Lifespan can be extended by reduction of dietary intake. This practice is referred to as dietary restriction (DR), and extension of lifespan by DR is evolutionarily conserved in taxonomically diverse organisms including yeast, invertebrates, and mammals. Although these two often-stated facts carry the implication that the mechanisms of DR are also evolutionarily conserved, extension of lifespan could be a case of evolutionary convergence, with different underlying mechanisms in different taxa. Furthermore, extension of lifespan by different methods of DR in the same organism may operate through different mechanisms. These topics remain unresolved because of the very fact that the mechanisms of DR are unknown. Given these uncertainties, it is essential that work on the mechanisms of DR is not clouded by imprecise descriptions of methods or by technical problems. Here we review the recent literature on DR in Drosophila to point out some methodological issues that can obscure mechanistic interpretations. We also indicate some experiments that could be performed to determine if DR in Drosophila operates through similar mechanisms to the process in rodents.
Citation: Piper MDW, Partridge L (2007) Dietary Restriction in Drosophila: Delayed Aging or Experimental Artefact? PLoS Genet 3(4): e57. https://doi.org/10.1371/journal.pgen.0030057
Editor: Nicholas Katsanis, Johns Hopkins University School of Medicine, United States of America
Published: April 27, 2007
Copyright: © 2007 Piper and Partridge. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: We acknowledge funding from the Wellcome Trust Functional Genomic Analysis of Ageing Grant (MP and LP) and the Biotechnology and Biological Science Research Council (LP).
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: DR, dietary restriction
At the beginning of the 20th century, the first experiments studying the effects of environmental interventions on lifespan were undertaken using the fruit fly Drosophila melanogaster . Of particular note is the work of Loeb and Northrop , which found that Drosophila maintained at lower temperatures were longer lived. Since metabolic rate in poikilotherms is determined by ambient temperature, it was proposed by Raymond Pearl that organisms have a genetically predetermined amount of energy to expend in a lifetime and, therefore, that the rate of its expenditure would determine lifespan [3,4]. The central mechanism of this “rate of living” hypothesis is that aging and subsequent death are brought about by an accumulation of the detrimental effects of the products of normal metabolism that cannot be adequately cleared.
At about the same time, Stefan Kopeć , working on the causes of death by starvation, reached the conclusion that it was brought about by “autointoxication” from products of hunger metabolism. In addition, he proposed that starvation provided “antitoxins” against those detrimental effects of normal metabolism referred to by Pearl above and, likewise, that normal feeding provided “antitoxins” against starvation. He thus reasoned that balancing these effects might benefit the organism and lead to prolonged lifespan. He tested this hypothesis experimentally in 1928 using Drosophila maintained on a regime of alternating periods of feeding and fasting . Interestingly, Kopeć reported that the “…results pointed undoubtedly to the conclusion that periods of intermittent starvation for six hours out of every 24 increases longevity of the flies . . . ” . However, examination of the data in the paper shows the effect was less than convincing, ranging from 16% shortening of mean lifespan to 17% extension, with an average effect over ten trials of 2% extension.
Seven years later, Clive McCay reported the effects of an intermittent feeding regime on the lifespan of white rats . This resulted in males achieving almost double the lifespan of those fed ad libitum, an effect that has since been repeated in rodents many times using a variety of different methods, and now termed caloric restriction or dietary restriction (DR) . We use the more general term “DR” in this review, because it covers both cases where total caloric intake correlated with extended lifespan and those where specific nutrients are of importance, leaving open which of these mechanisms is operative. Despite the initial lack of success that Kopeć  in fact had with the intermittent feeding DR protocol in flies, it has since been shown that it is possible, with appropriate techniques, to extend the lifespan of many organisms, including Drosophila, by reducing food intake (see Table S1 for a summary of DR experiments with flies and the range of techniques implemented). This has opened the way for the use of short-lived, invertebrate model organisms such as yeast, nematodes, and flies to investigate the mechanisms of DR that could also be operative in mammals.
A potential problem of comparative DR studies using distantly related species is that the mechanisms underlying the prolongation of life may not be conserved between different lineages during evolution, because the mechanisms by which DR extends lifespan within each species are unknown. To make progress with resolving the issue of evolutionary conservation, it is essential that studies of DR are reported in sufficient detail for the procedures to be replicated and that any intervention described as “DR” does in fact operate through a reduction in the supply of nutrients, rather than through the removal of some other detrimental effect. Despite the simplicity of this point, it is overlooked surprisingly frequently. Here, we consider how such technical issues could have affected conclusions of studies of DR in Drosophila. Resolving these issues are of central importance for uncovering the mechanisms of DR through comparative model organism studies.
Restricting Access to Nutrients
The most practical method for implementing DR in Drosophila is by dilution of the food medium to which the flies have ad libitum access . In addition to the work by Kopeć  cited above, several other laboratories have unsuccessfully attempted to implement DR in a variety of fly species using intermittent feeding regimes [9–12] (Table S1). This lack of success has been interpreted by some to indicate that DR does not work in flies [9,12,13]. An alternative explanation is that there is a detrimental effect of daily periods of extended starvation for flies that is not found for rodents when fed intermittently. This detrimental effect could counter-act any beneficial effects of underfeeding and so fail to extend lifespan. This could occur if the intermittently fed animals have specific nutritional needs during the starvation period that must be met for lifespan to be extended by intermittent feeding. Interestingly, Partridge et al.  found that when they intermittently fed yeast to flies (every sixth day) that had constant access to sugar-water, lifespan was extended by some 30% compared with flies fed yeast and sugar ad libitum. This contrasts with the negative results from other intermittent feeding studies, where the flies had constant access to water that did not contain sucrose [5,12]. These data indicate that a carbohydrate-enriched diet may be necessary to rescue a detrimental effect of starvation periods for Drosophila. The result also points to the specific nutritional effects of yeast being important for lifespan-extension by DR in flies, which has recently been confirmed .
