To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately 4,700 viable yeast deletion mutants and determined the fitness of the double mutants. Fifty-six DNA2-interacting genes were identified. Clustering analysis of genomic synthetic lethality profiles of each of 43 of the DNA2-interacting genes defines a network (consisting of 322 genes and 876 interactions) whose topology provides clues as to how replication proteins coordinate regulation and repair to protect genome integrity. The results also shed new light on the functions of the query gene DNA2, which, despite many years of study, remain controversial, especially its proposed role in Okazaki fragment processing and the nature of its in vivo substrates. Because of the multifunctional nature of virtually all proteins at the replication fork, the meaning of any single genetic interaction is inherently ambiguous. The multiplexing nature of the current studies, however, combined with follow-up supporting experiments, reveals most if not all of the unique pathways requiring Dna2p. These include not only Okazaki fragment processing and DNA repair but also chromatin dynamics.
Maintenance of genome stability from generation to generation is a primary defense against mutation and ensuing disease. Thus, the cell has evolved complex mechanisms, consisting of redundant, partially overlapping pathways, to protect the fidelity of genome inheritance. Using modern genetic screening techniques that allow one to investigate every gene in yeast that might be involved in these pathways, the researchers have defined a network consisting of 322 genes that together safeguard the DNA replication process. Previous approaches were limited to defining the interaction of one or a few genes, but the availability of mutants affecting all of the nonessential yeast genes allowed the identification of over 800 interactions in this study. In addition, the synthetic genetic array technique used in this study allowed identification of every nonessential gene in yeast that interacts with an essential replication protein, Dna2p. The comprehensiveness of the approach identified most, if not all, of the pathways in which the multitasking Dna2p participates, in a single experiment. The genomic scale of the study significantly accelerates understanding of this protein over traditional, low-throughput genetic methods.
Citation: Budd ME, Tong AHY, Polaczek P, Peng X, Boone C, Campbell JL (2005) A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability. PLoS Genet 1(6): e61. doi:10.1371/journal.pgen.0010061
Editor: Michael Snyder, Yale University, United States of America
Received: August 3, 2005; Accepted: October 12, 2005; Published: December 2, 2005
Copyright: © 2005 Budd et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: DSB, double-strand break; FAA, 2-amino-5-fluorobenzoic acid; HU, hydroxyurea; MMS, methyl methane sulfonate; OFP, Okazaki fragment processing; pol, DNA polymerase; RFB, replication fork barrier; SGA, synthetic genetic array; ts, temperature-sensitive; WT, wild-type; YPD, yeast-peptone-dextrose
In order to preserve the fidelity of genome duplication during DNA replication, cells with complex genomes have evolved a network of pathways composed of the DNA replication apparatus, DNA repair proteins, and regulatory activities. Despite years of general characterization, knowledge of the specific mechanisms by which these pathways are integrated to protect the genome is still incomplete because of the complexity of underlying replication fork processes and their regulation. The challenge in understanding high fidelity genome transmission has progressed from identification and characterization of the individual DNA replication components to investigation of how they combine to form pathways orchestrating repair and regulation.
The first line of defense against genome instability resides with the enzymes of the DNA replication apparatus itself. While the most familiar example is the proofreading activity found in the DNA polymerases, other proteins of the replisome have also evolved substrate specificities to address errors made during replication fork progression. One of these proteins is Dna2p, a helicase/nuclease. The dna2–1 mutation was identified in a screen for yeast mutants defective in DNA replication based on an assay using permeabilized cells . dna2–1 strains were then shown to accumulate subgenomic-size DNA fragments when incubated at the restrictive temperature . Dna2p has both DNA helicase and single-stranded nuclease activities [3,4]. Biochemical and genetic characterization has revealed that Dna2p is involved in the processing of some, but not all, Okazaki fragments. Specifically, it has been proposed that Dna2p acts with FEN1 to remove RNA primers from Okazaki fragments whose 5′ RNA/DNA termini have been extensively displaced by DNA polymerase (pol) δ. Four lines of evidence support a role for Dna2p in Okazaki fragment processing (OFP). First, Dna2p co-purifies with FEN1, which is a structure-specific nuclease required for OFP in the SV40 in vitro replication system [5,6]. Second, overexpression of the Saccharomyces cerevisiae FEN1 gene, RAD27, suppresses the temperature-sensitive (ts) growth defect of a dna2–1 strain, and furthermore, the dna2–1 rad27Δ double mutant is synthetically lethal [7,8]. Third, biochemical reconstitution experiments have shown that excessive strand displacement by pol δ creates long 5′ flaps that are cleaved inefficiently by FEN1, and that initial cleavage of these flaps by Dna2p potentiates more efficient subsequent cleavage by FEN1 [9–13]. Finally, Dna2p prefers to act on flaps with secondary structures in vitro, i.e., hairpins or fold-backs containing CTG repeats, which is probably where helicase functionality becomes necessary . Thus, the Dna2p nuclease has evolved the ideal mechanism for highly specific action at a replication fork, requiring the presence of an unpaired 5′ terminus for activity, and displaying a complete lack of activity for single-stranded gaps in duplex DNA, which would result in recombinogenic double-strand breaks (DSBs). Nevertheless, there is a real question of whether the excessively displaced flaps used to study Dna2p in vitro ever occur in vivo, and some biochemical evidence suggests this may not be the case. Therefore, although biochemical data indicate that Dna2p is required for OFP when FEN1 or pol δ activity is impaired, identification of Dna2p's in vivo substrates requires more appropriate genetic and physiological assays than have been applied to date .
The second and third lines of defense for preventing genome instability during DNA replication are the DNA repair pathways and regulatory pathways, such as cell cycle checkpoints. Increasing evidence suggests that these pathways are integrated into the replication pathway through their use of certain replication proteins . Dna2p seems to be one of these multitasking proteins. Besides its function in OFP, our evidence strongly suggests that Dna2p provides a link between DNA replication and DNA repair, since dna2 mutants are sensitive to methyl methane sulfonate (MMS), X-rays, bleomycin, and hydroxyurea (HU) [8,16,17].
To provide a comprehensive view of the roles of Dna2p at the replication fork and in other genome maintenance pathways, we conducted a large-scale synthetic lethal screen by synthetic genetic array (SGA) analysis using DNA2 as a query [18,19]. Two genes are synthetically lethal if single mutants, defective in either gene, are viable, while double mutants, defective in both genes, are inviable. Two mutants are synthetically sick if the double mutant grows significantly slower than either single mutant. Synthetic lethality is useful for identifying redundancy and complementarity, e.g., pathways that compensate for functional deficiencies in each other or genes encoding products that are both required to efficiently process a common substrate, which is often the case for DNA replication and repair proteins. Synthetic lethal screens not only reveal previously unknown genetic interactions with queried mutants but also how gene products and their corresponding pathways functionally associate.
Our results provide a catalogue of most, if not all, pathways that are interdependent with or require Dna2p, thus revealing both the extent and limits of its multitasking character. The work not only confirms a role in OFP, but also identifies functions in (1) a replication/repair helicase subnet, (2) DSB repair and mismatch repair, (3) the replication stress checkpoint, (4) sister chromatid cohesion, (5) chromatin dynamics, (6) histone modification, and (7) osmotic and oxidative stress responses. In a more general sense, the interactions link a specific network of DNA repair and regulatory pathways to a specific network of replication genes that together maintain high fidelity lagging-strand DNA replication.
Since DNA2 is an essential gene, either a conditional or hypomorphic allele is required for a synthetic lethal screen. We chose two alleles, dna2–1, a ts mutant sensitive to a variety of DNA damaging agents, and dna2–2, a mutant that grows at 23 °C and 37 °C, but is sensitive to MMS, bleomycin, and X-rays [8,20]. dna2–1 contains a P504S substitution in a region of the protein N-terminal to both the nuclease and helicase domains . All enzymatic activities of the Dna2–1 protein are reduced relative to wild-type (WT)—DNA-stimulated ATPase, DNA helicase, and single-stranded DNA nuclease activity . The dna2–2 mutation changes arginine at position 1235, an invariant residue in helicase region IV, to glutamine . A crystal structure of PCR helicase shows that residues in region IV bind the adenine base of ATP . The dna2–1 strain grows very slowly, even at the permissive temperature, so synthetic sickness is sometimes difficult to unambiguously assign for this mutant. The dna2–2 mutant grows faster than the dna2–1 mutant, and synthetic sickness, characterized by slow growth, can be assigned with greater confidence using dna2–2 strains. The use of each dna2 allele in a separate SGA screen expands the range of detectable synthetic lethal interactions.
Table 1 lists the validated synthetic lethal and synthetic sick interactions of dna2–1 and dna2–2 strains obtained from the SGA screen (see Materials and Methods). Validation was performed by preparing a new heterozygous diploid between the respective dna2 allele and the candidate gene, followed by sporulation and tetrad dissection. The dna2-allele specificity of some of the interactions may turn out to be significant, as the mutations differentially affect helicase and nuclease activity. Mutants that appeared to show growth defects in the primary screen but that did not meet the stringent requirements (see Materials and Methods) for interaction imposed by the secondary tetrad analysis are found in Table S1. Mutants synthetically lethal or synthetically sick with either dna2–1 or dna2–2 fall into the following categories: genes involved in OFP (rad27Δ, exo1Δ, yen1Δ, rnh35Δ, rnh202Δ, pol3–01, rpa1, elg1Δ, pol1, and pri1), nonessential helicases involved in maintaining chromosome stability (sgs1Δ, srs2Δ, and rrm3Δ), genes involved in repair (rad52Δ, mre11Δ, rad50Δ, xrs2Δ, sae2Δ, mms1Δ, mms22Δ, slx5, and slx8), genes involved in the DNA replication checkpoint (mrc1Δ, csm3, and tof1Δ), genes involved in chromosomal cohesion (ctf4Δ and ctf18Δ), genes involved in chromatin disassembly/assembly and nucleosome modification and remodeling (spt16, pob3, rad6Δ, bre1Δ, swd1Δ, swd3Δ, hst3Δ, rpd3Δ, pho23Δ, rtf1Δ, and the histone chaperone asf1Δ), genes involved in the oxidative stress response (lys7Δ and sod1Δ), a gene in the osmotic stress response (hog1Δ), and genes involved in degradation of short-lived proteins (ubc4Δ), in polarized cell growth (cla4Δ), and in mRNA processing (trf4Δ and rtt103Δ). Previous studies showed dna2 to be synthetically lethal with several essential genes: mcm10–1, involved in initiation of replication ; cdc9, a DNA ligase (, although see also ); rpa1, a single-stranded DNA binding protein ; and spt16 and pob3, two genes involved in chromosome remodeling .
