The human enteropathogen, Yersinia enterocolitica, is a significant link in the range of Yersinia pathologies extending from mild gastroenteritis to bubonic plague. Comparison at the genomic level is a key step in our understanding of the genetic basis for this pathogenicity spectrum. Here we report the genome of Y. enterocolitica strain 8081 (serotype 0:8; biotype 1B) and extensive microarray data relating to the genetic diversity of the Y. enterocolitica species. Our analysis reveals that the genome of Y. enterocolitica strain 8081 is a patchwork of horizontally acquired genetic loci, including a plasticity zone of 199 kb containing an extraordinarily high density of virulence genes. Microarray analysis has provided insights into species-specific Y. enterocolitica gene functions and the intraspecies differences between the high, low, and nonpathogenic Y. enterocolitica biotypes. Through comparative genome sequence analysis we provide new information on the evolution of the Yersinia. We identify numerous loci that represent ancestral clusters of genes potentially important in enteric survival and pathogenesis, which have been lost or are in the process of being lost, in the other sequenced Yersinia lineages. Our analysis also highlights large metabolic operons in Y. enterocolitica that are absent in the related enteropathogen, Yersinia pseudotuberculosis, indicating major differences in niche and nutrients used within the mammalian gut. These include clusters directing, the production of hydrogenases, tetrathionate respiration, cobalamin synthesis, and propanediol utilisation. Along with ancestral gene clusters, the genome of Y. enterocolitica has revealed species-specific and enteropathogen-specific loci. This has provided important insights into the pathology of this bacterium and, more broadly, into the evolution of the genus. Moreover, wider investigations looking at the patterns of gene loss and gain in the Yersinia have highlighted common themes in the genome evolution of other human enteropathogens.
The goal of this study was to catalogue all the genes encoded within the Y. enterocolitica genome to help us better understand how this bacterium and related bacteria cause different diseases. There are currently genome sequences (complete gene catalogues) available for two other members of this bacterial lineage, which cause dramatically different diseases: Y. pseudotuberculosis, like Y. enterocolitica, is a gut pathogen (enteropathogen) causing gastroenteritis in humans and animals. Yersinia pestis mostly resides within blood (circulating or in fleas following blood meals) and lymph tissue. It causes bubonic plague in humans and animals, and is historically known as “The Black Death.” A three-way comparison of these genomes revealed a patchwork of genes we have defined as being species- or disease-specific and genes that are common to all three Yersinia species. This has provided us with important information on shared gene functions that define the two enteropathogenic yersinias and those that differentiate them. This will help us to connect what we know about the Y. enterocolitica lifestyle within the gut to the disease it causes and its genetic makeup. We have also provided further evidence of gene-loss by Y. pestis as it has evolved from Y. pseudotuberculosis into a more acute systemic pathogen. Similar patterns of gene loss are seen in other important pathogens such as Salmonella enterica serovar Typhi.
Citation: Thomson NR, Howard S, Wren BW, Holden MTG, Crossman L, Challis GL, et al. (2006) The Complete Genome Sequence and Comparative Genome Analysis of the High Pathogenicity Yersinia enterocolitica Strain 8081. PLoS Genet2(12): e206. https://doi.org/10.1371/journal.pgen.0020206
Editor: Paul M. Richardson, Department of Energy Joint Genome Institute, United States of America
Received: August 14, 2006; Accepted: October 20, 2006; Published: December 15, 2006
Copyright: © 2006 Thomson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by The Wellcome Trust.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: bp, base pair; CDS, coding sequence; CRISPR, clustered regularly interspaced short palindromic repeats; GSP, general secretion pathway; kb, kilobase; MTA, methylthioadenosine; OPG, osmoregulated periplasmic glucan; pYV, Yersinia virulence plasmid; PZ, plasticity zone; TTSS, type three secretion system; YGI-1, Yersinia genomic island 1; YGI-2, Yersinia genomic island 2; YGI-3, Yersinia genomic island 3; YGI-4, Yersinia genomic island 4
Y. enterocolitica is a globally distributed gastrointestinal pathogen that represents a key link in our understanding of how the three human pathogenic Yersinia species, Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis, have evolved to produce diverse clinical manifestations. Like Y. enterocolitica, Y. pseudotuberculosis is an enteropathogen that is widely found in the environment, but it causes more severe clinical manifestations than Y. enterocolitica . Y. pestis is primarily a rodent pathogen that is transmitted by the bite of an infected flea, and causes the often fatal systemic infection, bubonic plague . Multilocus sequence analysis and DNA–DNA hybridization studies suggest that Y. enterocolitica and Y. pseudotuberculosis diverged within the last 200 million years and that Y. pestis is a clone of Y. pseudotuberculosis that has emerged within the last 1,500–20,000 years [3–5].
Since the pathogenic yersiniae diverged, Y. enterocolitica has evolved into an apparently heterogeneous collection of organisms encompassing six biotypes differentiated by biochemical tests (1A, 1B, 2, 3, 4, and 5) . These in vitro biotypes group into three distinct grades of pathogen: a mostly nonpathogenic group (biogroup 1A); weakly pathogenic groups that are unable to kill mice (biogroups 2–5); and a highly pathogenic, mouse-lethal group (biogroup 1B) [6–9]. These biogroups have geographically distinct distributions, with biotype 1B being most frequently isolated in North America (termed the “New-World” strains), whereas biogroups 2–5 predominate in Europe and Japan (termed the “Old-World” strains) [10,11].
It is clear that DNA acquisition by lateral gene transfer has been fundamental in the emergence of the pathogenic yersiniae, all of which possess a 70-kilobase (kb) virulence plasmid (pYV) [12,13] and carry additional genetic factors located on the chromosome that are important for virulence [14–17]. However, current knowledge of the genetic repertoire that differentiates these strains is incomplete. Representatives of the two other human pathogenic Yersinia species, Y. pseudotuberculosis strain IP32953 (referred to as Y. pseudotuberculosis), and Y. pestis (strains CO92 [biovar Orientalis], KIM10+ [biovar Mediaevalis], and 91001 [biovar Microtis]; unless stated otherwise, all further references to Y. pestis relate to strain CO92), have been sequenced [18–21]. To define key steps in the evolution of the pathogenic yersiniae, we sought to define the genetic factors that were conserved in all of the pathogenic species from those that distinguish Y. enterocolitica. In addition, since Y. enterocolitica is a heterogeneous species we undertook microarray analysis aimed at relating the insights gained from the sequence data of strain 8081 biotype 1B to the other Y. enterocolitica biotypes.
The genome of Y. enterocolitica is very similar in size, number of predicted genes, and nucleotide composition to those of Y. pestis and Y. pseudotuberculosis (for a summary see Figure 1 and Table 1). The most notable differences lie in the numbers of insertion-sequence elements and pseudogenes. Although the total number of insertion-sequence elements carried by Y. enterocolitica is lower than the other yersiniae, their diversity is greater, due to a recent expansion of a few elements in Y. pestis (see Table S1).
The outer scale shows the size in bps. From the outside in, circles 1 and 2 show the position of CDSs transcribed in a clockwise and anticlockwise direction, respectively (for colour codes see below). Circles 3–5 (all CDSs coloured green) mark the position of Y. enterocolitica strain 8081 genes that have orthologues (by reciprocal FASTA analysis) in Y. pestis strains CO92, 91001, and KIM10+ and in (circle 6) Y. pseudotuberculosis strain IP32953 (CDSs coloured orange), respectively. Circles 7–10 show the Y. enterocolitica strain 8081 CDSs present (as detected by microarray) in all of the Y. enterocolitica isolates tested from biotype 1A (eight strains, red), biotype 2 (two strains, pink), biotype 3 (eight strains, blue), and biotype 4 (eight strains, yellow). Circle 11 shows CDSs unique to Y. enterocolitica strain 8081 (brown) compared with Y. pestis strain CO92 and Y. pseudotuberculosis strain IP32953 as determined by reciprocal FASTA analysis. Circle 12 shows CDSs unique to Y. enterocolitica strain 8081 (black) biotype 1B compared to all isolates of Y. enterocolitica biotypes 1A, 2, 3, and 4 as determined by microarray analysis. Circle 13 shows a plot of G + C content (in a 10-kb window) and circle 14 shows a plot of GC skew ([G − C]/[G + C] in a 10-kb window). Genes in circles 1 and 2 are colour-coded according to the function of their gene products: dark green, membrane or surface structures; yellow, central or intermediary metabolism; cyan, degradation of macromolecules; red, information transfer/cell division; cerise, degradation of small molecules; pale blue, regulators; salmon pink, pathogenicity or adaptation; black, energy metabolism; orange, conserved hypothetical; pale green, unknown; and brown, pseudogenes. The position of prophage elements (pink) and other important regions of difference (mentioned in the text) are marked (red). See Table 2 for a description.
LPS, lipopolysaccharide biosynthetic genes.
Properties of All the Published Yersinia Genomes
Significant Regions of Difference Identified in Chromosomal Gene Repertoire between the Yersinia Identified by Genome Sequencing and Microarray Analysis
Y. enterocolitica possesses a similar number of pseudogenes (67 coding sequences [CDSs]) to Y. pseudotuberculosis (62 CDSs). This is in contrast to Y. pestis, which is thought to have >140 chromosomal pseudogenes derived from point mutations, insertion sequence element insertions, large-scale rearrangements, and deletions, reflecting a marked change in lifestyle (associated with specific plasmid-acquisition events) [18,19]. This implies that Y. enterocolitica and Y. pseudotuberculosis have been stably maintained in a consistent niche .
Although general features of the Y. enterocolitica genome are similar to those of the other sequenced Yersinia, there is considerable variation in gene repertoire. Reciprocal FastA searches were used to identify orthologous gene sets shared between Y. enterocolitica strain 8081, Y. pestis strain CO92, and Y. pseudotuberculosis strain IP32953 (Figure 2). The yersiniae were found to share 2,747 core CDSs, with a significant number of CDSs being unique to Y. enterocolitica strain 8081 (∼29%), Y. pseudotuberculosis strain IP32953 (∼9%), or Y. pestis strain CO92 (∼11%).