Food Dilution—Toxicity versus Nutrient-Dependent Lifespan Extension
As mentioned above, DR in flies can be achieved by diluting the concentration of nutrients in their food medium, which is always present in excess (Table S1). One food type for Drosophila consists of an agar-gelled diet of dried autolysed yeast and sucrose [16,17]. The effect of changing the concentrations of these nutrients ranges from outright starvation at the lowest food levels, through a lifespan peak (DR), to a decrease in lifespan as nutrient concentration becomes “high” (Figure 1). The increase in lifespan seen from “high” levels of nutrition to DR is generally interpreted as a consequence of a positive effect of withdrawal of nutrients on the systems that ensure longer life, perhaps via a reduction in activities such as reproduction that may cause somatic damage . A risk of using food dilution to implement DR is the possibility that flies compensate for lowered nutrient levels by increasing their feeding behaviour. At this stage, both evidence for  and against [15,20] compensatory feeding has been published. This discrepancy needs to be addressed with further work and may simply be due to the technical difficulties in determining the feeding rate of such a small organism (see Video S1 for an example of Drosophila feeding). It is estimated that Drosophila only consume between one and two microlitres of food per 24 hour period [19,21], 40-fold less than a blowfly for the same period of time . Despite these difficulties, it is clear that egg-laying output, which is known to be under nutrient control [23–25], increases as food concentration increases [17,26]. Thus, if compensatory feeding does occur, it is not sufficient to overcome the degree of nutrient dilution in the diet, and the lifespan increase seen under DR correlates with a decrease in acquisition of biologically useful nutrients. However, this correlation does not reveal causation and an alternative explanation is that reduced food supply simply relieves “high”-food flies from a non-nutritional, toxic effect of the diet.
As food concentration increases from starvation, lifespan should increase to a peak at DR, from which it declines due to a nutrient-dependent effect of “high” food. If fecundity decreases (A) or is unchanged (B) by the nutrient increase that decreases lifespan, the most likely explanation for shortened lifespan is toxicity. To minimise the possibility that food toxicity is the explanation for the lifespan shortening at high food concentrations, it is important that daily and lifetime fecundity increase for the increases in food concentrations that decrease lifespan (C).
Dietary toxicity could occur from either the presence of a poisonous element in the food, or via an indirect effect of the food being nutritionally inadequate or unbalanced, or physically dangerous, or a source of infection. Whatever the precise mechanism, if toxicity increases as the food concentration increases, the effect is indistinguishable from DR, since lifespan is shortened as food concentration increases. For Drosophila, where the food is the nutrient and water supply as well as a large part of the physical environment, increasing the nutritional value of the food also results in an increase in the ratio of dissolved nutrients to water. This could therefore cause flies to suffer shortened lifespan due to increased food hardness or water stress. We have recently tested this possibility and shown that when experiments are carefully performed, neither food hardness nor water shortage account for the life-shortening effects of high-nutrient food . It has also been suggested that increased nutrient content may enhance microbial growth, which could shorten fly lifespan by making the food more sticky or by encouraging a greater chance of lethal infection. Where these two factors have been tested, they have been shown not to affect lifespan [15,26]. For C. elegans, recent work has indicated that its commonly used laboratory feedstock, E. coli, has a toxic effect that shortens worm lifespan. In addition to worms surviving longer on killed E. coli media or in its complete absence [28–31], when worms were fed the soil bacterium B. subtilis instead of E. coli they were much longer lived [32,33]. This means that studies of “DR” in worms that rely on dilution of an E. coli food supply report the combined effects of reduced toxicity as well as reduced nutrient supply. In a more general sense, any natural food supply could contain toxins as well as nutrients, and therefore it is important to establish that any “DR” effect is in fact due to reduced intake of nutrients and not any direct or indirect toxicity effect of the food.
For flies, the problem of food toxicity is to some extent countered by using female egg-laying as a biologically relevant read-out of nutritional quality. If egg-laying increases with food supply, then it is reasonable to deduce that nutrient intake is increased. In combination with lifespan, egg-laying can indicate if food toxicity might be the cause of lifespan shortening, the argument being that if a fly does not increase its egg-laying for nutrient level increases that decrease lifespan, the food may be having a general toxic effect. For DR therefore, each increase in nutrient concentration that leads to a reduction in lifespan should be accompanied by an elevation in daily and lifetime fecundity (Figure 1). At the very least, this ensures that dietary types that are used for DR do result in increased nutrition over the range tested. A further test for food toxicity could be made using behaviour assays such as negative geotaxis  on young flies. Since the quality of industrially produced yeasts is dependent on the production method and seasonal quality of the feedstock, it is important that laboratories empirically determine whether they are working with yeast that is not toxic to flies. Unfortunately, not all studies have taken this precaution (Table S1). For instance, some work on the effects of dietary lipids on lifespan was performed without any simultaneous measure of egg laying or activity, thus making it impossible to know if increased food supply was in fact associated with increased nutrition, or if the short lifespans associated with elevated lipid supply were due to a nutritional effect or instead due to toxicity of the lipid sources added [35–37]. In some cases, such as experiments with males or sterile females, egg laying cannot be used to indicate the food quality. In these instances, informed choices can be made using data from fertile females of the same genetic stock or, as mentioned above, by the use of behavioural assays such as climbing ability. A biological indicator of nutrient quality other than lifespan is essential, because inter-species comparisons of the mechanisms of DR rely on the fact that DR is actually being studied in each organism.