Genes Identified as Putative DNA2 Interacters from the SGA Screen with dna2–1 and dna2–2 Queries and Verified by Tetrad Dissection
The total number of genes that interact with dna2 to date is 56, a connectivity similar to that found in previous screens . Figure 1 shows two-dimensional hierarchical clustering of the data from the current screen and that of previous screens using 43 genes that interact with DNA2 as queries . Figure 2 provides a graphical representation of the network (consisting of 322 genes and 876 interactions) in which DNA2, RAD27, SGS1, SRS2 (HPR5), RRM3, and POL32 form the six major hubs (largest number of connections). We were surprised that none of these genes showed interactions with cell cycle genes. ctf4Δ, however, which is synthetically lethal with all six of these hub genes and many of the other nodes as well (Figure 2), is synthetically lethal with clb2Δ, clb3Δ, clb5Δ, mad1Δ, mad2Δ, mad3Δ, bub1Δ, bub2Δ, and bub3Δ . Thus, CTF4 may provide a missing link between hub genes and cell cycle and kinetochore functions. The same information is shown in tabular form in Table S2, which contains the interacting genes organized by functional categories defined in the MIPS database (http://mips.gsf.de/genre/proj/yeast/index.jsp). Mutations (identified in studies reported below) that suppress dna2 alleles are also included. We propose that the genes showing the greatest number of interactions encode products that share at least one substrate or have at least one overlapping function.
Hierarchical two-dimensional clustering analysis was applied to the DNA2 interactions and those of 43 genes synthetically lethal with DNA2 (see Table 1). The interactions were clustered with respect to the results of the current screen and 43 previous screens using these genes as queries. A total of 322 genes and 876 interactions, each indicated by a red box, were identified. This panel shows a zoom into the region of most significant overlap of shared genetic interactions. In addition to including reduced fitness genes, this analysis includes genes that give increased fitness with dna2, such as pol32Δ.
Data were compiled with the Payek program using the dna2 screen results in Table 1 and the results of the previous screens [18,19,92], as well as data compiled for candidate genes synthetically lethal with rrm3Δ [47,75]. The data are presented in tabular form in Table S2.
Okazaki Fragment Processing
Although Dna2p is commonly considered to be required for OFP, this role is far from established. We were therefore interested to find that the SGA experiments identified interactions between DNA2 and additional structure-specific nucleases that, like FEN1, are thought to be involved in overlapping OFP pathways. First, dna2–1 was found to be synthetically lethal with mutations in genes encoding two subunits, rnh35Δ and rnh202Δ, of the main RNaseH in yeast , suggesting that Dna2p may be involved in an OFP pathway redundant with RNaseH2.
Second, dna2–2 was synthetically lethal with mutations in genes encoding two exo-endonucleases structurally related to RAD27, exo1Δ and yen1Δ. EXO1 is thought to provide backup function for FEN1 in OFP, since rad27Δ exo1Δ is synthetically lethal (in some genetic backgrounds) and since overexpression of EXO1 suppresses the ts growth of rad27Δ mutants [26–28]. Overexpression of EXO1 also suppressed the temperature sensitivity of dna2–1 at the restrictive temperature of 30 °C (Figure 3), though not at 37 °C . Exo1p nuclease acts on 5′ flap-containing structures, and these genetic interactions suggest that such flaps may be the common in vivo substrate of both Dna2p and Exo1p .
Strain W303 dna2–1 was transformed with the plasmids pRS424G and pRS424G-EXO1, an empty plasmid vector and a plasmid expressing ExoIp from the GAL110 promoter, respectively. W303RAD5 strains dna2–1 7A(pRS424G) and dna2–1(pRS424G-EXO1) were grown to mid log phase and serially diluted on yeast-dextrose galactose- and raffinose-containing plates and incubated at 30 °C for 5 d.
We also found that dna2–2 is synthetically sick with yen1Δ at 30 °C and lethal, i.e., ts, at 37 °C. Yen1p shows 23% identity with Rad27p, 33% identity with Rad2p (founding member of this nuclease family and involved in nucleotide excision repair), and 24% identity with Din7p (Rad2p-like endonuclease proposed to be involved in mitochondrial mismatch repair). yen1Δ mutants do not appear to have DNA damage or growth defects, and a yen1Δ exo1Δ din7Δ rad2Δ mutant has no growth defect at either 30 °C or 37 °C . Synthetic lethality with dna2 is the first informative phenotype reported for yen1Δ, although yen1 is synthetically lethal with another gene that is in turn synthetically lethal with several replication mutants (see Protocol S1).
Elg1p, like Dna2p, is proposed to be involved in OFP, as well as in telomere silencing and length regulation [17,30,31]. elg1Δ dna2–1 mutants are synthetically lethal (Table 1). Elg1p is homologous to Rfc1p (the large subunit of the replication factor C clamp loader), to Rad24p (a Rfc1p homolog required for DNA damage checkpoints), and to Ctf18p (another Rfc1p homolog involved in chromosomal cohesion). dna2–2 is also synthetically lethal with ctf18Δ , but not with rad24Δ (this work). S phase progression in elg1Δ mutants is slower than in WT strains, suggesting that Elg1p is involved in replication fork translocation [30–32].
Interaction of dna2 with Pol δ
It has been proposed, based on in vitro biochemical reconstitution in vitro, that not all Okazaki fragments require Dna2p for processing. Instead, the in vivo substrates of Dna2p are only those Okazaki fragments on which pol δ produces 5′ flaps longer than 30 nucleotides [10,12,14,33]. We wished to devise a genetic test of this hypothesis. One way to do so is by generating excessive strand displacement in vivo and assessing whether there is then an increased requirement for DNA2. This was accomplished by testing the viability of a strain containing both the dna2–1 mutation and pol3–01, a mutation in pol δ known to increase strand displacement in vitro . As shown in Figure 4, dna2–1 pol3–01 is synthetically lethal. We propose that excessive strand displacement in vivo in the pol3–01 dna2–1 strain causes the lethality, supporting the predictions from purely biochemical reconstitution.
Strain W303 dna2–1 carrying a TRP1 CEN3 DNA2 plasmid was transformed with an integrating URA3 pol3–01 plasmid  cut with BamH1. The transformants were streaked on 5-FOA medium to excise the WT POL3 gene and identify clones with the pol3–01 mutation. Three transformants carrying the pol3–01 mutation (isolates 2, 8, and 11) were restreaked on YPD medium containing 2-amino-5-fluorobenzoic acid (FAA), which selects for strains that have lost the DNA2 TRP1–containing plasmid . Three dna2–1 pol3–01 colonies showing some residual growth on the FAA plates were restreaked on YPD-containing medium. The same three isolates of dna2–1 pol3–01 but containing the DNA2 TRP plasmid, i.e., that had not been grown in the presence of FAA, are shown as controls, as indicated.
Another pol δ subunit mutant, pol32Δ, is synthetically lethal with rad27Δ, and the network of synthetic lethal interactions of pol32Δ is similar to that of rad27Δ, rrm3Δ, sgs1Δ, and srs2Δ, which are also synthetically lethal with dna2 (See Figure 2). We were surprised that pol32Δ did not show up in our SGA screen, and directly tested dna2–1 pol32Δ for synthetic lethality. Rather than showing synthetic lethality, pol32Δ suppressed the slow growth phenotype of the dna2–1 strain at 23 °C and suppressed the ts growth phenotype of the dna2–1 strain at 30 °C (Figure 5A). The pol32Δ mutation did not suppress the lethality of the dna2–1 strain at 37 °C (Figure 5A) or the lethality of a DNA2 deletion (not shown). The pol32Δ deletion mutation also suppressed the DNA damage sensitivity of the dna2–1 and dna2–2 mutants (Figure 5B and 5C, respectively). pol32Δ mutants are sensitive to different concentrations of MMS than are dna2 mutants. dna2–1 and dna2–2 mutants are sensitive to 0.005% MMS, while pol32Δ strains are resistant to 0.005% MMS but are sensitive to 0.01% MMS. Both dna2–1 pol32Δ and dna2–2 pol32Δ mutants showed MMS sensitivity similar to pol32Δ strains rather than to dna2–1 or dna2–2 strains (Figure 5B and 5C). Pol32p is a nonessential subunit of the pol δ holoenzyme and is required for full processivity of pol δ . Therefore, a less processive pol δ suppresses the growth and repair defects of both nuclease- and helicase-deficient mutants of dna2, suggesting that Dna2p is acting on flaps arising from excessive strand displacement by pol δ during DNA repair as well as during OFP. The synthetic lethality of dna2 and pol3–01 and the suppression of dna2–1 by pol32Δ provide strong genetic evidence that the Dna2p substrates shown to be optimal in vitro are also substrates in vivo (see Discussion).
(A) WT, pol32Δ, dna2–1 pol32Δ, and dna2–1 strains were grown to log phase, serially diluted, and plated on YPD plates and incubated at 23 °C, 30 °C, and 37 °C for 5 d.
(B) WT, pol32Δ, dna2–2, and pol32Δ dna2–2 strains were grown to log phase, serially diluted, and incubated on MMS-containing YPD plates for 3 d at 30 °C.
(C) WT, pol32Δ, dna2–1 pol32Δ, and dna2–1 strains were grown to log phase, serially diluted, and grown on MMS-containing YPD plates for 5 d at 23 °C.
All strains are isogenic with strain 4741 (Table S4). (dna2–1 grows slowly even at 23 °C, and plates at 23 °C were photographed before they were fully grown so that the other strains would not be overgrown.)
The Helicase Network
We have reported previously that dna2–2 is synthetically lethal with sgs1Δ, srs2Δ, and rrm3Δ [35–37], and our SGA screen also detected these genes. More significantly, there is extensive overlap between genes that are synthetically lethal with dna2 and those that are synthetically lethal with sgs1Δ and srs2Δ, as shown in Figures 1 and 2.
To assess the functional relationship between Dna2p and these helicases, we have tried to further determine whether the synthetic lethality between dna2 and the helicase genes shown in Table 2 stems from defects in DNA replication per se or from the aberrant DNA structures that arise during repair of DNA replication errors. Towards this end, we first checked whether the lethality was suppressed by mutations in genes considered necessary for resolving potentially lethal intermediates that form when the original lesions enter the recombination pathway for repair . The results of our current analysis of dna2–2 sgs1Δ, dna2–2 srs2Δ, and dna2–2 rrm3Δ are summarized in Table 2. dna2–2 sgs1Δ and dna2–2 srs2Δ were synthetically lethal and were either inefficiently or efficiently suppressed, respectively, by rad51Δ, as we reported previously ([35,37]; see Discussion). New here is the finding that dna2–2 rrm3Δ lethality was not suppressed by mutations in the recombination pathway (Table 2). This indicates that cell death in dna2–2 rrm3Δ does not result from the accumulation of nonresolvable recombination intermediates but from the formation of early replication intermediates, or possibly blocked DNA replication forks.
Genetic Interactions of DNA2 with Other Helicases
Gene pairs encoding three different heterodimeric complexes (MMS4/SLX3, SLX1/4, and SLD5/8), although they are not DNA helicases, are required for viability in the absence of SGS1 and, thus, are part of the helicase network . We find that only one of these complexes, defined by slx5Δ and slx8Δ, shows synthetic lethal interactions with dna2. Since the function of the Slx5/8p complex is unknown, we cannot yet predict the nature of the substrates that might be shared with Dna2p.
