Delta-9 desaturases, also known as stearoyl-CoA desaturases, are lipogenic enzymes responsible for the generation of vital components of membranes and energy storage molecules. We have identified a novel nuclear hormone receptor, NHR-80, that regulates delta-9 desaturase gene expression in Caenorhabditis elegans. Here we describe fatty acid compositions, lifespans, and gene expression studies of strains carrying mutations in nhr-80 and in the three genes encoding delta-9 desaturases, fat-5, fat-6, and fat-7. The delta-9 desaturase single mutants display only subtle changes in fatty acid composition and no other visible phenotypes, yet the fat-5;fat-6;fat-7 triple mutant is lethal, revealing that endogenous production of monounsaturated fatty acids is essential for survival. In the absence of FAT-6 or FAT-7, the expression of the remaining desaturases increases, and this ability to compensate depends on NHR-80. We conclude that, like mammals, C. elegans requires adequate synthesis of unsaturated fatty acids and maintains complex regulation of the delta-9 desaturases to achieve optimal fatty acid composition.
The ratio of saturated to unsaturated fatty acids has a profound affect on the fluidity and function of cellular membranes. Animals, plants, and microorganisms regulate the synthesis of unsaturated fatty acids during changing environmental conditions, as well as in response to dietary nutrients. In this paper the authors use a combination of genetic and biochemical approaches to address the regulation of unsaturated fatty acid synthesis in the roundworm Caenorhabditis elegans. They identify a new transcription factor, NHR-80, that activates the expression of genes encoding delta-9 fatty acid desaturases, the enzymes responsible for catalyzing the insertion of double bonds into saturated fatty acid chains. These unsaturated fatty acids are critical components of membranes, as well as fat storage molecules. Experiments presented here demonstrate that the worms require adequate synthesis of unsaturated fatty acids for survival and that they maintain intricate regulation of the three delta-9 desaturase genes in response to different nutrients. Abnormalities in lipid metabolism lead to obesity and diabetes in humans; this study contributes to our understanding of the regulation of this metabolic pathway.
Citation: Brock TJ, Browse J, Watts JL (2006) Genetic Regulation of Unsaturated Fatty Acid Composition in C. elegans. PLoS Genet 2(7): e108. https://doi.org/10.1371/journal.pgen.0020108
Editor: David Valle, Johns Hopkins University School of Medicine, United States of America
Received: March 22, 2006; Accepted: May 30, 2006; Published: July 14, 2006
Copyright: © 2006 Brock et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the National Institutes of Health grant R01-DK074114 and the Agricultural Research Center, Washington State University. Additional funding for TJB was provided by the National Institutes of Health Biotechnology Training Program at WSU.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: GFP, green fluorescent protein; MUFA, monounsaturated fatty acid; NHR, nuclear hormone receptor; PPAR, peroxisome proliferator-activator receptor; QPCR, quantitative RT-PCR; RNAi, RNA interference; SCD, stearoyl-CoA desaturase; SREBP, sterol regulatory element binding protein
Monounsaturated fatty acids (MUFAs) are key components of membrane phospholipids and triglycerides that play important roles in diverse cellular processes such as membrane function, energy storage, and signaling. MUFAs are synthesized from saturated fatty acids by delta-9 (Δ9) desaturases, also known as stearoyl-CoA desaturases (SCDs), which introduce a double bond between the 9th and 10th carbon of a saturated fatty acyl chain. Alterations in the ratio of MUFAs to saturated fatty acids are implicated in heart disease and cancer , the two leading causes of death in the United States . The appropriate ratio between MUFAs and saturated fatty acids is maintained by the activity of the Δ9 desaturases, which are subject to complex regulation . As a key control point in metabolic regulation, Δ9 desaturases could be therapeutic targets for treatment of obesity, diabetes, and cardiovascular disease.
The Δ9 desaturases are ubiquitous enzymes in eukaryotes, found in organisms from yeast to humans. Yeast have one Δ9 desaturase, Ole1p, and mutants that lack this activity are not able to survive without exogenous supplementation of unsaturated fatty acids . Mice have four Δ9 desaturases, each having a unique expression pattern [5,6]. Mutant analysis has revealed distinct roles for SCD1 and SCD2. SCD1 is important for adult energy metabolism and lipid synthesis , while SCD2 is involved in lipid synthesis during embryonic development . In humans, two SCD isoforms, hSCD1 and hSCD5, have been described [9,10]. A variety of environmental and physiological signals affect the expression of Δ9 desaturases. Diets rich in unsaturated fatty acids decrease Δ9 desaturase expression, while high carbohydrate consumption increases expression . Decreased temperature leads to increases in Δ9 desaturase gene expression in poikilotherms . In addition, endogenous hormones such as leptin and glucagon cause a decrease in Δ9 desaturase gene expression, while insulin has the opposite effect .
Sterol regulatory element binding proteins (SREBPs) and peroxisome proliferator-activator receptor protein-alpha (PPARα) have been identified as key transcriptional regulators of SCD1 gene expression in mammals . The SREBP-1 gene encodes a transcription factor that stimulates expression of genes involved in fatty acid biosynthesis, including SCD1 , while the SREBP-2 gene product stimulates genes involved in cholesterol biosynthesis . PPARα is one of a family of nuclear hormone receptors (NHRs), that, upon ligand binding, acts as a heterodimer with the retinoid X receptor to induce transcription of target fat metabolism genes . PPARα, like all NHRs, contains a hydrophobic pocket for ligand binding and a DNA binding domain for interacting with the promoters of target genes. The targets of PPARα include genes for the β-oxidation enzymes, SCDs, and other fatty acid desaturases [15,16]. The other members of the PPAR family, PPARδ and PPARγ are also involved in regulation of fat metabolism . These regulators have unique roles due to differences in their gene expression patterns and regulatory activities.
Caenorhabditis elegans is becoming recognized as an important model for the study of fat metabolism. These animals synthesize a wide variety of fatty acids using a Δ12 desaturase, an Δ3 desaturase, a Δ5 desaturase, a Δ6 desaturase, and three Δ9 desaturases [18,19]. C. elegans can also incorporate dietary fatty acids into lipids, allowing researchers to modify the fatty acid composition of live animals [20,21]. In an RNAi (RNA interference) screen, genes were identified that altered fat storage and many of these genes have mammalian counterparts known to function in fat metabolism . In addition, mutant analysis offers insight into pathways known to regulate fat storage in both nematodes and mammals such as the insulin-signaling pathway . A recent study established a role for NHR-49, as a regulator of lipid homeostasis . The nhr-49 mutants have increased levels of the saturated fatty acid 18:0, higher fat accumulation, and a shorter lifespan than wild-type animals. NHR-49 is also required for inducing Δ9 desaturase expression in well-fed animals .
