky J, et al. (2009) A myelopoiesis-associated regulatory intergenic non-coding RNA transcript within the human HOXA cluster. Blood 113: 2526–2534.X. ZhangZ. LianC. PaddenMB GersteinJ. Rozowsky2009A myelopoiesis-associated regulatory intergenic non-coding RNA transcript within the human HOXA cluster.Blood11325262534
- 50. Cawley S, Bekiranov S, Ng HH, Kapranov P, Sekinger EA, et al. (2004) Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs. Cell 116: 499–509.S. CawleyS. BekiranovHH NgP. KapranovEA Sekinger2004Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs.Cell116499509
- 51. Ravasi T, Suzuki H, Pang KC, Katayama S, Furuno M, et al. (2006) Experimental validation of the regulated expression of large numbers of non-coding RNAs from the mouse genome. Genome Res 16: 11–19.T. RavasiH. SuzukiKC PangS. KatayamaM. Furuno2006Experimental validation of the regulated expression of large numbers of non-coding RNAs from the mouse genome.Genome Res161119
- 52. Mercer TR, Dinger ME, Sunkin SM, Mehler MF, Mattick JS (2008) Specific expression of long noncoding RNAs in the mouse brain. Proc Natl Acad Sci U S A 105: 716–721.TR MercerME DingerSM SunkinMF MehlerJS Mattick2008Specific expression of long noncoding RNAs in the mouse brain.Proc Natl Acad Sci U S A105716721
- 53. Kohtz JD, Fishell G (2004) Developmental regulation of EVF-1, a novel non-coding RNA transcribed upstream of the mouse Dlx6 gene. Gene Expr Patterns 4: 407–412.JD KohtzG. Fishell2004Developmental regulation of EVF-1, a novel non-coding RNA transcribed upstream of the mouse Dlx6 gene.Gene Expr Patterns4407412
- 54. Inagaki S, Numata K, Kondo T, Tomita M, Yasuda K, et al. (2005) Identification and expression analysis of putative mRNA-like non-coding RNA in Drosophila. Genes Cells 10: 1163–1173.S. InagakiK. NumataT. KondoM. TomitaK. Yasuda2005Identification and expression analysis of putative mRNA-like non-coding RNA in Drosophila.Genes Cells1011631173
- 55. Brena C, Chipman AD, Minelli A, Akam M (2006) Expression of trunk Hox genes in the centipede Strigamia maritima: sense and anti-sense transcripts. Evol Dev 8: 252–265.C. BrenaAD ChipmanA. MinelliM. Akam2006Expression of trunk Hox genes in the centipede Strigamia maritima: sense and anti-sense transcripts.Evol Dev8252265
- 56. Sone M, Hayashi T, Tarui H, Agata K, Takeichi M, et al. (2007) The mRNA-like noncoding RNA Gomafu constitutes a novel nuclear domain in a subset of neurons. J Cell Sci 120: 2498–2506.M. SoneT. HayashiH. TaruiK. AgataM. Takeichi2007The mRNA-like noncoding RNA Gomafu constitutes a novel nuclear domain in a subset of neurons.J Cell Sci12024982506
- 57. Pollard KS, Salama SR, King B, Kern AD, Dreszer T, et al. (2006) Forces shaping the fastest evolving regions in the human genome. PLoS Genet 2: e168.KS PollardSR SalamaB. KingAD KernT. Dreszer2006Forces shaping the fastest evolving regions in the human genome.PLoS Genet2e168
- 58. Rodriguez A, Griffiths-Jones S, Ashurst JL, Bradley A (2004) Identification of mammalian microRNA host genes and transcription units. Genome Res 14: 1902–1910.A. RodriguezS. Griffiths-JonesJL AshurstA. Bradley2004Identification of mammalian microRNA host genes and transcription units.Genome Res1419021910
- 59. Aravin AA, Hannon GJ, Brennecke J (2007) The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race. Science 318: 761–764.AA AravinGJ HannonJ. Brennecke2007The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race.Science318761764
- 60. Ruby JG, Jan CH, Bartel DP (2007) Intronic microRNA precursors that bypass Drosha processing. Nature 448: 83–86.JG RubyCH JanDP Bartel2007Intronic microRNA precursors that bypass Drosha processing.Nature4488386
- 61. Okamura K, Hagen JW, Duan H, Tyler DM, Lai EC (2007) The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. Cell 130: 89–100.K. OkamuraJW HagenH. DuanDM TylerEC Lai2007The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila.Cell13089100
- 62. Berezikov E, Chung WJ, Willis J, Cuppen E, Lai EC (2007) Mammalian mirtron genes. Mol Cell 28: 328–336.E. BerezikovWJ ChungJ. WillisE. CuppenEC Lai2007Mammalian mirtron genes.Mol Cell28328336
- 63. Tam OH, Aravin AA, Stein P, Girard A, Murchison EP, et al. (2008) Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes. Nature 453: 534–538.OH TamAA AravinP. SteinA. GirardEP Murchison2008Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes.Nature453534538
- 64. Watanabe T, Totoki Y, Toyoda A, Kaneda M, Kuramochi-Miyagawa S, et al. (2008) Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes. Nature 453: 539–543.T. WatanabeY. TotokiA. ToyodaM. KanedaS. Kuramochi-Miyagawa2008Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes.Nature453539543
- 65. Okamura K, Chung WJ, Ruby JG, Guo H, Bartel DP, et al. (2008) The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs. Nature 453: 803–806.K. OkamuraWJ ChungJG RubyH. GuoDP Bartel2008The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs.Nature453803806
- 66. Klattenhoff C, Theurkauf W (2008) Biogenesis and germline functions of piRNAs. Development 135: 3–9.C. KlattenhoffW. Theurkauf2008Biogenesis and germline functions of piRNAs.Development13539
- 67. Stefani G, Slack FJ (2008) Small non-coding RNAs in animal development. Nat Rev Mol Cell Biol 9: 219–230.G. StefaniFJ Slack2008Small non-coding RNAs in animal development.Nat Rev Mol Cell Biol9219230
- 68. Ogawa Y, Sun BK, Lee JT (2008) Intersection of the RNA interference and X-inactivation pathways. Science 320: 1336–1341.Y. OgawaBK SunJT Lee2008Intersection of the RNA interference and X-inactivation pathways.Science32013361341
- 69. Bentwich I, Avniel A, Karov Y, Aharonov R, Gilad S, et al. (2005) Identification of hundreds of conserved and nonconserved human microRNAs. Nat Genet 37: 766–770.I. BentwichA. AvnielY. KarovR. AharonovS. Gilad2005Identification of hundreds of conserved and nonconserved human microRNAs.Nat Genet37766770
- 70. Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, et al. (2006) Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis. Genome Res 16: 1289–1298.E. BerezikovG. van TeteringM. VerheulJ. van de BeltL. van Laake2006Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis.Genome Res1612891298
- 71. Carroll SB (2008) Evo-devo and an expanding evolutionary synthesis: a genetic theory of morphological evolution. Cell 134: 25–36.SB Carroll2008Evo-devo and an expanding evolutionary synthesis: a genetic theory of morphological evolution.Cell1342536
- 72. Smith NG, Brandstrom M, Ellegren H (2004) Evidence for turnover of functional noncoding DNA in mammalian genome evolution. Genomics 84: 806–813.NG SmithM. BrandstromH. Ellegren2004Evidence for turnover of functional noncoding DNA in mammalian genome evolution.Genomics84806813
- 73. Frith MC, Ponjavic J, Fredman D, Kai C, Kawai J, et al. (2006) Evolutionary turnover of mammalian transcription start sites. Genome Res 16: 713–722.MC FrithJ. PonjavicD. FredmanC. KaiJ. Kawai2006Evolutionary turnover of mammalian transcription start sites.Genome Res16713722
- 74. Taylor MS, Kai C, Kawai J, Carninci P, Hayashizaki Y, et al. (2006) Heterotachy in mammalian promoter evolution. PLoS Genet 2: e30.MS TaylorC. KaiJ. KawaiP. CarninciY. Hayashizaki2006Heterotachy in mammalian promoter evolution.PLoS Genet2e30
- 75. Pheasant M, Mattick JS (2007) Raising the estimate of functional human sequences. Genome Res 17: 1245–1253.M. PheasantJS Mattick2007Raising the estimate of functional human sequences.Genome Res1712451253
- 76. Fisher S, Grice EA, Vinton RM, Bessling SL, McCallion AS (2006) Conservation of RET regulatory function from human to zebrafish without sequence similarity. Science 312: 276–279.S. FisherEA GriceRM VintonSL BesslingAS McCallion2006Conservation of RET regulatory function from human to zebrafish without sequence similarity.Science312276279
- 77. Dermitzakis ET, Reymond A, Lyle R, Scamuffa N, Ucla C, et al. (2002) Numerous potentially functional but non-genic conserved sequences on human chromosome 21. Nature 420: 578–582.ET DermitzakisA. ReymondR. LyleN. ScamuffaC. Ucla2002Numerous potentially functional but non-genic conserved sequences on human chromosome 21.Nature420578582
