During cell division all chromosomes must be segregated accurately to each daughter cell. Errors in this process give rise to aneuploidy, which leads to birth defects and is implicated in cancer progression. The spindle checkpoint is a surveillance mechanism that ensures high fidelity of chromosome segregation by inhibiting anaphase until all kinetochores have established bipolar attachments to spindle microtubules. Bub1 kinase is a core component of the spindle checkpoint, and cells lacking Bub1 fail to arrest in response to microtubule drugs and precociously segregate their DNA. The mitotic role(s) of Bub1 kinase activity remain elusive, and it is controversial whether this C-terminal domain of Bub1p is required for spindle checkpoint arrest. Here we make a detailed analysis of budding yeast cells lacking the kinase domain (bub1ΔK). We show that despite being able to arrest in response to microtubule depolymerisation and kinetochore-microtubule attachment defects, bub1ΔK cells are sensitive to microtubule drugs. This is because bub1ΔK cells display significant chromosome mis-segregation upon release from nocodazole arrest. bub1ΔK cells mislocalise Sgo1p, and we demonstrate that both the Bub1 kinase domain and Sgo1p are required for accurate chromosome biorientation after nocodazole treatment. We propose that Bub1 kinase and Sgo1p act together to ensure efficient biorientation of sister chromatids during mitosis.
Many human diseases, including birth defects and cancer, are associated with aneuploidy. This is where cells have an incorrect number of chromosomes, because of a failure to segregate their genetic material accurately during cell division. Cells employ many control mechanisms to ensure an extremely high fidelity of chromosome segregation. One way that they do this is to hold the replicated copies of their chromosomes (known as sister chromatids) together until they are all attached properly to microtubules of the mitotic spindle. All pairs of sister chromatids must have one sister attached to each of the two spindle poles, a process known as biorientation. Here we demonstrate that the Bub1 kinase domain acts to target Sgo1 to budding yeast centromeres, and that both of these proteins are required for efficient biorientation of chromosomes in yeast mitosis. Bub1 kinase and Sgo1 functions become particularly important during spindle reassembly after antimicrotubule drug treatment. We propose that this is because the mutant cells fail to respond to kinetochores that are not under tension, and that they are unable to correct syntelic attachments where both sister chromatids attach to microtubules from the same spindle pole.
Citation: Fernius J, Hardwick KG (2007) Bub1 Kinase Targets Sgo1 to Ensure Efficient Chromosome Biorientation in Budding Yeast Mitosis. PLoS Genet 3(11): e213. https://doi.org/10.1371/journal.pgen.0030213
Editor: Orna Cohen-Fix, National Institute of Diabetes & Digestive & Kidney Diseases, United States of America
Received: May 8, 2007; Accepted: October 11, 2007; Published: November 30, 2007
Copyright: © 2007 Fernius and Hardwick. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by a Wellcome Trust PhD Studentship (JF) and a Wellcome Trust Senior Research Fellowship in Basic Biomedical Science (KGH).
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: APC/C, Anaphase Promoting Complex or Cyclosome; ChIP, chromatin immunoprecipitation; CFP, cyan fluorescent protein; GFP, green fluorescent protein; SPB, spindle pole body
The fidelity of chromosome segregation is dependent upon correct bipolar attachment of sister chromatids to the spindle microtubules (for review see ). These attachments are mediated through complex, molecular machines called kinetochores, which assemble at the centromere of each chromosome (for reviews see [2,3]). Accurate chromosome segregation is crucial: any errors lead to aneuploidy, which is characteristic of many diseases and a hallmark of tumour progression [4,5]. Cells have evolved a number of control mechanisms to prevent segregation errors. One of the most important is the spindle checkpoint, which is a surveillance system that tightly regulates the metaphase-to-anaphase transition. It ensures that all kinetochores have established proper bipolar (also known as amphitelic) attachments, where sister kinetochores are attached to microtubules emanating from opposite spindle pole bodies (SPBs), before anaphase is initiated [3,6,7]. The spindle checkpoint consists of a set of conserved proteins (Mad1-3p, Bub1p, Bub3p, and Mps1p [8–10]) that form distinct complexes and localise to unattached kinetochores in a highly ordered manner [11,12]. The downstream target of these proteins is Cdc20p [13,14], an activator of the E3 ubiquitin ligase known as the Anaphase Promoting Complex or Cyclosome (APC/C) (for review see ). Securin (Pds1p in budding yeast) and cyclin B are the key APC/C substrates and polyubiquitination of these, and their ensuing proteolytic destruction, is required for anaphase onset and mitotic exit [16–18]. Thus when the spindle checkpoint is active, the APC/C is inhibited, Securin levels remain high, and anaphase onset is delayed.
The spindle checkpoint responds to unattached kinetochores [19,20] and to lack of tension across sister kinetochores that have yet to achieve proper biorientation . These two responses are linked through the Aurora B kinase homologue, Ipl1p (see  for review). Ipl1p is not required to activate the spindle checkpoint in the response to unattached kinetochores induced by antimicrotubule drugs, but is required to respond to a lack of tension . Ipl1p recognises and breaks defective or inappropriate kinetochore-microtubule attachments, which lack tension, and thereby creates unattached kinetochores . In addition, Ipl1p-dependent phosphorylation of Mad3p ensures full inhibition of Cdc20-APC/C in cells with reduced cohesion .
Other protein kinases (Bub1, BubR1, and Mps1) also play an integral part in the spindle checkpoint (see ). Bub1p is a conserved protein kinase, which is essential for spindle checkpoint arrest. Bub1p forms a complex with Bub3p , binds Mad1p when the checkpoint is active , and is required for localisation of several checkpoint proteins to unattached kinetochores [12,28,29]. It is well established that the N-terminal domains of Bub1p, which include the kinetochore targeting and Bub3p-binding domains, are required for spindle checkpoint arrest [29,30]. However the role of the C-terminal kinase domain remains elusive, although it has been suggested that phosphorylation of Cdc20 by human Bub1 is required to enhance the inhibition of APC/C . Several lines of evidence suggest role(s) for Bub1 in addition to its spindle checkpoint function. Bub1 is required for accurate chromosome segregation in both budding and fission yeasts [29,30] and to maintain ploidy in fission yeast . In addition, there is evidence that Bub1 plays a role in chromosome congression in mammalian cells [28,33].
In this paper we characterise the role of the Bub1p kinase domain in budding yeast mitosis. We show that bub1ΔK cells die rapidly in the presence of microtubule drugs despite being able to initiate and maintain a spindle checkpoint arrest. This rapid cell death is due to chromosome mis-segregation following release from antimicrotubule drugs. In addition, we demonstrate a role for Bub1 kinase in accurate Sgo1p localisation to mitotic centromeres. Sgo1p is the sole budding yeast member of the Shugoshin/MEI-S332 family (for review see ). Members of this family are important protectors of centromeric cohesion, particularly in meiosis [35–38]. However, budding yeast Sgo1p does not regulate cohesion in budding yeast mitosis, and it has been proposed that this protein is a tension sensor at kinetochores . Here we demonstrate that the Bub1 kinase domain and Sgo1p act together to ensure efficient chromosome biorientation.
Although Bub1 kinase activity has been shown to be required for accurate chromosome segregation in both budding and fission yeasts [29,30], the roles of the Bub1 kinase domain have not been clearly established. Indeed, it remains controversial whether the kinase activity is required for a spindle checkpoint arrest, in either organism. In some budding and fission yeast reports, Bub1 kinase activity was thought to be necessary for checkpoint arrest [26,40], but in others it was not [29,30]. This controversy might partly be due to the use of a “kinase-dead” point mutation (K733R), which has since been shown to destabilise budding yeast Bub1p  and could therefore display a phenotype similar to that of a bub1Δ. We generated a novel K733M kinase-dead allele, but found that this protein was also unstable (K. G. Hardwick, unpublished data). Therefore, we chose to carry out a detailed analysis of the role of the Bub1 kinase domain by using a truncated Bub1 kinase allele (containing amino acids 1–608) in Saccharomyces cerevisiae. This allele lacks the whole kinase domain and has already been shown to express a stable protein , and to efficiently bind Bub3p and Mad1p . A similar Bub1 truncation is stable and is able to localise to kinetochores in S. pombe ( and V. Vanoosthuyse, personal communication).
Cells Lacking the Bub1 Kinase Domain Die Rapidly in the Presence of Microtubule Depolymerising Drugs
Spindle checkpoint mutants are hypersensitive to microtubule destabilising drugs because of their inability to arrest in metaphase in response to unattached kinetochores. The precocious separation of sister chromatids gives rise to unequal segregation of chromosomes and aneuploidy, which is lethal in yeast. On rich media containing low concentrations of the microtubule drug benomyl, bub1ΔK cells showed an intermediate sensitivity to the drug compared to other spindle checkpoint mutants. For example, it was clear that the bub1ΔK mutation was not as benomyl sensitive as the complete bub1Δ but that it was more sensitive than mad3Δ (Figure 1A). Spindle checkpoint mutants die rapidly in liquid cultures containing microtubule drugs , so we asked how long bub1ΔK cells remain viable under such conditions. Cells were grown in liquid media containing 30 μg/ml nocodazole and then plated on rich media lacking microtubule drugs. Viability was scored as the percentage of cells able to form colonies. In contrast to wild-type cells, bub1ΔK behaved like bub1Δ and mad2Δ cells and showed rapid death in this viability assay (Figure 1B). After 1 h, 55% of bub1ΔK cells were already inviable. These data show that cells lacking the Bub1 kinase domain are sensitive to microtubule drugs and that they die rapidly, but that bub1ΔK is not a complete loss of function (null) allele.
