Submarine hydrothermal vents are model systems for the Archaean Earth environment, and some sites maintain conditions that may have favored the formation and evolution of cellular life. Vents are typified by rapid fluctuations in temperature and redox potential that impose a strong selective pressure on resident microbial communities. Nautilia profundicola strain Am-H is a moderately thermophilic, deeply-branching Epsilonproteobacterium found free-living at hydrothermal vents and is a member of the microbial mass on the dorsal surface of vent polychaete, Alvinella pompejana. Analysis of the 1.7-Mbp genome of N. profundicola uncovered adaptations to the vent environment—some unique and some shared with other Epsilonproteobacterial genomes. The major findings included: (1) a diverse suite of hydrogenases coupled to a relatively simple electron transport chain, (2) numerous stress response systems, (3) a novel predicted nitrate assimilation pathway with hydroxylamine as a key intermediate, and (4) a gene (rgy) encoding the hallmark protein for hyperthermophilic growth, reverse gyrase. Additional experiments indicated that expression of rgy in strain Am-H was induced over 100-fold with a 20°C increase above the optimal growth temperature of this bacterium and that closely related rgy genes are present and expressed in bacterial communities residing in geographically distinct thermophilic environments. N. profundicola, therefore, is a model Epsilonproteobacterium that contains all the genes necessary for life in the extreme conditions widely believed to reflect those in the Archaean biosphere—anaerobic, sulfur, H2- and CO2-rich, with fluctuating redox potentials and temperatures. In addition, reverse gyrase appears to be an important and common adaptation for mesophiles and moderate thermophiles that inhabit ecological niches characterized by rapid and frequent temperature fluctuations and, as such, can no longer be considered a unique feature of hyperthermophiles.
Extreme environments, such as deep-sea hydrothermal vents, found 2,500 meters below the ocean surface, support large macrofaunal communities via microbially mediated carbon fixation processes using chemicals (chemoautotrophy) rather than light (photoautotrophy). The genome of one such model chemoautotrophic microbe, N. profundicola, was sequenced and described in this work. N. profundicola, distantly related to the pathogenic Helicobacter and Campylobacter species, contains a number of genes and pathways predicted to be important in DNA repair, environmental sensing, and metabolism, which are novel to either its subdivision or to all microbes. The genes and deduced metabolic pathways include several hydrogen uptake and release systems as well as a novel predicted nitrogen assimilation pathway. One gene involved in DNA repair, reverse gyrase, was thought to be a hallmark protein in hyperthermophiles, which are microbes that grow above 80°C. We found this gene to be highly expressed at 65°C, over 20°C above the optimal growth temperature of this organism. Therefore, the genome of this model deep-sea hydrothermal vent chemoautotroph may reflect what is required for life in an extreme environment, hypothesized to be similar to early earth conditions.
Citation: Campbell BJ, Smith JL, Hanson TE, Klotz MG, Stein LY, Lee CK, et al. (2009) Adaptations to Submarine Hydrothermal Environments Exemplified by the Genome of Nautilia profundicola. PLoS Genet5(2): e1000362. https://doi.org/10.1371/journal.pgen.1000362
Editor: Claire M. Fraser-Liggett, Institute for Genome Sciences, University of Maryland School of Medicine, United States of America
Received: August 14, 2008; Accepted: December 31, 2008; Published: February 6, 2009
Copyright: © 2009 Campbell et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by an NSF grants to BJC, SCC and JAE (EF-0333203) and to BJC (DEB-0640414). TEH was supported by CAREER award MCB-0447649 from NSF. MGK was supported by NSF grant EF-0412129. LYS was supported by the UCR Ag. Exp. Sta.
Competing interests: The authors have declared that no competing interests exist.
Food webs at deep-sea hydrothermal vents are based on microbial primary productivity fueled by chemical reactions rather than light. Microorganisms that thrive in these environments must adapt to fluctuations in temperature and redox conditions, ranging from the hot, sulfidic, heavy metal-laden plume at the vent outlet to cold, oxic seawater in the surrounding region –. In addition, DNA damaging agents such as ionizing radiation add to the harsh conditions with which hydrothermal residents must contend –. While a variety of diverse organisms have been isolated from hydrothermal environments ,, it is clear from molecular surveys and in situ hybridization studies that Epsilonproteobacteria are numerically dominant and therefore likely key players in the cycling of C, N, and S at deep-sea hydrothermal vents .
Vent-associated Epsilonproteobacteria are diverse, including the deeply-branching Nautiliales and Nitratiruptor groups, Sulfurospirillum, Arcobacter, and the “Thiovulgaceae” Marine Groups I and II ,. Molecular and culture-based surveys have demonstrated Epsilonproteobacteria in diverse sub-seafloor habitats –. Two complete genome sequences of cultured vent Epsilonproteobacteria are available, Nitratiruptor sp. SB155-2 and Sulfurovum sp. NBC37-1 , while that of Caminibacter mediatlanticus, isolated from the Mid-Atlantic Ridge  has been sequenced to draft level. We describe here the 1.7 Mbp genome of Nautilia profundicola strain Am-H, the first from a member of the order Nautiliales isolated from hydrothermal vents ,.
N. profundicola strain Am-H was originally isolated from the biomass of an Alvinella pompejana episymbiont community collected at 13°N along the East Pacific Rise Axial Caldera ,. Strain Am-H is only found in some episymbiont communities and is readily detected as a free-living organism in various mats on vent chimneys . N. profundicola falls within the Nautiliales order of the Epsilonproteobacteria class, the deepest branching order of this subdivision , – and is closely related to other vent isolates: Caminibacter hydrogeniphilus and Nautilia lithotrophica ,. All are moderately thermophilic strict anaerobes that grow lithoautotrophically with H2 and elemental sulfur (S0). Most likely, all members of this group utilize the reductive or reverse tricarboxylic acid (rTCA) cycle for CO2 fixation ,,. N. profundicola and relatives differ from vent isolates whose genomes have been sequenced, including Thiomicrospira crunogena (Gammaproteobacteria), Nitratiruptor sp. SB155-2 (Epsilonproteobacteria), and Sulfurovum sp. NBC37-1 (Epsilonproteobacteria) ,. These are sulfur-oxidizing microaerophiles that fix CO2 via the Calvin-Benson-Bassham cycle (T. crunogena) or the rTCA cycle (Nitratiruptor sp. SB155-2, Sulfurovum sp. NBC37-1) , –. Am-H and N. lithotrophica are differentiated from C. hydrogeniphilus as well as other Epsilonproteobacteria (Caminibacter mediatlanticus, Wolinella succinogenes, and Sulfuromonas denitrificans) by the inability to respire nitrate ,,,. However, strain Am-H and N. lithotrophica can both utilize nitrate as the sole source of nitrogen ,.
The large temperature and redox variations present in diffuse hydrothermal flow regimes, which may be extant examples of an early life environment , impose strong selective pressures for characteristic adaptations in resident microbial communities. Determining these exact mechanisms is one of the most compelling issues for vent microbiologists and others interested in adaptations to life in extreme environments. Prior surveys found that typical DNA-repair genes are absent from the Nitratiruptor sp. SB155-2 and Sulfurovum sp. NBC37-1 genomes , perhaps indicating that Epsilonproteobacteria genomes can rapidly change, which may confer a selective advantage in either pathogenic associations or deep-sea hydrothermal vent environments ,,. However, this flexibility must be balanced against DNA damage–inducing conditions present at deep-sea hydrothermal vents, including high, fluctuating temperatures and ionizing radiation.
A comparison of the N. profundicola genome to the genomes of other hydrothermal vent bacteria has suggested several common features of a hydrothermally adapted lifestyle. One example of such adaptation, a reverse gyrase, was further examined in terms of regulated expression in N. profundicola, distribution among other Epsilonproteobacteria, and expression in deep- sea hydrothermal environments. In addition, its genome sequence indicates that strain Am-H utilizes nitrate in the absence of a canonical nitrite reductase and instead likely relies on a novel pathway where hydroxylamine is a key intermediate. Our analyses suggest that not only is N. profundicola the deepest branching epsilonproteobacterial genome to be sequenced to date, but its genome may provide evolutionary insights beyond what is required for growth at hydrothermal vents.
General Genome Properties
The genome of N. profundicola (GenBank accession number, CP001279) is a single circular chromosome of 1,676,444 bp containing 1,745 protein-coding genes (CDSs); a small genome compared to other free-living Epsilonproteobacteria ,. The compactness of the genome is reflected in the absence of duplicated gene clusters. The genome's general features are summarized in Figure 1 and Table 1. Other aspects of the isolate and methods are detailed in Table S1. Basepair 1 was assigned upstream of dnaA, the chromosomal replication initiator protein (GAmH_0001). The origin of replication is likely to be located in this area based on the shift in GC skew pattern . The sequences of genes in the four rRNA operons are 100% identical even through the intergenic regions. The genome contains the most tRNA genes (48) of all the sequenced Epsilonproteobacteria genomes, including one corresponding to selenocysteine (Table 1). To date, 21.5% of bacterial genomes contains at least one clearly identified selenocysteine-dependent enzyme .
Circles correspond to the following features, starting with the outermost circle: (1) forward strand genes (2) reverse strand genes (3) χ2 deviation of local nucleotide composition from the genome average (4) GC skew (5) tRNAs (green lines) (6) rRNAs (blue lines) (7) small RNAs (red lines). Genes are colored according to their role categories.
There is little evidence of recent widespread horizontal gene transfer (HGT) events into the genome of N. profundicola, based on % G+C ratio or tetranucleotide frequency anomalies (Figure 1). While no extrachromosomal elements were found, the genome contains several features that may indicate the existence of mobile genetic elements. The chromosome contains a group of genes annotated as a plasmid stabilization system gene (GAmH_0503) and a toxin/antitoxin gene pair (GAmH_0504/502) most closely related to the relBE-3 family of toxin/antitoxins ,. This family, when found on plasmids, prevents plasmid loss during replication. Integrated into the genome, they are predicted as stress response factors to starvation or other adverse conditions by inducing cell cycle arrest or programmed cell death . The lack of homologous genes in other Epsilonproteobacteria suggests a recent HGT event to N. profundicola. No typical transposons were identified in the genome, although there is one potential transposase (GAmH_0909) with similarity to a gene in C. mediatlanticus, which also lacks other identifiable transposases. This contrasts with Nitratiruptor sp. SB155-2 and Sulfurovum sp. NBC37-1 genomes, which contain 5 and 14 predicted transposases, respectively . There are no apparent full length prophages, although there are four genes identified as phage integrases, three of which are putative site-specific recombinases (GAmH_0249, 1143, 0597, XerD), and one (GAmH_1472) with no significant similarities to genes in GenBank (e-value >0.005). One potential prophage, around 10 Kb in length, was identified using Prophage Finder . This putative prophage spans 15 coding sequences (GAmH_1141-1155), including one of the phage integrases (GAmH_1143) and nine hypothetical proteins. Also in this region are a zonular occludens toxin (Zot) family protein (GAmH_1150), a bacteriophage replication gene A protein (GPA) (GAmH_1152), a putative cupin domain protein (GAmH_1153), a putative tetratrico peptide repeat (GAmH_1154), and DNA primase (GAmH_1155). The similarity of genes in this region to those found in other Epsilonproteobacteria indicates either an ancient phage integration event into an ancestral genome or that similar viruses infect the subdivision. Two tRNAs are coded within this potential prophage region, one of which is the only glutamine tRNA in the genome. The apparent lack of foreign DNA may be due to yet undescribed defensive mechanisms in N. profundicola or to the harsh external environment, which could select against the persistence of free DNA.
Free-living bacteria must sense and respond to environmental changes quickly and do so through a variety of signal transduction mechanisms. Pathogenic species have fewer signal transduction proteins than their free-living counterparts, even when genome size is taken into account , as is the case among sequenced Epsilonproteobacteria. N. profundicola, Nitratiruptor sp. SB155-2, and Sulfurovum sp. NBC37-1 each have over 100 signal transduction genes, while most strains of Campylobacter and Helicobacter have about half as many, most of which code for histidine kinases, response regulators, or methyl-accepting chemotaxis proteins. Free-living Epsilonproteobacteria have many genes containing diguanylate cyclase (GGDEF) and phosphodiesterase (EAL) domains, whereas Campylobacter and Helicobacter genomes have only a few or none.
Among vent Epsilonproteobacteria, N. profundicola has the most stand-alone GGDEF and EAL domain family protein gene homologs, while Nitratiruptor sp. SB155-2 and Sulfurovum sp. NBC37-1 have more gene homologs belonging to a predicted signal transduction protein family containing a membrane domain as well as GGDEF and EAL domains (Table 2). N. profundicola is the only vent Epsilonproteobacterium that also has the HD-GYP domain, another phosphodiesterase. Given its smaller genome size and comparatively limited metabolism, its numerous coding sequences for cyclic diguanylate signaling relative to other vent organisms seems counterintuitive. Perhaps having more of these intracellular signaling molecules allows N. profundicola to more precisely or rapidly regulate protein expression in the highly variable vent environment. Then again, this may also reflect N. profundicola's changing lifestyles between a free-living existence and being associated with a complex metazoan-microbial symbiosis system which is essentially a biofilm, and the different requirements for gene expression under these two conditions.
The sheer abundance of GGDEF and EAL domains within many genomes, including N. profundicola, suggests that a wide variety of environmental stimuli may be detected by these domains . Both the two-component and c-diGMP signal transduction systems sense and respond to periplasmic substrates, and N. profundicola has GGDEF or EAL domains associated regions that bind amino acids (PBPb domain) or carbohydrates (CelB domain). In addition, as a strict anaerobe, N. profundicola requires the ability to detect oxygen and move away from it, and unsurprisingly possesses a PAS domain (common domain in Period circadian protein, Aryl hydrocarbon receptor nuclear translocator, and Single-minded protein) protein (COG 2202) that senses redox conditions and binds gaseous molecules like NO, CO, and O2 . Known outputs of the c-diGMP system include changes in motility, virulence, heavy metal resistance, phage resistance, cell to cell communication, exopolysaccharide production, and biofilm formation ,. In the hydrothermal vent environment, N. profundicola would benefit from the regulation of motility and heavy metal resistance through c-diGMP molecules. In addition, regulation of phage resistance could be particularly important to Epsilonproteobacteria which lack traditional DNA repair systems.