We have previously shown that dna2 and rad52Δ mutants are synthetically sick . rad52Δ also appeared synthetically sick with the dna2 alleles in the SGA screen. We were surprised that other genes required for recombinational repair did not appear synthetically lethal with dna2 in the SGA screen, since they are synthetically lethal with rad27Δ. To insure that we had not missed such genes, dna2–1 rad51Δ and dna2–1 rad55Δ, as well as dna2–2 rad51Δ and dna2-2 rad59Δ, mutants were constructed. Consistent with the global screen, these double mutants did not appear to be synthetically lethal or sick. We also found that dna2–2 rad51Δ rad59Δ triple mutants were viable (not shown). Our results indicate that the Rad51p/Rad59p portion of the Rad52p pathway does not buffer Dna2p function. MMS1 and MMS22 are two additional presumed DNA repair genes that are also required for normal S phase progression and that show synthetic lethality with dna2 mutants in the SGA screen, as previously described . The Mms1p/Mms22p system may correspond to the pathway that results in dna2–2 rad52Δ growth defects.
Previously, we reported that dna2–2 and rad50–5 double mutants were viable and epistatic for repair . Therefore, one of our most unexpected findings was that dna2 mre11Δ and dna2 rad50Δ strains are synthetically lethal (see Table 1). rad50–5 is a point mutation of RAD50 that is as sensitive to irradiation as either a rad50Δ or a rad52Δ strain. Clearly, some function must remain in the point mutant as compared to the deletion mutant, however, since the dna2–2 rad50–5 strain is viable while the dna2–2 rad50Δ strain is inviable. MRE11, RAD50, and XRS2 encode members of the Mre11p complex, which is required for the intra-S phase checkpoint, for homologous recombination, for non-homologous end joining, and for telomere maintenance ([41,42] and references therein). Although xrs2Δ was not found in our SGA screen, we have since shown that dna2–2 xrs2Δ is synthetically lethal (this work).
dna2 Mutants Do Not Require the rad9, mrc1, mec1, or tel1 Checkpoint Activities for Viability
Our analysis of the interaction between dna2 and checkpoint mutants supports the view that dna2–1 and dna2–2 mutants accumulate less damage than rad27Δ mutants. First, we found that neither dna2 allele used in our screen is synthetically lethal with rad9, a mediator of the intra-S DNA damage checkpoint, as found previously [8,43] for other dna2 alleles. rad27Δ mutants are synthetically lethal with rad9Δ. In addition, rad27Δ mutants are synthetically lethal with the DNA damage checkpoint mutants rad24Δ and rad17Δ, which represent the checkpoint clamp-loader-like complex and the checkpoint clamp-like proteins, respectively, but dna2 rad24Δ and dna2 rad17Δ are both viable (this study). Thus, dna2 mutants do not accumulate sufficient damage to require the intra-S DNA damage checkpoint for viability at the permissive temperature.
We found in the SGA experiments, however, that dna2 mutants are synthetically lethal with mrc1Δ, tof1Δ, or csm3Δ (Table 1). These genes define a checkpoint pathway thought to operate in parallel with the Rad9p pathway to respond specifically to replication stress. Mrc1p, Tof1p, and Csm3p are required for Rad53p phosphorylation in the presence of HU or MMS, both of which induce S phase DNA damage . Mrc1p is an adapter molecule in the checkpoint, whose phosphorylation by Mec1p at SQ/TQ motifs is required for activation of downstream effectors [44,45]. dna2 mrc1Δ synthetic lethality seemed to contradict the observation that other dna2 alleles actually show improved survival in the absence of the checkpoint (; see below), and suggested that such synthetic lethality might be due to another function of MRC1. MRC1 and TOF1 appear to play a direct role in yeast DNA replication, as well as in the checkpoint. mrc1Δ strains also show a slow S phase, and Mrc1p and Tof1p have been localized to moving replication forks . Osborn and Elledge  constructed a separation-of-function mrc1 mutant that has all 17 TQ and SQ Mec1p target sites mutated to non-phosphorylatable AQ. This mutant, mrc1AQ, like mrc1Δ, is checkpoint defective, as evidenced by the fact that Rad53p phosphorylation is blocked in mrc1AQ rad9Δ mutants upon treatment with HU or MMS. However, mrc1AQ mutants are replication proficient. The mrc1AQ mutant allowed us to ask whether the replication defect of mrc1Δ was responsible for dna2 mrc1Δ synthetic lethality. dna2–1 mrc1AQ and dna2–2 mrc1AQ strains were constructed and were viable and did not appear synthetically sick, indicating that the checkpoint function of Mrc1p is not required for viability in dna2 mutant backgrounds (Figure 6). In order to be sure that RAD9 was not substituting for MRC1 in the dna2–2 mrc1AQ mutant, dna2–2 mrc1AQ rad9Δ mutants, which lack both the DNA damage and replication stress checkpoints, were constructed (Figure 6). The viability of dna2–2 mrc1AQ rad9Δ strains suggests that it is inactivation of the replication function of MRC1 that is responsible for synthetic lethality in the dna2 mrc1Δ mutants.
mrc1Δ experiments were carried out in the isogenic 4741 strain and are listed in Table S4. mrc1AQ experiments were carried out in strains isogenic with W303 RAD5+.
Panel 1 (top): segregants from a DNA2/dna2–2 MRC1/mrc1Δ diploid. Segregants are isogenic with 4741. d, dna2–2; m, mrc1Δ.
Panel 2: segregants from a MRC1/mrc1AQ RAD9/rad9Δ DNA2/dna2–2 diploid. d, dna2–2; m, mrc1AQ; r, rad9Δ.
Panel 3: segregants from a DNA2/dna2–1 MRC1/mrc1AQ RAD9/rad9Δ strain. d, dna2–1; m, mrc1AQ; r, rad9Δ.
Panel 4 (bottom): segregants from a RRM3/rrm3Δ MRC1/mrc1AQ diploid. m, mrc1AQ; r, rrm3.
mrc1Δ and rrm3Δ mutants are also synthetically lethal  and, because RRM3 and DNA2 interact (see Table 2), we asked whether the mrc1Δ rrm3Δ synthetic lethality is caused by a checkpoint or replication defect. The mrc1AQ rrm3Δ double mutants showed the same viability as the single mutants (Figure 6). Since dna2 and rrm3 are also synthetically lethal, this suggests that DNA2, RRM3, and MRC1 functions may be interdependent in DNA replication.
To further test the idea that S phase checkpoint signaling is dispensable for dna2 mutant viability, the interaction of dna2 with mec1Δ and tel1Δ, mutations in genes upstream of MRC1 and RAD9 in the checkpoint, were investigated. A dna2–2/DNA2 tel1Δ/TEL1 mec1Δ/MEC1 sml1Δ/SML1 heterozygote was sporulated, and Table S3 lists the genotypes obtained among the tetrads. (sml1Δ allows for mec1Δ viability.) The mec1Δ mutation partially suppressed the slow growth phenotype of dna2–2 strains. Thus, as previously observed for dna2–20 , the Mec1p-mediated checkpoint is deleterious in the dna2–2 mutant (Figure 7). The tel1Δ mutation shows negative synergy but not lethality with dna2–2 at 37 °C (Figure 7). The negative synergy between dna2–2 and tel1Δ is evidence that Dna2p and Tel1p may function together at DSBs and/or at telomeres, along with the Mre11p complex . dna2–2 tel1Δ mec1Δ triple mutants were recovered. As shown in Figure 7, however, the dna2–2 tel1Δ mec1Δ mutant grew more slowly than any of the single or double mutants. The telomere defects of the mec1Δ tel1Δ strain caused it to senesce as rapidly as est2Δ (telomerase catalytic subunit deleted) strains . The dna2–2 mutation may cause additional defects in telomere replication. Thus, enhanced telomeric senescence might account for the slow growth of the dna2–2 tel1Δ mec1Δ mutant, just as we have shown that dna2–2 est2Δ is synthetically lethal due to accelerated senescence .
Strains used in these experiments are listed in Table S4, and were isogenic or congenic with W303 RAD5+. Segregants of a MEC1/mec1Δ TEL1/tel1Δ DNA2/dna2–2 SML1/sml1Δ diploid were placed on a YPD plate incubated at 30 °C (A) or 37 °C (B).
Finally, we tested the interaction of DNA2 with the checkpoint effector kinase RAD53. We crossed dna2–1 and dna2–2 to an isogenic rad53Δ sml1Δ strain. dna2–1 rad53Δ sml1Δ and dna2–2 rad53Δ sml1Δ mutants were fully viable. The viability of dna2 rad53 strengthens the conclusion that the DNA damage arising in a dna2 mutant is not sufficient to require the S phase checkpoint for viability. (dna2–1 mutants, however, do induce an amount of damage above the threshold for checkpoint activation at restrictive temperatures, since they arrest at the metaphase to anaphase transition in a MEC1-dependent manner [M. E. B. and J. L. C., unpublished data].)
Sister Chromatid Cohesion and Repair of DSBs in the rDNA
Ctf4p is a pol α–binding protein , and ctf4Δ strains are defective in sister chromatid cohesion . dna2–2 was identified as a mutant synthetically lethal with ctf4Δ, but we have shown that dna2 mutants are not defective in cohesion . We previously reported that the dna2-2 mutation gave rise to an increased frequency of DSBs at the replication fork barrier (RFB) in the rDNA and that deleting FOB1, which is required for pausing at the RFB, suppressed DSB formation. A reasonable explanation for all of these observations is that the DSB damage sustained by dna2–2 mutants at the RFB might require Ctf4p-mediated sister chromatid cohesion for repair. If so, then one would expect fob1Δ to suppress dna2–2 ctf4Δ synthetic lethality. We dissected 55 tetrads from a dna2–2 ctf4Δ fob1Δ heterozygote and incubated the spores at 30 °C. Viable dna2–2 ctf4Δ fob1Δ mutants were obtained in the expected numbers, demonstrating suppression. Although the dna2 ctf4Δ fob1Δ triple mutants grew at 23 °C and at 30 °C, they did not grow at 37 °C and were highly sensitive to X-rays (Figure 8). The behavior of the triple mutant indicates that defects in the rDNA locus are critical for some of the phenotypes of dna2–2, but that defects elsewhere throughout the chromosome must still occur, giving rise to ts growth and DNA damage sensitivity.
Top panel: Tetrads from the dissection of a DNA2/dna2–2 CTF4/ctf4Δ FOB1/fob1Δ heterozygote. c, ctf4Δ; d, dna2Δ; f, fob1Δ; WT, DNA2 CTF4 FOB1. The following spores were recovered: 11 WT, 21 dna2–2, 26 fob1Δ, 17 ctf4Δ, 18 dna2–2 fob1Δ, 20 ctf4Δ fob1Δ, zero ctf4Δ dna2–2, and six dna2–2 ctf4Δ fob1Δ. Since the triple mutant grew slowly at 30 °C, another 27 spores were dissected and incubated at 23 °C. The following spores were recovered: 14 WT, nine fob1Δ, 14 ctf4Δ, seven dna2–2, 14 dna2–2 fob1Δ, nine ctf4Δ fob1Δ, zero dna2–2 ctf4Δ, and nine dna2–2 ctf4Δ fob1Δ. The triple mutant did not grow at 37 °C.