(A) Wild-type (KH186), mad1Δ (MB076), mad2Δ (KH141), mad3Δ (KH173), bub1Δ (KH127), bub3Δ (MB003), and bub1ΔK (JF098) strains were plated out in 10-fold serial dilutions on YPDA media or on YPDA media containing 8 μg/ml benomyl.
(B) The indicated strains were grown in YPDA media containing 30 μg/ml nocodazole, and the viability of the cells was measured as percentage of cells able to form colonies on YPDA media lacking microtubule drugs.
(C) Wild-type (JF004), bub1ΔK (JF125), bub1Δ (JF140), and mad3Δ (EK013) strains containing a GFP-marked chromosome were synchronised in G1 using α-factor, then released into YPDA with 30 μg/ml nocodazole at 23 °C. We tested ability of the cells to maintain a spindle checkpoint arrest by scoring cells that could keep their GFP-marked sister chromatids cohesed for 3 h in nocodazole (i.e., one GFP dot). The percentage of cells with two GFP dots was counted at the release from G1 (grey bars) and after 3 h in nocodazole (black bars) (n = 100 cells for each repeat experiment). Error bars indicate standard deviation.
(D) Wild-type (JF004) and bub1ΔK (JF125) strains containing Pds1-18myc were arrested in G1 using α-factor and synchronously released into YPDA media or YPDA media containing 30 μg/ml nocodazole at 23 °C. Samples were taken at indicated times. Levels of Pds1 were monitored by immunoblotting using A14 α-myc antibody and α-PGK1 as a loading control.
The Bub1 Kinase Domain Is Dispensable for a Robust Spindle Checkpoint Arrest in Response to Unattached Kinetochores
bub1ΔK cells could be sensitive to microtubule depolymerising drugs for various reasons. They may be unable to arrest in mitosis, or they may fail to recover properly after spindle checkpoint arrest. To distinguish between these possibilities, we examined cell morphology and the level of sister-chromatid cohesion in bub1ΔK cells in response to microtubule drugs. First we performed a morphological assay scoring rebudding of cells on plates containing 20 μg/ml and 80 μg/ml benomyl (see Figure S1). Both wild-type and bub1ΔK cells remained large-budded (which is an indication of mitotic arrest) for up to 6 h on benomyl, compared to the spindle checkpoint mutants, mad2Δ and bub1Δ, which did not respond to microtubule depolymerisation and rebudded prematurely. This confirms the previous report of a large-budded arrest for bub1ΔK cells in nocodazole . To monitor sister-chromatid cohesion directly, we used strains expressing lacI-green fluorescent protein (GFP) and containing a lac-operator array on Chromosome IV, thereby marking this chromosome with GFP. This enabled us to visualize whether chromatids were still cohesed (one GFP dot) or had separated prematurely (two GFP dots) in the presence of antimicrotubule drugs. Cells were synchronized in G1, using the mating pheromone α-factor, and released into media containing nocodazole. bub1ΔK cells behaved like wild type and efficiently maintained sister-chromatid cohesion for 3 h in cultures containing nocodazole (Figure 1C). As a final test of mitotic arrest, we asked whether Securin (Pds1-myc) was stabilized in the presence of nocodazole in bub1ΔK cells. This was the case (Figure 1D), confirming that the bub1ΔK cells efficiently respond to microtubule depolymerisation and initiate and maintain a robust spindle checkpoint arrest.
The Bub1 Kinase Domain Is Necessary for the Checkpoint Response to Reduced Cohesion
In the assays described above, we used antimicrotubule drugs to activate the spindle checkpoint pathway that recognizes unattached kinetochores. Complete microtubule depolymerisation is not a very common physiological situation, and we were therefore interested to analyse bub1ΔK cells in other situations. Ipl1 kinase activates the spindle checkpoint by creating unattached kinetochores in response to mutations in several kinetochore components that were thought to create reduced tension at centromeres . We wondered whether the kinase domain of Bub1 is required to arrest cells containing such defective kinetochores. In contrast to an ipl1–321, mtw1–1 double mutant, cells containing the mtw1–1 mutation in combination with bub1ΔK were able to respond to the kinetochore defect and stabilise Pds1-myc (Figure 2A). Similar results were obtained when using the more severe ndc80–1 kinetochore mutant in combination with bub1ΔK (unpublished data). These results show that Bub1 kinase is not required to “sense” these defective kinetochores, nor to activate Ipl1p in these mutants, nor to respond to the unattached kinetochores that Ipl1p kinase activity creates.
(A) Wild-type (JF004), mtw1–1 (SBY1646), mtw1–1, bub1ΔK (JF100), and mtw1–1, ipl1-321 (SBY1724) strains carrying the temperature-sensitive allele mtw1–1 and Pds1-18Myc were synchronised in G1 for 2.5 h then incubated at the restrictive temperature 36 °C for 30 min before release. The levels of Pds1 were monitored by immunoblotting with A14 anti-myc antibody and anti-PGK1 as a loading control.
(B) Wild-type (VBI545), bub1ΔK (JF023), mad2Δ (VBI560), and sgo1Δ (JF224) strains, containing Pds1-myc, were synchronised in G1 with α-factor in media containing 2% galactose and 2% raffinose to maintain Mcd1 expression. Mcd1 was then turned off by incubating them in media containing glucose and α-factor for 3 h. The cells were then released into rich media containing glucose, and samples for immunoblotting were taken at indicated times. Pds1 and PGK1 levels were monitored as previously described.
(C) Wild-type (YBS151), bub1ΔK (JF110), and mad2Δ (YBS406) strains, carrying a LEU2-marked linear minichromosome (LMC) were plated in 10-fold serial dilutions on −LEU plates and on −LEU plates with 10 μg/ml doxycycline. In this strain doxycycline represses a dominant CDC20 allele that is insensitive to the spindle checkpoint proteins . Therefore this CDC20 allele makes cells insensitive to the presence of LMCs. These LMCs are thought to be unable to withstand spindle forces and separate producing kinetochore-microtubule attachments that are no longer under tension. In the presence of doxycycline, wild-type cells grow very poorly because of their checkpoint response to the LMCs, but the bub1ΔK mutant grows far better, indicating that their ability to respond to attachments lacking tension is impaired.
Ipl1p (Aurora kinase), Mad3p phosphorylation, and Sgo1p have all been demonstrated to be necessary for the checkpoint response to reduced cohesion, even though they are not necessary for the response to unattached kinetochores [23,25,39]. To test whether the Bub1 kinase domain is necessary for the response to reduced cohesin, bub1ΔK,GAL-MCD1 cells were synchronised in G1, depleted for cohesin by turning off MCD1 expression with glucose addition, and then released into the cell cycle. Immunoblotting for Securin levels (Pds1) showed that neither bub1ΔK nor sgo1 mutants were able to maintain a metaphase arrest as well as wild-type cells (Figure 2B). They did not degrade Pds1p quite as quickly as the mad2Δ control, but we believe that was because they grow more slowly, most likely because of their aneuploidy phenotype. We also analysed the ability of bub1ΔK cells to grow in the presence of poorly segregating linear chromosomes. Such chromosomes have been shown to delay mitosis in a checkpoint-dependent manner  and were employed in a screen that identified sgo1 alleles . For the screen, the linear chromosome-induced delay becomes lethal as the strains also contain CDC28-VF, a mutation in CDC28/CDK1 that reduces APC activity. This lethality is rescued by checkpoint mutations, as no mitotic delay is imposed. Figure 2C shows that bub1ΔK cells grow far better than wild type in the presence of the short linear chromosomes, indicating that this checkpoint response is also defective. We conclude that whilst the Bub1 kinase domain is not necessary for the response to unattached or defective kinetochores, it is necessary for the budding yeast checkpoint response to a lack of tension at mitotic kinetochores.
The above experiments strongly suggest that the C-terminal kinase domain of Bub1 is not required to initiate or maintain spindle checkpoint arrests induced by unattached or defective kinetochores, but that it is necessary to respond to a lack of tension. The bub1ΔK phenotype closely mirrors that of sgo1Δ, which is also necessary for the tension response  but not the response to mtw1–1- or ndc80–1-induced kinetochore defects . The bub1ΔK and sgo1Δ phenotypes differ from that displayed by ipl1 mutants. Ipl1p is required for the tension response but also for the response to mtw1–1- or ndc80–1-induced kinetochore defects , and Ipl1p is an essential protein .