Cell Sensing Systems
Nautilia profundicola has homologs of the autoinducer-2 (AI-2) system of quorum sensing, S-ribosylhomocysteinase (luxS) (GAmH_0429), and 5′methylthioadenosine/S-adenosylhomocysteine nucleosidase (pfs) (GAmH_0316). At the time of writing, 22 out of 26 sequenced Epsilonproteobacteria have the luxS and pfs genes, with only four Campylobacter species lacking the AI-2 system. While LuxS has been shown to affect biofilm formation, motility, type III secretion, and the production of virulence factors and toxins in pathogens, it remains unclear whether AI-2 is truly a universal signal or simply a metabolic byproduct for most bacterial strains –. In support of the widespread nature of LuxS/Pfs in Epsilonproteobacteria, the metagenome of the A. pompejana episymbionts contains 36 luxS sequences and 49 pfs sequences . The abundance of these genes in Epsilonproteobacteria at hydrothermal vents may offer a mechanism through which this group dominates episymbiont communities. Alternatively and perhaps more likely, quorum sensing at hydrothermal vents could be used for diffusion sensing . The ability to detect diffusion rates in highly variable flow regimes could allow N. profundicola to regulate secretion of extracellular enzymes. Since the AI-2 inducer is a metabolic byproduct, loss of AI-2 to the environment is less energetically costly than the loss of an enzyme.
Biofilm-Related Pathways: Polyamine, Flagellar, and Pilin Syntheses
N. profundicola likely maintains a mostly attached lifestyle due to the dynamic fluid flow and mixing occurring at vents. It is associated with some, but not all, episymbiont communities of the hydrothermal vent polychaete, A. pompejana, and is also associated with bacterial mats on chimney surfaces . Polyamines have been shown to play significant roles in the formation of biofilms in Yersinia pestis, the causative agent of bubonic plague . N. profundicola appears to be similar to Y. pestis and H. pylori in polyamine synthesis pathways ,. Genes encoding arginine decarboxylase (speA, GAmH_0346) and spermidine synthase (speE, GAmH_0033) are found in the N. profundicola genome for spermidine formation, but the conversion from agmatine to putrescine is likely performed jointly by agmatine deiminase (aguA, GAmH_1708) and N-carbamoylputrescine amidohydrolase (aguB, GAmH_0211) instead of agmatinase (speB, GAmH_0211). Spermidine may also be synthesized from carboxyspermidine by carboxynorspermidine decarboxylase (nspC, GAmH_0102), which hints at norspermidine production in N. profundicola. Norspermidine plays a significant role in Vibrio cholerae biofilm formation , and a homolog of mbaA (GAmH_1695), a norspermidine-regulated biofilm maintenance protein containing GGDEF and EAL domains in V. cholerae , is present in the N. profundicola genome. However, a homolog of nspS, which encodes a norspermidine-binding regulatory protein in V. cholerae that mediates biofilm formation, was not identified in the N. profundicola genome.
Findings from a recent survey of genes critical for biofilm formation in E. coli reinforced the theory that genes related to surface structures (i.e., flagella and pilin) play major roles in such processes ; similar findings have been reported for C. jejuni –. The highly motile N. profundicola has genes for 56 flagellar and pilin proteins, four chemotaxis proteins, and 11 methyl-accepting chemotaxis proteins. While N. profundicola has most of the same flagellar genes as found in Nitratiruptor sp. SB155-2, the distribution of genes varies between the two organisms. Nitratiruptor flagellar genes are all found within the same region of the genome which has a distinct G+C content , while Nautilia flagellar genes are distributed throughout the genome and are most closely related to flagellar genes in other Epsilonproteobacteria.
Detoxification and Resistance Pathways
There are several genes in N. profundicola and other vent Epsilonproteobacteria that encode proteins directly or peripherally involved in resistance to toxins. One of these is a member of the Nudix family of proteins that clean the cell of toxic nucleotide metabolites . This gene (GAmH_1363) is predicted to encode ADP-ribose pyrophosphatase and is found only in the vent epsiloproteobacterial and Arcobacter butzleri genomes and not in pathogenic Epsilonproteobacteria ,. The protein confers tellurite resistance in both Rhodobacter sphaeroides and Methanococcus jannaschii, presumably through detoxification of ADP-ribose, which non-enzymatically derivatizes terminal amino groups, lysines, and cysteines of proteins –.
N. profundicola has two genes encoding proteins homologous to arsenate reductase genes, one of which (GAmH_1558) is homologous to members of the YffB subfamily of arsenate reductases that reduces arsenate to arsenite via reducing equivalents from glutathione. Similar genes are found in the other three vent Epsilonproteobacteria as well as W. succinogenes and A. butzleri. All the vent Epsilonproteobacteria as well as S. denitrificans and A. butzleri contain homologs to the second arsenate reductase (GAmH_0397 in N. profundicola). W. succinogenes, Sulfurovum, Nitratiruptor, and S. denitrificans contain an apparent operon that encodes an arsenite permease and regulatory protein, ArsR ,,, whereas both N. profundicola and C. mediatlanticus lack an arsenical permease homolog (COG1055), but contain an arsR homolog (GAmH_0374) elsewhere. Therefore, these microbes may not detoxify arsenic or may use an as yet unrecognized mechanism for arsenite transport.
The genome contains two types of cytochromes predicted to be involved in protection against O2 and H2O2: cytochrome bd (cydBA, GAmH_0086, 87), is used to reduce O2 to H2O, and cytochrome c551 peroxidase (GAmH_0241 and 0648) detoxifies peroxide. The genome does not contain predicted genes for catalase or superoxide dismutase, which may be involved in defense against oxidative stress in S. denitrificans . However, N. profundicola has a homolog of alkylhydroperoxide reductase (Ahp, GAmH_0523) that can scavenge endogenous hydrogen peroxide , as well as a predicted peroxide stress regulator (GAmH_0524) which is most closely related to sequences in other Epsilonproteobacteria. The genome also contains 2 homologs of methionine sulfoxide reductases (msrA, GAmH_1068 and msrB, GAmH_0900) predicted to repair oxidative damage.
All bacteria are thought to employ low molecular weight thiols as a component of oxidative stress resistance. These systems are typified by biosynthetic pathways for the thiol (i.e., glutathione or mycothiol), enzymes that utilize the reduced form of thiol as a reductant, and an enzymatic system for reduction of the oxidized disulfide form of the thiol. The N. profundicola genome does not encode a recognizable pathway for biosynthesis of typical redox balancing thiols or a cognate disulfide oxidoreductase. As noted above, N. profundicola contains a potential arsenate reductase that in other organisms requires glutathione as a reductant. Either the N. profundicola version of this enzyme utilizes a different reductant or N. profundicola must synthesize glutathione by a currently unrecognized pathway. Glutathione might be obtained from the environment, but this has only been reported for Streptococcus mutans . Furthermore, we are unaware of any gene encoding a glutathione transporter in any microbial genome.
Coenzyme A (CoA) could serve as an alternative to redox balancing thiol since N. profundicola encodes a potential CoA-disulfide oxidoreductase (GAmH_0923). CoA has been suggested as an alternative to glutathione in the hyperthermophilic archaeon Pyrococcus furiosus, based on high levels of intracellular CoA  and the presence of a CoA-disulfide reductase (CoADR) activity (PF1186) . Recently, however, PF1186 was found to possess NAD(P)H:polysulfide oxidoreductase activity, and the transcript was positively regulated by the addition of elemental sulfur to P. furiosus cultures . This suggests that the CoADR activity could be a side reaction, with a primary function in sulfur metabolism as described below.
N. profundicola was the first chemolithoautotrophic Epsilonproteobacteria isolated from deep-sea hydrothermal vents  and was hypothesized early on to use the rTCA cycle for carbon fixation . Enzymatic evidence, carbon isotopic fractionation patterns, and the genome sequence data presented in this paper all strongly support this hypothesis (Figure 2, cell model) . Like S. denitrificans, N. profundicola encodes two putative fumarate reductase/succinate dehydrogenase (Fdr/Sdh) complexes. One of the complexes (GAmH_1023-1021) is similar to the vent epsilonproteobacterial and S. denitrificans Fdr/sdhABC complexes, the other (GAmH_1096-1094) is more similar to the second copy in C. mediatlanticus as well as those in the Beggiatoa sp. PS (whole-genome amplified and pyrosequenced partial sequence), Aquificales, and Chlorobium genomes ,,. Unlike S. denitrificans, both complexes in the N. profundicola genome contain a putative membrane-anchoring subunit. The genome contains homologs of all of the genes necessary for the generation of 5-carbon sugars via the pentose phosphate pathway as well as the genes necessary for both glycolysis and gluconeogenesis.
Proteins that belong to a given subsystem are color coded as follows: energy metabolism, olive green; carbon (C) metabolism, pale red; nitrogen (N) metabolism, light blue; sulfur (S) metabolism, yellow. Numbers denote the ORFs encoding the enzymes predicted to catalyze a particular reaction. Individual subunits are not drawn to scale. Common energy and reductant carriers shared across subsystems are depicted in green type. Interactions between subsystems based on shared pools of metabolites or reductant carriers are indicated by the dashed blue double arrows. For details on each subsystem, please refer to the appropriate section of the text.
Of the sequenced vent Epsilonproteobacteria, only N. profundicola can grow with formate as a carbon source, but it cannot utilize the C4 dicarboxylic acids (i.e., fumarate or succinate) ,, (Table 1). Therefore, it was surprising to find three homologs to genes encoding C4-dicarboxylate transporter proteins (GAmH_0376, 0470 and 0768) as well as two putative operons with genes encoding a C4-TRAP system for ATP-independent transport of C4 compounds (GAmH_1457-1459, 0113-0115), also found in C. mediatlanticus . The closest homolog to one of the C4-TRAP system operons is from Psychromonas ingrahamii 37, a marine Gammaproteobacterium isolated from polar sea ice . Further experiments are required to determine the functionality of C4 usage in N. profundicola.
Sulfur serves two critical purposes for N. profundicola: as the terminal electron acceptor for energy conservation and as a source of sulfide for biosynthesis. N. profundicola utilizes only elemental sulfur/polysulfides, not sulfate or thiosulfate, as an electron acceptor and presumably must conserve energy in the form of proton motive force from this activity. Three enzymatic systems for the reduction of elemental sulfur have been described: membrane-bound polysulfide reductase , cytoplasmic sulfhydrogenase ,, and a soluble cytoplasmic NAD(P)H:polysulfide oxidoreductase . Membrane-bound polysulfide reductase is exemplified by the Wolinella succinogenes PsrABC enzyme that serves as the terminal oxidase for electron transport from H2 or formate to polysulfide via a quinone. The periplasmic enzyme Sud can accelerate this process by functioning as a polysulfide-binding protein that presents the substrate for reduction by the molybdopterin-containing PsrABC . Phenotypically, N. profundicola should contain this enzyme system ,and homologs of the PsrABC system are indeed encoded by GAmH_1483-1485 (Figures 2 and 3). N. profundicola would presumably use the polysulfides formed from the reaction of S0 and sulfide, which readily occurs at hydrothermal vents and are also found in the culture medium . A homolog of the Sud protein is encoded elsewhere (GAmH_0471). The N. profundicola genome also encodes multiple hydrogenase homologs and one presumptive formate dehydrogenase (GAmH_1274-1276) that forms the input end of an electron transport chain terminating at the PsrABC enzyme.
Enzymes are labeled with the number of the ORF that encodes the protein. The central intermediate in the pathway is periplasmic polysulfide, which is predicted to have two fates. First, polysulfide can be used as the terminal electron acceptor for energy conservation by a quinol oxidizing polysulfide reductase (right branch, 1483–1485). This process likely involves a polysulfide carrier (0471) similar to the Sud protein of Wolinella succinogenes. Second, polysulfide may be assimilated after transport after conversion to sulfide by an NAD(P)H:polysulfide oxidoreductase (center branch, 0923). This is the only route for the synthesis of cysteine and methionine as AmH lacks a reductive sulfate assimilation pathway.
For biosynthesis, all organisms must either obtain sulfur-containing amino acids from the environment or synthesize them de novo by producing and capturing sulfide. In contrast to many bacteria, N. profundicola lacks a sulfate transporter and reductive sulfate assimilation pathway to produce sulfide in the cytoplasm. However, N. profundicola does contain genes that correspond to O-acetyl-serine sulfhydrylase (OAS, GAmH_1543) and O-acetyl-L-homoserine sulfhydrylase (OAHS, GAmH_0859) that capture sulfide for cysteine and methionine biosynthesis, respectively. Based on the genome sequence, we propose that N. profundicola utilizes NAD(P)H polysulfide oxidoreductase (GAmH_0923) to produce sulfide from polysulfide in the cytoplasm for assimilation. GAmH_0923 was noted above as a potential CoA-disulfide oxidoreductase involved in oxidative stress resistance. Clearly, GAmH_0923 is a high priority target for further biochemical and regulation studies to determine whether its primary role in N. profundicola is to serve as a terminal electron acceptor, a mediator of thiol redox balance, or both. A similar lack of reductive sulfate assimilation genes coupled with the presence of sulfide assimilation genes was found in the genome of Thiomicrospira crunogena , a vent-associated microaerobic sulfur-oxidizing chemoautotroph, suggesting that this may be a common trait of organisms inhabiting sulfide rich environments.