Bottom panel: X-ray sensitivity of dna2–2 ctf4Δ fob1Δ. Strains with genotype WT, fob1Δ, dna2–2 fob1Δ, ctf4Δ fob1Δ, and dna2–2 ctf4Δ fob1Δ were grown to log phase, irradiated as described in Materials and Methods, serially diluted, plated on YPD plates, and incubated at 30 °C. Strains are isogenic or congenic with 4741.
Nucleosome Remodeling: Dna2p Interacts with Pol α and Primase
Pob3p and Spt16p/Cdc68p form a heterodimer that is a component of the ATP-independent chromatin remodeling activity yFACT . dna2–2 is synthetically lethal with a non-ts allele of POB3, pob3–21 , and various alleles of spt16 are synthetically lethal or sick with dna2–2 . Since yFACT may participate in both DNA replication and transcription, to investigate a potential link with the role of Dna2p in replication and/or repair, we took advantage of the observation that yFACT interacts both genetically and physically with pol α [54,55]. This suggested dna2 might be synthetically lethal with a mutant containing a pol α protein that fails to interact with Pob3p/Spt16p, pol1–1 (with glycine at position 493) . We established the synthetic lethality of dna2–2 and pol1–1 (18 tetrads, 46 viable spores, no double dna2–2 pol1–1 mutants). The dna2–2 pol1–1 lethality is allele specific, since dna2–1 is not synthetically lethal with pol1–17, a catalytic site mutant  (see Discussion). Although yFACT may also affect transcription and the synthetic effects between dna2 and yFACT components could be due to reduced transcription of dna2–2 or of other replication genes in the double mutants, the synthetic lethality of dna2–2 pol1–1 argues that Dna2p and yFACT may interact during DNA replication.
We went on to investigate genetic interactions between dna2 and genes encoding other pol α subunits. We found that dna2–2 is synthetically lethal with a primase subunit mutant, pri1-M4 (18 tetrads, 51 viable spores, no double mutants). This result further implicates Dna2p in lagging-strand DNA replication, as the pri1-M4 mutant is defective in elongation .
Chromatin Remodeling and Histone Modification
In addition to clarifying the relationship between dna2 and the yFACT complex by identifying the dna2/pol1–1 interaction, the SGA screen suggested a role for Dna2p in additional complexes that control assembly and disassembly of chromatin during DNA replication. We found that dna2 mutants are synthetically lethal or synthetically sick with asf1Δ, defective in a chaperone protein involved in histone deposition at the replication fork. Since dna2 mutants are defective in OFP, they might also be defective in the rapid reassembly of chromatin behind the replication fork, leading to an accumulation of defective chromatin and lethal DSBs .
The SGA screen also revealed that dna2 mutants are synthetically lethal or sick with rad6Δ, bre1Δ, swd1Δ, and swd3Δ (see Table 1). Rad6p is a ubiquitin-conjugating enzyme (E2) required for post-replication repair, the N-end rule, and chromatin modification [60,61]. Rad6p functions with two alternative ubiquitin ligases (E3)—RAD18 in post-replication repair and BRE1 in histone H2B ubiquitylation [62–65]. dna2 rad18Δ mutants grew normally (this work). By contrast, dna2 showed synthetic sick interactions with bre1Δ and with mutations in two genes that act downstream of BRE1, swd1Δ and swd3Δ (Table 1). Bre1p-mediated histone H2B ubiquitylation is necessary for histone H3 lysine 4 methylation by Swd1p and Swd3p. These results may indicate that dna2 rad6Δ growth defects do not result from defective post-replication repair, but rather from defects in histone modification.
dna2 is also synthetically lethal with rtf1Δ, which is involved in recruitment of Set1p and H2B ubiquitylation. RTF1 is a member of the PAF1p complex. Since the PAF1p complex is involved in transcription, a trivial explanation of the dna2 rtf1Δ lethality could be that transcription of dna2 or of another replication gene is reduced and indirectly creates the synthetic lethality. If so, then dna2 should also be synthetically lethal with a paf1Δ. However, we found that no dna2 allele tested was either synthetically sick or lethal with paf1Δ. Recent evidence suggests that Rtf1p, which recruits Set1p, affects genome-wide ubiquitylation of histone H2B by a mechanism that is distinct from its function as a transcriptional elongation factor , and that would explain how we can find no effect of paf1Δ but lethality with rtf1Δ.
dna2 also shows synthetic interactions with mutations in genes encoding histone deacetylases: hst3Δ and pho23Δ. Rpd3p and Pho23p are components of a histone deacetylase complex that is thought to be involved in regulation of initiation of DNA replication [67,68], and therefore we tested dna2–2 rpd3Δ, and found that it was synthetically lethal. DNA2 is the only replication gene that has shown a genetic interaction with rpd3Δ to date (see also Protocol S1). (We wish to emphasize that for all of the chromatin-modifying pathways detected in the screen, we have shown, in studies in preparation for presentation elsewhere, that the effects are not due to the trivial explanation of reduced transcription of the mutant dna2 genes).
Additional nodes in the network (oxidative stress genes, osmotic stress genes, and genes involved in RNA modification/catabolism [mutants trf4Δ and rtt103Δ]) that are synthetically lethal with dna2 are described and discussed in Protocol S1. Genes involved in transcription elongation (CAF20, for example) have not yet been further analyzed because they may reduce transcription of Dna2p or another replication protein and thus indirectly cause lethality.
We find that 56 genes interact genetically with DNA2. Comparison of our results with those of previous synthetic lethal screens using 43 of the DNA2-interacting genes defines a set of pathways, all of which are interdependent with DNA2 and that form a network for preserving genome stability (see Figure 2). The six major hubs shown in Figure 2 link DNA replication, DNA repair, chromosome dynamics, checkpoints, chromosome structure/chromatin, osmotic stress, oxidative stress, and RNA metabolism. A major link to the cell cycle and the kinetochore occurs through a single gene, CTF4. Analysis of mutants that give rise to gross chromosomal rearrangements, the type of damage considered to be the most likely result of replication apparatus failure, identifies the same pathways [69–75]. The comprehensive nature of the SGA screen, however, allows greater insight into the structure of the network that coordinates these events. It is striking that this topology can be superimposed on the prokaryotic DNA replication interactome recently identified using protein–protein interactions . The bacterial genome maintenance network consists of many of the E. coli orthologs (i.e., pol III holoenzyme, SSB, RecQ, RecG, SbcB, and RecJ) of the yeast replicative polymerase, its subunits, and the helicases and nucleases that form hubs and major nodes in our genetic network . The common denominator in the diverse approaches was the use of a replication gene or protein as the bait. The parallels between the organisms point to evolutionary conservation in the coordination of processes that protect the genome. We suggest that the complexity of such processes was required for the evolution of large genomes, where the fidelity of the replication apparatus itself could not guarantee a sufficiently high level of accuracy and stability in genome transmission.
Current methods of scoring interactions do not result in identification, in the initial SGA screen, of every interacting gene (see discussions in ). To approach completeness, following identification of a single gene in a pathway, e.g., MRE11, in the SGA screen, we pursued “traditional” investigation, on a gene-by-gene basis, of other genes in the putative pathway, such as XRS2. Similarly, the identification of the Bre1p ubiquitylation pathway was interpreted only after testing downstream genes in the histone H2B modification pathway. This type of comprehensive genetic analysis is a powerful new tool for rapidly characterizing the full complement of processes requiring replication genes that might be coming under analysis for the first time, as well as rendering a coherent picture of years of genetic analysis of other genes. The genetic screen then enables one to rationally design experiments to determine, in molecular terms, the contribution of the replication protein to these processes. The outcome of such secondary analyses of DNA2 is discussed and interpreted below.
Stronger Links between Dna2p and OFP during Lagging-Strand Replication: RNH32, RNH202, EXO1, YEN1, POL3, POL32, POL1, and PRI1
Although it has been known for some time that dna2 and rad27Δ are synthetically lethal, convincing genetic interactions between dna2 and other lagging-strand activities have not been previously identified. As shown by our demonstration in this work of synthetic lethality of dna2 and rtf1Δ and the viability of dna2 and paf1Δ (see Results), synthetic lethality with one gene in a pathway does not prove interaction with other genes in that pathway. Therefore, the identification of so many additional lagging-strand genes in the current study is a matter of some note. The synthetic lethality of dna2 with the genes encoding structure-specific nucleases (RNH32, RNH202, EXO1, and YEN1) provides the first evidence, to our knowledge, that RNA may be a substrate of Dna2p in vivo and strengthens evidence, as predicted from our in vitro studies , that Dna2p acts primarily if not exclusively on flaps in vivo. It has been known for some time that rad27Δ rnh35Δ is synthetically sick, but not lethal . Since FEN1 is generally considered the major OFP nuclease, the more significant synthetic lethality of dna2 rnh35Δ and dna2 rnh202Δ was somewhat unexpected, and suggests that Dna2p also acts in an OFP pathway redundant with RNaseH2. Since Rnh35p does not act on flap-containing structures, the common substrate for RNaseH2 and Dna2p is probably RNA. Alternative to a role in OFP, the dna2 rnh35Δ synthetic lethality might reflect a redundant role for RNaseH2 and Dna2p in mRNA processing, an additional function proposed for RNaseH2 . rnh35Δ mutants also have very short telomeres . Since Dna2p is required for de novo telomere synthesis and dna2 est1 and dna2–2 est2 are synthetically lethal , dna2–1 rnh35Δ lethality could be related to events at telomeres.
Suppression of dna2 by EXO1 overexpression, combined with dna2 exo1Δ synthetic lethality, suggests that Exo1p can provide a backup for Dna2p in OFP, with 5′ flaps constituting the common substrate. exo1Δ is also synthetically lethal with mre11Δ, and it has been proposed that Exo1p participates in the resection of ends at DSBs in preparation for recombinational repair . Exo1p may also be involved in a late step in the Msh2p-dependent mismatch repair pathway and perhaps in telomere end processing , which might also account for the dna2 exo1Δ lethality. There is no biochemical evidence as yet to show whether Yen1p is a structure-specific 5′ to 3′ nuclease, but this is likely given its similarity to FEN1 and Exo1p. Given that Yen1p is a preferential substrate of the Clb5p cyclin-dependent kinase required for proteins that function in G1 and early S phase , the yen1Δ dna2–2 synthetic interaction may reflect a direct link between YEN1 and DNA replication (see also Protocol S1). Since there are about 100,000 Okazaki fragments (1.5 × 107 bp per genome/100 bp per Okazaki fragment) during S phase, it is not surprising that multiple nucleases are involved in OFP.