One important line of work to uncover the mechanisms of DR has focussed on isolating the specific nutritional requirements for the effect on lifespan. The community working on DR in rodents has generally taken the view that reduced calorie intake is critical for the lifespan-extension associated with food reduction and has therefore adopted the term caloric restriction [7,38]. However, it is also acknowledged that other types of food restriction that do not affect calorie intake can also extend lifespan [7,39–46]. Each of these interventions may or may not lead to lifespan-extension through the same mechanisms. For instance, dietary restriction for a particular nutrient such as methionine [42,45] may in fact affect the utilization of other dietary components such that the organism ultimately experiences physiological energy restriction. Studies of the effect of exposure to diets of varying composition on food intake, and of the effects diet composition has on absorption of specific nutrients are needed to resolve these issues. In addition to these effects, there is evidence in worms and flies that olfaction of food, as well as food intake, can shorten lifespan [47,48]. One clear fact to emerge from the body of work on rodents is that, in addition to the data implicating reduced calorie intake as sufficient for lifespan extension, changes to either the quality or quantity of the protein component of the diet has the capacity to alter lifespan, even when caloric intake is equivalent between cohorts [39,40,44,45,49]. Recently, work on Drosophila has reached a similar conclusion, by showing that specific reduction of the yeast component in an otherwise isocaloric diet can extend lifespan to a similar degree as whole-food (yeast and sucrose) reduction, which also has lower caloric content . Even more recently, an attempt has been made to use semi-defined media to study DR in flies . This paper concluded that the protein component of the diet was critical for the effect. However, the flies on the semi-defined diet had dramatically reduced daily and lifetime fecundity and were not longer-lived than fully fed controls, indicating a toxic effect of the protein source (casein) being used. Thus it is important that future work focuses on developing appropriate semi- and fully-defined diets that avoid complications from toxicity.
An attractive candidate mechanism for the prolongation of life in response to food reduction, and more specifically protein shortage, is the activation of autophagy [51–53]. This process turns over subcellular material in response to low nutrients and is as evolutionarily conserved as the DR effect itself . Autophagy is activated by reduced insulin and TOR (Target Of Rapamycin) signaling and is therefore enhanced when both the energy and amino acid status of the organism are lowered [55–57]. Autophagy acts to recycle damaged/malfunctioning proteins and organelles from the cell and may, therefore, as a by-product of this action, clear out damaged macromolecules that contribute to aging. While there is currently no direct evidence that increased autophagy in older individuals can delay aging, it has been shown in C. elegans that autophagy is required for the lifespan extension of worms mutant for the insulin receptor daf-2 . In addition, a rodent study reporting the effects of every-other-day feeding on lifespan [41,43] is also compatible with an autophagy-based mechanism. In this experiment, rodents were dietarily restricted by allowing them to feed ad libitum during restricted hours. During the periods of feeding, the restricted cohort consumed almost the same quantity as rodents fed ad libitum all the time. These “restricted” animals were long-lived despite consuming almost the same diet as those fed ad libitum and achieved a very similar body weight. Thus, for this intervention at least, the advantage gained by the long-lived animals was not due to reduced calorie or food intake, but was acquired by periods of fasting—a concept remarkably similar to Kopeć's “antitoxins” furnished by periods of starvation , and compatible with an autophagy-like process acting to recycle cellular material to scavenge resources. Clearly, there is a need for further work to determine if an organism's response to DR is autophagy-dependent and if maintaining autophagy in an active state for longer can prolong lifespan.
The Consequences of Sex during DR
One of the long-known features of DR animals is that they have compromised reproductive capacity. Indeed, this feature of DR has been used to form hypotheses about the evolutionary significance of the response to DR, based on the concept of allocation of limiting resources between somatic maintenance and reproduction [59–62]. For many organisms, it could be beneficial during lean times to increase allocation of nutritional resources into maintaining the adult, to increase the chance of survival to a time when the food supply is restored. In Drosophila, high levels of nutrition increase female egg production. This leads them to use up their supplies of stored sperm more rapidly  and to re-mate more frequently than those with poorer nourishment . Sexual activity has been shown to shorten the lifespan of both male and female flies [14,64–66]. Thus, flies that are allowed to mate freely throughout life maintained on high-nutrient food will be shorter lived than those on low-nutrient food, due to differences in sexual activity, as well as differences caused by direct nutritional effects of DR. For this reason, it is essential for any study investigating the effects of DR on fly lifespan to control mating status; for instance, by using single-sex cohorts. At this stage it is unknown whether male–male or female–female interactions such as fighting  also affect lifespan in a nutrient-level-dependent manner. While the effect of adult density on lifespan is already known , the difference in longevity between individually and group-housed flies at different food levels remains untested.
Many studies that have used Drosophila to investigate the mechanisms of DR have done so using mixed-sex groups [20,26,50,69–77] (Table S1). From this body of work, it has been concluded that p53, SIR2, and resveratrol function in the same pathway, which is essential for DR's lifespan-extending effects [73–75]. However, the use of mixed-sex groups without controlling for mating status in these experiments means it is impossible to discern the interaction between genotype or drug treatment and diet on lifespan, because sexual activity will act as a confounding variable. Thus, it is important for future work on flies, as well as comparative mechanistic studies of DR that flies are housed in single-sex cohorts, or even singly, for experiments.
Aging, Diseases, and Death
The long history of DR studies in rodents has provided us with a great deal of data that show that, with few exceptions, DR extends the lifespan of a variety of lab-maintained strains of rats and mice . Many of these are inbred strains that are theoretically genetically homogenous and homozygous at almost all loci. Inevitably, different inbred lines become prone to different aging-related disorders , and yet they generally respond to DR with extended lifespan and a delay in a host of aging related diseases [7,39,40,79–81]. What is more, rodent models of specific diseases that are associated with aging, such as Alzheimer disease, appear to respond favourably to DR, with a delay in disease [82,83]. These observations and DR's ability to slow a wide array of aging-related disorders, as well as the evolutionary conservation of its lifespan-extending effect, can be interpreted to indicate that DR affects the root of aging itself and is not just reducing a specific disease by diet interaction . If a similarly deep-rooted mechanism is at work in flies, a direct comparison of a variety of inbred laboratory lines of Drosophila should reveal that most respond to DR. This should also be true of flies affected by specific aging-related disorders, such as the fly model of Alzheimer disease . As a by-product of this work, any mutants or inbred lines that do not respond to DR will be important tools in future work to uncover the mechanisms of lifespan extension.