N. profundicola grows chemolithotrophically on hydrogen with ammonium or nitrate, but not with urea, as a sole N source . Further growth experiments demonstrated similar specific growth rates (0.059 h−1 vs. 0.044 h−1) on nitrate and ammonium, respectively. However, morphological observations showed elongation and filament production atypical of normal growth in the nitrate-grown cells (data not shown). Genes encoding a typical ammonia transport and assimilation pathway are present: two AmtB transporters (GAmH_0198 and GAmH_0380), NADPH-glutamate synthase (GAmH_1535/ GAmH_1024), and glutamine synthetase (GAmH_0480) (Figures 2 and 4) . As N. profundicola cannot utilize nitrate as a terminal electron acceptor, the presence of genes encoding dissimilatory periplasmic nitrate reductase (napAGHBFD, GAmH_0533-0527) is quite surprising. The nap operon displays high sequence similarity to those encoding periplasmic nitrate reductase in Wolinella succinogenes  and Sulfurimonas denitrificans . Periplasmic nitrate reductase functions in both organisms solely in nitrate respiration and appears to be coupled to nitrite processing systems: in W. succinogenes to the ammonia-forming respiratory pentaheme cytochrome c nitrite reductase, NrfAH , and in S. denitrificans to the NO-forming cytochrome cd1 nitrite reductase, NirS . However, N. profundicola lacks homologs of NrfA, NirS, or any other nitrite reductase, nor are homologs of nitrite transporters (NarK, FocA, etc.) present. Taken together, these results suggest that N. profundicola must utilize a novel pathway for nitrite processing as well as nitrogen assimilation. We propose that N. profundicola utilizes a heretofore unrecognized nitrogen assimilation pathway that relies on the concerted actions of the periplasmic nitrate reductase, a hydroxylamine ubiquinone redox module (HURM, ), and NADH-dependent hydroxylamine reductase (hybrid cluster protein, Har ). The N. profundicola HURM system consists of hydroxylamine oxidoreductase (GAmH_1248) and a cytochrome cM552-like protein (GAmH_0536), while Har is encoded by GAmH_1044.
Enzymes are labeled with the number of the ORF that encodes the protein. The proposed alternative pathway for nitrite reduction to ammonia, predicted to be essential for the growth of AmH on nitrate, is highlighted in orange. The first step of this pathway consists of a hydroxylamine:ubiquinone redox module (HURM) composed of a novel nitrite reductase (1248; reverse hydroxylamine oxidoreductase) that produces hydroxylamine utilizing electrons donated from the (ubi)quinol pool via a cytochrome c protein (0536) in the NapC/NrfH/cM552 protein superfamily. Following transport of hydroxylamine into the cytoplasm, the second step is the reduction of hydroxylamine to ammonia by a hybrid cluster protein/hydroxylamine reductase (1044) utilizing reducing power from a flavin-containing NADH oxidase (0519).
In aerobic and anaerobic ammonia-oxidizing bacteria, HURM functions as a quinone reductase mediating the oxidation of hydroxylamine and hydrazine . In contrast, we propose that the N. profundicola HURM functions in opposite direction and similar to NrfAH as a periplasmic quinol oxidase system that reduces nitrite to hydroxylamine (Figure 4). Recent studies revealed that octaheme cytochrome c hydroxylamine reductase has evolved from pentaheme cytochrome c nitrite reductase, NrfA , via the intermediate octaheme cytochrome c nitrite reductase, ONR ,. If structural features whose evolution have been identified as the crucial event for the conversion of the once N-oxide reductase into an N-oxide dehydrogenase (oxidase) are absent from a given Hao protein sequence, a reverse function of Hao as a nitrite reductase is feasible . Among these structural features missing in the deduced protein sequence of N. profundicola Hao is the critical tyrosine residue (Figure S1), which serves as the protein-derived cross-link to catalytic heme P460 in functional hydroxylamine oxidoreductase functional enzyme complex (HAO) trimers, which is also missing in the NrfA and ONR proteins . Recent work by Pacheco and associates  confirmed that even fully functional hydroxylamine oxidoreductase from Nitrosomonas europaea (a chemolithoautotrophic ammonia oxidizer) can catalyze the reduction of nitric oxide to ammonia given the presence of a proper redox partner. We propose that the cM552-type tetracytochrome c (GAmH_0536) could serve as such suitable redox partner to reverse HAO (GAmH_1248), which needs to be experimentally tested. The uncharged hydroxylamine produced by reverse HAO in the periplasm likely readily diffuses across the plasma membrane, either by simple diffusion or facilitated through ammonia or other major facilitator protein channels. It has been shown that hydroxylamine accumulates readily in the anammoxosome of Kuenenia stuttgartiensis , a structure to be reached by external hydroxylamine only after permeation across the plasma membrane. Uptake studies of hydroxylamine into this anaerobic ammonia-oxidizing planctomycete have also been performed . Once in the cytoplasm, hydroxylamine as a powerful mutagen poses imminent danger and needs to be detoxified as it arrives. This is likely accomplished by NADH-dependent Har, which reduces hydroxylamine to ammonia using electrons served by a flavin-containing NADH oxidoreductase (GAmH_0519). Intriguingly, the promoter region of the har gene contains a conserved recognition motif (CATTGACcgcaGTCAATG) for the FNR-like transcriptional repressors, NnrR and DNR, that are responsive to nitrite and/or nitric oxide . This suggests regulation of at least the har gene by nitrosative intermediates.
The N. profundicola Hao- and cM552-encoding genes are in the vicinity of genes that encode critical functions in the cytochrome c maturation system II of N. profundicola: GAmH_1250, ccdA; GAmH_0538, resB. Also, the hao gene is adjacent to gene encoding a small monoheme cytochrome c (GAmH_1249) homologous to cytochromes c552 (M.G. Klotz, unpublished results), which serve as redox carriers in the respiratory chains of many chemolithotrophs and are known to redox-partner with complexes III and IV ,,. The N. profundicola genome does not encode a complex III, and complex IV is a cytochrome D ubiquinol oxidase (GAmH_0086-89). Because the predicted catabolic modules in the periplasm interact directly with the quinone pool (Psr, FDH, uptake-hydrogenase), cytochrome c552 likely connects both or one of the encoded cytochrome c551 peroxidases (GAmH_0241, GAmH_0648) to cM552, thereby providing hydrogen peroxide tolerance and an additional respiratory electron sink to the quinone pool. A putative redox interaction between cytochromes c552 and cM552 has been discussed in the literature ( and references therein).
We have preliminary evidence to suggest that at least three genes in the pathway, napA, hao, and har, are upregulated in N. profundicola cells grown in the presence of nitrate vs. ammonium. These experiments are ongoing and will be published at a later date. This pathway may also be present in other bacteria, at least in other Nautilia strains. The genome sequence of Nautilia lithotrophica is currently being sequenced, and other Nautilia strains are also being tested for growth on nitrate as an N source.
N. profundicola has a number of conserved genes involved in energy metabolism. We concentrate here on those that are unique, either to N. profundicola or other hydrothermal vent microbes. The importance of H2 metabolism observed in the physiology of N. profundicola is clearly reflected by the number of operons for hydrogenases. As evidenced by the phylogenetic relationships of the large subunits, there are one H2-sensing (Group 2), two H2-uptake (Group 1), and three H2-evolving (Group 4) hydrogenases in the N. profundicola genome (Figure 5). One of the membrane uptake (GAmH_0551-0556) and the sensing (GAmH_0548, 0549) hydrogenase operons are similar to those in other vent Epsilonproteobacteria (Nitratiruptor sp., Sulfurovum, C. mediatlanticus). The second uptake hydrogenase (NiFeSe, GAmH_0978, GAmH_0979) is most similar to that from Desulfotalea psychrophila LSv54 (DP0159, DP0160), Deltaproteobacterium MLMS-1, and other members of the Deltaproteobacteria, and the gene product is likely soluble and periplasmic because there is no membrane anchor subunit. It is induced in Desulfovibrio vulgaris str. Hildenborough under H2- and Se-replete conditions . There are three predicted operons in the N. profundicola genome for H2-evolving hydrogenases (Group 4): ech, hyc, and coo. The ech (GAmH_0607-0612) and hyc (GAmH_0723-0718) operons are identical in gene order to those in other described genomes (e.g., ech, Desulfovibrio vulgaris and Methanosarcina barkeri; hyc, Rhodopseudomonas palustris and, Methanosarcina mazei) with two internal genes conserved in the hyc operon not found in the E. coli hyc operon ,,,. The ech genes are most similar to that of C. mediatlanticus. Although both N. profundicola and C. mediatlanticus have the large and small subunits of CO-induced hydrogenase (CooLH), only N. profundicola contains the full operon structure (cooMKLXUHF3, GAmH_1032-1026), similar to both Desulfovibrio vulgaris and Carboxydothermus hydrogenoformans, (Figure S2) ,.
Distance topologies were performed in MEGA4, based on the Neighbor-joining method after alignment with ClustalW (BLOSUM matrix). Bootstrap values (500 replicates) are indicated on branches. Indicated groupings are based on previous analyses.
The physiological roles of the multiple hydrogenases likely reflect an adaptation to variable hydrogen and electron acceptor concentrations, though defining their specific roles will require significant experimental effort. We propose that the Group 1 membrane bound hydrogenase is a constitutive system that couples to the quinone pool and contributes to establishing and maintaining the proton gradient across the cytoplasmic membrane. Three of the hydrogenases appear to couple with ferredoxin. The reduction of ferredoxin with molecular hydrogen is thermodynamically unfavorable due to the difference in redox potentials with the reaction becoming even more unfavorable at low hydrogen concentrations. Two of the Group 4 energy-conserving hydrogenases may be able to utilize proton or sodium motive force to overcome this energy requirement and may be utilized to help provide reduced ferredoxin for CO2 fixation when hydrogen concentrations are low. This has been proposed for the homologous Methanosarcina barkeri Ech hydrogenase during autotrophic growth . The soluble, cytoplasmic Group 2 hydrogenase cannot couple to ionic gradients, and we therefore hypothesize that it may only be important when hydrogen concentrations in the environment are high and the cell rapidly oxidizes reduced ferredoxin, conditions that would minimize the unfavorable energetics. This scenario has been proposed for the Aquifex aeolicus cytoplasmic hydrogenase, the closest characterized homolog to that of N. profundicola ,. Alternatively, this enzyme could be important during hydrogen and polysulfide starvation. Under these conditions, strain AmH may degrade internal carbon stores by running the rTCA cycle oxidatively, and the hydrogenase would reoxidize reduced ferredoxin, allowing the cycle to continue and produce hydrogen in a favorable reaction. The aforementioned Group 4 hydrogenases could also contribute in this scenario by maintaining a proton or sodium gradient. The role of the third Group 4 hydrogenase, CooLH, is enigmatic as N. profundicola does not, nor do any of the other Epsilonproteobacteria, encode a CooS-type CODH subunit that is required for CO-dependent H2 evolution in Rhodospirillum rubrum . Similarly, the role of the periplasmic Group 1 hydrogenase is unclear.
Besides the hydrogenases and a typical F1F0 ATPase enzyme complex (F0, GAmH_1440; F1, GAmH_0301-0306) closely related to other Epsilonproteobacteria ATPases, a vacuolar-type H+-transporting ATPase operon (GAmH_0030-0023) was also found in the N. profundicola genome. The genes in the operon are most closely related to Halothermothrix orenii H168 and Clostridium tetani E88, and other members of the Clostridia (Firmicutes) ,. Similar operon structures are found in Clostridium tetani and Nitrosococcus oceani and differ somewhat from the archaeal operons (e.g., Methanosarcina mazei, Pyrococcus horikoshi, and Thermococcus kodakaraensis KOD1) ,,,. Since this operon is only in the N. profundicola and not in any other epsilonproteobacterial genome, it was likely acquired via a horizontal gene transfer event. The function of this complex may be partially similar to that of N. oceani and other AOB, where it actively extrudes sodium . However, N. profundicola lacks sodium-dependent complex I that in N. oceani likely reduces NAD to NADH . N. profundicola contains several types of homologous genes that code for sodium transport proteins: sodium-proton antiporters (GAmH_0108, GAmH_1552, GAmH_0762, GAmH_0955) most likely used for motility and transport processes; a putative sodium-proline symporter (GAmH_0965, PutP) for amino acid uptake; and a putative sodium di- and tricarboxylate transporter (GAmH_0459, TrkA-C). N. profundicola likely uses the V-type ATPase to regulate the influx of sodium ions from these processes.
The N. profundicola genome contains all but 4 (nuoE, F, G, K) of the subunits for NADH:ubiquinone oxidoreductase (NADH dehydrogenase, complex I), which in other species contributes to proton motive force generation for oxidative phosphorylation. The gene structure here is identical to some other anaerobic bacteria, including C. mediatlanticus and Carboxydothermus hydrogenoformans, but is missing two genes (F and G) that normally form the inner cytoplasmic structure of the oxidoreductase found in facultative anaerobes and microaerophilic bacteria belonging to the Epsilonproteobacteria. The missing part makes up the acceptor region of the molecule, and therefore these anaerobes most likely have a different acceptor portion of the complex not found in the nuo operon. In addition to the fumarate reductase mentioned above, the genome also contains a four-subunit formate dehydrogenase (GAmH_1274-1277), which enables N. profundicola to grow with formate as an electron donor and carbon source .
Cell energy requirements include both the reduced ferredoxin to drive rTCA cycle CO2-fixing reactions and reduced pyridine nucleotides to drive other anabolic processes. The production of reduced ferredoxin from hydrogen was discussed above. As N. profundicola lacks the genes necessary to generate NAD(P)H via the Pentose Phosphate pathway or via a ferredoxin-linked NADH oxidoreductase, we hypothesize that the partial complex I (NUO) acting in concert with formate dehydrogenase (FDH) or the membrane-bound uptake hydrogenase could form a reverse electron transport system responsible for producing NAD(P)H (Figure 6). Additional proton motive force to drive the system could be supplied by the V-type ATPase. In addition to hydrogen and formate, the polysulfide reductase might be able to act in the reverse direction and oxidize sulfide to polysulfide, or succinate could be oxidized to fumarate via a succinate dehydrogenase. Both of these activities would also reduce the quinone pool to provide electrons under low hydrogen/high sulfide, and/or inorganic electron donor-limited conditions. This flexibility in electron donor usage may be an adaptation of N. profundicola to the highly variable hydrothermal vent environment.
The ultimate reduction of NAD(P)+ from the quinone pool is endergonic and requires energy in the form of coupling to the proton motive force, which may be provided, in part, by a V-type ATPase. Numbers on complexes refer to the ORF numbers of the encoding genes.