- 78. Dermitzakis ET, Reymond A, Scamuffa N, Ucla C, Kirkness E, et al. (2003) Evolutionary discrimination of mammalian conserved non-genic sequences (CNGs). Science 302: 1033–1035.ET DermitzakisA. ReymondN. ScamuffaC. UclaE. Kirkness2003Evolutionary discrimination of mammalian conserved non-genic sequences (CNGs).Science30210331035
- 79. Bejerano G, Pheasant M, Makunin I, Stephen S, Kent WJ, et al. (2004) Ultraconserved elements in the human genome. Science 304: 1321–1325.G. BejeranoM. PheasantI. MakuninS. StephenWJ Kent2004Ultraconserved elements in the human genome.Science30413211325
- 80. Stephen S, Pheasant M, Makunin IV, Mattick JS (2008) Large-scale appearance of ultraconserved elements in tetrapod genomes and slowdown of the molecular clock. Mol Biol Evol 25: 402–408.S. StephenM. PheasantIV MakuninJS Mattick2008Large-scale appearance of ultraconserved elements in tetrapod genomes and slowdown of the molecular clock.Mol Biol Evol25402408
- 81. Pennacchio LA, Ahituv N, Moses AM, Prabhakar S, Nobrega MA, et al. (2006) In vivo enhancer analysis of human conserved non-coding sequences. Nature 444: 499–502.LA PennacchioN. AhituvAM MosesS. PrabhakarMA Nobrega2006In vivo enhancer analysis of human conserved non-coding sequences.Nature444499502
- 82. Visel A, Prabhakar S, Akiyama JA, Shoukry M, Lewis KD, et al. (2008) Ultraconservation identifies a small subset of extremely constrained developmental enhancers. Nat Genet 40: 158–160.A. ViselS. PrabhakarJA AkiyamaM. ShoukryKD Lewis2008Ultraconservation identifies a small subset of extremely constrained developmental enhancers.Nat Genet40158160
- 83. Ashe HL, Monks J, Wijgerde M, Fraser P, Proudfoot NJ (1997) Intergenic transcription and transinduction of the human beta-globin locus. Genes Dev 11: 2494–2509.HL AsheJ. MonksM. WijgerdeP. FraserNJ Proudfoot1997Intergenic transcription and transinduction of the human beta-globin locus.Genes Dev1124942509
- 84. Ling J, Baibakov B, Pi W, Emerson BM, Tuan D (2005) The HS2 enhancer of the beta-globin locus control region initiates synthesis of non-coding, polyadenylated RNAs independent of a cis-linked globin promoter. J Mol Biol 350: 883–896.J. LingB. BaibakovW. PiBM EmersonD. Tuan2005The HS2 enhancer of the beta-globin locus control region initiates synthesis of non-coding, polyadenylated RNAs independent of a cis-linked globin promoter.J Mol Biol350883896
- 85. Lefevre P, Witham J, Lacroix CE, Cockerill PN, Bonifer C (2008) The LPS-induced transcriptional upregulation of the chicken lysozyme locus involves CTCF eviction and noncoding RNA transcription. Mol Cell 32: 129–139.P. LefevreJ. WithamCE LacroixPN CockerillC. Bonifer2008The LPS-induced transcriptional upregulation of the chicken lysozyme locus involves CTCF eviction and noncoding RNA transcription.Mol Cell32129139
- 86. Lempradl A, Ringrose L (2008) How does noncoding transcription regulate Hox genes? Bioessays 30: 110–121.A. LempradlL. Ringrose2008How does noncoding transcription regulate Hox genes?Bioessays30110121
- 87. Mattick JS (2003) Challenging the dogma: the hidden layer of non-protein-coding RNAs in complex organisms. Bioessays 25: 930–939.JS Mattick2003Challenging the dogma: the hidden layer of non-protein-coding RNAs in complex organisms.Bioessays25930939
- 88. Mattick JS (2007) A new paradigm for developmental biology. J Exp Biol 210: 1526–1547.JS Mattick2007A new paradigm for developmental biology.J Exp Biol21015261547
- 89. Mattick JS, Amaral PP, Dinger ME, Mercer TR, Mehler MF (2009) RNA regulation of epigenetic processes. Bioessays 31: 51–59.JS MattickPP AmaralME DingerTR MercerMF Mehler2009RNA regulation of epigenetic processes.Bioessays315159
- 90. Liu ET (2008) Functional genomics of cancer. Curr Opin Genet Dev 18: 251–256.ET Liu2008Functional genomics of cancer.Curr Opin Genet Dev18251256
- 91. Tomasini R, Mak TW, Melino G (2008) The impact of p53 and p73 on aneuploidy and cancer. Trends Cell Biol 18: 244–252.R. TomasiniTW MakG. Melino2008The impact of p53 and p73 on aneuploidy and cancer.Trends Cell Biol18244252
- 92. Barbaric I, Miller G, Dear TN (2007) Appearances can be deceiving: phenotypes of knockout mice. Brief Funct Genomic Proteomic 6: 91–103.I. BarbaricG. MillerTN Dear2007Appearances can be deceiving: phenotypes of knockout mice.Brief Funct Genomic Proteomic691103
- 93. Kutuzova GD, Akhter S, Christakos S, Vanhooke J, Kimmel-Jehan C, et al. (2006) Calbindin D(9k) knockout mice are indistinguishable from wild-type mice in phenotype and serum calcium level. Proc Natl Acad Sci U S A 103: 12377–12381.GD KutuzovaS. AkhterS. ChristakosJ. VanhookeC. Kimmel-Jehan2006Calbindin D(9k) knockout mice are indistinguishable from wild-type mice in phenotype and serum calcium level.Proc Natl Acad Sci U S A1031237712381
- 94. Crawley JN (1999) Behavioral phenotyping of transgenic and knockout mice: experimental design and evaluation of general health, sensory functions, motor abilities, and specific behavioral tests. Brain Res 835: 18–26.JN Crawley1999Behavioral phenotyping of transgenic and knockout mice: experimental design and evaluation of general health, sensory functions, motor abilities, and specific behavioral tests.Brain Res8351826
- 95. Zhao Y, Ransom JF, Li A, Vedantham V, von Drehle M, et al. (2007) Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2. Cell 129: 303–317.Y. ZhaoJF RansomA. LiV. VedanthamM. von Drehle2007Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2.Cell129303317
- 96. Thai TH, Calado DP, Casola S, Ansel KM, Xiao C, et al. (2007) Regulation of the germinal center response by microRNA-155. Science 316: 604–608.TH ThaiDP CaladoS. CasolaKM AnselC. Xiao2007Regulation of the germinal center response by microRNA-155.Science316604608
- 97. Rodriguez A, Vigorito E, Clare S, Warren MV, Couttet P, et al. (2007) Requirement of bic/microRNA-155 for normal immune function. Science 316: 608–611.A. RodriguezE. VigoritoS. ClareMV WarrenP. Couttet2007Requirement of bic/microRNA-155 for normal immune function.Science316608611
- 98. van Rooij E, Sutherland LB, Qi X, Richardson JA, Hill J, et al. (2007) Control of stress-dependent cardiac growth and gene expression by a microRNA. Science 316: 575–579.E. van RooijLB SutherlandX. QiJA RichardsonJ. Hill2007Control of stress-dependent cardiac growth and gene expression by a microRNA.Science316575579
- 99. Ng K, Pullirsch D, Leeb M, Wutz A (2007) Xist and the order of silencing. EMBO Rep 8: 34–39.K. NgD. PullirschM. LeebA. Wutz2007Xist and the order of silencing.EMBO Rep83439
- 100. Lewejohann L, Skryabin BV, Sachser N, Prehn C, Heiduschka P, et al. (2004) Role of a neuronal small non-messenger RNA: behavioural alterations in BC1 RNA-deleted mice. Behav Brain Res 154: 273–289.L. LewejohannBV SkryabinN. SachserC. PrehnP. Heiduschka2004Role of a neuronal small non-messenger RNA: behavioural alterations in BC1 RNA-deleted mice.Behav Brain Res154273289
- 101. Poulin F, Nobrega MA, Plajzer-Frick I, Holt A, Afzal V, et al. (2005) In vivo characterization of a vertebrate ultraconserved enhancer. Genomics 85: 774–781.F. PoulinMA NobregaI. Plajzer-FrickA. HoltV. Afzal2005In vivo characterization of a vertebrate ultraconserved enhancer.Genomics85774781
- 102. Ahituv N, Zhu Y, Visel A, Holt A, Afzal V, et al. (2007) Deletion of ultraconserved elements yields viable mice. PLoS Biol 5: e234.N. AhituvY. ZhuA. ViselA. HoltV. Afzal2007Deletion of ultraconserved elements yields viable mice.PLoS Biol5e234
- 103. Katzman S, Kern AD, Bejerano G, Fewell G, Fulton L, et al. (2007) Human genome ultraconserved elements are ultraselected. Science 317: 915.S. KatzmanAD KernG. BejeranoG. FewellL. Fulton2007Human genome ultraconserved elements are ultraselected.Science317915
- 104. Hindorff LA, Junkins HA, Manolio TA (2008) A catalog of published genome-wide association studies. LA HindorffHA JunkinsTA Manolio2008A catalog of published genome-wide association studies.Available at: www.genome.gov/26525384/. Available at: www.genome.gov/26525384/.