The Venn diagram shows the number of genes unique or shared between two other Yersinia species (see Materials and Methods). The associated pie charts show the breakdown of the functional groups assigned for CDSs in relevant sections of the Venn diagram. Colour code for the pie charts is as follows: hypothetical proteins (1); conserved hypothetical proteins (2); chemotaxis and motility (3); chromosomal replication (4); chaperones (5); protective responses (6); transport and binding proteins (7); adaptations to atypical conditions (8); cell division (9); macromolecule degradation (10); synthesis and modification of macromolecules (11); amino acid biosynthesis (12); biosynthesis of cofactors, prosthetic groups, and carriers (13); central intermediary metabolism (14); small-molecule degradation (15); energy metabolism (16); fatty acid biosynthesis (17); nucleosides and nucleotide biosynthesis and metabolism (18); periplasmic/exported/lipoproteins (19); ribosomal proteins (20); laterally acquired (including prophage CDSs) (21); pathogenicity and virulence (22); general regulation (23); and miscellaneous function (24).
Y. en, Y. enterocolitica strain 8081; Y. pstb, Y. pseudotuberculosis strain IP32953; Y. pestis, Y. pestis strain CO92.
The number of CDSs shared exclusively between Y. enterocolitica and either Y. pseudotuberculosis or Y. pestis was initially surprising (see Figure 2). However, prophage accounted for a significant proportion of these CDSs. These phage-related CDSs are located in distinct gene clusters within different prophage-like elements and so these are unlikely to be true orthologues.
In addition to prophage-related CDSs, CDSs shared between Y. pseudotuberculosis and Y. enterocolitica and absent from Y. pestis fell into a range of other functional categories such as protective responses, adaptation to atypical conditions, and exported proteins (Figure 2). In contrast, CDSs found only in Y. enterocolitica and Y. pestis were either prophage-related or accounted for by differences in annotation. It is highly unlikely that both Y. pseudotuberculosis and Y. enterocolitica independently acquired these functions since the divergence of Y. pseudotuberculosis and Y. pestis; these functions have therefore probably been lost by Y. pestis since diverging from Y. pseudotuberculosis. To investigate this further, we scrutinised the genomic context of the CDSs and identified the corresponding regions in Y. pestis. For some of the Y. pseudotuberculosis- and Y. enterocolitica-specific functions, all indications of their presence in Y. pestis have been lost. However, in several instances it was possible to identify remnants of these regions in Y. pestis. These CDSs may represent ancestral functions important for an enteric lifestyle, but which subsequently became redundant for Y. pestis. Alternatively, given the high virulence potential of Y. pestis, some of these gene changes (gene losses) may be examples of pathoadaptive mutations .
We performed the same analysis for the Y. enterocolitica-specific loci and were able to identify deletion scars (gene remnants) for some of these regions that were apparent in both Y. pestis and Y. pseudotuberculosis; all of these loci are detailed below (summarised in Table 2).
Evidence of Ancestral Yersinia Gene Functions in the Y. enterocolitica Genome
Metabolism and adaptation.
Within the CDSs shared exclusively by Y. enterocolitica and Y. pseudotuberculosis, there are two entire metabolic pathways that have apparently been completely lost by Y. pestis: the methionine-salvage pathway and the osmoregulated periplasmic glucan (OPG) biosynthetic pathway.
The methionine-salvage pathway recycles the sulphur-containing compound, methylthioadenosine (MTA), formed during spermidine and spermine synthesis, and as a byproduct of N-acylhomoserine lactone production. MTA is recycled back to methionine, which can be further metabolised to produce S-adenosylmethionine, an essential reactant in several methylation reactions (see  and references therein).
The methionine-salvage pathways are conserved and appear to be intact in both Y. enterocolitica and Y. pseudotuberculosis. In Y. enterocolitica, the CDSs involved encode MtnK (kinase, YE3228), MntA (isomerase, YE3230), MtnD (dioxygenase, YE3231), MtnC (bifunctional enolase/phosphatase, YE3232), MtnB (dehydratase, YE3233), MtnE (transaminase, YE3234), and MtnU (possible regulator, YE3235). In addition, there is a second unlinked locus encoding a nuclease (MtnN, YE0739). Therefore, the Y. enterocolitica methionine salvage pathway is similar to that of Klebsiella pneumoniae, with a two-stage conversion of MTA into methylthioribose-1-phosphate and a bifunctional MtnC . In Y. pestis, all of the CDSs encoded in the mtnK–mtnU locus are missing (presumably deleted). However, the mtnN gene has been retained in Y. pestis (YPO3384 in strain CO92, YP0301 in strain 91001, and y0802 in strain KIM10+) and remains intact and in the same genetic context as the Y. enterocolitica mtnN gene. It is known that in nutrient-rich environments and in the presence of low concentrations of dioxygen, facultatively anaerobic bacteria, such as Escherichia coli, simply convert MTA into methylthioribose, using MtnN, and excrete it from the cell. This is likely to be the case for Y. pestis, too, since growth outside of the nutrient-rich environment of the host is unnecessary for its current lifestyle.
OPG is an important constituent of the outer membrane in many Proteobacteria. It was originally identified as being involved in osmoprotection . However, the function of OPG is more complex, since OPG mutants are highly pleiotropic, with defects in virulence, biofilm formation, resistance to antibiotics, and a hypersensitivity to bile salts. The Y. enterocolitica opg cluster is composed of mdoC, mdoG, and mdoH (YE1604–YE1606, respectively). Orthologues of all the opg genes are present in Y. pseudotuberculosis (YPTB2493–YPTB2495) , but mdoC carries multiple nonsense mutations. This entire opg cluster is absent from Y. pestis and is thought to have been deleted, although no detectable remnants remain.
The loss of the OPG cluster by Y. pestis, and its retention by the two enteropathogenic Yersinia, suggests that it remains important for their enteric lifestyle. However, although Y. pseudotuberculosis maintains these CDSs, the loss of a functional mdoC gene suggests that the Y. pseudotuberculosis OPG is nonsuccinylated, and so its function may differ from that of Y. enterocolitica.
Two other complete Y. enterocolitica metabolic pathways have apparently been lost from Y. pseudotuberculosis and Y. pestis, leaving deletion scars behind. These include the cellulose (cel) biosynthetic operon (YE4072–YE4078), which is highly similar in gene content and sequence to that carried by most Salmonella. The only remaining cel CDS in Y. pseudotuberculosis and Y. pestis is bcsZ, encoding endo-1,4-β-glucanase. Although bcsZ appears intact in Y. pseudotuberculosis (YPTB3837), the Y. pestis bcsZ orthologue carries a frameshift mutation. An identical mutation is present in the bcsZ genes in all of the sequenced Y. pestis isolates.
Cellulose production by bacteria is also associated with protection from chemical, as well as mechanical, stress . In Salmonella, the cellulose biosynthetic operon is thought to constitute a transferable module that was acquired by an enterobacterial ancestor as well as a range of other unrelated bacteria . Salmonella produce cellulose in concert with thin aggregative fimbriae to form an inert and highly hydrophobic extracellular matrix. It has been suggested that the protection afforded by this matrix increases retention time of the bacterium in the gut and so offsets the high-energy cost incurred in its production . Cellulose production is presumably redundant for Y. pestis in its new lifestyle. However, why this operon should have been lost by Y. pseudotuberculosis is not as clear. It may reflect niche differences within the enteric environment between the two enteropathogenic Yersinia species, such as the length of time these bacteria reside extracellularly exposed in the gut.
The other pathway deleted from the Y. pseudotuberculosis lineage is tetrathionate respiration. The ability to respire the sulphur-containing compound tetrathionate is used as an identifying trait for Y. enterocolitica  and is facilitated by the tetrathionate reductase–gene cluster (ttr, YE1613–YE1617). The ttr genes appear to have been completely lost from Y. pestis apart from a remnant (identical in all isolates) of ttrR (encoding a two-component regulator governing the tetrathionate operon). All of the ttr genes are missing from Y. pseudotuberculosis. The retention of the complete ttr cluster by Y. enterocolitica is interesting because, uniquely amongst the Yersinia, Y. enterocolitica possess the coenzyme B12–biosynthetic (cbi) and 1,2-propanediol-degradation (pdu) gene clusters located on a single 40-kb genomic island (YE2707–YE2750) [30,31]. This island is inserted between genes that are adjacent in Y. pestis and Y. pseudotuberculosis. Coenzyme B12 is known to be produced only under anaerobic conditions  and is essential for the degradation of 1,2-propanediol as a source of carbon and energy [30,31].
Salmonella possesses cbi, pdu, and ttr gene clusters that are highly related to those of Y. enterocolitica. Like Y. enterocolitica, Salmonella only produces endogenous B12 anaerobically and under those conditions the energetically efficient anaerobic degradation of 1,2-propanediol proceeds with tetrathionate acting as a terminal electron acceptor facilitated by the gene products of the ttr genes . This is likely to also be true for Y. enterocolitica and may therefore explain why the ttr operon has been retained in this species. As has been proposed for Salmonella , this also suggests that 1,2-propanediol is an important source of energy for Y. enterocolitica (and not Y. pseudotuberculosis). The horizontal transfer of the cob/pdu operon has previously been noted as a feature in Salmonella and E. coli divergent evolution [32,33].