Despite many years of work, the mechanisms that underlie the effect of DR on lifespan remain unknown. Although historically much of the work has been performed with rodents, large-scale lifespan experiments under many conditions and genetic analysis are better suited to shorter-lived, and more easily housed, model organisms such as the invertebrates. However, if work in invertebrates is to be of any relevance to the study of aging in higher organisms, it is important to establish techniques that eliminate the confounding effects of nonaging-related causes of death, such as food toxicity and altered sexual activity. Only then can the mechanistic relationship between diet and death be established, providing modes of action to be tested in the longer-lived models. To test whether the mechanisms of DR are likely to be conserved from flies to mice, it will be interesting to see if Drosophila of different inbred lines or disease models respond to DR in a manner similar to their rodent counterparts. These studies are likely to be further refined by dietary interventions that focus on altering specific nutrients and on discovering mutations that block or alter the response to DR. What is exciting about studying DR in Drosophila is that each of the tools to perform these investigations are currently available or within close reach, meaning that characterisation of DR in Drosophila is likely to continue to be a fertile ground for research.
Table S1. Summary of Various DR Experiments Performed with Flies
(96 KB DOC)
Video S1. Female Drosophila Feeding on DR and Control Media
(9.2 MB WMV)
- 1. Alpatov WW (1930) Experimental studies on the duration of life. XIII. The influence of different feeding during the larval and imaginal stages on the duration of life of the imago of Drosophila melanogaster. American Naturalist 64: 37–55.WW Alpatov1930Experimental studies on the duration of life. XIII. The influence of different feeding during the larval and imaginal stages on the duration of life of the imago of Drosophila melanogaster.American Naturalist643755
- 2. Loeb J, Northrop JH (1917) On the influence of food and temperature upon the duration of life. J Biol Chem 32: 103–121.J. LoebJH Northrop1917On the influence of food and temperature upon the duration of life.J Biol Chem32103121
- 3. Pearl R (1921) The biology of death–VI. Experimental studies on the duration of life. The Scientific Monthly 13: 144–164.R. Pearl1921The biology of death–VI. Experimental studies on the duration of life.The Scientific Monthly13144164
- 4. Pearl R (1928) The rate of living. London: University of London Press. R. Pearl1928The rate of livingLondonUniversity of London Press185
- 5. Kopec S (1928) On the influence of intermittent starvation on the longevity of the imaginal stage of Drosophila melanogaster. British J Exp Biol 5: 204–211.S. Kopec1928On the influence of intermittent starvation on the longevity of the imaginal stage of Drosophila melanogaster.British J Exp Biol5204211
- 6. McCay CM, Crowell MF, Maynard LA (1935) The effect of retarded growth upon the length of lifespan and upon the ultimate body size. J Nutr 10: 63–79.CM McCayMF CrowellLA Maynard1935The effect of retarded growth upon the length of lifespan and upon the ultimate body size.J Nutr106379
- 7. Weindruch R, Walford RL (1988) The retardation of aging and disease by dietary restriction. Springfield (Illinois): Thomas. R. WeindruchRL Walford1988The retardation of aging and disease by dietary restrictionSpringfield (Illinois)Thomas 436
- 8. Partridge L, Piper MD, Mair W (2005) Dietary restriction in Drosophila. Mech Ageing Dev 126: 938–950.L. PartridgeMD PiperW. Mair2005Dietary restriction in Drosophila.Mech Ageing Dev126938950
- 9. Le Bourg E, Medioni J (1991) Food restriction and longevity in Drosophila melanogaster. Age Nutr 2: 90–94.E. Le BourgJ. Medioni1991Food restriction and longevity in Drosophila melanogaster.Age Nutr29094
- 10. Le Bourg E, Minois N (1996) Failure to confirm increased longevity in Drosophila melanogaster submitted to a food restriction procedure. J Gerontol A Biol Sci Med Sci 51: B280–B283.E. Le BourgN. Minois1996Failure to confirm increased longevity in Drosophila melanogaster submitted to a food restriction procedure.J Gerontol A Biol Sci Med Sci51B280B283
- 11. Carey JR, Liedo P, Harshman L, Zhang Y, Muller HG, et al. (2002) Life history response of Mediterranean fruit flies to dietary restriction. Aging Cell 1: 140–148.JR CareyP. LiedoL. HarshmanY. ZhangHG Muller2002Life history response of Mediterranean fruit flies to dietary restriction.Aging Cell1140148
- 12. Cooper TM, Mockett RJ, Sohal BH, Sohal RS, Orr WC (2004) Effect of caloric restriction on life span of the housefly, Musca domestica. FASEB J 18: 1591–1593.TM CooperRJ MockettBH SohalRS SohalWC Orr2004Effect of caloric restriction on life span of the housefly, Musca domestica.FASEB J1815911593
- 13. Le Bourg E, Minois N (2005) Does dietary restriction really increase longevity in Drosophila melanogaster? Ageing Res Rev 4: 409–421.E. Le BourgN. Minois2005Does dietary restriction really increase longevity in Drosophila melanogaster?Ageing Res Rev4409421
- 14. Partridge L, Green A, Fowler K (1987) Effects of egg-production and of exposure to males on female survival in Drosophila melanogaster. J Insect Physiol 33: 745–749.L. PartridgeA. GreenK. Fowler1987Effects of egg-production and of exposure to males on female survival in Drosophila melanogaster.J Insect Physiol33745749
- 15. Mair W, Piper MDW, Partridge L (2005) Calories do not explain extension of life span by dietary restriction in Drosophila. PLoS Biol 7: e223.. W. MairMDW PiperL. Partridge2005Calories do not explain extension of life span by dietary restriction in Drosophila.PLoS Biol7e223.