To gain a deeper understanding of fatty acid metabolism in C. elegans we have characterized the three Δ9 desaturase mutants using biochemistry, gene expression, and phenotypic analysis. While the three Δ9 desaturase single mutants, fat-5, fat-6, and fat-7 display few differences from wild type, we show that they compensate for loss of one isoform by regulated induction of the remaining Δ9 desaturase genes. This induction depends on NHR-80, a novel NHR that we have identified as a regulator of desaturase expression. Furthermore, the fat-5;fat-6;fat-7 triple mutant is unable to survive, revealing that endogenous production of monounsaturated fatty acids is essential for survival under standard growth conditions. The Δ9 desaturase genes and their transcriptional regulators are vital for maintaining optimal fatty acid unsaturation and proper membrane composition.
Identification of NHR-80 as a Regulator of Fatty Acid Metabolism
In our search to identify the desaturases and elongases involved in generation of unsaturated fatty acids in C. elegans, we performed a genetic screen to identify mutants with altered fatty acid profiles . In the process of identifying the molecular nature of one mutation, we used RNAi against 156 genes at the end of Chromosome III to determine the fatty acid composition of animals when each of these genes was inactivated. We found an RNAi clone, nhr-80, that caused C. elegans to accumulate increased levels of 18:0. NHR-80 is a member of the NHR family of transcription factors in C. elegans . To further examine this gene we obtained a deletion allele from the National BioResource Program for the Experimental Animal C. elegans, Japan. The nhr-80(tm1011) mutant carries a 446-bp deletion that eliminates approximately half of the nucleotides in the second exon and all of the third exon (Figure 1). Like the nhr-80(RNAi) worms, these mutants also showed an accumulation of 18:0 and reduction of 18:1 Δ9 (Figure 2) indicating that nhr-80(tm1011) is likely to be a loss of function mutation. In the nhr-80 mutants, 18:0 accounts for about 10.2 ± 0.3% of the total fatty acids and 18:1 Δ9 accounts for 2.2 ± 0.1%, as compared with 6.8 ± 0.2% and 3.2 ± 0.1%, respectively, in the wild type. The difference between these fatty acids in the nhr-80 mutants and wild-type animals is significant, with p < 0.01 for both fatty acids. The changes in fatty acid composition shown for the nhr-80 mutants in Figure 2 are similar to those reported for the nhr-49 mutants. In those mutants the ratio of 18:0 to 18:1 Δ9 was 4.3 compared to a ratio of 1.9 in wild-type animals . In our analysis of the nhr-80 mutants the 18:0 to 18:1 Δ9 ratio was 4.6 compared to the wild-type ratio of 2.2. The nhr-80 mutants are viable and fertile indicating this change in fatty acid composition, though significant, does not affect essential functions of the animal.
(A) nhr-80 is composed of a zinc finger domain (green boxes) and a ligand-binding domain (light blue boxes). nhr-80(tm1011) contains a 446-bp deletion (light grey bar).
(B) fat-5, fat-6, and fat-7 all contain four trans-membrane domains (dark blue boxes) and three histidine boxes (red boxes). fat-5(tm420) consists of a 779-bp deletion (light grey bar). fat-6(tm331) contains a 1,232-bp deletion (light grey bar), and a 428-bp insertion (purple bar). The fat-7 alleles are point mutations with fat-7(wa36), creating a premature stop codon and fat-7(wa37) changing a conserved histidine into a tyrosine.
Relative abundance of selected fatty acid species expressed as percentage of total fatty acid as determined by gas chromatography analysis. The nhr-80 mutants have significantly higher levels of 18:0 and lower levels of 16:0 and 18:1 Δ9 than wild type. Error bars represent the standard error. *significant differences between wild type and nhr-80 mutant, p < 0.01.
Although two NHR mutant lines, nhr-49 and nhr-80, show increased 18:0 as compared with wild-type worms, not all NHR mutants cause these changes in fat metabolism . Both of these transcription factors are proposed to be derived from the same ancestral gene that also is the progenitor of the mammalian gene encoding hepatocyte nuclear factor 4 , which in mammals, binds to fatty acids as ligands and is a key activator of lipid and cholesterol metabolism genes .
In addition to the change in fatty acid composition, the nhr-49 mutants display an increase in fat storage based on staining of whole worms with the lipophilic dye Nile red . In the nhr-80 mutants, we observed no increase in Nile red staining as compared to wild type (unpublished data) indicating no increase in fat storage. To confirm this, we tested fat storage in the nhr-80 mutants by measuring the percent triglycerides in the total lipids. In the nhr-80 mutants triglycerides comprised 44 ± 1% of the total lipids as compared to 45 ± 1% in wild type signifying no increase in fat storage. Thus the increased 18:0 accumulation and increased 18:0 to 18:1 Δ9 ratio does not cause increased triglyceride synthesis. However, the altered fatty acid profile of nhr-80 mutants indicates a role for NHR-80 in the regulation of fatty acid metabolism in C. elegans.
NHR-80 Is Required for Normal Expression of Δ9 Desaturases
As NHR-80 is a transcription factor expressed in the intestine , the major site of fat metabolism in C. elegans, the increased 18:0 accumulation in the nhr-80 mutants may be due to a reduced expression of the Δ9 desaturase genes. To test this we used quantitative RT-PCR (QPCR) to measure gene expression with primers designed to amplify fat-5, fat-6, and fat-7, along with the control genes tbb-2 (β-tubulin) and ubc-2 (ubiquitin-conjugating enzyme, E2). Relative expression of these genes was examined in wild-type and nhr-80 mutant adult populations and we found that expression of all three Δ9 desaturases was decreased in the nhr-80 mutants relative to wild type for eight experimental replicates (Figure 3). On average, fat-5 and fat-6 expression were reduced by 66% and 22% respectively, while fat-7 expression was almost completely eliminated in the nhr-80 mutants.
(A) Gene expression by QPCR in the nhr-80 mutant reveals a decrease in expression of the Δ9 desaturase genes relative to wild type. Error bars represent standard error.
(B) Transformed lines expressing Δ9 desaturase gene GFP fusions grown to adulthood on empty vector control bacteria or nhr-80(RNAi) bacteria. Exposure times for photographs were adjusted due to different GFP expression in the three genes, although the exposure time for the two treatments was kept the same for each genotype. The exposure time for the fat-5::GFP worms was 1/4 s, for the fat-6::GFP worms was 1/30 s, and for the fat-7::GFP worms was 1/8 s. After 4 d, there is a dramatic reduction in Δ9 desaturase gene expression in the intestine for fat-5::GFP and fat-7::GFP lines grown on nhr-80(RNAi).
To determine if the expression pattern of nhr-80 overlapped with the expression pattern of the Δ9 desaturases we created two green fluorescent protein (GFP)-fusion expressing lines for each of the Δ9 desaturase genes. Like nhr-80, all three Δ9 desaturase genes were expressed in the intestine in adult worms (Figure 3B), and in all four larval stages (unpublished data). The fat-5 promoter::GFP expressing lines showed additional expression in the pharynx and tail cells after hatching and throughout the lifespan. The fat-6 whole gene::GFP expressing lines displayed additional expression in the hypodermis in all life stages. The overlapping intestinal expression for all three Δ9 desaturase genes indicates possible functional redundancy. The potential role for fat-5 in the pharynx and fat-6 in the hypodermis remain to be determined; however, the constitutive expression of these genes in the intestine is consistent with a central role for Δ9 desaturation in normal C. elegans function.