Our genetic analysis supports the notion that Dna2p processes a specific subset of Okazaki fragment flaps—namely, those arising from excessive strand displacement by pol δ. We showed not only that dna2 is synthetically lethal with a mutant of pol δ that gives abnormally high levels of strand displacement, but also that dna2 is suppressed by a mutant that gives decreased strand displacement. pol3–01 is a mutation that inactivates the 3′ to 5′ exonuclease function of pol δ. This nuclease has three known functions . First, because pol3–01 is a strong mutator, we can surmise that one function is in proofreading during DNA polymerization. Second, negative synergy of pol3–01 with msh2Δ and exo1Δ suggests a role in mismatch repair. These two activities are probably not relevant to the dna2 pol3–01 synthetic lethality since dna2–1 is not a mutator mutation and thus catastrophic mutagenesis is not likely to occur in the dna2 pol3–01 mutant (; unpublished data). OFP is the third process proposed to require pol δ exonuclease, since pol3–01 is synthetically lethal with rad27Δ . Mechanistically, this has been rationalized by the fact that pol δ 3′ exonuclease is an inhibitor of the strand displacement activity of pol δ during in vitro DNA synthesis . In the absence of its 3′ exonuclease, pol δ can no longer idle at a nick, since the 3′ displaced flaps that form as intermediates in idling cannot be removed nucleolytically. Instead, pol δ processively displaces the strand having a 5′ terminus . This is consistent with the idea of dna2 pol3–01 lethality stemming from the failure to efficiently process excessively long 5′ flaps on Okazaki fragments. pol δ-DV is another exonuclease-deficient allele of pol δ, and rad27-d is a proliferating cell nuclease antigen noninteracting mutant of rad27. Overproduction of Dna2p suppresses the lethality of a pol3 d-DV rad27-p rad51Δ strain , supporting our conclusions.
dna2–1 and dna2–2 mutants are suppressed by deleting the nonessential POL32-encoded subunit of pol δ that is required for optimum strand displacement. Pol32p is required for efficient in vitro DNA replication by pol δ in the presence of replication factor C, proliferating cell nuclease antigen, and a primed template. The ability to displace 5′ ends is drastically decreased for pol δ lacking Pol32p [35,43], and the pol δ complex is expected to be defective in strand displacement synthesis in pol32Δ strains, thus reducing the need for Dna2p. Pol32p has also been shown to interact with pol α, and the same mutant that is synthetically lethal with dna2–2, pol1–1 (G493R), is synthetically sick with pol32Δ . This might hint at coordination between Okazaki fragment initiation and elongation, although there is no reported phenotype associated with a mutation (pol32–8) that disrupts the Pol32p–pol α interaction. While this manuscript was being prepared, it was reported that a mutation in cdc27+, the Schizosaccharomyces pombe ortholog of POL32, suppresses one allele of S. pombe dna2 , so this suppression is conserved. Why is pol32Δ rad27Δ lethal  while pol32Δ dna2 grows more robustly than dna2 mutants? Loss of pol32Δ may shift the course of OFP from a flap removal pathway to one employing RNaseH. In the RNaseH pathway, FEN1 exonuclease may become essential to remove the last ribonucleotide, an activity that Dna2p does not appear to possess.
Interaction with Pol α and Primase (and Mcm10p).
Our demonstration here of synthetic lethal interactions of dna2 with pol1–1 and with pri1-M4, components of pol α–primase, may also fortify the argument that Dna2p participates in OFP. Although pri1-m4 has an S phase checkpoint defect in addition to a DNA replication defect, the replication defect is more likely to be responsible for the synthetic lethality with dna2, given our data that dna2 is not synthetically lethal with any of the mutations in major checkpoint genes, including mec1Δ sml1Δ. Certain alleles of dna2 are also synthetically lethal with mcm10–1 . Recently, MCM10 has been implicated in elongation and in stabilizing pol α in vivo as well as in stimulating pol α in vitro [84,85]. The interdependent functions of DNA2 and MCM10 may reflect an interaction in lagging-strand replication. Alternatively, Dna2p might play a role in repair of mcm10–1-generated damage. The combined new data on interactions between Dna2p, pol Δ, pol α, and primase may be evidence for a previously unexpected coupling of primer synthesis, polymerase switching, and primer removal.
Differences between the Genetic Interactions of DNA2 and Those of RAD27
Comparison of the data presented here (see Figure 2; Table S2) and the results of a similar thoroughly validated SGA screen using rad27Δ as a query gene  reveals a wide (and unanticipated) divergence between genes that are synthetically lethal with dna2 and those that are synthetically lethal with rad27Δ. This divergence implies that the two enzymes may have slightly different sets of substrates. As pointed out in Results, the synthetic lethality of rad27Δ [69,87], but not dna2, with checkpoint mutants and with recombination mutants suggests that rad27Δ mutants probably accumulate more single-stranded DNA (the proposed signal for checkpoint activation) and more DSBs (repaired by recombination) , than dna2 mutants do. If dna2 mutants accumulate less damage than rad27Δ mutants, this in turn might suggest that FEN1 is the major OFP nuclease and that Dna2p is required at fewer (or different) sites than FEN1 . This conclusion is consistent with other previously published evidence (despite the potential conundrum that deleting DNA2 is lethal and deleting RAD27 is not). First, pol δ, proliferating cell nuclease antigen, and FEN1 appear to act in a highly concerted fashion on templates that are optimal for pol δ efficiency in vitro, with little evidence that flaps longer than a few nucleotides are ever produced [10,33]. Second, dna2 mutants are weak mutators, while rad27Δ mutants are strong mutators, as measured by point mutations or stability of di- or trinucleotide repeats, or even larger repeats [16,87,89–91]. Dna2p may be specialized to function in OFP in genomic locations where the DNA sequence poses problems for pol δ, creating flaps that are not good substrates for FEN1. These regions are likely to include the rDNA and telomeres, since we have shown significant replication defects in these loci in dna2 mutants [17,35–37]. The role of Dna2p is not likely to be limited to these regions, however, as our previous immunofluorescence and chromatin immunoprecipitation analyses show Dna2p to be located at many other genomic regions during S phase . Possible sites are replication slow zones or the inverted Ty repeats that give rise to genomic instability.
If FEN1 is the major flap nuclease, either Dna2p might help FEN1 on some flaps or Dna2p might recognize discrete subsets of flaps and process them independently. The genetic differences observed, combined with the quantitative biochemical data that show that Dna2p is very inefficient at stimulating FEN1, even on long flaps, direct future attention to potentially independent roles for Dna2p and FEN1.
The Helicase Network for Preserving Genomic Stability
Previous screening of the nonessential gene knock-out collection with sgs1Δ and srs2Δ as queries identified a so-called helicase network defined by a set of common interactions . Genes in this network are implicated by dozens of recent studies in the repair of damaged replication forks through sister chromatid recombination and replication restart mechanisms, but coupling of the network to DNA replication remains poorly understood (e.g., ). By reversing the screening process and using an essential lagging-strand replication gene as query, we have found that dna2 is synthetically lethal with mutations in all of these helicases and in the genes with which they interact. The interactions shown in Figures 1 and 2 and our subsequent work (see Table 2) suggest that Dna2p may be one of the major replication proteins that coordinate this helicase network and replication.
The synthetic lethality of dna2–2 sgs1Δ and its lack of suppression by rad51Δ (Table 2) suggests that Sgs1p participates directly in DNA replication by aiding Dna2p in stimulating flap cleavage during OFP under some circumstances. This interpretation is attractive since the human Sgs1p orthologs, BLM and WRN, interact physically with Dna2p and suppress the replication defects in dna2–1 mutants when overexpressed in yeast [94,95]. (The suppression could not be investigated with yeast SGS1, since its overproduction is toxic [A. Morgan, personal communication; unpublished data].) The reproducible effect of deleting RAD51 in restoring defective growth to dna2–2 sgs1Δ mutants, however, is consistent with SGS1 playing an additional role in a late stage of recombinational repair of dna2-induced lesions, such as in the resolution of Holliday junction intermediates into viable products, a reaction that requires Sgs1p in conjunction with Top3p . Dna2p might be required to remove 5′ ends of nascent DNA in reversed forks, while Sgs1p serves as a helicase to resolve the forks, like RecJ and RecQ in bacteria . Lesions due to DNA2 deficiency do have the potential to lead to lethal recombination intermediates, because we find that dna2 is synthetically lethal with srs2Δ and that this lethality is efficiently suppressed by deleting RAD51. SRS2, given its role in inhibiting an early step in recombination [98–100], probably prevents dna2–2 sgs1Δ–derived lesions from entering the recombinational repair pathway. Thus, the interaction between Sgs1p and Dna2p is multipotential.
Rrm3p promotes DNA replication through non-nucleosomal protein–DNA complexes, including the rDNA RFB, Rap1p-binding sites in the telomere, inactive ARS sites, and centromere DNA [101–104]. Rrm3p may act on the same replication intermediates as Dna2p rather than on downstream toxic intermediates formed during repair of faulty replication, since rad51 mutations do not suppress the dna2–2 rrm3Δ synthetic lethality. Recently, rrm3Δ has been tested for synthetic lethality against a number of candidate gene deletions. Unlike dna2–2 rrm3Δ, most of the synthetically lethal combinations, such as rrm3Δ srs2Δ and rrm3Δ sgs1Δ, were suppressed by recombination mutants [47,75]. Thus, early replication intermediates cause cell lethality in dna2 rrm3Δ mutants, while recombination intermediates cause cell lethality in rrm3Δ sgs1Δ mutants. These intermediates may not involve FEN1, since rad27Δ rrm3Δ is not synthetically lethal . Although both dna2 and rrm3Δ mutants show significant pausing in the rDNA, a fob1Δ mutation did not restore viability to the dna2–2 rrm3Δ mutant (Table 2), so there are additional sequences replicated by these genes. We have recently demonstrated that Dna2p can stimulate FEN1 cleavage of long flaps with secondary structure, but that the reaction is inefficient [12,105]. Since Rrm3p is a 5′ to 3′ helicase, Rrm3p is a candidate for a helicase that may aid Dna2p in flap processing.
It does not appear that dna2 interacts with genes involved in nucleotide excision repair, consistent with the relative resistance of dna2 mutants to UV irradiation . Base excision repair (long patch) involves all of the proteins also involved in OFP, and therefore a role for Dna2p in base excision repair would be supported by the interactions found in this work and the MMS sensitivity of dna2 mutants . A role for Dna2p in an unidentified Rad52p-dependent pathway was discussed above.
The synthetic lethality of dna2 with each member of the Mre11p complex may contribute to emerging evidence that the Mre11p complex functions at the replication fork. First, the seven genes whose interactions overlapped most significantly with dna2 (see Figure 2) are also synthetically lethal with the genes of the Mre11p complex. Second, the Mre11p complex associates with chromatin primarily during S phase, and this association does not appear to require DSBs . It has been suggested that the Mre11p complex assists sister chromatid association , and that this association is required for recombinational repair of DSBs during DNA replication. Another view derives from the fact that the Mre11p complex and Exo1p are both required for activation of the Rad53p checkpoint kinase after inhibition of replication by HU. This leads to the inference that the Mre11p complex and Exo1p may convert DSBs arising at stalled replication forks into single-stranded DNA, a signal for subsequent repair. Since replication forks stall in dna2 mutants, the synthetic lethality with mutations in Mre11p complex components or Exo1p could be explained by a failure to produce the single-stranded DNA signal. It is possible that DSBs that arise during normal DNA replication are repaired in Mre11p complex mutants, but are lethal in cells lacking both Mre11p and Dna2p . Finally, or alternatively, the synthetic lethality of dna2 mre11Δ may indicate that DNA2 is involved in a telomere defect, as has been shown in S. pombe [48,107]. We cannot eliminate the possibility that the Dna2p/Mre11p interaction is involved in repair, but we note that dna2–2 and rad50–5, which is as deficient in repair as rad52Δ, are epistatic with respect to repair.