- 16. Ashburner M (1989) Appendix N: Food media. Drosophila. A laboratory manual. Cold Spring Harbor (New York): Cold Spring Harbor Laboratory Press. pp. 399–402.M. Ashburner1989Appendix N: Food media.In:. Drosophila. A laboratory manualCold Spring Harbor (New York)Cold Spring Harbor Laboratory Press399402
- 17. Chapman T, Partridge L (1996) Female fitness in Drosophila melanogaster: An interaction between the effect of nutrition and of encounter rate with males. Proc R Soc Lond B Biol Sci 263: 755–759.T. ChapmanL. Partridge1996Female fitness in Drosophila melanogaster: An interaction between the effect of nutrition and of encounter rate with males.Proc R Soc Lond B Biol Sci263755759
- 18. Partridge L, Gems D, Withers DJ (2005) Sex and death: What is the connection? Cell 120: 461–472.L. PartridgeD. GemsDJ Withers2005Sex and death: What is the connection?Cell120461472
- 19. Carvalho GB, Kapahi P, Benzer S (2005) Compensatory ingestion upon dietary restriction in Drosophila melanogaster. Nat Methods 2: 813–815.GB CarvalhoP. KapahiS. Benzer2005Compensatory ingestion upon dietary restriction in Drosophila melanogaster.Nat Methods2813815
- 20. Min KJ, Tatar M (2006) Drosophila diet restriction in practice: Do flies consume fewer nutrients? Mech Ageing Dev 127: 93–96.KJ MinM. Tatar2006Drosophila diet restriction in practice: Do flies consume fewer nutrients?Mech Ageing Dev1279396
- 21. Carvalho GB, Kapahi P, Anderson DJ, Benzer S (2006) Allocrine modulation of feeding behavior by the sex peptide of Drosophila. Curr Biol 16: 692–696.GB CarvalhoP. KapahiDJ AndersonS. Benzer2006Allocrine modulation of feeding behavior by the sex peptide of Drosophila.Curr Biol16692696
- 22. Simpson SJ, Barton Browne L, van Gerwen ACM (1989) The patterning of compensatory sugar feeding in the Australian sheep blowfly. Physiol Entomol 14: 19–105.SJ SimpsonL. Barton BrowneACM van Gerwen1989The patterning of compensatory sugar feeding in the Australian sheep blowfly.Physiol Entomol1419105
- 23. Sondergaard L, Mauchline D, Egetoft P, White N, Wulff P, et al. (1995) Nutritional response in a Drosophila yolk protein gene promoter. Mol Gen Genet 248: 25–32.L. SondergaardD. MauchlineP. EgetoftN. WhiteP. Wulff1995Nutritional response in a Drosophila yolk protein gene promoter.Mol Gen Genet2482532
- 24. Terashima J, Bownes M (2004) Translating available food into the number of eggs laid by Drosophila melanogaster. Genetics 167: 1711–1719.J. TerashimaM. Bownes2004Translating available food into the number of eggs laid by Drosophila melanogaster.Genetics16717111719
- 25. Terashima J, Takaki K, Sakurai S, Bownes M (2005) Nutritional status affects 20-hydroxyecdysone concentration and progression of oogenesis in Drosophila melanogaster. J Endocrinol 187: 69–79.J. TerashimaK. TakakiS. SakuraiM. Bownes2005Nutritional status affects 20-hydroxyecdysone concentration and progression of oogenesis in Drosophila melanogaster.J Endocrinol1876979
- 26. Chippindale AK, Leroi AM, Kim SB, Rose MR (1993) Phenotypic plasticity and selection in Drosophila life-history evolution. I. Nutrition and the cost of reproduction. J Evol Biol 6: 171–193.AK ChippindaleAM LeroiSB KimMR Rose1993Phenotypic plasticity and selection in Drosophila life-history evolution. I. Nutrition and the cost of reproduction.J Evol Biol6171193
- 27. Bass TM, Piper MD, Grandison RC, Wong R, Martinez P, et al. (2007) Optimization of dietary resitrction protocols for Drosophila to avoid food toxicity. J Gerontol. TM BassMD PiperRC GrandisonR. WongP. Martinez2007Optimization of dietary resitrction protocols for Drosophila to avoid food toxicity.J GerontolIn press. In press.