DNA Replication and Repair
Several DNA replication genes found in all vent Epsilonproteobacteria (but not exclusive to this group) include: nucleotidyltransferase/DNA polymerase (GAmH_0589); DNA polymerase (Pol) III epsilon subunit and related 3′–5′ exonucleases (GAmH_0148, GAmH_0193); and the gram positive type of DNA Pol III alpha subunit (GAmH_0988, GAmH_1246), which contains an internal proofreading exonuclease . Only four Epsilonproteobacteria, including N. profundicola and C. mediatlanticus, contain multiple homologs of the Gram-positive DNA Pol III alpha subunit, and most of the vent Epsilonproteobacteria and a few free-living members contain 2–3 homologs of the epsilon subunit. The epsilon subunit provides proofreading for DNA Pol III (mutD in E. coli), and binds to the polymerase and histidinol phosphatase (php) domain of the E. coli DNA Pol III alpha subunit . Wieczorek et al. (2006) suggested that the similar php domain found in DNA PolX, mentioned below, might also bind the epsilon subunit, enabling an additional coupled proofreading reaction beyond the DNA Pol III enzyme complex. The inventory of epsilon and alpha DNA Pol III subunits and DNA Pol X in vent Epsilonproteobacteria supports this hypothesis.
There are three genes whose functions relate to DNA replication and repair and are found exclusively in the vent Epsilonproteobacteria and not in the subdivision as a whole; they may be very important in the repair of damaged DNA required under the extreme conditions found at vents (Table 1). First, a homolog to the DNA-repair photolyase or spore photoproduct lyase gene (GAmH_0507) is present. It is a member of the radical S-adenosylmethionine (SAM, GAmH_0452 in N. profundicola) domain protein superfamily (Figure S3). This gene shares a strong similarity to other members of this superfamily cluster found in three out of four of the vent Epsilonproteobacteria (data not shown) but is not found in non-vent Epsilonproteobacteria. The most similar experimentally characterized protein to the gene in N. profundicola is a gene from Bacillus subtilis (23% protein sequence identity) which functions to repair thymine dimers (5-thyminyl-5,6-dihydrothymine, or spore product) specifically formed in spores during UV exposure . Although there is evidence of some light emissions from deep-sea hydrothermal vents, most likely from geothermal energy found above 700 nm , there is no evidence of UV emissions. A member of this family was also identified in the genome of Idiomarina loihiensis L2TR, a Gammaproteobacterium isolated from a deep-sea hydrothermal vent at Loihi, Hawaii ,. In these vent bacteria, the related gene product could function in repair of damage to DNA generated by other processes. Possible conditions that could lead to nucleotide damage include high levels of ionizing radiation and metal complexes with reactive oxidative species , –109. While there are few direct measurements of ionizing radiation on chimney surfaces, researchers have isolated bacteria and archaea resistant to high levels of radiation (>20–30 kGy) and found polychaetes containing very high levels of natural Pb-210 and Po-210 –.
The second gene predicted to be involved in DNA replication and repair found in all vent Epsilonproteobacteria is DNA polymerase IV, family X (PolX, GAmH_1417). PolX homologs are found in 25–30% of sequenced bacterial genomes, and genetic evidence indicates that PolX contributes to double strand break repair in the radiation-resistant bacterium Deinococcus radiodurans . Eukaryotic PolX homologs act to repair double strand breaks with 3′-overhangs containing gaps on both strands, i.e., non-homologous end repair. While experimental and bioinformatics evidence suggest that a Ku-LigD system for non-homologous end repair is present in a wide variety of bacterial groups, it does not appear to be present in Epsilonproteobacteria ,. Therefore, PolX in Epsilonproteobacteria may substitute for the Ku-LigD system in countering DNA damage.
Finally, a gene involved in DNA stability at high temperatures was found exclusively in vent associated Epsilonproteobacteria: reverse gyrase (rgy, GAmH_1041, Figure 7). Rgy, long considered a hallmark protein of hyperthermophiles, likely acts as a DNA chaperone by preferentially binding to nicked DNA during repair . Reverse gyrase is named for its ATP-dependent introduction of positive supercoiling, which protects against DNA damage at high temperatures and may also introduce negative supercoiling –. Reverse gyrase has previously been reported only in microbes whose optimal growth temperatures are above 65°C, over 20°C above that of N. profundicola . Although considered a hallmark protein of hyperthermophiles, Rgy may not be essential for growth at high temperatures. A rgy mutant of Thermococcus kodakaraensis KOD1 grew at high temperatures, albeit more slowly than the wild type, with a corresponding decrease in the upper growth limit .
Distance topologies were performed in MEGA4, based on the Neighbor-joining method after alignment with ClustalW (BLOSUM matrix). Bootstrap values (500 replicates) are indicated on branches. Nearly identical topologies were observed with Maximum Likelihood (phyml), Minimum Evolution and Maximum Parsimony algorithms (data not shown).
We report here that other deeply-branching hydrothermal vent Epsilonproteobacteria, Caminibacter mediatlanticus  and Nitratiruptor sp. SB155-2 , also contain a highly related rgy sequence; their optimal growth temperatures are 55°C (Table 1, Figure 7). Brochier-Armanet and Forterre (2006) argued for an ancient bacterial acquisition of rgy from Archaea. However, the analysis presented here indicates a clear separation between the bacterial and archaeal clades, which suggests descent from a common ancestor. Thermotoga and Clostridia rgy sequences do form a separate clade closer to the archaeal clade than other bacterial groups (Figure 7), perhaps suggesting a lateral transfer in some groups. The rgy gene is not found in the genome of one sequenced hydrothermal vent member of the Epsilonproteobacteria, Sulfurovum sp. NBC37-1 . This strain, which is mesophilic (Topt = 33°C), was isolated from an in situ sampler and is phenotypically more similar to Thiomicrospira crunogena, a vent- associated microaerobic sulfur-oxidizing chemoautotroph that also does not contain the rgy gene ,.
A PCR survey of thermophilic Nautiliales from geographically distinct sites indicated that an rgy gene is also present in Lebetimonas acidiphila (Topt = 50°C)  and Nautilia sp. strain 4064-55 (Topt = 55°C). These sequences formed a monophyletic clade with N. profundicola and C. mediatlanticus rgy sequences (Figure 8). However, Nautilia lithotrophica, isolated from the same geographic location as N. profundicola , returned negative results in this survey. These results suggest that if the Epsilonproteobacteria acquired rgy by horizontal gene transfer, it would have been prior to their split from other bacterial groups or prior to the split of the Nautiliales order, whereby some could have lost this gene or the gene sequence diverged enough to be undetectable with the degenerate primers used in the study.
Distance topologies were performed in MEGA4, based on the Neighbor-joining method after alignment with ClustalW (BLOSUM matrix). Bootstrap values (500 replicates) are indicated on branches.
To determine if rgy might be involved in stress response or DNA repair, expression of this gene in cultured cells was monitored by quantitative reverse transcription-PCR (qRT-PCR). Transcript abundance of N. profundicola rgy increased over 100 fold when actively growing cells were exposed to 65°C while rRNA transcript abundance remained constant or slightly decreased (Figure 9). The same response was not observed at 35, 45, or 55°C. These results indicate that N. profundicola rgy transcription is induced by thermal stress and, by extension, suggest that the N. profundicola rgy gene product is involved in at least thermal stress response. Our results agree with previous data suggesting that Rgy activity is increased in hyperthermophiles at higher temperatures . The upregulation of rgy at higher temperatures, combined with previous data that suggest that Rgy is not absolutely required for growth at high temperatures , may imply that Rgy is involved in other functions, such as gene regulation or promoter activity.
Values were calculated per ng of RNA. Standard errors indicated by the error bars. Y axes are on a log scale.
To further extend these observations, the occurrence, phylogeny, and expression of rgy in vivo was assessed in representative deep-sea hydrothermal vent samples from 9°N and Guaymas Basin along the East Pacific Rise. PCR with degenerate primers produced a number of rgy sequences from these samples, including two groups (referred to as Epsilon-like) that are distinct from rgy sequences present in cultured members of the Epsilonproteobacteria (Figures 8, 10). These Epsilon-like rgy sequences were the most frequently observed in the vent survey with one exception. Primers specific for the geographically distinct members of the Epsilon-like groups were used in qPCR experiments on samples from varying temperatures. These experiments indicated that the Epsilon-like groups were more abundant in samples from chimneys with lower temperatures (8–20°C), where as much as 27% of the bacterial population, when normalized to 16S rRNA gene abundance, appeared to contain an Epsilon-like rgy gene (Table 3). Perhaps organisms containing the Epsilon-like rgy gene may normally experience low temperatures, but display enhanced resistance to short term temperature spikes. In support of this theory, RT-PCR with specific Epsilon-like rgy primers demonstrated that this gene is transcribed in situ (data not shown).
9N-773 (Io vent); 9N-POB (P vent, bottom); 9N-243T (Alvinella tube scraping); G-Rebecca's Roost (Guaymas Basin flange); G-855 (Guaymas Basin flange). Groups were reported as clades with nearest cultured representatives because of poor bootstrap values on the distal branch points, most likely due to the length of the aligned sequence.
The Epsilonproteobacteria, while perhaps better recognized as pathogens, are supremely adapted to hydrothermal vent ecosystems . The N. profundicola genome reported here indicates that this organism's success in the vent environment relies on chemoautotrophy via the rTCA cycle and a tendency to reduce sulfur species driven by a prodigious capacity for hydrogen oxidation. It is truly remarkable that N. profundicola lacks much of the well-established electron transport chain inventory, but is able to utilize its large suite of hydrogenases and ATPases, the most described yet in any Epsilonproteobacteria, for energy and reducing equivalents. Presumably, these genes and pathways would be tightly regulated to follow the prevailing redox conditions found at highly fluctuating diffuse flow vents. The abundance of signal transduction genes similar to other free-living vent Epsilonproteobacteria implies that the lifestyle of N. profundicola is mainly independent of A. pompejana and its episymbionts. The N. profundicola genome also produced two unique insights that will drive further investigations. First, we propose a novel pathway for nitrate assimilation where Nap, HURM (Hao and cM552), and Har function together to produce ammonium via the toxic intermediate hydroxylamine.
Second, N. profundicola, along with C. mediatlanticus and Nitratiruptor sp., contains a gene homologous to reverse gyrase, which most likely confers a selective advantage to these Epsilonproteobacteria in the face of rapid temperature fluctuations that occur at deep-sea hydrothermal vents. N. profundicola, therefore, contains all the genes necessary for life in conditions widely believed to reflect those in the archaean biosphere: anaerobic, sulfur, H2- and CO2-rich, with unstable redox potentials and temperatures ,. N. profundicola will thus be an important model system to understand early microbial life on earth.
Material and Methods
Growth and Sequencing of N. profundicola
N. profundicola (Nautilia sp. AmH) was grown in an anaerobic autotrophic sulfur medium as described . DNA was extracted from the cultured cells using a standard SDS/proteinase K/phenol/chloroform extraction technique . The genome was sequenced with the whole-genome shotgun method with both small and medium insert libraries as previously described . Physical and sequencing gaps were closed as described . Assembly was performed with the Celera Assembler version 3. An initial set of predicted ORFs and the functional assignment of genes was performed using the TIGR (now JCVI) autoannotation pipeline. This included gene finding with Glimmer, Blast-extend-repraze (BER) searches, HMM searches, TMHMM searches, SignalP predictions, and automatic annotations from AutoAnnotate.
For nitrate growth analysis, N. profundicola was cultivated in anaerobic salt water media with S0 and a headspace of H2/CO2 gas as previously described , with either 5 mM ammonium or 20 mM nitrate as the sole nitrogen source. After three passes in the indicated media, cell growth was monitored by direct counts of cells stained with 4′, 6-diamidino-2-phenylindole using an Olympus Provis AX70 microscope and image analysis software (ImagePro Plus, Media Cybernetics). The production of hydrogen sulfide was monitored during cell growth using the Cline method .
The manual annotation tool Manatee was used to manually review the output from the prokaryotic pipeline of the TIGR (now JCVI) annotation pipeline (http://manatee.sourceforge.net/). Manual curation was performed on approximately 383 CDS. Further refinements and comparisons to other genomes were done with IMG, BLAST, and RAST –. GenBank accession number of the genome is CP001279. The N. profundicola genome report as recommended by Genome Standards Consortium  is outlined (Table S1).
To identify redundant/duplicated operons, an all-vs-all self blastp was performed and an E-value cutoff (of 10–20) was applied to the results to establish links between proteins. The results were then visually analyzed using DagChainer , and no evidence of duplicated operons was found. Potential prophage regions were located by manually searching genome regions near tRNA as well as with the automated online tool, Prophage Finder , which looks for phage-like genes and changes in G+C content.
Phylogenetic trees of the translated rgy and large subunit hydrogenase sequences were constructed in MEGA4  with the default parameters, based on both Neighbor-joining and minimum evolutionary algorithms with 500 bootstrap replicates. PHYML trees were calculated with the following parameters: Bootstrap replica number: 100; Substitution model: JTT; Proportion of invariable sites: automatic estimated by the program; Number of substitution rate categories: 4; Gamma distribution parameter: automatic estimated by the program; and Starting tree: BIONJ. The program was allowed to optimize the topology, the branch lengths and rate parameters of the start tree.
Abundance of N. profundicola rgy mRNA
Cells were grown at 45°C to mid-log phase (∼12 hours) and triplicate tubes shifted to each indicated temperature for 2 hours. RNA was harvested using a CTAB extraction protocol , and genomic DNA was removed by gDNA wipeout buffer (Qiagen QuantiTect Reverse Transcription Kit). RNA samples were quantified using the RiboGreen Assay (Invitrogen) and diluted to the same concentrations with RNAase-free H2O. cDNA was produced using random primers and the Qiagen QuantiTect Reverse Transcription Kit.
To estimate the abundance of rgy mRNA, specific primers were designed from the nucleotide sequences of the N. profundicola rgy gene (AmHrgy2022F: 5′-CACAGACCCCGA TAGGGAAGGTG; AmHrgy2203R: 5′-ACCCGACCCATCTGTCCGTAATTC). In addition, the 16S rRNA was quantified using primers and conditions described elsewhere ,. Genomic DNA was quantified using the picogreen assay (Invitrogen) and used in standard curves for both reactions. Quantitative PCR was performed in triplicate or quadruplicate with 1 µl of cDNA in a final volume of 12.5 µl using the Stratagene SYBR green mix on an ABI7500, and PCR conditions for the rgy primer set were: 95°C 10 minutes; followed by 40 cycles of amplification at 95°C for 15 seconds, 62°C for 30 seconds and 72°C for 45 seconds, with a final dissociation step. Final rgy primer concentrations were at 0.05 µM.