Sister Chromatid Cohesion
Replication forks in dna2 mutants pause at the RFB in the rDNA, where DSBs result [17,35,37]. Our current finding that deletion of FOB1 suppresses the synthetic lethality of dna2–2 ctf4Δ damage could be explained if Ctf4p-mediated sister chromatid cohesion is necessary to repair damage at the RFB due to defective Dna2p. This requirement for cohesion in DNA2 mutants is not limited to the rDNA, since the dna2–2 ctf4Δ fob1Δ strain is ts and radiation sensitive. Thus, the Dna2p deficiency must give rise to damage requiring cohesion for repair elsewhere in the chromosome as well. A role for cohesion in maintaining the replication fork during stalling or collapse is attractive since cohesion is required for efficient DSB repair ; a role for sister chromatid cohesion in preventing excessive sister chromatid exchange due to breaks at the RFB has been directly demonstrated . We note that our analysis of replication in dna2–2 strains by two-dimensional gel electrophoresis indicates a high incidence of stalled replication forks at sequences throughout the rDNA, not limited to the RFB, suggesting general replication fork stalling in dna2 mutants and providing physical evidence of a more delocalized requirement for sister chromatid cohesion, perhaps throughout the chromosome [35,37].
Chromatin Remodeling, Disassembly, and Reassembly
During DNA replication, the chromatin in front of the replication fork is disassembled and then reassembled behind the fork. Our new findings add to recent findings from many sources that are providing the first insight into the molecular links between the replication machinery and chromatin dynamics. Dna2p interacts with both Asf1p and yFACT. The Asf1p/Dna2p interaction in chromatin assembly/remodeling is too ill-defined for further inferences at the moment. However, the allele-specific synthetic lethality between dna2 and pol1–1 suggests that Dna2p participates in the recently demonstrated interplay between Spt16p (a component of the FACT-like nucleosome reorganization factor), Ctf4p, and pol α . The pol1–1 mutant protein fails to interact with Spt16p and shows altered temporal interaction with Ctf4p. The compromised association between Spt16p and pol α in the pol1–1 mutant is accompanied by a delay both in pol α recruitment to late origins and its release, leading to a slow S phase . By adding a link between Dna2p and this particular aspect of pol α function, our results support the model of the Formosa lab that the yeast Spt16p complex is likely to be directly involved in DNA replication [52–54,110], as has also been suggested for the frog FACT complex . The Spt16p remodeling complex may facilitate the movement of pol α and Dna2p through nucleosomes, as proposed for human FACT in transcription [112,113]. The caveat that yFACT defects might result in reduced transcription of gene(s) that interact with DNA2 was mentioned above.
Other DNA2-interacting genes encode specific sets of histone modification enzymes that catalyze histone ubiquitylation, methylation, and deacetylation. We discovered here that the synthetic sickness of dna2 and rad6 is related to Rad6p/Bre1p-mediated ubiquitylation of histone H2B, which in turn leads to methylation of H3 at the lysine at position 4 by the SET1 complex, containing Set1p, Swd1p, and Swd3p . set1 mutants are sensitive to HU and may accumulate DNA damage during S phase. set1 was not recovered in the SGA screen because it is not in the deletion collection. Ubiquitylation and methylation alter silencing and chromatin structure at the rDNA and at telomeres, which may suggest a mechanism for interaction with dna2 [114,115].
The interaction between Dna2p and Hst3p and the Rpd3p/Pho23p histone deacetylases is interesting because mutations in ORC, the replication initiator, as well as rad27Δ, pol32Δ, and sgs1Δ also show synthetic interaction with hst3Δ [18,116]. HST3 performs a redundant function in DNA replication but hst3 hst4 cells have phenotypes indicative of replication defects, such as increased rates of chromosome loss and mitotic recombination, decreased telomere silencing, and hypersensitivity to UV treatment . DNA2 is the only DNA replication gene thus far found to interact with RPD3. Histone deacetylation by the Rpd3p/Pho23p complex has been previously implicated in the temporal regulation of origin activation, but not elongation, in DNA replication [67,68]. The observation that hst1Δ, hst3Δ, rpd3Δ, bre1Δ, spt16Δ, cac2Δ, and vid21Δ are all linked to the network shown in Figure 2 suggests that the existing nucleosome structure has been optimized for high fidelity DNA replication (see Protocol S1).
We propose that the genome maintenance network is coordinated by physical interaction of the replication proteins with the complexes that carry out regulation and repair. A good deal of evidence supports this. Originally we found that Dna2p co-purified with FEN1 and later demonstrated the genetic interaction. We discovered a genetic interaction between Dna2p and BLM and WRN helicases—the human orthologs of Sgs1p—and then found that this genetic interaction also represented a direct physical interaction between Dna2p and those helicases . Others found genetic interactions between DNA2 and RFA1, encoding the single-stranded DNA binding protein RPA, and similarly documented a physical interaction between the proteins Dna2p and RPA [13,118]. Indeed, most DNA replication proteins multitask and are components of many complexes. Pol δ and ɛ are involved in DNA repair complexes as well as replication complexes. Pol ɛ is, in addition, involved in the S phase checkpoint. FEN1 itself is involved in multiple reactions, including long patch base excision repair . Though circumstantial, the resemblance between the basic topology of the genetic network described here and the proteomic network described in bacteria  further suggests the model that replication proteins physically coordinate repair and regulatory genome maintenance complexes. We anticipate that this model will be verified in detail when proteomic approaches in yeast yield the kind of comprehensive data that can already be obtained from genetic screens such as SGA.
Materials and Methods
All strains used in the study are found in Table S4. The strains used in the SGA screen and subsequent verification were as follows: 4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura30Δ; 4741 Mat α his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; strain 4741dna2–1–6D (MB100) MATa dna2-1his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; strain 4741dna2–2–11D (MB101) MATα dna2–2::LEU2 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; Y3656 can1Δ::MFA1pr-HIS3-Mfα1pr-LEU2 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; Y3656dna2–1; Y3656 dna2–1::URA3; Y3656dna2–2; Y3556 dna2–2::URA3; and W30W303 MATa ade2–1 can1–100 his3–11,15 leu2–3,112, trp1–1 ura3 RAD5. Y3656 is derived from strain 4741 as described . Unless otherwise noted, all haploid deletion mutants used in this work were in strain 4741 and all double mutants were tested using 4741dna2–1 or Y3656dna2–2 as indicated.
The SGA screen was performed as previously described . Y3656dna2–1 and Y356dna2–2 were constructed for this study and used as query strains. SGA analysis was performed for each of the dna2 alleles. Genes that showed synthetic lethality or synthetic sickness in the primary screen were tested by standard tetrad dissection . For the secondary tetrad analysis, new heterozygous diploids were constructed between MB100 (4741 dna2–1) and MB101 (4741 dna2–2) and each of the candidate deletion mutants in strain 4741 (Invitrogen, Carlsbad, California, United States). Thus, each synthetic interaction that is reported here was tested using two independent diploids, one in the original screen and one in the secondary screen. The dna2–2 dissections were incubated at 30 °C and dna2–1 dissections at 23 °C. Any strain failing to generate viable double mutants was deemed synthetically lethal. A strain was considered synthetically sick if tetrads gave fewer double mutants than expected and double mutants grew slower than either single mutant. At least ten tetrads were dissected for each double mutant, and usually more were dissected.
X-ray and MMS sensitivity.
The X-ray source was Pantak (East Haven, Connecticut, United States) MK II 70 kev 20 ma. The source was calibrated and experiments were carried out as previously described . For the MMS sensitivity assay, 0.005% and 0.01% MMS was added to yeast-peptone-dextrose (YPD) plates after autoclaving, and plates were used the same day.
Protocol S1. Other
(53 KB DOC)
Table S1. Genes That Show Little, If Any, Interaction with dna2–1 or dna2–2 in Secondary Screen
(107 KB PDF)
(111 KB PDF)
Table S3. Number of Spores after Dissection of dna2–2 mec1 tel1 slm1 Heterozygote
(95 KB PDF)
Table S4. Strains Used
(103 KB PDF)
This work was supported in part by United States Public Health Service grant GM25508 to JLC and by a National Science and Engineering Research Council of Canada grant, a grant from the Canadian Institutes of Health Research, and funds from Genome Canada, the Ontario Genome Institute, and the Ontario Research and Development Challenge Fund to CB.
MEB, AHYT, PP, CB, and JLC conceived and designed the experiments. MEB, AHYT, PP, and XP performed the experiments. MEB, AHYT, PP, CB, and JLC analyzed the data. MEB, CB, and JLC wrote the paper.
- 1. Kuo CL, Huang CH, Campbell JL (1983) Isolation of yeast DNA replication mutants using permeabilized cells. Proc Natl Acad Sci U S A 80: 6465–6469.
- 2. Budd ME, Campbell JL (1995) A new yeast gene required for DNA replication encodes a protein with homology to DNA helicases. Proc Natl Acad Sci U S A 92: 7642–7646.
- 3. Budd ME, Choe WC, Campbell JL (1995) DNA2 encodes a DNA helicase essential for replication of eukaryotic chromosomes. J Biol Chem 270: 26766–26769.
- 4. Bae SH, Choi E, Lee K, Park J, Lee S, et al. (1998) Dna2 of Saccharomyces cerevisiae possesses a single-stranded DNA-specific endonuclease activity that is able to act on double-stranded DNA in the presence of ATP. J Biol Chem 273: 26880–26890.
- 5. Waga S, Bauer G, Stillman B (1994) Reconstitution of complete SV40 DNA replication with purified replication factors. J Biol Chem 269: 10923–10934.
- 6. Harrington JJ, Lieber MR (1994) The characterization of a mammalian DNA structure-specific endonuclease. EMBO J 13: 1235–1246.
- 7. Budd ME, Campbell JL (1997) A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol 17: 2136–2142.
- 8. Formosa T, Nittis T (1999) Dna2 mutants reveal interactions with DNA polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full DNA2 helicase activity is not essential for growth. Genetics 151: 1459–1470.
- 9. Bae SH, Seo YS (2000) Characterization of the enzymatic properties of the yeast Dna2 helicase/endonuclease suggests a new model for Okazaki fragment processing. J Biol Chem 275: 38022–38031.
- 10. Ayyagari R, Gomes XV, Gordenin DA, Burgers PMJ (2003) Okazaki fragment maturation in yeast. I. Distribution of functions between Fen1 and Dna2. J Biol Chem 278: 1618–1625.
- 11. Jin YH, Ayyagari R, Resnick MA, Gordenin DA, Burgers PMJ (2003) Okazaki fragment maturation in yeast. II. Cooperation between the polymerase and 3′ to 5′ exonuclease activities of Pol delta in the creation of a ligatable nick. J Biol Chem 278: 1626–1633.