In addition to revealing the loss of complete biochemical pathways, the Y. enterocolitica sequence suggests more subtle examples of loss of function in Y. pestis. All the pathogenic yersiniae possess a cluster of 13 CDSs on a genomic island displaying a lower G + C content (35%; compared with genome average of 47%) that we have denoted as Yersinia Genomic Island 1 (YGI-1, YE3632–YE3644 [Figure 1]). YGI-1 is highly related in sequence and gene content to a family of genomic islands, denoted tad loci (tight adherence), present in diverse bacterial and archaeal species, including Actinobacillus actinomycetemcomitans, Pyrococcus abyssi, and Y. pestis [34,35]. The tad locus of A. actinomycetemcomitans, a human pathogen causing endocarditis and periodontitis, has been shown to be important for virulence by encoding the biosynthesis and transport of pili involved in tight, nonspecific adherence [34,36]. In Y. pestis, it has been speculated that the tad genes are important for the colonisation of the flea . However, our data makes this hypothesis unlikely. A comparison of all the Yersinia YGI-1 islands shows that whilst these regions are intact in Y. enterocolitica and Y. pseudotuberculosis, the Y. pestis YGI-1 gene cluster has been truncated by the insertion of IS1541 elements that have resulted in the deletion of the essential pilin gene, flp. Furthermore, all of the sequenced Y. pestis isolates carry an identical frameshift mutation in rcpA (OutD-like type II secretion protein; YPO0692 in strain CO92, YP3007 in strain 91001, and Y3485 in strain KIM10+), predicted to ablate function. This suggests that the loss of this phenotype occurred only once and soon after Y. pestis and Y. pseudotuberculosis diverged. Moreover, since it is predicted that the Tad pilus would be exposed on the surface of the cell, like the loss of YadA , this may be another example of a key mutational event that was selected for by the change in lifestyle of Y. pestis. Consequently, far from being an adaptation to life within the flea, this cluster is likely to be important for enteropathogenicity, explaining why YGI-1 remains intact in Y. enterocolitica and Y. pseudotuberculosis.
Y. enterocolitica Unique CDSs: Functions Acquired Since the Divergence of the Species
Orthologue searches revealed that more than one quarter of the Y. enterocolitica CDSs are absent from the other sequenced Yersinia species. If these CDSs are viewed in the context of the genome, it is evident that many are found in clusters ranging from ∼2–200 kb (Figure 1) and fall into a range of functional categories (Figure 2). Collectively, these species-specific loci contribute to virulence (plasticity zone [PZ]) and significantly broaden the metabolic capability (the hydrogenase operons and cobalamin and propanediol gene clusters, discussed above) of Y. enterocolitica, and consequently may provide clues as to how Y. enterocolitica adapted to its current niche (discussed below).
Plasticity zone: A key locus for high pathogenicity.
The PZ is the largest region of species-specific genomic variation found within the Y. enterocolitica genome. It is bounded on one side by a tRNA-phe gene and accounts for ∼16% of the Y. enterocolitica unique CDSs (an ∼199 kb locus extending from 3,761,922–3,960,673 bps and encoding 186 CDSs [Figure 1]). The PZ is unlikely to have been acquired during a single event and is more likely to have arisen through a series of independent insertions at this site. Several discrete functional units are identifiable within this region, some of which are known to be mobile or sporadically distributed in other bacteria, and some of which are flanked by repeat sequences. These include a region highly similar to the Y. pseudotuberculosis adhesion pathogenicity island (YAPIytb , which we have denoted YAPIye), type III (ysa) and type II (yst1) secretion system clusters, and several metal-uptake operons and resistance-gene loci (Figures 1 and 3).
Microarray analysis of the genomic DNA from 34 Y. enterocolitica isolates, representing five biotypes, constructed using GeneSpring version 6.1 software. Data is presented for the CDS in the range of YE3439–YE3658 including the PZ and YGI-1, as marked (left side). Each numbered column represents the results from a different Y. enterocolitica strain: 1, 09/03; 2, 12/02; 3, 208/02; 4, 35/03; 5, 77/03; 6, 30/02; 7, 81/02; 8, 14/02; 9, 119/02; 10, 212/02; 11, 218/02; 12, 231/02; 13, 56/03; 14, 16/03; 15, 209/02; 16, 149/02; 17, 177/02; 18, 153/02; 19, 202/02; 20, 7/03; 21, 135/02; 22, 8/03; 23, 190/02; 24, 220/02; 25, 227/02; 26, 201/02; 27, Y30; 28, Y73; 29, Y89; 30, Y71; 31, Y68; 32, Y70; 33, Y69; and 34, 8081 (control). See Table S2 for details. The colour-coded biotype key for each isolate is shown at the bottom. Each row represents an individual gene within this region. Coloured blocks (right side) have been used to highlight groups of CDSs showing differing distributions between isolates. The range of CDSs encoded within these blocks is shown (in brackets). Also marked are the relative positions of interesting CDSs or loci that have been mentioned within the body of this article. Blue CDSs correspond to those genes that are considered absent/divergent, and yellow CDSs correspond to genes that are assigned present/conserved. Grey indicates data not obtained.
Within the plasticity zone: YAPIye.
The Y. enterocolitica YAPIye is located between 3,761,992–3,828,092 bps and is flanked by an intact and partial copy of the tRNAphe gene, associated with the integration of this element into this site. In Y. pseudotuberculosis, YAPIytb encodes a type IV pilus operon shown to be important for virulence . YAPIye (66 kb) is significantly smaller than YAPIytb (98 kb ), with a conserved core carrying the type IV pilus operon and encoding plasmid-related functions, as well as a variable region. The variable portion of YAPIytb is predicted to encode various metabolic functions and a type I restriction/modification system , whereas this region of YAPIye encodes a possible hemolysin (YE3454), a toxin/antitoxin system (YE3480 and YE3481), and an arsenic-resistance operon (YE3472–YE3475). Both the arsenic-resistance operon and the type IV pilus cluster are highly similar to those on the S. typhimurium plasmid R64. Arsenic resistance appears to be important for Y. enterocolitica strain 8081 since there is a second chromosomal arsenic-resistance operon (YE3364–YE3366) outside of the PZ, similar to the chromosomally encoded E. coli arsRBC operon , and a different transposon-borne arsenic-resistance operon carried on pYV has been reported from low virulence European strains of Y. enterocolitica . Selection for arsenic resistance in Y. enterocolitica is believed to reflect intensive treatments of pigs with arsenical compounds in the pre-antibiotic era to protect them from diarrhoea caused by Serpulina hyodysenteriae .
The Yersinia YAPI islands share extensive similarity in sequence, gene content, and gene arrangement with the S. typhi pathogenicity island, SPI-7 [41–43], as well as a broader family of genomic islands found in a diverse set of bacteria [41,44,45].
Within the plasticity zone: Secretion systems.
In addition to the Yop type three secretion system (TTSS) encoded on pYV, the Y. enterocolitica PZ carries a second TTSS, denoted as Ysa [46,47]. The Y. enterocolitica ysa operon is composed of 32 CDSs (YE3533 [acpY]–YE3561[ysrS]) and is known to be important for pathogenicity, as ysa mutants show a reduced virulence phenotype .
The PZ also encodes (YE3564–YE3575), a general secretion pathway (GSP)–like system, denoted as Yst1 . Like Ysa, mutants defective for the Yst1-secretion system were found to be impaired in colonisation when introduced by the oral route of infection . In addition, Y. enterocolitica 8081 possesses a second GSP cluster, denoted as Yst2 (Figure 1) , which is located outside of the PZ region and is common to both Y. pestis and Y. pseudotuberculosis.
Within the plasticity zone: Niche adaptation.
The Y. enterocolitica PZ also carries several other gene clusters capable of conferring survival benefits in the gut or wider environment. These include the hydrogenase 2 biosynthetic operon (discussed below), an orthologue of the gene encoding the betaine/proline transporter, ProP (YE3594), a bifunctional protein with roles in both osmoprotection and osmoregulation, and a chitinase (YE3576) that could be secreted by Yst1 . Other CDSs involved in metal uptake and resistance are present in this region. These include the ferric enterochelin operon fepBDGC fes and fepA (YE3618–YE3624 and ; note that fepA is a pseudogene, a system highly similar to the ferrichrome transport system, fhu (YE3583–YE3586), from Bacillus subtilis, YE3629 and YE3630, which are predicted to encode proteins similar to the E. coli silver and copper transporting efflux system, CusA and CusB . Also noteworthy is YE3631, which encodes a product highly similar to the E. coli AlkB protein, which confers resistance to DNA-alkylating agents .
Gene loss is also evident in the PZ. Remnants of an ancestral enteric flagella cluster termed flgII  are present: YE3610 (lipoprotein), YE3610A (flagella protein, pseudogene), YE3611 (regulator [pseudogene]), and YE3614A (flagella regulator [pseudogene]) are orthologues of CDSs found bordering or within the Y. pestis flagella cluster II. The Y. enterocolitica flagella cluster I (2,711,620–2,787,043 bps) remains intact and is known to be functional and important for virulence . However, Y. pestis, which, unlike Y. enterocolitica, is nonmotile, has retained both ancestral flagella clusters, albeit with some degeneracy .
Y. enterocolitica hydrogenase loci: Colonisation of the gut.
The ability to exploit locally generated hydrogen as a source of energy has been recently shown to be essential for colonisation of the gut, and for the virulence of enteric bacteria such as Salmonella and Helicobacter [53–55]. The Y. enterocolitica unique gene-set encodes two [NiFe]-containing hydrogenase complexes, Hyd-4 and Hyd-2, encoded within the hyf locus (YE2796–YE2812, encoding orthologues of E. coli genes hypCA, hyfABCDEFGHIJK, hydN, fdfH, hyfR, and focB) and the hyb locus (YE3600–YE3609, encoding orthologues of E. coli hypFED, hybG, hypB, hybFEDCBAO), respectively.
In E. coli, Hyd-2 acts in a respiratory capacity through the oxidation of molecular hydrogen . Hyd-4 forms a complex with formate dehydrogenase H (Fdh-H), constituting formate hydrogen-lyase system 2 (Fhl-2). Three subunits of Hyd-4 (hyfDEF) are thought to facilitate the translocation of protons across the cytoplasmic membrane , thereby generating a proton gradient that can then be used to generate energy, mainly used to take up amino acids for more rapid growth (for a review see ).
The two Y. enterocolitica hydrogenase clusters are extremely compact, encoding all of the CDSs essential for the functioning and maturation of Hyd-4 and Hyd-2. This is not true of other enteric bacteria described to date, in which these functions are distributed over several different hydrogenase clusters and/or are dispersed throughout the genome. There is no evidence of the hyf and hyb loci in the Y. pestis and Y. pseudotuberculosis genomes. Coupled with their compact nature, this may suggest that they have been acquired by Y. enterocolitica, despite the absence of any obvious mobility genes in these clusters.