Environmental Sample Analyses
Chimney and Alvinella pompejana tube samples were collected as described previously during four cruises to the EPR in November 1999 (9°N, Io vent -773), January 2000 (Guaymas Basin, 534G and 855G), the Extreme cruises Extreme 2001, November 2001 (9°N, P vent-POB) and Extreme 2003, November 2003 (Alvinella pompejana tube-243T). In addition, a chimney sample (Michael's vent, Extreme 2004 cruise, Nov.-Dec., 2004, 9°N, EPR) was inoculated into anaerobic autotrophic sulfur medium as described  and incubated at 55°C. One strain (Nautilia sp. strain 4064-55) was detected in the third subculture by DGGE and sequenced as described . DNA and RNA were extracted from the indicated samples and RNA was reverse transcribed as described previously with a modified rgy reverse PCR primer, without the KpnI restriction site ,.
Reverse Gyrase PCR Amplifications, Cloning, and Abundance Estimates
A portion of the rgy gene from environmental samples was PCR amplified with degenerate primers as previously described , cloned into a TOPO TA vector, and sequenced . GenBank accession numbers of the clones are FJ597068-FJ597141. Two rgy clades (Epsilon-like 1 and Epsilon-like 2) were chosen for quantitative estimates based on SYBR green quantification on an ABI7500. Primers were: Egrp1 rgyF (5′-TGGATCGGTTTCGGAATTTCCG); Egrp1 rgyR (5′-GCAAATGCGTTCTCGAAACGTTC); Egrp2 rgyF (5′-GGTGGATCGGATTCGGGATTTC); and Egrp2 rgyR (5′-CTTGAATGCGAACTCGAGGCG). Conditions and primer concentrations were as described for RT-qPCR above, but with a two-step PCR reaction (95°C for 15 seconds, 65°C for 2 minutes). Standard curves were generated from dilutions of cloned DNAs within the specific clade (Epsilon-like 1 or 2).
Alignment of the C-termini of Hao proteins with annotations based on Igarashi et al.  and Bergmann et al. : CxxCH, heme binding motif 8; H459, axial ligand to heme; Y467, protein crosslink to catalytic heme; major alpha helices; alpha TMS, C-terminal transmembrane spanning domain. Sequence sources: CAD84873, Nitrosomonas europaea ATCC 19718; ABB75723, Nitrosospira multiformis ATCC 25196; ABA57404, Nitrosococcus oceani ATCC 19707; AAU92745, Methylococcus capsulatus Bath; EDM23995, Caminibacter mediatlanticus TB-2; AM-H, Nautilia profundicola AM-H. , .
(4.15 MB TIF)
Gene neighborhood of rgy gene (Reverse gyrase, GAMH_1041), coo (CO-hydrogenase, GAMH_1032-1026) and fdr (Fumarate reductase, GAMH_1024-1022) operons in Nautilia profundicola (A) and Caminibacter mediatlanticus (B). Genes of the same color (except light yellow) are from the same orthologous group (top COG hit).
(2.94 MB TIF)
Gene neighborhoods of the spl-related gene (DNA photolyase, Radical SAM domain protein) in Nautilia profundicola (A) and Caminibacter mediatlanticus (B). Genes of the same color (except light yellow) are from the same orthologous group (top COG hit). Abbreviations: spl = DNA photolyase; H = hypothetical protein; CH = conserved hypothetical; 1 = GAMH_0510, xanthine guanine phosphoribosyl transferase; 2 = GAMH_0506, short chain dehydrogenase; 3 = GAMH_0503, plasmid stabilization system; 4/5 = GAMH_0504/502, toxin/antitoxin gene pair, RelBE-3 family. Arrows indicate a probable genome inversion between the strains.
(7.78 MB TIF)
The authors would like to thank the Captain and Crew of the R/V l'Atalante and especially the Nautile pilots for help with sample collection.
Conceived and designed the experiments: BJC JAE SCC. Performed the experiments: BJC JLS JMR HMK. Analyzed the data: BJC JLS TEH MGK LYS CKL DW JAE. Contributed reagents/materials/analysis tools: JAE. Wrote the paper: BJC JLS TEH MGK CKL.
- 1. Lowell RP, Rona PA, Vonherzen RP (1995) Sea-floor hydrothermal systems. J Geophys Res-Sol Ea 100: 327–352.RP LowellPA RonaRP Vonherzen1995Sea-floor hydrothermal systems.J Geophys Res-Sol Ea100327352
- 2. Luther GW, Rozan TF, Taillefert M, Nuzzio DB, Di Meo C, et al. (2001) Chemical speciation drives hydrothermal vent ecology. Nature 410: 813–816.GW LutherTF RozanM. TaillefertDB NuzzioC. Di Meo2001Chemical speciation drives hydrothermal vent ecology.Nature410813816
- 3. Von Damm KL (1995) Controls of the chemistry and temporal variability of seafloor hydrothermal fluids. In: Humphris SE, Zierenberg RA, Mullineaux LS, Thomson RE, editors. Seafloor hydrothermal systems: Physical, chemical, biological, and geological interactions. Washington, D.C.: American Geophysical Union. pp. 222–247.KL Von Damm1995Controls of the chemistry and temporal variability of seafloor hydrothermal fluids.SE HumphrisRA ZierenbergLS MullineauxRE ThomsonSeafloor hydrothermal systems: Physical, chemical, biological, and geological interactionsWashington, D.C.American Geophysical Union222247
- 4. Scheirer DS, Shank TM, Fornari DJ (2006) Temperature variations at diffuse and focused flow hydrothermal vent sites along the northern East Pacific Rise. Geochem Geoph Geosy 7: Q03002.DS ScheirerTM ShankDJ Fornari2006Temperature variations at diffuse and focused flow hydrothermal vent sites along the northern East Pacific Rise.Geochem Geoph Geosy7Q03002
- 5. Kadko D (1996) Radioisotopic studies of submarine hydrothermal vents. Rev Geophys 34: 349–366.D. Kadko1996Radioisotopic studies of submarine hydrothermal vents.Rev Geophys34349366
- 6. La Duc MT, Benardini JN, Kempf MJ, Newcombe DA, Lubarsky M, et al. (2007) Microbial diversity of Indian Ocean hydrothermal vent plumes: Microbes tolerant of desiccation, peroxide exposure, and ultraviolet and gamma-irradiation. Astrobiology 7: 416–431.MT La DucJN BenardiniMJ KempfDA NewcombeM. Lubarsky2007Microbial diversity of Indian Ocean hydrothermal vent plumes: Microbes tolerant of desiccation, peroxide exposure, and ultraviolet and gamma-irradiation.Astrobiology7416431
- 7. Jolivet E, L'Haridon S, Corre E, Forterre P, Prieur D (2003) Thermococcus gammatolerans sp nov., a hyperthermophilic archaeon from a deep-sea hydrothermal vent that resists ionizing radiation. Int J Syst Evol Micr 53: 847–851.E. JolivetS. L'HaridonE. CorreP. ForterreD. Prieur2003Thermococcus gammatolerans sp nov., a hyperthermophilic archaeon from a deep-sea hydrothermal vent that resists ionizing radiation.Int J Syst Evol Micr53847851
- 8. Cherry R, Desbruyeres D, Heyraud M, Nolan C (1992) High levels of natural radioactivity in hydrothermal vent polychaetes. CR Acad Sci III 315: 21–26.R. CherryD. DesbruyeresM. HeyraudC. Nolan1992High levels of natural radioactivity in hydrothermal vent polychaetes.CR Acad Sci III3152126
- 9. Miroshnichenko ML (2004) Thermophilic microbial communities of deep-sea hydrothermal vents. Microbiology 73: 1–13.ML Miroshnichenko2004Thermophilic microbial communities of deep-sea hydrothermal vents.Microbiology73113
- 10. Reysenbach AL, Cady SL (2001) Microbiology of ancient and modern hydrothermal systems. Trends Microbiol 9: 79–86.AL ReysenbachSL Cady2001Microbiology of ancient and modern hydrothermal systems.Trends Microbiol97986
- 11. Campbell BJ, Engel AS, Porter ML, Takai K (2006) The versatile epsilon-proteobacteria: key players in sulphidic habitats. Nat Rev Microbiol 4: 458–468.BJ CampbellAS EngelML PorterK. Takai2006The versatile epsilon-proteobacteria: key players in sulphidic habitats.Nat Rev Microbiol4458468
- 12. Huber JA, Welch DBM, Morrison HG, Huse SM, Neal PR, et al. (2007) Microbial population structures in the deep marine biosphere. Science 318: 97–100.JA HuberDBM WelchHG MorrisonSM HusePR Neal2007Microbial population structures in the deep marine biosphere.Science31897100
- 13. Huber JA, Butterfield DA, Baross JA (2003) Bacterial diversity in a subseafloor habitat following a deep-sea volcanic eruption. FEMS Microbiol Ecol 43: 393–409.JA HuberDA ButterfieldJA Baross2003Bacterial diversity in a subseafloor habitat following a deep-sea volcanic eruption.FEMS Microbiol Ecol43393409
- 14. Takai K, Inagaki F, Nakagawa S, Hirayama H, Nunoura T, et al. (2003) Isolation and phylogenetic diversity of members of previously uncultivated epsilon-proteobacteria in deep-sea hydrothermal fields. FEMS Microbiol Lett 218: 167–174.K. TakaiF. InagakiS. NakagawaH. HirayamaT. Nunoura2003Isolation and phylogenetic diversity of members of previously uncultivated epsilon-proteobacteria in deep-sea hydrothermal fields.FEMS Microbiol Lett218167174
- 15. Reysenbach AL, Longnecker K, Kirshtein J (2000) Novel bacterial and archaeal lineages from an in situ growth chamber deployed at a Mid-Atlantic Ridge hydrothermal vent. Appl Environ Microb 66: 3798–3806.AL ReysenbachK. LongneckerJ. Kirshtein2000Novel bacterial and archaeal lineages from an in situ growth chamber deployed at a Mid-Atlantic Ridge hydrothermal vent.Appl Environ Microb6637983806
- 16. Longnecker K, Reysenbach AL (2001) Expansion of the geographic distribution of a novel lineage of epsilon-Proteobacteria to a hydrothermal vent site on the Southern East Pacific Rise. FEMS Microbiol Ecol 35: 287–293.K. LongneckerAL Reysenbach2001Expansion of the geographic distribution of a novel lineage of epsilon-Proteobacteria to a hydrothermal vent site on the Southern East Pacific Rise.FEMS Microbiol Ecol35287293
- 17. Corre E, Reysenbach AL, Prieur D (2001) Epsilon-proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. FEMS Microbiol Lett 205: 329–335.E. CorreAL ReysenbachD. Prieur2001Epsilon-proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge.FEMS Microbiol Lett205329335
- 18. Nakagawa S, Takaki Y, Shimamura S, Reysenbach AL, Takai K, et al. (2007) Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens. P Natl Acad Sci USA 104: 12146–12150.S. NakagawaY. TakakiS. ShimamuraAL ReysenbachK. Takai2007Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens.P Natl Acad Sci USA1041214612150
- 19. Voordeckers JW, Starovoytov V, Vetriani C (2005) Caminibacter mediatlanticus sp nov., a thermophilic, chemolithoautotrophic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. Int J Syst Evol Micr 55: 773–779.JW VoordeckersV. StarovoytovC. Vetriani2005Caminibacter mediatlanticus sp nov., a thermophilic, chemolithoautotrophic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge.Int J Syst Evol Micr55773779
- 20. Campbell BJ, Jeanthon C, Kostka JE, Luther GW, Cary SC (2001) Growth and phylogenetic properties of novel bacteria belonging to the epsilon subdivision of the Proteobacteria enriched from Alvinella pompejana and deep-sea hydrothermal vents. Appl Environ Microb 67: 4566–4572.BJ CampbellC. JeanthonJE KostkaGW LutherSC Cary2001Growth and phylogenetic properties of novel bacteria belonging to the epsilon subdivision of the Proteobacteria enriched from Alvinella pompejana and deep-sea hydrothermal vents.Appl Environ Microb6745664572
- 21. Smith JL, Campbell BJ, Hanson TE, Zhang CL, Cary SC (2008) Nautilia profundicola sp. nov., a thermophilic, sulfur-reducing epsilonproteobacterium from deep-sea hydrothermal vents. Int J Syst Evol Micr 58: 1598–1602.JL SmithBJ CampbellTE HansonCL ZhangSC Cary2008Nautilia profundicola sp. nov., a thermophilic, sulfur-reducing epsilonproteobacterium from deep-sea hydrothermal vents.Int J Syst Evol Micr5815981602
- 22. Alain K, Querellou J, Lesongeur F, Pignet P, Crassous P, et al. (2002) Caminibacter hydrogeniphilus gen. nov., sp nov., a novel thermophilic, hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent. Int J Syst Evol Micr 52: 1317–1323.K. AlainJ. QuerellouF. LesongeurP. PignetP. Crassous2002Caminibacter hydrogeniphilus gen. nov., sp nov., a novel thermophilic, hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent.Int J Syst Evol Micr5213171323
- 23. Miroshnichenko ML, Kostrikina NA, L'Haridon S, Jeanthon C, Hippe H, et al. (2002) Nautilia lithotrophica gen. nov., sp nov., a thermophilic sulfur-reducing epsilon-proteobacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Micr 52: 1299–1304.ML MiroshnichenkoNA KostrikinaS. L'HaridonC. JeanthonH. Hippe2002Nautilia lithotrophica gen. nov., sp nov., a thermophilic sulfur-reducing epsilon-proteobacterium isolated from a deep-sea hydrothermal vent.Int J Syst Evol Micr5212991304
- 24. Miroshnichenko ML, L'Haridon S, Schumann P, Spring S, Bonch-Osmolovskaya EA, et al. (2004) Caminibacter profundus sp nov., a novel thermophile of Nautiliales ord. nov within the class ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Micr 54: 41–45.ML MiroshnichenkoS. L'HaridonP. SchumannS. SpringEA Bonch-Osmolovskaya2004Caminibacter profundus sp nov., a novel thermophile of Nautiliales ord. nov within the class ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal vent.Int J Syst Evol Micr544145