- 12. Kao HI, Veeraraghavan J, Polaczek P, Campbell JL, Bambara RA (2004) On the roles of Saccharomyces cerevisiae Dna2p and FEN1 in Okazaki fragment processing. J Biol Chem 279: 15014–15024.
- 13. Bae SH, Bae KH, Kim JA, Seo YS (2001) RPA governs endonuclease switching during processing of Okazaki fragments in eukaryotes. Nature 412: 456–461.
- 14. Kao HI, Campbell JL, Bambara RA (2004) Dna2p helicase/nuclease is a tracking protein, like FEN1, for flap cleavage during Okazaki fragment maturation. J Biol Chem 279: 50840–50849.
- 15. Toueille M, Hubscher U (2004) Regulation of the DNA replication fork: A way to fight genomic instability. Chromosoma 113: 113–125.
- 16. Budd ME, Campbell JL (2000) The pattern of sensitivity of yeast dna2 mutants to DNA damaging agents suggests a role in DSB and postreplication repair pathways. Mutat Res 459: 173–186.
- 17. Choe W, Budd M, Imamura O, Hoopes L, Campbell JL (2002) Dynamic localization of an Okazaki fragment processing protein suggests a novel role in telomere replication. Mol Cell Biol 22: 2002–2017.
- 18. Tong AHY, Lesage G, Bader GD, Ding H, Xu H, et al. (2004) Global mapping of the yeast genetic interaction network. Science 303: 808–813.
- 19. Tong AHY, Evangelista M, Parsons AB, Xu , Bader GD, et al. (2001) Systematic genetic analysis with ordered arrays of yeast deletion mutants. Science 294: 2364–2368.
- 20. Hanna JS, Kroll ES, Lundblad V, Spencer FA (2001) Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion. Mol Cell Biol 21: 3144–3158.
- 21. Budd ME, Choe WC, Campbell JL (2000) The nuclease activity of the yeast Dna2 protein, which is related to the RecB-like nucleases, is essential in vivo. J Biol Chem 275: 16518–16529.
- 22. Subramanya HS, Bird LE, Brannigan JA, Wigley DB (1996) Crystal structure of a DExx box DNA helicase. Nature 384: 379–383.
- 23. Araki Y, Kawasaki Y, Sasanuma H, Tye BK, Sugino A (2003) Budding yeast mcm10/dna43 mutant requires a novel repair pathway for viability. Genes Cells 8: 465–480.
- 24. Ireland MJ, Reinke SS, Livingston DM (2000) The impact of lagging strand replication mutations on the stability of CAG repeat tracts in yeast. Genetics 155: 1657–1665.
- 25. Frank G, Braunshofer-Reiter C, Wintersberger U (1998) Yeast RNase H(35) is the counterpart of the mammalian RNase HI and is evolutionarily related to prokaryotic RNase HII. FEBS Lett 421: 23–26.
- 26. Johnson RE, Kovvali GK, Prakash L, Prakash S (1998) Role of yeast Rth1 nuclease and its homologs in mutation avoidance, DNA repair, and DNA replication. Curr Genet 34: 21–29.
- 27. Tishkoff DX, Boerger AL, Bertrand P, Filosi N, Gaida GM, et al. (1997) Identification and characterization of Saccharomyuces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2. Proc Natl Acad Sci U S A 94: 7487–7492.
- 28. Parenteau J, Wellinger RJ (1999) Accumulation of single-stranded DNA and destabilization of telomeric repeats in yeast mutant strains carrying a deletion of RAD27. Mol Cell Biol 19: 4143–4152.
- 29. Tran PT, Erdeniz N, Dudley S, Liskay RM (2002) Characterization of nuclease-dependent functions of Exo1p in Saccharomyces cerevisiae. DNA Repair (Amst) 1: 895–912.
- 30. Smolikov S, Mazor Y, Krauskopf A (2004) ELG1, a regulator of genome stability, has a role in telomere length regulation and in silencing. Proc Natl Acad Sci U S A 101: 1656–1661.
- 31. Kanellis P, Agyel R, Durocher D (2003) Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability. Curr Biol 13: 1583–1595.
- 32. Bellaoui M, Chang M, Ou J, Xu H, Boone C, et al. (2003) Elg1 forms an alternative RFC complex important for DNA replication and genome integrity. EMBO J 22: 4304–4313.
- 33. Garg P, Stith CM, Sabouri N, Johansson E, Burgers PM (2004) Idling by DNA polymerase delta maintains a ligatable nick during lagging-strand DNA replication. Genes Dev 18: 2764–2773.
- 34. Johansson E, Garg P, Burgers PMJ (2004) The Pol32 subunit of DNA polymerase delta contains separable domains for processive replication and proliferating cell nuclear antigen (PCNA) binding. J Biol Chem 279: 1907–1915.
- 35. Weitao T, Budd M, Mays Hoopes LL, Campbell JL (2003) Dna2 helicase/nuclease causes replicative fork stalling and double-strand breaks in the ribosomal DNA of Saccharomyces cerevisiae. J Biol Chem 278: 22513–22522.
- 36. Mays Hoopes LL, Budd M, Choe W, Weitao T, Campbell JL (2002) Mutations in DNA replication genes reduce yeast life span. Mol Cell Biol 22: 4136–4146.
- 37. Weitao T, Budd M, Campbell JL (2003) Evidence that yeast SGS1, DNA2, SRS2, and FOB1 interact to maintain rDNA stability. Mutat Res 532: 157–172.
- 38. Gangloff S, Soustelle C, Fabre F (2000) Homologous recombination is responsible for cell death in the absence of the Sgs1 and Srs2 helicases. Nat Genet 25: 192–194.
- 39. Mullen JR, Kaliraman V, Ibrahim SS, Brill SJ (2001) Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae. Genetics 157: 103–118.
- 40. Hryciw T, Tang M, Fontanie T, Xiao W (2002) MMS1 protects against replication-dependent DNA damage in Saccharomyces cerevisiae. Mol Genet Genomics 266: 848–857.
- 41. Symington LS (2002) Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66: 630–670.
- 42. Llorente B, Symington LS (2004) The Mre11 nuclease is not required for 5′ to 3′ resection at multiple HO-induced double-strand breaks. Mol Cell Biol 24: 9682–9694.
- 43. Fiorentino DF, Crabtree GR (1997) Characterization of Saccharomyces cerevisiae dna2 mutants suggests a role for the helicase late in S phase. Mol Biol Cell 8: 2519–2537.
- 44. Alcasabas AA, Osborn AJ, Bachant J, Hu F, Werler PJH, et al. (2001) Mrc1 transduces signals of DNA replication stress to activiate Rad53. Nat Cell Biol 3: 958–965.
- 45. Osborn AJ, Elledge SJ (2003) Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. Genes Dev 17: 1755–1767.
- 46. Katou Y, Kanoh Y, Bando M, Noguchi H, Tanaka H, et al. (2003) S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424: 1078–1083.
- 47. Torres JZ, Schnakenberg SL, Zakian VA (2004) Saccharomyces cerevisiae Rrm3p DNA helicase promotes genome integrity by preventing replication fork stalling: Viability of rrm3 cells requires the intra-S-phase checkpoint and fork restart activities. Mol Cell Biol 24: 3198–3212.
- 48. Lisby M, Barlow JH, Burgess RC, Rothstein R (2004) Choreography of the DNA damage response: Spatiotemporal relationships among checkpoint and repair proteins. Cell 118: 699–713.
- 49. Ritchie KB, Mallory JC, Petes TD (1999) Interactions of TLC1 (which encodes the RNA subunit of telomerase), TEL1, and MEC1 in regulating telomere length in the yeast Saccharomyces cerevisiae. Mol Cell Biol 19: 6065–6075.
- 50. Miles J, Formosa T (1992) Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo. Mol Cell Biol 12: 5274–5735.
- 51. Edwards SE, Li CX, Levy DL, Brown J, Snow PM, et al. (2003) Saccharomyces cervisiae DNA polymerase epsilon and polymerase sigma interact physically and functionally, suggesting a role for polymerase epsilon in sister chromatid cohesion. Mol Cell Biol 23: 2733–2748.
- 52. Schlesinger MB, Formosa T (2000) POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae. Genetics 155: 1593–1606.
- 53. Formosa T, Eriksson P, Wittmeyer J, Ginn J, Yu Y, et al. (2001) Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN. EMBO J 20: 3506–3517.
- 54. Rhoades AR, Ruone S, Formosa T (2004) Structural features of nucleosomes reorganized by yeast FACT and its HMG box component, Nhp6. Mol Cell Biol 24: 3907–3917.
- 55. Formosa T, Ruone S, Adams MD, Olsen AE, Eriksson P, et al. (2002) Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: Polymerase passage may degrade chromatin structure. Genetics 162: 1557–1571.
- 56. Zhou Y, Wang TSF (2004) A coordinated temporal interplay of nucleosome reorganization factor, sister chromatin cohesion factor, and DNA polymerase alpha facilitates DNA replication. Mol Cell Biol 24: 9568–9579.
- 57. Budd M, Campbell JL (1987) Temperature sensitive mutants of yeast DNA polymerase I. Proc Natl Acad Sci U S A 84: 2838–2842.
- 58. Pellicioli A, Lucca C, Liberi G, Marini F, Lopes M, et al. (1999) Activation of Rad53 kinase in response to DNA damage and its effect in modulating phosphorylation of the lagging strand DNA polymerase. EMBO J 18: 6561–6572.
- 59. Verreault A (2003) Histone deposition at the replication fork: A matter of urgency. Mol Cell 11: 283–284.
- 60. Prakash L (1981) Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3, and rad52 mutations. Mol Gen Genet 184: 471–478.
- 61. Sung PE, Berleth E, Prakash S, Prakash L (1991) Yeast RAD6 encoded ubiquitin conjugating enayme mediates protein degradation dependent on the N-end-recognizing E3 enzyme. EMBO J 10: 2187–2193.
- 62. Wood A, Krogan NJ, Dover J, Schneider J, Heidt J, et al. (2003) Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter. Mol Cell 11: 267–274.
- 63. Bailly V, Lamb J, Sung P, Prakash S, Prakash L (1994) Specific complex formation between yeast RAD6 and RAD18 proteins: A potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites. Genes Dev 8: 811–820.
- 64. Ulrich HD, Jentsch S (2000) Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair. EMBO J 19: 3388–3397.
- 65. Hwang WW, Venkatasubrahmanyam S, Ianculescu AG, Tong A, Boone C, et al. (2003) A conserved RING finger protein required for histone H2B monoubiquitination and cell size control. Mol Cell 11: 261–266.
- 66. Ng HH, Dole S, Struhl K (2003) The Rtf1 component of the Paf1 transcriptional elongation complex is required for ubiquitination of histone H2B. J Biol Chem 278: 33625–33628.
- 67. Vogelauer M, Rubbi L, Lucas I, Brewer BJ, Grunstein M (2002) Histone acetylation regulates the time of replication origin firing. Mol Cell 10: 1223–1233.