To confirm the regulation of the Δ9 desaturases by NHR-80, lines expressing the GFP fusions were grown on nhr-80(RNAi) bacteria. Transformed adults were allowed to lay eggs on nhr-80(RNAi) and control bacteria. The adults were removed and about 20 of the progeny were examined for GFP expression after 4 d of growth. Representative samples are shown in Figure 3B. Expression of fat-7 whole gene::GFP was completely eliminated by the RNAi treatment. Expression of fat-5 promoter::GFP was decreased but only in the intestine, not in the pharynx. Expression of fat-6 whole gene::GFP was also slightly decreased. The reduction of fat-5 and fat-6 expression and the elimination of fat-7 expression likely accounts for the changes in fatty acid composition observed in the nhr-80 mutant. Similar to the nhr-80 mutants, the nhr-49 mutants exhibited an increased level of 18:0 accumulation and a decrease in expression of the Δ9 fatty acid desaturase genes by QPCR with fat-5 and fat-7 as the most reduced . However, the nhr-49 mutants have increased fat storage, which is not seen in the nhr-80 mutants, and show decreased expression of two genes that encode proteins that participate in the mitochondrial β-oxidation pathway, an enoyl-CoA hydratase gene (C29F3.1, ech-1) and an acyl-CoA synthetase gene (F28F8.2, acs-2). We tested the expression of ech-1 and acs-2 in the nhr-80 mutants by QPCR and found that there was no change in expression levels relative to wild-type expression. This is consistent with the normal level of fat storage seen in the nhr-80 mutants. Though both NHR-49 and NHR-80 are required for Δ9 desaturase expression, their effects on fatty acid metabolism in C. elegans are not identical; NHR-49 appears to regulate a wider range of lipid homeostasis pathways.
nhr-80 Mutants Do Not Die Early like nhr-49 Mutants
It has been suggested that shifts in the ratio of saturated fatty acids to MUFAs in C. elegans may lead to a decreased lifespan. For example, the change in the ratio of 18:0 to 18:1 Δ9 from 1.9 in wild type to 4.3 in nhr-49 mutants has been proposed to cause a substantial reduction in lifespan from 15–18 d in wild type, to 6–8 d in nhr-49 mutants . We examined the lifespan of the nhr-80 mutants (Figure 4) and found that they may have slightly shorter lifespans than wild type but live considerably longer than nhr-49 mutants despite having a similar fatty acid composition. In this experiment, the average lifespan of the nhr-80 mutant was 12.5 ± 0.5 d as compared to 13.9 ± 0.4 d in wild-type animals and 8.2 ± 0.2 d in nhr-49 mutants when grown at 25 °C. These data indicate a 10% decrease in mean lifespan between wild type and nhr-80 mutants, the difference between wild type and nhr-49 mutants is much greater with a 41% reduction in mean lifespan. The early death of the nhr-49 does not seem to be caused solely by an elimination of fat-7 expression or an increase in the ratio of 18:0 to 18:1 Δ9 since nhr-80 mutants also show these characteristics but do not have a dramatically shortened lifespan. It is possible that the shorter lifespan of the nhr-49 mutants is caused by metabolic changes due to other targets of NHR-49 regulation.
(A) Life span of nhr-49, nhr-80, and wild type at 25 °C. The nhr-80 and wild type display a considerably longer life than the nhr-49 mutants. All lifespan data are presented as mean lifespan ± standard error (total number of animals scored). Wild type: 13.9 ± 0.4 (83); nhr-49: 8.2 ± 0.2 (80); nhr-80: 12.5 ± 0.5 (70).
(B) Life span of fat-5, fat-6, fat-7, and wild type at 25 °C. The fat-5, fat-6, and fat-7 mutants exhibit a lifespan similar to wild type. Wild type: 13.9 ± 0.4 (83); fat-5: 15.9 ± 0.6 (82); fat-6: 14.2 ± 0.5 (82); fat-7: 15.0 ± 0.5 (75).
C. elegans Δ9 Desaturases Are Redundant under Standard Growth Conditions
Previous studies revealed that the three C. elegans Δ9 desaturase isozymes display different substrate specificities. While FAT-6 and FAT-7 preferentially desaturate stearic acid (18:0), similar to most of the characterized SCDs, FAT-5 prefers palmitic acid (16:0) and has little or no activity on stearic acid . We obtained Δ9 desaturase single mutants to further characterize the roles of these three desaturases. We obtained fat-5(tm420) and fat-6(tm331) deletion alleles from the National BioResource Program for the Experimental Animal C. elegans, Japan (Figure 1B). The fat-5 allele has a 779-bp deletion early in the coding sequence that eliminates two of the conserved histidine boxes and two of the trans-membrane domains. The fat-6 allele has a 1,232-bp deletion and a 428-bp insertion. The deletion is early in the coding sequence and also eliminates two of the conserved histidine-rich regions and two trans-membrane domains. Both of these mutations are likely null. The fat-7(tm326) deletion allele is available but molecular analysis of this allele led us to believe that a more extensive genetic disruption had occurred that affects other genes in addition to fat-7. Alternative fat-7 alleles were isolated by TILLING (Targeting Induced Local Lesions IN Genomes)  and are single base pair changes (Figure 1B). The fat-7(wa36) allele is a C to T mutation that leads to a premature stop codon that eliminates two trans-membrane domains and one of the conserved histidine boxes required for activity of the rat SCD enzyme , indicating that this allele is, at a minimum, a strong reduction-of-function allele. The fat-7(wa37) allele is a C to T mutation that replaces a conserved histidine with tyrosine . Because these histidines are expected to be required for Δ9 desaturase activity we expressed this allele in mutant yeast that lack Δ9 desaturase activity (ole1 mutants). The mutant fat-7(wa37) did not support growth of the ole1 mutant yeast, whereas expression of wild-type fat-7 did allow growth . Phenotypic characterization including fatty acid composition and lifespan with fat-7(wa37) showed no difference from fat-7(wa36) therefore only data from fat-7(wa36) are reported here.
The C. elegans Δ9 desaturase mutants show subtle differences from wild type in their fatty acid profile when grown on an Escherichia coli lawn on NGM plates at 20 °C (Figure 5). Compared to wild type (4.1 ± 0.2%), the fat-5 mutants display decreased 16:1 Δ9 (3.4 ± 0.1%), which is the product of FAT-5 desaturation based on the substrate specificity exhibited in yeast . The fat-6 mutants exhibit a significant increase in their accumulation of the predicted substrate of FAT-6, 18:0 (9.6 ± 0.2%), over wild type (7.0 ± 0.2%).
(A−C) There is little change in fatty acid composition for fat-5 (A), fat-6 (B), and fat-7 (C) mutants compared to wild-type worms when grown under standard growth conditions with OP50 E. coli as the sole food source.