Prophage and other regions of difference.
As noted in the genomes of most other sequenced enteric bacteria, much of the Y. enterocolitica novel DNA is composed of prophage-like elements ([59,60]; Figure 1; locations 981,223–1,011,295, 1,849,792–1,887,236, 1,991,720–2,007,210, and 2,503,099–2,554,665, denoted as ΦYE98, ΦYE185, ΦYE200, and ΦYE250, respectively). All of the Y. enterocolitica prophage carry what appear to be “cargo genes,” which are not essential for phage replication but potentially functional in a lysogenic phase. Prophage cargo genes are involved in DNA methylation and regulation, as well as in restriction and modification; the restriction enzyme YenI (YE1808)  lies within a low G + C region of ΦYE200. Interestingly, considering the niche differences and diversity of prophage, Y. pestis carries a prophage that is highly related to ΦYE250 and Y. pseudotuberculosis carries prophage regions highly similar to ΦYE98 and ΦYE185 (Figure 1). These are not present in the same chromosomal context and are likely to be independent acquisitions.
In Y. pestis, the prophage resembling ΦYE250 (DNA identity 80%–90%) has been linked to the presence of noncoding chromosomal regions of clustered regularly interspaced short palindromic repeats (CRISPR loci, comprising direct repeats, from 21 to 37 bp, interspersed with similarly sized nonrepetitive sequences or spacers), also found in Y. pseudotuberculosis . Most of the spacer sequences are thought to have been actively captured from this prophage by an unknown mechanism, and the CRISPR locus is thought to represent a defence system against bacteriophage . Interestingly, four of the 31 described spacer sequences from the three Y. pestis CRISPR loci  are also present in the Y. enterocolitica prophage ΦYE250. Using a standard CRISPR detection method , we have not found a CRISPR locus in the nucleotide sequence of Y. enterocolitica 8081. Specific CRISPR-associated (Cas) proteins  corresponding to the Y. pestis genes YPO2462–8 are not present in Y. enterocolitica. Therefore, either the Y. pestis–Y. enterocolitica common ancestor possessed CRISPR loci lost in Y. enterocolitica 8081 evolution, or an active process has been occurring in Y. pseudotuberculosis and Y. pestis following acquisition of a CRISPR progenitor .
There are several other genomic loci that show a phylogenetically restricted distribution. These include a novel locus, composed of 13 CDSs (YE0894–YE0912), which we have denoted Yersinia genomic island 2 (YGI-2) (see Figure 1). YGI-2 is highly conserved as a genomic island in a wide range of Enterobacteriaceae, including the phytopathogen Erwinia carotovora subsp. atroseptica , enterohaemorrhagic E. coli 0157:H7 , and uropathogenic E. coli CFT073 , as well as in the probiotic E. coli strain Nissle . Notably, this island is missing in E. coli K12 (unpublished data).
YGI-2 has a low G + C content (44.62 %) and is located alongside a tRNAasp gene, characteristic of horizontal gene transfer, although there are no obvious mobility functions encoded on this island. The CDSs within this cluster appear to encode the biosynthesis, modification, and export of an outer membrane anchored glycolipoprotein, the function of which is unclear.
Additionally, there are several other notable genomic loci in this category that carry CDSs predicted to encode an RTX-toxin; an adhesin; sugar-, iron-, and zinc-uptake systems; fimbriae; and two loci that resemble integrated plasmids (see Table 2). Both of the putative integrated plasmids have an atypical G + C content; the first is inserted alongside the stable RNA ssrA gene (tmRNA, denoted as YGI-3, located 1097155–1116114 bps) and flanked by 14 bp direct repeats and the second element (denoted as YGI-4, located at 1308551–1323148 bps, see Figure 1 and Table 2) has inserted into YE1169, leaving an intact copy on one side and a partially duplicated copy (YE1184) on the other side of the element.
Microarray analysis of Y. enterocolitica biotype–specific variation.
Considering the range of different Y. enterocolitica biotypes and the differences they display in their pathogenicity, it was important to define those Y. enterocolitica strain 8081 genetic functions that are characteristic of the species as a whole and those that are strain- or biotype-specific. Microarray data for the genomic DNA of 34 Y. enterocolitica isolates, including 26 UK isolates of biotypes 1A, 2, 3, and 4 and eight US isolates of biotype 1B (including 8081), were used in this analysis and represented a subset of data taken from a much larger phylogenomic study  using a microarray based on Y. enterocolitica strain 8081 (This data is summarised in Figures 1 and 3).
The microarray data confirmed that several of the important metabolic regions detailed above were present in all biotypes tested and so are likely to represent key factors for niche adaptation by this enteropathogen. These include the two hydrogenase gene clusters (hyb and hyf), the cobalamin synthesis (cob) and propanediol utilisation operons (pdu), the gene cluster encoding cellulose biosynthesis (cel), tetrathionate respiration (ttr), and the OPG cluster (opg).
The most obvious biotype-specific regions shown by the Y. enterocolitica 8081 microarray were the four prophages. None of the prophage genes were conserved in the non- (biotype 1A) or mildly pathogenic (biotypes 2, 3, and 4) Y. enterocolitica (Figure 1). In contrast, the degenerate prophage, ΦY200, was fully represented in all 1B isolates except Y69 and Y70, where it was partially detected (unpublished data). Prophage sequences highly related to ΦYE98 were present in biotype 1B isolates Y69 and Y89, and Y71 harboured most genes from ΦYE185. Prophage ΦYE250 was unique to strain 8081 and is likely to be a recent acquisition, perhaps explaining the absence of a CRISPR locus, as discussed above.
The largest single Y. enterocolitica strain 8081–specific locus, seen through whole genome sequence comparisons with other yersiniae, was the PZ. In addition to showing species specificity, microarray analysis revealed that the PZ also showed a marked biotype-specific distribution, consistent with it being a region of hypervariability (Figure 3). Moreover, it was notable that the different subregions of the PZ showed clear biotype delineations, making it suitable for a PCR-based typing scheme.
Two regions within the PZ were common to all of the Y. enterocolitica isolates. The first region encoded the hydrogenase 2 cluster (hyb) and a second locus is predicted to encode SpeF and PotE, which are involved in polyamine uptake in other bacteria. YAPIye, the TTSS ysa, and GSP yst1 were all restricted to highly pathogenic 1B biotypes, consistent with previous findings [17,38,47]. Interestingly, we only detected the presence of YAPIye in one other Y. enterocolitica 1B isolate (Y69) in addition to 8081, and in this instance only CDSs predicted to encode the type IV pilus were present. The YAPI type IV pili genes lie within the core region of this family of mobile genetic elements , suggesting that a distinct YAPIye element with a different gene complement from that in 8081 may exist in this strain.
Also within the PZ, the ferric enterochelin operon was detected in all biotypes tested, except for strains of biotype 4 (consistent with previous results) [17,47,48], and CDSs YE3624–YE3630, which are predicted to encode several metal-resistance functions, were restricted to biotypes 1A and 1B (see Figure 3).
Notable biotype-specific regions outside of the PZ included genomic islands YGI-1 (tad genes), and YGI-2. YGI-2 was detected in biotypes 1A and 1B only (Figure 1), whilst YGI-1 was restricted to the pathogenic Y. enterocolitica biotypes (1B and 2–4; Figure 3). Since YGI-1 is present in all of the other Y. enterocolitica biotypes, and indeed the other pathogenic Yersinia species, it reinforces the view that this locus is important for enteropathogenicity and suggests that it has been lost from the biotype 1A lineage.
Using the microarray data, we determined that there were 992 CDSs present in Y. enterocolitica strain 8081 (biotype 1B) that were not detected in the biotypes 1A, 2, 3, and 4 isolates tested (Figure 1). Within this gene set, 406 CDSs were represented in all the other members of biotype 1B tested. Furthermore, 119 CDSs were unique to Y. enterocolitica strain 8081, as they were not detected in any of the other Y. enterocolitica isolates tested by microarray (Listed in Table S3).
Consistent with previous results, the biotype 1B–specific CDSs included the CDSs within the high-pathogenicity island and several of the regions located within the PZ, as discussed above. Other virulence-associated functions in this group include the Serratia marcescens HlyA-like hemolysin and activator (YE2407 and YE2408, also present in Y. pseudotuberculosis and Y. pestis) an autotransporter (YE1372), a serine protease (YE1389), and a putative TTSS effector protein (YE2447) that is highly similar (91% amino acid–sequence identity) to the Shigella flexneri TTSS effector protein OspG, which in S. flexneri is a protein kinase that has been shown to interfere with the innate immune response . The arsenic-resistance operon (YE3364–YE3366) located outside of the PZ is also restricted to biotype 1B isolates. Interestingly, the integrated plasmid region (YGI-4) is variably present in several other 1B isolates (Y69 and Y30, unpublished data).
Of the CDSs that were found by microarray analysis to be unique to the sequenced strain 8081, the majority (104/119) were clustered, constituting ΦYE250, one of the two proposed integrated plasmid regions: YGI-3, the putative hemolysin (YE3454), and the variable portion of the YAPIye, encoding the arsenic-resistance operon (Figure 3). It is likely that these elements represent the most recent acquisition events in this strain and this underlines the fact that lateral gene transfer continues to be an important source of new genetic material within the yersiniae.
The genome of Y. enterocolitica and its comparison with the genomes of Y. pseudotuberculosis and Y. pestis reveal fascinating insights into gene loss and acquisition that have occurred since these yersiniae diverged. We identified Y. enterocolitica–specific genes, some of which showed evidence of previous loss from both Y. pestis and Y. pseudotuberculosis. We also identified loci that were putative enteropathogenic yersinia–specific genes retained by Y. enterocolitica and Y. pseudotuberculosis but lost by Y. pestis (Table 2).