- 28. Gems D, Riddle DL (2000) Genetic, behavioral and environmental determinants of male longevity in Caenorhabditis elegans. Genetics 154: 1597–1610.D. GemsDL Riddle2000Genetic, behavioral and environmental determinants of male longevity in Caenorhabditis elegans.Genetics15415971610
- 29. Garigan D, Hsu AL, Fraser AG, Kamath RS, Ahringer J, et al. (2002) Genetic analysis of tissue aging in Caenorhabditis elegans: A role for heat-shock factor and bacterial proliferation. Genetics 161: 1101–1112.D. GariganAL HsuAG FraserRS KamathJ. Ahringer2002Genetic analysis of tissue aging in Caenorhabditis elegans: A role for heat-shock factor and bacterial proliferation.Genetics16111011112
- 30. Kaeberlein TL, Smith ED, Tsuchiya M, Welton KL, Thomas JH, et al. (2006) Lifespan extension in Caenorhabditis elegans by complete removal of food. Aging Cell 5: 487–494.TL KaeberleinED SmithM. TsuchiyaKL WeltonJH Thomas2006Lifespan extension in Caenorhabditis elegans by complete removal of food.Aging Cell5487494
- 31. Lee GD, Wilson MA, Zhu M, Wolkow CA, de Cabo R, et al. (2006) Dietary deprivation extends lifespan in Caenorhabditis elegans. Aging Cell 5: 515–524.GD LeeMA WilsonM. ZhuCA WolkowR. de Cabo2006Dietary deprivation extends lifespan in Caenorhabditis elegans.Aging Cell5515524
- 32. Garsin DA, Sifri CD, Mylonakis E, Qin X, Singh KV, et al. (2001) A simple model host for identifying Gram-positive virulence factors. Proc Natl Acad Sci U S A 98: 10892–10897.DA GarsinCD SifriE. MylonakisX. QinKV Singh2001A simple model host for identifying Gram-positive virulence factors.Proc Natl Acad Sci U S A981089210897
- 33. Garsin DA, Villanueva JM, Begun J, Kim DH, Sifri CD, et al. (2003) Long-lived C. elegans daf-2 mutants are resistant to bacterial pathogens. Science 300: 1921.DA GarsinJM VillanuevaJ. BegunDH KimCD Sifri2003Long-lived C. elegans daf-2 mutants are resistant to bacterial pathogens.Science3001921
- 34. Martin I, Grotewiel MS (2006) Distinct genetic influences on locomotor senescence in Drosophila revealed by a series of metrical analyses. Exp Gerontol 41: 877–881.I. MartinMS Grotewiel2006Distinct genetic influences on locomotor senescence in Drosophila revealed by a series of metrical analyses.Exp Gerontol41877881
- 35. Driver CJ, Cosopodiotis G (1979) The effect of dietary fat on longevity of Drosophila melanogaster. Exp Gerontol 14: 95–100.CJ DriverG. Cosopodiotis1979The effect of dietary fat on longevity of Drosophila melanogaster.Exp Gerontol1495100
- 36. Driver CJ, Lamb MJ (1980) Metabolic changes in ageing Drosophila melanogaster. Exp Gerontol 15: 167–175.CJ DriverMJ Lamb1980Metabolic changes in ageing Drosophila melanogaster.Exp Gerontol15167175
- 37. Driver CJ, Wallis R, Cosopodiotis G, Ettershank G (1986) Is a fat metabolite the major diet dependent accelerator of aging? Exp Gerontol 21: 497–507.CJ DriverR. WallisG. CosopodiotisG. Ettershank1986Is a fat metabolite the major diet dependent accelerator of aging?Exp Gerontol21497507
- 38. Masoro EJ (2005) Overview of caloric restriction and ageing. Mech Ageing Dev 126: 913–922.EJ Masoro2005Overview of caloric restriction and ageing.Mech Ageing Dev126913922
- 39. Iwasaki K, Gleiser CA, Masoro EJ, McMahan CA, Seo EJ, et al. (1988) The influence of dietary protein source on longevity and age-related disease processes of Fischer rats. J Gerontol 43: B5–B12.K. IwasakiCA GleiserEJ MasoroCA McMahanEJ Seo1988The influence of dietary protein source on longevity and age-related disease processes of Fischer rats.J Gerontol43B5B12
- 40. Masoro EJ, Iwasaki K, Gleiser CA, McMahan CA, Seo EJ, et al. (1989) Dietary modulation of the progression of nephropathy in aging rats: An evaluation of the importance of protein. Am J Clin Nutr 49: 1217–1227.EJ MasoroK. IwasakiCA GleiserCA McMahanEJ Seo1989Dietary modulation of the progression of nephropathy in aging rats: An evaluation of the importance of protein.Am J Clin Nutr4912171227
- 41. Goodrick CL, Ingram DK, Reynolds MA, Freeman JR, Cider N (1990) Effects of intermittent feeding upon body weight and lifespan in inbred mice: Interaction of genotype and age. Mech Ageing Dev 55: 69–87.CL GoodrickDK IngramMA ReynoldsJR FreemanN. Cider1990Effects of intermittent feeding upon body weight and lifespan in inbred mice: Interaction of genotype and age.Mech Ageing Dev556987
- 42. Orentreich N, Matias JR, DeFelice A, Zimmerman JA (1993) Low methionine ingestion by rats extends life span. J Nutr 123: 269–274.N. OrentreichJR MatiasA. DeFeliceJA Zimmerman1993Low methionine ingestion by rats extends life span.J Nutr123269274
- 43. Anson RM, Guo Z, de Cabo R, Iyun T, Rios M, et al. (2003) Intermittent fasting dissociates beneficial effects of dietary restriction on glucose metabolism and neuronal resistance to injury from calorie intake. Proc Natl Acad Sci U S A 100: 6216–6220.RM AnsonZ. GuoR. de CaboT. IyunM. Rios2003Intermittent fasting dissociates beneficial effects of dietary restriction on glucose metabolism and neuronal resistance to injury from calorie intake.Proc Natl Acad Sci U S A10062166220
- 44. Zimmerman JA, Malloy V, Krajcik R, Orentreich N (2003) Nutritional control of aging. Exp Gerontol 38: 47–52.JA ZimmermanV. MalloyR. KrajcikN. Orentreich2003Nutritional control of aging.Exp Gerontol384752