- 105. Manolio TA, Brooks LD, Collins FS (2008) A HapMap harvest of insights into the genetics of common disease. J Clin Invest 118: 1590–1605.TA ManolioLD BrooksFS Collins2008A HapMap harvest of insights into the genetics of common disease.J Clin Invest11815901605
- 106. Pasmant E, Laurendeau I, Heron D, Vidaud M, Vidaud D, et al. (2007) Characterization of a germ-line deletion, including the entire INK4/ARF locus, in a melanoma-neural system tumor family: identification of ANRIL, an antisense noncoding RNA whose expression coclusters with ARF. Cancer Res 67: 3963–3969.E. PasmantI. LaurendeauD. HeronM. VidaudD. Vidaud2007Characterization of a germ-line deletion, including the entire INK4/ARF locus, in a melanoma-neural system tumor family: identification of ANRIL, an antisense noncoding RNA whose expression coclusters with ARF.Cancer Res6739633969
- 107. Scott LJ, Mohlke KL, Bonnycastle LL, Willer CJ, Li Y, et al. (2007) A genome-wide association study of type 2 diabetes in Finns detects multiple susceptibility variants. Science 316: 1341–1345.LJ ScottKL MohlkeLL BonnycastleCJ WillerY. Li2007A genome-wide association study of type 2 diabetes in Finns detects multiple susceptibility variants.Science31613411345
- 108. Zeggini E, Weedon MN, Lindgren CM, Frayling TM, Elliott KS, et al. (2007) Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes. Science 316: 1336–1341.E. ZegginiMN WeedonCM LindgrenTM FraylingKS Elliott2007Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes.Science31613361341
- 109. McPherson R, Pertsemlidis A, Kavaslar N, Stewart A, Roberts R, et al. (2007) A common allele on chromosome 9 associated with coronary heart disease. Science 316: 1488–1491.R. McPhersonA. PertsemlidisN. KavaslarA. StewartR. Roberts2007A common allele on chromosome 9 associated with coronary heart disease.Science31614881491
- 110. Helgadottir A, Thorleifsson G, Manolescu A, Gretarsdottir S, Blondal T, et al. (2007) A common variant on chromosome 9p21 affects the risk of myocardial infarction. Science 316: 1491–1493.A. HelgadottirG. ThorleifssonA. ManolescuS. GretarsdottirT. Blondal2007A common variant on chromosome 9p21 affects the risk of myocardial infarction.Science31614911493
- 111. Broadbent HM, Peden JF, Lorkowski S, Goel A, Ongen H, et al. (2008) Susceptibility to coronary artery disease and diabetes is encoded by distinct, tightly linked SNPs in the ANRIL locus on chromosome 9p. Hum Mol Genet 17: 806–814.HM BroadbentJF PedenS. LorkowskiA. GoelH. Ongen2008Susceptibility to coronary artery disease and diabetes is encoded by distinct, tightly linked SNPs in the ANRIL locus on chromosome 9p.Hum Mol Genet17806814
- 112. Schaefer AS, Richter GM, Groessner-Schreiber B, Noack B, Nothnagel M, et al. (2009) Identification of a shared genetic susceptibility locus for coronary heart disease and periodontitis. PLoS Genet 5: e1000378.AS SchaeferGM RichterB. Groessner-SchreiberB. NoackM. Nothnagel2009Identification of a shared genetic susceptibility locus for coronary heart disease and periodontitis.PLoS Genet5e1000378
- 113. Wray NR, Goddard ME, Visscher PM (2008) Prediction of individual genetic risk of complex disease. Curr Opin Genet Dev 18: 257–263.NR WrayME GoddardPM Visscher2008Prediction of individual genetic risk of complex disease.Curr Opin Genet Dev18257263
- 114. Clark RM, Wagler TN, Quijada P, Doebley J (2006) A distant upstream enhancer at the maize domestication gene tb1 has pleiotropic effects on plant and inflorescent architecture. Nat Genet 38: 594–597.RM ClarkTN WaglerP. QuijadaJ. Doebley2006A distant upstream enhancer at the maize domestication gene tb1 has pleiotropic effects on plant and inflorescent architecture.Nat Genet38594597
- 115. Abelson JF, Kwan KY, O'Roak BJ, Baek DY, Stillman AA, et al. (2005) Sequence variants in SLITRK1 are associated with Tourette's syndrome. Science 310: 317–320.JF AbelsonKY KwanBJ O'RoakDY BaekAA Stillman2005Sequence variants in SLITRK1 are associated with Tourette's syndrome.Science310317320
- 116. Clop A, Marcq F, Takeda H, Pirottin D, Tordoir X, et al. (2006) A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nat Genet 38: 813–818.A. ClopF. MarcqH. TakedaD. PirottinX. Tordoir2006A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep.Nat Genet38813818
- 117. Van Laere AS, Nguyen M, Braunschweig M, Nezer C, Collette C, et al. (2003) A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 425: 832–836.AS Van LaereM. NguyenM. BraunschweigC. NezerC. Collette2003A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig.Nature425832836
- 118. Smit M, Segers K, Carrascosa LG, Shay T, Baraldi F, et al. (2003) Mosaicism of Solid Gold supports the causality of a noncoding A-to-G transition in the determinism of the callipyge phenotype. Genetics 163: 453–456.M. SmitK. SegersLG CarrascosaT. ShayF. Baraldi2003Mosaicism of Solid Gold supports the causality of a noncoding A-to-G transition in the determinism of the callipyge phenotype.Genetics163453456
- 119. Hagan JP, O'Neill BL, Stewart CL, Kozlov SV, Croce CM (2009) At least ten genes define the imprinted Dlk1-Dio3 cluster on mouse chromosome 12qF1. PLoS ONE 4: e4352.JP HaganBL O'NeillCL StewartSV KozlovCM Croce2009At least ten genes define the imprinted Dlk1-Dio3 cluster on mouse chromosome 12qF1.PLoS ONE4e4352
- 120. Georges M, Charlier C, Cockett N (2003) The callipyge locus: evidence for the trans interaction of reciprocally imprinted genes. Trends Genet 19: 248–252.M. GeorgesC. CharlierN. Cockett2003The callipyge locus: evidence for the trans interaction of reciprocally imprinted genes.Trends Genet19248252
- 121. Wermter AK, Scherag A, Meyre D, Reichwald K, Durand E, et al. (2008) Preferential reciprocal transfer of paternal/maternal DLK1 alleles to obese children: first evidence of polar overdominance in humans. Eur J Hum Genet 16: 1126–1134.AK WermterA. ScheragD. MeyreK. ReichwaldE. Durand2008Preferential reciprocal transfer of paternal/maternal DLK1 alleles to obese children: first evidence of polar overdominance in humans.Eur J Hum Genet1611261134
- 122. Davis E, Caiment F, Tordoir X, Cavaille J, Ferguson-Smith A, et al. (2005) RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus. Curr Biol 15: 743–749.E. DavisF. CaimentX. TordoirJ. CavailleA. Ferguson-Smith2005RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus.Curr Biol15743749
- 123. He H, Nagy R, Liyanarachchi S, Jiao H, Li W, et al. (2009) A susceptibility locus for papillary thyroid carcinoma on chromosome 8q24. Cancer Res 69: 625–631.H. HeR. NagyS. LiyanarachchiH. JiaoW. Li2009A susceptibility locus for papillary thyroid carcinoma on chromosome 8q24.Cancer Res69625631