The core set of genes encoding orthologous proteins shared by Y. enterocolitica strain 8081, Y. pestis strain CO92, and Y. pseudotuberculosis strain IP32953, defined in this study by reciprocal FastA analysis (2,747 CDSs), is much higher than the number of core genes detected in all isolates of Y. enterocolitica by comparative genome hybridisation (894 CDSs) . This can be explained either by a higher level of variation found within the Y. enterocolitica strains compared with that seen within Y. pestis and Y. pseudotuberculosis , or, more likely, that the number observed in  represents measurement of gene divergence rather than complete gene loss and so is an underestimation due to the constraints of comparative genome hybridisation analysis.
Microarray data was instrumental in identifying which of the metabolic functions identified from the sequenced strain could be considered core Y. enterocolitica functions. These data was then used to strengthen the comparison of the metabolic capabilities identified in the genome sequence of Y. enterocolitica strain 8081 with those of the other sequenced pathogenic Yersinia sp., identifying significant metabolic pathway differences.
Metabolic pathway defects long recognised in Y. pestis compared with Y. pseudotuberculosis  have suggested that there is a change in Y. pestis metabolism, triggered by the temperature difference between the flea and mammalian host. From the perspective of enteropathogenic yersiniae, we can identify another pathway that has been lost from Y. pestis, involving methionine salvage, correlating with its amino acid–rich blood environment. Methionine salvage–pathway enzymes can produce carbon monoxide , a molecule capable of affecting host gut signalling pathways , so there may be an additional nonnutritional advantage for this pathway in enteric pathogens. The presence of this pathway from the perspective of the enteropathogenic Yersinia is also interesting because it may present a target for antimicrobial chemotherapy .
The loss of function in Y. pestis of many genes associated with enteric pathogenicity is widely accepted, but rather more surprising was the apparent loss by Y. pseudotuberculosis of several presumptive enteric adaptation functions maintained in Y. enterocolitica. These include the OPG and cellulose biosynthetic genes and the differences in polyamine uptake and metabolism. All these functions are associated with protection from physical and chemical stress, and their loss therefore suggests that Y. enterocolitica occupies a significantly different niche than Y. pseudotuberculosis, which is more exposed to the conditions experienced within the gut lumen, and is perhaps associated with a longer retention time. Y. enterocolitica gut colonisation of apparently healthy animals (particularly pigs) at slaughter is well-recognised , and more prolonged excretion of Y. enterocolitica as compared with Y. pseudotuberculosis following infection in animals has been noted in vivo .
Competition for essential nutrients is increasingly recognised to be a survival strategy for pathogens . Further metabolic evidence for Y. enterocolitica and Y. pseudotuberculosis occupying different niches while both being enteric pathogens is provided by the Y. enterocolitica–specific hydrogenase clusters. The two Y. enterocolitica [NiFe] hydrogenase operons are absent from the sequenced Y. pestis and Y. pseudotuberculosis genomes and appear as clear insertions into Y. enterocolitica. Furthermore, the notably compact arrangement of these clusters and the microarray data showing that all biotypes of Y. enterocolitica possess these genes suggest that they were acquired by lateral transfer at a point soon after speciation. H2 is abundant in the intestines and deeper tissues of animals and humans, a product of fermentative growth by colonic bacteria [54,55,75]. Since it has been shown that the ability to use H2 as an energy source for some enteric bacteria is central to their ability to colonise the gut and ultimately to cause disease [53,54], it is intriguing to consider why these functions are apparently unimportant for Y. pseudotuberculosis, also primarily a faecal–oral pathogen, and what that may suggest about differing disease processes in Y. enterocolitica and Y. pseudotuberculosis.
Wider comparisons with other members of the enterobacteriaceae have highlighted interesting parallels in their evolution. Like Y. pestis, S. typhi has become an acute systemic pathogen whilst its relatives, such as S. typhimurium, have remained essentially as enteropathogens. It is apparent that like Yersinia, Salmonella diversity is being driven by phage integration, plasmid acquisition (both integrated and extrachromosomal), and pseudogene formation (and gene deletion), as well as through the introduction of novel DNA through flexible loci, such as tRNA genes. In addition to these general themes, there are some more specific overlaps. It has been previously shown that the S. typhi plasmid, pHCM2, is highly related to the Y. pestis plasmid, pMT1 , and it is thought that the pathogenicity island, SPI-7 (encoding the major virulence antigen genes), and the important Yersinia YAPI loci [41,76] were derived from a common ancestor . This suggests DNA exchange or that Salmonella and Yersinia have shared a common gene pool.
There is also similar evidence of metabolic “streamlining” in Salmonella. We have highlighted several functions that have been lost by one or more of the yersiniae, including the Y. enterocolitica–specific ttr cluster and cob/pdu operons, conferring the ability to use completely different energy supplies in and around the gut , as well as the cellulose biosynthetic cluster (discussed above). Similar observations can be made when comparing S. typhi with S. typhimurium. For example, the cob/pdu, ttr, and the cel gene clusters all carry multiple pseudogenes in S. typhi, yet have all been maintained apparently intact by S. typhimurium. Other similarities relate to hydrogenase gene clusters. In S. typhi, there is evidence of gene loss within hydrogenase clusters, with pseudogenes present in the hya (hydrogenase 1) and the membrane-bound hydrogenase gene cluster.
These data imply that members of the yersiniae and salmonellae have found common solutions to niche adaptation by gene acquisition and loss, perhaps even occupying similar metabolic niches. Moreover, as in S. typhi and S. typhimurium, although Y. enterocolitica and Y. pseudotuberculosis are both enteric pathogens, localisation and dynamics of Y. enterocolitica infection, we predict, in terms of site and rate of maximal growth in the host, are significantly different from Y. pseudotuberculosis.
Materials and Methods
We chose to sequence a human septicaemia isolate, Y. enterocolitica strain 8081 . 8081 is the prototype Y. enterocolitica strain that has been used extensively in the murine yersiniosis infection model to study gastrointestinal host–pathogen interactions and has been developed as an effective oral vaccine delivery system [78–80]. A single colony of Y. enterocolitica strain 8081 was picked from Congo Red agar and grown overnight in BAB broth with shaking at 30 °C. Cells were collected and total DNA (10 mg) was isolated using proteinase K treatment followed by phenol extraction. The DNA was fragmented by sonication, and several libraries were generated in pUC18 using size fractions ranging from 1.0 to 2.5 kb. The whole genome was sequenced to a depth of 9× coverage from M13mp18 (insert size 1.4–2 kb) and pUC18 (insert size 2.2–4.2 kb) small-insert libraries using dye-terminator chemistry on ABI3700 automated sequencers. End sequences from larger insert plasmid (pBACe3.6, 12–30 kb insert size) libraries were used as a scaffold.
The genome sequences of Y. enterocolitica, Y. pestis strain CO92, Y. pestis strain KIM10+, Y. pestis strain 91001, and Y. pseudotuberculosis strain IP32953 were compared pairwise using the Artemis Comparison Tool . Pseudogenes had one or more mutations that would ablate expression; each of the inactivating mutations was subsequently checked against the original sequencing data.
The pYVe8081 virulence plasmid (67,721 bp) was also sequenced as part of the genomic shotgun. The sequence of this plasmid was found to be identical to that previously sequenced , apart from a single-base insertion and nine single-nucleotide differences, seven of which were synonymous or located in noncoding regions. The nonsynonymous mutations were found in YEP0063 (hypothetical protein, Phe–Leu substitution) and YEP0069 (transposase, Phe–Ser substitution) (unpublished data).
The genome has been submitted to the EMBL public database (10.1371/journal.pgen.0020206_01). Accession numbers are listed in the Supporting Information section below. The genome submission is MIGS compliant (10.1371/journal.pgen.0020206_02, GCAT identifier 000001_GCAT). This strain has been deposited as NCTC 13174.
Generating orthologous gene sets.
Orthologous gene sets were identified by reciprocal FASTA searches. Only those pairs of homologous CDSs were retained for further analysis where the predicted amino acid identity was ≥40% over 80% of the protein length. These genes were then subjected to manual curation using gene synteny to increase the accuracy of this analysis. This strategy was applied to pairwise comparisons of the genomes of Y. enterocolitica strain 8081, Y. pestis (strains CO92, 91001, and KIM10+), and Y. pseudotuberculosis strain IP32953.
The microarray was designed to include all 4,036 predicted CDSs from the Y. enterocolitica 8081 genome as previously described . The strains and raw microarray data used in this study was derived from a much larger phylogenomic study using a microarray based on Y. enterocolitica strain 8081 . Data was processed and genes designated as present, divergent, or absent (highly divergent) as previously described . Table S2 details the strains used in this study. All strains were identified using standard biochemical typing tests as previously described . Microarray data can be found in Array Express (http://www.ebi.ac.uk/arrayexpress) in Supporting Information below.
Table S1. Insertion Sequence Elements Found within the Sequenced Yersinia Genomes
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Table S2. Strain List of the Y. enterocolitica Isolates Used in the Microarray Analysis
(92 KB DOC)
Table S3. CDS Unique to Y. enterocolitica Strain 8081 as Defined by Comparative Microarray Analysis of 33 Other Isolates of Biotypes 1B, 1A, 2, 3, and 4
(224 KB DOC)
The EMBL (10.1371/journal.pgen.0020206_01) accession numbers for the genome of Y. enterocolitica strain 8081are AM286415 and AM286416 (plasmid).
The Array Express (10.1371/journal.pgen.0020206_03) accession number for the Y. enterocolitica strain 8081 microarray data is E-BUGS-36. Y. enterocolitica strain 8081 was deposited with the Health Protection Agency (United Kingdom, http://www.hpa.org.uk/nctc/searcher.html) as NCTC 13174.
We thank the core sequencing and informatics teams at the Sanger Institute for their assistance and The Wellcome Trust for its support of the Sanger Institute Pathogen Sequencing Unit. We thank the Bacterial Microarray Group at St. George's Hospital, University of London, for provision of the Y. enterocolitica strain 8081 microarray. We thank Daniel Portnoy for the gift of Y. entercolitica 8081.