- 45. Miller RA, Buehner G, Chang Y, Harper JM, Sigler R, et al. (2005) Methionine-deficient diet extends mouse lifespan, slows immune and lens aging, alters glucose, T4, IGF-I and insulin levels, and increases hepatocyte MIF levels and stress resistance. Aging Cell 4: 119–125.RA MillerG. BuehnerY. ChangJM HarperR. Sigler2005Methionine-deficient diet extends mouse lifespan, slows immune and lens aging, alters glucose, T4, IGF-I and insulin levels, and increases hepatocyte MIF levels and stress resistance.Aging Cell4119125
- 46. Piper MD, Mair W, Partridge L (2005) Counting the calories: The role of specific nutrients in extension of life span by food restriction. J Gerontol A Biol Sci Med Sci 60: 549–555.MD PiperW. MairL. Partridge2005Counting the calories: The role of specific nutrients in extension of life span by food restriction.J Gerontol A Biol Sci Med Sci60549555
- 47. Alcedo J, Kenyon C (2004) Regulation of C. elegans longevity by specific gustatory and olfactory neurons. Neuron 41: 45–55.J. AlcedoC. Kenyon2004Regulation of C. elegans longevity by specific gustatory and olfactory neurons.Neuron414555
- 48. Libert S, Zwiener J, Chu X, Vanvoorhies W, Roman G, Pletcher SD (2007) Regulation of Drosophila lifespan by olfaction and food-derived odors. Science 315: 1133–1137.S. LibertJ. ZwienerX. ChuW. VanvoorhiesG. RomanSD Pletcher2007Regulation of Drosophila lifespan by olfaction and food-derived odors.Science31511331137
- 49. De Marte ML, Enesco HE (1986) Influence of low tryptophan diet on survival and organ growth in mice. Mech Ageing Dev 36: 161–171.ML De MarteHE Enesco1986Influence of low tryptophan diet on survival and organ growth in mice.Mech Ageing Dev36161171
- 50. Min KJ, Tatar M (2006) Restriction of amino acids extends lifespan in Drosophila melanogaster. Mech Ageing Dev 127: 643–646.KJ MinM. Tatar2006Restriction of amino acids extends lifespan in Drosophila melanogaster.Mech Ageing Dev127643646
- 51. Martinez-Vicente M, Sovak G, Cuervo AM (2005) Protein degradation and aging. Exp Gerontol 40: 622–633.M. Martinez-VicenteG. SovakAM Cuervo2005Protein degradation and aging.Exp Gerontol40622633
- 52. Terman A (2006) Catabolic insufficiency and aging. Ann N Y Acad Sci 1067: 27–36.A. Terman2006Catabolic insufficiency and aging.Ann N Y Acad Sci10672736
- 53. Massey AC, Zhang C, Cuervo AM (2006) Chaperone-mediated autophagy in aging and disease. Curr Top Dev Biol 73: 205–235.AC MasseyC. ZhangAM Cuervo2006Chaperone-mediated autophagy in aging and disease.Curr Top Dev Biol73205235
- 54. Jacinto E, Hall MN (2003) Tor signalling in bugs, brain and brawn. Nat Rev Mol Cell Biol 4: 117–126.E. JacintoMN Hall2003Tor signalling in bugs, brain and brawn.Nat Rev Mol Cell Biol4117126
- 55. Scott RC, Schuldiner O, Neufeld TP (2004) Role and regulation of starvation-induced autophagy in the Drosophila fat body. Dev Cell 7: 167–178.RC ScottO. SchuldinerTP Neufeld2004Role and regulation of starvation-induced autophagy in the Drosophila fat body.Dev Cell7167178
- 56. Rusten TE, Lindmo K, Juhasz G, Sass M, Seglen PO, et al. (2004) Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway. Dev Cell 7: 179–192.TE RustenK. LindmoG. JuhaszM. SassPO Seglen2004Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway.Dev Cell7179192
- 57. Droge W (2004) Autophagy and aging–importance of amino acid levels. Mech Ageing Dev 125: 161–168.W. Droge2004Autophagy and aging–importance of amino acid levels.Mech Ageing Dev125161168
- 58. Melendez A, Talloczy Z, Seaman M, Eskelinen EL, Hall DH, et al. (2003) Autophagy genes are essential for dauer development and life-span extension in C. elegans. Science 301: 1387–1391.A. MelendezZ. TalloczyM. SeamanEL EskelinenDH Hall2003Autophagy genes are essential for dauer development and life-span extension in C. elegans.Science30113871391
- 59. Hamilton WD (1966) The moulding of senescence by natural selection. J Theor Biol 12: 12–45.WD Hamilton1966The moulding of senescence by natural selection.J Theor Biol121245
- 60. Kirkwood TB (1977) Evolution of ageing. Nature 270: 301–304.TB Kirkwood1977Evolution of ageing.Nature270301304
- 61. Holliday R (1989) Food, reproduction and longevity: Is the extended lifespan of calorie-restricted animals an evolutionary adaptation? Bioessays 10: 125–127.R. Holliday1989Food, reproduction and longevity: Is the extended lifespan of calorie-restricted animals an evolutionary adaptation?Bioessays10125127
- 62. Harrison DE, Archer JR (1989) Natural selection for extended longevity from food restriction. Growth Dev Aging 53: 3–6.DE HarrisonJR Archer1989Natural selection for extended longevity from food restriction.Growth Dev Aging5336
- 63. Trevitt S, Fowler K, Partridge L (1988) An effect of egg-deposition on the subsequent fertility and remating frequency of female Drosophila melanogaster. J Insect Physiol 34: 821–828.S. TrevittK. FowlerL. Partridge1988An effect of egg-deposition on the subsequent fertility and remating frequency of female Drosophila melanogaster.J Insect Physiol34821828
- 64. Partridge L, Farquhar M (1981) Sexual activity reduces lifespan of male fruitflies. Nature 294: 580–582.L. PartridgeM. Farquhar1981Sexual activity reduces lifespan of male fruitflies.Nature294580582
- 65. Fowler K, Partridge L (1989) A cost of mating in female fruitflies. Nature 338: 760–761.K. FowlerL. Partridge1989A cost of mating in female fruitflies.Nature338760761