- 124. Duncan I (1987) The bithorax complex. Annu Rev Genet 21: 285–319.I. Duncan1987The bithorax complex.Annu Rev Genet21285319
- 125. Akam ME, Martinez-Arias A (1985) The distribution of Ultrabithorax transcripts in Drosophila embryos. Embo J 4: 1689–1700.ME AkamA. Martinez-Arias1985The distribution of Ultrabithorax transcripts in Drosophila embryos.Embo J416891700
- 126. Lipshitz HD, Peattie DA, Hogness DS (1987) Novel transcripts from the Ultrabithorax domain of the bithorax complex. Genes Dev 1: 307–322.HD LipshitzDA PeattieDS Hogness1987Novel transcripts from the Ultrabithorax domain of the bithorax complex.Genes Dev1307322
- 127. Petruk S, Sedkov Y, Riley KM, Hodgson J, Schweisguth F, et al. (2006) Transcription of bxd noncoding RNAs promoted by trithorax represses Ubx in cis by transcriptional interference. Cell 127: 1209–1221.S. PetrukY. SedkovKM RileyJ. HodgsonF. Schweisguth2006Transcription of bxd noncoding RNAs promoted by trithorax represses Ubx in cis by transcriptional interference.Cell12712091221
- 128. Zhao J, Sun BK, Erwin JA, Song JJ, Lee JT (2008) Polycomb proteins targeted by a short repeat RNA to the mouse X chromosome. Science 322: 750–756.J. ZhaoBK SunJA ErwinJJ SongJT Lee2008Polycomb proteins targeted by a short repeat RNA to the mouse X chromosome.Science322750756
- 129. Sanchez-Herrero E, Akam M (1989) Spatially ordered transcription of regulatory DNA in the bithorax complex of Drosophila. Development 107: 321–329.E. Sanchez-HerreroM. Akam1989Spatially ordered transcription of regulatory DNA in the bithorax complex of Drosophila.Development107321329
- 130. Duncan IW (2002) Transvection effects in Drosophila. Annu Rev Genet 36: 521–556.IW Duncan2002Transvection effects in Drosophila.Annu Rev Genet36521556
- 131. Suemori H, Noguchi S (2000) HoxC cluster genes are dispensable for overall body plan of mouse embryonic development. Dev Biol 220: 333–342.H. SuemoriS. Noguchi2000HoxC cluster genes are dispensable for overall body plan of mouse embryonic development.Dev Biol220333342
- 132. Vaucheret H (2006) Post-transcriptional small RNA pathways in plants: mechanisms and regulations. Genes Dev 20: 759–771.H. Vaucheret2006Post-transcriptional small RNA pathways in plants: mechanisms and regulations.Genes Dev20759771
- 133. Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75: 843–854.RC LeeRL FeinbaumV. Ambros1993The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.Cell75843854
- 134. Wightman B, Ha I, Ruvkun G (1993) Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75: 855–862.B. WightmanI. HaG. Ruvkun1993Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans.Cell75855862
- 135. Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, et al. (2000) The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403: 901–906.BJ ReinhartFJ SlackM. BassonAE PasquinelliJC Bettinger2000The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.Nature403901906
- 136. Hipfner DR, Weigmann K, Cohen SM (2002) The bantam gene regulates Drosophila growth. Genetics 161: 1527–1537.DR HipfnerK. WeigmannSM Cohen2002The bantam gene regulates Drosophila growth.Genetics16115271537
- 137. Brennecke J, Hipfner DR, Stark A, Russell RB, Cohen SM (2003) bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 113: 25–36.J. BrenneckeDR HipfnerA. StarkRB RussellSM Cohen2003bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila.Cell1132536
- 138. Sarin S, O'Meara MM, Flowers EB, Antonio C, Poole RJ, et al. (2007) Genetic screens for Caenorhabditis elegans mutants defective in left/right asymmetric neuronal fate specification. Genetics 176: 2109–2130.S. SarinMM O'MearaEB FlowersC. AntonioRJ Poole2007Genetic screens for Caenorhabditis elegans mutants defective in left/right asymmetric neuronal fate specification.Genetics17621092130
- 139. Pasquinelli AE, Reinhart BJ, Slack F, Martindale MQ, Kuroda MI, et al. (2000) Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature 408: 86–89.AE PasquinelliBJ ReinhartF. SlackMQ MartindaleMI Kuroda2000Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA.Nature4088689
- 140. Johnson SM, Grosshans H, Shingara J, Byrom M, Jarvis R, et al. (2005) RAS is regulated by the let-7 microRNA family. Cell 120: 635–647.SM JohnsonH. GrosshansJ. ShingaraM. ByromR. Jarvis2005RAS is regulated by the let-7 microRNA family.Cell120635647
- 141. Yu F, Yao H, Zhu P, Zhang X, Pan Q, et al. (2007) let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell 131: 1109–1123.F. YuH. YaoP. ZhuX. ZhangQ. Pan2007let-7 regulates self renewal and tumorigenicity of breast cancer cells.Cell13111091123
- 142. Roush S, Slack FJ (2008) The let-7 family of microRNAs. Trends Cell Biol. S. RoushFJ Slack2008The let-7 family of microRNAs.Trends Cell Biol
- 143. Esquela-Kerscher A, Slack FJ (2006) Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer 6: 259–269.A. Esquela-KerscherFJ Slack2006Oncomirs - microRNAs with a role in cancer.Nat Rev Cancer6259269
- 144. Johnston RJ, Hobert O (2003) A microRNA controlling left/right neuronal asymmetry in Caenorhabditis elegans. Nature 426: 845–849.RJ JohnstonO. Hobert2003A microRNA controlling left/right neuronal asymmetry in Caenorhabditis elegans.Nature426845849
- 145. De Gobbi M, Viprakasit V, Hughes JR, Fisher C, Buckle VJ, et al. (2006) A regulatory SNP causes a human genetic disease by creating a new transcriptional promoter. Science 312: 1215–1217.M. De GobbiV. ViprakasitJR HughesC. FisherVJ Buckle2006A regulatory SNP causes a human genetic disease by creating a new transcriptional promoter.Science31212151217
- 146. Libioulle C, Louis E, Hansoul S, Sandor C, Farnir F, et al. (2007) Novel Crohn disease locus identified by genome-wide association maps to a gene desert on 5p13.1 and modulates expression of PTGER4. PLoS Genet 3: e58.C. LibioulleE. LouisS. HansoulC. SandorF. Farnir2007Novel Crohn disease locus identified by genome-wide association maps to a gene desert on 5p13.1 and modulates expression of PTGER4.PLoS Genet3e58
- 147. Marcellini S, Simpson P (2006) Two or four bristles: functional evolution of an enhancer of scute in Drosophilidae. PLoS Biol 4: e386.S. MarcelliniP. Simpson2006Two or four bristles: functional evolution of an enhancer of scute in Drosophilidae.PLoS Biol4e386
- 148. Sipos L, Mihaly J, Karch F, Schedl P, Gausz J, et al. (1998) Transvection in the Drosophila Abd-B domain: extensive upstream sequences are involved in anchoring distant cis-regulatory regions to the promoter. Genetics 149: 1031–1050.L. SiposJ. MihalyF. KarchP. SchedlJ. Gausz1998Transvection in the Drosophila Abd-B domain: extensive upstream sequences are involved in anchoring distant cis-regulatory regions to the promoter.Genetics14910311050