NRT, SH, BWW, JP and MBP conceived and designed the experiments. NRT, SH, CC, KM, KB, TC, TF, ZA, HH, KJ, MM, SM, MS, SW, MAQ, and MBP performed the experiments. NRT, SH, BWW, MTGH, LC, GLC, GD, JP, and MBP analyzed the data. NRT SH, BWW, GLC, and MBP wrote the paper.
- 1. Ljungberg P, Valtonen M, Harjola VP, Kaukoranta-Tolvanen SS, Vaara M (1995) Report of four cases of Yersinia pseudotuberculosis septicemia and a literature review. Eur J Clin Microbiol Infect Dis 14: 804–810.P. LjungbergM. ValtonenVP HarjolaSS Kaukoranta-TolvanenM. Vaara1995Report of four cases of Yersinia pseudotuberculosis septicemia and a literature review.Eur J Clin Microbiol Infect Dis14804810
- 2. Perry RD, Fetherston JD (1997) Yersinia pestis—Etiologic agent of plague. Clin Microbiol Rev 10: 35–66.RD PerryJD Fetherston1997Yersinia pestis—Etiologic agent of plague.Clin Microbiol Rev103566
- 3. Achtman M, Morelli G, Zhu P, Wirth T, Diehl I, et al. (2004) Microevolution and history of the plague bacillus, Yersinia pestis. Proc Natl Acad Sci U S A 101: 17837–17842.M. AchtmanG. MorelliP. ZhuT. WirthI. Diehl2004Microevolution and history of the plague bacillus, Yersinia pestis.Proc Natl Acad Sci U S A1011783717842
- 4. Achtman M, Zurth K, Morelli G, Torrea G, Guiyoule A, et al. (1999) Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis. Proc Natl Acad Sci U S A 96: 14043–14048.M. AchtmanK. ZurthG. MorelliG. TorreaA. Guiyoule1999Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis.Proc Natl Acad Sci U S A961404314048
- 5. Wren BW (2003) The yersiniae—A model genus to study the rapid evolution of bacterial pathogens. Nat Rev Microbiol 1: 55–64.BW Wren2003The yersiniae—A model genus to study the rapid evolution of bacterial pathogens.Nat Rev Microbiol15564
- 6. Wauters G, Kandolo K, Janssens K (1987) Revised biogrouping scheme of Yersinia enterocolitica. Contrib Microbiol Immunol 9: 14–21.G. WautersK. KandoloK. Janssens1987Revised biogrouping scheme of Yersinia enterocolitica.Contrib Microbiol Immunol91421
- 7. Van Noyen R, Vandepitte J, Wauters G, Selderslaghs R (1981) Yersinia enterocolitica: Its isolation by cold enrichment from patients and healthy subjects. J Clin Pathol 34: 1052–1056.R. Van NoyenJ. VandepitteG. WautersR. Selderslaghs1981Yersinia enterocolitica: Its isolation by cold enrichment from patients and healthy subjects.J Clin Pathol3410521056
- 8. McNally A, Cheasty T, Fearnley C, Dalziel RW, Paiba GA, et al. (2004) Comparison of the biotypes of Yersinia enterocolitica isolated from pigs, cattle and sheep at slaughter and from humans with yersiniosis in Great Britain during 1999–2000. Lett Appl Microbiol 39: 103–108.A. McNallyT. CheastyC. FearnleyRW DalzielGA Paiba2004Comparison of the biotypes of Yersinia enterocolitica isolated from pigs, cattle and sheep at slaughter and from humans with yersiniosis in Great Britain during 1999–2000.Lett Appl Microbiol39103108
- 9. Prentice MB, Cope D, Swann RA (1991) The epidemiology of Yersinia enterocolitica infection in the British Isles 1983–1988. Contrib Microbiol Immunol 12: 17–25.MB PrenticeD. CopeRA Swann1991The epidemiology of Yersinia enterocolitica infection in the British Isles 1983–1988.Contrib Microbiol Immunol121725
- 10. Schubert BA, Wagner NJ, Kaler EW, Raghavan SR (2004) Shear-induced phase separation in solutions of wormlike micelles. Langmuir 20: 3564–3573.BA SchubertNJ WagnerEW KalerSR Raghavan2004Shear-induced phase separation in solutions of wormlike micelles.Langmuir2035643573
- 11. Schubert S, Rakin A, Heesemann J (2004) The Yersinia high-pathogenicity island (HPI): Evolutionary and functional aspects. Int J Med Microbiol 294: 83–94.S. SchubertA. RakinJ. Heesemann2004The Yersinia high-pathogenicity island (HPI): Evolutionary and functional aspects.Int J Med Microbiol2948394
- 12. Brubaker RR (1991) Factors promoting acute and chronic diseases caused by yersiniae. Clin Microbiol Rev 4: 309–324.RR Brubaker1991Factors promoting acute and chronic diseases caused by yersiniae.Clin Microbiol Rev4309324
- 13. Cornelis GR (2002) Yersinia type III secretion: Send in the effectors. J Cell Biol 158: 401–408.GR Cornelis2002Yersinia type III secretion: Send in the effectors.J Cell Biol158401408
- 14. Heesemann J, Laufs R (1984) Genetic manipulation of virulence of Yersinia enterocolitica and Yersinia pseudotuberculosis. Zentralbl Bakteriol Mikrobiol Hyg [A] 256: 416–417.J. HeesemannR. Laufs1984Genetic manipulation of virulence of Yersinia enterocolitica and Yersinia pseudotuberculosis.Zentralbl Bakteriol Mikrobiol Hyg [A]256416417
- 15. Heesemann J, Algermissen B, Laufs R (1984) Genetically manipulated virulence of Yersinia enterocolitica. Infect Immun 46: 105–110.J. HeesemannB. AlgermissenR. Laufs1984Genetically manipulated virulence of Yersinia enterocolitica.Infect Immun46105110
- 16. Heesemann J, Laufs R (1983) Plasmid-mediated antigens of human pathogenic Yersinia enterocolitica strains. Zentralbl Bakteriol Mikrobiol Hyg [A] 253: 428–429.J. HeesemannR. Laufs1983Plasmid-mediated antigens of human pathogenic Yersinia enterocolitica strains.Zentralbl Bakteriol Mikrobiol Hyg [A]253428429
- 17. Iwobi A, Heesemann J, Garcia E, Igwe E, Noelting C, et al. (2003) Novel virulence-associated type II secretion system unique to high-pathogenicity Yersinia enterocolitica. Infect Immun 71: 1872–1879.A. IwobiJ. HeesemannE. GarciaE. IgweC. Noelting2003Novel virulence-associated type II secretion system unique to high-pathogenicity Yersinia enterocolitica.Infect Immun7118721879
- 18. Chain PS, Carniel E, Larimer FW, Lamerdin J, Stoutland PO, et al. (2004) Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis. Proc Natl Acad Sci U S A 101: 13826–13831.PS ChainE. CarnielFW LarimerJ. LamerdinPO Stoutland2004Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis.Proc Natl Acad Sci U S A1011382613831
- 19. Parkhill J, Wren BW, Thomson NR, Titball RW, Holden MT, et al. (2001) Genome sequence of Yersinia pestis, the causative agent of plague. Nature 413: 523–527.J. ParkhillBW WrenNR ThomsonRW TitballMT Holden2001Genome sequence of Yersinia pestis, the causative agent of plague.Nature413523527
- 20. Song Y, Tong Z, Wang J, Wang L, Guo Z, et al. (2004) Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans. DNA Res 11: 179–197.Y. SongZ. TongJ. WangL. WangZ. Guo2004Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans.DNA Res11179197
- 21. Deng W, Burland V, Plunkett G III, Boutin A, Mayhew GF, et al. (2002) Genome sequence of Yersinia pestis KIM. J Bacteriol 184: 4601–4611.W. DengV. BurlandG. Plunkett IIIA. BoutinGF Mayhew2002Genome sequence of Yersinia pestis KIM.J Bacteriol18446014611
- 22. Ochman H, Davalos LM (2006) The nature and dynamics of bacterial genomes. Science. pp. 1730–1733.H. OchmanLM Davalos2006The nature and dynamics of bacterial genomes.Science17301733
- 23. Day WA Jr, Fernandez RE, Maurelli AT (2001) Pathoadaptive mutations that enhance virulence: Genetic organization of the cadA regions of Shigella spp. Infect Immun 69: 7471–7480.WA Day JrRE FernandezAT Maurelli2001Pathoadaptive mutations that enhance virulence: Genetic organization of the cadA regions of Shigella spp.Infect Immun6974717480
- 24. Sekowska A, Denervaud V, Ashida H, Michoud K, Haas D, et al. (2004) Bacterial variations on the methionine salvage pathway. BMC Microbiol 4: 9.A. SekowskaV. DenervaudH. AshidaK. MichoudD. Haas2004Bacterial variations on the methionine salvage pathway.BMC Microbiol49
- 25. Wray JW, Abeles RH (1995) The methionine salvage pathway in Klebsiella pneumoniae and rat liver. Identification and characterization of two novel dioxygenases. J Biol Chem 270: 3147–3153.JW WrayRH Abeles1995The methionine salvage pathway in Klebsiella pneumoniae and rat liver. Identification and characterization of two novel dioxygenases.J Biol Chem27031473153
- 26. Bohin JP (2000) Osmoregulated periplasmic glucans in Proteobacteria. FEMS Microbiol Lett 186: 11–19.JP Bohin2000Osmoregulated periplasmic glucans in Proteobacteria.FEMS Microbiol Lett1861119
- 27. Lacroix JM, Lanfroy E, Cogez V, Lequette Y, Bohin A, et al. (1999) The mdoC gene of Escherichia coli encodes a membrane protein that is required for succinylation of osmoregulated periplasmic glucans. J Bacteriol 181: 3626–3631.JM LacroixE. LanfroyV. CogezY. LequetteA. Bohin1999The mdoC gene of Escherichia coli encodes a membrane protein that is required for succinylation of osmoregulated periplasmic glucans.J Bacteriol18136263631
- 28. Zogaj X, Nimtz M, Rohde M, Bokranz W, Romling U (2001) The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix. Mol Microbiol 39: 1452–1463.X. ZogajM. NimtzM. RohdeW. BokranzU. Romling2001The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix.Mol Microbiol3914521463
- 29. Weissfeld AS, Sonnenwirth AC (1982) Rapid isolation of Yersinia spp. from feces. J Clin Microbiol 15: 508–510.AS WeissfeldAC Sonnenwirth1982Rapid isolation of Yersinia spp. from feces.J Clin Microbiol15508510