(D−F) Axenic growth conditions for wild-type worms and fat-5 (D), fat-6 (E), and fat-7 (F) mutants reveal major changes in fatty acid composition for fat-5 and fat-6 mutants compared to wild-type worms. In all graphs relative abundance of selected fatty acid species is expressed as percentage of total fatty acid as determined by gas chromatography analysis. Error bars represent the standard error. *significant difference from wild type, p < 0.01
The Δ9 desaturase mutants are indistinguishable from wild type in other characteristics tested including growth rate, reproduction, and behavior. The lack of phenotype indicates that subtle changes in fatty acid composition have no apparent effect and that the desaturases are functionally redundant. To determine if gene expression changes are involved in compensating for the lack of one isozyme, we examined expression of the Δ9 desaturase genes in the fat-5, fat-6, and fat-7 mutants (Figure 6). In the fat-6 mutants, fat-7 expression is increased approximately 4-fold over wild type and fat-5 expression is increased 2–3-fold over wild type. In the fat-7 mutant, expression of fat-6 and fat-5 is also slightly increased over wild type. The fat-5 mutant shows little difference from wild type in fat-6 and fat-7 expression.
Gene expression by QPCR in the fat-5, fat-6, and fat-7 mutants relative to wild type reveals an increase in Δ9 desaturase gene expression in the fat-6 and fat-7 mutants, relative to wild type. Error bars represent standard error of 7–12 experiments.
Axenic Growth Reveals Substrate Specificity of the Δ9 Desaturases
The standard strain of E. coli on which C. elegans are maintained in the laboratory contains palmitic (16:0), palmitoleic (16:1 Δ9), and vaccenic (18:1 Δ11), but not oleic acid (18:1 Δ9) or polyunsaturated fatty acids . When worms eat these bacteria they incorporate the fatty acids in their lipids. To test the fatty acid composition of the Δ9 desaturase mutants grown on a different food source we grew the C. elegans strains in axenic media devoid of bacteria. This liquid media provides amino acids, vitamins, growth factors, and heme . Our measurements reveal that the axenic media contains palmitic, palmitoleic, oleic and linoleic acids, but no vaccenic acid (unpublished data). Wild-type worms grow considerably more slowly under the axenic growth conditions, and the fatty acid profile is also dramatically different. In axenic culture, wild-type worms accumulate higher levels of 16:0, 18:0, and 18:1 Δ9, while they produce lesser amounts of 20:5 (Figure 5A and 5D).
The Δ9 desaturase mutants show greater differences in fatty acid composition when grown axenically than when grown on E. coli plates (Figure 5D–5F). Comparing the fatty acid composition of the fat-5 mutant with wild type we observe an increase in 16:0 (19 ± 1% versus 12 ± 1%) and a decrease in 16:1 Δ9 (1.1 ± 0.4% versus 3.0 ± 0.3%) and 18:1 Δ11 (3.8 ± −0.1% versus 17 ± 1%) in the fat-5 mutants. The fat-6 mutants also display dramatic differences from wild type, with an increase in 18:0 (16.7 ± 0.8% versus 10.9 ± 0.7%) and a decrease in 18:1 Δ9 (11.3 ± 0.6% versus 21.8 ± 0.5%) in the fat-6 mutants. The fatty acid composition of fat-7 mutants does not differ significantly from wild type, indicating that fat-6 can completely compensate for fat-7 in axenic culture and therefore that FAT-7 does not play an important role in maintaining proper fatty acid composition under axenic conditions. The dramatic reduction of 16:1 Δ9 and 18:1 Δ11 fatty acids in fat-5 mutants and 18:1Δ9 in fat-6 mutants grown in axenic culture is the first evidence that these enzymes have the same substrate specificity in C. elegans as they do when expressed in yeast .
To determine whether the levels of Δ9 desaturase gene expression are modulated in response to diet we examined the expression of fat-5, fat-6, and fat-7 genes in axenic media and on E. coli seeded plates using QPCR. We found that compared to worms grown on E. coli, fat-5 expression increases about 6-fold in axenic media. In contrast, fat-6 expression is maintained at similar levels while fat-7 expression is dramatically decreased in axenic media (Figure S1).
Single Δ9 Desaturase Mutants Have No Early-Death Phenotype
Previous studies investigating the C. elegans Δ9 desaturases have used RNAi to deplete fat-7 expression and have suggested that fat-7 expression is required to maintain a normal lifespan [23,24]. Based on these results, it was proposed that the reduced expression of fat-7 was the cause of the short lifespan in the nhr-49 mutants . However, the fat-7mutants used in our experiment as well as the other Δ9 desaturase mutants, fat-5 and fat-6, do not exhibit an early death phenotype (Figure 4B). The average lifespan of the fat-5 mutants is 15.8 ± 0.6 d, the fat-6 mutant is 14.2 ± 0.5 d, and the fat-7 mutant is 15.0 ± 0.5 d, as compared with a lifespan of 13.9 ± 0.4 d in wild-type animals. In this experiment, the fat-5 mutant displayed a slight but significant (p < 0.01) increase in lifespan over wild type, while the fat-6 and fat-7 mutants were not significantly different from wild type in average lifespan.
Our experiments with the fat-7 mutant do not support the requirement for fat-7 for normal lifespan as proposed from studies using fat-7(RNAi) [23,24]. Additionally, fat-7(RNAi) revealed major changes in fatty acid composition and a reduction of fat storage  that was not observed in the fat-7 mutants. The RNAi phenotype observed could be due to transitive secondary RNAi effect  as fat-7 has 84% nucleotide identity with fat-6 including eight regions of 21–44 nucleotides with 100% identity. Van Gilst et al. report that fat-7(RNAi) did not reduce fat-6 expression when measured by QPCR ; however, we observe an elimination of fat-6 expression when fat-6 whole gene::GFP lines were grown on fat-7(RNAi) (unpublished data). In addition, it is possible that compensation by the third Δ9 desaturase, fat-5, is inhibited in the fat-7(RNAi). Because the fat-7 loss-of-function mutant is wild type for fatty acid composition and lifespan it must be concluded that fat-7(RNAi) is having off-target effects on the worm.
Δ9 Desaturase Activity and Monounsaturated Fatty Acids Are Required for Survival
Because the Δ9 desaturase genes appear to compensate for each other, we constructed a fat-5;fat-6;fat-7 triple mutant lacking all three Δ9 desaturases. We expected these mutants would be unable to survive under standard growth conditions, so we supplemented the worms with a combination of 18:1 Δ9, 18:2 ω6, and 20:5 ω3 dietary fatty acids. After identifying the fat-5;fat-6;fat-7 triple mutant, we moved the worms to plates without fatty acid supplementation and found that indeed these worms could not survive. Larvae that hatch from eggs laid on unsupplemented plates arrest in the L1 stage, while L3 and L4 stage larvae that are moved from supplemented to unsupplemented plates develop into thin, sterile adults with reduced movement and early death. The MUFAs provided by the standard E. coli diet are not sufficient for survival in the fat-5;fat-6;fat-7 triple mutant. Thus C. elegans have a requirement for a certain level of Δ9 desaturation that cannot be met by the standard E. coli diet. The yeast Δ9 desaturase mutant, ole1, is also unable to grow without supplementation . The fat-5;fat-6;fat-7 triple mutant is the first multicellular organism generated that lacks all endogenous Δ9 desaturase activity.