- 149. Hendrickson JE, Sakonju S (1995) Cis and trans interactions between the iab regulatory regions and abdominal-A and abdominal-B in Drosophila melanogaster. Genetics 139: 835–848.JE HendricksonS. Sakonju1995Cis and trans interactions between the iab regulatory regions and abdominal-A and abdominal-B in Drosophila melanogaster.Genetics139835848
- 150. Hopmann R, Duncan D, Duncan I (1995) Transvection in the iab-5,6,7 region of the bithorax complex of Drosophila: homology independent interactions in trans. Genetics 139: 815–833.R. HopmannD. DuncanI. Duncan1995Transvection in the iab-5,6,7 region of the bithorax complex of Drosophila: homology independent interactions in trans.Genetics139815833
- 151. Muller M, Hagstrom K, Gyurkovics H, Pirrotta V, Schedl P (1999) The mcp element from the Drosophila melanogaster bithorax complex mediates long-distance regulatory interactions. Genetics 153: 1333–1356.M. MullerK. HagstromH. GyurkovicsV. PirrottaP. Schedl1999The mcp element from the Drosophila melanogaster bithorax complex mediates long-distance regulatory interactions.Genetics15313331356
- 152. Vazquez J, Muller M, Pirrotta V, Sedat JW (2006) The Mcp element mediates stable long-range chromosome-chromosome interactions in Drosophila. Mol Biol Cell 17: 2158–2165.J. VazquezM. MullerV. PirrottaJW Sedat2006The Mcp element mediates stable long-range chromosome-chromosome interactions in Drosophila.Mol Biol Cell1721582165
- 153. Mattick JS, Gagen MJ (2001) The evolution of controlled multitasked gene networks: the role of introns and other noncoding RNAs in the development of complex organisms. Mol Biol Evol 18: 1611–1630.JS MattickMJ Gagen2001The evolution of controlled multitasked gene networks: the role of introns and other noncoding RNAs in the development of complex organisms.Mol Biol Evol1816111630
- 154. Feng J, Bi C, Clark BS, Mady R, Shah P, et al. (2006) The Evf-2 noncoding RNA is transcribed from the Dlx-5/6 ultraconserved region and functions as a Dlx-2 transcriptional coactivator. Genes Dev 20: 1470–1484.J. FengC. BiBS ClarkR. MadyP. Shah2006The Evf-2 noncoding RNA is transcribed from the Dlx-5/6 ultraconserved region and functions as a Dlx-2 transcriptional coactivator.Genes Dev2014701484
- 155. Pandey RR, Mondal T, Mohammad F, Enroth S, Redrup L, et al. (2008) Kcnq1ot1 antisense noncoding RNA mediates lineage-specific transcriptional silencing through chromatin-level regulation. Mol Cell 32: 232–246.RR PandeyT. MondalF. MohammadS. EnrothL. Redrup2008Kcnq1ot1 antisense noncoding RNA mediates lineage-specific transcriptional silencing through chromatin-level regulation.Mol Cell32232246
- 156. Nagano T, Mitchell JA, Sanz LA, Pauler FM, Ferguson-Smith AC, et al. (2008) The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin. Science 322: 1717–1720.T. NaganoJA MitchellLA SanzFM PaulerAC Ferguson-Smith2008The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin.Science32217171720
- 157. Terranova R, Yokobayashi S, Stadler MB, Otte AP, van Lohuizen M, et al. (2008) Polycomb group proteins Ezh2 and Rnf2 direct genomic contraction and imprinted repression in early mouse embryos. Dev Cell 15: 1–12.R. TerranovaS. YokobayashiMB StadlerAP OtteM. van Lohuizen2008Polycomb group proteins Ezh2 and Rnf2 direct genomic contraction and imprinted repression in early mouse embryos.Dev Cell15112
- 158. Corcoran MM, Hammarsund M, Zhu C, Lerner M, Kapanadze B, et al. (2004) DLEU2 encodes an antisense RNA for the putative bicistronic RFP2/LEU5 gene in humans and mouse. Genes Chromosomes Cancer 40: 285–297.MM CorcoranM. HammarsundC. ZhuM. LernerB. Kapanadze2004DLEU2 encodes an antisense RNA for the putative bicistronic RFP2/LEU5 gene in humans and mouse.Genes Chromosomes Cancer40285297
- 159. Dinger ME, Pang KC, Mercer TR, Mattick JS (2008) Differentiating protein-coding and noncoding RNA: challenges and ambiguities. PLoS Comput Biol 4: e1000176.ME DingerKC PangTR MercerJS Mattick2008Differentiating protein-coding and noncoding RNA: challenges and ambiguities.PLoS Comput Biol4e1000176
- 160. Chamary JV, Parmley JL, Hurst LD (2006) Hearing silence: non-neutral evolution at synonymous sites in mammals. Nat Rev Genet 7: 98–108.JV ChamaryJL ParmleyLD Hurst2006Hearing silence: non-neutral evolution at synonymous sites in mammals.Nat Rev Genet798108
- 161. Davison EJ, Tarpey PS, Fiegler H, Tomlinson IP, Carter NP (2005) Deletion at chromosome band 20p12.1 in colorectal cancer revealed by high resolution array comparative genomic hybridization. Genes Chromosomes Cancer 44: 384–391.EJ DavisonPS TarpeyH. FieglerIP TomlinsonNP Carter2005Deletion at chromosome band 20p12.1 in colorectal cancer revealed by high resolution array comparative genomic hybridization.Genes Chromosomes Cancer44384391
- 162. Dixon AL, Liang L, Moffatt MF, Chen W, Heath S, et al. (2007) A genome-wide association study of global gene expression. Nat Genet 39: 1202–1207.AL DixonL. LiangMF MoffattW. ChenS. Heath2007A genome-wide association study of global gene expression.Nat Genet3912021207
- 163. Jupe ER, Liu XT, Kiehlbauch JL, McClung JK, Dell'Orco RT (1996) Prohibitin in breast cancer cell lines: loss of antiproliferative activity is linked to 3′ untranslated region mutations. Cell Growth Differ 7: 871–878.ER JupeXT LiuJL KiehlbauchJK McClungRT Dell'Orco1996Prohibitin in breast cancer cell lines: loss of antiproliferative activity is linked to 3′ untranslated region mutations.Cell Growth Differ7871878
- 164. Jupe ER, Liu XT, Kiehlbauch JL, McClung JK, Dell'Orco RT (1996) The 3′ untranslated region of prohibitin and cellular immortalization. Exp Cell Res 224: 128–135.ER JupeXT LiuJL KiehlbauchJK McClungRT Dell'Orco1996The 3′ untranslated region of prohibitin and cellular immortalization.Exp Cell Res224128135
- 165. Jenny A, Hachet O, Zavorszky P, Cyrklaff A, Weston MD, et al. (2006) A translation-independent role of oskar RNA in early Drosophila oogenesis. Development 133: 2827–2833.A. JennyO. HachetP. ZavorszkyA. CyrklaffMD Weston2006A translation-independent role of oskar RNA in early Drosophila oogenesis.Development13328272833
- 166. Candeias MM, Malbert-Colas L, Powell DJ, Daskalogianni C, Maslon MM, et al. (2008) p53 mRNA controls p53 activity by managing Mdm2 functions. Nat Cell Biol. MM CandeiasL. Malbert-ColasDJ PowellC. DaskalogianniMM Maslon2008p53 mRNA controls p53 activity by managing Mdm2 functions.Nat Cell Biol
- 167. Rastinejad F, Blau HM (1993) Genetic complementation reveals a novel regulatory role for 3′ untranslated regions in growth and differentiation. Cell 72: 903–917.F. RastinejadHM Blau1993Genetic complementation reveals a novel regulatory role for 3′ untranslated regions in growth and differentiation.Cell72903917