- 30. Prentice MB, Cuccui J, Thomson N, Parkhill J, Deery E, et al. (2003) Cobalamin synthesis in Yersinia enterocolitica 8081. Functional aspects of a putative metabolic island. Adv Exp Med Biol 529: 43–46.MB PrenticeJ. CuccuiN. ThomsonJ. ParkhillE. Deery2003Cobalamin synthesis in Yersinia enterocolitica 8081. Functional aspects of a putative metabolic island.Adv Exp Med Biol5294346
- 31. Roth JR, Lawrence JG, Bobik TA (1996) Cobalamin (coenzyme B12): Synthesis and biological significance. Annu Rev Microbiol 50: 137–181.JR RothJG LawrenceTA Bobik1996Cobalamin (coenzyme B12): Synthesis and biological significance.Annu Rev Microbiol50137181
- 32. Porwollik S, Wong RM, McClelland M (2002) Evolutionary genomics of Salmonella: Gene acquisitions revealed by microarray analysis. Proc Natl Acad Sci U S A 99: 8956–8961.S. PorwollikRM WongM. McClelland2002Evolutionary genomics of Salmonella: Gene acquisitions revealed by microarray analysis.Proc Natl Acad Sci U S A9989568961
- 33. Lawrence JG, Roth JR (1996) Evolution of coenzyme B12 synthesis among enteric bacteria: Evidence for loss and reacquisition of a multigene complex. Genetics 142: 11–24.JG LawrenceJR Roth1996Evolution of coenzyme B12 synthesis among enteric bacteria: Evidence for loss and reacquisition of a multigene complex.Genetics1421124
- 34. Schreiner HC, Sinatra K, Kaplan JB, Furgang D, Kachlany SC, et al. (2003) Tight-adherence genes of Actinobacillus actinomycetemcomitans are required for virulence in a rat model. Proc Natl Acad Sci U S A 100: 7295–7300.HC SchreinerK. SinatraJB KaplanD. FurgangSC Kachlany2003Tight-adherence genes of Actinobacillus actinomycetemcomitans are required for virulence in a rat model.Proc Natl Acad Sci U S A10072957300
- 35. Planet PJ, Kachlany SC, Fine DH, DeSalle R, Figurski DH (2003) The widespread colonization island of Actinobacillus actinomycetemcomitans. Nat Genet 34: 193–198.PJ PlanetSC KachlanyDH FineR. DeSalleDH Figurski2003The widespread colonization island of Actinobacillus actinomycetemcomitans.Nat Genet34193198
- 36. Kachlany SC, Planet PJ, Bhattacharjee MK, Kollia E, DeSalle R, et al. (2000) Nonspecific adherence by Actinobacillus actinomycetemcomitans requires genes widespread in bacteria and archaea. J Bacteriol 182: 6169–6176.SC KachlanyPJ PlanetMK BhattacharjeeE. KolliaR. DeSalle2000Nonspecific adherence by Actinobacillus actinomycetemcomitans requires genes widespread in bacteria and archaea.J Bacteriol18261696176
- 37. Rosqvist R, Skurnik M, Wolf-Watz H (1988) Increased virulence of Yersinia pseudotuberculosis by two independent mutations. Nature 334: 522–524.R. RosqvistM. SkurnikH. Wolf-Watz1988Increased virulence of Yersinia pseudotuberculosis by two independent mutations.Nature334522524
- 38. Collyn F, Billault A, Mullet C, Simonet M, Marceau M (2004) YAPI, a new Yersinia pseudotuberculosis pathogenicity island. Infect Immun 72: 4784–4790.F. CollynA. BillaultC. MulletM. SimonetM. Marceau2004YAPI, a new Yersinia pseudotuberculosis pathogenicity island.Infect Immun7247844790
- 39. Carlin A, Shi W, Dey S, Rosen BP (1995) The ars operon of Escherichia coli confers arsenical and antimonial resistance. J Bacteriol 177: 981–986.A. CarlinW. ShiS. DeyBP Rosen1995The ars operon of Escherichia coli confers arsenical and antimonial resistance.J Bacteriol177981986
- 40. Neyt CM, Iriarte M, Thi VH, Cornelis GR (1997) Virulence and arsenic resistance in Yersiniae. J Bacteriol 179: 612–619.CM NeytM. IriarteVH ThiGR Cornelis1997Virulence and arsenic resistance in Yersiniae.J Bacteriol179612619
- 41. Pickard D, Wain J, Baker S, Line A, Chohan S, et al. (2003) Composition, acquisition, and distribution of the Vi exopolysaccharide-encoding Salmonella enterica pathogenicity island SPI-7. J Bacteriol 185: 5055–5065.D. PickardJ. WainS. BakerA. LineS. Chohan2003Composition, acquisition, and distribution of the Vi exopolysaccharide-encoding Salmonella enterica pathogenicity island SPI-7.J Bacteriol18550555065
- 42. Parkhill J, Dougan G, James KD, Thomson NR, Pickard D, et al. (2001) Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18. Nature 413: 848–852.J. ParkhillG. DouganKD JamesNR ThomsonD. Pickard2001Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18.Nature413848852
- 43. Collyn F, Guy L, Marceau M, Simonet M, Roten CA (2006) Describing ancient horizontal gene transfers at the nucleotide and gene levels by comparative pathogenicity island genometrics. Bioinformatics 22: 1072–1079.F. CollynL. GuyM. MarceauM. SimonetCA Roten2006Describing ancient horizontal gene transfers at the nucleotide and gene levels by comparative pathogenicity island genometrics.Bioinformatics2210721079
- 44. Bell KS, Sebaihia M, Pritchard L, Holden MT, Hyman LJ, et al. (2004) Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors. Proc Natl Acad Sci U S A 101: 11105–11110.KS BellM. SebaihiaL. PritchardMT HoldenLJ Hyman2004Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors.Proc Natl Acad Sci U S A1011110511110
- 45. Mohd-Zain Z, Turner SL, Cerdeno-Tarraga AM, Lilley AK, Inzana TJ, et al. (2004) Transferable antibiotic resistance elements in Haemophilus influenzae share a common evolutionary origin with a diverse family of syntenic genomic islands. J Bacteriol 186: 8114–8122.Z. Mohd-ZainSL TurnerAM Cerdeno-TarragaAK LilleyTJ Inzana2004Transferable antibiotic resistance elements in Haemophilus influenzae share a common evolutionary origin with a diverse family of syntenic genomic islands.J Bacteriol18681148122
- 46. Haller JC, Carlson S, Pederson KJ, Pierson DE (2000) A chromosomally encoded type III secretion pathway in Yersinia enterocolitica is important in virulence. Mol Microbiol 36: 1436–1446.JC HallerS. CarlsonKJ PedersonDE Pierson2000A chromosomally encoded type III secretion pathway in Yersinia enterocolitica is important in virulence.Mol Microbiol3614361446
- 47. Foultier B, Troisfontaines P, Muller S, Opperdoes FR, Cornelis GR (2002) Characterization of the ysa pathogenicity locus in the chromosome of Yersinia enterocolitica and phylogeny analysis of type III secretion systems. J Mol Evol 55: 37–51.B. FoultierP. TroisfontainesS. MullerFR OpperdoesGR Cornelis2002Characterization of the ysa pathogenicity locus in the chromosome of Yersinia enterocolitica and phylogeny analysis of type III secretion systems.J Mol Evol553751
- 48. Schubert S, Fischer D, Heesemann J (1999) Ferric enterochelin transport in Yersinia enterocolitica: Molecular and evolutionary aspects. J Bacteriol 181: 6387–6395.S. SchubertD. FischerJ. Heesemann1999Ferric enterochelin transport in Yersinia enterocolitica: Molecular and evolutionary aspects.J Bacteriol18163876395
- 49. Franke S, Grass G, Rensing C, Nies DH (2003) Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli. J Bacteriol 185: 3804–3812.S. FrankeG. GrassC. RensingDH Nies2003Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli.J Bacteriol18538043812
- 50. Chen BJ, Carroll P, Samson L (1994) The Escherichia coli AlkB protein protects human cells against alkylation-induced toxicity. J Bacteriol 176: 6255–6261.BJ ChenP. CarrollL. Samson1994The Escherichia coli AlkB protein protects human cells against alkylation-induced toxicity.J Bacteriol17662556261
- 51. Ren CP, Beatson SA, Parkhill J, Pallen MJ (2005) The Flag-2 locus, an ancestral gene cluster, is potentially associated with a novel flagellar system from Escherichia coli. J Bacteriol 187: 1430–1440.CP RenSA BeatsonJ. ParkhillMJ Pallen2005The Flag-2 locus, an ancestral gene cluster, is potentially associated with a novel flagellar system from Escherichia coli.J Bacteriol18714301440
- 52. Young GM, Badger JL, Miller VL (2000) Motility is required to initiate host cell invasion by Yersinia enterocolitica. Infect Immun 68: 4323–4326.GM YoungJL BadgerVL Miller2000Motility is required to initiate host cell invasion by Yersinia enterocolitica.Infect Immun6843234326
- 53. Maier RJ, Olczak A, Maier S, Soni S, Gunn J (2004) Respiratory hydrogen use by Salmonella enterica serovar Typhimurium is essential for virulence. Infect Immun 72: 6294–6299.RJ MaierA. OlczakS. MaierS. SoniJ. Gunn2004Respiratory hydrogen use by Salmonella enterica serovar Typhimurium is essential for virulence.Infect Immun7262946299
- 54. Olson JW, Maier RJ (2002) Molecular hydrogen as an energy source for Helicobacter pylori. Science 298: 1788–1790.JW OlsonRJ Maier2002Molecular hydrogen as an energy source for Helicobacter pylori.Science29817881790
- 55. Maier RJ (2005) Use of molecular hydrogen as an energy substrate by human pathogenic bacteria. Biochem Soc Trans 33: 83–85.RJ Maier2005Use of molecular hydrogen as an energy substrate by human pathogenic bacteria.Biochem Soc Trans338385