To examine genetic interaction between nhr-80 and fat-6, the most highly expressed Δ9 desaturase, we constructed the fat-6;nhr-80 double mutant using plates supplemented with dietary fatty acids. When we removed the fat-6;nhr-80 double mutants to unsupplemented plates we found that these worms also did not survive. Since the nhr-80(RNAi) phenotype resembles the nhr-80 mutants, we used RNAi in combination with the Δ9 desaturase mutants to study this interaction further. The fat-6 mutants, when grown on nhr-80(RNAi) from eggs, become thin, slow growing, and reproductively inviable after 4 d of growth (Figure 7). They also accumulate very high levels of 18:0 (Figure 7B). The fat-6 mutants grown on nhr-80(RNAi) accumulate 34 ± 1% of their fatty acids as 18:0 as compared to 9.1 ± 0.1% when fat-6 is grown on control bacteria or 14 ± 2% when wild-type worms are grown on nhr-80(RNAi) bacteria. Although 18:0 also accumulates in the fat-5 and fat-7 mutants grown on nhr-80(RNAi), the extent of 18:0 accumulation is not as dramatic as observed in fat-6 (Figure 7B) and they do not show a synthetic lethality (Figure 7A).
(A) Photographs showing adult worms after 4 d of growth on nhr-80(RNAi) and empty vector control bacteria. The fat-6 mutants grown on nhr-80(RNAi) are thin, pale, and produce no viable progeny.
(B) Relative abundance of 18:0 expressed as a percentage of total fatty acid as determined by gas chromatography analysis. The fat-6 mutants grown on nhr-80(RNAi) (n = 5) accumulate much higher levels than fat-6 mutants grown on control (n = 7) and wild type grown on nhr-80(RNAi) (n = 6). *significant differences from growth on control bacteria, p < 0.01.
(C) Effects of nhr-80 on Δ9 desaturase gene expression in fat-5, fat-6, and fat-7 mutants. QPCR in fat-5, fat-6, fat-7, and wild type for worms grown on empty vector control bacteria (ev) and nhr-80(RNAi) (80i) (n = 6). Values are expressed relative to fat-6 expression in wild-type worms grown on control bacteria. For all graphs error bars represent standard error.
One explanation for the synthetic lethality of fat-6;nhr-80 double mutants is that NHR-80 is required for the increased fat-5 and fat-7 expression in the fat-6 mutant. To test this we examined the expression of the Δ9 desaturase genes in the fat-5, fat-6, and fat-7 mutants grown on nhr-80(RNAi). We found that expression of fat-7 in the fat-6;nhr-80(RNAi) is less than 10% of the expression of fat-7 in the fat-6 mutants grown on control bacteria, consistent with the notion that NHR-80 is required to induce the expression of fat-7 (Figure 7C). We graphed the relative expression values, setting fat-6 expression in wild-type worms grown on control bacteria as 100%. In wild-type worms on control bacteria fat-6 is the most highly expressed Δ9 desaturase gene and fat-5 and fat-7 are expressed at 3.6 ± 0.2% and 6.5 ± 0.6% of the level of fat-6 respectively. When the wild-type worms are grown on nhr-80(RNAi) we observe a similar relative decrease in Δ9 desaturase gene expression seen in the nhr-80 mutants (Figure 3A). Comparing the fat-5 and fat-7 mutants grown on control with those grown on nhr-80(RNAi) reveals a decrease in Δ9 desaturase gene expression. However, the biggest difference is seen in the fat-6 mutants. When these animals are grown on control bacteria fat-7 is increased in expression 37-fold over wild type. When fat-6 is grown on nhr-80(RNAi) the fat-7 relative expression is only a 3-fold increase over wild type.
The overall amount of Δ9 desaturase gene expression is approximately equal for all worms grown on nhr-80(RNAi), but only fat-6 displays synthetic lethality with nhr-80. This could be due to the composition of the Δ9 desaturase gene expression. When wild type, fat-5, or fat-7 are grown on nhr-80(RNAi) fat-6 is the major gene expressed suggesting its central importance for Δ9 desaturation activity. The fat-6 mutants lack fat-6 expression and compensate by substantially increasing fat-7 expression when grown on control bacteria. It is noteworthy that under these conditions fat-7 expression is increased 37-fold, perhaps indicating that fat-7 is not as effective at Δ9 desaturation as fat-6 due to differences in tissue specific expression, translation efficiency or protein stability. When the fat-6 mutants are grown on nhr-80(RNAi) they are unable to compensate with an increase in fat-7 expression to an appropriate level and this may cause their reduced survival. Thus NHR-80 is required for increasing fat-7 expression in situations where higher fat-7 levels are necessary and consequently defines a critical regulator of fatty acid metabolism.
Our characterization of the novel NHR-80 and the family of C. elegans Δ9 desaturase mutants enhances our understanding of the regulation of lipid homeostasis. Maintaining appropriate fatty acid composition is essential and without sufficient Δ9 desaturase activity both the fat-5;fat-6;fat-7 triple mutants and the fat-6;nhr-80 double mutants are unable to survive. The integration of endogenous and environmental signals by NHRs such as NHR-80 precisely regulates the expression of the Δ9 desaturase genes and the production of monounsaturated fatty acids leads to optimal membrane fluidity and fat storage.
Materials and Methods
Culture of nematodes.
Unless otherwise noted, C. elegans were grown on nematode growth media (NGM) plates with OP50 strain of E. coli as a food source . The wild-type strain used is strain N2. Mutant strains obtained from Shohei Mitani and Edwin Cuppen were outcrossed at least four times to the N2 strain. The nhr-80(RNAi) construct, as well as the others used in the screen of Chromosome III, are from the Ahringer RNAi library  and were used as described . As a control for RNAi experiments, nematodes were grown on NGM plates with the HT115 strain of E. coli transformed with pPD129.36 (L4440) empty vector plasmid. The axenic culture media consisted of 3% soy peptone, 3% yeast extract, 0.5 mg/ml hemoglobin in 1M KOH, and 20% ultra-high temperature pasteurized skim milk . Worms were grown in this liquid culture at room temperature (22–23 °C) with constant shaking. To make plates supplemented with dietary fatty acids a 0.1 M stock solution of fatty acid sodium salts (NuCheck Prep, Elysian, Minnesota, United States) in water was prepared fresh for each supplementation experiment. The fatty acid stock was added slowly to NGM containing 0.1% tergitol. Plates were poured, covered and allowed to dry in the dark at room temperature overnight. The OP50 strain of E. coli was added to each plate and allowed to dry for at least one night .
Fatty acid and lipid analysis.
For fatty acid analysis, adult nematodes were washed from plates and allowed to settle. The excess water was removed from the worm pellet and 1 ml of 2.5% methanolic H2SO4 was added and incubated at 80 °C for 1 h to generate fatty acid methyl esters, which were extracted by adding 1.5 ml water and 0.2 ml hexane. The hexane was sampled for determination of fatty acid composition by gas chromatography on an SP-2380 fused silica capillary column (Supelco, Bellefonte, Pennsylvania, United States) using an Agilent (Palo Alto, California, United States) 6890 series gas chromatograph .