The Bub1 Kinase Domain Plays a Role in Sgo1 Localisation
Bub1p is important for Sgo1p localisation at centromeres in meiosis in budding and fission yeast [36,37,44] and regulates Sgo1 localisation in mitosis in human cells [45,46]. Because of this and the similarity in the bub1ΔK and sgo1Δ phenotypes, we tested whether the Bub1 kinase domain has a role in localising Sgo1p to kinetochores in budding yeast mitosis. Unfortunately Sgo1-GFP gives a rather weak signal, so we did this by performing immunofluorescence on fixed chromosome spreads. We used a strain containing Ndc10-6HA to label kinetochores and asked whether Sgo1-9Myc colocalised with Ndc10p in wild type, bub1Δ, and bub1ΔK cells. We analysed unbiased populations of cycling cells (see Figure S2) and cultures that had been arrested in mitosis for 3 h with nocodazole. Whilst wild-type cells showed multiple foci with overlapping localisation for Sgo1p and Ndc10p in many spreads, this was rarely the case for bub1ΔK cells (Figure 3A). Categorization revealed such colocalisation of Sgo1p and Ndc10p in only 13% of mutant cells (compared with 2% in wild type). In bub1ΔK cells there were often one or at most two bright punctate signals for Sgo1p. To determine whether these could be SPBs, we carried out double label staining for Sgo1-9Myc and the 110-kD component of the SPB. Colocalization was observed in many cells (Figure 3B), suggesting that significant levels of Sgo1p localise to the SPB in the bub1ΔK mutant. We cannot rule out the possibility that some of this signal is due to centromeres that remain associated with the SPB in these nocodazole-treated cells. To confirm the decreased association of Sgo1p with kinetochores in bub1ΔK cells, we employed chromatin immunoprecipitation (ChIP). We reproducibly observed decreased association of Sgo1p and centromeres, using both centromeric and pericentromeric primer sets, in bub1ΔK cells (Figure 3C–3E).
(A) Wild-type (AMY1110) and bub1ΔK (JF038) strains containing Sgo1-9Myc and Ndc10-6HA (to mark the kinetochores) were arrested in metaphase with 15 μg/ml nocodazole and 30 μg/ml benomyl at 23 °C for 3 h. Chromosome spreads were performed and stained with α-myc antibody (CM-100), α-HA antibody (HA11), and DAPI to recognize the DNA. Spreads with clear Ndc10-6HA staining were categorized as showing colocalization with Sgo1-9myc only if they contained multiple (>2) overlapping foci. Fifty spreads per strain were analysed.
(B) Sgo1-9myc colocalises with the SPB in the bub1ΔK mutant. Spreads were prepared as in (A) and stained with α-myc antibody, anti-Spc110 antibody to detect SPBs, and DAPI. Scale bar represents 2 μm.
(C) Schematic of primers sets for Sgo1-6HA ChIP analysis showing the CEN3, centromeric region; R3, pericentromeric region; and c281, the negative arm region.
(D) PCR on ChIPs of “no tag” (KH186), Sgo1-6HA (AMY209), and bub1ΔK, Sgo1-6HA (JF211) strains arrested with 15 μg/ml nocodazole and 30 μg/ml benomyl at 23 °C for 3 h, showing reduced Sgo1p levels associated with CEN3 and R3 regions in the bub1ΔK mutant compared to wild type.
(E) The graph shows quantification of the ChIP data from the “no tag” (KH186), wild-type (AMY209), and bub1ΔK (JF211) strains. The “binding ratio” was calculated as a ratio of the ChIP PCR signal to the PCR signal from a 1:500 diluted input fraction. The error bars indicate standard deviation (SD) of the mean from five different PCR reactions from two separate experiments.
These results confirm that the kinase domain of Bub1p plays an important role in localising Sgo1p to budding yeast centromeres in mitosis. This could explain why the sgo1Δ and bub1ΔK strains display such similar phenotypes.
bub1ΔK Cells Mis-Segregate Chromosomes during Recovery from a Checkpoint Arrest
The fact that bub1ΔK cells are hypersensitive to antimicrotubule drugs, yet able to arrest efficiently, suggested to us that the Bub1 kinase domain could be required for proper recovery from spindle damage. To test this, we first asked whether they have increased chromosome loss following nocodazole arrest. We followed chromosome segregation during the first anaphase after nocodazole release, using the GFP-marked chromosome strain described above. Accurate chromosome segregation should give rise to one GFP spot in each daughter cell. We arrested cells in metaphase for 3 h with nocodazole then released them into anaphase. After 30 min, cells were fixed and stained with α-tubulin antibody to monitor spindle elongation. The results showed 33% mis-segregation of the GFP-marked chromatids in bub1ΔK cells (Figure 4A, lower panel) compared to 0.2% in wild type (Figure 4A, upper panel).
(A) Wild-type (JF004) and bub1ΔK (JF125) cells were released from G1 into media containing 30 μg/ml nocodazole and incubated at 23 °C for 3 h. Cells were subsequently released into anaphase by washing out the nocodazole. Samples were fixed in 3.7% formaldehyde for 1 h, 30 min after release, and stained with α-GFP (GFP-marked chromosome) and α-tubulin (red spindle) antibodies. DNA was stained with DAPI (blue). Percentage of nondisjunction of the GFP-marked chromatid at the first anaphase following nocodazole arrest was 2% in wild-type cells and 33% in bub1ΔK cells (n ≥ 50 anaphase cells). Scale bar represents 3 μm.
(B) Wild-type (JF004) and bub1ΔK (JF125) strains were synchronised in G1 as previously described and cells with one GFP dot (empty triangle) versus two dots (filled triangle) were counted (n = 400). Scale bar represents 2 μm.
(C) Cells from (B) were then released and incubated in media containing 30 μg/ml nocodazole at 23 °C for 3 h and released into media containing α-factor to score cells in the following G1. The number of cells with one GFP dot versus two dots were scored (n = 400).
(D) Wild-type (KH186), bub1Δ (KH127), bub1ΔK (JF098), sgo1Δ (JF188), and bub1Δ, sgo1Δ (JF185) strains were plated out in 10-fold serial dilutions on rich media and on rich media containing 8 μg/ml benomyl.
(E) Strains carrying the SUP11 artificial chromosome were grown overnight in CSM-URA media, then diluted back to OD600 0.2 and grown in YPDA media at 30 °C for 3 h. Cells were then plated out on YPD at a density of ∼500 cells per plate. Only colonies that were at least half red were scored for losing the test chromosome at the first division.
We then used the same strain to score a large number of cells for chromosome mis-segregation and compared unchallenged G1 cells with G1 cells that had been released from a nocodazole arrest. Cells were arrested in G1 using α-factor, and the number of cells with one GFP foci (Figure 4C, empty triangle) versus two GFP foci (filled triangle) were counted. As expected, most cells had one GFP dot, representing one copy of Chromosome IV, in both wild-type (0% had two GFP dots, n = 400) and bub1ΔK cells (2% had two GFP dots, n = 400). The small number of bub1ΔK cells with two copies of this chromosome (Figure 4B) reflects a background level of aneuploidy, frequently observed in bub1 mutants. However, when cells were released from G1 into media containing nocodazole for 3 h, then released and trapped in the following G1, there was a marked increase in the number of cells containing two GFP foci in bub1ΔK (30% had two GFP dots, n = 400) compared to wild type (where only 4% had two GFP dots, n = 400) (Figure 4C). This confirms that there was a significant defect in segregating this chromosome faithfully during the anaphase following nocodazole release. Because of the high incidence of chromosome mis-segregation following treatment with nocodazole, and considering that we only scored one of 16 budding yeast chromosomes in this analysis, we propose that the reason why bub1ΔK cells are sensitive to antimicrotubule drugs, despite showing capacity to arrest in metaphase, is because of chromosome loss.
To more accurately quantitate their chromosome-loss rate, and to compare it with that of sgo1Δ mutants, we employed sectoring assays, which have previously been used to analyse many mitotic and checkpoint mutants [30,47]. We used a strain containing a nonessential test chromosome that carries the SUP11 (ochre-suppressing tRNA) gene that makes colonies that are normally red, because of the ade2–1 mutation, white. We scored loss of this chromosome at the first division by counting colonies that are half red and half white. Consistent with published data , in an unchallenged cell cycle, bub1Δ cells lost the test chromosome at a rate of 41 per 1,000 divisions compared to 0.5 per 1,000 in wild type. bub1ΔK cells also showed chromosome loss in a normal mitosis, but at a lower rate than bub1Δ, at 28 per 1,000 divisions (Figure 4E). The loss rate in sgo1Δ was very similar: 32 per 1,000 divisions.
If the Bub1 kinase domain and Sgo1p carry out the same function, one might predict little, if any, synthetic phenotype when combining the two mutations in bub1ΔK, sgo1Δ cells. Alternatively, if the double mutant was significantly sicker then that would suggest that the Bub1 kinase domain has other functions, in addition to Sgo1p targeting. Our analysis of the double mutant, both in terms of sensitivity to microtubule drugs and chromosome-loss rates, strongly supports the former option. We found that the double mutant is no more benomyl sensitive than the bub1ΔK strain (Figure 4D) and that the rate of chromosome loss was no higher than that of the bub1ΔK and sgo1Δ single mutants (Figure 4E).