- 56. Menon NK, Chatelus CY, Dervartanian M, Wendt JC, Shanmugam KT, et al. (1994) Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2. J Bacteriol 176: 4416–4423.NK MenonCY ChatelusM. DervartanianJC WendtKT Shanmugam1994Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.J Bacteriol17644164423
- 57. Andrews SC, Berks BC, McClay J, Ambler A, Quail MA, et al. (1997) A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system. Microbiology 143(Part 11): 3633–3647.SC AndrewsBC BerksJ. McClayA. AmblerMA Quail1997A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system.Microbiology143Part 1136333647
- 58. Vignais PM, Colbeau A (2004) Molecular biology of microbial hydrogenases. Curr Issues Mol Biol 6: 159–188.PM VignaisA. Colbeau2004Molecular biology of microbial hydrogenases.Curr Issues Mol Biol6159188
- 59. Thomson N, Baker S, Pickard D, Fookes M, Anjum M, et al. (2004) The role of prophage-like elements in the diversity of Salmonella enterica serovars. J Mol Biol 339: 279–300.N. ThomsonS. BakerD. PickardM. FookesM. Anjum2004The role of prophage-like elements in the diversity of Salmonella enterica serovars.J Mol Biol339279300
- 60. Brussow H, Canchaya C, Hardt WD (2004) Phages and the evolution of bacterial pathogens: From genomic rearrangements to lysogenic conversion. Microbiol Mol Biol Rev 68: 560–602.H. BrussowC. CanchayaWD Hardt2004Phages and the evolution of bacterial pathogens: From genomic rearrangements to lysogenic conversion.Microbiol Mol Biol Rev68560602
- 61. Antonenko V, Pawlow V, Heesemann J, Rakin A (2003) Characterization of a novel unique restriction-modification system from Yersinia enterocolitica O:8 1B. FEMS Microbiol Lett 219: 249–252.V. AntonenkoV. PawlowJ. HeesemannA. Rakin2003Characterization of a novel unique restriction-modification system from Yersinia enterocolitica O:8 1B.FEMS Microbiol Lett219249252
- 62. Pourcel C, Salvignol G, Vergnaud G (2005) CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies. Microbiology 151: 653–663.C. PourcelG. SalvignolG. Vergnaud2005CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies.Microbiology151653663
- 63. Jansen R, Embden JD, Gaastra W, Schouls LM (2002) Identification of genes that are associated with DNA repeats in prokaryotes. Mol Microbiol 43: 1565–1575.R. JansenJD EmbdenW. GaastraLM Schouls2002Identification of genes that are associated with DNA repeats in prokaryotes.Mol Microbiol4315651575
- 64. Haft DH, Selengut J, Mongodin EF, Nelson KE (2005) A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes. PLoS Comput Biol 1(6): e60.. DH HaftJ. SelengutEF MongodinKE Nelson2005A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes.PLoS Comput Biol16e60.
- 65. Godde JS, Bickerton A (2006) The repetitive DNA elements called CRISPRs and their associated genes: Evidence of horizontal transfer among prokaryotes. J Mol Evol 62: 718–729.JS GoddeA. Bickerton2006The repetitive DNA elements called CRISPRs and their associated genes: Evidence of horizontal transfer among prokaryotes.J Mol Evol62718729
- 66. Perna NT, Plunkett G III, Burland V, Mau B, Glasner JD, et al. (2001) Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409: 529–533.NT PernaG. Plunkett IIIV. BurlandB. MauJD Glasner2001Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.Nature409529533
- 67. Welch RA, Burland V, Plunkett G III, Redford P, Roesch P, et al. (2002) Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli. Proc Natl Acad Sci U S A 99: 17020–17024.RA WelchV. BurlandG. Plunkett IIIP. RedfordP. Roesch2002Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli.Proc Natl Acad Sci U S A991702017024
- 68. Grozdanov L, Raasch C, Schulze J, Sonnenborn U, Gottschalk G, et al. (2004) Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917. J Bacteriol 186: 5432–5441.L. GrozdanovC. RaaschJ. SchulzeU. SonnenbornG. Gottschalk2004Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917.J Bacteriol18654325441
- 69. Howard SL, Gaunt MW, Hinds J, Witney AA, Stabler R, et al. (2006) Application of comparative phylogenomics to study the evolution of Yersinia enterocolitica and to identify genetic differences relating to pathogenicity. J Bacteriol 188: 3645–3653.SL HowardMW GauntJ. HindsAA WitneyR. Stabler2006Application of comparative phylogenomics to study the evolution of Yersinia enterocolitica and to identify genetic differences relating to pathogenicity.J Bacteriol18836453653
- 70. Kim DW, Lenzen G, Page AL, Legrain P, Sansonetti PJ, et al. (2005) The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes. Proc Natl Acad Sci U S A 102: 14046–14051.DW KimG. LenzenAL PageP. LegrainPJ Sansonetti2005The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes.Proc Natl Acad Sci U S A1021404614051
- 71. Hinchliffe SJ, Isherwood KE, Stabler RA, Prentice MB, Rakin A, et al. (2003) Application of DNA microarrays to study the evolutionary genomics of Yersinia pestis and Yersinia pseudotuberculosis. Genome Res 13: 2018–2029.SJ HinchliffeKE IsherwoodRA StablerMB PrenticeA. Rakin2003Application of DNA microarrays to study the evolutionary genomics of Yersinia pestis and Yersinia pseudotuberculosis.Genome Res1320182029
- 72. Gibbons SJ, Farrugia G (2004) The role of carbon monoxide in the gastrointestinal tract. J Physiol 556: 325–336.SJ GibbonsG. Farrugia2004The role of carbon monoxide in the gastrointestinal tract.J Physiol556325336
- 73. Slee KJ, Skilbeck NW (1992) Epidemiology of Yersinia pseudotuberculosis and Y. enterocolitica infections in sheep in Australia. J Clin Microbiol 30: 712–715.KJ SleeNW Skilbeck1992Epidemiology of Yersinia pseudotuberculosis and Y. enterocolitica infections in sheep in Australia.J Clin Microbiol30712715
- 74. Schaible UE, Kaufmann SH (2005) A nutritive view on the host–pathogen interplay. Trends Microbiol 13: 373–380.UE SchaibleSH Kaufmann2005A nutritive view on the host–pathogen interplay.Trends Microbiol13373380
- 75. Maier RJ, Olson J, Olczak A (2003) Hydrogen-oxidizing capabilities of Helicobacter hepaticus and in vivo availability of the substrate. J Bacteriol 185: 2680–2682.RJ MaierJ. OlsonA. Olczak2003Hydrogen-oxidizing capabilities of Helicobacter hepaticus and in vivo availability of the substrate.J Bacteriol18526802682
- 76. Collyn F, Lety MA, Nair S, Escuyer V, Ben Younes A, et al. (2002) Yersinia pseudotuberculosis harbors a type IV pilus gene cluster that contributes to pathogenicity. Infect Immun 70: 6196–6205.F. CollynMA LetyS. NairV. EscuyerA. Ben Younes2002Yersinia pseudotuberculosis harbors a type IV pilus gene cluster that contributes to pathogenicity.Infect Immun7061966205
- 77. Portnoy DA, Moseley SL, Falkow S (1981) Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun 31: 775–782.DA PortnoySL MoseleyS. Falkow1981Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis.Infect Immun31775782
- 78. Cornelis G, Laroche Y, Balligard G, Sory M-P, Wauters G (1987) Yersinia enterocolitica, a primary model for bacterial invasiveness. Rev Infect Dis 9: 64–87.G. CornelisY. LarocheG. BalligardM-P SoryG. Wauters1987Yersinia enterocolitica, a primary model for bacterial invasiveness.Rev Infect Dis96487
- 79. Darwin AJ, Miller VL (1999) Identification of Yersinia enterocolitica genes affecting survival in an animal host using signature-tagged transposon mutagenesis. Mol Microbiol 32: 51–62.AJ DarwinVL Miller1999Identification of Yersinia enterocolitica genes affecting survival in an animal host using signature-tagged transposon mutagenesis.Mol Microbiol325162
- 80. Young GM, Miller VL (1997) Identification of novel chromosomal loci affecting Yersinia enterocolitica pathogenesis. Mol Microbiol 25: 319–328.GM YoungVL Miller1997Identification of novel chromosomal loci affecting Yersinia enterocolitica pathogenesis.Mol Microbiol25319328
- 81. Parkhill J, Wren BW, Mungall K, Ketley JM, Churcher C, et al. (2000) The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403: 665–668.J. ParkhillBW WrenK. MungallJM KetleyC. Churcher2000The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences.Nature403665668
- 82. Berriman M, Rutherford K (2003) Viewing and annotating sequence data with Artemis. Brief Bioinform 4: 124–132.M. BerrimanK. Rutherford2003Viewing and annotating sequence data with Artemis.Brief Bioinform4124132
- 83. Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG, et al. (2005) ACT: The Artemis Comparison Tool. Bioinformatics 21: 3422–3423.TJ CarverKM RutherfordM. BerrimanMA RajandreamBG Barrell2005ACT: The Artemis Comparison Tool.Bioinformatics2134223423
- 84. Snellings NJ, Popek M, Lindler LE (2001) Complete DNA sequence of Yersinia enterocolitica serotype 0:8 low-calcium-response plasmid reveals a new virulence plasmid-associated replicon. Infect Immun 69: 4627–4638.NJ SnellingsM. PopekLE Lindler2001Complete DNA sequence of Yersinia enterocolitica serotype 0:8 low-calcium-response plasmid reveals a new virulence plasmid-associated replicon.Infect Immun6946274638