- 168. Rastinejad F, Conboy MJ, Rando TA, Blau HM (1993) Tumor suppression by RNA from the 3′ untranslated region of alpha-tropomyosin. Cell 75: 1107–1117.F. RastinejadMJ ConboyTA RandoHM Blau1993Tumor suppression by RNA from the 3′ untranslated region of alpha-tropomyosin.Cell7511071117
- 169. Fan H, Villegas C, Huang A, Wright JA (1996) Suppression of malignancy by the 3′ untranslated regions of ribonucleotide reductase R1 and R2 messenger RNAs. Cancer Res 56: 4366–4369.H. FanC. VillegasA. HuangJA Wright1996Suppression of malignancy by the 3′ untranslated regions of ribonucleotide reductase R1 and R2 messenger RNAs.Cancer Res5643664369
- 170. Amack JD, Paguio AP, Mahadevan MS (1999) Cis and trans effects of the myotonic dystrophy (DM) mutation in a cell culture model. Hum Mol Genet 8: 1975–1984.JD AmackAP PaguioMS Mahadevan1999Cis and trans effects of the myotonic dystrophy (DM) mutation in a cell culture model.Hum Mol Genet819751984
- 171. Carninci P, Sandelin A, Lenhard B, Katayama S, Shimokawa K, et al. (2006) Genome-wide analysis of mammalian promoter architecture and evolution. Nat Genet 38: 626–635.P. CarninciA. SandelinB. LenhardS. KatayamaK. Shimokawa2006Genome-wide analysis of mammalian promoter architecture and evolution.Nat Genet38626635
- 172. Shirasawa S, Harada H, Furugaki K, Akamizu T, Ishikawa N, et al. (2004) SNPs in the promoter of a B cell-specific antisense transcript, SAS-ZFAT, determine susceptibility to autoimmune thyroid disease. Hum Mol Genet 13: 2221–2231.S. ShirasawaH. HaradaK. FurugakiT. AkamizuN. Ishikawa2004SNPs in the promoter of a B cell-specific antisense transcript, SAS-ZFAT, determine susceptibility to autoimmune thyroid disease.Hum Mol Genet1322212231
- 173. Mette MF, Aufsatz W, van Der Winden J, Matzke MA, Matzke AJ (2000) Transcriptional silencing and promoter methylation triggered by double-stranded RNA. EMBO J 19: 5194–5201.MF MetteW. AufsatzJ. van Der WindenMA MatzkeAJ Matzke2000Transcriptional silencing and promoter methylation triggered by double-stranded RNA.EMBO J1951945201
- 174. Pal-Bhadra M, Bhadra U, Birchler JA (2002) RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila. Mol Cell 9: 315–327.M. Pal-BhadraU. BhadraJA Birchler2002RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila.Mol Cell9315327
- 175. Buhler M, Moazed D (2007) Transcription and RNAi in heterochromatic gene silencing. Nat Struct Mol Biol 14: 1041–1048.M. BuhlerD. Moazed2007Transcription and RNAi in heterochromatic gene silencing.Nat Struct Mol Biol1410411048
- 176. Matzke M, Kanno T, Huettel B, Daxinger L, Matzke AJ (2007) Targets of RNA-directed DNA methylation. Curr Opin Plant Biol 10: 512–519.M. MatzkeT. KannoB. HuettelL. DaxingerAJ Matzke2007Targets of RNA-directed DNA methylation.Curr Opin Plant Biol10512519
- 177. Pal-Bhadra M, Leibovitch BA, Gandhi SG, Rao M, Bhadra U, et al. (2004) Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery. Science 303: 669–672.M. Pal-BhadraBA LeibovitchSG GandhiM. RaoU. Bhadra2004Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery.Science303669672
- 178. Singh J, Freeling M, Lisch D (2008) A position effect on the heritability of epigenetic silencing. PLoS Genet 4: e1000216.J. SinghM. FreelingD. Lisch2008A position effect on the heritability of epigenetic silencing.PLoS Genet4e1000216
- 179. Brennecke J, Malone CD, Aravin AA, Sachidanandam R, Stark A, et al. (2008) An epigenetic role for maternally inherited piRNAs in transposon silencing. Science 322: 1387–1392.J. BrenneckeCD MaloneAA AravinR. SachidanandamA. Stark2008An epigenetic role for maternally inherited piRNAs in transposon silencing.Science32213871392
- 180. Yang PK, Kuroda MI (2007) Noncoding RNAs and intranuclear positioning in monoallelic gene expression. Cell 128: 777–786.PK YangMI Kuroda2007Noncoding RNAs and intranuclear positioning in monoallelic gene expression.Cell128777786
- 181. Sasaki H, Matsui Y (2008) Epigenetic events in mammalian germ-cell development: reprogramming and beyond. Nat Rev Genet 9: 129–140.H. SasakiY. Matsui2008Epigenetic events in mammalian germ-cell development: reprogramming and beyond.Nat Rev Genet9129140
- 182. Chandler VL (2007) Paramutation: from maize to mice. Cell 128: 641–645.VL Chandler2007Paramutation: from maize to mice.Cell128641645
- 183. Cuzin F, Grandjean V, Rassoulzadegan M (2008) Inherited variation at the epigenetic level: paramutation from the plant to the mouse. Curr Opin Genet Dev 18: 193–196.F. CuzinV. GrandjeanM. Rassoulzadegan2008Inherited variation at the epigenetic level: paramutation from the plant to the mouse.Curr Opin Genet Dev18193196
- 184. Costa FF (2008) Non-coding RNAs, epigenetics and complexity. Gene 410: 9–17.FF Costa2008Non-coding RNAs, epigenetics and complexity.Gene410917
- 185. Osborne RJ, Thornton CA (2006) RNA-dominant diseases. Hum Mol Genet 15: R162–169.RJ OsborneCA Thornton2006RNA-dominant diseases.Hum Mol Genet15R162169
- 186. Angeloni D, Ter Elst A, Wei MH, van der Veen AY, Braga EA, et al. (2006) Analysis of a new homozygous deletion in the tumor suppressor region at 3p12.3 reveals two novel intronic noncoding RNA genes. Genes Chromosomes Cancer 45: 676–691.D. AngeloniA. Ter ElstMH WeiAY van der VeenEA Braga2006Analysis of a new homozygous deletion in the tumor suppressor region at 3p12.3 reveals two novel intronic noncoding RNA genes.Genes Chromosomes Cancer45676691
- 187. Dallosso AR, Hancock AL, Malik S, Salpekar A, King-Underwood L, et al. (2007) Alternately spliced WT1 antisense transcripts interact with WT1 sense RNA and show epigenetic and splicing defects in cancer. Rna 13: 2287–2299.AR DallossoAL HancockS. MalikA. SalpekarL. King-Underwood2007Alternately spliced WT1 antisense transcripts interact with WT1 sense RNA and show epigenetic and splicing defects in cancer.Rna1322872299
- 188. Ishii N, Ozaki K, Sato H, Mizuno H, Saito S, et al. (2006) Identification of a novel non-coding RNA, MIAT, that confers risk of myocardial infarction. J Hum Genet 51: 1087–1099.N. IshiiK. OzakiH. SatoH. MizunoS. Saito2006Identification of a novel non-coding RNA, MIAT, that confers risk of myocardial infarction.J Hum Genet5110871099
- 189. Sleutels F, Zwart R, Barlow DP (2002) The non-coding Air RNA is required for silencing autosomal imprinted genes. Nature 415: 810–813.F. SleutelsR. ZwartDP Barlow2002The non-coding Air RNA is required for silencing autosomal imprinted genes.Nature415810813
- 190. Ding F, Prints Y, Dhar MS, Johnson DK, Garnacho-Montero C, et al. (2005) Lack of Pwcr1/MBII-85 snoRNA is critical for neonatal lethality in Prader-Willi syndrome mouse models. Mamm Genome 16: 424–431.F. DingY. PrintsMS DharDK JohnsonC. Garnacho-Montero2005Lack of Pwcr1/MBII-85 snoRNA is critical for neonatal lethality in Prader-Willi syndrome mouse models.Mamm Genome16424431