These results show that the Bub1 kinase domain plays a role in chromosome segregation that becomes very important upon challenge with microtubule drugs. The lack of a synthetic genetic interaction suggests that the Bub1 kinase domain function is closely related to that of Sgo1p, consistent with the idea that the major role of the kinase is to efficiently target Sgo1p to centromeres (Figure 3). Note, bub1Δ cells have an even higher chromosome-loss rate because, in addition to their segregation defects, these cells have also lost their ability to checkpoint arrest.
The Bub1 Kinase Domain Ensures Proper Chromosome Biorientation
As bub1ΔK cells have chromosome segregation defects, we tested whether they displayed kinetochore attachment defects. To do this we employed a bub1ΔK strain containing Tub1-cyan fluorescent protein (CFP) to label spindle microtubules and Mtw1-3xGFP to mark all kinetochores. Cells were arrested in mitosis with benomyl and nocodazole, which were then washed out, and the ensuing anaphase analysed. We identified no more unattached kinetochores (indicated by Mtw1-GFP foci off the spindle axis) in bub1ΔK cells than in wild-type cells (8%, see Figure 5A). Similar results, with low levels of unattached kinetochores, were obtained for sgo1Δ cells. ndc80–1 mutants were used as positive controls for this experiment, and as expected these contain many unattached kinetochores at their restrictive temperature (Figure 5A [24,48]). From this we conclude that bub1ΔK and sgo1Δ cells do not display significant numbers of unattached kinetochores, even after spindle ablation and re-formation.
(A) We analysed the indicated strains for unattached kinetochores following nocodazole treatment. Wild-type (SBY4340), bub1ΔK (JF123), sgo1Δ (JF202), and ndc80–1 (SBY4342) cells were arrested in media containing 30 μg/ml benomyl and 15 μg/ml nocodazole for 3 h at 23 °C. The microtubule drugs were then washed out three times and released into rich media at 30 °C for 20 min. The control ndc80–1 cells were grown at 36 °C for 1 h before fixation. Cells were then fixed in 3.7% formaldehyde for 5 min. We scored cells with kinetochores (Mtw1-3GFP staining) off the spindle axis as “unattached kinetochores.” All strains, apart from the ndc80–1, had mostly attached kinetochores aligned on the spindle. A total of 8% of wild-type cells contained unattached kinetochores at this timepoint (n = 212); bub1ΔK, 8% (n = 252); and sgo1Δ, 4% (n = 157) compared to ndc80–1, which had 79% (n = 96) unattached kinetochores. Right hand panel shows examples of unattached kinetochores for each strain. Scale bar represents 2 μm.
(B) Schematic of the principle of the experiment showing GFP marked centromere “breathing” upon chromosome biorientation (i.e., two green dots).
(C) Images of separated SPBs (Spc42-Tomato) containing either one GFP foci in between two SPBs (empty white triangle, scored as nonbioriented) or one GFP foci by one SPB (red empty triangle, scored as nonbioriented) or finally two GFP foci between two SPBs (filled triangle, scored as bioriented chromosome). Scale bar represents 2 μm.
(D) Wild-type (JF152), bub1ΔK (JF154), sgo1Δ (JF156), and bub1ΔK,sgo1Δ (JF202) strains carrying cenIV-GFP, Met-Cdc20, and Spc42-Tomato were synchronised in G1 for 3 h and then depleted for Cdc20 by incubating them in α-factor and 8 mM methionine for 2 h before releasing them into media containing 30 μg/ml benomyl, 15 μg/ml nocodazole, and 8 mM methionine at 23 °C for 3 h. The nocodazole was then washed out and samples were taken at indicated times. Only cells that had separated SPBs were counted for each sample (n = 100) and scored for biorientated chromatids (two GFP foci between two SPBs) versus nonbioriented chromatids (one GFP foci between two SPBs and one GFP foci by one SPB). Error bars indicate standard deviation. Standard deviations are based on five separate experiments with all strains apart from sgo1Δ,bub1ΔK double mutant strain, which was used in two separate experiments.
(E) This graph represents the cells in which the single GFP dot resided next to the SPB (red triangle) as opposed to in between the SPBs (white empty triangle), calculated as a percentage of the total cells with only one GFP dot. The data plotted are the average from two separate experiments.
Next we wanted to test whether the bub1ΔK cells contain more subtle kinetochore attachment problems, such as defects in biorientation. If, during spindle reassembly, both sister kinetochores attach to microtubules from the same spindle pole (syntelically) perhaps the bub1ΔK cells would be unable to detect or correct this defect. Note syntelic attachments are unlikely to result in tension across sister kinetochores. To test this possibility, we used strains that carry an array of tet operators integrated only 2 kb from the centromere on Chromosome IV and express a Tet repressor-GFP fusion protein. In addition, their spindle poles are marked with Spc42-tomato, and the strain has a methionine repressible promoter for CDC20 (pMET-CDC20). By depleting Cdc20p, we can induce a metaphase arrest that is independent of spindle damage and the checkpoint. Using such strains we could directly test the ability of cells to establish proper bipolar attachment after nocodazole treatment. Amphitelic attachments were visualised as pairs of GFP-centromere (CEN) spots pulled apart by the microtubule forces of the bipolar spindle (see Figure 5B) [49–51], whereas monotelic or syntelic attachments (both monopolar) remained as single GFP-CEN spots, on the spindle axis. Cells were arrested in G1, depleted of Cdc20p, and then released into media containing benomyl and nocodazole for 3 h. The microtubule drugs were then washed out, cells kept in metaphase by continued repression of Cdc20p, and the level of biorientation was scored as two GFP dots in between two red SPBs (filled white triangle, Figure 5C). This procedure gave us the relative levels of biorientation, although these numbers will be underestimated, since, at the time of fixation, some cells that have established biorientation will only show one GFP dot because of the “breathing” characteristics of mitotic centromeres [49,50]. Quantitation of these images revealed a significant defect in establishing proper biorientation in both bub1ΔK and sgo1Δ mutants, after nocodazole release, indicating that Bub1 kinase and Sgo1p both work to ensure efficient chromosome biorientation (Figure 5D). In addition, the bub1ΔK,sgo1Δ double mutant showed a very similar quantitative defect in biorientation to the two single mutants, suggesting that the Bub1 kinase domain and Sgo1p are necessary for the same biorientation function.
The position of the chromosomes in these images, relative to the SPBs (Figure 5E), is consistent with them being syntelically attached: both sisters are attached to the same spindle pole and therefore localise very close to it. We have carried out live-cell analysis of these cells (unpublished data) in which we scored the numbers of cells in which the GFP-marked chromosomes were “breathing” on a short mitotic spindle. Whilst 88% of chromosomes were observed to breathe in wild-type cells, only 56% did in the bub1ΔK and sgo1Δ mutants during the time observed. We conclude that these chromosomes are most likely syntelically attached, rather than being amphitelic attachments that fail to be stretched, and that the bub1ΔK and sgo1Δ mutants are unable to correct these attachments. Such a defect could account for much of the chromosome loss detailed in Figure 4. These findings agree well with recent live-cell imaging from the Murray lab showing that sgo1–100 mutants frequently fail to correct syntelic attachments .
Bub1 Kinase Is Not Required for Most Spindle Checkpoint Arrests, but Is Required for the Response to Reduced Cohesion
In this study we have carried out a detailed analysis of whether the kinase domain of Bub1 is required for a robust spindle checkpoint, using a truncated Bub1 kinase allele. Note, our truncation removes the last 413 residues of Bub1p (residues 609–1,021), which include 83 residues before the start of the conserved kinase domain. bub1ΔK cells showed sensitivity to benomyl, although not as severe as bub1Δ cells, and died rapidly in a nocodazole viability assay. However, our data clearly show that the Bub1 kinase domain is not required to initiate or maintain spindle checkpoint arrests induced by unattached or defective kinetochores. Robust arrests were observed in the presence of antimicrotubule drugs (either benomyl or nocodazole), and in the mtw1–1 and ndc80–1 kinetochore mutants (Figure 2A). In addition, we have found that the Bub1 kinase domain is unnecessary for the arrest induced by overexpression of the Mps1 protein kinase (GAL-MPS1, unpublished data and ). Thus, the Bub1 kinase domain is not necessary for a wide range of spindle checkpoint arrests.
However, like Sgo1p  and Ipl1p , the Bub1 kinase domain is necessary to delay anaphase onset in cells with reduced cohesion (Figure 2B). The simplest interpretation of this result is that these three functions are all needed for the checkpoint response to a lack of tension at kinetochores.
The Sensitivity of bub1ΔK Cells to Microtubule Drugs Is Due to Chromosome Mis-Segregation after the Release from Nocodazole
In agreement with previous work  we found that bub1ΔK cells display chromosome mis-segregation in an unperturbed mitosis, at a level between those of wild-type and bub1Δ cells. The bub1ΔK chromosome-loss rate becomes far higher upon spindle damage (Figure 4). We have also demonstrated that bub1ΔK cells die rapidly when released from nocodazole. Importantly, this was not because of an inability to arrest in nocodazole, but rather to an inability to segregate chromosomes accurately upon spindle reassembly (see Figures 4 and 5). We propose that the Bub1 kinase domain has a role in regulating chromosome segregation every cell cycle, and that this role becomes particularly important after spindle damage. Such segregation defects could reflect the inability of bub1ΔK cells to respond to a lack of tension at kinetochores (Figure 2).