- 25. Takai K, Hirayama H, Nakagawa T, Suzuki Y, Nealson KH, et al. (2005) Lebetimonas acidiphila gen. nov., sp nov., a novel thermophilic, acidophilic, hydrogen-oxidizing chemolithoautotroph within the Epsilonproteobacteria, isolated from a deep-sea hydrothermal fumarole in the Mariana Arc. Int J Syst Evol Micr 55: 183–189.K. TakaiH. HirayamaT. NakagawaY. SuzukiKH Nealson2005Lebetimonas acidiphila gen. nov., sp nov., a novel thermophilic, acidophilic, hydrogen-oxidizing chemolithoautotroph within the Epsilonproteobacteria, isolated from a deep-sea hydrothermal fumarole in the Mariana Arc.Int J Syst Evol Micr55183189
- 26. Campbell BJ, Stein JL, Cary SC (2003) Evidence of chemolithoautotrophy in the bacterial community associated with Alvinella pompejana, a hydrothermal vent polychaete. Appl Environ Microb 69: 5070–5078.BJ CampbellJL SteinSC Cary2003Evidence of chemolithoautotrophy in the bacterial community associated with Alvinella pompejana, a hydrothermal vent polychaete.Appl Environ Microb6950705078
- 27. Scott KM, Sievert SM, Abril FN, Ball LA, Barrett CJ, et al. (2006) The genome of deep-sea vent chemolithoautotroph Thiomicrospira crunogena XCL-2. PloS Biol 4: 2196–2212.KM ScottSM SievertFN AbrilLA BallCJ Barrett2006The genome of deep-sea vent chemolithoautotroph Thiomicrospira crunogena XCL-2.PloS Biol421962212
- 28. Jannasch HW, Wirsen CO, Nelson DC, Robertson LA (1985) Thiomicrospira crunogena sp. nov, a colorless, sulfur-oxidizing bacterium from a deep-sea hydrothermal vent. Int J Syst Evol Micr 35: 422–424.HW JannaschCO WirsenDC NelsonLA Robertson1985Thiomicrospira crunogena sp. nov, a colorless, sulfur-oxidizing bacterium from a deep-sea hydrothermal vent.Int J Syst Evol Micr35422424
- 29. Nakagawa S, Takai K, Inagaki F, Hirayama H, Nunoura T, et al. (2005) Distribution, phylogenetic diversity and physiological characteristics of epsilon-Proteobacteria in a deep-sea hydrothermal field. Environ Microbiol 7: 1619–1632.S. NakagawaK. TakaiF. InagakiH. HirayamaT. Nunoura2005Distribution, phylogenetic diversity and physiological characteristics of epsilon-Proteobacteria in a deep-sea hydrothermal field.Environ Microbiol716191632
- 30. Kern M, Mager AM, Simon J (2007) Role of individual nap gene cluster products in NapC-independent nitrate respiration of Wolinella succinogenes. Microbiol Sgm 153: 3739–3747.M. KernAM MagerJ. Simon2007Role of individual nap gene cluster products in NapC-independent nitrate respiration of Wolinella succinogenes.Microbiol Sgm15337393747
- 31. Sievert SM, Scott KM, Klotz MG, Chain PSG, Hauser LJ, et al. (2008) Genome of the epsilonproteobacterial chemolithoautotroph Sulfurimonas denitrificans. Appl Environ Microb 74: 1145–1156.SM SievertKM ScottMG KlotzPSG ChainLJ Hauser2008Genome of the epsilonproteobacterial chemolithoautotroph Sulfurimonas denitrificans.Appl Environ Microb7411451156
- 32. Eppinger M, Baar C, Raddatz G, Huson DH, Schuster SC (2004) Comparative analysis of four Campylobacterales. Nat Rev Microbiol 2: 872–885.M. EppingerC. BaarG. RaddatzDH HusonSC Schuster2004Comparative analysis of four Campylobacterales.Nat Rev Microbiol2872885
- 33. Kang J, Blaser MJ (2006) Bacterial populations as perfect gases: genomic integrity and diversification tensions in Helicobacter pylori. Nat Rev Microbiol 4: 826–836.J. KangMJ Blaser2006Bacterial populations as perfect gases: genomic integrity and diversification tensions in Helicobacter pylori.Nat Rev Microbiol4826836
- 34. Lobry JR (1996) A simple vectorial representation of DNA sequences for the detection of replication origins in bacteria. Biochimie 78: 323–326.JR Lobry1996A simple vectorial representation of DNA sequences for the detection of replication origins in bacteria.Biochimie78323326
- 35. Zhang Y, Romero H, Salinas G, Gladyshev VN (2006) Dynamic evolution of selenocysteine utilization in bacteria: a balance between selenoprotein loss and evolution of selenocysteine from redox active cysteine residues. Genome Biol 7: R94.Y. ZhangH. RomeroG. SalinasVN Gladyshev2006Dynamic evolution of selenocysteine utilization in bacteria: a balance between selenoprotein loss and evolution of selenocysteine from redox active cysteine residues.Genome Biol7R94
- 36. Hayes F (2003) Toxins-antitoxins: Plasmid maintenance, programmed cell death, and cell cycle arrest. Science 301: 1496–1499.F. Hayes2003Toxins-antitoxins: Plasmid maintenance, programmed cell death, and cell cycle arrest.Science30114961499
- 37. Pandey DP, Gerdes K (2005) Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes. Nucleic Acids Res 33: 966–976.DP PandeyK. Gerdes2005Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.Nucleic Acids Res33966976
- 38. Bose M, Barber RD (2006) Prophage Finder: a prophage loci prediction tool for prokaryotic genome sequences. In Silico Biology 6: 223–227.M. BoseRD Barber2006Prophage Finder: a prophage loci prediction tool for prokaryotic genome sequences.In Silico Biology6223227
- 39. Galperin MY (2004) Bacterial signal transduction network in a genomic perspective. Environ Microbiol 6: 552–567.MY Galperin2004Bacterial signal transduction network in a genomic perspective.Environ Microbiol6552567
- 40. Jenal U, Malone J (2006) Mechanisms of cyclic-di-GMP signaling in bacteria. Annu Rev Genet 40: 385–407.U. JenalJ. Malone2006Mechanisms of cyclic-di-GMP signaling in bacteria.Annu Rev Genet40385407
- 41. Zhulin IB, Taylor BL, Dixon R (1997) PAS domain S-boxes in archaea, bacteria and sensors for oxygen and redox. Trends Biochem Sci 22: 331–333.IB ZhulinBL TaylorR. Dixon1997PAS domain S-boxes in archaea, bacteria and sensors for oxygen and redox.Trends Biochem Sci22331333
- 42. Romling U, Gomelsky M, Galperin MY (2005) C-di-GMP: the dawning of a novel bacterial signaling system. Molecular Microbiol 57: 629–639.U. RomlingM. GomelskyMY Galperin2005C-di-GMP: the dawning of a novel bacterial signaling system.Molecular Microbiol57629639
- 43. Keller L, Surette MG (2006) Communication in bacteria: an ecological and evolutionary perspective. Nat Rev Microbiol 4: 249–258.L. KellerMG Surette2006Communication in bacteria: an ecological and evolutionary perspective.Nat Rev Microbiol4249258
- 44. Vendeville A, Winzer K, Heurlier K, Tang CM, Hardie KR (2005) Making ‘sense’ of metabolism: autoinducer-2, LUXS and pathogenic bacteria. Nat Rev Microbiol 3: 383–396.A. VendevilleK. WinzerK. HeurlierCM TangKR Hardie2005Making ‘sense’ of metabolism: autoinducer-2, LUXS and pathogenic bacteria.Nat Rev Microbiol3383396
- 45. Xavier KB, Bassler BL (2003) LuxS quorum sensing: more than just a numbers game. Current Opinion Microbiol 6: 191–197.KB XavierBL Bassler2003LuxS quorum sensing: more than just a numbers game.Current Opinion Microbiol6191197
- 46. Grzymski JJ, Murray AE, Campbell BJ, Kaplarevic M, Gao G, et al. (2008) Metagenome analysis of an extreme microbial symbiosis reveals eurythermal adaptation and metabolic flexibility. P Natl Acad Sci USA 105: 17516–17521.JJ GrzymskiAE MurrayBJ CampbellM. KaplarevicG. Gao2008Metagenome analysis of an extreme microbial symbiosis reveals eurythermal adaptation and metabolic flexibility.P Natl Acad Sci USA1051751617521
- 47. Redfield RJ (2002) Is quorum sensing a side effect of diffusion sensing? Trends Microbiol 10: 365–370.RJ Redfield2002Is quorum sensing a side effect of diffusion sensing?Trends Microbiol10365370
- 48. Patel CN, Wortham BW, Lines JL, Fetherston JD, Perry RD, et al. (2006) Polyamines are essential for the formation of plague biofilm. J Bacteriol 188: 2355–2363.CN PatelBW WorthamJL LinesJD FetherstonRD Perry2006Polyamines are essential for the formation of plague biofilm.J Bacteriol18823552363
- 49. Doig P, de Jonge BL, Alm RA, Brown ED, Uria-Nickelsen M, et al. (1999) Helicobacter pylori physiology predicted from genomic comparison of two strains. Microbiol Mol Biol R 63: 675–707.P. DoigBL de JongeRA AlmED BrownM. Uria-Nickelsen1999Helicobacter pylori physiology predicted from genomic comparison of two strains.Microbiol Mol Biol R63675707
- 50. Karatan E, Duncan TR, Watnick PI (2005) NspS, a predicted polyamine sensor, mediates activation of Vibrio cholerae biofilm formation by norspermidine. J Bacteriol 187: 7434–7443.E. KaratanTR DuncanPI Watnick2005NspS, a predicted polyamine sensor, mediates activation of Vibrio cholerae biofilm formation by norspermidine.J Bacteriol18774347443
- 51. Bomchil N, Watnick P, Kolter R (2003) Identification and characterization of a Vibrio cholerae gene, mbaA, involved in maintenance of biofilm architecture. J Bacteriol 185: 1384–1390.N. BomchilP. WatnickR. Kolter2003Identification and characterization of a Vibrio cholerae gene, mbaA, involved in maintenance of biofilm architecture.J Bacteriol18513841390
- 52. Niba ET, Naka Y, Nagase M, Mori H, Kitakawa M (2007) A Genome-wide approach to identify the genes involved in biofilm formation in E. coli. DNA Res 14: 237–246.ET NibaY. NakaM. NagaseH. MoriM. Kitakawa2007A Genome-wide approach to identify the genes involved in biofilm formation in E. coli.DNA Res14237246
- 53. Reeser RJ, Medler RT, Billington SJ, Jost BH, Joens LA (2007) Characterization of Campylobacter jejuni biofilms under defined growth conditions. Appl Environ Microb 73: 1908–1913.RJ ReeserRT MedlerSJ BillingtonBH JostLA Joens2007Characterization of Campylobacter jejuni biofilms under defined growth conditions.Appl Environ Microb7319081913
- 54. Joshua GW, Guthrie-Irons C, Karlyshev AV, Wren BW (2006) Biofilm formation in Campylobacter jejuni. Microbiology 152: 387–396.GW JoshuaC. Guthrie-IronsAV KarlyshevBW Wren2006Biofilm formation in Campylobacter jejuni.Microbiology152387396
- 55. Kalmokoff M, Lanthier P, Tremblay TL, Foss M, Lau PC, et al. (2006) Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation. J Bacteriol 188: 4312–4320.M. KalmokoffP. LanthierTL TremblayM. FossPC Lau2006Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation.J Bacteriol18843124320
- 56. Bessman MJ, Frick DN, Ohandley SF (1996) The MutT proteins or “nudix” hydrolases, a family of versatile, widely distributed, “housecleaning” enzymes. J Biol Chem 271: 25059–25062.MJ BessmanDN FrickSF Ohandley1996The MutT proteins or “nudix” hydrolases, a family of versatile, widely distributed, “housecleaning” enzymes.J Biol Chem2712505925062
- 57. Miller WG, Parker CT, Rubenfield M, Mendz GL, Wosten MM, et al. (2007) The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri. PLoS ONE 2: e1358.WG MillerCT ParkerM. RubenfieldGL MendzMM Wosten2007The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri.PLoS ONE2e1358
- 58. McLennan AG (2006) The Nudix hydrolase superfamily. Cell Mol Life Sci 63: 123–143.AG McLennan2006The Nudix hydrolase superfamily.Cell Mol Life Sci63123143
- 59. Gabelli SB, Bianchet MA, Ohnishi Y, Ichikawa Y, Bessman MJ, et al. (2002) Mechanism of the Escherichia coli ADP-ribose pyrophosphatase, a nudix hydrolase. Biochemistry 41: 9279–9285.SB GabelliMA BianchetY. OhnishiY. IchikawaMJ Bessman2002Mechanism of the Escherichia coli ADP-ribose pyrophosphatase, a nudix hydrolase.Biochemistry4192799285
- 60. Dunn CA, O'Handley SF, Frick DN, Bessman MJ (1999) Studies on the ADP-ribose pyrophosphatase subfamily of the Nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance. J Biol Chem 274: 32318–32324.CA DunnSF O'HandleyDN FrickMJ Bessman1999Studies on the ADP-ribose pyrophosphatase subfamily of the Nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance.J Biol Chem2743231832324
- 61. Silver S, Phung LT (2005) Genes and enzymes involved in bacterial oxidation and reduction of inorganic arsenic. Appl Environ Microb 71: 599–608.S. SilverLT Phung2005Genes and enzymes involved in bacterial oxidation and reduction of inorganic arsenic.Appl Environ Microb71599608
- 62. Seaver LC, Imlay JA (2001) Alkyl hydroperoxide reductase is the primary scavenger of endogenous hydrogen peroxide in Escherichia coli. J Bacteriol 183: 7173–7181.LC SeaverJA Imlay2001Alkyl hydroperoxide reductase is the primary scavenger of endogenous hydrogen peroxide in Escherichia coli.J Bacteriol18371737181
- 63. Sherrill C, Fahey RC (1998) Import and metabolism of glutathione by Streptococcus mutans. J Bacteriol 180: 1454–1459.C. SherrillRC Fahey1998Import and metabolism of glutathione by Streptococcus mutans.J Bacteriol18014541459
- 64. Hummel CS, Lancaster KM, Crane EJ 3rd (2005) Determination of coenzyme A levels in Pyrococcus furiosus and other Archaea: implications for a general role for coenzyme A in thermophiles. FEMS Microbiol Lett 252: 229–234.CS HummelKM LancasterEJ Crane 3rd2005Determination of coenzyme A levels in Pyrococcus furiosus and other Archaea: implications for a general role for coenzyme A in thermophiles.FEMS Microbiol Lett252229234