- 68. Aparicio JG, Viggiani CJ, Gibson DG, Aparicio OM (2004) The Rpd3-Sin3 histone deacetylase regulates replication timing and enables intra-S origin control in Saccharomyces cerevisiae. Mol Cell Biol 24: 4769–4780.
- 69. Myung K, Datta A, Chen C, Kolodner RD (2001) SGS1, the Saccharomyces cerevisiae homologue of BLM and WRN, suppresses genome instability and homeologous recombination. Nat Genet 27: 113–116.
- 70. Myung K, Datta A, Kolodner RD (2001) Suppression of spontaneous chromosomal rearrangements by S phase checkpoint functions in Saccharomyces cerevisiae. Cell 104: 397–408.
- 71. Myung K, Chen C, Kolodner RD (2001) Multiple pathways cooperate in the suppression of genome instability in Saccharomyces cerevisiae. Nature 411: 1073–1076.
- 72. Myung K, Pennaneach V, Kats ES, Kolodner RD (2003) Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stability. Proc Natl Acad Sci U S A 100: 6640–6645.
- 73. Myung K, Smith S, Kolodner RD (2004) Mitotic checkpoint function in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 101: 15980–15985.
- 74. Pennaneach V, Kolodner RD (2004) Recombination and the Tel1 and Mec1 checkpoints differentially effect genome rearrangements driven by telomere dysfunction in yeast. Nat Genet 36: 612–617.
- 75. Schmidt KH, Kolodner RD (2004) Requirement of Rrm3 helicase for repair of spontaneous DNA lesions in cells lacking Srs2 or Sgs1 helicase. Mol Cell Biol 24: 3213–3226.
- 76. Butland G, Peregrin-Alvarez JM, Li JL, Yang W, Yang X, et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531–537.
- 77. Qiu J, Qian Y, Frank P, Wintersberger U, Shen B (1999) Saccharomyces cerevisiae RNase H(35) functions in RNA primer removal during lagging-strand DNA synthesis, most efficiently in cooperation with Rad27 nuclease. Mol Cell Biol 19: 8361–8371.
- 78. Huertas P, Aguilera A (2003) Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination. Mol Cell 12: 711–721.
- 79. Askree SH, Yehuda T, Smolikov S, Gurevich R, Hawk J, et al. (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101: 8658–8663.
- 80. Loog M, Morgan DO (2005) Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates. Nature 434: 104.
- 81. Jin YH, Obert R, Burgers PMJ, Kunkel TA, Resnick MA, et al. (2001) The 3′-5′ exonuclease of DNA polymerase delta can substitute for the 5′ flap endonuclease Rad27/Fen1 in processing Okazaki fragments and preventing genome instability. Proc Natl Acad Sci U S A 98: 5122–5127.
- 82. Huang ME, de Calignon A, Nicolas A, Galibert F (2000) POL32, a subunit of the Saccharomyces cerevisiae DNA polymerase delta, defines a link between DNA replication and the mutagenic bypass repair pathway. Curr Genet 38: 178–187.
- 83. Tanaka H, Ryu GH, Seo YS, MacNeill SA (2004) Genetics of lagging strand DNA synthesis and maturation in fission yeast: Suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta. Nucleic Acids Res 32: 6367–6377.
- 84. Ricke RM, Bielinsky AK (2004) Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha. Mol Cell 16: 173–185.
- 85. Fien K, Cho YS, Lee JK, Raychaudhuri S, Tappin I, et al. (2004) Primer utilization by DNA polymerase alpha-primase is influenced by its interaction with Mcm10p. J Biol Chem 279: 16144–16153.
- 86. Loeillet S, Palancade B, Cartron M, Thierry A, Fichard GF, et al. (2005) Genetic network interactions among replication repair and nuclear pore deficiencies in yeast. DNA Repair (Amst) 4: 459–468.
- 87. Tishkoff DX, Filosi N, Gaida GM, Kolodner RD (1997) A novel mutation avoidance mechanism dependent on Saccharomyces cerevisiae RAD27 is distinct from DNA mismatch repair. Cell 88: 253–263.
- 88. Liu Y, Kao HI, Bambara RA (2004) Flap endonuclease 1: A central component of DNA metabolism. Annu Rev Biochem 73: 589–615.
- 89. Johnson RE, Gopala KK, Prakash L, Prakash S (1995) Requirement for the yeast RTH1 5′ to 3′ exonuclease for the stability of simple repetitive DNA. Science 269: 238–240.
- 90. Callahan JL, Andrews KJ, Zakian VA, Freudenreich CH (2003) Mutations in yeast replication proteins that increase CAG/CTG expansions also increase repeat fragility. Mol Cell Biol 23: 7849–7860.
- 91. Lopes J, Debrauwere H, Buard J, Nicolas A (2002) Instability of the human minisatellite CEB1 in rad27Δ and dna2–1 replication-deficient yeast cells. EMBO J 21: 3201–3211.
- 92. Ooi SL, Shoemaker DD, Boeke JD (2003) DNA helicase gene interaction network defined using synthetic lethality analyzed by microarray. Nat Genet 35: 277–286.
- 93. Liberi G, Maffioletti G, Lucca C, Chiolo I, Baryshnikova A, et al. (2005) Rad51-dependent DNA structures accumulate at damaged replication forks in sgs1 mutants defective in the yeast ortholog of BLM RecQ helicase. Genes Dev 19: 339–350.
- 94. Imamura O, Campbell JL (2003) The human Bloom syndrome gene suppresses the DNA replication and repair defects of yeast dna2 mutants. Proc Natl Acad Sci U S A 100: 8193–8198.
- 95. Sharma S, Sommers JA, Brosh RM Jr (2004) In vivo function of the conserved non-catalytic domain of Werner syndrome helicase in DNA replication. Hum Mol Genet 13: 2247–2261.
- 96. Oakley TJ, Hickson ID (2002) Defending genome integrity during S-phase: Putative roles for RecQ helicases and topoisomerases. DNA Repair (Amst) 1: 175–207.
- 97. Courcelle J, Hanawalt PC (1999) RecQ and RecJ process blocked replication forks prior to resumption of replication in UV-irradiated Escherichia coli. Mol Gen Genet 262: 543–551.
- 98. Schiestl RH, Prakash S, Prakash L (1990) The SRS2 suppressor of rad6 mutations of Saccharomyces cerevisiae acts by channeling DNA lesions into the RAD52 DNA repair pathway. Genetics 124: 817–831.
- 99. Veaute X, Jeusset J, Soustelle C, Kowalczykowski SC, Le Cam E, et al. (2003) The Srs2 helicase prevents recombination by disruption Rad51 nucleorotein filaments. Nature 423: 309–312.
- 100. Krejci L, Van Komen S, Li Y, Villemain J, Reddy MS, et al. (2003) DNA helicase Srs2 disrupts the Rad51 presynaptic filament. Nature 423: 305–309.
- 101. Ivessa AS, Zakian VA (2002) To fire or not to fire: Origin activation in Saccharomyces cerevisiae ribosomal DNA. Genes Dev 16: 2459–2464.
- 102. Ivessa AS, Zhou JQ, Schulz VP, Monson EK, Zakian VA (2002) Saccharomyces Rrm3p, a 5′ to 3′ DNA helicase that promotes replication fork progression through telomeric and subtelomeric DNA. Genes Dev 16: 1383–1396.
- 103. Ivessa AS, Lenzmeier BA, Bessler JB, Goudsouzian LK, Schnakenberg SL, et al. (2003) The Saccharomyces cerevisiae helicase Rrm3p facilitates replication past nonhistone protein-DNA complexes. Mol Cell 12: 1525–1536.
- 104. Makovets S, Herskowitz I, Blackburn EH (2004) Anatomy and dynamics of DNA replication fork movement in yeast telomeric regions. Mol Cell Biol 24: 4019–4031.
- 105. Kao HI, Henricksen LA, Liu Y, Bambara RA (2002) Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate. J Biol Chem 277: 14379–14389.
- 106. Mirzoeva OK, Petrini JHJ (2003) DNA replication-dependent nuclear dynamics of the Mre11 complex. Mol Cancer Res 1: 207–218.
- 107. Tomita K, Kibe T, Kang HY, Seo YS, Uritani M, et al. (2004) Fission yeast Dna2 is required for generation of the telomeric single-strand overhang. Mol Cell Biol 24: 9557–9567.
- 108. Sjogren C, Nasmyth K (2001) Sister chromatid cohesion is required for postreplicative double-strand break repair in Saccharomyces cerevisiae. Curr Biol 11: 991–995.
- 109. Kobayashi T, Horiuchi T, Tongalnkar P, Vu L, Nomura M (2004) SIR2 regulates recombination between different rDNA repeats, but not recombination within individual rRNA genes in yeast. Cell 117: 441–453.
- 110. Wittmeyer J, Formosa T (1997) The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein. Mol Cell Biol 17: 4178–4190.
- 111. Okuhara K, Ohta K, Seo H, Shioda M, Yamada T, et al. (1999) A DNA unwinding factor involved in DNA replication in cell-free extracts of Xenopus eggs. Curr Biol 9: 341–350.
- 112. Orphanides G, Wu WH, Lane WS, Hampsey M, Reinberg D (1999) The chromatin-specific transcrition elongation factor FACT comprises human ST16 and SSRP1 proteins. Nature 400: 284–288.
- 113. Orphanides G, LeRoy G, Chang CH, Luse DS, Reinberg D (1998) FACT, a factor that facilitates transcript elongation through nucleosomes. Cell 92: 105–116.
- 114. Nagy PL, Griesenbeck J, Kornberg RD, Cleary ML (2002) A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc Natl Acad Sci U S A 99: 90–94.
- 115. Krogan NJ, Dover J, Khorrami S, Greenblatt JF, Schneider J, et al. (2002) COMPASS, a histone H3 (lysine 4) methyltransferase required for telomeric silencing of gene expression. J Biol Chem 277: 10753–10755.
- 116. Suter B, Tong A, Chang M, Yu L, Brown GW, et al. (2004) The origin recognition complex links replication, sister chromatid cohesion and transcriptional silencing in Saccharomyces cerevisiae. Genetics 167: 579–591.
- 117. Brachmann CB, Sherman JM, Devine SE, Cameron EE, Pillus L, et al. (1995) The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability. Genes Dev 9: 2888–2902.
- 118. Bae KH, Kim HS, Bae SH, Kang HY, Brill S, et al. (2003) Bimodal interaction between replication-protein A and Dna2 is critical for Dna2 function both in vivo and in vitro. Nucleic Acids Res 31: 3006–3015.
- 119. Guthrie C, Fink G, editors. (1991) Guide to yeast genetics and molecular biology. New York: Academic Press. 933 p.
- 120. Morrison A, Sugino A (1992) Roles of POL3, POL2 and PMS1 genes in maintaining accurate DNA replication. Chromosoma 102: S147–S149.
- 121. Toyn JH, Gunyukzlu PL, White WH, Thompson LA, Hollis GF (2000) A counterselection for the tryptohpan pathway in yeast: 5-fluoroanthranilic acid resistance. Yeast 16: 553–560.