Our genetic studies support the idea that Bub1 kinase and Sgo1p act in the same pathway: whilst the complete gene deletions, bub1Δ and sgo1Δ are synthetically sick , we could detect no significant synthetic interaction between bub1ΔK and sgo1Δ in chromosome-loss rate (Figure 4E) sensitivity to antimicrotubule drugs (Figure 4D) or in the biorientation assay (Figure 5D). The simplest interpretation is that the important functions of Bub1 kinase are all Sgo1-dependent, and therefore already defective in the sgo1Δ. We propose that the complete gene double deletion (bub1Δ,sgo1Δ) is sicker because they are also unable to checkpoint arrest, which is supported by the finding that mad2Δ and sgo1Δ are also synthetically very sick .
Sgo1p Is Mislocalised in bub1ΔK Cells
The close resemblance of the phenotypes between bub1ΔK and sgo1Δ cells encouraged us to investigate further whether they are part of the same pathway. Our data indicate that in budding yeast mitosis the kinase domain of Bub1p does play an important role in accurate Sgo1p localisation (Figure 3). Chromosome spreads and ChIP show that there were reduced levels of Sgo1 at centromeres, in strains lacking the Bub1 kinase domain.
In some experiments a slight increase in Sgo1p levels was observed on chromosome arms. If true for the chromosome as a whole, this finding would be consistent with observations in tissue culture where depletion of hsBub1 lead to hsSgo1 localizing to the chromosome arms . In vertebrates this protects cohesion along the chromosome arms and perturbs sister-chromatid separation. However, in budding yeast there is no evidence that Sgo1 protects cohesin in mitotic cells, and when we analysed cohesin (Mcd1) cleavage in our bub1ΔK mutant (Figure S3) we saw no significant effect. General chromosome staining was not apparent in spreads of mitotic bub1ΔK cells, but we did frequently observe Sgo1p at spindle poles. The reason for this SPB localisation is currently unclear.
Overall, our data agree with work from other groups showing a role for Bub1 in localisation of Sgo1 to centromeres in mitosis in human cells [45,46], and in fission yeast . Data are also in agreement with the finding that Bub1 kinase activity is required for centromeric localisation of Sgo1p (and the protein phosphatase PP2A sub-unit Rts1p) in budding yeast meiosis . That study also showed that Bub1p localisation is independent of Sgo1p and PP2A .
Is Sgo1 a Bub1 Kinase Substrate?
Because sgo1Δ and bub1ΔK cells have such similar phenotypes and Sgo1p is mislocalised in bub1ΔK, Sgo1p is a strong candidate to be a mitotic Bub1 kinase substrate. We have carried out preliminary experiments showing that Sgo1p is a phosphoprotein, but there is little if any effect on the phosphorylation state of Sgo1p in bub1ΔK mutants (J. Fernius, unpublished data). We also failed to observe a gel mobility change for fission yeast Sgo2p in bub1 mutants (V. Vanoosthuyse, personal communication). Therefore we think it is likely that there is an unknown Bub1 kinase substrate (Factor X in our models, see Figure 6) that is required for Sgo1p targeting to centromeres.
(A) Simple linear pathway: Bub1 kinase phosphorylates Factor X in such a way that Sgo1 is efficiently targeted to centromeres. This in turn ensures efficient recruitment of the Passenger proteins, which act to monitor and correct any inappropriate kinetochore-microtubule attachments.
(B) Parallel pathways: Bub1 kinase, Sgo1p, and the Passenger proteins act in a concerted fashion to ensure efficient biorientation of sister kinetochores. This model better explains why localization of the different proteins are not entirely dependent upon one another. It also suggests that Bub1 kinase, and/or Sgo1p, might act to break inappropriate kinetochore attachments independently of Aurora kinase.
Amongst the candidates for Factor X are the Chromosomal Passenger proteins (Aurora B, INCENP, and Survivin, see  for review). In Drosophila meiosis, incenp mutants perturb MEI-S332 (Sgo) localisation, and MEI-S332 was shown to be a good Aurora B substrate . In budding yeast meiosis, Ipl1 kinase and the monopolin complex are key regulators of kinetochore orientation . However, only partial perturbation of Sgo1p was observed upon Ipl1p depletion, and it was shown that Sgo1p actually recruits Ipl1p to meiotic centromeres . Fission yeast studies also demonstrated an interdependence between Passenger proteins and Sgo2p targeting to centromeres, and in those experiments Survivin appears to be the Passenger protein most closely linked to Sgo2p [58,59].
Because of these links with the Passenger proteins, we have carried out preliminary experiments, looking at the Ipl1p and Sli15p in budding yeast mitotic cells that lack the Bub1 kinase domain. Whilst there may be subtle effects on the efficiency of recruitment of the Passenger proteins to mitotic centromeres, we find these to be far less significant than the effects on Sgo1p (Figure S4, and unpublished data). Thus we doubt that Factor X is one of the Passenger proteins (see models in Figure 6). Experiments to identify this factor are ongoing.
Bub1 Kinase and Sgo1 Are Required for Efficient Chromosome Biorientation following Nocodazole Treatment
We have demonstrated that both bub1ΔK and sgo1Δ mutants have a defect in chromosome biorientation upon nocodazole release (Figure 5). In many of the mitotic cells we observed chromosomes that fail to biorient, lying close to one of the SPBs. One explanation could be that these mutants fail to detect or respond to inappropriate attachments, for example syntelic attachments that lack tension. This hypothesis is supported by the fact that bub1ΔK and sgo1Δ cells lack the ability to delay anaphase onset in response to tension defects induced by reduced cohesion (Figure 2 and ). Alternatively, bub1ΔK and sgo1Δ cells may sense these defects but be unable to break the inappropriate kinetochore-microtubule attachments. The Yen lab have recently demonstrated that human Sgo2 is not required to recruit Passenger proteins to centromeres but is needed to recruit the kinesin MCAK . The activity of that kinesin, which is an Aurora B substrate, is known to be important for breaking inappropriate kinetochore attachments in some systems [61,62]. In addition, Sgo proteins have been shown to bind directly to microtubules and to modulate kinetochore-microtubule dynamics , so it is also possible that Sgo1p has a direct role in breaking microtubule attachments.
Bub1 kinase and Sgo1p could be part of the pathway that employs the Passenger proteins to destabilise kinetochore-microtubule attachments that lack tension. If so, one would expect similar phenotypes between bub1ΔK, sgo1Δ, and Passenger protein mutants. There are striking similarities: (1) ipl1 and sli15 mutants can arrest the cell cycle in response to unattached kinetochores because of nocodazole treatment, and (2) ipl1 mutants fail to delay anaphase onset when cohesion is reduced and there is a lack of tension at kinetochores [23,64]. However, the Passenger proteins are essential, and are required for response to “core” kinetochore attachment defects, such as those present in ndc80–1 and mtw1–1 . Figure 6 contains models that attempt to explain these observations.
In certain circumstances, such as those found during spindle assembly upon nocodoazole washout, the activity of Bub1 kinase and Sgo1p become necessary to ensure complete biorientation. Our data show that Bub1 kinase is required for efficient localisation of Sgo1p to centromeres, which in turn may aid efficient targeting of the Passenger proteins or their targets. Such a mode of action places Bub1p, Sgo1p and the Passengers in a simple linear pathway (Figure 6A). However, due to the lack of a clear effect on Passenger protein localisation, we currently favour an alternative model in which Bub1p, Sgo1p and the Passengers act cooperatively, but in distinct pathways, to ensure efficient biorientation (Figure 6B). The identification of direct Bub1 kinase substrates will be key to a deeper understanding of the role(s) of Bub1 kinase in the complex regulation of kinetochore attachment and error correction in mitosis.
Materials and Methods
Yeast strains, media, and standard techniques.
The yeast strains used in this study are derivatives of W303 (ade2–1 his3–11 leu2–112 trp1–1 can1–100 ssdl-d2) and are listed in Table 1. Yeast strains were grown in YPDA or selective media, and other basic yeast methods have been previously described. For synchronisation of cells in G1 we used 1 μg/ml α-factor for bar1 and 10 μg/ml α-factor BAR1 strains. Tagging of Spc42-Tomato (strains JF152, JF154, and JF156) was performed using cassettes described in [65,66].
Creation of bub1ΔK allele.
Amino acids 609–1,021 were truncated by PCR-mediated gene disruption, using pFA6a-Hygromycin resistance cassette  and verified by PCR and sequencing. The sgo1Δ strain was created using pFA6-kanMX6 .
Benomyl, microcolony, and viability assays were all performed as previously described [9,41,69]. The mtw1–1 checkpoint activation assay was performed as in . The depletion of cohesin (lack of tension) assay was performed using GAL-MCD1 as described .