- 65. Harris DR, Ward DE, Feasel JM, Lancaster KM, Murphy RD, et al. (2005) Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii. Implications for this disulfide metabolism of anaerobic hyperthermophiles. FEBS J 272: 1189–1200.DR HarrisDE WardJM FeaselKM LancasterRD Murphy2005Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii. Implications for this disulfide metabolism of anaerobic hyperthermophiles.FEBS J27211891200
- 66. Schut GJ, Bridger SL, Adams MW (2007) Insights into the metabolism of elemental sulfur by the hyperthermophilic archaeon Pyrococcus furiosus: characterization of a coenzyme A- dependent NAD(P)H sulfur oxidoreductase. J Bacteriol 189: 4431–4441.GJ SchutSL BridgerMW Adams2007Insights into the metabolism of elemental sulfur by the hyperthermophilic archaeon Pyrococcus furiosus: characterization of a coenzyme A- dependent NAD(P)H sulfur oxidoreductase.J Bacteriol18944314441
- 67. Mussmann M, Hu FZ, Richter M, de Beer D, Preisler A, et al. (2007) Insights into the genome of large sulfur bacteria revealed by analysis of single filaments. PloS Biol 5: 1923–1937.M. MussmannFZ HuM. RichterD. de BeerA. Preisler2007Insights into the genome of large sulfur bacteria revealed by analysis of single filaments.PloS Biol519231937
- 68. Eisen JA, Nelson KE, Paulsen IT, Heidelberg JF, Wu M, et al. (2002) The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium. P Natl Acad Sci USA 99: 9509–9514.JA EisenKE NelsonIT PaulsenJF HeidelbergM. Wu2002The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium.P Natl Acad Sci USA9995099514
- 69. Deckert G, Warren PV, Gaasterland T, Young WG, Lenox AL, et al. (1998) The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature 392: 353–358.G. DeckertPV WarrenT. GaasterlandWG YoungAL Lenox1998The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.Nature392353358
- 70. Mulligan C, Kelly DJ, Thomas GH (2007) Tripartite ATP-independent periplasmic transporters: Application of a relational database for genome-wide analysis of transporter gene frequency and organization. J Mol Microb Biotech 12: 218–226.C. MulliganDJ KellyGH Thomas2007Tripartite ATP-independent periplasmic transporters: Application of a relational database for genome-wide analysis of transporter gene frequency and organization.J Mol Microb Biotech12218226
- 71. Auman AJ, Breezee JL, Gosink JJ, Kampfer P, Staley JT (2006) Psychromonas ingrahamii sp nov., a novel gas vacuolate, psychrophilic bacterium isolated from Arctic polar sea ice. Int J Syst Evol Micr 56: 1001–1007.AJ AumanJL BreezeeJJ GosinkP. KampferJT Staley2006Psychromonas ingrahamii sp nov., a novel gas vacuolate, psychrophilic bacterium isolated from Arctic polar sea ice.Int J Syst Evol Micr5610011007
- 72. Krafft T, Gross R, Kroger A (1995) The function of Wolinella succinogenes psr genes in electron transport with polysulphide as the terminal electron acceptor. Eur J Biochem 230: 601–606.T. KrafftR. GrossA. Kroger1995The function of Wolinella succinogenes psr genes in electron transport with polysulphide as the terminal electron acceptor.Eur J Biochem230601606
- 73. Ma K, Schicho RN, Kelly RM, Adams MW (1993) Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: evidence for a sulfur-reducing hydrogenase ancestor. P Natl Acad Sci USA 90: 5341–5344.K. MaRN SchichoRM KellyMW Adams1993Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: evidence for a sulfur-reducing hydrogenase ancestor.P Natl Acad Sci USA9053415344
- 74. Ma K, Weiss R, Adams MW (2000) Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction. J Bacteriol 182: 1864–1871.K. MaR. WeissMW Adams2000Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction.J Bacteriol18218641871
- 75. Childers SE, Noll KM (1994) Characterization and regulation of sulfur reductase activity in Thermotoga neapolitana. Appl Environ Microb 60: 2622–2626.SE ChildersKM Noll1994Characterization and regulation of sulfur reductase activity in Thermotoga neapolitana.Appl Environ Microb6026222626
- 76. Klimmek O, Kreis V, Klein C, Simon J, Wittershagen A, et al. (1998) The function of the periplasmic Sud protein in polysulfide respiration of Wolinella succinogenes. Eur J Biochem 253: 263–269.O. KlimmekV. KreisC. KleinJ. SimonA. Wittershagen1998The function of the periplasmic Sud protein in polysulfide respiration of Wolinella succinogenes.Eur J Biochem253263269
- 77. Thomas GH, Mullins JGL, Merrick M (2000) Membrane topology of the Mep/Amt family of ammonium transporters. Mol Microbiol 37: 331–344.GH ThomasJGL MullinsM. Merrick2000Membrane topology of the Mep/Amt family of ammonium transporters.Mol Microbiol37331344
- 78. Klotz MG, Stein LY (2008) Nitrifier genomics and evolution of the nitrogen cycle. FEMS Microbiol Lett 278: 146–156.MG KlotzLY Stein2008Nitrifier genomics and evolution of the nitrogen cycle.FEMS Microbiol Lett278146156
- 79. Cabello P, Pino C, Olmo-Mira MF, Castillo F, Roldan MD, et al. (2004) Hydroxylamine assimilation by Rhodobacter capsulatus E1F1 - Requirement of the hcp gene (hybrid cluster protein) located in the nitrate assimilation nas gene region for hydroxylamine reduction. J Biol Chem 279: 45485–45494.P. CabelloC. PinoMF Olmo-MiraF. CastilloMD Roldan2004Hydroxylamine assimilation by Rhodobacter capsulatus E1F1 - Requirement of the hcp gene (hybrid cluster protein) located in the nitrate assimilation nas gene region for hydroxylamine reduction.J Biol Chem2794548545494
- 80. Bergmann DJ, Hooper AB, Klotz MG (2005) Structure and sequence conservation of hao cluster genes of autotrophic ammonia-oxidizing bacteria: Evidence for their evolutionary history. Appl Environ Microb 71: 5371–5382.DJ BergmannAB HooperMG Klotz2005Structure and sequence conservation of hao cluster genes of autotrophic ammonia-oxidizing bacteria: Evidence for their evolutionary history.Appl Environ Microb7153715382
- 81. Klotz MG, Schmid MC, Strous M, Op den Camp HJM, M.S.M. J (2008) Evolution of an octaheme cytochrome c protein family that is key to aerobic and anaerobic ammonia oxidation by bacteria. Environ Microbiol 10: 3150–3163.MG KlotzMC SchmidM. StrousHJM Op den CampJ. M.S.M.2008Evolution of an octaheme cytochrome c protein family that is key to aerobic and anaerobic ammonia oxidation by bacteria.Environ Microbiol1031503163
- 82. Poret-Peterson AT, Graham JE, Gulledge J, Klotz MG (2008) Transcription of nitrification genes by the methane-oxidizing bacterium, Methylococcus capsulatus strain Bath. ISME J 2: 1213–1220.AT Poret-PetersonJE GrahamJ. GulledgeMG Klotz2008Transcription of nitrification genes by the methane-oxidizing bacterium, Methylococcus capsulatus strain Bath.ISME J212131220
- 83. Kostera J, Youngblut MD, Slosarczyk JM, Pacheco AA (2008) Kinetic and product distribution analysis of NO center dot reductase activity in Nitrosomonas europaea hydroxylamine oxidoreductase. J Biol Inorg Chem 13: 1073–1083.J. KosteraMD YoungblutJM SlosarczykAA Pacheco2008Kinetic and product distribution analysis of NO center dot reductase activity in Nitrosomonas europaea hydroxylamine oxidoreductase.J Biol Inorg Chem1310731083
- 84. Lindsay MR, Webb RI, Strous M, Jetten MS, Butler MK, et al. (2001) Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell. Arch Microbiol 175: 413–429.MR LindsayRI WebbM. StrousMS JettenMK Butler2001Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell.Arch Microbiol175413429
- 85. Schmidt I, Look C, Bock E, Jetten MS (2004) Ammonium and hydroxylamine uptake and accumulation in Nitrosomonas. Microbiology 150: 1405–1412.I. SchmidtC. LookE. BockMS Jetten2004Ammonium and hydroxylamine uptake and accumulation in Nitrosomonas.Microbiology15014051412
- 86. Rodionov DA, Dubchak IL, Arkin AP, Alm EJ, Gelfand MS (2005) Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks. PloS Comput Biol 1: e55.DA RodionovIL DubchakAP ArkinEJ AlmMS Gelfand2005Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks.PloS Comput Biol1e55
- 87. Klotz MG, Arp DJ, Chain PSG, El-Sheikh AF, Hauser LJ, et al. (2006) Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707. Appl Environ Microb 72: 6299–6315.MG KlotzDJ ArpPSG ChainAF El-SheikhLJ Hauser2006Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707.Appl Environ Microb7262996315
- 88. Valente FAA, Almeida CC, Pacheco I, Carita J, Saraiva LM, et al. (2006) Selenium is involved in regulation of periplasmic hydrogenase gene expression in Desulfovibrio vulgaris Hildenborough. J Bacteriol 188: 3228–3235.FAA ValenteCC AlmeidaI. PachecoJ. CaritaLM Saraiva2006Selenium is involved in regulation of periplasmic hydrogenase gene expression in Desulfovibrio vulgaris Hildenborough.J Bacteriol18832283235
- 89. Heidelberg JF, Seshadri R, Haveman SA, Hemme CL, Paulsen IT, et al. (2004) The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough. Nat Biotechnol 22: 554–559.JF HeidelbergR. SeshadriSA HavemanCL HemmeIT Paulsen2004The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough.Nat Biotechnol22554559
- 90. Maeder DL, Anderson I, Brettin TS, Bruce DC, Gilna P, et al. (2006) The Methanosarcina barkeri genome: Comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes. J Bacteriol 188: 7922–7931.DL MaederI. AndersonTS BrettinDC BruceP. Gilna2006The Methanosarcina barkeri genome: Comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes.J Bacteriol18879227931
- 91. Larimer FW, Chain P, Hauser L, Lamerdin J, Malfatti S, et al. (2004) Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris. Nat Biotechnol 22: 55–61.FW LarimerP. ChainL. HauserJ. LamerdinS. Malfatti2004Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris.Nat Biotechnol225561
- 92. Deppenmeier U, Johann A, Hartsch T, Merkl R, Schmitz RA, et al. (2002) The genome of Methanosarcina mazei: Evidence for lateral gene transfer between bacteria and archaea. J Mol Microb Biotech 4: 453–461.U. DeppenmeierA. JohannT. HartschR. MerklRA Schmitz2002The genome of Methanosarcina mazei: Evidence for lateral gene transfer between bacteria and archaea.J Mol Microb Biotech4453461
- 93. Wu M, Ren QH, Durkin AS, Daugherty SC, Brinkac LM, et al. (2005) Life in hot carbon monoxide: The complete genome sequence of Carboxydothermus hydrogenoformans Z-2901. PloS Genetics 1: 563–574.M. WuQH RenAS DurkinSC DaughertyLM Brinkac2005Life in hot carbon monoxide: The complete genome sequence of Carboxydothermus hydrogenoformans Z-2901.PloS Genetics1563574
- 94. Meuer J, Kuettner HC, Zhang JK, Hedderich R, Metcalf WW (2002) Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation. P Natl Acad Sci USA 99: 5632–5637.J. MeuerHC KuettnerJK ZhangR. HedderichWW Metcalf2002Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation.P Natl Acad Sci USA9956325637
- 95. Brugna-Guiral M, Tron P, Nitschke W, Stetter KO, Burlat B, et al. (2003) [NiFe] hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: properties, function, and phylogenetics. Extremophiles 7: 145–157.M. Brugna-GuiralP. TronW. NitschkeKO StetterB. Burlat2003[NiFe] hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: properties, function, and phylogenetics.Extremophiles7145157
- 96. Kerby RL, Hong SS, Ensign SA, Coppoc LJ, Ludden PW, et al. (1992) Genetic and physiological characterization of the Rhodospirillum rubrum carbon monoxide dehydrogenase system. J Bacteriol 174: 5284–5294.RL KerbySS HongSA EnsignLJ CoppocPW Ludden1992Genetic and physiological characterization of the Rhodospirillum rubrum carbon monoxide dehydrogenase system.J Bacteriol17452845294
- 97. Bruggemann H, Baumer S, Fricke WF, Wiezer A, Liesegang H, et al. (2003) The genome sequence of Clostridium tetani, the causative agent of tetanus disease. P Natl Acad Sci USA 100: 1316–1321.H. BruggemannS. BaumerWF FrickeA. WiezerH. Liesegang2003The genome sequence of Clostridium tetani, the causative agent of tetanus disease.P Natl Acad Sci USA10013161321
- 98. Mijts BN, Patel BKC (2001) Random sequence analysis of genomic DNA of an anaerobic, thermophilic, halophilic bacterium, Halothermothrix orenii. Extremophiles 5: 61–69.BN MijtsBKC Patel2001Random sequence analysis of genomic DNA of an anaerobic, thermophilic, halophilic bacterium, Halothermothrix orenii.Extremophiles56169
- 99. Fukui T, Atomi H, Kanai T, Matsumi R, Fujiwara S, et al. (2005) Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes. Gen Res 15: 352–363.T. FukuiH. AtomiT. KanaiR. MatsumiS. Fujiwara2005Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes.Gen Res15352363