For lipid analysis, about 0.5 ml of adult nematodes were collected in a glass tube and frozen. Lipids were extracted by incubation in (1:1) chloroform/methanol overnight at −20 °C. The samples were washed with 2.2 ml Hajra's solution (0.2M H3PO4, 1M KCl) and the chloroform phase containing the lipids was isolated. The silica gel HL plates (Analtech, Newark, Delaware, United States) were activated by incubation at 110 °C for 1 h and 15 min. The samples were loaded onto the thin layer chromatography plates along with lipid standards (Sigma, St. Louis, Missouri, United States). The plates were run with a 65:43:3:2.5 chloroform/methanol/water/acetic acid solvent mixture until the solvent front was three-fourths of the way up the plate. The plate was dried, a new solvent mixture of 80:20:2 hexane/diethyl ether/acetic acid was added, and the plate was run until the solvent front reached the top of the plate. The marker lanes were visualized using iodine vapor and the corresponding bands for triglycerides and individual phospholipids in the silica gel were scraped into individual tubes. To quantitate, 50 μg of 15:0 free fatty acid was added to each tube as an internal standard and fatty acid analysis was performed by gas chromatography as described above .
Adult nematodes were harvested and frozen in liquid nitrogen. RNA was prepared using TRIzol Reagent (Invitrogen, Carlsbad, California, United States). A DNA-FREE RNA kit (Zymo Research, Orange, California, United States) was used for Dnase treatment and purification. After quantification, 1 μg of RNA was used in a reverse-transcription reaction with SuperScriptIII (Invitrogen) to generate cDNA. Primer sequences for the Δ9 desaturase genes and the reference genes were designed using PrimerQuest software at http://www.idtdna.com. Other primer sequences were obtained from Dr. Marc Van Gilst . Primer sequences are listed in Table S1. The PCR mixture consisted of 0.3 μM primers, cDNA, ROX, and 1× SYBR green mix (Invitrogen Platinum SYBR green qPCR Supermix UDG). The QPCR was run and monitored on a MX3000P (Stratagene, La Jolla, California, United States). Relative abundance was determined using the ΔΔCt method and an average of the expression of the reference genes tbb-2 and ubc-2 to control for template levels .
Construction of GFP fusions and microinjection.
Fusion PCR was used to create translational fat-5, fat-6, and fat-7 GFP constructs. The promoters and coding sequences of fat-6 and fat-7 and the promoter and first exon of fat-5 were amplified from genomic DNA. The upstream regulatory region for fat-5 was 4 kb, for fat-6 was 2.6 kb, and for fat-7 was 3.0 kb. GFP was amplified from the Fire vector pPD95.75 including the entire coding sequence and a termination sequence. These PCR products were fused together in a final PCR using nested primers . These fusions were microinjected into lin-15 mutant C. elegans along with a rescuing plasmid, pJM23, containing the wild-type lin-15 gene [39,40]. Multiple independent lines of nematodes without the lin-15 phenotype were selected and examined for GFP expression using fluorescence microscopy on an Olympus IX70 microscope.
Aging experiments were performed on adult nematodes grown at 25 °C. Worms were moved to plates containing 5-fluoro-2′-deoxyuridine (Sigma) at the fourth larval stage of development (L4). Live animals were assayed for movement in response to touch every 1–2 d .
Generation of fat-5;fat-6;fat-7 triple mutants and fat-6;nhr-80 double mutants.
The fat-6(tm331);fat-7(wa36) hermaphrodites were crossed with fat-5(tm420);fat-7(wa36) males on plates supplemented with 18:1 Δ9. The F1 generation was moved to new 18:1 Δ9 supplemented plates and their progeny were moved to plates supplemented with a combination of 18:1 Δ9, 18:2 ω6, and 20:5 ω3. After the F2 generation reproduced, the adults were harvested for single worm PCR to determine the genotype . The fat-5 and fat-6 mutations were monitored using the difference in amplicon size between wild-type and mutant alleles due to the large deletions. The wild-type products were 1,100 bp for fat-5 and 1,457 bp, for fat-6 compared with the mutant products of 321 bp and 652 bp, respectively. All cross-progeny were homozygous for the fat-7 single base pair mutation.
To generate nhr-80;fat-6 double mutants we crossed fat-6 males with nhr-80 hermaphrodites on 18:1 Δ9 supplemented plates and isolated the F1 generation onto new supplemented plates. The F2s were moved to fresh 18:1 Δ9 supplemented plates and allowed to reproduce then single worm PCR was used to identify nhr-80;fat-6 double mutants. The nhr-80 wild-type allele generated a PCR product of 745 bp, whereas the nhr-80(tm1011) mutant allele generated a product 298 bp in length.
Figure S1. Expression of Δ9 Desaturase Genes in Wild-Type Worms Grown on E. coli (OP50) Seeded Plates or Axenic Liquid Media
The percent expression shown is relative to fat-6 expression on E. coli plates, which is set at 100%. In wild-type worms grown in axenic culture the expression of fat-5 is increased and the fat-7 expression is nearly eliminated relative to expression in wild-type worms grown on E. coli (OP50) plates. Relative to fat-6 expression, fat-5 and fat-7 expression is higher in wild-type worms grown on E. coli (OP50) compared to wild-type worms grown on E. coli (HT115) (Figure 7C). Error bars are SEM, n = 3 replicates for plate grown and n = 6 replicates for axenic cultured nematodes.
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Table S1. Sequence of DNA Primers Used in These Studies
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The GenBank (http://www.ncbi.nlm.nih.gov/Genbank) accession numbers for genes used in this study are nhr-80 (H10E21.3) (AY204179), fat-5 (W06D12.3) (AF260242), fat-6 (VZK822L.1) (AF260244), and fat-7 (F10D2.9) (AF260243).
We thank Dr. Shohei Mitani (Tokyo Women's Medical University, Japan) and the National BioResource Project for the Nematode (Japan) for providing the nhr-80(tm1011), fat-5(tm420), fat-6(tm331), and fat-7(tm326) mutants. We are also grateful to Dr. Edwin Cuppen (Hubrecht Laboratory, The Netherlands) who screened his TILLING library for the fat-7(wa36 and wa37) mutants. We thank Dr. Andy Fire (Stanford University, California) for vectors and Dr. Marc Van Gilst (Fred Hutchinson Cancer Research Center, Washington) for the primer sequences for the metabolism genes used in QPCR and for comments on the manuscript.
TJB, JB, and JLW conceived and designed the experiments. TB performed the experiments and analyzed the data. TJB, JB, and JLW wrote the paper.