A linear minichromosome assay was employed essentially as described in . The linear chromosome used (pA241) is marked with LEU2. Such chromosomes have been shown to delay mitosis in a checkpoint-dependent manner  and were employed in a screen that identified sgo1 alleles . In the strains used here, and for the screen, the linear chromosome-induced delay becomes lethal as the cells also contain CDC28-VF, a mutation in CDC28/CDK1 that reduces APC activity. This lethality is rescued by checkpoint mutations, as no mitotic delay is imposed. In addition, doxycycline represses a dominant CDC20 allele that is insensitive to the spindle checkpoint proteins because it contains a mutation in the Mad2p binding site . This means that all strains (wild-type or checkpoint mutant) containing the LMCs can be propagated in the absence of doxycycline. When doxycycline is added only wild-type CDC20 is expressed: if the strain is checkpoint proficient it will not form colonies.
Chromosome segregation assays.
Strains containing GFP-marked Chromosome IV was presynchronised in G1 and a sample was fixed in 3.7% formaldehyde for 5 min. The cells were then released into 30 μg/ml nocodazole and grown at 23 °C for 3 h. The nocodazole was then washed out, three times with YPDA media and then arrested in the next G1, and another sample was fixed as above. The same strain was used to analyse chromosome segregation at the first anaphase after nocodazole arrest. Cells were again presynchronised in G1 and arrested with nocodazole as above. Cells were released and fixed for 1 h in 3.7% formaldehyde and washed with 0.1 M potassium phosphate (pH 7.5). Then they were treated with 50 μg/ml Zymolyase 100,000 in 0.1 M potassium phosphate/0.7 M Sorbitol. General immunofluorescence was performed as previously described in . Quantitative chromosome mis-segregation assay (sectoring assay) was performed as previously described [30,47].
Chromosome spreads were performed on cycling cells and on nocodazole arrested cells as previously described by [70,71]. Sgo1-9myc was detected using rabbit anti-myc antibody (CM-100, Gramsch) at a 1:800 dilution, and anti-rabbit Alexa Fluor 488 (Invitrogen) at a 1:1,000 dilution. Ndc10-6HA was detected using a mouse anti-HA antibody (HA11, BabCO) at a 1:200 dilution and anti-mouse Alexa Fluor 594 (Invitrogen) at a 1:1,000 dilution. The spindle pole bodies were marked using an anti-Spc110 antibody at a 1:50 dilution (kind gift from John Kilmartin) and anti-mouse Alexa Fluor 594 (Invitrogen) at a 1:1,000 dilution.
Cells were arrested for 3 h in 15 μg/ml nocodazole and 30 μg/ml benomyl, and 50 ml cells were collected for ChIP. SGO1- 6HA ChIPs were performed using 12CA5 anti-HA antibody (Roche), and the general protocol including primers was performed as described in .
Chromosome attachment assay.
Similar experiments to those described in  were performed, where the mitotic spindle is labelled with tubulin-CFP and the kinetochores with Mtw1-3xGFP. Strains were treated with 15 μg/ml nocodazole and 30 μg/ml benomyl at 23 °C for 3 h. Cells were then washed three times and grown in YPDA at 30 °C for 20 min, then fixed in 3.7% formaldehyde for 5 min. ndc80–1 cells were grown for the last 90 min at the restrictive temperature of 36 °C.
Chromosome biorientation assay.
Strains were arrested in G1 in medium lacking methionine for 3 h, then transferred to YPDA media plus 8 mM methionine for 2 h at 30 °C to deplete Cdc20. The α-factor was subsequently washed out, and cells were then incubated at 23 °C for 3 h in media containing 30 μg/ml benomyl, 30 μg/ml nocodazole, and 8mM methionine. The microtubule drugs were then washed out, and the spindle was allowed to reform at 30 °C in YPDA media plus 8 mM methionine. Cells were fixed at indicated times in 3.7% formaldehyde for 5 min. The GFP dots were analysed only in cells that had a short bipolar spindle (i.e., two SPBs) to score for biorientation. These experiments were repeated at least three times, and at each time point 100 cells were counted.
Figure S1. bub1ΔK Cells Initiate and Maintain a Robust Checkpoint Arrest in Response to Antimicrotubule Drugs
(A) Wild-type (KH186), bub1ΔK (JF098), bub1Δ (KH127), and mad2Δ (KH141) yeast strains were analysed for growth on YPDA media containing (A) 20 μg/ml or (B) 80 μg/ml benomyl grown at 23 °C. We scored the percentage of cells that remain arrested (large-budded) throughout the time course (n = 50 cells). The bub1ΔK cells initiate and maintain a robust checkpoint arrest.
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Figure S2. Sgo1 Localisation to Kinetochores (Ndc10) Is Defective in bub1ΔK
(A) Wild-type (AMY1110), bub1ΔK (JF038), and bub1Δ (AMY1379) cells were harvested in log-phase, and staining was performed on chromosome spreads. Anti-myc (CM-100) was used to detect Sgo1-9myc, and anti-HA (HA11) was used to detect Ndc10-6HA. Cells with clear Ndc10-6HA staining were categorised as either having colocalisation with Sgo1-9myc (left panel), or only partial colocalisation that could be due to spindle pole bodies (right panel). The spreads with no Sgo1 staining are not shown but were similar in number in wild type and bub1ΔK mutant.
(B) Quantification of spreads scoring percentage of spreads that showed colocalisation of Sgo1 to the Ndc10 kinetochore marker. Error bars indicate standard deviation of the mean.
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Figure S3. Mcd1 Cleavage Is Not Defective in bub1ΔK Cells
Wild-type (AMY1145) and bub1ΔK (JF216) strains were arrested in mitosis using 15 μg/ml nocodazole and 30 μg/ml benomyl at 23 °C for 3 h. The drug was washed out and samples for immunoblotting with 12CA5 (anti-HA antibody) were taken at indicated timepoints. There was no significant difference in timing or amount of Mcd1 cleavage detected. Blot shows a representative experiment. The experiment was repeated three times.
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Figure S4. Kinetochore Localisation of Ipl1 Kinase Is Not Significantly Perturbed in bub1ΔK Cells
Wild-type (T5241) and bub1ΔK (JF169) strains were arrested in mitosis using 15 μg/ml nocodazole and 30 μg/ml benomyl. Kinetochores were marked using Ctf19-CFP and Ndc80-CFP. There was no significant reduction of Ipl1-GFP on kinetochores in the bub1ΔK strain compared to wild-type cells.
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We are extremely grateful to Adèle Marston for a great deal of help and advice, in particular with chromosome spreads and ChIP analyses, and to Andrew Murray for communicating data prior to publication. In addition, we would like to thank Adèle Marston, Andrew Murray, Sue Biggins, Mike Stark, and John Kilmartin for yeast strains, reagents, and helpful discussions, and Ken Sawin for fluorescent tagging constructs.
JF and KGH conceived and designed the experiments, analysed the data, and wrote the paper. JF performed the experiments.