- 66. Wigby S, Chapman T (2005) Sex peptide causes mating costs in female Drosophila melanogaster. Curr Biol 15: 316–321.S. WigbyT. Chapman2005Sex peptide causes mating costs in female Drosophila melanogaster.Curr Biol15316321
- 67. Chen S, Lee AY, Bowens NM, Huber R, Kravitz EA (2002) Fighting fruit flies: A model system for the study of aggression. Proc Natl Acad Sci U S A 99: 5664–5668.S. ChenAY LeeNM BowensR. HuberEA Kravitz2002Fighting fruit flies: A model system for the study of aggression.Proc Natl Acad Sci U S A9956645668
- 68. Pearl R (1928) Experiments on longevity. Experiments on longevity 3: 391–407.R. Pearl1928Experiments on longevity.Experiments on longevity3391407
- 69. David J, van Herrewege J, Fouillet P (1971) Quantitative under-feeding of Drosophila: Effects on adult longevity and fecundity. Exp Gerontol 6: 249–257.J. DavidJ. van HerrewegeP. Fouillet1971Quantitative under-feeding of Drosophila: Effects on adult longevity and fecundity.Exp Gerontol6249257
- 70. Van Herrewege J (1974) Nutritional requirements of adult Drosophila melanogaster: The influence of the casein concentration on the duration of life. Exp Gerontol 9: 191–198.J. Van Herrewege1974Nutritional requirements of adult Drosophila melanogaster: The influence of the casein concentration on the duration of life.Exp Gerontol9191198
- 71. Simmons FH, Bradley TJ (1997) An analysis of resource allocation in response to dietary yeast in Drosophila melanogaster. J Insect Physiol 43: 779–788.FH SimmonsTJ Bradley1997An analysis of resource allocation in response to dietary yeast in Drosophila melanogaster.J Insect Physiol43779788
- 72. Good TP, Tatar M (2001) Age-specific mortality and reproduction respond to adult dietary restriction in Drosophila melanogaster. J Insect Physiol 47: 1467–1473.TP GoodM. Tatar2001Age-specific mortality and reproduction respond to adult dietary restriction in Drosophila melanogaster.J Insect Physiol4714671473
- 73. Rogina B, Helfand SL (2004) Sir2 mediates longevity in the fly through a pathway related to calorie restriction. Proc Natl Acad Sci U S A 101: 12980–12985.B. RoginaSL Helfand2004Sir2 mediates longevity in the fly through a pathway related to calorie restriction.Proc Natl Acad Sci U S A1011298012985
- 74. Wood JG, Rogina B, Lavu S, Howitz K, Helfand SL, et al. (2004) Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature 430: 686–689.JG WoodB. RoginaS. LavuK. HowitzSL Helfand2004Sirtuin activators mimic caloric restriction and delay ageing in metazoans.Nature430686689
- 75. Bauer JH, Poon PC, Glatt-Deeley H, Abrams JM, Helfand SL (2005) Neuronal expression of p53 dominant-negative proteins in adult Drosophila melanogaster extends life span. Curr Biol 15: 2063–2068.JH BauerPC PoonH. Glatt-DeeleyJM AbramsSL Helfand2005Neuronal expression of p53 dominant-negative proteins in adult Drosophila melanogaster extends life span.Curr Biol1520632068
- 76. Bross TG, Rogina B, Helfand SL (2005) Behavioral, physical, and demographic changes in Drosophila populations through dietary restriction. Aging Cell 4: 309–317.TG BrossB. RoginaSL Helfand2005Behavioral, physical, and demographic changes in Drosophila populations through dietary restriction.Aging Cell4309317
- 77. Zheng J, Mutcherson R, Helfand SL (2005) Calorie restriction delays lipid oxidative damage in Drosophila melanogaster. Aging Cell 4: 209–216.J. ZhengR. MutchersonSL Helfand2005Calorie restriction delays lipid oxidative damage in Drosophila melanogaster.Aging Cell4209216
- 78. Taft RA, Davisson M, Wiles MV (2006) Know thy mouse. Trends Genet 22: 649–653.RA TaftM. DavissonMV Wiles2006Know thy mouse.Trends Genet22649653
- 79. Iwasaki K, Gleiser CA, Masoro EJ, McMahan CA, Seo EJ, et al. (1988) Influence of the restriction of individual dietary components on longevity and age-related disease of Fischer rats: The fat component and the mineral component. J Gerontol 43: B13–B21.K. IwasakiCA GleiserEJ MasoroCA McMahanEJ Seo1988Influence of the restriction of individual dietary components on longevity and age-related disease of Fischer rats: The fat component and the mineral component.J Gerontol43B13B21
- 80. Masoro EJ, Shimokawa I, Yu BP (1991) Retardation of the aging processes in rats by food restriction. Ann N Y Acad Sci 621: 337–352.EJ MasoroI. ShimokawaBP Yu1991Retardation of the aging processes in rats by food restriction.Ann N Y Acad Sci621337352
- 81. Weindruch R, Walford RL (1982) Dietary restriction in mice beginning at 1 year of age: Effect on life-span and spontaneous cancer incidence. Science 215: 1415–1418.R. WeindruchRL Walford1982Dietary restriction in mice beginning at 1 year of age: Effect on life-span and spontaneous cancer incidence.Science21514151418
- 82. Mattson MP (2003) Will caloric restriction and folate protect against AD and PD? Neurology 60: 690–695.MP Mattson2003Will caloric restriction and folate protect against AD and PD?Neurology60690695
- 83. Patel NV, Gordon MN, Connor KE, Good RA, Engelman RW, et al. (2005) Caloric restriction attenuates Abeta-deposition in Alzheimer transgenic models. Neurobiol Aging 26: 995–1000.NV PatelMN GordonKE ConnorRA GoodRW Engelman2005Caloric restriction attenuates Abeta-deposition in Alzheimer transgenic models.Neurobiol Aging269951000
- 84. Crowther DC, Page R, Chandraratna D, Lomas DA (2006) A Drosophila model of Alzheimer's disease. Methods Enzymol 412: 234–255.DC CrowtherR. PageD. ChandraratnaDA Lomas2006A Drosophila model of Alzheimer's disease.Methods Enzymol412234255