Table S1. Sequences of primers used in plasmid construction. 	
Primers used to amplify unc-60 genomic fragments	
Sequence	Constructed minigene cassettes	
5'-GGCTGCCGCTCTAGAGAAACTCAACTTGATTCTAT-3'	unc-60E1-E2A-RFP, unc-60E1-E3B-GFP	
5'-GCCATGGTGGGATCCAAAGATGTCTGCACATCTG-3'	unc-60E1-E2A-RFP	
5'-GCGACCGGTGGATCCCTGCACTCCTTTCCGTCCTC-3'	unc-60E1-E3B-GFP	
		
Primers used for mutagenesis	
Sequence	Constructed minigene cassettes	
5'-CTCTTCTTTTTGCCaAACCaAACCaAACCTATGTGTGCCTGTT-3'	unc-60E1-E2A-RFP-M1, unc-60E1-E3B-GFP-M1	
5'-AACAGGCACACATAGGTTtGGTTtGGTTtGGCAAAAAGAAGAG-3'	unc-60E1-E2A-RFP-M1, unc-60E1-E3B-GFP-M1	
5'-TAACCTAACCTATaTaTaCCTGTTTTCTAG-3'	unc-60E1-E2A-RFP-M2, unc-60E1-E3B-GFP-M2	
5'-CTAGAAAACAGGtAtAtATAGGTTAGGTTA-3'	unc-60E1-E2A-RFP-M2, unc-60E1-E3B-GFP-M2	
Lowercase indicates nucleotides different from wild type.