- 1. Tanaka TU, Stark MJ, Tanaka K (2005) Kinetochore capture and bi-orientation on the mitotic spindle. Nat Rev Mol Cell Biol 6: 929–942.TU TanakaMJ StarkK. Tanaka2005Kinetochore capture and bi-orientation on the mitotic spindle.Nat Rev Mol Cell Biol6929942
- 2. Maiato H, DeLuca J, Salmon ED, Earnshaw WC (2004) The dynamic kinetochore-microtubule interface. J Cell Sci 117: 5461–5477.H. MaiatoJ. DeLucaED SalmonWC Earnshaw2004The dynamic kinetochore-microtubule interface.J Cell Sci11754615477
- 3. Cleveland DW, Mao Y, Sullivan KF (2003) Centromeres and kinetochores. From epigenetics to mitotic checkpoint signaling. Cell 112: 407–421.DW ClevelandY. MaoKF Sullivan2003Centromeres and kinetochores. From epigenetics to mitotic checkpoint signaling.Cell112407421
- 4. Kops GJ, Weaver BA, Cleveland DW (2005) On the road to cancer: aneuploidy and the mitotic checkpoint. Nat Rev Cancer 5: 773–785.GJ KopsBA WeaverDW Cleveland2005On the road to cancer: aneuploidy and the mitotic checkpoint.Nat Rev Cancer5773785
- 5. Hassold T, Hunt P (2001) To err (meiotically) is human: the genesis of human aneuploidy. Nat Rev Genet 2: 280–291.T. HassoldP. Hunt2001To err (meiotically) is human: the genesis of human aneuploidy.Nat Rev Genet2280291
- 6. Musacchio A, Salmon ED (2007) The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol 8: 379–393.A. MusacchioED Salmon2007The spindle-assembly checkpoint in space and time.Nat Rev Mol Cell Biol8379393
- 7. Taylor SS, Scott MI, Holland AJ (2004) The spindle checkpoint: a quality control mechanism which ensures accurate chromosome segregation. Chromosome Res 12: 599–616.SS TaylorMI ScottAJ Holland2004The spindle checkpoint: a quality control mechanism which ensures accurate chromosome segregation.Chromosome Res12599616
- 8. Hoyt MA, Totis L, Roberts BT (1991) S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell 66: 507–517.MA HoytL. TotisBT Roberts1991S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function.Cell66507517
- 9. Li R, Murray AW (1991) Feedback control of mitosis in budding yeast. Cell 66: 519–531.R. LiAW Murray1991Feedback control of mitosis in budding yeast.Cell66519531
- 10. Weiss E, Winey M (1996) The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint. J Cell Biol 132: 111–123.E. WeissM. Winey1996The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint.J Cell Biol132111123
- 11. Chen RH, Shevchenko A, Mann M, Murray AW (1998) Spindle checkpoint protein Xmad1 recruits xmad2 to unattached kinetochores. J Cell Biol 143: 283–295.RH ChenA. ShevchenkoM. MannAW Murray1998Spindle checkpoint protein Xmad1 recruits xmad2 to unattached kinetochores.J Cell Biol143283295
- 12. Vigneron S, Prieto S, Bernis C, Labbe JC, Castro A, et al. (2004) Kinetochore localization of spindle checkpoint proteins: who controls whom? Mol Biol Cell 15: 4584–4596.S. VigneronS. PrietoC. BernisJC LabbeA. Castro2004Kinetochore localization of spindle checkpoint proteins: who controls whom?Mol Biol Cell1545844596
- 13. Hwang LH, Lau LF, Smith DL, Mistrot CA, Hardwick KG, et al. (1998) Budding yeast Cdc20: a target of the spindle checkpoint. Science 279: 1041–1044.LH HwangLF LauDL SmithCA MistrotKG Hardwick1998Budding yeast Cdc20: a target of the spindle checkpoint.Science27910411044
- 14. Kim SH, Lin DP, Matsumoto S, Kitazono A, Matsumoto T (1998) Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint. Science 279: 1045–1047.SH KimDP LinS. MatsumotoA. KitazonoT. Matsumoto1998Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint.Science27910451047
- 15. Peters JM (2006) The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat Rev Mol Cell Biol 7: 644–656.JM Peters2006The anaphase promoting complex/cyclosome: a machine designed to destroy.Nat Rev Mol Cell Biol7644656
- 16. Cohen-Fix O (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10: 3081–3093.O. Cohen-Fix1996Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.Genes Dev1030813093
- 17. Shirayama M, Toth A, Galova M, Nasmyth K (1999) APC(Cdc20) promotes exit from mitosis by destroying the anaphase inhibitor Pds1 and cyclin Clb5. Nature 402: 203–207.M. ShirayamaA. TothM. GalovaK. Nasmyth1999APC(Cdc20) promotes exit from mitosis by destroying the anaphase inhibitor Pds1 and cyclin Clb5.Nature402203207
- 18. Thornton BR, Toczyski DP (2003) Securin and B-cyclin/CDK are the only essential targets of the APC. Nat Cell Biol 5: 1090–1094.BR ThorntonDP Toczyski2003Securin and B-cyclin/CDK are the only essential targets of the APC.Nat Cell Biol510901094
- 19. Rieder CL, Cole RW, Khodjakov A, Sluder G (1995) The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores. J Cell Biol 130: 941–948.CL RiederRW ColeA. KhodjakovG. Sluder1995The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores.J Cell Biol130941948
- 20. Waters JC, Chen RH, Murray AW, Salmon ED (1998) Localization of Mad2 to kinetochores depends on microtubule attachment, not tension. J Cell Biol 141: 1181–1191.JC WatersRH ChenAW MurrayED Salmon1998Localization of Mad2 to kinetochores depends on microtubule attachment, not tension.J Cell Biol14111811191
- 21. Li X, Nicklas RB (1995) Mitotic forces control a cell-cycle checkpoint. Nature 373: 630–632.X. LiRB Nicklas1995Mitotic forces control a cell-cycle checkpoint.Nature373630632
- 22. Pinsky BA, Biggins S (2005) The spindle checkpoint: tension versus attachment. Trends Cell Biol 15: 486–493.BA PinskyS. Biggins2005The spindle checkpoint: tension versus attachment.Trends Cell Biol15486493
- 23. Biggins S, Murray AW (2001) The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the spindle checkpoint. Genes Dev 15: 3118–3129.S. BigginsAW Murray2001The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the spindle checkpoint.Genes Dev1531183129
- 24. Pinsky BA, Kung C, Shokat KM, Biggins S (2006) The Ipl1-Aurora protein kinase activates the spindle checkpoint by creating unattached kinetochores. Nat Cell Biol 8: 78–83.BA PinskyC. KungKM ShokatS. Biggins2006The Ipl1-Aurora protein kinase activates the spindle checkpoint by creating unattached kinetochores.Nat Cell Biol87883
- 25. King EM, Rachidi N, Morrice N, Hardwick KG, Stark MJ (2007) Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores. Genes Dev 21: 1163–1168.EM KingN. RachidiN. MorriceKG HardwickMJ Stark2007Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.Genes Dev2111631168
- 26. Roberts BT, Farr KA, Hoyt MA (1994) The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol Cell Biol 14: 8282–8291.BT RobertsKA FarrMA Hoyt1994The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase.Mol Cell Biol1482828291
- 27. Brady DM, Hardwick KG (2000) Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function. Curr Biol 10: 675–678.DM BradyKG Hardwick2000Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function.Curr Biol10675678
- 28. Johnson VL, Scott MI, Holt SV, Hussein D, Taylor SS (2004) Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression. J Cell Sci 117: 1577–1589.VL JohnsonMI ScottSV HoltD. HusseinSS Taylor2004Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression.J Cell Sci11715771589
- 29. Vanoosthuyse V, Valsdottir R, Javerzat JP, Hardwick KG (2004) Kinetochore targeting of fission yeast Mad and Bub proteins is essential for spindle checkpoint function but not for all chromosome segregation roles of Bub1p. Mol Cell Biol 24: 9786–9801.V. VanoosthuyseR. ValsdottirJP JaverzatKG Hardwick2004Kinetochore targeting of fission yeast Mad and Bub proteins is essential for spindle checkpoint function but not for all chromosome segregation roles of Bub1p.Mol Cell Biol2497869801
- 30. Warren CD, Brady DM, Johnston RC, Hanna JS, Hardwick KG, et al. (2002) Distinct chromosome segregation roles for spindle checkpoint proteins. Mol Biol Cell 13: 3029–3041.CD WarrenDM BradyRC JohnstonJS HannaKG Hardwick2002Distinct chromosome segregation roles for spindle checkpoint proteins.Mol Biol Cell1330293041
- 31. Tang Z, Shu H, Oncel D, Chen S, Yu H (2004) Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol Cell 16: 387–397.Z. TangH. ShuD. OncelS. ChenH. Yu2004Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint.Mol Cell16387397
- 32. Bernard P, Hardwick K, Javerzat JP (1998) Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis. J Cell Biol 143: 1775–1787.P. BernardK. HardwickJP Javerzat1998Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis.J Cell Biol14317751787
- 33. Meraldi P, Sorger PK (2005) A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J 24: 1621–1633.P. MeraldiPK Sorger2005A dual role for Bub1 in the spindle checkpoint and chromosome congression.EMBO J2416211633
- 34. Watanabe Y (2005) Shugoshin: guardian spirit at the centromere. Curr Opin Cell Biol 17: 590–595.Y. Watanabe2005Shugoshin: guardian spirit at the centromere.Curr Opin Cell Biol17590595
- 35. Katis VL, Galova M, Rabitsch KP, Gregan J, Nasmyth K (2004) Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332. Curr Biol 14: 560–572.VL KatisM. GalovaKP RabitschJ. GreganK. Nasmyth2004Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332.Curr Biol14560572
- 36. Kitajima TS, Kawashima SA, Watanabe Y (2004) The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis. Nature 427: 510–517.TS KitajimaSA KawashimaY. Watanabe2004The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis.Nature427510517
- 37. Marston AL, Tham WH, Shah H, Amon A (2004) A genome-wide screen identifies genes required for centromeric cohesion. Science 303: 1367–1370.AL MarstonWH ThamH. ShahA. Amon2004A genome-wide screen identifies genes required for centromeric cohesion.Science30313671370