- 100. Arp DJ, Chain PSG, Klotz MG (2007) The impact of genome analyses on our understanding of ammonia-oxidizing bacteria. Annu Rev Microbiol 61: 503–528.DJ ArpPSG ChainMG Klotz2007The impact of genome analyses on our understanding of ammonia-oxidizing bacteria.Annu Rev Microbiol61503528
- 101. Barnes MH, Leo CJ, Brown NC (1998) DNA polymerase III of Gram-positive eubacteria is a zinc metalloprotein conserving an essential finger-like domain. Biochemistry 37: 15254–15260.MH BarnesCJ LeoNC Brown1998DNA polymerase III of Gram-positive eubacteria is a zinc metalloprotein conserving an essential finger-like domain.Biochemistry371525415260
- 102. Wieczorek A, McHenry CS (2006) The NH2-terminal php domain of the alpha subunit of the Escherichia coli replicase binds the epsilon proofreading subunit. J Biol Chem 281: 12561–12567.A. WieczorekCS McHenry2006The NH2-terminal php domain of the alpha subunit of the Escherichia coli replicase binds the epsilon proofreading subunit.J Biol Chem2811256112567
- 103. Rebeil R, Sun YB, Chooback L, Pedraza-Reyes M, Kinsland C, et al. (1998) Spore photoproduct lyase from Bacillus subtilis spores is a novel iron-sulfur DNA repair enzyme which shares features with proteins such as class III anaerobic ribonucleotide reductases and pyruvate-formate lyases. J Bacteriol 180: 4879–4885.R. RebeilYB SunL. ChoobackM. Pedraza-ReyesC. Kinsland1998Spore photoproduct lyase from Bacillus subtilis spores is a novel iron-sulfur DNA repair enzyme which shares features with proteins such as class III anaerobic ribonucleotide reductases and pyruvate-formate lyases.J Bacteriol18048794885
- 104. White SN, Chave AD, Reynolds GT (2002) Investigations of ambient light emission at deep-sea hydrothermal vents. J Geophysi Res-SE 107: B1.SN WhiteAD ChaveGT Reynolds2002Investigations of ambient light emission at deep-sea hydrothermal vents.J Geophysi Res-SE107B1
- 105. Lombardot T, Kottmann R, Giuliani G, de Bono A, Addor N, et al. (2007) MetaLook: a 3D visualisation software for marine ecological genomics. BMC Bioinformatics 8: 406.T. LombardotR. KottmannG. GiulianiA. de BonoN. Addor2007MetaLook: a 3D visualisation software for marine ecological genomics.BMC Bioinformatics8406
- 106. Hou S, Saw JH, Lee KS, Freitas TA, Belisle C, et al. (2004) Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy. P Natl Acad Sci USA 101: 18036–18041.S. HouJH SawKS LeeTA FreitasC. Belisle2004Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy.P Natl Acad Sci USA1011803618041
- 107. Jolivet E, Corre E, L'Haridon S, Forterre P, Prieur D (2004) Thermococcus marinus sp. nov. and Thermococcus radiotolerans sp. nov., two hyperthermophilic archaea from deep-sea hydrothermal vents that resist ionizing radiation. Extremophiles 8: 219–227.E. JolivetE. CorreS. L'HaridonP. ForterreD. Prieur2004Thermococcus marinus sp. nov. and Thermococcus radiotolerans sp. nov., two hyperthermophilic archaea from deep-sea hydrothermal vents that resist ionizing radiation.Extremophiles8219227
- 108. Company R, Serafim A, Cosson RP, Fiala-Medioni A, Camus L, et al. (2008) Antioxidant biochemical responses to long-term copper exposure in Bathymodiolus azoricus from Menez-Gwen hydrothermal vent. Sci Total Environ 389: 407–417.R. CompanyA. SerafimRP CossonA. Fiala-MedioniL. Camus2008Antioxidant biochemical responses to long-term copper exposure in Bathymodiolus azoricus from Menez-Gwen hydrothermal vent.Sci Total Environ389407417
- 109. Rodriguez H, Drouin R, Holmquist GP, Oconnor TR, Boiteux S, et al. (1995) Mapping of copper hydrogen peroxideiInduced DNA damage at nucleotide resolution in human genomic DNA by ligation-mediated Polymerase Chain Reaction. J Biol Chem 270: 17633–17640.H. RodriguezR. DrouinGP HolmquistTR OconnorS. Boiteux1995Mapping of copper hydrogen peroxideiInduced DNA damage at nucleotide resolution in human genomic DNA by ligation-mediated Polymerase Chain Reaction.J Biol Chem2701763317640
- 110. Daley JM, Vander Laan RL, Suresh A, Wilson TE (2005) DNA joint dependence of Pol X family polymerase action in nonhomologous end joining. J Biol Chem 280: 29030–29037.JM DaleyRL Vander LaanA. SureshTE Wilson2005DNA joint dependence of Pol X family polymerase action in nonhomologous end joining.J Biol Chem2802903029037
- 111. Weller GR, Kysela B, Roy R, Tonkin LM, Scanlan E, et al. (2002) Identification of a DNA nonhomologous end-joining complex in bacteria. Science 297: 1686–1689.GR WellerB. KyselaR. RoyLM TonkinE. Scanlan2002Identification of a DNA nonhomologous end-joining complex in bacteria.Science29716861689
- 112. Shuman S, Glickman MS (2007) Bacterial DNA repair by non-homologous end joining. Nat Rev Microbiol 5: 852–861.S. ShumanMS Glickman2007Bacterial DNA repair by non-homologous end joining.Nat Rev Microbiol5852861
- 113. Kampmann M, Stock D (2004) Reverse gyrase has heat-protective DNA chaperone activity independent of supercoiling. Nucleic Acids Res 32: 3537–3545.M. KampmannD. Stock2004Reverse gyrase has heat-protective DNA chaperone activity independent of supercoiling.Nucleic Acids Res3235373545
- 114. Hsieh TS, Capp C (2005) Nucleotide- and stoichiometry-dependent DNA supercoiling by reverse gyrase. J Biol Chem 280: 20467–20475.TS HsiehC. Capp2005Nucleotide- and stoichiometry-dependent DNA supercoiling by reverse gyrase.J Biol Chem2802046720475
- 115. Kikuchi A, Asai K (1984) Reverse gyrase-a topoisomerase which introduces positive superhelical turns into DNA. Nature 309: 677–681.A. KikuchiK. Asai1984Reverse gyrase-a topoisomerase which introduces positive superhelical turns into DNA.Nature309677681
- 116. LaMarr WA, Yu L, Nicolaou KC, Dedon PC (1998) Supercoiling affects the accessibility of glutathione to DNA-bound molecules: Positive supercoiling inhibits calicheamicin-induced DNA damage. P Natl Acad Sci USA 95: 102–107.WA LaMarrL. YuKC NicolaouPC Dedon1998Supercoiling affects the accessibility of glutathione to DNA-bound molecules: Positive supercoiling inhibits calicheamicin-induced DNA damage.P Natl Acad Sci USA95102107
- 117. Brochier-Armanet C, Forterre P (2007) Widespread distribution of archaeal reverse gyrase in thermophilic bacteria suggests a complex history of vertical inheritance and lateral gene transfers. Archaea 2: 83–93.C. Brochier-ArmanetP. Forterre2007Widespread distribution of archaeal reverse gyrase in thermophilic bacteria suggests a complex history of vertical inheritance and lateral gene transfers.Archaea28393
- 118. Atomi H, Matsumi R, Imanaka T (2004) Reverse gyrase is not a prerequisite for hyperthermophilic life. J Bacteriol 186: 4829–4833.H. AtomiR. MatsumiT. Imanaka2004Reverse gyrase is not a prerequisite for hyperthermophilic life.J Bacteriol18648294833
- 119. McClendon JH (1999) The origin of life. Earth-Science Rev 47: 71–93.JH McClendon1999The origin of life.Earth-Science Rev477193
- 120. Martin W, Russell MJ (2003) On the origins of cells: a hypothesis for the evolutionary transitions from abiotic geochemistry to chemoautotrophic prokaryotes, and from prokaryotes to nucleated cells. Philos T Roy Soc B 358: 59–83.W. MartinMJ Russell2003On the origins of cells: a hypothesis for the evolutionary transitions from abiotic geochemistry to chemoautotrophic prokaryotes, and from prokaryotes to nucleated cells.Philos T Roy Soc B3585983
- 121. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, et al. (1992) Current protocols in molecular biology. New York: Greene Publishing Associates and Wiley-Interscience. FM AusubelR. BrentRE KingstonDD MooreJG Seidman1992Current protocols in molecular biologyNew YorkGreene Publishing Associates and Wiley-Interscience
- 122. Nierman WC, DeShazer D, Kim H, Tettelin H, Nelson KE, et al. (2004) Structural flexibility in the Burkholderia mallei genome. 2004. Proc Natl Acad Sci USA 101: 14246–14251.WC NiermanD. DeShazerH. KimH. TettelinKE Nelson2004Structural flexibility in the Burkholderia mallei genome. 2004.Proc Natl Acad Sci USA1011424614251
- 123. Cline J (1969) Spectrophotometric determination of hydrogen sulfide in natural waters. Limnology Oceanogr 14: 454–458.J. Cline1969Spectrophotometric determination of hydrogen sulfide in natural waters.Limnology Oceanogr14454458
- 124. Markowitz VM, Szeto E, Palaniappan K, Grechkin Y, Chu K, et al. (2008) The integrated microbial genomes (IMG) system in 2007: data content and analysis tool extensions. Nucleic Acids Res 36: D528–D533.VM MarkowitzE. SzetoK. PalaniappanY. GrechkinK. Chu2008The integrated microbial genomes (IMG) system in 2007: data content and analysis tool extensions.Nucleic Acids Res36D528D533
- 125. Altschul S, Madden T, Schaffer A, Zhang JH, Zhang Z, et al. (1998) Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. FASEB J 12: A1326–A1326.S. AltschulT. MaddenA. SchafferJH ZhangZ. Zhang1998Gapped BLAST and PSI-BLAST: A new generation of protein database search programs.FASEB J12A1326A1326
- 126. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL (2006) GenBank. Nucleic Acids Res 34: D16–D20.DA BensonI. Karsch-MizrachiDJ LipmanJ. OstellDL Wheeler2006GenBank.Nucleic Acids Res34D16D20
- 127. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, et al. (2008) The RAST server: Rapid annotations using subsystems technology. BMC Genomics 9: 75.RK AzizD. BartelsAA BestM. DeJonghT. Disz2008The RAST server: Rapid annotations using subsystems technology.BMC Genomics975
- 128. Field D, Garrity G, Gray T, Morrison N, Selengut J, et al. (2008) The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol 26: 541–547.D. FieldG. GarrityT. GrayN. MorrisonJ. Selengut2008The minimum information about a genome sequence (MIGS) specification.Nat Biotechnol26541547
- 129. Haas BJ, Delcher AL, Wortman JR, Salzberg SL (2004) DAGchainer: a tool for mining segmental genome duplications and synteny. Bioinformatics 20: 3643–3646.BJ HaasAL DelcherJR WortmanSL Salzberg2004DAGchainer: a tool for mining segmental genome duplications and synteny.Bioinformatics2036433646
- 130. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596–1599.K. TamuraJ. DudleyM. NeiS. Kumar2007MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0.Mol Biol Evol2415961599
- 131. Dempster EL, Pryor KV, Francis D, Young JE, Rogers HJ (1999) Rapid DNA extraction from ferns for PCR-based analyses. Biotechniques 27: 66–68.EL DempsterKV PryorD. FrancisJE YoungHJ Rogers1999Rapid DNA extraction from ferns for PCR-based analyses.Biotechniques276668
- 132. Suzuki MT, Taylor LT, DeLong EF (2000) Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-nuclease assays. Appl Environ Microb 66: 4605–4614.MT SuzukiLT TaylorEF DeLong2000Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-nuclease assays.Appl Environ Microb6646054614
- 133. Campbell BJ, Waidner LA, Cottrell MT, Kirchman DL (2008) Abundant proteorhodopsin genes in the North Atlantic Ocean. Environ Microbiol 10: 99–109.BJ CampbellLA WaidnerMT CottrellDL Kirchman2008Abundant proteorhodopsin genes in the North Atlantic Ocean.Environ Microbiol1099109
- 134. De la Tour CB, Portemer C, Kaltoum H, Duguet M (1998) Reverse gyrase from the hyperthermophilic bacterium Thermotoga maritima: Properties and gene structure. J Bacteriol 180: 274–281.CB De la TourC. PortemerH. KaltoumM. Duguet1998Reverse gyrase from the hyperthermophilic bacterium Thermotoga maritima: Properties and gene structure.J Bacteriol180274281
- 135. Igarashi N, Moriyama H, Fujiwara T, Fukumori Y, Tanaka N (1997) The 2.8 angstrom structure of hydroxylamine oxidoreductase from a nitrifying chemoautotrophic bacterium, Nitrosomonas europaea. Nature Struct Biol 4: 276–284.N. IgarashiH. MoriyamaT. FujiwaraY. FukumoriN. Tanaka1997The 2.8 angstrom structure of hydroxylamine oxidoreductase from a nitrifying chemoautotrophic bacterium, Nitrosomonas europaea.Nature Struct Biol4276284
- 136. Bergmann DJ, Hooper AB, Klotz MG (2005) Structure and sequence conservation of hao cluster genes of autotrophic ammonia-oxidizing bacteria: Evidence for their evolutionary history. Appl Environ Microb 71: 5371–5382.DJ BergmannAB HooperMG Klotz2005Structure and sequence conservation of hao cluster genes of autotrophic ammonia-oxidizing bacteria: Evidence for their evolutionary history.Appl Environ Microb7153715382
- 137. Field D, Garrity G, Gray T, Morrison N, Selengut J, et al. (2008) The minimum information about a genome sequence (MIGS) specification. Nature Biotech 26: 541–547.D. FieldG. GarrityT. GrayN. MorrisonJ. Selengut2008The minimum information about a genome sequence (MIGS) specification.Nature Biotech26541547