- 1. Ntambi JM, Miyazaki M, Dobrzyn A (2004) Regulation of stearoyl-CoA desaturase expression. Lipids 39: 1061–1065.JM NtambiM. MiyazakiA. Dobrzyn2004Regulation of stearoyl-CoA desaturase expression.Lipids3910611065
- 2. Anderson RN, Smith BL (2005) Deaths: Leading Causes for 2002. National Vital Statistics Report 53: 1–89.RN AndersonBL Smith2005Deaths: Leading Causes for 2002.National Vital Statistics Report53189
- 3. Ntambi JM, Miyazaki M (2004) Regulation of stearoyl-CoA desaturases and role in metabolism. Prog Lipid Res 43: 91–104.JM NtambiM. Miyazaki2004Regulation of stearoyl-CoA desaturases and role in metabolism.Prog Lipid Res4391104
- 4. Stukey JE, McDonough VM, Martin CE (1989) Isolation and characterization of OLE1, a gene affecting fatty acid desaturation from Saccharomyces cerevisiae. J Biol Chem 264: 16537–16544.JE StukeyVM McDonoughCE Martin1989Isolation and characterization of OLE1, a gene affecting fatty acid desaturation from Saccharomyces cerevisiae.J Biol Chem2641653716544
- 5. Nakamura MT, Nara TY (2004) Structure, function, and dietary regulation of delta6, delta5, and delta9 desaturases. Annu Rev Nutr 24: 345–376.MT NakamuraTY Nara2004Structure, function, and dietary regulation of delta6, delta5, and delta9 desaturases.Annu Rev Nutr24345376
- 6. Miyazaki M, Bruggink SM, Ntambi JM (2006) Identification of mouse palmitoyl-CoA delta 9 desaturase. J Lipid Res 47: 700–704.M. MiyazakiSM BrugginkJM Ntambi2006Identification of mouse palmitoyl-CoA delta 9 desaturase.J Lipid Res47700704
- 7. Ntambi JM, Miyazaki M, Stoehr JP, Lan H, Kendziorski CM, et al. (2002) Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proc Natl Acad Sci U S A 99: 11482–11486.JM NtambiM. MiyazakiJP StoehrH. LanCM Kendziorski2002Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity.Proc Natl Acad Sci U S A991148211486
- 8. Miyazaki M, Dobrzyn A, Elias PM, Ntambi JM (2005) Stearoyl-CoA desaturase-2 gene expression is required for lipid synthesis during early skin and liver development. Proc Natl Acad Sci U S A 102: 12501–12506.M. MiyazakiA. DobrzynPM EliasJM Ntambi2005Stearoyl-CoA desaturase-2 gene expression is required for lipid synthesis during early skin and liver development.Proc Natl Acad Sci U S A1021250112506
- 9. Wang J, Yu L, Schmidt RE, Su C, Huang X, et al. (2005) Characterization of HSCD5, a novel human stearoyl-CoA desaturase unique to primates. Biochem Biophys Res Commun 332: 735–742.J. WangL. YuRE SchmidtC. SuX. Huang2005Characterization of HSCD5, a novel human stearoyl-CoA desaturase unique to primates.Biochem Biophys Res Commun332735742
- 10. Zhang L, Ge L, Parimoo S, Stenn K, Prouty SM (1999) Human stearoyl-CoA desaturase: Alternative transcripts generated from a single gene by usage of tandem polyadenylation sites. Biochem J 340: 255–264.L. ZhangL. GeS. ParimooK. StennSM Prouty1999Human stearoyl-CoA desaturase: Alternative transcripts generated from a single gene by usage of tandem polyadenylation sites.Biochem J340255264
- 11. Tiku PE, Gracey AY, Macartney AI, Beynon RJ, Cossins AR (1996) Cold-induced expression of delta 9-desaturase in carp by transcriptional and posttranslational mechanisms. Science 271: 815–818.PE TikuAY GraceyAI MacartneyRJ BeynonAR Cossins1996Cold-induced expression of delta 9-desaturase in carp by transcriptional and posttranslational mechanisms.Science271815818
- 12. Tabor DE, Kim JB, Spiegelman BM, Edwards PA (1998) Transcriptional activation of the stearoyl-CoA desaturase 2 gene by sterol regulatory element-binding protein/adipocyte determination and differentiation factor 1. J Biol Chem 273: 22052–22058.DE TaborJB KimBM SpiegelmanPA Edwards1998Transcriptional activation of the stearoyl-CoA desaturase 2 gene by sterol regulatory element-binding protein/adipocyte determination and differentiation factor 1.J Biol Chem2732205222058
- 13. Brown MS, Goldstein JL (1997) The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89: 331–340.MS BrownJL Goldstein1997The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor.Cell89331340
- 14. Chawla A, Repa JJ, Evans RM, Mangelsdorf DJ (2001) Nuclear receptors and lipid physiology: Opening the X-files. Science 294: 1866–1870.A. ChawlaJJ RepaRM EvansDJ Mangelsdorf2001Nuclear receptors and lipid physiology: Opening the X-files.Science29418661870
- 15. Gulick T, Cresci S, Caira T, Moore DD, Kelly DP (1994) The peroxisome proliferator-activated receptor regulates mitochondrial fatty acid oxidative enzyme gene expression. Proc Natl Acad Sci U S A 91: 11012–11016.T. GulickS. CresciT. CairaDD MooreDP Kelly1994The peroxisome proliferator-activated receptor regulates mitochondrial fatty acid oxidative enzyme gene expression.Proc Natl Acad Sci U S A911101211016
- 16. Miller CW, Ntambi JM (1996) Peroxisome proliferators induce mouse liver stearoyl-CoA desaturase 1 gene expression. Proc Natl Acad Sci U S A 93: 9443–9448.CW MillerJM Ntambi1996Peroxisome proliferators induce mouse liver stearoyl-CoA desaturase 1 gene expression.Proc Natl Acad Sci U S A9394439448
- 17. Michalik L, Desvergne B, Wahli W (2004) Peroxisome-proliferator-activated receptors and cancers: Complex stories. Nat Rev Cancer 4: 61–70.L. MichalikB. DesvergneW. Wahli2004Peroxisome-proliferator-activated receptors and cancers: Complex stories.Nat Rev Cancer46170
- 18. Watts JL, Browse J (2002) Genetic dissection of polyunsaturated fatty acid synthesis in Caenorhabditis elegans. Proc Natl Acad Sci U S A 99: 5854–5859.JL WattsJ. Browse2002Genetic dissection of polyunsaturated fatty acid synthesis in Caenorhabditis elegans.Proc Natl Acad Sci U S A9958545859
- 19. Watts JL, Browse J (2000) A palmitoyl-CoA-specific delta9 fatty acid desaturase from Caenorhabditis elegans. Biochem Biophys Res Commun 272: 263–269.JL WattsJ. Browse2000A palmitoyl-CoA-specific delta9 fatty acid desaturase from Caenorhabditis elegans.Biochem Biophys Res Commun272263269
- 20. Kahn-Kirby AH, Dantzker JL, Apicella AJ, Schafer WR, Browse J, et al. (2004) Specific polyunsaturated fatty acids drive TRPV-dependent sensory signaling in vivo. Cell 119: 889–900.AH Kahn-KirbyJL DantzkerAJ ApicellaWR SchaferJ. Browse2004Specific polyunsaturated fatty acids drive TRPV-dependent sensory signaling in vivo.Cell119889900
- 21. Watts JL, Phillips E, Griffing KR, Browse J (2003) Deficiencies in C20 polyunsaturated fatty acids cause behavioral and developmental defects in Caenorhabditis elegans fat-3 mutants. Genetics 163: 581–589.JL WattsE. PhillipsKR GriffingJ. Browse2003Deficiencies in C20 polyunsaturated fatty acids cause behavioral and developmental defects in Caenorhabditis elegans fat-3 mutants.Genetics163581589
- 22. Ashrafi K, Chang FY, Watts JL, Fraser AG, Kamath RS, et al. (2003) Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 421: 268–272.K. AshrafiFY ChangJL WattsAG FraserRS Kamath2003Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes.Nature421268272
- 23. Murphy CT, McCarroll SA, Bargmann CI, Fraser A, Kamath RS, et al. (2003) Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature 424: 277–283.CT MurphySA McCarrollCI BargmannA. FraserRS Kamath2003Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans.Nature424277283
- 24. Van Gilst MR, Hadjivassiliou H, Jolly A, Yamamoto KR (2005) Nuclear hormone receptor NHR-49 controls fat consumption and fatty acid composition in C. elegans. PLoS Biol 3: e53.. MR Van GilstH. HadjivassiliouA. JollyKR Yamamoto2005Nuclear hormone receptor NHR-49 controls fat consumption and fatty acid composition in C. elegans.PLoS Biol3e53.DOI: 10.1371/journal.pbio.0030053. DOI: 10.1371/journal.pbio.0030053.