- 38. Rabitsch KP, Gregan J, Schleiffer A, Javerzat JP, Eisenhaber F, et al. (2004) Two fission yeast homologs of Drosophila Mei-S332 are required for chromosome segregation during meiosis I and II. Curr Biol 14: 287–301.KP RabitschJ. GreganA. SchleifferJP JaverzatF. Eisenhaber2004Two fission yeast homologs of Drosophila Mei-S332 are required for chromosome segregation during meiosis I and II.Curr Biol14287301
- 39. Indjeian VB, Stern BM, Murray AW (2005) The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes. Science 307: 130–133.VB IndjeianBM SternAW Murray2005The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes.Science307130133
- 40. Yamaguchi S, Decottignies A, Nurse P (2003) Function of Cdc2p-dependent Bub1p phosphorylation and Bub1p kinase activity in the mitotic and meiotic spindle checkpoint. EMBO J 22: 1075–1087.S. YamaguchiA. DecottigniesP. Nurse2003Function of Cdc2p-dependent Bub1p phosphorylation and Bub1p kinase activity in the mitotic and meiotic spindle checkpoint.EMBO J2210751087
- 41. Straight AF, Belmont AS, Robinett CC, Murray AW (1996) GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion. Curr Biol 6: 1599–1608.AF StraightAS BelmontCC RobinettAW Murray1996GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion.Curr Biol615991608
- 42. Wells WAE, Murray AW (1996) Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast. J Cell Biol 133: 75–84.WAE WellsAW Murray1996Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast.J Cell Biol1337584
- 43. Chan CS, Botstein D (1993) Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast. Genetics 135: 677–691.CS ChanD. Botstein1993Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast.Genetics135677691
- 44. Riedel CG, Katis VL, Katou Y, Mori S, Itoh T, et al. (2006) Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I. Nature 441: 53–61.CG RiedelVL KatisY. KatouS. MoriT. Itoh2006Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.Nature4415361
- 45. Kitajima TS, Hauf S, Ohsugi M, Yamamoto T, Watanabe Y (2005) Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization. Curr Biol 15: 353–359.TS KitajimaS. HaufM. OhsugiT. YamamotoY. Watanabe2005Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization.Curr Biol15353359
- 46. Tang Z, Sun Y, Harley SE, Zou H, Yu H (2004) Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis. Proc Natl Acad Sci U S A 101: 18012–18017.Z. TangY. SunSE HarleyH. ZouH. Yu2004Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis.Proc Natl Acad Sci U S A1011801218017
- 47. Hieter P, Mann C, Snyder M, Davis RW (1985) Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss. Cell 40: 381–392.P. HieterC. MannM. SnyderRW Davis1985Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss.Cell40381392
- 48. Wigge PA, Kilmartin JV (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152: 349–360.PA WiggeJV Kilmartin2001The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation.J Cell Biol152349360
- 49. Goshima G, Yanagida M (2000) Establishing biorientation occurs with precocious separation of the sister kinetochores, but not the arms, in the early spindle of budding yeast. Cell 100: 619–633.G. GoshimaM. Yanagida2000Establishing biorientation occurs with precocious separation of the sister kinetochores, but not the arms, in the early spindle of budding yeast.Cell100619633
- 50. He X, Asthana S, Sorger PK (2000) Transient sister chromatid separation and elastic deformation of chromosomes during mitosis in budding yeast. Cell 101: 763–775.X. HeS. AsthanaPK Sorger2000Transient sister chromatid separation and elastic deformation of chromosomes during mitosis in budding yeast.Cell101763775
- 51. Tanaka TU, Rachidi N, Janke C, Pereira G, Galova M, et al. (2002) Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections. Cell 108: 317–329.TU TanakaN. RachidiC. JankeG. PereiraM. Galova2002Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections.Cell108317329
- 52. Indjeian VB, Murray AW (2007) Budding yeast mitotic chromosomes have an intrinsic bias to bi-orient on the spindle. Curr Biol 17: 1837–1846.VB IndjeianAW Murray2007Budding yeast mitotic chromosomes have an intrinsic bias to bi-orient on the spindle.Curr Biol1718371846
- 53. Hardwick KG, Weiss E, Luca FC, Winey M, Murray AW (1996) Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption. Science 273: 953–956.KG HardwickE. WeissFC LucaM. WineyAW Murray1996Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption.Science273953956
- 54. Vagnarelli P, Earnshaw WC (2004) Chromosomal passengers: the four-dimensional regulation of mitotic events. Chromosoma 113: 211–222.P. VagnarelliWC Earnshaw2004Chromosomal passengers: the four-dimensional regulation of mitotic events.Chromosoma113211222
- 55. Resnick TD, Satinover DL, MacIsaac F, Stukenberg PT, Earnshaw WC, et al. (2006) INCENP and Aurora B promote meiotic sister chromatid cohesion through localization of the Shugoshin MEI-S332 in Drosophila. Dev Cell 11: 57–68.TD ResnickDL SatinoverF. MacIsaacPT StukenbergWC Earnshaw2006INCENP and Aurora B promote meiotic sister chromatid cohesion through localization of the Shugoshin MEI-S332 in Drosophila.Dev Cell115768
- 56. Monje-Casas F, Prabhu VR, Lee BH, Boselli M, Amon A (2007) Kinetochore orientation during meiosis is controlled by Aurora B and the monopolin complex. Cell 128: 477–490.F. Monje-CasasVR PrabhuBH LeeM. BoselliA. Amon2007Kinetochore orientation during meiosis is controlled by Aurora B and the monopolin complex.Cell128477490
- 57. Yu HG, Koshland D (2007) The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis. J Cell Biol 176: 911–918.HG YuD. Koshland2007The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.J Cell Biol176911918
- 58. Kawashima SA, Tsukahara T, Langegger M, Hauf S, Kitajima TS, et al. (2007) Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres. Genes Dev 21: 420–435.SA KawashimaT. TsukaharaM. LangeggerS. HaufTS Kitajima2007Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres.Genes Dev21420435
- 59. Vanoosthuyse V, Prykhozhij S, Hardwick KG (2007) Shugoshin 2 regulates localization of the chromosomal passenger proteins in fission yeast mitosis. Mol Biol Cell 18: 1657–1669.V. VanoosthuyseS. PrykhozhijKG Hardwick2007Shugoshin 2 regulates localization of the chromosomal passenger proteins in fission yeast mitosis.Mol Biol Cell1816571669
- 60. Huang H, Feng J, Famulski J, Rattner JB, Liu ST, et al. (2007) Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments. J Cell Biol 177: 413–424.H. HuangJ. FengJ. FamulskiJB RattnerST Liu2007Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments.J Cell Biol177413424
- 61. Andrews PD, Ovechkina Y, Morrice N, Wagenbach M, Duncan K, et al. (2004) Aurora B regulates MCAK at the mitotic centromere. Dev Cell 6: 253–268.PD AndrewsY. OvechkinaN. MorriceM. WagenbachK. Duncan2004Aurora B regulates MCAK at the mitotic centromere.Dev Cell6253268
- 62. Lan W, Zhang X, Kline-Smith SL, Rosasco SE, Barrett-Wilt GA, et al. (2004) Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity. Curr Biol 14: 273–286.W. LanX. ZhangSL Kline-SmithSE RosascoGA Barrett-Wilt2004Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity.Curr Biol14273286
- 63. Salic A, Waters JC, Mitchison TJ (2004) Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis. Cell 118: 567–578.A. SalicJC WatersTJ Mitchison2004Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis.Cell118567578
- 64. Sandall S, Severin F, McLeod IX, Yates JR 3rd, Oegema K, et al. (2006) A Bir1-Sli15 complex connects centromeres to microtubules and is required to sense kinetochore tension. Cell 127: 1179–1191.S. SandallF. SeverinIX McLeodJR Yates 3rdK. Oegema2006A Bir1-Sli15 complex connects centromeres to microtubules and is required to sense kinetochore tension.Cell12711791191
- 65. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, et al. (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22: 1567–1572.NC ShanerRE CampbellPA SteinbachBN GiepmansAE Palmer2004Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.Nat Biotechnol2215671572
- 66. Snaith HA, Samejima I, Sawin KE (2005) Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast. EMBO J 24: 3690–3699.HA SnaithI. SamejimaKE Sawin2005Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast.EMBO J2436903699
- 67. Goldstein AL, McCusker JH (1999) Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15: 1541–1553.AL GoldsteinJH McCusker1999Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.Yeast1515411553
- 68. Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, et al. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953–961.MS LongtineA. McKenzie 3rdDJ DemariniNG ShahA. Wach1998Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae.Yeast14953961
- 69. Hardwick KG, Murray AW (1995) Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast. J Cell Biol 131: 709–720.KG HardwickAW Murray1995Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast.J Cell Biol131709720
- 70. Loidl J, Klein F, Engebrecht J (1998) Genetic and morphological approaches for the analysis of meiotic chromosomes in yeast. Methods Cell Biol 53: 257–285.J. LoidlF. KleinJ. Engebrecht1998Genetic and morphological approaches for the analysis of meiotic chromosomes in yeast.Methods Cell Biol53257285
- 71. Nairz K, Klein F (1997) mre11S–a yeast mutation that blocks double-strand-break processing and permits nonhomologous synapsis in meiosis. Genes Dev 11: 2272–2290.K. NairzF. Klein1997mre11S–a yeast mutation that blocks double-strand-break processing and permits nonhomologous synapsis in meiosis.Genes Dev1122722290
- 72. Kiburz BM, Reynolds DB, Megee PC, Marston AL, Lee BH, et al. (2005) The core centromere and Sgo1 establish a 50-kb cohesin-protected domain around centromeres during meiosis I. Genes Dev 19: 3017–3030.BM KiburzDB ReynoldsPC MegeeAL MarstonBH Lee2005The core centromere and Sgo1 establish a 50-kb cohesin-protected domain around centromeres during meiosis I.Genes Dev1930173030