- 191. Sahoo T, del Gaudio D, German JR, Shinawi M, Peters SU, et al. (2008) Prader-Willi phenotype caused by paternal deficiency for the HBII-85 C/D box small nucleolar RNA cluster. Nat Genet 40: 719–721.T. SahooD. del GaudioJR GermanM. ShinawiSU Peters2008Prader-Willi phenotype caused by paternal deficiency for the HBII-85 C/D box small nucleolar RNA cluster.Nat Genet40719721
- 192. Zhou Y, Zhong Y, Wang Y, Zhang X, Batista DL, et al. (2007) Activation of p53 by MEG3 non-coding RNA. J Biol Chem 282: 24731–24742.Y. ZhouY. ZhongY. WangX. ZhangDL Batista2007Activation of p53 by MEG3 non-coding RNA.J Biol Chem2822473124742
- 193. Tanaka R, Satoh H, Moriyama M, Satoh K, Morishita Y, et al. (2000) Intronic U50 small-nucleolar-RNA (snoRNA) host gene of no protein-coding potential is mapped at the chromosome breakpoint t(3;6)(q27;q15) of human B-cell lymphoma. Genes Cells 5: 277–287.R. TanakaH. SatohM. MoriyamaK. SatohY. Morishita2000Intronic U50 small-nucleolar-RNA (snoRNA) host gene of no protein-coding potential is mapped at the chromosome breakpoint t(3;6)(q27;q15) of human B-cell lymphoma.Genes Cells5277287
- 194. Millar JK, Wilson-Annan JC, Anderson S, Christie S, Taylor MS, et al. (2000) Disruption of two novel genes by a translocation co-segregating with schizophrenia. Human Mol Genet 9: 1415–1423.JK MillarJC Wilson-AnnanS. AndersonS. ChristieMS Taylor2000Disruption of two novel genes by a translocation co-segregating with schizophrenia.Human Mol Genet914151423
- 195. Tufarelli C, Stanley JA, Garrick D, Sharpe JA, Ayyub H, et al. (2003) Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease. Nat Genet 34: 157–165.C. TufarelliJA StanleyD. GarrickJA SharpeH. Ayyub2003Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease.Nat Genet34157165
- 196. Seidl CI, Stricker SH, Barlow DP (2006) The imprinted Air ncRNA is an atypical RNAPII transcript that evades splicing and escapes nuclear export. Embo J 25: 3565–3575.CI SeidlSH StrickerDP Barlow2006The imprinted Air ncRNA is an atypical RNAPII transcript that evades splicing and escapes nuclear export.Embo J2535653575
- 197. Faghihi MA, Modarresi F, Khalil AM, Wood DE, Sahagan BG, et al. (2008) Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of beta-secretase. Nat Med 14: 723–730.MA FaghihiF. ModarresiAM KhalilDE WoodBG Sahagan2008Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of beta-secretase.Nat Med14723730
- 198. Lin D, Pestova TV, Hellen CU, Tiedge H (2008) Translational control by a small RNA: dendritic BC1 RNA targets the eukaryotic initiation factor 4A helicase mechanism. Mol Cell Biol 28: 3008–3019.D. LinTV PestovaCU HellenH. Tiedge2008Translational control by a small RNA: dendritic BC1 RNA targets the eukaryotic initiation factor 4A helicase mechanism.Mol Cell Biol2830083019
- 199. Takeda K, Ichijo H, Fujii M, Mochida Y, Saitoh M, et al. (1998) Identification of a novel bone morphogenetic protein-responsive gene that may function as a noncoding RNA. J Biol Chem 273: 17079–17085.K. TakedaH. IchijoM. FujiiY. MochidaM. Saitoh1998Identification of a novel bone morphogenetic protein-responsive gene that may function as a noncoding RNA.J Biol Chem2731707917085
- 200. Wang X, Arai S, Song X, Reichart D, Du K, et al. (2008) Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription. Nature 454: 126–130.X. WangS. AraiX. SongD. ReichartK. Du2008Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription.Nature454126130
- 201. Tsang WP, Wong TW, Cheung AH, Co CN, Kwok TT (2007) Induction of drug resistance and transformation in human cancer cells by the noncoding RNA CUDR. Rna 13: 890–898.WP TsangTW WongAH CheungCN CoTT Kwok2007Induction of drug resistance and transformation in human cancer cells by the noncoding RNA CUDR.Rna13890898
- 202. Wagner LA, Christensen CJ, Dunn DM, Spangrude GJ, Georgelas A, et al. (2007) EGO, a novel, non-coding RNA gene, regulates eosinophil granule protein transcript expression. Blood 109: 5191–5198.LA WagnerCJ ChristensenDM DunnGJ SpangrudeA. Georgelas2007EGO, a novel, non-coding RNA gene, regulates eosinophil granule protein transcript expression.Blood10951915198
- 203. Brookheart RT, Michel CI, Listenberger LL, Ory DS, Schaffer JE (2009) The non-coding RNA gadd7 is a regulator of lipid-induced oxidative and ER stress. J Biol Chem 284: 7446–7454.RT BrookheartCI MichelLL ListenbergerDS OryJE Schaffer2009The non-coding RNA gadd7 is a regulator of lipid-induced oxidative and ER stress.J Biol Chem28474467454
- 204. Mourtada-Maarabouni M, Hedge VL, Kirkham L, Farzaneh F, Williams GT (2008) Growth arrest in human T-cells is controlled by the non-coding RNA growth-arrest-specific transcript 5 (GAS5). J Cell Sci 121: 939–946.M. Mourtada-MaarabouniVL HedgeL. KirkhamF. FarzanehGT Williams2008Growth arrest in human T-cells is controlled by the non-coding RNA growth-arrest-specific transcript 5 (GAS5).J Cell Sci121939946
- 205. Matouk IJ, DeGroot N, Mezan S, Ayesh S, Abu-lail R, et al. (2007) The H19 non-coding RNA is essential for human tumor growth. PLoS ONE 2: e845.IJ MatoukN. DeGrootS. MezanS. AyeshR. Abu-lail2007The H19 non-coding RNA is essential for human tumor growth.PLoS ONE2e845
- 206. Gabory A, Ripoche MA, Yoshimizu T, Dandolo L (2006) The H19 gene: regulation and function of a non-coding RNA. Cytogenet Genome Res 113: 188–193.A. GaboryMA RipocheT. YoshimizuL. Dandolo2006The H19 gene: regulation and function of a non-coding RNA.Cytogenet Genome Res113188193
- 207. Yoshimizu T, Miroglio A, Ripoche MA, Gabory A, Vernucci M, et al. (2008) The H19 locus acts in vivo as a tumor suppressor. Proc Natl Acad Sci U S A 105: 12417–12422.T. YoshimizuA. MiroglioMA RipocheA. GaboryM. Vernucci2008The H19 locus acts in vivo as a tumor suppressor.Proc Natl Acad Sci U S A1051241712422
- 208. Shamovsky I, Ivannikov M, Kandel ES, Gershon D, Nudler E (2006) RNA-mediated response to heat shock in mammalian cells. Nature 440: 556–560.I. ShamovskyM. IvannikovES KandelD. GershonE. Nudler2006RNA-mediated response to heat shock in mammalian cells.Nature440556560
- 209. Mayer C, Schmitz KM, Li J, Grummt I, Santoro R (2006) Intergenic transcripts regulate the epigenetic state of rRNA genes. Mol Cell 22: 351–361.C. MayerKM SchmitzJ. LiI. GrummtR. Santoro2006Intergenic transcripts regulate the epigenetic state of rRNA genes.Mol Cell22351361
- 210. Thakur N, Tiwari VK, Thomassin H, Pandey RR, Kanduri M, et al. (2004) An antisense RNA regulates the bidirectional silencing property of the Kcnq1 imprinting control region. Mol Cell Biol 24: 7855–7862.N. ThakurVK TiwariH. ThomassinRR PandeyM. Kanduri2004An antisense RNA regulates the bidirectional silencing property of the Kcnq1 imprinting control region.Mol Cell Biol2478557862
