Unlinking the methylome pattern from nucleotide sequence, revealed by large-scale in vivo genome engineering and methylome editing in medaka fish
Fig 3
Randomly integrated genomic fragments could not autonomously determine their methylation state.
(A) Schematic diagram illustrating the capturing and processing of genomic fragments for the interrogation of their autonomy in methylation state determination. The blue segment represents genomic region that is endogenously hypomethylated. (B) Distributions of the methylation rates of CpGs on the integrated genomic fragments, (left) without- or (right) with- artificial methylation prior to injection. The distributions were displayed separately for CpGs that are endogenously (upper) hypomethylated and (lower) hypermethylated. Bin width = 1%. Note that the histograms in the upper panels (i.e. CpGs that are endogenously hypomethylated) strongly resemble those in the lower panels (i.e. CpGs that are endogenously hypermethylated). “N” denotes the number of CpGs in the corresponding histograms. Mean coverage = (left) 292× and (right) 208×.