- 25. Van Gilst MR, Hadjivassiliou H, Yamamoto KR (2005) From the cover: A Caenorhabditis elegans nutrient response system partially dependent on nuclear receptor NHR-49. Proc Natl Acad Sci U S A 102: 13496–13501.MR Van GilstH. HadjivassiliouKR Yamamoto2005From the cover: A Caenorhabditis elegans nutrient response system partially dependent on nuclear receptor NHR-49.Proc Natl Acad Sci U S A1021349613501
- 26. Miyabayashi T, Palfreyman MT, Sluder AE, Slack F, Sengupta P (1999) Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans. Dev Biol 215: 314–331.T. MiyabayashiMT PalfreymanAE SluderF. SlackP. Sengupta1999Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans.Dev Biol215314331
- 27. Robinson-Rechavi M, Maina CV, Gissendanner CR, Laudet V, Sluder A (2005) Explosive lineage-specific expansion of the orphan nuclear receptor HNF4 in nematodes. J Mol Evol 60: 577–586.M. Robinson-RechaviCV MainaCR GissendannerV. LaudetA. Sluder2005Explosive lineage-specific expansion of the orphan nuclear receptor HNF4 in nematodes.J Mol Evol60577586
- 28. Sampath H, Ntambi JM (2005) Polyunsaturated fatty acid regulation of genes of lipid metabolism. Annu Rev Nutr 25: 317–340.H. SampathJM Ntambi2005Polyunsaturated fatty acid regulation of genes of lipid metabolism.Annu Rev Nutr25317340
- 29. Wienholds E, van Eeden F, Kosters M, Mudde J, Plasterk RH, et al. (2003) Efficient target-selected mutagenesis in zebrafish. Genome Res 13: 2700–2707.E. WienholdsF. van EedenM. KostersJ. MuddeRH Plasterk2003Efficient target-selected mutagenesis in zebrafish.Genome Res1327002707
- 30. Shanklin J, Whittle E, Fox BG (1994) Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 33: 12787–12794.J. ShanklinE. WhittleBG Fox1994Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase.Biochemistry331278712794
- 31. Tanaka T, Ikita K, Ashida T, Motoyama Y, Yamaguchi Y, et al. (1996) Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans. Lipids 31: 1173–1178.T. TanakaK. IkitaT. AshidaY. MotoyamaY. Yamaguchi1996Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans.Lipids3111731178
- 32. Houthoofd K, Braeckman BP, Lenaerts I, Brys K, De Vreese A, et al. (2002) Axenic growth up-regulates mass-specific metabolic rate, stress resistance, and extends life span in Caenorhabditis elegans. Exp Gerontol 37: 1371–1378.K. HouthoofdBP BraeckmanI. LenaertsK. BrysA. De Vreese2002Axenic growth up-regulates mass-specific metabolic rate, stress resistance, and extends life span in Caenorhabditis elegans.Exp Gerontol3713711378
- 33. Dillin A (2003) The specifics of small interfering RNA specificity. Proc Natl Acad Sci U S A 100: 6289–6291.A. Dillin2003The specifics of small interfering RNA specificity.Proc Natl Acad Sci U S A10062896291
- 34. Wood WB (1988) The Nematode Caenorhabditis elegans. Cold Spring Harbor (New York): Cold Spring Harbor Laboratory Press. pp. 587–606.WB Wood1988The Nematode Caenorhabditis elegansCold Spring Harbor (New York)Cold Spring Harbor Laboratory Presspp.587606 pp.
- 35. Kamath RS, Ahringer J (2003) Genome-wide RNAi screening in Caenorhabditis elegans. Methods 30: 313–321.RS KamathJ. Ahringer2003Genome-wide RNAi screening in Caenorhabditis elegans.Methods30313321
- 36. Kamath RS, Martinez-Campos M, Zipperlen P, Fraser AG, Ahringer J (2001) Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol 2: 2.RS KamathM. Martinez-CamposP. ZipperlenAG FraserJ. Ahringer2001Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans.Genome Biol22
- 37. Wong ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. Biotechniques 39: 75–85.ML WongJF Medrano2005Real-time PCR for mRNA quantitation.Biotechniques397585
- 38. Hobert O (2002) PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans. Biotechniques 32: 728–730.O. Hobert2002PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans.Biotechniques32728730
- 39. Mello CC, Kramer JM, Stinchcomb D, Ambros V (1991) Efficient gene transfer in C.elegans: Extrachromosomal maintenance and integration of transforming sequences. Embo J 10: 3959–3970.CC MelloJM KramerD. StinchcombV. Ambros1991Efficient gene transfer in C.elegans: Extrachromosomal maintenance and integration of transforming sequences.Embo J1039593970
- 40. Clark SG, Lu X, Horvitz HR (1994) The Caenorhabditis elegans locus lin-15, a negative regulator of a tyrosine kinase signaling pathway, encodes two different proteins. Genetics 137: 987–997.SG ClarkX. LuHR Horvitz1994The Caenorhabditis elegans locus lin-15, a negative regulator of a tyrosine kinase signaling pathway, encodes two different proteins.Genetics137987997
- 41. Apfeld J, Kenyon C (1998) Cell nonautonomy of C. elegans daf-2 function in the regulation of diapause and life span. Cell 95: 199–210.J. ApfeldC. Kenyon1998Cell nonautonomy of C. elegans daf-2 function in the regulation of diapause and life span.Cell95199210
- 42. Wicks SR, Yeh RT, Gish WR, Waterston RH, Plasterk RH (2001) Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map. Nat Genet 28: 160–164.SR WicksRT YehWR GishRH WaterstonRH Plasterk2001Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map.Nat Genet28160164