- 211. Imamura T, Yamamoto S, Ohgane J, Hattori N, Tanaka S, et al. (2004) Non-coding RNA directed DNA demethylation of Sphk1 CpG island. Biochem Biophys Res Commun 322: 593–600.T. ImamuraS. YamamotoJ. OhganeN. HattoriS. Tanaka2004Non-coding RNA directed DNA demethylation of Sphk1 CpG island.Biochem Biophys Res Commun322593600
- 212. Madamanchi NR, Hu ZY, Li F, Horaist C, Moon SK, et al. (2002) A noncoding RNA regulates human protease-activated receptor-1 gene during embryogenesis. Biochim Biophys Acta 1576: 237–245.NR MadamanchiZY HuF. LiC. HoraistSK Moon2002A noncoding RNA regulates human protease-activated receptor-1 gene during embryogenesis.Biochim Biophys Acta1576237245
- 213. Tochitani S, Hayashizaki Y (2008) Nkx2.2 antisense RNA overexpression enhanced oligodendrocytic differentiation. Biochem Biophys Res Commun 372: 691–696.S. TochitaniY. Hayashizaki2008Nkx2.2 antisense RNA overexpression enhanced oligodendrocytic differentiation.Biochem Biophys Res Commun372691696
- 214. Willingham AT, Orth AP, Batalov S, Peters EC, Wen BG, et al. (2005) A strategy for probing the function of noncoding RNAs finds a repressor of NFAT. Science 309: 1570–1573.AT WillinghamAP OrthS. BatalovEC PetersBG Wen2005A strategy for probing the function of noncoding RNAs finds a repressor of NFAT.Science30915701573
- 215. Fu X, Ravindranath L, Tran N, Petrovics G, Srivastava S (2006) Regulation of apoptosis by a prostate-specific and prostate cancer-associated noncoding gene, PCGEM1. DNA Cell Biol 25: 135–141.X. FuL. RavindranathN. TranG. PetrovicsS. Srivastava2006Regulation of apoptosis by a prostate-specific and prostate cancer-associated noncoding gene, PCGEM1.DNA Cell Biol25135141
- 216. Ginger MR, Shore AN, Contreras A, Rijnkels M, Miller J, et al. (2006) A noncoding RNA is a potential marker of cell fate during mammary gland development. Proc Natl Acad Sci U S A 103: 5781–5786.MR GingerAN ShoreA. ContrerasM. RijnkelsJ. Miller2006A noncoding RNA is a potential marker of cell fate during mammary gland development.Proc Natl Acad Sci U S A10357815786
- 217. Yan MD, Hong CC, Lai GM, Cheng AL, Lin YW, et al. (2005) Identification and characterization of a novel gene Saf transcribed from the opposite strand of Fas. Hum Mol Genet 14: 1465–1474.MD YanCC HongGM LaiAL ChengYW Lin2005Identification and characterization of a novel gene Saf transcribed from the opposite strand of Fas.Hum Mol Genet1414651474
- 218. Valgardsdottir R, Chiodi I, Giordano M, Cobianchi F, Riva S, et al. (2005) Structural and functional characterization of noncoding repetitive RNAs transcribed in stressed human cells. Mol Biol Cell 16: 2597–2604.R. ValgardsdottirI. ChiodiM. GiordanoF. CobianchiS. Riva2005Structural and functional characterization of noncoding repetitive RNAs transcribed in stressed human cells.Mol Biol Cell1625972604
- 219. Azzalin CM, Reichenbach P, Khoriauli L, Giulotto E, Lingner J (2007) Telomeric repeat containing RNA and RNA surveillance factors at mammalian chromosome ends. Science 318: 798–801.CM AzzalinP. ReichenbachL. KhoriauliE. GiulottoJ. Lingner2007Telomeric repeat containing RNA and RNA surveillance factors at mammalian chromosome ends.Science318798801
- 220. Schoeftner S, Blasco MA (2008) Developmentally regulated transcription of mammalian telomeres by DNA-dependent RNA polymerase II. Nat Cell Biol 10: 228–236.S. SchoeftnerMA Blasco2008Developmentally regulated transcription of mammalian telomeres by DNA-dependent RNA polymerase II.Nat Cell Biol10228236
- 221. Lee JT, Davidow LS, Warshawsky D (1999) Tsix, a gene antisense to Xist at the X-inactivation centre. Nature Genet 21: 400–404.JT LeeLS DavidowD. Warshawsky1999Tsix, a gene antisense to Xist at the X-inactivation centre.Nature Genet21400404
- 222. Luikenhuis S, Wutz A, Jaenisch R (2001) Antisense transcription through the Xist locus mediates Tsix function in embryonic stem cells. Mol Cell Biol 21: 8512–8520.S. LuikenhuisA. WutzR. Jaenisch2001Antisense transcription through the Xist locus mediates Tsix function in embryonic stem cells.Mol Cell Biol2185128520
- 223. Sado T, Hoki Y, Sasaki H (2005) Tsix silences Xist through modification of chromatin structure. Dev Cell 9: 159–165.T. SadoY. HokiH. Sasaki2005Tsix silences Xist through modification of chromatin structure.Dev Cell9159165
- 224. Wang F, Li X, Xie X, Zhao L, Chen W (2008) UCA1, a non-protein-coding RNA up-regulated in bladder carcinoma and embryo, influencing cell growth and promoting invasion. FEBS Lett 582: 1919–1927.F. WangX. LiX. XieL. ZhaoW. Chen2008UCA1, a non-protein-coding RNA up-regulated in bladder carcinoma and embryo, influencing cell growth and promoting invasion.FEBS Lett58219191927
- 225. Sim S, Weinberg DE, Fuchs G, Choi K, Chung J, et al. (2009) The subcellular distribution of an RNA quality control protein, the ro autoantigen, is regulated by noncoding y RNA binding. Mol Biol Cell 20: 1555–1564.S. SimDE WeinbergG. FuchsK. ChoiJ. Chung2009The subcellular distribution of an RNA quality control protein, the ro autoantigen, is regulated by noncoding y RNA binding.Mol Biol Cell2015551564
- 226. Beltran M, Puig I, Pena C, Garcia JM, Alvarez AB, et al. (2008) A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition. Genes Dev 22: 756–769.M. BeltranI. PuigC. PenaJM GarciaAB Alvarez2008A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition.Genes Dev22756769
- 227. Komine Y, Nakamura K, Katsuki M, Yamamori T (2006) Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain. Mol Cell Neurosci 31: 273–283.Y. KomineK. NakamuraM. KatsukiT. Yamamori2006Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain.Mol Cell Neurosci31273283
- 228. Katayama S, Tomaru Y, Kasukawa T, Waki K, Nakanishi M, et al. (2005) Antisense transcription in the mammalian transcriptome. Science 309: 1564–1566.S. KatayamaY. TomaruT. KasukawaK. WakiM. Nakanishi2005Antisense transcription in the mammalian transcriptome.Science30915641566
- 229. Toledo-Arana A, Repoila F, Cossart P (2007) Small noncoding RNAs controlling pathogenesis. Curr Opin Microbiol 10: 182–188.A. Toledo-AranaF. RepoilaP. Cossart2007Small noncoding RNAs controlling pathogenesis.Curr Opin Microbiol10182188
- 230. Pollard KS, Salama SR, Lambert N, Lambot MA, Coppens S, et al. (2006) An RNA gene expressed during cortical development evolved rapidly in humans. Nature 443: 167–172.KS PollardSR SalamaN. LambertMA LambotS. Coppens2006An RNA gene expressed during cortical development evolved rapidly in humans.Nature443167172